mRNA_Ecto-sp13_S_contig6256.16983.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig6256.16983.1
Unique NamemRNA_Ecto-sp13_S_contig6256.16983.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig6256.16983.1 vs. uniprot
Match: A0A6H5K1Z4_9PHAE (Pyridoxal 5'-phosphate synthase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K1Z4_9PHAE)

HSP 1 Score: 196 bits (497), Expect = 8.150e-58
Identity = 94/107 (87.85%), Postives = 100/107 (93.46%), Query Frame = 1 
Query:    1 MNGVLALQVTEKESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQVCACSITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVMDDKWIMQRLQP 321
            + GV+  +VT++ESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQ    SITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVV+DDKWIMQRLQP
Sbjct:  460 IEGVMT-KVTDQESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQDKFSSITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVVDDKWIMQRLQP 565
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BLAST of mRNA_Ecto-sp13_S_contig6256.16983.1 vs. uniprot
Match: A0A7K1C4T1_9ACTN (Pyridoxine/pyridoxamine 5'-phosphate oxidase n=2 Tax=root TaxID=1 RepID=A0A7K1C4T1_9ACTN)

HSP 1 Score: 129 bits (323), Expect = 2.650e-35
Identity = 58/102 (56.86%), Postives = 72/102 (70.59%), Query Frame = 1 
Query:   16 ALQVTEKESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQVCACSITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVMDDKWIMQRLQP 321
            A +VT  ES+EYF +RPRGSQV AWA  QSRP+ESR ++E    D +       +VPRPPH+GG+R+ P RIEFWKGR  R+HDRIVY    D W ++RLQP
Sbjct:  110 AEKVTPDESSEYFATRPRGSQVAAWASEQSRPIESRAAIEDKFDDAEERFAGEADVPRPPHWGGYRIVPSRIEFWKGRSNRMHDRIVYTRDGDSWAVERLQP 211
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BLAST of mRNA_Ecto-sp13_S_contig6256.16983.1 vs. uniprot
Match: A0A327X2C5_9BACT (Pyridoxine/pyridoxamine 5'-phosphate oxidase n=1 Tax=Larkinella arboricola TaxID=643671 RepID=A0A327X2C5_9BACT)

HSP 1 Score: 119 bits (297), Expect = 2.320e-31
Identity = 53/100 (53.00%), Postives = 73/100 (73.00%), Query Frame = 1 
Query:   22 QVTEKESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQVCACSITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVMDDKWIMQRLQP 321
            +VT  ES  YF+SRPRGSQ+GAW  HQS  VESR +LE   ++++        VPRPPH+GG+RL PDRIEFW+GR +R+HDR++Y   ++ W+++RL P
Sbjct:  115 KVTADESDAYFQSRPRGSQIGAWVSHQSSVVESRAALEQRQQELEQ-QFENQPVPRPPHWGGYRLIPDRIEFWQGRPSRLHDRVLYRKENEAWVIERLSP 213
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BLAST of mRNA_Ecto-sp13_S_contig6256.16983.1 vs. uniprot
Match: A0A7G6YLM3_9BACT (Pyridoxine/pyridoxamine 5'-phosphate oxidase n=5 Tax=Hymenobacter TaxID=89966 RepID=A0A7G6YLM3_9BACT)

HSP 1 Score: 119 bits (297), Expect = 2.500e-31
Identity = 54/97 (55.67%), Postives = 68/97 (70.10%), Query Frame = 1 
Query:   31 EKESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQVCACSITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVMDDKWIMQRLQP 321
            E  STEYF+SRPRGSQVGAWA  QS+P+  R +LEA   +V+        +PRPPH+GG+ LRP R+EFW+GR +R+HDRIVY    D W + RL P
Sbjct:  120 ETMSTEYFQSRPRGSQVGAWASPQSQPIADRETLEAREHEVESQFAGQDPLPRPPHWGGYLLRPTRVEFWQGRPSRLHDRIVYEQAGDTWRLSRLAP 216
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BLAST of mRNA_Ecto-sp13_S_contig6256.16983.1 vs. uniprot
Match: A0A6J4P779_9ACTN (Pyridoxine/pyridoxamine 5'-phosphate oxidase n=1 Tax=uncultured Rubrobacteraceae bacterium TaxID=349277 RepID=A0A6J4P779_9ACTN)

HSP 1 Score: 117 bits (294), Expect = 6.230e-31
Identity = 53/102 (51.96%), Postives = 75/102 (73.53%), Query Frame = 1 
Query:   16 ALQVTEKESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQVCACSITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVMDDKWIMQRLQP 321
            A ++++ ES  YF SRPRGS++GAW   QSRPVE RG LE  V+ ++    S+ E+PRPP +GG+R+ P+ IEFW+GR++R+HDR+VY   D  W ++RLQP
Sbjct:  111 ASRISDDESDAYFASRPRGSRLGAWVSEQSRPVEDRGVLEERVRKLEAEYESL-EIPRPPFWGGYRVEPEAIEFWQGRESRLHDRLVYRREDGVWRIERLQP 211
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BLAST of mRNA_Ecto-sp13_S_contig6256.16983.1 vs. uniprot
Match: A0A3S3ZT08_9NOCA (Pyridoxine/pyridoxamine 5'-phosphate oxidase n=1 Tax=Rhodococcus agglutinans TaxID=1644129 RepID=A0A3S3ZT08_9NOCA)

HSP 1 Score: 117 bits (293), Expect = 1.000e-30
Identity = 51/100 (51.00%), Postives = 72/100 (72.00%), Query Frame = 1 
Query:   22 QVTEKESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQVCACSITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVMDDKWIMQRLQP 321
            + +E  +T+Y+ SRPRGS++GAWA HQSRP+ SR  LEA + D         +VP PPH+GGFR+RP+ +EFW+GR  R+H+RI   ++D  WI++RLQP
Sbjct:  117 RTSEDTTTDYWNSRPRGSRLGAWASHQSRPIASRADLEAQLVDAGQHFAVDGDVPVPPHWGGFRIRPESVEFWQGRPNRMHNRIRTRLVDGAWIVERLQP 216
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BLAST of mRNA_Ecto-sp13_S_contig6256.16983.1 vs. uniprot
Match: A0A558C210_9BACT (Pyridoxine/pyridoxamine 5'-phosphate oxidase n=3 Tax=Cytophagales TaxID=768507 RepID=A0A558C210_9BACT)

HSP 1 Score: 117 bits (293), Expect = 1.030e-30
Identity = 53/97 (54.64%), Postives = 68/97 (70.10%), Query Frame = 1 
Query:   31 EKESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQVCACSITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVMDDKWIMQRLQP 321
            E  ST YF+SRPRGSQVGAWA  QS+P+ESR +LE    D++        +PRPPH+GG+ LRP RIEFW+GR +R+HDR+VY    + W + RL P
Sbjct:  121 EDVSTAYFQSRPRGSQVGAWASPQSQPIESREALEGREHDIEARFADQNPLPRPPHWGGYVLRPTRIEFWQGRPSRLHDRLVYEREGEGWKISRLAP 217
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BLAST of mRNA_Ecto-sp13_S_contig6256.16983.1 vs. uniprot
Match: UPI001998436F (Pyridoxamine 5'-phosphate oxidase n=1 Tax=Sphingobacteriaceae bacterium TaxID=2021370 RepID=UPI001998436F)

HSP 1 Score: 117 bits (292), Expect = 1.310e-30
Identity = 56/102 (54.90%), Postives = 72/102 (70.59%), Query Frame = 1 
Query:   25 VTEKESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQVCACSITE--VPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVMDD-KWIMQRLQP 321
            V+ +ES  YF SRPRGSQ+GAW+  QS+PV SR  LEA  +DV   A   T   VPRPPH+GG+RLRPD +EFW+GR +R+HDR+ Y +  D  W ++RL P
Sbjct:  115 VSPQESDAYFASRPRGSQLGAWSSDQSQPVPSREDLEAQERDV---AARFTNQPVPRPPHWGGYRLRPDYLEFWQGRPSRLHDRVAYALQSDGTWTLERLSP 213
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BLAST of mRNA_Ecto-sp13_S_contig6256.16983.1 vs. uniprot
Match: UPI001CF91AEF (pyridoxamine 5'-phosphate oxidase n=1 Tax=Penaeicola halotolerans TaxID=2793196 RepID=UPI001CF91AEF)

HSP 1 Score: 117 bits (292), Expect = 1.310e-30
Identity = 54/100 (54.00%), Postives = 71/100 (71.00%), Query Frame = 1 
Query:   22 QVTEKESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQVCACSITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVMDDKWIMQRLQP 321
            +V+   S EYF SRP+GSQ+GAWA  QS+ + SR  LE+N+KD++       +VPRPPH+GGF L PDRIEFW+GR +R+HDR+VY    D W + RL P
Sbjct:  115 KVSSLLSDEYFNSRPKGSQLGAWASPQSQKINSRSVLESNLKDLEK-EYQDRQVPRPPHWGGFELTPDRIEFWQGRASRLHDRVVYEKKADTWHINRLAP 213
[Close]
BLAST of mRNA_Ecto-sp13_S_contig6256.16983.1 vs. uniprot
Match: A0A850BTV9_9DELT (Pyridoxine/pyridoxamine 5'-phosphate oxidase n=1 Tax=Polyangiaceae bacterium TaxID=2268199 RepID=A0A850BTV9_9DELT)

HSP 1 Score: 116 bits (291), Expect = 1.810e-30
Identity = 54/101 (53.47%), Postives = 71/101 (70.30%), Query Frame = 1 
Query:   22 QVTEKESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKDVQVCACSITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVMDDK-WIMQRLQP 321
            +++E+ES  YFK+RPRGSQ+GAWA  QSRP+ESR  LE  V+++        E+PRPPH+GGFRL P  IE W+GR  R+HDR++Y   DD  W  +RL P
Sbjct:  113 KISEEESDAYFKARPRGSQIGAWASEQSRPIESRRELERRVEEL-AATYRDREIPRPPHWGGFRLSPVAIELWQGRLDRLHDRVLYSRADDGGWTRERLSP 212
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig6256.16983.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5K1Z4_9PHAE8.150e-5887.85Pyridoxal 5'-phosphate synthase n=1 Tax=Ectocarpus... [more]
A0A7K1C4T1_9ACTN2.650e-3556.86Pyridoxine/pyridoxamine 5'-phosphate oxidase n=2 T... [more]
A0A327X2C5_9BACT2.320e-3153.00Pyridoxine/pyridoxamine 5'-phosphate oxidase n=1 T... [more]
A0A7G6YLM3_9BACT2.500e-3155.67Pyridoxine/pyridoxamine 5'-phosphate oxidase n=5 T... [more]
A0A6J4P779_9ACTN6.230e-3151.96Pyridoxine/pyridoxamine 5'-phosphate oxidase n=1 T... [more]
A0A3S3ZT08_9NOCA1.000e-3051.00Pyridoxine/pyridoxamine 5'-phosphate oxidase n=1 T... [more]
A0A558C210_9BACT1.030e-3054.64Pyridoxine/pyridoxamine 5'-phosphate oxidase n=3 T... [more]
UPI001998436F1.310e-3054.90Pyridoxamine 5'-phosphate oxidase n=1 Tax=Sphingob... [more]
UPI001CF91AEF1.310e-3054.00pyridoxamine 5'-phosphate oxidase n=1 Tax=Penaeico... [more]
A0A850BTV9_9DELT1.810e-3053.47Pyridoxine/pyridoxamine 5'-phosphate oxidase n=1 T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig6256contigEcto-sp13_S_contig6256:6957..7638 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeBacteria
Stop1
Start1
Seed ortholog score115.9
Seed ortholog evalue1.1e-23
Seed eggNOG ortholog1121957.ATVL01000009_gene1410
Preferred namepdxH
Model size324
KEGG rclassRC00048,RC00116
KEGG koko:K00275
KEGG ReactionR00277,R00278,R01710,R01711
KEGG Pathwayko00750,ko01100,ko01120,map00750,map01100,map01120
KEGG ModuleM00124
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004733,GO:0006081,GO:0006725,GO:0006732,GO:0006766,GO:0006767,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009108,GO:0009110,GO:0009987,GO:0016491,GO:0016638,GO:0016641,GO:0017144,GO:0018130,GO:0019438,GO:0019637,GO:0034641,GO:0042364,GO:0042816,GO:0042819,GO:0042822,GO:0042823,GO:0044237,GO:0044249,GO:0044271,GO:0044281,GO:0044283,GO:0046184,GO:0046483,GO:0051186,GO:0051188,GO:0055114,GO:0071704,GO:0072524,GO:0072525,GO:0090407,GO:1901360,GO:1901362,GO:1901564,GO:1901566,GO:1901576,GO:1901615,GO:1901617
Exons2
EggNOG free text desc.Catalyzes the oxidation of either pyridoxine 5'- phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP)
EggNOG OGs47P7A@768503,4NFH7@976,COG0259@1,COG0259@2
EC1.4.3.5
Cds size324
COG Functional cat.H
Best tax levelCytophagia
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463869.4565673-CDS-Ecto-sp13_S_contig6256:6956..71131681463869.4565673-CDS-Ecto-sp13_S_contig6256:6956..7113Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig6256 6957..7113 -
1681463869.4693518-CDS-Ecto-sp13_S_contig6256:7471..76381681463869.4693518-CDS-Ecto-sp13_S_contig6256:7471..7638Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig6256 7472..7638 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig6256.16983.1prot_Ecto-sp13_S_contig6256.16983.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig6256 6957..7638 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig6256.16983.1

>prot_Ecto-sp13_S_contig6256.16983.1 ID=prot_Ecto-sp13_S_contig6256.16983.1|Name=mRNA_Ecto-sp13_S_contig6256.16983.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=108bp
MNGVLALQVTEKESTEYFKSRPRGSQVGAWALHQSRPVESRGSLEANVKD
VQVCACSITEVPRPPHFGGFRLRPDRIEFWKGRQTRIHDRIVYVVMDDKW
IMQRLQP*
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mRNA from alignment at Ecto-sp13_S_contig6256:6957..7638-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig6256.16983.1 ID=mRNA_Ecto-sp13_S_contig6256.16983.1|Name=mRNA_Ecto-sp13_S_contig6256.16983.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=682bp|location=Sequence derived from alignment at Ecto-sp13_S_contig6256:6957..7638- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGAACGGTGTTTTGGCTTTGCAGGTGACGGAGAAGGAGAGCACGGAGTA
CTTCAAGAGTCGCCCCCGCGGAAGTCAGGTCGGCGCGTGGGCGCTGCACC
AGAGCCGGCCGGTGGAAAGCCGCGGGTCTCTCGAGGCAAACGTCAAGGAC
GTGCAGGTGTGTGCATGGTGATGATAAATAGAAGCATTTCAGTAACCTTT
GCGAGGTTATCCAATGTCGGTGGTTGTAGGATAGGAACCCCCCCCACCCC
CCTCTCTTTTATTAACAGATTAATGCTAAAAGTTCCTCGCGAGTGTGGTC
CAAGCATGTAGGAGGGAGAAAAAGGTTTGTGAAGCGATTGAGGATATGAT
GTCGATCTATCAAGGGGATGTTAGATTTTCTTCCCCGGCCCCCTCGACCG
CTAGTCAGACCTGTGTGCCCAATATAGCCCTATTGTATGTATCGGCCTAG
TTGACACGGAATAATCCCACCGCCGCATATTCTCACCACCGATCGATCAC
CACAATCACCACAGGACAAGTTCAGCTCGATCACGGAGGTACCGCGGCCG
CCCCACTTCGGAGGGTTCCGTCTCCGGCCTGACCGGATAGAATTCTGGAA
AGGCCGCCAAACACGCATCCACGACCGCATCGTGTACGTCGTTATGGACG
ACAAGTGGATCATGCAGCGTCTACAGCCGTAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig6256:6957..7638-

>mRNA_Ecto-sp13_S_contig6256.16983.1 ID=mRNA_Ecto-sp13_S_contig6256.16983.1|Name=mRNA_Ecto-sp13_S_contig6256.16983.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=324bp|location=Sequence derived from alignment at Ecto-sp13_S_contig6256:6957..7638- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGAACGGTGTTTTGGCTTTGCAGGTGACGGAGAAGGAGAGCACGGAGTA
CTTCAAGAGTCGCCCCCGCGGAAGTCAGGTCGGCGCGTGGGCGCTGCACC
AGAGCCGGCCGGTGGAAAGCCGCGGGTCTCTCGAGGCAAACGTCAAGGAC
GTGCAGGTGTGTGCATGCTCGATCACGGAGGTACCGCGGCCGCCCCACTT
CGGAGGGTTCCGTCTCCGGCCTGACCGGATAGAATTCTGGAAAGGCCGCC
AAACACGCATCCACGACCGCATCGTGTACGTCGTTATGGACGACAAGTGG
ATCATGCAGCGTCTACAGCCGTAG
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