mRNA_Ecto-sp13_S_contig7691.18924.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_Ecto-sp13_S_contig7691.18924.1
Unique Name	mRNA_Ecto-sp13_S_contig7691.18924.1
Type	mRNA
Organism	Ectocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)

Homology

[Close]

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot
Match: A0A6H5K474_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K474_9PHAE)

HSP 1 Score: 122 bits (305), Expect = 2.500e-26
Identity = 60/107 (56.07%), Postives = 76/107 (71.03%), Query Frame = 1

Query:  709 VGGLPSVSNHCQGSYRPFVRCAADPDIARPYPKLALLVYQETNSLLTSKEIDPLDLAELFGTVGGFWDLLLILWPIFFVASSRQAPHLKGRSFDKAVTKGMGGIRNG 1029
            +G  P V+N         V+   +P I +PY +L+L V QE NSL    E+DPLDLAE+FG VGGFWDLL+I+WPIFFVA+SRQ PHLK R+F K+V KG+GG+R G
Sbjct:  260 LGQEPIVNNKALSVSTATVKDEVNPAIVKPYAQLSLQVQQEPNSLEVITEVDPLDLAEMFGNVGGFWDLLMIMWPIFFVAASRQDPHLKPRNFKKSVNKGLGGLRVG 366

[Close]

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot
Match: D8LMD1_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LMD1_ECTSI)

HSP 1 Score: 121 bits (304), Expect = 5.200e-26
Identity = 60/107 (56.07%), Postives = 76/107 (71.03%), Query Frame = 1

Query:  709 VGGLPSVSNHCQGSYRPFVRCAADPDIARPYPKLALLVYQETNSLLTSKEIDPLDLAELFGTVGGFWDLLLILWPIFFVASSRQAPHLKGRSFDKAVTKGMGGIRNG 1029
            +G  P V+N         V+   +P I +PY +L+L   QE NSL    E+DPLDLAE+FG VGGFWDLL+I+WPIFFVA+SRQ PHLK R+F K+VTKG+GG+R G
Sbjct:  292 LGQEPIVNNKALSVSTATVKDEVNPAIVKPYAQLSLQFQQEPNSLEIITEVDPLDLAEMFGNVGGFWDLLMIMWPIFFVAASRQDPHLKPRNFTKSVTKGLGGLRIG 398

[Close]

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot
Match: A0A6H5JN97_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JN97_9PHAE)

HSP 1 Score: 119 bits (297), Expect = 1.560e-25
Identity = 64/137 (46.72%), Postives = 85/137 (62.04%), Query Frame = 1

Query:  703 MSVGGLPSVSNHCQGSYRPFVRCAADPDIARPYPKLALLVYQETNSLLTSKEIDPLDLAELFGTVGGFWDLLLILWPIFFVASSRQAPHLKGRSFDKAVTKGMGGIRNGSPVEQVDPS*RSGSGSWSARANTGRDGR 1113
            + +G  P VSN         V+   +P I +PY +L+L V Q  NS     E+DPLDLAE+FG VGGFWDLL+I+WPIFFVA+SRQ PHLK R+F ++VTKG+ G+R G           +GS S   R N  ++G+
Sbjct:  218 IKLGQEPIVSNKALSVSTATVKHEVNPAIVKPYAQLSLQVQQAPNSHECITEVDPLDLAEMFGNVGGFWDLLMIIWPIFFVAASRQDPHLKPRNFKESVTKGLSGLRIGDSKHPAARDGFNGSPSVEERPNWEQEGK 354

[Close]

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot
Match: A0A6H5K6D1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K6D1_9PHAE)

HSP 1 Score: 107 bits (266), Expect = 1.010e-21
Identity = 51/84 (60.71%), Postives = 63/84 (75.00%), Query Frame = 1

Query:  787 IARPYPKLALLVYQETNSLLTSKEIDPLDLAELFGTVGGFWDLLLILWPIFFVASSRQAPHLKGRSFDKAVTKGMGGIRNGSPV 1038
            I +PY +L+L   QE NSL    E DP DLAE+FG VGGFWDLL+++WPIFFVA+SRQ PHLK R+F K+V KG+ G+R G  V
Sbjct:  204 IDKPYAQLSLQFQQEPNSLEVITENDPFDLAEMFGNVGGFWDLLMLVWPIFFVAASRQDPHLKPRNFKKSVAKGLQGLRVGEVV 287

[Close]

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot
Match: D8LMD2_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LMD2_ECTSI)

HSP 1 Score: 106 bits (265), Expect = 2.610e-21
Identity = 51/84 (60.71%), Postives = 63/84 (75.00%), Query Frame = 1

Query:  787 IARPYPKLALLVYQETNSLLTSKEIDPLDLAELFGTVGGFWDLLLILWPIFFVASSRQAPHLKGRSFDKAVTKGMGGIRNGSPV 1038
            I +PY +L+L   QE NSL    E DP DLAE+FG VGGFWDLL+++WPIFFVA+SRQ PHLK R+F K+V KG+ G+R G  V
Sbjct:  247 IDKPYAQLSLQFQQEPNSLEVITENDPFDLAEMFGNVGGFWDLLMLVWPIFFVAASRQDPHLKPRNFKKSVAKGLEGLRIGELV 330

[Close]

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot
Match: D7FU22_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FU22_ECTSI)

HSP 1 Score: 103 bits (256), Expect = 8.290e-21
Identity = 47/74 (63.51%), Postives = 61/74 (82.43%), Query Frame = 1

Query:  787 IARPYPKLALLVYQETNSLLTSKEIDPLDLAELFGTVGGFWDLLLILWPIFFVASSRQAPHLKGRSFDKAVTKG 1008
            IA PY  L++ + Q T+SL    EI+PL++AELFG VGGFWDLLL+LWPIFF+++SRQ PHLKGR+F K+VT+G
Sbjct:   52 IASPYAWLSMEIAQSTDSLEEITEINPLEIAELFGNVGGFWDLLLLLWPIFFISASRQDPHLKGRNFKKSVTRG 125

[Close]

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot
Match: D7FU23_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FU23_ECTSI)

HSP 1 Score: 99.0 bits (245), Expect = 2.630e-18
Identity = 46/74 (62.16%), Postives = 60/74 (81.08%), Query Frame = 1

Query:  787 IARPYPKLALLVYQETNSLLTSKEIDPLDLAELFGTVGGFWDLLLILWPIFFVASSRQAPHLKGRSFDKAVTKG 1008
            IA PY  L++ + Q T+SL    EI+PL++AELFG VGGFWDLLL+LWPIFF+++SRQ PHLK R+F K+VT+G
Sbjct:  245 IASPYAWLSMEIAQTTDSLEEITEINPLEIAELFGNVGGFWDLLLMLWPIFFISASRQDPHLKVRNFKKSVTRG 318

[Close]

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot
Match: A0A6H5JF84_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JF84_9PHAE)

HSP 1 Score: 86.3 bits (212), Expect = 1.960e-15
Identity = 38/77 (49.35%), Postives = 56/77 (72.73%), Query Frame = 1

Query:  775 ADPDIARPYPKLALLVYQETNSLLTSKEIDPLDLAELFGTVGGFWDLLLILWPIFFVASSRQAPHLKGRSFDKAVTK 1005
            AD DI +PY +L++ + Q+ +S     E DP + AE+ G +GGFWDL+LILWP+FFVA+S+  P+LK R+F K+ T+
Sbjct:   79 ADGDITKPYARLSMELKQQFDSWEIITEADPFEFAEILGNIGGFWDLILILWPLFFVAASQPEPNLKPRNFAKSFTR 155

[Close]

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot
Match: D7FXH8_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXH8_ECTSI)

HSP 1 Score: 88.6 bits (218), Expect = 3.890e-15
Identity = 39/77 (50.65%), Postives = 57/77 (74.03%), Query Frame = 1

Query:  775 ADPDIARPYPKLALLVYQETNSLLTSKEIDPLDLAELFGTVGGFWDLLLILWPIFFVASSRQAPHLKGRSFDKAVTK 1005
            AD DI +PY +L++ + Q+ +S     E DP + AE+ G +GGFWDL+LILWP+FFVA+S+  P+LK R+F K+VT+
Sbjct:  257 ADADITKPYARLSMELKQQFDSWEIITEADPFEFAEILGNIGGFWDLILILWPLFFVAASQPEPNLKPRNFAKSVTR 333

[Close]

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot
Match: D8LNQ1_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNQ1_ECTSI)

HSP 1 Score: 86.3 bits (212), Expect = 1.210e-14
Identity = 39/77 (50.65%), Postives = 56/77 (72.73%), Query Frame = 1

Query:  778 DPDIARPYPKLALLVYQETNSLLTSKEIDPLDLAELFGTVGGFWDLLLILWPIFFVASSRQAPHLKGRSFDKAVTKG 1008
            +P+I  PY  L+L + Q+ +S     E +P + AE+FG +GGFWDLLLILWP+ FVA+++Q PHLK R+F K+V +G
Sbjct:  198 NPNITMPYAYLSLEMKQQADSWEIITESNPYEFAEMFGNIGGFWDLLLILWPLCFVATTQQDPHLKPRTFKKSVVRG 274

The following BLAST results are available for this feature:

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot

BLAST of mRNA_Ecto-sp13_S_contig7691.18924.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 20


Match Name	E-value	Identity	Description
A0A6H5K474_9PHAE	2.500e-26	56.07	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]
D8LMD1_ECTSI	5.200e-26	56.07	Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2...	[more]
A0A6H5JN97_9PHAE	1.560e-25	46.72	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]
A0A6H5K6D1_9PHAE	1.010e-21	60.71	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]
D8LMD2_ECTSI	2.610e-21	60.71	Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2...	[more]
D7FU22_ECTSI	8.290e-21	63.51	Uncharacterized protein n=1 Tax=Ectocarpus silicul...	[more]
D7FU23_ECTSI	2.630e-18	62.16	Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2...	[more]
A0A6H5JF84_9PHAE	1.960e-15	49.35	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]
D7FXH8_ECTSI	3.890e-15	50.65	Uncharacterized protein n=1 Tax=Ectocarpus silicul...	[more]
D8LNQ1_ECTSI	1.210e-14	50.65	Uncharacterized protein n=1 Tax=Ectocarpus silicul...	[more]

Pages

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
Ecto-sp13_S_contig7691	contig	Ecto-sp13_S_contig7691:3682..6400 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 vs UniRef90	2022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.0	2022-07-08

Properties


Property Name	Value
Stop	1
Start	1
Model size	1766
Hectar predicted targeting category	signal peptide
Exons	3
Cds size	456

Relationships

The following UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1681464039.710066-UTR-Ecto-sp13_S_contig7691:3681..4284	1681464039.710066-UTR-Ecto-sp13_S_contig7691:3681..4284	Ectocarpus species13 EcNAP12_S_4_19m	UTR	Ecto-sp13_S_contig7691 3682..4284 +
1681464039.7425447-UTR-Ecto-sp13_S_contig7691:5041..5048	1681464039.7425447-UTR-Ecto-sp13_S_contig7691:5041..5048	Ectocarpus species13 EcNAP12_S_4_19m	UTR	Ecto-sp13_S_contig7691 5042..5048 +
1681464039.7539518-UTR-Ecto-sp13_S_contig7691:5700..6400	1681464039.7539518-UTR-Ecto-sp13_S_contig7691:5700..6400	Ectocarpus species13 EcNAP12_S_4_19m	UTR	Ecto-sp13_S_contig7691 5701..6400 +

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1681464039.7220159-CDS-Ecto-sp13_S_contig7691:4284..4591	1681464039.7220159-CDS-Ecto-sp13_S_contig7691:4284..4591	Ectocarpus species13 EcNAP12_S_4_19m	CDS	Ecto-sp13_S_contig7691 4285..4591 +
1681464039.7322354-CDS-Ecto-sp13_S_contig7691:4892..5041	1681464039.7322354-CDS-Ecto-sp13_S_contig7691:4892..5041	Ectocarpus species13 EcNAP12_S_4_19m	CDS	Ecto-sp13_S_contig7691 4893..5041 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_Ecto-sp13_S_contig7691.18924.1	prot_Ecto-sp13_S_contig7691.18924.1	Ectocarpus species13 EcNAP12_S_4_19m	polypeptide	Ecto-sp13_S_contig7691 4285..5041 +

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at Ecto-sp13_S_contig7691:3682..6400+
coding sequence from alignment at Ecto-sp13_S_contig7691:3682..6400+

protein sequence of mRNA_Ecto-sp13_S_contig7691.18924.1

>prot_Ecto-sp13_S_contig7691.18924.1 ID=prot_Ecto-sp13_S_contig7691.18924.1|Name=mRNA_Ecto-sp13_S_contig7691.18924.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=152bp
MCRTFATLFLFTIVLLYFAENYDVSCAIRPFCVMSVGGLPSVSNHCQGSY
RPFVRCAADPDIARPYPKLALLVYQETNSLLTSKEIDPLDLAELFGTVGG
FWDLLLILWPIFFVASSRQAPHLKGRSFDKAVTKGMGGIRNGSPVEQVDP
S*

mRNA from alignment at Ecto-sp13_S_contig7691:3682..6400+

Legend: UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_Ecto-sp13_S_contig7691.18924.1 ID=mRNA_Ecto-sp13_S_contig7691.18924.1|Name=mRNA_Ecto-sp13_S_contig7691.18924.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2719bp|location=Sequence derived from alignment at Ecto-sp13_S_contig7691:3682..6400+ (Ectocarpus species13 EcNAP12_S_4_19m)
CTCCCCTCCTCCTTTCCGAGCACGACAGCGTCCTGGGTACGGGCGTTGTC
TCACCGTCTGAAGTTAATACACTACACAAACAAGTCCCGACAAGTACGCG
GAGTACGGCCGACAAACAGTGGATATACATCACCTTCAAGGACGCAGGAA
CAGGGAGCCTCTCCTCCGAAATCCCACTTCCGTATACCTGCATTTCTAAT
TGGTCAACCAGTCGGGAGTTCACGCACGTGGTTAGTTGCGTTCTCCTTTT
TGCTTTCCGATGAATACACACGATTTTGGCCGGTAGTTGAAAACTTTGTT
CCATGGTGAAGAAGGTATGGATGCGGGTCGCGAGAATATGTGGCGCTCGA
GCGTGATGCCGTAACCGCAGAAATATTTCGAGTATAGCGCAGTTGGACAC
CGTGTGCAACGGTGCAAGGGTGGTTACATGCGACCGGTGAGATACGACAC
GCACGGTAGCGATCTACCACGAGTGCGGTGCAGCAGAAAAAGGTATCGGT
TTCAAACCTTTTACACGAATGTTGGAATGCGCCGACATAATGGTCAACGG
TAACTGGTGTACCACGTGGTATGCTTTCGTAGCAGTGGAGCGTACGTTTA
TCAATGTGTCGTACCTTTGCAACCCTTTTTCTCTTTACAATAGTTTTATT
GTACTTCGCTGAAAATTATGATGTTTCGTGTGCCATCCGACCATTTTGCG
TCATGTCGGTTGGCGGACTTCCATCGGTGTCGAACCATTGCCAAGGCTCG
TACCGTCCCTTCGTACGTTGCGCAGCCGACCCAGATATCGCGAGACCGTA
TCCCAAGCTGGCTTTGCTAGTCTACCAGGAGACGAACTCATTGCTCACCA
GCAAAGAGATCGACCCTCTCGATCTCGCCGAACTGTTTGGCACCGTCGGC
GGATTCTGGGGTGAGGACGCCGGGCGTATTATTTTTGCGTTTTGGAGGGG
AGCACGATGCAAGATAGATCGGCCAGGCGGGGAGACGAAAAGGGAATGGA
GGCGGACTATTATATGGCAAATCTTGCCACATGGTCTCGTCGAAAATTGT
TTCTGTGTCATGTCTTTGTGAGCAACCGACTTATCCATCTCTTTAACGAG
GGTAGTTTAATCCCCTACATGCACGAAAAGCATATGCCGCCTACGGTACT
GCGATGCACATGAAACTGATACATTATGTCACTGCGAAACATCGTTGACC
TTGAACCGCAGACCTGCTGCTGATATTATGGCCGATCTTCTTCGTGGCTT
CCAGCAGACAAGCTCCTCACCTTAAGGGTCGCAGTTTTGATAAGGCGGTA
ACCAAGGGGATGGGAGGTATTCGCAATGGCAGCCCAGTCGAACAAGTAGA
CCCATCATAGAGATCAGGTGAGTGAGGTGTTCCCACGCCGAGCGATAGGT
CTCAATGTTTTTCGTTCTTACCACCCGGGGGAGTGGGCAAAGGAAGGAAT
GTAAGCAGCAGTTACCTGTGCAATACATATTTTCGCCACTTCTTGCACGT
TTGGCTGATATTCATGCGACAGCTCCACCTCGCTTCGCATGCGTTGCTCC
GAAGCAAACACGCTCTTCGTCGGTGCCGCTCCTGCTAAGCGCTATGACGT
GCTGTTGTGTGAAGCAGCAGAAGCATTGTAGCAGGTTCTTCGCCGTGTCA
ACCTTGCCGAAAATTCGGCAGGAAAGGCGTCAGTCGTTGGCGCCGGTGAT
GGGCCTGACACTCGAGTGCATCCTCACCTGACATGGAGGGCGAGTGGCGT
CCCATCCCCCCAGCATCATCCCGCGCCGCCCCTAGTTACAACAGAGAATT
ACTGAGGTTCTTAGCCAGCCCAGTCGTGCCGAAGTACCGTGGCATGAATT
CGAAGGGCGACACGCTCGCCCTCTAACGGTTTCAATGGCGATAAAGTGAC
CGGGAAATGCTCCGTCGCCAGCCACACGTTCGTGTAGCACTGTCTGTTTC
GGCGGCGATCGAAGGTTCAGTTTGGCTCGACTGACGCATTTTTGTCGTGC
TCGCGGAAACTTTAAACAGGCTCTGGCAGCTGGAGCGCCCGGGCGAACAC
GGGGCGAGACGGACGGCTGCGACATTGAAGAGACGTGTCTCGCTCGCTGT
AGTATTCTGCACGGCGAGTTGGGTTTGTGAAGAATGCTTTTGTTCTGCAA
ATCCATCCCCCCGCGGGGAAATAGGGTGTGCAGGGGTAAAGGGTGGTGGC
TCTGAAGGTAACGGGAGTGCGACAAGCGTGACGTGGGAAGAAATTTGTTT
TTCGTCGAGACAAGTCTTCACCTAGGCCAGTTGTTTTCGGTGTTCGTGTA
GCCCAACGGGTAGACGAATCTCTGTCTCATTTTTCGATCTACGGTGCATG
CCTACGTGTTTTACGATCTGATGGCGAGACCTGTGCTCCGGCTGTAGAGT
ACACAATTAGCGCCAGTAGGACGAGGTAGAACTTAATTGAATTGCCTTTT
ATTCGCACGGGGGGGGATATTGTAGGGATCGCACAGGGTAGCGGTGGTCG
TTTCGTCTTTTTCCTGGCTCCAGCATCGCCGACACCCTTTCAGGGTCAAC
ATTTTCCTGTCACATCTAGGGATTTCCGCAGACCATTTGAGTTGCATCAG
GCAACTGCACTTTTTTTGCGACAGTGCTGTTGTTGGATCGCCAGCCATTA
CTGTCATGAGGTCCTGCTCCTCCTGCTGATGATTCTGACACATGTTACCT
TTTATTTACAAAAAGTCAT

Coding sequence (CDS) from alignment at Ecto-sp13_S_contig7691:3682..6400+

>mRNA_Ecto-sp13_S_contig7691.18924.1 ID=mRNA_Ecto-sp13_S_contig7691.18924.1|Name=mRNA_Ecto-sp13_S_contig7691.18924.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=456bp|location=Sequence derived from alignment at Ecto-sp13_S_contig7691:3682..6400+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGTGTCGTACCTTTGCAACCCTTTTTCTCTTTACAATAGTTTTATTGTA
CTTCGCTGAAAATTATGATGTTTCGTGTGCCATCCGACCATTTTGCGTCA
TGTCGGTTGGCGGACTTCCATCGGTGTCGAACCATTGCCAAGGCTCGTAC
CGTCCCTTCGTACGTTGCGCAGCCGACCCAGATATCGCGAGACCGTATCC
CAAGCTGGCTTTGCTAGTCTACCAGGAGACGAACTCATTGCTCACCAGCA
AAGAGATCGACCCTCTCGATCTCGCCGAACTGTTTGGCACCGTCGGCGGA
TTCTGGGACCTGCTGCTGATATTATGGCCGATCTTCTTCGTGGCTTCCAG
CAGACAAGCTCCTCACCTTAAGGGTCGCAGTTTTGATAAGGCGGTAACCA
AGGGGATGGGAGGTATTCGCAATGGCAGCCCAGTCGAACAAGTAGACCCA
TCATAG