mRNA_Ecto-sp13_S_contig7122.18222.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig7122.18222.1
Unique NamemRNA_Ecto-sp13_S_contig7122.18222.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig7122.18222.1 vs. uniprot
Match: D7G5R3_ECTSI (EF-hand domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5R3_ECTSI)

HSP 1 Score: 479 bits (1232), Expect = 1.300e-163
Identity = 249/263 (94.68%), Postives = 253/263 (96.20%), Query Frame = 1 
Query:    1 ETAKMFDHLDANGDGSIDLAEFTRFCLEIPSMTWKAERARRGILGNNAGEHGDRELEKAARDMANETVSASDAVEGGVGGELAKLEPLVHLGNIFYRGEKFFWRTKDLIEVVIQLNEAKGFLAIPASAAAEAEILNDLKAKRLAAKTEHAMGGSGSGSGSRGGEIDANKEQAGDQQQEDQALSEKAHMAYIADYIVHRITMPDGDGNLLQPDVTRPEEEVNPGLPAGGEGSSTGSGAKKTKRGSAAARPGLMRTTFDEWDTLL 789
            ETAKMFD LDANGDGSIDLAEFTRFCLEIPSMTWKAERARRGILGNNAGE GDRELEKAARDMANETVSA+DA EGG+GGELAKLEPLVHLG IFYRGEKFFWRTKDLIEVV+QLNEAKGFLAIPASAAAEAEILNDLKAKRLAAKTEHAMGGSGS    RGGE DANKEQAGDQQQEDQALSEKAHMAYIADYIVHRITMPDG+GNLLQPDVTRPEEEVNPGLPAGGEGSSTGSGA KTKRGSAAARPGLMRTTFDEWDTLL
Sbjct:  317 ETAKMFDQLDANGDGSIDLAEFTRFCLEIPSMTWKAERARRGILGNNAGEDGDRELEKAARDMANETVSANDAAEGGLGGELAKLEPLVHLGKIFYRGEKFFWRTKDLIEVVVQLNEAKGFLAIPASAAAEAEILNDLKAKRLAAKTEHAMGGSGS----RGGESDANKEQAGDQQQEDQALSEKAHMAYIADYIVHRITMPDGNGNLLQPDVTRPEEEVNPGLPAGGEGSSTGSGATKTKRGSAAARPGLMRTTFDEWDTLL 575
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BLAST of mRNA_Ecto-sp13_S_contig7122.18222.1 vs. uniprot
Match: D8LCP2_ECTSI (EF-hand domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LCP2_ECTSI)

HSP 1 Score: 88.2 bits (217), Expect = 3.860e-16
Identity = 78/267 (29.21%), Postives = 116/267 (43.45%), Query Frame = 1 
Query:    4 TAKMF----DHLDANGDGSIDLAEFTRFCLEIPSMTWKAERARRGILGNNAGEHGDRE-----LEKAARDMANETVSASDAVEGGVGGELAKLEPLVHLGNIFYRGEKFFWRTKDLIEVVIQLNEAKGFLAIPASAAAEAEILNDLKA--------------KRLAAKTEHAMGGSGSGSGSRGGEIDANKEQAGDQQQEDQALSEKAHMAYIADYIVHRITMPDG---DGNLLQPDVTRPEEEVNPGLPAGGEGSSTGSGAKKTKR 726
            T+++F    +  D +G G+I+L+EF R+CL+I +M WKAER RR   G  +G HG R      +++AA  MA E   A          +L   +P   LG + Y G K FWRT + + + +  N+ K  +AI      E+E    LK               +R+A  TE   G           EI    E A  +      L +KA  + ++DY++ R+ + DG   DGN      +         L  GG G  T   +  T R
Sbjct:  126 TSQLFGVFLETFDTDGSGTIELSEFIRWCLDIDTMAWKAERVRRTTGGQTSGGHGKRGDGEIGIDEAAARMAGEVAQA----------QLPPEQPRFPLGTLIYEGSKLFWRTGESLRIAMHENKEKHCIAISTMLEGESEPFPVLKVDSRLIRVNGKDAVLERIAEMTEADKG-----------EIQRLPEDARAK------LFDKARASVLSDYLLARLKLIDGRDADGNATDTQASAAAISAAARLGLGGVGPGTAPESASTAR 365
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BLAST of mRNA_Ecto-sp13_S_contig7122.18222.1 vs. uniprot
Match: D8LSJ9_ECTSI (EF-hand domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSJ9_ECTSI)

HSP 1 Score: 71.6 bits (174), Expect = 1.060e-10
Identity = 41/115 (35.65%), Postives = 62/115 (53.91%), Query Frame = 1 
Query:   28 DANGDGSIDLAEFTRFCLEIPSMTWKAERARRGILGNNAGEHGDRELEKAARDMANETVSASDAVEGGVGGELAKLEPLVHLGNIFYRGEKFFWRTKDLIEVVIQLNEAKGFLAI 372
            D +G+G+++L EF  FCL IPS+ W+AE+ARR  L N   +    EL  AAR +A    S                   V +G + ++G +FFWR+K+ I+V + LN  +G + I
Sbjct:  175 DTDGNGTVELDEFMTFCLSIPSLPWRAEKARR--LNNAESKD---ELNVAARKIARRRSSLR----------------AVPIGELVHQGSRFFWRSKETIDVALHLNADEGLMTI 268
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BLAST of mRNA_Ecto-sp13_S_contig7122.18222.1 vs. uniprot
Match: A0A835YNE5_9STRA (EF-hand domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YNE5_9STRA)

HSP 1 Score: 64.3 bits (155), Expect = 3.810e-8
Identity = 42/124 (33.87%), Postives = 62/124 (50.00%), Query Frame = 1 
Query:    1 ETAKMFDHLDANGDGSIDLAEFTRFCLEIPSMTWKAERARRGILGNNAGEHGDRELEKAARDMANETVSASDAVEGGVGGELAKLEPLVHLGNIFYRGEKFFWRTKDLIEVVIQLNEAKGFLAI 372
            E  ++  H DAN +G I+L+EF  FC+ IPS+ WKAERARR      +   G   L +AAR+      S   AV+               LG   +  + FFWR++  ++VV+  N  +G + I
Sbjct:   86 ELDELMAHFDANRNGRIELSEFMEFCMAIPSLAWKAERARR-----MSAVDGPEALTEAARNSERRR-STCKAVD---------------LGEKIHDRKMFFWRSQQNLDVVLHENIGEGVVII 188
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BLAST of mRNA_Ecto-sp13_S_contig7122.18222.1 vs. uniprot
Match: A0A7S2BJX7_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2BJX7_9STRA)

HSP 1 Score: 57.0 bits (136), Expect = 1.100e-5
Identity = 36/124 (29.03%), Postives = 55/124 (44.35%), Query Frame = 1 
Query:    1 ETAKMFDHLDANGDGSIDLAEFTRFCLEIPSMTWKAERARRGILGNNAGEHGDRELEKAARDMANETVSASDAVEGGVGGELAKLEPLVHLGN-----------IFYRGEKFFWRTKDLIEVVI 339
            E +++F  LD +G G+ID+ EF   C  IP + WKAE+ R             + +E AA+    E  +      G  G  +  LE   H  +           + Y G K FWRT + I++V+
Sbjct:   78 ELSEIFQDLDDDGGGTIDVDEFKAHCYNIPRLAWKAEKIR-------FEREKTKSIEDAAKMAEREATNLPLTPSGSTGAIMHALEDHRHQKDSAAAANMQSKEVIYEGTKLFWRTHEKIDIVM 194
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig7122.18222.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
D7G5R3_ECTSI1.300e-16394.68EF-hand domain-containing protein n=1 Tax=Ectocarp... [more]
D8LCP2_ECTSI3.860e-1629.21EF-hand domain-containing protein n=1 Tax=Ectocarp... [more]
D8LSJ9_ECTSI1.060e-1035.65EF-hand domain-containing protein n=1 Tax=Ectocarp... [more]
A0A835YNE5_9STRA3.810e-833.87EF-hand domain-containing protein n=1 Tax=Tribonem... [more]
A0A7S2BJX7_9STRA1.100e-529.03Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig7122contigEcto-sp13_S_contig7122:600..4293 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score91.3
Seed ortholog evalue7.3e-16
Seed eggNOG ortholog2880.D8LCP2
Model size789
Hectar predicted targeting categoryother localisation
Exons7
EggNOG OGs2E61A@1,2SCSX@2759
Cds size777
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig7122.18222.1prot_Ecto-sp13_S_contig7122.18222.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig7122 600..4281 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463977.4677024-CDS-Ecto-sp13_S_contig7122:599..7311681463977.4677024-CDS-Ecto-sp13_S_contig7122:599..731Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7122 600..731 -
1681463977.4779842-CDS-Ecto-sp13_S_contig7122:1048..11981681463977.4779842-CDS-Ecto-sp13_S_contig7122:1048..1198Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7122 1049..1198 -
1681463977.4870088-CDS-Ecto-sp13_S_contig7122:1649..17871681463977.4870088-CDS-Ecto-sp13_S_contig7122:1649..1787Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7122 1650..1787 -
1681463977.4963202-CDS-Ecto-sp13_S_contig7122:2543..26011681463977.4963202-CDS-Ecto-sp13_S_contig7122:2543..2601Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7122 2544..2601 -
1681463977.5080595-CDS-Ecto-sp13_S_contig7122:2829..29791681463977.5080595-CDS-Ecto-sp13_S_contig7122:2829..2979Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7122 2830..2979 -
1681463977.5185409-CDS-Ecto-sp13_S_contig7122:3522..35991681463977.5185409-CDS-Ecto-sp13_S_contig7122:3522..3599Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7122 3523..3599 -
1681463977.5346856-CDS-Ecto-sp13_S_contig7122:4209..42811681463977.5346856-CDS-Ecto-sp13_S_contig7122:4209..4281Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7122 4210..4281 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463977.5464208-UTR-Ecto-sp13_S_contig7122:4281..42931681463977.5464208-UTR-Ecto-sp13_S_contig7122:4281..4293Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig7122 4282..4293 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig7122.18222.1

>prot_Ecto-sp13_S_contig7122.18222.1 ID=prot_Ecto-sp13_S_contig7122.18222.1|Name=mRNA_Ecto-sp13_S_contig7122.18222.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=259bp
MFDHLDANGDGSIDLAEFTRFCLEIPSMTWKAERARRGILGNNAGEHGDR
ELEKAARDMANETVSASDAVEGGVGGELAKLEPLVHLGNIFYRGEKFFWR
TKDLIEVVIQLNEAKGFLAIPASAAAEAEILNDLKAKRLAAKTEHAMGGS
GSGSGSRGGEIDANKEQAGDQQQEDQALSEKAHMAYIADYIVHRITMPDG
DGNLLQPDVTRPEEEVNPGLPAGGEGSSTGSGAKKTKRGSAAARPGLMRT
TFDEWDTLL
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mRNA from alignment at Ecto-sp13_S_contig7122:600..4293-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig7122.18222.1 ID=mRNA_Ecto-sp13_S_contig7122.18222.1|Name=mRNA_Ecto-sp13_S_contig7122.18222.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=3694bp|location=Sequence derived from alignment at Ecto-sp13_S_contig7122:600..4293- (Ectocarpus species13 EcNAP12_S_4_19m)
GAGACCGCCAAGATGTTCGATCATCTCGACGCCAACGGCGACGGTTCCAT
CGACCTCGCAGAGTTCACGAGGTTCTGCCTAGAGGTGTGCAAGGGAAAGA
GCTCGCCGGGCGCGACAAGTATCAACTTAGGCAAATATCGTCCCTCCCCC
GCGGGGATAAGGTCATTTCTGAACAGTGATTCCATCTTGCTCTTTTGACT
CTAACCGTCACACTATCTATTGGGTGTCGACTTCTCCGCAACTTCTTGAT
GGCAACGTTTTGGTGCCCTTGAAGGTACAAGCAACATACCGACGTCTTCC
TGATGCAATTTCATCGCACAATCTTCTCGTTTTCCCTGTCCCCGGTGGTC
TAGTTGATATCCTCGAAACCTTTAAGAAGTCAGGTCACGGGGTTGTATTC
CGGCGTAAGCGACCTCTTCTTGCAAAGGGATTTTTTGTCTCGCTTCCGCT
CCTGTCCTAGTGGATAGCGCTAGCTCGTGTGTACACAGAGGGAAGAGAGA
GCTGAACTCGTCTCGCATCAAAAAACCAAGGCAAGGAACCGTAGGAAGGG
AGGTAGAGAGGGGCCCTTCTGTGTGACCACGGGTTGGAATAAACGGCTCA
AGTACTGCTGTTAGCGCTGACTTGGAGTGAAGACCAAAAAAAGATTCTTC
GCTCTTATTCCATACCACGTATCACATGGGACAAAATGGTTCAGATTCCG
TCCATGACCTGGAAGGCCGAGCGTGCTAGGAGGGGGATCCTCGGAAACAA
CGCCGGGGAACACGGAGATCGGTAAGGACTAAGGAGTACAACAGGCACAT
CCATATCTTCATAGTTCACGCTACGGGTGCTTACCGGGCATGAACTGAGC
GATCTCCCACCACGTACAACACTATATTGGGCGGCGACGGTATTCCACAA
ACGCAAATACGAACTATGTTAGTGTCAGCCGCGTGCGCTAGGCTTAGTCT
TCCTTCTCGAAATAGTCGGCTCGGTCATCGACTTGGACTTCGCCCTCGCT
GCGGTCGAACTTTCTTCTCCTGCTCCTTCCTTGGGTTCCCGCTTTGTCAA
GAGGGAAACCAGAAGATGATTGGACGAAATTGTGCGCCAGTCGAGCATAC
CGTTATCTCACAAAAATGAATAAATCGTGAATTATCAGAATTGCTCGTGG
TTCTCACTACAAGAAAGAAATATGAAAACCACGCACAACACCCGATACCC
TCCACACAAACACGCACACACGCTGTTTCTTCCCCACGGCAACATCTCAA
AGAATCCGAACGCCGAAACAATCCCTCGTTTCCTCTCGTTCTGTTTCAAT
TGTCGGCGAGACAGCGAGTTGGAAAAGGCAGCACGGGACATGGCTAACGA
GACAGTGAGTGCCAGCGACGCCGTGGAAGGCGGTGTGGGAGGGGAGCTGG
CGAAGCTGGAGCCCTTGGTGCACCTCGGGAATATATTCTATAGGGGGGAG
AAGTTTTTCTGGAGGTAGGTGCGAGTCGGTGGGTCAATGTGTGTGACTTG
TGTGCTGCGCGCTCCACTCTTGGGCATGACTTTACTGTAATAATTAGAAT
CATGAGACTGTATTCTGAGTTATGTGGGAGATAGGCACAGGCAATTGTTC
TGTGAGTGAGGCGCCGTCGTTTCACACTAGCGGCATGTCCTCACACTTGT
TACACCTCTGGATGATGACCCTTTGGTTGGCGACGCCTGCAGGACCAAGG
ACCTGATCGAGGTCGTGATCCAGCTCAACGAGGCCAAGGGGTTCCTGGCC
GTGAGCACGTTCCACACAGAAAAACTCAAGACCTACCCCATAATCTTCAT
TGATCGTGCTAAGGTACAGTACACGGCCCTTTGGTCGAAAACGCCGCACT
CTTCGGCTACAGCAAGAGTGGCCCATAGAGAAGCATCGACCCCAAGAATC
ATATTTGTGAACGTACATCAAGTAAGGTGTGTGATGCACAAGCCGCTTTC
ACACAGGAGGAAAACAAACACTGCAGAGTGCTTTTGTTCGCTGCGCTGCC
AATGACAAACTTTGAGTTAGCTCAGCAATAAAGCCTGCACTCAACGATGG
CGATGAAGCATCCTGTTGAGCATCGATCGACCCCGAAAGGAACGGCCCTT
AGTCTAGTTAGTAGCAGGCCCCGAAGCAAAGTACTTCTCGCATCAGCCGT
GTACATGCGCCTGCAGAGCGTTCACGGCCGGGCGCTCACCATCTAGTGCC
GTAAGCCCAACGAAAAGGTTGTCATTGGGGTTAAGAGCGTCGCTACAGCC
CTGATGCGGTGAGGAGTATCTCAACTTGTGAGCCCCGGTCCTGAGGCGCT
CGACACACACCTTAGCGAGGAGTTGGTCATAAAGAAGATACCCGACAAAC
CTTGAACGGGGGCTATGGGTAGCGCGACCACTATCAGAACTAAACCACCC
GTGAGATATGGAACGAAATGATTATTGTCATACATAAACACGAACTAAGC
TCGAGAGGGTCTGCGCATTTCCCCGTTCCGTCACTGCTGCTATTTCCTCC
GTGTAGATCCCCGCAAGCGCTGCGGCCGAGGCCGAAATCTTGAACGACCT
CAAGGCCAAGCGATTGGCGGCCAAGACCGAGCACGCCATGGGAGGATCAG
GATCAGGATCAGGATCAAGAGGAGGCGAAATCGACGCGAACAAGGTGTTG
CAGGACATGATGCCAACAACATTGATTTTTTTTTATTTCACCTCGTACTA
GAATGGAGCACTATAAGGGTAGGGTCACAGGTTTGGGAATCCGAAACACA
TGTCCGTGTGTGGATATTTTATCTTGAAATCAGCCGCCATCAACCTGCAG
TCTCGTCGCAATGCGCTAGGTGGTATTCGTCCGTTGGCTCCTTGCCCGTC
TCGTGCACTTGTTTACTCAGGACCACGCATGTTGGCTGTTTCATGTTGTG
TTCTACACGCTGGGGTGAAAGCGCCCGTCCCGTTGGGTGCAGAACTTATT
TCCGTGTGATTGTGCAGTGTCCAGGACCATGAACAAAAACCATCTTGAGG
TAGCGAGCCCCATCCAATCCCATTGTTCGAGTGTAGTAGCGGCCTTTGTG
ACACGTTCATTCACCAAGCACGCTTCCGAACTTGAATTAACTCAGGAGCA
GGCGGGGGACCAGCAGCAAGAGGACCAGGCGCTGTCGGAGAAGGCGCATA
TGGCTTACATAGCCGACTACATCGTACACCGCATCACGATGCCGGACGGT
GACGGGAACCTTCTACAGCCTGACGTCACTCGCCCCGAGGAAGAGGTGGG
CGCGCCAAGAGCGATGTCTTCCCCACGGTGAAGATAGATGGATGAAAAGC
GTCAACATATCGATACCTCCGTCGGTCTTAAAGCCTTATATGCGTGCTTG
AAGGACGATAATAGTATCGTTTGTCTGGAATTTTTCCAAGGTTTTACTCT
CTCTTTCGTAAGGTCGTCGGGCACTCGGCCTGAGCCTCCTTTGCGGTCAG
TTGGTCTAAATGGCATCTGGACTCACGTTGGCTCTACCCCAGACCAACGG
TGTAGCTGTTTCTCTAAGTGTGTCACGAGCTACGCATTGATGGCCCTCCC
TCGTCCTCTAAGGTAAACCCCGGTTTGCCGGCCGGCGGGGAAGGCAGCAG
CACCGGATCGGGCGCCAAGAAGACGAAACGAGGGAGTGCTGCGGCGAGGC
CGGGGCTAATGAGAACCACGTTCGACGAGTGGGACACCCTCTTG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig7122:600..4293-

>mRNA_Ecto-sp13_S_contig7122.18222.1 ID=mRNA_Ecto-sp13_S_contig7122.18222.1|Name=mRNA_Ecto-sp13_S_contig7122.18222.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=777bp|location=Sequence derived from alignment at Ecto-sp13_S_contig7122:600..4293- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGTTCGATCATCTCGACGCCAACGGCGACGGTTCCATCGACCTCGCAGA
GTTCACGAGGTTCTGCCTAGAGATTCCGTCCATGACCTGGAAGGCCGAGC
GTGCTAGGAGGGGGATCCTCGGAAACAACGCCGGGGAACACGGAGATCGC
GAGTTGGAAAAGGCAGCACGGGACATGGCTAACGAGACAGTGAGTGCCAG
CGACGCCGTGGAAGGCGGTGTGGGAGGGGAGCTGGCGAAGCTGGAGCCCT
TGGTGCACCTCGGGAATATATTCTATAGGGGGGAGAAGTTTTTCTGGAGG
ACCAAGGACCTGATCGAGGTCGTGATCCAGCTCAACGAGGCCAAGGGGTT
CCTGGCCATCCCCGCAAGCGCTGCGGCCGAGGCCGAAATCTTGAACGACC
TCAAGGCCAAGCGATTGGCGGCCAAGACCGAGCACGCCATGGGAGGATCA
GGATCAGGATCAGGATCAAGAGGAGGCGAAATCGACGCGAACAAGGAGCA
GGCGGGGGACCAGCAGCAAGAGGACCAGGCGCTGTCGGAGAAGGCGCATA
TGGCTTACATAGCCGACTACATCGTACACCGCATCACGATGCCGGACGGT
GACGGGAACCTTCTACAGCCTGACGTCACTCGCCCCGAGGAAGAGGTAAA
CCCCGGTTTGCCGGCCGGCGGGGAAGGCAGCAGCACCGGATCGGGCGCCA
AGAAGACGAAACGAGGGAGTGCTGCGGCGAGGCCGGGGCTAATGAGAACC
ACGTTCGACGAGTGGGACACCCTCTTG
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