mRNA_Ecto-sp13_S_contig68930.17864.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig68930.17864.1
Unique NamemRNA_Ecto-sp13_S_contig68930.17864.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig68930.17864.1 vs. uniprot
Match: D8LJD4_ECTSI (Glutamate--cysteine ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LJD4_ECTSI)

HSP 1 Score: 148 bits (373), Expect = 1.070e-39
Identity = 76/91 (83.52%), Postives = 78/91 (85.71%), Query Frame = 1 
Query:    7 KVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVANSAVPP------------WHVRGPVANSAYIPDVVINPHPRFGALTKNIRVR 243
            +VERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVA+SAVPP            WHVRGPVANSAYIPDVVINPHPRFGALTKNIR R
Sbjct:   92 RVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVASSAVPPSASDSSERPSSRWHVRGPVANSAYIPDVVINPHPRFGALTKNIRAR 182
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BLAST of mRNA_Ecto-sp13_S_contig68930.17864.1 vs. uniprot
Match: A0A836CE91_9STRA (Glutamate--cysteine ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CE91_9STRA)

HSP 1 Score: 111 bits (278), Expect = 7.880e-27
Identity = 58/79 (73.42%), Postives = 63/79 (79.75%), Query Frame = 1 
Query:    7 KVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVANSAVPPWHVRGPVANSAYIPDVVINPHPRFGALTKNIRVR 243
            +VERNMRLRRKRILSVL P E+APT+TC P MGVGDFVA    P   V GP+ANSA IPD +INPHPRFGALT NIR R
Sbjct:   87 RVERNMRLRRKRILSVLAPDEIAPTVTCLPTMGVGDFVA----PAGPVPGPIANSAAIPDSIINPHPRFGALTANIRAR 161
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BLAST of mRNA_Ecto-sp13_S_contig68930.17864.1 vs. uniprot
Match: A0A1V9Z600_9STRA (Glutamate--cysteine ligase n=3 Tax=Saprolegniaceae TaxID=4764 RepID=A0A1V9Z600_9STRA)

HSP 1 Score: 102 bits (255), Expect = 1.030e-23
Identity = 51/81 (62.96%), Postives = 64/81 (79.01%), Query Frame = 1 
Query:    1 VHKVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVANSAVPPWHVRGPVANSAYIPDVVINPHPRFGALTKNIRVR 243
            + +VE++MRLRR R+LSVL+P E+APT+T FPL+GVGD+VA    PP +  GP+ANS YI D +INPHPRFG LT NIR R
Sbjct:  121 LRRVEQSMRLRRARLLSVLKPNEIAPTVTAFPLLGVGDYVA----PPVNRVGPIANSLYIDDRIINPHPRFGTLTANIRTR 197
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BLAST of mRNA_Ecto-sp13_S_contig68930.17864.1 vs. uniprot
Match: A0A1V9ZIF2_9STRA (Glutamate--cysteine ligase n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZIF2_9STRA)

HSP 1 Score: 97.8 bits (242), Expect = 5.950e-22
Identity = 48/81 (59.26%), Postives = 61/81 (75.31%), Query Frame = 1 
Query:    1 VHKVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVANSAVPPWHVRGPVANSAYIPDVVINPHPRFGALTKNIRVR 243
            + +VE++MRLRR R+LS L+  E+APT+T FPL+GVGD++     PP   RGP+ANS YI D +INPHPRFG LT NIR R
Sbjct:  121 LRRVEQSMRLRRARLLSALKKNEIAPTVTAFPLLGVGDYIT----PPVVKRGPIANSLYISDSIINPHPRFGTLTANIRKR 197
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BLAST of mRNA_Ecto-sp13_S_contig68930.17864.1 vs. uniprot
Match: A0A6G0WEE2_9STRA (Glutamate--cysteine ligase n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WEE2_9STRA)

HSP 1 Score: 97.1 bits (240), Expect = 1.090e-21
Identity = 49/81 (60.49%), Postives = 60/81 (74.07%), Query Frame = 1 
Query:    1 VHKVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVANSAVPPWHVRGPVANSAYIPDVVINPHPRFGALTKNIRVR 243
            + +VE +MRLRR R+LSVL+  E+ PT+T FPL+GVGDF     VPP    GP+A SAYI D++INPHPRFG LT NIR R
Sbjct:  134 LRRVEPSMRLRRARLLSVLKDNEICPTVTAFPLLGVGDFT----VPPSLPNGPIAKSAYISDMIINPHPRFGTLTANIRKR 210
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BLAST of mRNA_Ecto-sp13_S_contig68930.17864.1 vs. uniprot
Match: D7FP20_ECTSI (Glutamate--cysteine ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FP20_ECTSI)

HSP 1 Score: 97.1 bits (240), Expect = 1.100e-21
Identity = 50/79 (63.29%), Postives = 57/79 (72.15%), Query Frame = 1 
Query:    7 KVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVANSAVPPWHVRGPVANSAYIPDVVINPHPRFGALTKNIRVR 243
            +VERNMRLRRKR+L+ L   E++PTMT FPL GVGDF      PP    GP A S ++PD VINPHPRF ALTKNIR R
Sbjct:  146 RVERNMRLRRKRLLATLADDEISPTMTNFPLFGVGDFTN----PPAEAGGPTAQSKFVPDSVINPHPRFAALTKNIRER 220
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BLAST of mRNA_Ecto-sp13_S_contig68930.17864.1 vs. uniprot
Match: A0A7S3HC51_9STRA (Glutamate--cysteine ligase (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3HC51_9STRA)

HSP 1 Score: 92.0 bits (227), Expect = 2.100e-21
Identity = 46/79 (58.23%), Postives = 58/79 (73.42%), Query Frame = 1 
Query:    7 KVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVANSAVPPWHVRGPVANSAYIPDVVINPHPRFGALTKNIRVR 243
            +VERNM LRR+R+LS+L+P E+AP +TCFPL+GV DF+    V P     P + S +IPD +INPHPRF ALT NIR R
Sbjct:  120 RVERNMILRRRRLLSLLKPNEIAPYVTCFPLLGVADFI----VDPQPFAAPHSKSDFIPDYIINPHPRFAALTTNIRSR 194
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BLAST of mRNA_Ecto-sp13_S_contig68930.17864.1 vs. uniprot
Match: G5AA29_PHYSP (Glutamate--cysteine ligase n=1 Tax=Phytophthora sojae (strain P6497) TaxID=1094619 RepID=G5AA29_PHYSP)

HSP 1 Score: 95.9 bits (237), Expect = 2.810e-21
Identity = 50/81 (61.73%), Postives = 59/81 (72.84%), Query Frame = 1 
Query:    1 VHKVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVANSAVPPWHVRGPVANSAYIPDVVINPHPRFGALTKNIRVR 243
            + KVE NMRLRR R+L++L   E+AP++T FP+MGVGDF     VPP    GPVA S YI DVVINPHPRF  LT+NIR R
Sbjct:  136 LRKVEGNMRLRRARLLALLHEDEIAPSVTAFPMMGVGDFT----VPPSQPHGPVAMSDYISDVVINPHPRFATLTRNIRQR 212
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BLAST of mRNA_Ecto-sp13_S_contig68930.17864.1 vs. uniprot
Match: A0A485KIR0_9STRA (Glutamate--cysteine ligase n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KIR0_9STRA)

HSP 1 Score: 95.5 bits (236), Expect = 3.810e-21
Identity = 48/81 (59.26%), Postives = 57/81 (70.37%), Query Frame = 1 
Query:    1 VHKVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVANSAVPPWHVRGPVANSAYIPDVVINPHPRFGALTKNIRVR 243
            + +VE NMRLRR R+LSVL+  E+ PT+T FPL+GVGDF      P    RGP+A S YI D +INPHPRFG LT NIR R
Sbjct:  121 LRRVEPNMRLRRARLLSVLKENEICPTVTAFPLLGVGDFTVPHTTP----RGPIAKSEYISDGIINPHPRFGTLTANIRKR 197
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BLAST of mRNA_Ecto-sp13_S_contig68930.17864.1 vs. uniprot
Match: A0A484EDH9_BRELC (Glutamate--cysteine ligase n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484EDH9_BRELC)

HSP 1 Score: 95.1 bits (235), Expect = 5.200e-21
Identity = 48/81 (59.26%), Postives = 59/81 (72.84%), Query Frame = 1 
Query:    1 VHKVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVANSAVPPWHVRGPVANSAYIPDVVINPHPRFGALTKNIRVR 243
            + KVE NMRLRR R+L++L   E+AP++T FP+MGVGDF   S+ P    RGPVA S Y+ D +INPHPRF  LTKNIR R
Sbjct:  132 LRKVEDNMRLRRARLLALLHEDEIAPSVTAFPMMGVGDFTVPSSQP----RGPVAMSDYVSDAIINPHPRFATLTKNIRQR 208
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig68930.17864.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LJD4_ECTSI1.070e-3983.52Glutamate--cysteine ligase n=1 Tax=Ectocarpus sili... [more]
A0A836CE91_9STRA7.880e-2773.42Glutamate--cysteine ligase n=1 Tax=Tribonema minus... [more]
A0A1V9Z600_9STRA1.030e-2362.96Glutamate--cysteine ligase n=3 Tax=Saprolegniaceae... [more]
A0A1V9ZIF2_9STRA5.950e-2259.26Glutamate--cysteine ligase n=1 Tax=Thraustotheca c... [more]
A0A6G0WEE2_9STRA1.090e-2160.49Glutamate--cysteine ligase n=1 Tax=Aphanomyces eut... [more]
D7FP20_ECTSI1.100e-2163.29Glutamate--cysteine ligase n=2 Tax=Ectocarpus TaxI... [more]
A0A7S3HC51_9STRA2.100e-2158.23Glutamate--cysteine ligase (Fragment) n=1 Tax=Spum... [more]
G5AA29_PHYSP2.810e-2161.73Glutamate--cysteine ligase n=1 Tax=Phytophthora so... [more]
A0A485KIR0_9STRA3.810e-2159.26Glutamate--cysteine ligase n=1 Tax=Aphanomyces ste... [more]
A0A484EDH9_BRELC5.200e-2159.26Glutamate--cysteine ligase n=1 Tax=Bremia lactucae... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig68930contigEcto-sp13_S_contig68930:355..633 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score143.3
Seed ortholog evalue4.6e-32
Seed eggNOG ortholog2880.D8LJD4
Preferred nameGCLC
Model size243
KEGG rclassRC00064,RC00090
KEGG koko:K11204
KEGG ReactionR00894,R10993
KEGG Pathwayko00270,ko00480,ko01100,ko04212,ko04216,map00270,map00480,map01100,map04212,map04216
KEGG ModuleM00118
Hectar predicted targeting categoryother localisation
GOsGO:0000096,GO:0000166,GO:0000287,GO:0000302,GO:0000303,GO:0000305,GO:0000902,GO:0000904,GO:0001101,GO:0001678,GO:0002009,GO:0002165,GO:0003008,GO:0003013,GO:0003018,GO:0003254,GO:0003674,GO:0003824,GO:0004357,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005829,GO:0005975,GO:0005996,GO:0006082,GO:0006355,GO:0006518,GO:0006520,GO:0006534,GO:0006536,GO:0006575,GO:0006732,GO:0006749,GO:0006750,GO:0006766,GO:0006767,GO:0006790,GO:0006807,GO:0006810,GO:0006811,GO:0006820,GO:0006857,GO:0006915,GO:0006950,GO:0006979,GO:0006996,GO:0007005,GO:0007275,GO:0007346,GO:0007399,GO:0007409,GO:0007444,GO:0007472,GO:0007476,GO:0007552,GO:0007560,GO:0007568,GO:0007584,GO:0008015,GO:0008150,GO:0008152,GO:0008219,GO:0008637,GO:0009058,GO:0009064,GO:0009069,GO:0009266,GO:0009408,GO:0009410,GO:0009605,GO:0009612,GO:0009628,GO:0009636,GO:0009653,GO:0009719,GO:0009725,GO:0009743,GO:0009746,GO:0009749,GO:0009791,GO:0009886,GO:0009887,GO:0009888,GO:0009889,GO:0009890,GO:0009892,GO:0009893,GO:0009894,GO:0009896,GO:0009966,GO:0009968,GO:0009987,GO:0009991,GO:0010033,GO:0010035,GO:0010038,GO:0010243,GO:0010468,GO:0010556,GO:0010558,GO:0010604,GO:0010605,GO:0010628,GO:0010629,GO:0010646,GO:0010648,GO:0010941,GO:0012501,GO:0014070,GO:0014823,GO:0015711,GO:0015833,GO:0016043,GO:0016595,GO:0016597,GO:0016874,GO:0016879,GO:0016881,GO:0017076,GO:0017109,GO:0019184,GO:0019219,GO:0019222,GO:0019725,GO:0019748,GO:0019752,GO:0019852,GO:0022008,GO:0023051,GO:0023057,GO:0030030,GO:0030154,GO:0030162,GO:0030182,GO:0030435,GO:0030554,GO:0030587,GO:0031175,GO:0031323,GO:0031324,GO:0031325,GO:0031326,GO:0031327,GO:0031329,GO:0031331,GO:0031396,GO:0031397,GO:0031399,GO:0031400,GO:0031406,GO:0031667,GO:0032268,GO:0032269,GO:0032270,GO:0032354,GO:0032434,GO:0032436,GO:0032501,GO:0032502,GO:0032553,GO:0032555,GO:0032559,GO:0032868,GO:0032869,GO:0032870,GO:0032989,GO:0032990,GO:0032991,GO:0033500,GO:0034097,GO:0034284,GO:0034635,GO:0034641,GO:0034698,GO:0035107,GO:0035114,GO:0035120,GO:0035150,GO:0035220,GO:0035239,GO:0035295,GO:0035690,GO:0035728,GO:0035729,GO:0036094,GO:0042176,GO:0042221,GO:0042391,GO:0042398,GO:0042493,GO:0042542,GO:0042592,GO:0042593,GO:0042886,GO:0042939,GO:0042981,GO:0043043,GO:0043066,GO:0043067,GO:0043069,GO:0043167,GO:0043168,GO:0043169,GO:0043177,GO:0043200,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043434,GO:0043436,GO:0043523,GO:0043524,GO:0043531,GO:0043603,GO:0043604,GO:0043648,GO:0043934,GO:0044237,GO:0044238,GO:0044249,GO:0044271,GO:0044272,GO:0044281,GO:0044344,GO:0044424,GO:0044444,GO:0044464,GO:0044550,GO:0044752,GO:0045454,GO:0045732,GO:0045862,GO:0045892,GO:0045934,GO:0046677,GO:0046685,GO:0046686,GO:0046688,GO:0046872,GO:0046937,GO:0046938,GO:0046982,GO:0046983,GO:0048037,GO:0048468,GO:0048471,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048563,GO:0048569,GO:0048583,GO:0048585,GO:0048646,GO:0048666,GO:0048667,GO:0048699,GO:0048707,GO:0048729,GO:0048731,GO:0048736,GO:0048737,GO:0048812,GO:0048856,GO:0048858,GO:0048869,GO:0048878,GO:0050662,GO:0050789,GO:0050794,GO:0050801,GO:0050865,GO:0050866,GO:0050880,GO:0050896,GO:0051171,GO:0051172,GO:0051173,GO:0051179,GO:0051181,GO:0051186,GO:0051188,GO:0051234,GO:0051246,GO:0051247,GO:0051248,GO:0051252,GO:0051253,GO:0051409,GO:0051703,GO:0051704,GO:0051716,GO:0051726,GO:0051881,GO:0051900,GO:0055082,GO:0060255,GO:0060429,GO:0060548,GO:0060562,GO:0061136,GO:0061564,GO:0061687,GO:0065007,GO:0065008,GO:0070555,GO:0070848,GO:0070887,GO:0071214,GO:0071229,GO:0071230,GO:0071241,GO:0071248,GO:0071260,GO:0071276,GO:0071310,GO:0071322,GO:0071326,GO:0071331,GO:0071333,GO:0071363,GO:0071371,GO:0071372,GO:0071375,GO:0071407,GO:0071417,GO:0071495,GO:0071496,GO:0071585,GO:0071702,GO:0071704,GO:0071705,GO:0071774,GO:0071840,GO:0072337,GO:0072348,GO:0080090,GO:0090066,GO:0090702,GO:0097066,GO:0097067,GO:0097068,GO:0097069,GO:0097159,GO:0097237,GO:0097367,GO:0097501,GO:0098754,GO:0098849,GO:0099120,GO:0104004,GO:0120036,GO:0120039,GO:1901214,GO:1901215,GO:1901265,GO:1901363,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901652,GO:1901653,GO:1901698,GO:1901699,GO:1901700,GO:1901701,GO:1901800,GO:1902494,GO:1902679,GO:1903050,GO:1903052,GO:1903320,GO:1903321,GO:1903362,GO:1903364,GO:1903506,GO:1903507,GO:1904386,GO:1904387,GO:1990170,GO:1990748,GO:2000058,GO:2000060,GO:2000112,GO:2000113,GO:2000489,GO:2000490,GO:2001141,GO:2001233,GO:2001234,GO:2001236,GO:2001237
Exons2
EggNOG free text desc.glutamate-cysteine ligase activity
EggNOG OGsKOG3754@1,KOG3754@2759
EC6.3.2.2
Cds size222
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463946.6610749-UTR-Ecto-sp13_S_contig68930:354..3751681463946.6610749-UTR-Ecto-sp13_S_contig68930:354..375Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig68930 355..375 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463946.6735983-CDS-Ecto-sp13_S_contig68930:375..4941681463946.6735983-CDS-Ecto-sp13_S_contig68930:375..494Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig68930 376..494 +
1681463946.691933-CDS-Ecto-sp13_S_contig68930:530..6331681463946.691933-CDS-Ecto-sp13_S_contig68930:530..633Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig68930 531..633 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig68930.17864.1prot_Ecto-sp13_S_contig68930.17864.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig68930 376..633 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig68930.17864.1

>prot_Ecto-sp13_S_contig68930.17864.1 ID=prot_Ecto-sp13_S_contig68930.17864.1|Name=mRNA_Ecto-sp13_S_contig68930.17864.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=74bp
MRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVANSAVPPWHVRGPVANS
AYIPDVVINPHPRFGALTKNIRVR
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mRNA from alignment at Ecto-sp13_S_contig68930:355..633+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig68930.17864.1 ID=mRNA_Ecto-sp13_S_contig68930.17864.1|Name=mRNA_Ecto-sp13_S_contig68930.17864.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=279bp|location=Sequence derived from alignment at Ecto-sp13_S_contig68930:355..633+ (Ectocarpus species13 EcNAP12_S_4_19m)
GTCCATAAGGTGGAACGCAATATGCGCCTCCGCCGCAAGCGTATTCTGAG
CGTCCTCCGGCCTGGCGAGGTAGCCCCGACGATGACCTGCTTCCCACTCA
TGGGGGTGGGCGACTTTGTAGCCAATTCCGCCGTCCCCCCGTCCGCCTCC
GACTCGAGCGAGAGACTGTCCTCGAGATGGCACGTGCGGGGGCCCGTGGC
GAACTCCGCGTACATCCCTGACGTCGTGATTAACCCGCACCCGCGGTTCG
GAGCCCTGACGAAGAACATTCGGGTGAGG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig68930:355..633+

>mRNA_Ecto-sp13_S_contig68930.17864.1 ID=mRNA_Ecto-sp13_S_contig68930.17864.1|Name=mRNA_Ecto-sp13_S_contig68930.17864.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=222bp|location=Sequence derived from alignment at Ecto-sp13_S_contig68930:355..633+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGCGCCTCCGCCGCAAGCGTATTCTGAGCGTCCTCCGGCCTGGCGAGGT
AGCCCCGACGATGACCTGCTTCCCACTCATGGGGGTGGGCGACTTTGTAG
CCAATTCCGCCGTCCCCCCATGGCACGTGCGGGGGCCCGTGGCGAACTCC
GCGTACATCCCTGACGTCGTGATTAACCCGCACCCGCGGTTCGGAGCCCT
GACGAAGAACATTCGGGTGAGG
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