mRNA_Ecto-sp13_S_contig7426.18606.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig7426.18606.1
Unique NamemRNA_Ecto-sp13_S_contig7426.18606.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig7426.18606.1 vs. uniprot
Match: D7FWX7_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FWX7_ECTSI)

HSP 1 Score: 309 bits (792), Expect = 9.700e-99
Identity = 149/151 (98.68%), Postives = 150/151 (99.34%), Query Frame = 1 
Query:    1 DVPCVVAGYGPGFETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVGVPSSTEYPFSYARDQHEHVIATLNDDCQVEKWDQYGDNQEQNDVFDATNAVICVIGASGLPIEECDVTPPDEPEVAQ 453
            DVPCVVAGYGPGFETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVG PSSTEYPFSYARDQHEHVIATLNDDCQVEKWDQYGDNQEQNDVFDATNAVICVIGASGLPIE+CDVTPPDEPEVAQ
Sbjct:  158 DVPCVVAGYGPGFETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVGAPSSTEYPFSYARDQHEHVIATLNDDCQVEKWDQYGDNQEQNDVFDATNAVICVIGASGLPIEDCDVTPPDEPEVAQ 308
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BLAST of mRNA_Ecto-sp13_S_contig7426.18606.1 vs. uniprot
Match: A0A6H5JJ76_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JJ76_9PHAE)

HSP 1 Score: 304 bits (778), Expect = 6.260e-98
Identity = 147/151 (97.35%), Postives = 147/151 (97.35%), Query Frame = 1 
Query:    1 DVPCVVAGYGPGFETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVGVPSSTEYPFSYARDQHEHVIATLNDDCQVEKWDQYGDNQEQNDVFDATNAVICVIGASGLPIEECDVTPPDEPEVAQ 453
            DVPCVVAGY PGFETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVG PSSTEYPFSY R QHEHVIATLNDDCQVEKWDQYGDNQEQNDVFDATNAVICVIGASGLPIEECDVTPPDEPEVAQ
Sbjct:   70 DVPCVVAGYAPGFETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVGAPSSTEYPFSYVRHQHEHVIATLNDDCQVEKWDQYGDNQEQNDVFDATNAVICVIGASGLPIEECDVTPPDEPEVAQ 220
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BLAST of mRNA_Ecto-sp13_S_contig7426.18606.1 vs. uniprot
Match: A0A6H5J7U4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J7U4_9PHAE)

HSP 1 Score: 131 bits (329), Expect = 1.070e-31
Identity = 62/119 (52.10%), Postives = 83/119 (69.75%), Query Frame = 1 
Query:    1 DVPCVVAGYGPGFETYHNENLAATDLQFTE-EFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVGVPSSTEYPFSYARDQHEHVIATLNDDCQVEKWDQYGDNQEQNDV 354
            D  C  AGY PGF+ +HNEN    DL  +E ++WT +  V+DF FD+KF  N   N+A+VRY++ VTTTDG+S+GVP++ EYPF+    QHEH +  ++DDC    WDQYGD+QEQ DV
Sbjct:   81 DSMCAAAGYDPGFQRFHNENFTG-DLPVSEPDYWTGAFAVVDFSFDIKFTNNPSPNRASVRYIEAVTTTDGLSLGVPATFEYPFNVTILQHEHALVDVDDDCNFVLWDQYGDDQEQTDV 198
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BLAST of mRNA_Ecto-sp13_S_contig7426.18606.1 vs. uniprot
Match: D7FMJ9_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FMJ9_ECTSI)

HSP 1 Score: 129 bits (323), Expect = 8.330e-31
Identity = 61/119 (51.26%), Postives = 83/119 (69.75%), Query Frame = 1 
Query:    1 DVPCVVAGYGPGFETYHNENLAATDLQFTE-EFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVGVPSSTEYPFSYARDQHEHVIATLNDDCQVEKWDQYGDNQEQNDV 354
            D  C  AGY PGF+ +HNEN    D+  +E ++W  +  V+D  FD+KF  N   N+A+VRY++TVTTTDG+S+GVP++ EYPF+    QHEHV+  ++DDC    WDQYGD+QEQ DV
Sbjct:   85 DSVCAAAGYDPGFQRFHNENFTG-DIPVSEPDYWAGAFAVVDLSFDMKFTNNPSPNRASVRYIETVTTTDGLSLGVPATFEYPFNVTILQHEHVLVDVDDDCNFVLWDQYGDDQEQTDV 202
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BLAST of mRNA_Ecto-sp13_S_contig7426.18606.1 vs. uniprot
Match: A0A7S2I0Z0_9STRA (Hypothetical protein n=1 Tax=Helicotheca tamesis TaxID=374047 RepID=A0A7S2I0Z0_9STRA)

HSP 1 Score: 114 bits (286), Expect = 3.240e-25
Identity = 60/151 (39.74%), Postives = 86/151 (56.95%), Query Frame = 1 
Query:    1 DVPCVVAGYGPG-FETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVGVPSSTEYPFSYARDQHEHVIATLNDDCQVEKWDQYGDNQEQNDVFDATNAVICVIGASGLPIEECD-VTPPDEPEV 447
            DV C  AGY    F   HN     T +  +  FW  +  +LD   D     N+G NQA++RYV+ V  T+G   G+P S+++PF     QHEH + T++DDC++  WDQYGDN+EQ+DV +A   ++C +G S    E C  + PPD P +
Sbjct:   91 DVACSSAGYNSSSFVKLHNGVDTNTVIDSSGSFWAGAFALLDIIMDYNHQMNIGPNQASIRYVEKVIFTNGAEFGLPPSSDFPFGAEMIQHEHALVTVDDDCKMVLWDQYGDNKEQSDVDEAAMMILCAVGLS----EMCSSIPPPDSPPI 237
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BLAST of mRNA_Ecto-sp13_S_contig7426.18606.1 vs. uniprot
Match: D8LG98_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LG98_ECTSI)

HSP 1 Score: 108 bits (271), Expect = 8.100e-24
Identity = 57/132 (43.18%), Postives = 78/132 (59.09%), Query Frame = 1 
Query:    1 DVPCVVAGYGPGFETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVGVPSST-EYPFSYARDQHEHVIATLNDDCQVEKWDQYGDNQEQNDVFDA-TNAVICVIG 390
            D  C  A Y PGF+ +HNE            +W  +  V+D  FDVKF  N   N A+VRY++TVTT+DG S+G+P    +YPF++   QHEH I  ++D+C+   W QYGDN+EQ DV  A  N + C+ G
Sbjct:   59 DSACAAAAYDPGFQRFHNEESTGAMAVSDPGWWAGAFSVVDISFDVKFTNNPAPNTASVRYLETVTTSDGRSLGLPDPRFDYPFNFTVVQHEHAIGHVDDNCKFTFWGQYGDNKEQTDVDAAIANMMSCMGG 190
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BLAST of mRNA_Ecto-sp13_S_contig7426.18606.1 vs. uniprot
Match: K0TCL1_THAOC (Uncharacterized protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0TCL1_THAOC)

HSP 1 Score: 105 bits (263), Expect = 3.480e-22
Identity = 48/130 (36.92%), Postives = 82/130 (63.08%), Query Frame = 1 
Query:    1 DVPCVVAGYGPGFETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVGVPSSTEYPFSYARDQHEHVIATLNDDCQVEKWDQYGDNQEQNDVFDATNAVICVIG 390
            D+ CVV  Y P F   HN  +  TD   T++FW+ + +++D        +N+  NQA++RY +TVTTTDG  +G+P+S ++P++    Q+E  + T++D+C++  WDQ+GD  EQ +V  A + ++C +G
Sbjct:   41 DIACVVDSYAPDFVKMHNGKV--TDTPITKDFWSGTFMLVDIAMYYDIQENVAINQASLRYTETVTTTDGSDLGLPASNDFPWNQTFVQNEEALITVDDNCKMRLWDQHGDEAEQREVERAADEILCSVG 168
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BLAST of mRNA_Ecto-sp13_S_contig7426.18606.1 vs. uniprot
Match: A0A3N2DK43_9GAMM (Uncharacterized protein n=1 Tax=Sinobacterium caligoides TaxID=933926 RepID=A0A3N2DK43_9GAMM)

HSP 1 Score: 79.0 bits (193), Expect = 1.530e-13
Identity = 44/128 (34.38%), Postives = 62/128 (48.44%), Query Frame = 1 
Query:   10 CVVAGYGPGFETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDGVSVGVPSSTEYPFSYARDQHEHVIATLNDDCQVEKWDQYGDNQEQNDVFDATNAVICVIGA 393
            C  AGY P F   HN  L   ++    E+W  +  V DFD DV   +  G    ++RYV+TV+   G                  QHEH + T++ DC++ +WDQYGDN+EQ  V  A    +  +GA
Sbjct:   50 CAYAGYAPDFIKRHNGELVTVNMS-GPEYWRGAFAVADFDLDVNHVRRAGLKAVSLRYVETVSLVTGQRF--------------QQHEHALVTVDKDCKIIEWDQYGDNKEQLAVDQAVLTYLNAVGA 162
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BLAST of mRNA_Ecto-sp13_S_contig7426.18606.1 vs. uniprot
Match: K0RDD3_THAOC (Uncharacterized protein (Fragment) n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0RDD3_THAOC)

HSP 1 Score: 60.1 bits (144), Expect = 6.480e-6
Identity = 35/109 (32.11%), Postives = 56/109 (51.38%), Query Frame = 1 
Query:    1 DVPCVVAGYGPGFETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYKNLGSNQATVRYVQTVTTTDG------VSVGVPSSTEYPFSYARDQHEHVIATLNDDCQV 309
            D+ CVV  Y P F   HN  +  TD   T++FW+ + +++D        +N+ +NQA++RY +TVTTTDG       S+      +  F ++ D  E VI  +   C +
Sbjct:  389 DIACVVDSYAPDFVKMHNGKV--TDTPITKDFWSGTFMLVDIAMYYDVQENVATNQASLRYTETVTTTDGSVKTFKTSINRTLFAQKCFVFSSDSDEEVIEKVQIVCDL 495
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig7426.18606.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 9
Match NameE-valueIdentityDescription
D7FWX7_ECTSI9.700e-9998.68Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JJ76_9PHAE6.260e-9897.35Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5J7U4_9PHAE1.070e-3152.10Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FMJ9_ECTSI8.330e-3151.26Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A7S2I0Z0_9STRA3.240e-2539.74Hypothetical protein n=1 Tax=Helicotheca tamesis T... [more]
D8LG98_ECTSI8.100e-2443.18Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
K0TCL1_THAOC3.480e-2236.92Uncharacterized protein n=1 Tax=Thalassiosira ocea... [more]
A0A3N2DK43_9GAMM1.530e-1334.38Uncharacterized protein n=1 Tax=Sinobacterium cali... [more]
K0RDD3_THAOC6.480e-632.11Uncharacterized protein (Fragment) n=1 Tax=Thalass... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig7426contigEcto-sp13_S_contig7426:4987..7510 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score310.8
Seed ortholog evalue3.4e-82
Seed eggNOG ortholog2880.D7FWX7
Model size1547
Hectar predicted targeting categoryother localisation
Exons2
EggNOG OGs2ERFA@1,2SU8J@2759
Cds size456
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464011.5003748-UTR-Ecto-sp13_S_contig7426:4986..60771681464011.5003748-UTR-Ecto-sp13_S_contig7426:4986..6077Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig7426 4987..6077 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464011.5110574-CDS-Ecto-sp13_S_contig7426:6077..64511681464011.5110574-CDS-Ecto-sp13_S_contig7426:6077..6451Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7426 6078..6451 -
1681464011.5205834-CDS-Ecto-sp13_S_contig7426:7428..75101681464011.5205834-CDS-Ecto-sp13_S_contig7426:7428..7510Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7426 7429..7510 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig7426.18606.1prot_Ecto-sp13_S_contig7426.18606.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig7426 6078..7510 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig7426.18606.1

>prot_Ecto-sp13_S_contig7426.18606.1 ID=prot_Ecto-sp13_S_contig7426.18606.1|Name=mRNA_Ecto-sp13_S_contig7426.18606.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=152bp
DVPCVVAGYGPGFETYHNENLAATDLQFTEEFWTLSLLVLDFDFDVKFYK
NLGSNQATVRYVQTVTTTDGVSVGVPSSTEYPFSYARDQHEHVIATLNDD
CQVEKWDQYGDNQEQNDVFDATNAVICVIGASGLPIEECDVTPPDEPEVA
Q*
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mRNA from alignment at Ecto-sp13_S_contig7426:4987..7510-

Legend: polypeptideCDSUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig7426.18606.1 ID=mRNA_Ecto-sp13_S_contig7426.18606.1|Name=mRNA_Ecto-sp13_S_contig7426.18606.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2524bp|location=Sequence derived from alignment at Ecto-sp13_S_contig7426:4987..7510- (Ectocarpus species13 EcNAP12_S_4_19m)
GATGTTCCTTGCGTGGTGGCTGGCTACGGCCCAGGGTTCGAGACTTACCA
CAACGAGAACTTGGCTGCCACAGACCTTCAATGTAAGAGGTGGCGTGCGG
AGAGGCTGTCTCTTGCCATCATATGAACATCTACCTACACAGCAGCCGTG
TCTCTTGTTCTTTATTTAGAGCTTGCCTGCCGGTGCCCCTGGCAGAGGGA
TACAAGTGTGTATGTCATTCTTAGAAGCATCGACCGCACAAATCTGCTGA
GCATCGAAGAAAGCATCAGTCTCAGTGTGGTTGGAAACACAAAACATTGG
TACTTCTAAATGCCATGAACTGGGACCCCGTAAGGGGCAGTCGACGTGCG
TGTGTGTGTTCGTCCTAGCTATTCTGGTGCCGCCATACAGGCCTTTCGGC
GTGTATACCTGACGTATTGGCCGGAGTAACAAGAAGAGGGTTCACACAGG
AGCTTTTTTTGTCGTTTTCGGTTGTTGTTTCGGTGCCCGCCCCACCTTTC
TTTGCACTTGCTGCGCTTTCCTCTGGTTTTATCGCGGGAAGGGTCTAGCC
ATCATACGCCCTCGTCGACCATGAAGCCGATCTGTGGCTACTCGAGACCT
AGATCGCTTTCCACTTTTTACGGATTGTTGGATATATATGCCCCGTAGTG
AGTGAGATGATGGCGGCAGACTGCGGTTGAGGAACTCTTCGTGCCTTCAT
TGCATTTTTTAATTATATTTTCAATTTTTTGTTTGCCGCTGACAGCCGAA
TGTTCTGGTGGCACAGTAGAACCTACATGGTGCAAGTTAACCTATGCCCC
GTAGTGAGTGAGATGATGGCGGCAGACTGCGGTTGAGGAACTCTTCGTGC
CTTCATTGCATTTTTTAATTATATTTTCAATTTTTTGTTTGCCGCTGACA
GCCGAATGTTCTGGTGGCACAGTAGAACCTACATGGTGCAAGTTAACCCT
ACAATCTACGATGAATCCCTGGCCTCCATCGCTTCCTCTGAGCCTCAAGT
AGCCCTCCTTTCCTTGATGCCCCGCTCCCATCGACCCTAACCGTCCATCC
TCTTCACAGTCACCGAAGAGTTTTGGACCCTCAGTCTCTTGGTGCTGGAC
TTTGACTTCGACGTGAAGTTCTACAAGAACCTTGGCTCTAATCAAGCGAC
GGTACGCTACGTTCAGACCGTGACGACCACTGACGGGGTGAGCGTGGGAG
TGCCGTCGTCTACCGAGTACCCTTTCAGCTATGCCCGCGACCAGCACGAG
CATGTTATTGCGACGCTCAACGACGACTGCCAGGTGGAAAAATGGGACCA
GTATGGCGACAACCAGGAACAAAACGACGTGTTCGACGCAACCAACGCTG
TGATCTGCGTTATCGGCGCCTCCGGTCTCCCCATCGAAGAATGTGACGTT
ACTCCCCCGGATGAACCCGAGGTCGCCCAGTGAGGTGATGGCTCAAGACC
GGACTGAACTATCTGCTTTGACCCACGCGCTGATAAGGGCCGTGTGTGTG
ACAGTACCTGGAGGCCAGATTGGTTCGGCTGAGATGACTTTCGAGATGAC
GTTGTACAATTTTTCGTTGATGGTGGTCTCGACGACCAAGCCTGACGACG
CGGGTTCTTAGCAGTAGCCGCCGCGTACTTGATATGGCCTCGGATTGCTT
CTATGACAGTGCTAGAAGGTGTATGAGACCTGATCTGTTGCATTTCGTTT
TTCAGATGCATTTTGTTTTCAAGAGGAACAACAAAGTAGCCGTTTCGAAG
CGGGGCACAAATGAATGCCGTTAGTTCGCGATGACCTACTCGCAGTTTCG
TGTGGGTATCGTTGTTGCGATCATCAATAGACAGTCGTGCCGTCTCGAAG
CGGGACATGAATGACATTTGTGCGTGAAACGACCGATCCGCAGTCAGTCT
CTTGTGGGGATTGTTGTTCCTGAGTTTCACTTGCAGTAGGTGCCAGGTGT
AGAATATCGGTAGTCGTGGTCGAACTTAGCAATAGCACTTGGCAATGGAC
GACGACGCCGTTCGTAGAGACAGGAGTTTGTTGCATTTGAGGGTGTTGCT
GGGCGGTGTTTTCGGAGCAAGGGACACGGCATGGTTCTGTTTGTCAAGGG
TTAAGCGTTGAGCGACGATGACGCGCCGCCTTCCTCGTGCGTTGGATAGC
ACCCGATTCCATATGATAAGGCAATGCCCTGCGTTGAGCAATAAGACTGT
GTTTAATATAGTGATGCGAACGTTGCAAGTTCCTCGGTAGAGGGTCAACC
CATCTTTGTCCTCTTCTGGTGAAACATCAACCACTAGTTTTTTTAAGGTC
CCGCGATCAACTTAAAAAACGACGGTCTCTCGATCTTGGCAAACCGAACA
GTGCACGCACCCATCGCGTGTCAACCGTTGGTGGTAGGTGTGAGGTGAGG
AGCTCGTCCGGGTAAAACTCTACTTCGTGTTCGACATGAACAACGGCTTC
CCGCGCAAATTGCGAGGAAGAGATCACCCTGAGTGATCTCAATGTAGGAG
GGACACAAATATGCATTGAGCCCA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig7426:4987..7510-

>mRNA_Ecto-sp13_S_contig7426.18606.1 ID=mRNA_Ecto-sp13_S_contig7426.18606.1|Name=mRNA_Ecto-sp13_S_contig7426.18606.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=456bp|location=Sequence derived from alignment at Ecto-sp13_S_contig7426:4987..7510- (Ectocarpus species13 EcNAP12_S_4_19m)
GATGTTCCTTGCGTGGTGGCTGGCTACGGCCCAGGGTTCGAGACTTACCA
CAACGAGAACTTGGCTGCCACAGACCTTCAATTCACCGAAGAGTTTTGGA
CCCTCAGTCTCTTGGTGCTGGACTTTGACTTCGACGTGAAGTTCTACAAG
AACCTTGGCTCTAATCAAGCGACGGTACGCTACGTTCAGACCGTGACGAC
CACTGACGGGGTGAGCGTGGGAGTGCCGTCGTCTACCGAGTACCCTTTCA
GCTATGCCCGCGACCAGCACGAGCATGTTATTGCGACGCTCAACGACGAC
TGCCAGGTGGAAAAATGGGACCAGTATGGCGACAACCAGGAACAAAACGA
CGTGTTCGACGCAACCAACGCTGTGATCTGCGTTATCGGCGCCTCCGGTC
TCCCCATCGAAGAATGTGACGTTACTCCCCCGGATGAACCCGAGGTCGCC
CAGTGA
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