mRNA_Ecto-sp13_S_contig7075.18156.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig7075.18156.1
Unique NamemRNA_Ecto-sp13_S_contig7075.18156.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig7075.18156.1 vs. uniprot
Match: D8LKS5_ECTSI (LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LKS5_ECTSI)

HSP 1 Score: 263 bits (673), Expect = 2.160e-71
Identity = 161/318 (50.63%), Postives = 202/318 (63.52%), Query Frame = 2 
Query:   14 ARLWDTHKAYLKSGYLDKAYLRFLWIHRKIQGAQSSAVILSAAEFESIVSTMARLLVMYGSHDPDKLVVPARLPEYGPESVMSPDNIAEIAVTMQCSFGQVYPPPGIVGRFLAWSTKQVYRYEECWQHGAFLWYNYDRGKYRVLLFES-IDEEIREGKAVRIAGLMFCVQAPPAMAPTVLTALRASLQHLVADEAYGYPGLKCLMSFEDPAEVKSTLLRDLRALLDNRDDLVKAVDRXXXXXXXXXXXXXXX----SGVANQLVQQELLAASAERKEYPYPRLVILVPDGEAYGN--NERIQRVGWDRWRKAWESLSL 946
            A L+D H  Y +SG L++AYLRFLW+HRKI+G     V L+  EFESIVSTM RLLVMY + D D LVVPARL EYG + +  PD +  + + ++CSFGQVYPPPGIVGRFLAWS +QV  Y ECWQHGAF  Y Y   +Y V L+ES ++E  ++G     AGL   +Q PP  A  VL  LR SL+ LV+D AYGYPGL   M F    E++S+LL  L ++LD           XXXXXXXXXX          G+  QL++Q+LL AS E+  YP  RLV++ P+ E   +  +ERIQR GWDRW KA   +  
Sbjct:  663 APLFDIHNVYCRSGRLNRAYLRFLWMHRKIEGP-GPTVDLTDPEFESIVSTMERLLVMYTARDADNLVVPARLQEYGDQRI--PDRLTNMVLKIECSFGQVYPPPGIVGRFLAWSAQQVADYGECWQHGAFFSYKYQYARYDVFLYESEVEEAHQDGTVDMFAGLTLGIQGPPPQAAEVLEELRKSLESLVSDSAYGYPGLVGPMYFRPAEEIESSLLVRLGSVLDXXXXXXXXXXXXXXXXXXXXXSLEETVRKLGGIVTQLLEQDLLPASDEQSAYP--RLVLVRPELETAEDMTHERIQRAGWDRWTKALSEMGF 975
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BLAST of mRNA_Ecto-sp13_S_contig7075.18156.1 vs. uniprot
Match: D8LK68_ECTSI (LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LK68_ECTSI)

HSP 1 Score: 125 bits (314), Expect = 1.140e-25
Identity = 97/321 (30.22%), Postives = 152/321 (47.35%), Query Frame = 2 
Query:   11 FARLWDTHKAYLKSGYLDKAYLRFLWIHRK--IQGAQSSAVILSAAEFESIVSTMARLLVMYGSHDP-----DKLVVPARLPEYGPESVMSPD-NIAEIAVTMQCSFGQVYPPPGIVGRFLAWSTKQVYRYEECWQHGAFLWYNYDRGKYRVLLFES-IDEEIREGKAVRIAGLMFCVQAPPAMAPTVLTALRASLQHLVADEAYGYPGLKCLMSFEDPAEVKSTLLRDLRALLDNRDDLVKAVDRXXXXXXXXXXXXXXXSGVANQLVQQELLAASAERKEYPYPRLVILVP---------DGEAYGNNERIQRVGWDRW 919
            +  L +TH  +++ G L++ YLRFLW+HRK  ++G  + A  L    F+S+V TM + L +Y SH       D  +VPARLPE+G ++++     + E+ V     F + + P GI+GRFLA+++ +     +CWQHGA + +  D  ++ VLL E+ I+++I  G++     +  CV+   A A  VL  +   L  L+ D  +GYPGL+ L  FE+    +S             D      +R                 V    + +    +  +  E PYPRLV+L P           E   N     R  WDRW
Sbjct: 1650 YKNLCETHACFIRQGRLNRDYLRFLWVHRKLEVEGDYAGAQ-LDKDAFDSMVDTMIKYLFIYPSHGECSNAEDCHIVPARLPEHGNDNILEESIGLGEVVVGKTAWFLRSHAPTGIIGRFLAFTSSRFEASGKCWQHGAHIQWKKDSNEHDVLLCETTIEKDIIPGQST-FPAIAICVKGKTAEAKGVLREVGEQLLSLLQDNIHGYPGLR-LPIFEELNITQS-------------DQFQVHFERYLATQLAKILEDTRRESVR---MFRAAFPSYGDPVELPYPRLVLLKPVETTSIQETQAEESVNGAIQLRRTWDRW 1951
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BLAST of mRNA_Ecto-sp13_S_contig7075.18156.1 vs. uniprot
Match: A0A6H5JR01_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JR01_9PHAE)

HSP 1 Score: 106 bits (264), Expect = 4.780e-20
Identity = 90/321 (28.04%), Postives = 145/321 (45.17%), Query Frame = 2 
Query:   11 FARLWDTHKAYLKSGYLDKAYLRFLWIHRKIQGAQSSA-VILSAAEFESIVSTMARLLVMYGSHDPDK-----LVVPARLPEYGPESVMSPD-NIAEIAVTMQCSFGQVYPPPGIVGRFLAWSTKQVYRYEECWQHGAFLWYNYDRGKYRVLLFES-IDEEIREGKAVRIAGLMFCVQAPPAMAPTVLTALRASLQHLVADEAYGYPGLKCLMSFEDPAEVK-STLLRDLRALLDNRDDLVKAVDRXXXXXXXXXXXXXXXSGVANQLVQQELLAASAERKEYPYPRLVILVP---------DGEAYGNNERIQRVGWDRW 919
            +  L +TH  +++ G L++ YLRFLW+HRK++     A V L    F+S+V TM + L +Y SH          +VPARLPE G ++V+     + E+ V     F + + P GI+G FLA+++ +     ECWQHGA + +  D  ++ +LL E+ I+++I  G    +  +  CV+   A A  VL  +   L  L+ D  +        + FE     K +T+L D R                                  +  + +    +  E  E  YPRLV+L P           E+    ++++R  WDRW
Sbjct:  204 YKNLCETHTCFIRQGRLNRKYLRFLWVHRKLEVEGGYAGVQLENDTFDSMVDTMIKYLFIYPSHGECSKAEGFYIVPARLPEIGKDNVLEESIGLDEVVVGKTAWFLRSHAPTGIIGCFLAFTSSRFEASGECWQHGAHIQWKKDSNEHDMLLCETTIEKDIIPGPGT-LPAIAICVKGKTAEAKGVLREVGEQLLSLLKDVIH--------VHFERYLATKLATILEDTRR--------------------------------ESVRMFRAAFPSYGEHVELLYPRLVLLKPVETTSIQETQVESVSGAKKLRRT-WDRW 482
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BLAST of mRNA_Ecto-sp13_S_contig7075.18156.1 vs. uniprot
Match: A0A6H5JPJ1_9PHAE (Roc domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JPJ1_9PHAE)

HSP 1 Score: 63.5 bits (153), Expect = 1.460e-6
Identity = 64/223 (28.70%), Postives = 97/223 (43.50%), Query Frame = 3 
Query:  948 LTWVPCGPDGLGYPIE--QLKDWVKRCVPLMKASLWILRVAVGTVSNVD-LPLNEVLEAAVKATG---AAVLEGVGTEVPNSVDNDPVP-------VFG----EGATVEQER------GFRDLQ-------------GSAYKFLCDFMREAEPSTPRR-----------LWGCVPCRRPVPPPPDLTPSV--DWKDSMVQVRNRDG----RWAWVLKEHKEAY 1457
            LT   CG DGLGY +E    ++W+K C+P++K SLW+LRV +  V+  D LP++ V+E   +  G   A +L  +  E   S   D V        VFG    E A   Q+R      G  D Q             G   ++LC + R +  +TP +           + G VP   PV P   + P+    ++  M     + G     WAWV + +K+ +
Sbjct:  964 LTAGTCGKDGLGYKVEIQGWQEWLKACLPVIKVSLWLLRVGISVVTRADVLPVDGVIETLEEVAGEEAAGILGEIDLEGRRSSGQDLVDKVNAQEQVFGTAYAELAKWVQQREDKIGDGGDDAQTAGNTVGGGAADGGRGCRWLCSWWRRSHNNTPAQPPDAGCAMSGNVTGGVPAA-PVAPVSTVAPAGKRSFESDMELASRQRGTEAPEWAWVRRVNKDKW 1185
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig7075.18156.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 4
Match NameE-valueIdentityDescription
D8LKS5_ECTSI2.160e-7150.63LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus s... [more]
D8LK68_ECTSI1.140e-2530.22LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus s... [more]
A0A6H5JR01_9PHAE4.780e-2028.04Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JPJ1_9PHAE1.460e-628.70Roc domain-containing protein n=1 Tax=Ectocarpus s... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig7075contigEcto-sp13_S_contig7075:3270..6163 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score89.4
Seed ortholog evalue3.6e-15
Seed eggNOG ortholog2880.D8LK68
Model size2253
KEGG rclassRC00043,RC00070,RC00090,RC00992,RC02896
KEGG koko:K03800,ko:K16666
KEGG ReactionR07770,R07771,R11143
KEGG Pathwayko00785,ko01100,map00785,map01100
Hectar predicted targeting categoryother localisation
Exons4
EggNOG free text desc.regulation of response to stimulus
EggNOG OGsCOG4886@1,KOG0619@2759
EC6.3.1.20
Cds size1020
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig7075.18156.1prot_Ecto-sp13_S_contig7075.18156.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig7075 3819..5096 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463970.6539614-UTR-Ecto-sp13_S_contig7075:3269..33051681463970.6539614-UTR-Ecto-sp13_S_contig7075:3269..3305Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig7075 3270..3305 +
1681463970.6720297-UTR-Ecto-sp13_S_contig7075:3688..38181681463970.6720297-UTR-Ecto-sp13_S_contig7075:3688..3818Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig7075 3689..3818 +
1681463970.7249265-UTR-Ecto-sp13_S_contig7075:5096..61631681463970.7249265-UTR-Ecto-sp13_S_contig7075:5096..6163Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig7075 5097..6163 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463970.6862783-CDS-Ecto-sp13_S_contig7075:3818..46091681463970.6862783-CDS-Ecto-sp13_S_contig7075:3818..4609Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7075 3819..4609 +
1681463970.7033634-CDS-Ecto-sp13_S_contig7075:4662..47421681463970.7033634-CDS-Ecto-sp13_S_contig7075:4662..4742Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7075 4663..4742 +
1681463970.7144377-CDS-Ecto-sp13_S_contig7075:4947..50961681463970.7144377-CDS-Ecto-sp13_S_contig7075:4947..5096Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig7075 4948..5096 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig7075.18156.1

>prot_Ecto-sp13_S_contig7075.18156.1 ID=prot_Ecto-sp13_S_contig7075.18156.1|Name=mRNA_Ecto-sp13_S_contig7075.18156.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=340bp
MARLLVMYGSHDPDKLVVPARLPEYGPESVMSPDNIAEIAVTMQCSFGQV
YPPPGIVGRFLAWSTKQVYRYEECWQHGAFLWYNYDRGKYRVLLFESIDE
EIREGKAVRIAGLMFCVQAPPAMAPTVLTALRASLQHLVADEAYGYPGLK
CLMSFEDPAEVKSTLLRDLRALLDNRDDLVKAVDRLGKTVDRLEVTASKL
SGVANQLVQQELLAASAERKEYPYPRLVILVPDGEAYGNNERIQRVGWDR
WRKAWESLSLPDVGTMWPRRAWIPYRAAEGLGQAVRSFDEGVAVDLAGGS
WHRVKCGPAPERSAGSCCEGNRCRSAGGGGHRGPQLCRQ*
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mRNA from alignment at Ecto-sp13_S_contig7075:3270..6163+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig7075.18156.1 ID=mRNA_Ecto-sp13_S_contig7075.18156.1|Name=mRNA_Ecto-sp13_S_contig7075.18156.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2894bp|location=Sequence derived from alignment at Ecto-sp13_S_contig7075:3270..6163+ (Ectocarpus species13 EcNAP12_S_4_19m)
TGATGATGATTTTGCGCGGCTTTGGGACACGCACAAGTGAGTTAAGGATA
AAAAACTATCTCGAACTGAACCTAGTTCTAGAGGCTACTCGAGGGAAGGC
TGCCATAAACGTACGGCATATGTCGAGGAACAATCAAGCACCTGTTATGT
GTCCTGATATTTTCTCAGCTTGTTCAACAGTTGCGTGTACCTGTTCTCCC
CAGTGAGGATCGCCTCGCAGCTACCTTGAGGATGGCAGTTGCGTACAACG
TGTCTCTCTTACCCTTGAACAAGTGTTCATCAAGTGCACGGCTGCGGGGA
TTGTAATGTCGGGTTGGACAAATCCACCGCGACGGCTGGGGCAGTGGTGA
ATCTATCTAGAGAATCGCTTTTTTAACCATCGGCTCACACCCATGTCCTT
GTATGTGCATCGTTGGCAGAGCGTACCTCAAGTCTGGTTACCTGGACAAA
GCTTACCTTCGCTTTCTCTGGATCCATCGCAAGATCCAGGGTGCCCAGAG
TTCAGCGGTGATCCTCTCCGCTGCCGAGTTCGAATCCATTGTTTCGACAA
TGGCAAGGCTGCTGGTAATGTACGGATCTCACGACCCGGACAAACTTGTG
GTACCGGCCCGTCTCCCAGAATACGGTCCCGAGAGCGTGATGTCTCCGGA
CAACATTGCGGAGATTGCAGTCACGATGCAGTGTTCGTTCGGTCAAGTCT
ATCCCCCACCTGGAATTGTCGGACGTTTCCTTGCCTGGTCCACGAAGCAA
GTCTACCGCTACGAGGAATGCTGGCAGCATGGGGCTTTCCTCTGGTACAA
CTATGATCGAGGAAAGTACAGGGTGTTGTTATTTGAGTCGATAGATGAGG
AGATTCGGGAAGGGAAGGCAGTGAGGATTGCTGGTCTCATGTTCTGTGTG
CAGGCGCCACCAGCCATGGCTCCAACGGTTCTCACAGCGTTGAGAGCGTC
GCTGCAACACCTAGTGGCGGACGAAGCGTATGGATACCCAGGACTGAAGT
GCTTGATGTCGTTCGAGGACCCGGCTGAAGTAAAGTCAACCTTGCTTAGG
GACCTCCGTGCCCTCCTGGACAATCGAGATGATCTGGTGAAAGCCGTAGA
TAGGCTAGGTAAGACCGTAGATAGGCTGGAGGTGACAGCGTCGAAGTTGA
GTGGAGTCGCGAACCAGTTGGTCCAGCAGGAGTTGTTGGCCGCGAGTGCC
GAGCGAAAGGAGTATCCGTACCCGCGCCTGGTGATCTTGGTACCGGACGG
CGAAGCATATGGGAATAATGAACGGATCCAGCGTGTGGGGTGGGACAGGT
GGAGGAAGGCCTGGGAAAGCTTGAGTCTGCCTGACGTGGGGTTGCACCAC
AAGTTTCGCCTCCGTTTCCTTTGTGAGCACAGCCTCGAAGAAGTACCATG
TGGCCCCGACGGGCTTGGATACCCTATCGAGCAGCTGAAGGATTGGGTCA
AGCGGTGCGTTCCTTTGATGAAGGTGAGTTTTGGCGTGCACCTGGTAGGT
CTTCTTGTTCGCTTCGTTTGTTCCGTTTCTCTCCGGCGCTCCTTGAGCTC
ATACCGCGCATCTGTCGGCTATTCGTTTACGTTCGTGGTAATGCCTGGCA
TCCCAGGGGCTTGTGCTGGATCAATTGCCTCCCATATCGGCGCCTCAATG
TTCGTCGTGCTCGCTGCGCTTTCCTCAGGCGTCGCTGTGGATCTTGCGGG
TGGCAGTTGGCACCGTGTCAAATGTGGACCTGCCCCTGAACGAAGTGCTG
GAAGCTGCTGTGAAGGCAACCGGTGCCGCAGTGCTGGAGGGGGTGGGCAC
AGAGGTCCCCAACTCTGTCGACAATGATCCGGTCCCGGTGTTTGGCGAGG
GGGCGACGGTGGAACAGGAACGGGGGTTCAGGGATCTGCAGGGCTCGGCT
TACAAATTCCTGTGCGACTTTATGCGGGAAGCCGAGCCGTCAACTCCTCG
GCGTTTGTGGGGTTGTGTTCCTTGCCGTCGCCCAGTCCCCCCACCGCCCG
ATCTCACGCCAAGTGTTGACTGGAAGGATAGTATGGTTCAAGTGAGGAAT
CGCGACGGACGGTGGGCGTGGGTCTTGAAAGAGCACAAGGAGGCATATTG
TGGCGGAAACGGAACAGCTGCGTCAAGACGTTCGCCTCGACCTCCGATCT
CATGATGAAATGAGATTGGATCAGCAAATCGCTTTAGTTTATTAGTGATT
TATTTGCGGTCAGAATCCGGCTTGCAGGGGAAGAGAGCGGTGCCGGTAGG
GGGAAGCGGAGCAATTTACGCGACGAATTGGTGCAGTGTAGATGTCATTT
TGCGAGATGACATAGCTCGATTTGCGTGAAAGGTGAAAGAGGGTGTCCGC
GGTCTTGTAGAATGACCTTTCCAAATGCTCGGTGTTGCAGTTGCAGGAAG
CAATCTCTGTGTCGTCTCTTTGTACCGACTTTTGGCTGTCACAGATTCGA
TTCTGCTTGAGGAGATATGTGAGGATCGAAGAGGGCGTTGGTGGGCGTGC
TTCGGTGGTTTTTTTCGGGCGTGGTGGTGTCCGGTTTTTGTTGTGTCTCA
GTCGGCACGTAAGCTAGCTCGGTGAGTGGGTGGCCTGCTTTCTATTTTCC
GGGTTTGCAGGATGGAATGGGGATAGACCGTGGCATGTAGAGTAGTTGGG
GCCTTACCTGTGGTTTGGCGTTGGCAGTAACGAGAAATTTCGGATTTTAT
TGAAACGTTTATGGTTTATGTCCGTTGGAGCTCGCTGTAAACGCCTCACA
CAAGCTCCCCGGCCCACAACAACTGGAGGAGTGAAGCATGGAGTGAACGC
CGAAGTCACCAGATGATGCTTGCTGTGCCAACCCGGAAGAATCTATTTTG
CAGACTCCGTGTACGTTTTTGATCGACGGAGTCATCCTTCGGAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig7075:3270..6163+

>mRNA_Ecto-sp13_S_contig7075.18156.1 ID=mRNA_Ecto-sp13_S_contig7075.18156.1|Name=mRNA_Ecto-sp13_S_contig7075.18156.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=1020bp|location=Sequence derived from alignment at Ecto-sp13_S_contig7075:3270..6163+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGCAAGGCTGCTGGTAATGTACGGATCTCACGACCCGGACAAACTTGT
GGTACCGGCCCGTCTCCCAGAATACGGTCCCGAGAGCGTGATGTCTCCGG
ACAACATTGCGGAGATTGCAGTCACGATGCAGTGTTCGTTCGGTCAAGTC
TATCCCCCACCTGGAATTGTCGGACGTTTCCTTGCCTGGTCCACGAAGCA
AGTCTACCGCTACGAGGAATGCTGGCAGCATGGGGCTTTCCTCTGGTACA
ACTATGATCGAGGAAAGTACAGGGTGTTGTTATTTGAGTCGATAGATGAG
GAGATTCGGGAAGGGAAGGCAGTGAGGATTGCTGGTCTCATGTTCTGTGT
GCAGGCGCCACCAGCCATGGCTCCAACGGTTCTCACAGCGTTGAGAGCGT
CGCTGCAACACCTAGTGGCGGACGAAGCGTATGGATACCCAGGACTGAAG
TGCTTGATGTCGTTCGAGGACCCGGCTGAAGTAAAGTCAACCTTGCTTAG
GGACCTCCGTGCCCTCCTGGACAATCGAGATGATCTGGTGAAAGCCGTAG
ATAGGCTAGGTAAGACCGTAGATAGGCTGGAGGTGACAGCGTCGAAGTTG
AGTGGAGTCGCGAACCAGTTGGTCCAGCAGGAGTTGTTGGCCGCGAGTGC
CGAGCGAAAGGAGTATCCGTACCCGCGCCTGGTGATCTTGGTACCGGACG
GCGAAGCATATGGGAATAATGAACGGATCCAGCGTGTGGGGTGGGACAGG
TGGAGGAAGGCCTGGGAAAGCTTGAGTCTGCCTGACGTGGGTACCATGTG
GCCCCGACGGGCTTGGATACCCTATCGAGCAGCTGAAGGATTGGGTCAAG
CGGTGCGTTCCTTTGATGAAGGCGTCGCTGTGGATCTTGCGGGTGGCAGT
TGGCACCGTGTCAAATGTGGACCTGCCCCTGAACGAAGTGCTGGAAGCTG
CTGTGAAGGCAACCGGTGCCGCAGTGCTGGAGGGGGTGGGCACAGAGGTC
CCCAACTCTGTCGACAATGA
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