mRNA_Ecto-sp13_S_contig8191.19543.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig8191.19543.1
Unique NamemRNA_Ecto-sp13_S_contig8191.19543.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig8191.19543.1 vs. uniprot
Match: D7FM14_ECTSI (DNA polymerase I (POL I) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FM14_ECTSI)

HSP 1 Score: 213 bits (543), Expect = 2.650e-65
Identity = 109/112 (97.32%), Postives = 110/112 (98.21%), Query Frame = 1 
Query:    1 MIKPRDSLVASPGYAFVSADYSQVEMRLMAHLSSDPDLLKVFADGGDVFRTIAAALKGRGKKAADITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEAFKIRQTVLKTY 336
            MIKPRDSLVASPGYAFVSADYSQVEMRLMAHLS DPDLLKVFADGGDVFRTIAAALKGRGKKA DITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEAFKIRQTVLKT+
Sbjct:  212 MIKPRDSLVASPGYAFVSADYSQVEMRLMAHLSGDPDLLKVFADGGDVFRTIAAALKGRGKKAEDITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEAFKIRQTVLKTF 323
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BLAST of mRNA_Ecto-sp13_S_contig8191.19543.1 vs. uniprot
Match: A0A6H5L306_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L306_9PHAE)

HSP 1 Score: 210 bits (534), Expect = 9.070e-61
Identity = 107/112 (95.54%), Postives = 108/112 (96.43%), Query Frame = 1 
Query:    1 MIKPRDSLVASPGYAFVSADYSQVEMRLMAHLSSDPDLLKVFADGGDVFRTIAAALKGRGKKAADITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEAFKIRQTVLKTY 336
            M+KPRDSLVASPGYAFVSADYSQVEMRLMAHLS DPDLLKVFADGGDVFRTIAAALKGRG KA DITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEAFKIRQTVL TY
Sbjct: 2012 MMKPRDSLVASPGYAFVSADYSQVEMRLMAHLSGDPDLLKVFADGGDVFRTIAAALKGRGNKAEDITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEAFKIRQTVLNTY 2123
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BLAST of mRNA_Ecto-sp13_S_contig8191.19543.1 vs. uniprot
Match: A0A7S3XVL9_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XVL9_HETAK)

HSP 1 Score: 102 bits (254), Expect = 4.490e-24
Identity = 55/111 (49.55%), Postives = 74/111 (66.67%), Query Frame = 1 
Query:    4 IKPRDSLVASPGYAFVSADYSQVEMRLMAHLSSDPDLLKVFADGGDVFRTIAAALKGRGKKAADITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEAFKIRQTVLKTY 336
            I PRD  +A+PG+  ++ADYSQVEMRLMAH S DP L  +F  GGDVFR IA+  + RG   A +T E R+  K V YG+LYG   +AL  +L +S  EA ++++  L+TY
Sbjct:   17 IIPRDFFMAAPGHILIAADYSQVEMRLMAHFSRDPGLQALFKSGGDVFRGIAS--RWRGVPEAAVTPELRKACKQVAYGILYGAGPNALRQELGVSYQEAERMQRRFLETY 125
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BLAST of mRNA_Ecto-sp13_S_contig8191.19543.1 vs. uniprot
Match: A0A536ZUN4_9PROT (DNA polymerase I n=1 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A536ZUN4_9PROT)

HSP 1 Score: 98.6 bits (244), Expect = 1.090e-21
Identity = 50/97 (51.55%), Postives = 66/97 (68.04%), Query Frame = 1 
Query:   13 RDSLVASPGYAFVSADYSQVEMRLMAHLSSDPDLLKVFADGGDVFRTIAAALKGRGKKAADITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEA 303
            R++ +ASPG+  VSADYSQVE+R+MAHLS D  LLK F+DG D+ R  AA + G    AAD+T ++RR  K V +G++YG SA  L  QL + R  A
Sbjct:  684 REAFIASPGHVLVSADYSQVELRIMAHLSEDASLLKAFSDGDDIHRATAAEIFG--VPAADVTADQRRYIKAVNFGLIYGMSAFGLAVQLNIERSAA 778
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BLAST of mRNA_Ecto-sp13_S_contig8191.19543.1 vs. uniprot
Match: A0A2E6G850_9GAMM (DNA polymerase I n=1 Tax=Porticoccaceae bacterium TaxID=2026782 RepID=A0A2E6G850_9GAMM)

HSP 1 Score: 95.5 bits (236), Expect = 1.310e-20
Identity = 46/97 (47.42%), Postives = 67/97 (69.07%), Query Frame = 1 
Query:   13 RDSLVASPGYAFVSADYSQVEMRLMAHLSSDPDLLKVFADGGDVFRTIAAALKGRGKKAADITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEA 303
            R++ +A+PGY  ++ADYSQ+E+R+MAHLS DP+L+  FA G DV RT AA +      A+ ITE +RR  K + +G++YG SA  L  QL ++R +A
Sbjct:  679 REAFIAAPGYKIIAADYSQIELRIMAHLSRDPNLIAAFAKGEDVHRTTAAEIFSA--PASQITESQRRSAKAINFGLIYGMSAFGLSKQLNIARKDA 773
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BLAST of mRNA_Ecto-sp13_S_contig8191.19543.1 vs. uniprot
Match: A0A3B8T129_9ALTE (DNA polymerase I (Fragment) n=1 Tax=Glaciecola sp. TaxID=1905676 RepID=A0A3B8T129_9ALTE)

HSP 1 Score: 95.1 bits (235), Expect = 1.760e-20
Identity = 50/110 (45.45%), Postives = 72/110 (65.45%), Query Frame = 1 
Query:    7 KPRDSLVASPGYAFVSADYSQVEMRLMAHLSSDPDLLKVFADGGDVFRTIAAALKGRGKKAADITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEAFKIRQTVLKTY 336
            K R++ +A PGY  V+ADYSQ+E+R+MAHLS DP LL  FA+G DV +  A+ + G   ++  +T E+RR +K + +G++YG SA  L  QL + R EA    QT + TY
Sbjct:  510 KVREAFIARPGYKIVAADYSQIELRIMAHLSKDPGLLHAFANGLDVHKATASEVFGVPLES--VTSEQRRSSKAINFGLIYGMSAFGLAKQLNIPRNEA----QTYMNTY 613
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BLAST of mRNA_Ecto-sp13_S_contig8191.19543.1 vs. uniprot
Match: A0A536SNA4_9PROT (DNA polymerase I n=1 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A536SNA4_9PROT)

HSP 1 Score: 95.1 bits (235), Expect = 1.780e-20
Identity = 49/97 (50.52%), Postives = 66/97 (68.04%), Query Frame = 1 
Query:   13 RDSLVASPGYAFVSADYSQVEMRLMAHLSSDPDLLKVFADGGDVFRTIAAALKGRGKKAADITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEA 303
            R++ VA  G+A VSADYSQVE+R+MAH+S D +LL+ FA G D+ R  AA + GR  K  ++T EERR  K++ +G++YG SA  L  QL L R  A
Sbjct:  680 REAFVAPKGHAIVSADYSQVELRIMAHISGDENLLRAFAAGEDIHRATAAEVFGRSLK--EVTPEERRYAKVINFGLIYGMSAFGLAQQLGLERATA 774
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BLAST of mRNA_Ecto-sp13_S_contig8191.19543.1 vs. uniprot
Match: A0A348MT94_9ALTE (DNA polymerase I n=1 Tax=Glaciecola sp. TaxID=1905676 RepID=A0A348MT94_9ALTE)

HSP 1 Score: 95.1 bits (235), Expect = 1.790e-20
Identity = 50/110 (45.45%), Postives = 72/110 (65.45%), Query Frame = 1 
Query:    7 KPRDSLVASPGYAFVSADYSQVEMRLMAHLSSDPDLLKVFADGGDVFRTIAAALKGRGKKAADITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEAFKIRQTVLKTY 336
            K R++ +A PGY  V+ADYSQ+E+R+MAHLS DP LL  FA+G DV +  A+ + G   ++  +T E+RR +K + +G++YG SA  L  QL + R EA    QT + TY
Sbjct:  706 KVREAFIARPGYKIVAADYSQIELRIMAHLSKDPGLLHAFANGLDVHKATASEVFGVPLES--VTSEQRRSSKAINFGLIYGMSAFGLAKQLNIPRNEA----QTYMNTY 809
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BLAST of mRNA_Ecto-sp13_S_contig8191.19543.1 vs. uniprot
Match: A0A2N5Y7L2_9GAMM (DNA polymerase I n=1 Tax=Kineobactrum sediminis TaxID=1905677 RepID=A0A2N5Y7L2_9GAMM)

HSP 1 Score: 94.7 bits (234), Expect = 2.430e-20
Identity = 48/108 (44.44%), Postives = 72/108 (66.67%), Query Frame = 1 
Query:   13 RDSLVASPGYAFVSADYSQVEMRLMAHLSSDPDLLKVFADGGDVFRTIAAALKGRGKKAADITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPEAFKIRQTVLKTY 336
            R + +A+PGY  V+ADYSQ+E+R+MAHLSSD  LL+ F DG DV +  AA + G G ++  ++ E+RR+ K + +G++YG SA  L  QL+L R EA +  +   + Y
Sbjct:  668 RQAFIAAPGYCIVAADYSQIELRIMAHLSSDEGLLRAFRDGLDVHQATAAEVFGVGLES--VSPEQRRRAKAINFGLIYGMSAFGLARQLQLGRKEAQEYIERYFERY 773
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BLAST of mRNA_Ecto-sp13_S_contig8191.19543.1 vs. uniprot
Match: A0A1G0IZ69_9GAMM (DNA polymerase I n=2 Tax=unclassified Gammaproteobacteria TaxID=33811 RepID=A0A1G0IZ69_9GAMM)

HSP 1 Score: 94.4 bits (233), Expect = 3.310e-20
Identity = 46/94 (48.94%), Postives = 63/94 (67.02%), Query Frame = 1 
Query:   13 RDSLVASPGYAFVSADYSQVEMRLMAHLSSDPDLLKVFADGGDVFRTIAAALKGRGKKAADITEEERRQTKIVVYGVLYGQSADALVPQLRLSR 294
            R + +A PGY  VSADYSQ+E+R+MAH S DP LL  F++G D+ R  AA + G   K  D+T E+RRQ K++ +G+LYG SA  L  +L  +R
Sbjct:  647 RKAFIAPPGYFIVSADYSQIELRIMAHFSQDPKLLHAFSNGTDIHRATAAEVFG--VKPEDVTPEQRRQAKVINFGILYGMSAFGLAKELGTTR 738
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig8191.19543.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FM14_ECTSI2.650e-6597.32DNA polymerase I (POL I) n=1 Tax=Ectocarpus silicu... [more]
A0A6H5L306_9PHAE9.070e-6195.54Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S3XVL9_HETAK4.490e-2449.55Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A536ZUN4_9PROT1.090e-2151.55DNA polymerase I n=1 Tax=Betaproteobacteria bacter... [more]
A0A2E6G850_9GAMM1.310e-2047.42DNA polymerase I n=1 Tax=Porticoccaceae bacterium ... [more]
A0A3B8T129_9ALTE1.760e-2045.45DNA polymerase I (Fragment) n=1 Tax=Glaciecola sp.... [more]
A0A536SNA4_9PROT1.780e-2050.52DNA polymerase I n=1 Tax=Betaproteobacteria bacter... [more]
A0A348MT94_9ALTE1.790e-2045.45DNA polymerase I n=1 Tax=Glaciecola sp. TaxID=1905... [more]
A0A2N5Y7L2_9GAMM2.430e-2044.44DNA polymerase I n=1 Tax=Kineobactrum sediminis Ta... [more]
A0A1G0IZ69_9GAMM3.310e-2048.94DNA polymerase I n=2 Tax=unclassified Gammaproteob... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig8191contigEcto-sp13_S_contig8191:4362..5938 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score214.5
Seed ortholog evalue2.5e-53
Seed eggNOG ortholog2880.D7FM14
Preferred namePOLQ
Model size337
KEGG koko:K02349
Hectar predicted targeting categoryother localisation
GOsGO:0000018,GO:0000724,GO:0000725,GO:0000726,GO:0002200,GO:0002376,GO:0002377,GO:0002440,GO:0002520,GO:0002566,GO:0003674,GO:0003676,GO:0003677,GO:0003682,GO:0003684,GO:0003824,GO:0003887,GO:0004386,GO:0004518,GO:0004527,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0006139,GO:0006259,GO:0006260,GO:0006261,GO:0006281,GO:0006282,GO:0006284,GO:0006289,GO:0006302,GO:0006303,GO:0006310,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0007275,GO:0008094,GO:0008150,GO:0008152,GO:0008409,GO:0009058,GO:0009059,GO:0009314,GO:0009416,GO:0009628,GO:0009639,GO:0009640,GO:0009653,GO:0009791,GO:0009888,GO:0009892,GO:0009933,GO:0009987,GO:0010468,GO:0010564,GO:0010569,GO:0010605,GO:0016043,GO:0016445,GO:0016446,GO:0016462,GO:0016740,GO:0016772,GO:0016779,GO:0016787,GO:0016788,GO:0016817,GO:0016818,GO:0016829,GO:0016835,GO:0016887,GO:0017111,GO:0018130,GO:0019219,GO:0019222,GO:0019438,GO:0022607,GO:0031323,GO:0031324,GO:0031974,GO:0031981,GO:0032501,GO:0032502,GO:0033554,GO:0034061,GO:0034641,GO:0034645,GO:0034654,GO:0036297,GO:0042623,GO:0042802,GO:0043142,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0045738,GO:0045910,GO:0045934,GO:0046483,GO:0048507,GO:0048519,GO:0048523,GO:0048532,GO:0048583,GO:0048585,GO:0048731,GO:0048856,GO:0050789,GO:0050793,GO:0050794,GO:0050896,GO:0051052,GO:0051053,GO:0051171,GO:0051172,GO:0051239,GO:0051259,GO:0051260,GO:0051301,GO:0051575,GO:0051716,GO:0051726,GO:0060255,GO:0065003,GO:0065007,GO:0070013,GO:0071704,GO:0071840,GO:0071897,GO:0080090,GO:0080134,GO:0080135,GO:0090304,GO:0090305,GO:0097159,GO:0097681,GO:0140097,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1901987,GO:1902749,GO:1990067,GO:2000011,GO:2000026,GO:2000042,GO:2000779,GO:2000780,GO:2001020,GO:2001021
Exons3
EggNOG free text desc.plastid DNA replication
EggNOG OGsCOG0749@1,KOG0950@2759
EC2.7.7.7
Cds size336
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03400
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464090.8437836-CDS-Ecto-sp13_S_contig8191:4361..44301681464090.8437836-CDS-Ecto-sp13_S_contig8191:4361..4430Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8191 4362..4430 +
1681464090.8590302-CDS-Ecto-sp13_S_contig8191:5269..54191681464090.8590302-CDS-Ecto-sp13_S_contig8191:5269..5419Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8191 5270..5419 +
1681464090.8700552-CDS-Ecto-sp13_S_contig8191:5821..59381681464090.8700552-CDS-Ecto-sp13_S_contig8191:5821..5938Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8191 5822..5938 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig8191.19543.1prot_Ecto-sp13_S_contig8191.19543.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig8191 4362..5938 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig8191.19543.1

>prot_Ecto-sp13_S_contig8191.19543.1 ID=prot_Ecto-sp13_S_contig8191.19543.1|Name=mRNA_Ecto-sp13_S_contig8191.19543.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=112bp
MIKPRDSLVASPGYAFVSADYSQVEMRLMAHLSSDPDLLKVFADGGDVFR
TIAAALKGRGKKAADITEEERRQTKIVVYGVLYGQSADALVPQLRLSRPE
AFKIRQTVLKTY
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mRNA from alignment at Ecto-sp13_S_contig8191:4362..5938+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig8191.19543.1 ID=mRNA_Ecto-sp13_S_contig8191.19543.1|Name=mRNA_Ecto-sp13_S_contig8191.19543.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1577bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8191:4362..5938+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGATTAAACCCAGGGACAGCCTCGTAGCGTCCCCGGGGTATGCTTTCGT
GTCCGCTGACTACTCGCAGGTGATGAAATGTGTGCGTACTAAGGGTGCCC
TATTGGTGTGAGTGTTCATCGTCCCATGCATAAACGCTTCAGACGCGCTT
CCGCTGTTGCTTGAACCTGAACATCGCAAGCACCGAAACGTCGGCGGTTA
TGATCTACATTCCCAAGCTGGAACACACCCCAGGGACTAGAATGCTGGTT
GTTACAAGCTCATTTGATGTTCGTGGGCGCCTCTGTGACAATGACCTCTA
CCTTACTTTCCGTATATTGCCTCCCGTGCACTCTATGGTTTTCGCGGAGA
CAGACACCGATGGGTTAGTCGGGGACGACGTGGTGAAATGTTTGTAGTTG
TGAGGAGAGCCATAAAAGCCGGTTTGCAACCGTACAAGCGGGTGTTGTTT
TATGGTCAACCACAGCATGATGGTATCAGGCGCGGAAGGATGTAATAATT
CTATCTTGGAAGTGTGGGCGATGGAGATGGATTGGGCTGGGATGTAAAGC
TGCATCATACGAGCGACAATCATGTTTCACGTGTAACCTCTTCGGACATC
GATGAGTTTGATGTTACTGCAGTGCCGTGTCTTTGTCTTCATGTCATGGT
AGCTGTTTGGAAGTGGGTCGATGCGGGCGGACGTTTCATCTCCTCGTAAT
GCTTTTGCGGTTAGCGTCGTCATCGTGTGTGCGGAGAATAAAAGCAGTCT
TACAACATTTCGCAGAGATCGGGTGATGGCACAGTCGGTCCTGTCCTGCC
CACCTGATACTCACAGTTCCAACACAACGCTCAGCACACAGATGTACGAC
CCTCCACCCTCGCCCGCCACCCATGTTTGCCCGCCCGTTGCAAATTGCTA
CGATGCAGGTGGAAATGCGACTGATGGCCCATCTCTCCAGCGACCCTGAT
CTTCTGAAAGTGTTTGCCGACGGGGGGGACGTGTTTCGGACGATTGCGGC
GGCGCTGAAGGGAAGGGGCAAGAAGGCCGCTGACATCACAGAAGAGGAAC
GTCGTCAGGTTCGTCCGGTCTTGGAATGAGAGGTGGTGGTTGATTTTGTC
GTGCCTCTCTTGTTGATCCGTCGAACGGTGCGTCTCTTTTCTAGCGTTTG
TGCCCGTCTTGGTACAAATCTGGTACTGCAACTAATGTGTTTCGTGTGCA
CACCCACGTATCGTACCTACCATTCTACACCCTACGTCTTTGCTCAGTTT
TAGCCAACGGTCCCCTCGTCCCCTCTCCCCCGGCCCACCCCTGAGATTTC
TTACGCAATATCAATGCGGCTACTCTGAGGCACAAAGGACGAAAGAATGC
ATGATCCATCCTCTCGAGGACGCGTGGTCAATGGTCTTACCGCTCAGACA
TGCAAAACAATACAGTACGCCCCCCACCCCCGCCTTCGACGTGTTGATCG
ACCTCTCCAGACAAAGATCGTCGTGTACGGAGTGCTGTACGGGCAGTCAG
CGGATGCCCTCGTGCCGCAGCTGCGCCTGAGCCGTCCCGAGGCCTTTAAG
ATCAGGCAGACGGTGCTCAAGACTTAC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig8191:4362..5938+

>mRNA_Ecto-sp13_S_contig8191.19543.1 ID=mRNA_Ecto-sp13_S_contig8191.19543.1|Name=mRNA_Ecto-sp13_S_contig8191.19543.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=336bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8191:4362..5938+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGATTAAACCCAGGGACAGCCTCGTAGCGTCCCCGGGGTATGCTTTCGT
GTCCGCTGACTACTCGCAGGTGGAAATGCGACTGATGGCCCATCTCTCCA
GCGACCCTGATCTTCTGAAAGTGTTTGCCGACGGGGGGGACGTGTTTCGG
ACGATTGCGGCGGCGCTGAAGGGAAGGGGCAAGAAGGCCGCTGACATCAC
AGAAGAGGAACGTCGTCAGACAAAGATCGTCGTGTACGGAGTGCTGTACG
GGCAGTCAGCGGATGCCCTCGTGCCGCAGCTGCGCCTGAGCCGTCCCGAG
GCCTTTAAGATCAGGCAGACGGTGCTCAAGACTTAC
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