mRNA_Ecto-sp13_S_contig7742.18977.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig7742.18977.1
Unique NamemRNA_Ecto-sp13_S_contig7742.18977.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig7742.18977.1 vs. uniprot
Match: A0A6H5KVE7_9PHAE (PIPK domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KVE7_9PHAE)

HSP 1 Score: 245 bits (625), Expect = 1.110e-74
Identity = 125/136 (91.91%), Postives = 128/136 (94.12%), Query Frame = 1 
Query:    1 RIPSSGDASAPAGAAASREAQEGRGDAGAATGAGGNDTLRQNGAAQDRDEPSPVGPGGESVVRYRADGGVKSEVEGGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLYYERFVDFLDHHTE 408
            RIPSSGDASAPAG AASR AQEGRGDAGAAT AG +DTL QNGAAQ+ DEP PVGPGGESVVRYRADGGVKSEV+GGAPGKDIYYLGIIDILQQYNTRKH ETFWKGLTHDRYEVSCVDPKLYYERFVDFLDHHTE
Sbjct:  240 RIPSSGDASAPAGTAASRGAQEGRGDAGAATAAGRDDTL-QNGAAQNEDEPPPVGPGGESVVRYRADGGVKSEVDGGAPGKDIYYLGIIDILQQYNTRKHVETFWKGLTHDRYEVSCVDPKLYYERFVDFLDHHTE 374
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BLAST of mRNA_Ecto-sp13_S_contig7742.18977.1 vs. uniprot
Match: D8LLT5_ECTSI (PIPK domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LLT5_ECTSI)

HSP 1 Score: 206 bits (525), Expect = 2.490e-57
Identity = 109/137 (79.56%), Postives = 112/137 (81.75%), Query Frame = 1 
Query:    1 RIPSSGDASAPAGAAASREAQEGRGDAGAATGAGGNDTLRQNGAAQDRDEPS-PVGPGGESVVRYRADGGVKSEVEGGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLYYERFVDFLDHHTE 408
            RIPSSGDASAPAGA ASREAQEGRGDAGAAT                      P+GPGGESVVRYRADGG+KSEV+GGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLY ERFVDFLDHH E
Sbjct:  496 RIPSSGDASAPAGATASREAQEGRGDAGAATAXXXXXXXXXXXXXXXXXXXXXPLGPGGESVVRYRADGGIKSEVDGGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLYCERFVDFLDHHME 632
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BLAST of mRNA_Ecto-sp13_S_contig7742.18977.1 vs. uniprot
Match: D8LKC9_ECTSI (PIPK domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LKC9_ECTSI)

HSP 1 Score: 105 bits (263), Expect = 4.460e-21
Identity = 47/76 (61.84%), Postives = 58/76 (76.32%), Query Frame = 1 
Query:  181 VVRYRADGGVKSEVEGGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLYYERFVDFLDHHTE 408
             ++ R DGG+ SEV GG  G+DIY++GIIDILQ+YN RKH ETF K L HDR E+SCVDP  Y +RF DFL+ HT+
Sbjct: 1044 AIKRRLDGGLDSEV-GGKRGRDIYFMGIIDILQEYNARKHGETFLKSLAHDRAEISCVDPATYCQRFNDFLERHTD 1118
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BLAST of mRNA_Ecto-sp13_S_contig7742.18977.1 vs. uniprot
Match: A0A2D4CAQ2_PYTIN (Phosphatidylinositol-4-phosphate 5 kinase-like protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4CAQ2_PYTIN)

HSP 1 Score: 81.6 bits (200), Expect = 2.980e-15
Identity = 37/80 (46.25%), Postives = 51/80 (63.75%), Query Frame = 1 
Query:  166 PGGESVVRYRADGGVKSEVEGGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLYYERFVDFLDHHT 405
            PG    V  + +GG+      G     +Y+LG+IDILQQYNTRK AETF KG  H+R E+S V+P  Y +RF++F++ H 
Sbjct:   15 PGHSQSVFCKDEGGIYGRDRFGRKNGFVYFLGVIDILQQYNTRKIAETFIKGFRHNRKEISAVNPVFYGDRFIEFMEKHV 94
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BLAST of mRNA_Ecto-sp13_S_contig7742.18977.1 vs. uniprot
Match: A0A6H5JMY7_9PHAE (PIPK domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JMY7_9PHAE)

HSP 1 Score: 87.4 bits (215), Expect = 3.690e-15
Identity = 46/106 (43.40%), Postives = 62/106 (58.49%), Query Frame = 1 
Query:  109 DTLRQNGAAQDRDEPSPVGPGG-------ESVVRYRADGGVKSEVEGGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLYYERFVDFLDHHT 405
            +  R   A  D D  S VG G           +R+R DGG+ SE + G   K+IY+LGIID+LQ Y   KHAET++K +   + E+SCVDP LY ERF+ F++  T
Sbjct:  394 EAFRAAAADTDGDGASTVGTGTTKPSAGYSGAIRFRPDGGIDSESDSGP--KEIYWLGIIDVLQVYTNMKHAETWYKAIGKAKNELSCVDPVLYCERFIGFMEEST 497
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BLAST of mRNA_Ecto-sp13_S_contig7742.18977.1 vs. uniprot
Match: A0A7S2S142_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2S142_9STRA)

HSP 1 Score: 84.7 bits (208), Expect = 4.490e-15
Identity = 39/76 (51.32%), Postives = 46/76 (60.53%), Query Frame = 1 
Query:  181 VVRYRADGGVKSEVEGGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLYYERFVDFLDHHTE 408
             +  R D G+ S    G  G +IY+ GIIDILQQYN  K AETF KG+ HD  ++SCV P  Y ERF  FL  H E
Sbjct:  191 ALTNRRDFGIGSRTPEGTKGNEIYFFGIIDILQQYNVSKQAETFVKGIRHDTSQISCVPPGRYAERFTKFLAEHVE 266
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BLAST of mRNA_Ecto-sp13_S_contig7742.18977.1 vs. uniprot
Match: A0A4D9DCS9_9STRA (PIPK domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9DCS9_9STRA)

HSP 1 Score: 85.1 bits (209), Expect = 2.950e-14
Identity = 36/78 (46.15%), Postives = 52/78 (66.67%), Query Frame = 1 
Query:  175 ESVVRYRADGGVKSEVEGGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLYYERFVDFLDHHTE 408
            E  + +R D GV+S +  G  G ++Y++G+IDILQQYN  K AETF+K   +D  ++S VDPK Y  RFV+F  +H +
Sbjct:  644 ERRLTHRRDHGVQSRLADGTKGNEVYFVGVIDILQQYNALKRAETFFKSFKYDSRQISAVDPKWYARRFVEFTSNHVQ 721
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BLAST of mRNA_Ecto-sp13_S_contig7742.18977.1 vs. uniprot
Match: A0A7S2CFA7_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2CFA7_9STRA)

HSP 1 Score: 78.6 bits (192), Expect = 3.490e-14
Identity = 37/72 (51.39%), Postives = 44/72 (61.11%), Query Frame = 1 
Query:  193 RADGGVKSEVEGGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLYYERFVDFLDHHTE 408
            R D GV      G  G  IY+ GIIDILQ+YNT K AET++KG +HD   +SCV P  Y  RF +FL  H E
Sbjct:   52 REDYGVNERDANGHKGNGIYFFGIIDILQEYNTAKKAETWFKGFSHDTNLISCVGPDRYARRFTEFLTAHIE 123
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BLAST of mRNA_Ecto-sp13_S_contig7742.18977.1 vs. uniprot
Match: A0A7H8REC7_9EURO (PIPK domain-containing protein n=2 Tax=Talaromyces sect. Islandici TaxID=2752542 RepID=A0A7H8REC7_9EURO)

HSP 1 Score: 84.7 bits (208), Expect = 4.340e-14
Identity = 36/73 (49.32%), Postives = 52/73 (71.23%), Query Frame = 1 
Query:  178 SVVRYRADGGVKSEVEGGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLYYERFVDFLD 396
            ++V Y  DGG+++  E G PG++IYYLGIID L  Y   K AE FWKGL+H+R ++S + P+ Y ERF+ F++
Sbjct:  688 NLVFYSDDGGLRATHENGQPGEEIYYLGIIDCLTPYGMVKKAEHFWKGLSHNRSQISPIPPQQYGERFIQFME 760
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BLAST of mRNA_Ecto-sp13_S_contig7742.18977.1 vs. uniprot
Match: A0A0F4Z4L1_TALEM (1-phosphatidylinositol-4-phosphate 5-kinase n=1 Tax=Rasamsonia emersonii CBS 393.64 TaxID=1408163 RepID=A0A0F4Z4L1_TALEM)

HSP 1 Score: 84.3 bits (207), Expect = 5.810e-14
Identity = 35/73 (47.95%), Postives = 50/73 (68.49%), Query Frame = 1 
Query:  178 SVVRYRADGGVKSEVEGGAPGKDIYYLGIIDILQQYNTRKHAETFWKGLTHDRYEVSCVDPKLYYERFVDFLD 396
            ++V Y  DGG ++  E G PG++IYYLGIID L  Y   K  E FWKGL+H+R ++S + P  Y ERF++F++
Sbjct:  684 NLVFYSDDGGFRATHENGQPGEEIYYLGIIDCLTHYGVLKKMENFWKGLSHNRSQISPIPPDAYGERFIEFME 756
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig7742.18977.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KVE7_9PHAE1.110e-7491.91PIPK domain-containing protein n=1 Tax=Ectocarpus ... [more]
D8LLT5_ECTSI2.490e-5779.56PIPK domain-containing protein n=1 Tax=Ectocarpus ... [more]
D8LKC9_ECTSI4.460e-2161.84PIPK domain-containing protein n=1 Tax=Ectocarpus ... [more]
A0A2D4CAQ2_PYTIN2.980e-1546.25Phosphatidylinositol-4-phosphate 5 kinase-like pro... [more]
A0A6H5JMY7_9PHAE3.690e-1543.40PIPK domain-containing protein n=2 Tax=Ectocarpus ... [more]
A0A7S2S142_9STRA4.490e-1551.32Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A4D9DCS9_9STRA2.950e-1446.15PIPK domain-containing protein n=1 Tax=Nannochloro... [more]
A0A7S2CFA7_9STRA3.490e-1451.39Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A7H8REC7_9EURO4.340e-1449.32PIPK domain-containing protein n=2 Tax=Talaromyces... [more]
A0A0F4Z4L1_TALEM5.810e-1447.951-phosphatidylinositol-4-phosphate 5-kinase n=1 Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig7742contigEcto-sp13_S_contig7742:849..3438 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score208.4
Seed ortholog evalue2.2e-51
Seed eggNOG ortholog2880.D8LLT5
Model size1122
KEGG rclassRC00002,RC00078
KEGG koko:K00889
KEGG ReactionR03469
KEGG Pathwayko00562,ko01100,ko04011,ko04070,ko04072,ko04139,ko04144,ko04666,ko04810,ko05231,map00562,map01100,map04011,map04070,map04072,map04139,map04144,map04666,map04810,map05231
KEGG ModuleM00130
Hectar predicted targeting categoryother localisation
Exons3
EggNOG free text desc.Translation initiation factor IF-2, N-terminal region
EggNOG OGsCOG5253@1,KOG0229@2759
EC2.7.1.68
Cds size411
COG Functional cat.T
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko04131
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464044.3482666-UTR-Ecto-sp13_S_contig7742:848..15591681464044.3482666-UTR-Ecto-sp13_S_contig7742:848..1559Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig7742 849..1559 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464044.3653615-CDS-Ecto-sp13_S_contig7742:1559..16911681464044.3653615-CDS-Ecto-sp13_S_contig7742:1559..1691Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7742 1560..1691 -
1681464044.3801503-CDS-Ecto-sp13_S_contig7742:2508..26711681464044.3801503-CDS-Ecto-sp13_S_contig7742:2508..2671Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7742 2509..2671 -
1681464044.3929095-CDS-Ecto-sp13_S_contig7742:3322..34381681464044.3929095-CDS-Ecto-sp13_S_contig7742:3322..3438Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7742 3323..3438 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig7742.18977.1prot_Ecto-sp13_S_contig7742.18977.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig7742 1560..3438 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig7742.18977.1

>prot_Ecto-sp13_S_contig7742.18977.1 ID=prot_Ecto-sp13_S_contig7742.18977.1|Name=mRNA_Ecto-sp13_S_contig7742.18977.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=137bp
RIPSSGDASAPAGAAASREAQEGRGDAGAATGAGGNDTLRQNGAAQDRDE
PSPVGPGGESVVRYRADGGVKSEVEGGAPGKDIYYLGIIDILQQYNTRKH
AETFWKGLTHDRYEVSCVDPKLYYERFVDFLDHHTE*
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mRNA from alignment at Ecto-sp13_S_contig7742:849..3438-

Legend: polypeptideCDSUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig7742.18977.1 ID=mRNA_Ecto-sp13_S_contig7742.18977.1|Name=mRNA_Ecto-sp13_S_contig7742.18977.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2590bp|location=Sequence derived from alignment at Ecto-sp13_S_contig7742:849..3438- (Ectocarpus species13 EcNAP12_S_4_19m)
AGGATTCCGAGTTCGGGGGATGCTTCGGCTCCCGCTGGGGCTGCTGCTTC
GAGAGAGGCGCAGGAGGGGAGGGGGGATGCGGGGGCGGCGACGGGCGCTG
GAGGAAACGATACGTTGTGAGTGCCGTGGTGCCGGTGCTACGGTTGGTGG
TGCTGTTGATGATGACGTTGTTTCTCCTGGCATGTGGATACGATTCCAAG
CAGGTGATGGCAGCGGATGGGATATTTGGTAACCAGGAGGCCAGGGGGGC
GAGTGTGTCAAACATTTTCTCTCCCACGTGCTGTAACTATTCGAGACAAA
TTAGGCCGGGCGCATCAGTTGGTTTGAGTATGAAAGGGTGTAGCTGCAGG
ATTTCCAAGTTTCTCTCTCATGTAAGCACGTAAGCACGCTCCACCCGGCC
CGAGCAGGTTTTTGTTTGCGGCCACTCATCTTTCACTTGCTACGCTTTAG
CATACGTTCACATCTTTTTTATGCTCGGGTTGTCGAGATAGCCAAAGAGG
CAAGGCAAGCGACGAAGTGTTTTGATAAAACGCCCGGTGCTGGACGTTAT
CGGTCCCTGGTTGGCGGCTAGCTCTACACCAAGACTTGAGAGGCTCACCT
CCAGCAATGATAGGCCTACCTTAGACATGCACAAGTTACACCTACGGTGC
CCCCCCCGCCTCCTTTAACCCGGGAAAAGCGCACCTACAGGAGTGGTAGG
CTTACCTTGAGACATCTACTAGTTAAACATGTAAGCTGTGTTTCTTTTCG
CTGACCCTCCCCGAAAGGAGACAAAACGGGGCAGCGCAAGATAGGGACGA
ACCCTCCCCGGTGGGGCCTGGGGGAGAGTCTGTCGTCCGGTACCGAGCAG
ACGGAGGTGTCAAGTCCGAGGTTGAAGGGGGGGCTCCTGGAAAGGACATC
TACTATCTGGGCATCATCGACATCTTGCAGGTATACAACACGAACAATAG
TTATGATACTCCACAGAACAGCGGCAGCGGCGTATGACGCGCTTCAGCAA
ACGTGATGTTTGCAGAATGCCTCGGCACAGATTGAATGGTCTCTGCTCGG
CCGGTGTGTGGTAGAGTAGGAGTTCAGAGCAAGATTACAGGCGTGCTTAC
GGTAGGGCAATCCCCCCCCCCGTGCTTGTCTCCGTCGTCGTGCTGTGGCT
GCGAGGAGTCTCGATGTTCCGCACTGATGAGCCAAAATACGAAAGTGAAG
AAAATGAATGACTTTCTTTGCGTTCCTCAGGAGCAGCAGCTCCTCCAGCT
CGTCCGTGTTCATTTGTTGTTGCAGTTGAACGAGAACATGAAGAGTGTTG
TAGATGGCATATTTGGAACATTATCGAGAGTCTTGCGAGTGAGGTTTTGC
AGCCCACTCTGTGCTACAGATACGTGGCTCTCGAGGGCCGAGCAGTTGTC
CACCCAGAGGAGCAAGTACTCCACGATGTTACGTGAACATGCGCGATTCA
CTGTGGTAGCACGGATGACGTGTTCGCGGTTTGCTGGAGTGGTCGCGCTG
TGGCTGCTGCGGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCT
GCTGCTGTTATTGTCGTTGGTCGTGTCGGTCGGGAGTCCCCCCCCCCCCC
CCCCCCCCGCCTCCCTCCGCCAACACTCCCCCCCCCCGTGCCCCCGTGTT
TTTTTTTGGGTGGTGCGGGCGGGGGGCCCCCCCCCCCCCCCCGGGCGATT
CCCAATCGTCTACCCCCTCCCGCTCCGTGTCCCCCGTGCGGCGGCAGCAA
TACAACACTCGCAAGCACGCCGAGACGTTCTGGAAAGGGCTGACCCACGA
CCGCTATGAGGTATCCTGCGTCGACCCGAAGCTCTACTACGAGCGATTCG
TCGATTTCTTGGACCACCACACGGAATAGACTCGTGAACACTTTTAGTAA
CAACACCGCGGACAATAATAACGAGTAATTCTAGCAAGCGGTTGTTGCTT
GTTTGGTTCACGTATTTATTTAGTTACACAGCAGTCAACCAGCTTGCTTT
TGCGTGGATTGTGGCCGGTTGAGGAGCGTAATACTACTTTCTGCTGGTGT
CCTGTGTGATCGATCTTCTTGTGACTGTTCTCGTCTTCGGCGTCCTCAAG
TTTCTGGTATCAGTAAAATAAAGCTTCTGGTCGCCGGTTCTGGCGTCTAC
TCGGCGCCCTCTTGAGCACTGGTGGCCACACTTGGCGTGTTACAACGTAG
CATTACCTTATGTACCACGAGTACGTGTGTACTTCAATTCAACACTGCTG
CCAACAATGTTCCGTGGTGGACAGATGCATGGACCTATGATCCGCACCGT
TTCATTTTTTTGCTGGATACATCTGCCACGCGTGCGTGCGGATGTGGCGT
TCTTCTAAATTTGCGTGTACTGTGTGTCATCCCAACATGATGTGATATCT
GTTGCGTGGCTATGTGTGGTGGTTGCTGTTTGTCGCGTGGATTGCGTGGC
CGAGTAAAGCTTGTAGTGATGCCTTGATACGTACCGCTGCTACATAACAA
GCAGCTGTGTTGTAGTTGCATTTGCCAGCGAGCGAGCGACTCTCCAACAT
TTTGGACAAAACCGCGGGACGTAATTGAAACGAACTCTTG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig7742:849..3438-

>mRNA_Ecto-sp13_S_contig7742.18977.1 ID=mRNA_Ecto-sp13_S_contig7742.18977.1|Name=mRNA_Ecto-sp13_S_contig7742.18977.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=411bp|location=Sequence derived from alignment at Ecto-sp13_S_contig7742:849..3438- (Ectocarpus species13 EcNAP12_S_4_19m)
AGGATTCCGAGTTCGGGGGATGCTTCGGCTCCCGCTGGGGCTGCTGCTTC
GAGAGAGGCGCAGGAGGGGAGGGGGGATGCGGGGGCGGCGACGGGCGCTG
GAGGAAACGATACGTTGAGACAAAACGGGGCAGCGCAAGATAGGGACGAA
CCCTCCCCGGTGGGGCCTGGGGGAGAGTCTGTCGTCCGGTACCGAGCAGA
CGGAGGTGTCAAGTCCGAGGTTGAAGGGGGGGCTCCTGGAAAGGACATCT
ACTATCTGGGCATCATCGACATCTTGCAGCAATACAACACTCGCAAGCAC
GCCGAGACGTTCTGGAAAGGGCTGACCCACGACCGCTATGAGGTATCCTG
CGTCGACCCGAAGCTCTACTACGAGCGATTCGTCGATTTCTTGGACCACC
ACACGGAATAG
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