mRNA_Ecto-sp13_S_contig60082.16614.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig60082.16614.1
Unique NamemRNA_Ecto-sp13_S_contig60082.16614.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig60082.16614.1 vs. uniprot
Match: D8LU94_ECTSI (Coronin n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LU94_ECTSI)

HSP 1 Score: 120 bits (301), Expect = 6.050e-31
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 1 
Query:    1 VDENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK 174
            VDENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK
Sbjct:  295 VDENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK 352
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BLAST of mRNA_Ecto-sp13_S_contig60082.16614.1 vs. uniprot
Match: A0A836CNW2_9STRA (Coronin n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CNW2_9STRA)

HSP 1 Score: 112 bits (280), Expect = 7.200e-28
Identity = 51/58 (87.93%), Postives = 58/58 (100.00%), Query Frame = 1 
Query:    1 VDENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK 174
            VDENPWCFAISE+RSTTAAKGMAM+PKRGL+VM+CETARLLKLT++S+EPLSFVVPRK
Sbjct:  295 VDENPWCFAISEYRSTTAAKGMAMVPKRGLDVMRCETARLLKLTTSSVEPLSFVVPRK 352
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BLAST of mRNA_Ecto-sp13_S_contig60082.16614.1 vs. uniprot
Match: A0A835YLY0_9STRA (Coronin n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YLY0_9STRA)

HSP 1 Score: 106 bits (265), Expect = 8.640e-26
Identity = 48/58 (82.76%), Postives = 57/58 (98.28%), Query Frame = 1 
Query:    1 VDENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK 174
            VDENP+CFAISE+RST AAKGMAM+PKRGLN+M+CETARLLKLT++S+EPLSF+VPRK
Sbjct:  295 VDENPFCFAISEYRSTVAAKGMAMVPKRGLNIMQCETARLLKLTTSSVEPLSFMVPRK 352
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BLAST of mRNA_Ecto-sp13_S_contig60082.16614.1 vs. uniprot
Match: A0A7S2CGU3_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2CGU3_9STRA)

HSP 1 Score: 91.7 bits (226), Expect = 2.510e-22
Identity = 43/56 (76.79%), Postives = 49/56 (87.50%), Query Frame = 1 
Query:    7 ENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK 174
            ENP+ F +SE+ STT AKGMA IPKRG NVMKCETAR LKLTSN++EPLSF+VPRK
Sbjct:    1 ENPFLFKLSEYSSTTPAKGMAFIPKRGCNVMKCETARGLKLTSNAVEPLSFIVPRK 56
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BLAST of mRNA_Ecto-sp13_S_contig60082.16614.1 vs. uniprot
Match: A0A7S3XP63_HETAK (Coronin n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XP63_HETAK)

HSP 1 Score: 97.1 bits (240), Expect = 3.020e-22
Identity = 44/58 (75.86%), Postives = 53/58 (91.38%), Query Frame = 1 
Query:    1 VDENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK 174
            VDE P+C  I+EFR+TTAAKGM  +PKRGL+VM+CETARLLKLTS+S+EPLSF+VPRK
Sbjct:  316 VDEAPFCIPINEFRTTTAAKGMCAVPKRGLDVMRCETARLLKLTSSSVEPLSFIVPRK 373
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BLAST of mRNA_Ecto-sp13_S_contig60082.16614.1 vs. uniprot
Match: A0A7S2F3J1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2F3J1_9STRA)

HSP 1 Score: 85.5 bits (210), Expect = 9.420e-20
Identity = 40/58 (68.97%), Postives = 46/58 (79.31%), Query Frame = 1 
Query:    1 VDENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK 174
            V E P  F +SEFRST AAKG+  +PKRGL+V+KCETAR LKLT N IEPL F+VPRK
Sbjct:   34 VTETPCFFRLSEFRSTVAAKGVTFLPKRGLDVLKCETARALKLTGNCIEPLKFIVPRK 91
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BLAST of mRNA_Ecto-sp13_S_contig60082.16614.1 vs. uniprot
Match: W7TD57_9STRA (Coronin n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TD57_9STRA)

HSP 1 Score: 88.6 bits (218), Expect = 3.320e-19
Identity = 39/58 (67.24%), Postives = 51/58 (87.93%), Query Frame = 1 
Query:    1 VDENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK 174
            V E P+ F ISE+RSTT +KG  ++PKRGLNV++CETAR+LKLT++S+EPLSF+VPRK
Sbjct:  295 VSEAPFVFPISEYRSTTPSKGCCLVPKRGLNVLRCETARVLKLTTSSVEPLSFIVPRK 352
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BLAST of mRNA_Ecto-sp13_S_contig60082.16614.1 vs. uniprot
Match: A0A7R9YBR7_9STRA (Coronin (Fragment) n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9YBR7_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 7.230e-19
Identity = 39/57 (68.42%), Postives = 49/57 (85.96%), Query Frame = 1 
Query:    4 DENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK 174
            DENP+ ++ISE+RST + KGM M+PKR  +VM+CETAR LKLTSNS+ PLSF+VPRK
Sbjct:  298 DENPFSYSISEYRSTQSQKGMCMLPKRACDVMRCETARFLKLTSNSVMPLSFIVPRK 354
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BLAST of mRNA_Ecto-sp13_S_contig60082.16614.1 vs. uniprot
Match: A0A7S2XV61_9STRA (Coronin n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XV61_9STRA)

HSP 1 Score: 86.3 bits (212), Expect = 2.140e-18
Identity = 39/58 (67.24%), Postives = 51/58 (87.93%), Query Frame = 1 
Query:    1 VDENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK 174
            V+E+P+ +AI+EFR+  AAKGM M+PK GLNV K ETARLLK+TS+++EPLSF+VPRK
Sbjct:  296 VEESPFVYAINEFRTNNAAKGMGMVPKMGLNVSKNETARLLKMTSSTVEPLSFIVPRK 353
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BLAST of mRNA_Ecto-sp13_S_contig60082.16614.1 vs. uniprot
Match: M4BYQ8_HYAAE (Coronin n=28 Tax=Peronosporaceae TaxID=4777 RepID=M4BYQ8_HYAAE)

HSP 1 Score: 85.9 bits (211), Expect = 3.090e-18
Identity = 40/54 (74.07%), Postives = 47/54 (87.04%), Query Frame = 1 
Query:   13 PWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEPLSFVVPRK 174
            P+ F ISE+RSTT+AKGMAMIPKRG ++M+CET RLLKLTSN+IEPL   VPRK
Sbjct:  300 PFAFPISEYRSTTSAKGMAMIPKRGCDIMRCETTRLLKLTSNAIEPLHVFVPRK 353
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig60082.16614.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LU94_ECTSI6.050e-31100.00Coronin n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LU94... [more]
A0A836CNW2_9STRA7.200e-2887.93Coronin n=1 Tax=Tribonema minus TaxID=303371 RepID... [more]
A0A835YLY0_9STRA8.640e-2682.76Coronin n=1 Tax=Tribonema minus TaxID=303371 RepID... [more]
A0A7S2CGU3_9STRA2.510e-2276.79Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A7S3XP63_HETAK3.020e-2275.86Coronin n=2 Tax=Heterosigma akashiwo TaxID=2829 Re... [more]
A0A7S2F3J1_9STRA9.420e-2068.97Hypothetical protein (Fragment) n=1 Tax=Dictyocha ... [more]
W7TD57_9STRA3.320e-1967.24Coronin n=2 Tax=Monodopsidaceae TaxID=425072 RepID... [more]
A0A7R9YBR7_9STRA7.230e-1968.42Coronin (Fragment) n=1 Tax=Pinguiococcus pyrenoido... [more]
A0A7S2XV61_9STRA2.140e-1867.24Coronin n=1 Tax=Fibrocapsa japonica TaxID=94617 Re... [more]
M4BYQ8_HYAAE3.090e-1874.07Coronin n=28 Tax=Peronosporaceae TaxID=4777 RepID=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig60082contigEcto-sp13_S_contig60082:537..710 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score119.8
Seed ortholog evalue4.3e-25
Seed eggNOG ortholog2880.D8LU94
Preferred nameCORO2A
Model size174
KEGG koko:K13882,ko:K13886,ko:K13887
KEGG Pathwayko04145,ko05152,map04145,map05152
Hectar predicted targeting categoryno signal peptide or anchor
GOsGO:0000147,GO:0000278,GO:0000281,GO:0000910,GO:0001667,GO:0001725,GO:0001755,GO:0001772,GO:0001775,GO:0001776,GO:0001845,GO:0001891,GO:0001894,GO:0001906,GO:0001909,GO:0001932,GO:0001933,GO:0001952,GO:0001953,GO:0002228,GO:0002252,GO:0002260,GO:0002263,GO:0002285,GO:0002323,GO:0002366,GO:0002376,GO:0002443,GO:0002449,GO:0002520,GO:0002521,GO:0002682,GO:0002684,GO:0002694,GO:0002696,GO:0003674,GO:0003779,GO:0003785,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005768,GO:0005769,GO:0005826,GO:0005829,GO:0005856,GO:0005884,GO:0005885,GO:0005886,GO:0005901,GO:0005903,GO:0005911,GO:0005937,GO:0005938,GO:0006469,GO:0006810,GO:0006811,GO:0006812,GO:0006816,GO:0006887,GO:0006897,GO:0006909,GO:0006928,GO:0006935,GO:0006950,GO:0006952,GO:0006955,GO:0006970,GO:0006972,GO:0006996,GO:0007010,GO:0007015,GO:0007017,GO:0007033,GO:0007040,GO:0007049,GO:0007154,GO:0007155,GO:0007162,GO:0007165,GO:0007166,GO:0007275,GO:0007525,GO:0007527,GO:0008017,GO:0008022,GO:0008064,GO:0008092,GO:0008104,GO:0008150,GO:0008152,GO:0008154,GO:0008284,GO:0008360,GO:0009267,GO:0009605,GO:0009607,GO:0009611,GO:0009617,GO:0009620,GO:0009628,GO:0009719,GO:0009888,GO:0009892,GO:0009986,GO:0009987,GO:0009991,GO:0010033,GO:0010324,GO:0010467,GO:0010522,GO:0010563,GO:0010605,GO:0010631,GO:0010632,GO:0010633,GO:0010638,GO:0010639,GO:0010721,GO:0010762,GO:0010769,GO:0010771,GO:0010810,GO:0010812,GO:0010941,GO:0010959,GO:0012505,GO:0012506,GO:0014031,GO:0014032,GO:0014033,GO:0014070,GO:0014910,GO:0014912,GO:0015629,GO:0015631,GO:0016020,GO:0016043,GO:0016050,GO:0016192,GO:0016328,GO:0016477,GO:0016482,GO:0016600,GO:0017016,GO:0017022,GO:0017048,GO:0017053,GO:0019220,GO:0019222,GO:0019899,GO:0022402,GO:0022407,GO:0022409,GO:0022411,GO:0022603,GO:0022604,GO:0022607,GO:0022610,GO:0023052,GO:0030001,GO:0030027,GO:0030029,GO:0030030,GO:0030036,GO:0030041,GO:0030042,GO:0030054,GO:0030097,GO:0030098,GO:0030101,GO:0030139,GO:0030154,GO:0030155,GO:0030217,GO:0030334,GO:0030335,GO:0030336,GO:0030424,GO:0030427,GO:0030479,GO:0030587,GO:0030595,GO:0030659,GO:0030666,GO:0030674,GO:0030832,GO:0030833,GO:0030834,GO:0030835,GO:0030838,GO:0030863,GO:0030864,GO:0030865,GO:0030866,GO:0031090,GO:0031152,GO:0031252,GO:0031267,GO:0031323,GO:0031324,GO:0031334,GO:0031338,GO:0031339,GO:0031344,GO:0031345,GO:0031346,GO:0031399,GO:0031400,GO:0031410,GO:0031529,GO:0031589,GO:0031667,GO:0031668,GO:0031669,GO:0031982,GO:0032036,GO:0032101,GO:0032102,GO:0032153,GO:0032155,GO:0032268,GO:0032269,GO:0032271,GO:0032273,GO:0032420,GO:0032421,GO:0032426,GO:0032432,GO:0032501,GO:0032502,GO:0032506,GO:0032535,GO:0032796,GO:0032879,GO:0032940,GO:0032944,GO:0032946,GO:0032956,GO:0032970,GO:0032984,GO:0032991,GO:0033036,GO:0033043,GO:0033554,GO:0033673,GO:0034097,GO:0034315,GO:0034316,GO:0034613,GO:0034622,GO:0034762,GO:0034765,GO:0035556,GO:0035767,GO:0035838,GO:0036089,GO:0036119,GO:0036120,GO:0038179,GO:0038180,GO:0040011,GO:0040012,GO:0040013,GO:0040017,GO:0042060,GO:0042102,GO:0042110,GO:0042127,GO:0042129,GO:0042221,GO:0042267,GO:0042325,GO:0042326,GO:0042330,GO:0042493,GO:0042592,GO:0042594,GO:0042641,GO:0042742,GO:0042802,GO:0042803,GO:0042981,GO:0042995,GO:0043005,GO:0043029,GO:0043066,GO:0043067,GO:0043069,GO:0043085,GO:0043086,GO:0043087,GO:0043170,GO:0043207,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043232,GO:0043242,GO:0043244,GO:0043254,GO:0043269,GO:0043299,GO:0043320,GO:0043332,GO:0043519,GO:0043521,GO:0043523,GO:0043524,GO:0043542,GO:0043547,GO:0043548,GO:0043549,GO:0043624,GO:0043933,GO:0044085,GO:0044087,GO:0044089,GO:0044092,GO:0044093,GO:0044387,GO:0044396,GO:0044422,GO:0044424,GO:0044425,GO:0044430,GO:0044433,GO:0044444,GO:0044446,GO:0044448,GO:0044459,GO:0044463,GO:0044464,GO:0044764,GO:0044853,GO:0044877,GO:0045055,GO:0045087,GO:0045121,GO:0045184,GO:0045321,GO:0045335,GO:0045472,GO:0045595,GO:0045596,GO:0045785,GO:0045859,GO:0045936,GO:0046649,GO:0046677,GO:0046903,GO:0046907,GO:0046983,GO:0048365,GO:0048468,GO:0048471,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048534,GO:0048583,GO:0048585,GO:0048731,GO:0048762,GO:0048856,GO:0048863,GO:0048864,GO:0048869,GO:0048870,GO:0048871,GO:0048872,GO:0048873,GO:0050670,GO:0050671,GO:0050789,GO:0050790,GO:0050793,GO:0050794,GO:0050832,GO:0050863,GO:0050865,GO:0050867,GO:0050870,GO:0050896,GO:0050900,GO:0050918,GO:0050920,GO:0050922,GO:0051015,GO:0051017,GO:0051020,GO:0051049,GO:0051051,GO:0051093,GO:0051094,GO:0051125,GO:0051126,GO:0051127,GO:0051128,GO:0051129,GO:0051130,GO:0051171,GO:0051172,GO:0051174,GO:0051179,GO:0051234,GO:0051239,GO:0051241,GO:0051246,GO:0051248,GO:0051249,GO:0051251,GO:0051258,GO:0051261,GO:0051270,GO:0051271,GO:0051272,GO:0051279,GO:0051282,GO:0051286,GO:0051301,GO:0051336,GO:0051338,GO:0051345,GO:0051348,GO:0051493,GO:0051494,GO:0051495,GO:0051641,GO:0051649,GO:0051666,GO:0051674,GO:0051703,GO:0051704,GO:0051707,GO:0051716,GO:0051893,GO:0051895,GO:0051924,GO:0060090,GO:0060249,GO:0060255,GO:0060284,GO:0060326,GO:0060341,GO:0060485,GO:0060491,GO:0060548,GO:0060627,GO:0061024,GO:0061061,GO:0061502,GO:0061572,GO:0061640,GO:0061645,GO:0061802,GO:0061836,GO:0065003,GO:0065007,GO:0065008,GO:0065009,GO:0070528,GO:0070663,GO:0070665,GO:0070670,GO:0070727,GO:0070838,GO:0070848,GO:0070887,GO:0070925,GO:0070938,GO:0071310,GO:0071345,GO:0071353,GO:0071363,GO:0071495,GO:0071496,GO:0071671,GO:0071672,GO:0071704,GO:0071840,GO:0071933,GO:0071944,GO:0072511,GO:0080090,GO:0080171,GO:0090066,GO:0090109,GO:0090130,GO:0090132,GO:0090135,GO:0090382,GO:0090630,GO:0090702,GO:0097327,GO:0097435,GO:0097458,GO:0097517,GO:0097708,GO:0098542,GO:0098588,GO:0098589,GO:0098590,GO:0098630,GO:0098657,GO:0098743,GO:0098796,GO:0098797,GO:0098805,GO:0098857,GO:0098858,GO:0098862,GO:0098927,GO:0099024,GO:0099080,GO:0099081,GO:0099120,GO:0099512,GO:0099513,GO:0099568,GO:0099738,GO:0110053,GO:0120025,GO:0120032,GO:0120035,GO:0120036,GO:0120038,GO:1900024,GO:1900025,GO:1900027,GO:1901214,GO:1901215,GO:1901654,GO:1901700,GO:1901879,GO:1901880,GO:1901888,GO:1901889,GO:1902410,GO:1902463,GO:1902743,GO:1902744,GO:1902745,GO:1902903,GO:1902904,GO:1902905,GO:1903037,GO:1903039,GO:1903047,GO:1903169,GO:1903391,GO:1903392,GO:1903673,GO:1904062,GO:1904643,GO:1905861,GO:1990089,GO:1990090,GO:1990819,GO:2000145,GO:2000146,GO:2000147,GO:2000392,GO:2000393,GO:2000394,GO:2000601
Exons1
EggNOG free text desc.actin filament binding
EggNOG OGsCOG1985@1,KOG0303@2759
Cds size174
COG Functional cat.H
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04131,ko04147,ko04812
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463836.9311643-CDS-Ecto-sp13_S_contig60082:536..7101681463836.9311643-CDS-Ecto-sp13_S_contig60082:536..710Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig60082 537..710 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig60082.16614.1prot_Ecto-sp13_S_contig60082.16614.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig60082 537..710 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig60082.16614.1

>prot_Ecto-sp13_S_contig60082.16614.1 ID=prot_Ecto-sp13_S_contig60082.16614.1|Name=mRNA_Ecto-sp13_S_contig60082.16614.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=58bp
VDENPWCFAISEFRSTTAAKGMAMIPKRGLNVMKCETARLLKLTSNSIEP
LSFVVPRK
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mRNA from alignment at Ecto-sp13_S_contig60082:537..710-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig60082.16614.1 ID=mRNA_Ecto-sp13_S_contig60082.16614.1|Name=mRNA_Ecto-sp13_S_contig60082.16614.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=174bp|location=Sequence derived from alignment at Ecto-sp13_S_contig60082:537..710- (Ectocarpus species13 EcNAP12_S_4_19m)
GTGGACGAGAACCCGTGGTGCTTCGCCATCAGCGAGTTCCGGTCGACCAC
GGCGGCGAAGGGGATGGCCATGATCCCCAAGAGGGGCCTCAACGTCATGA
AGTGCGAGACGGCGCGGCTGCTCAAGCTCACGTCCAACTCGATCGAGCCG
CTGAGCTTCGTCGTGCCGAGAAAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig60082:537..710-

>mRNA_Ecto-sp13_S_contig60082.16614.1 ID=mRNA_Ecto-sp13_S_contig60082.16614.1|Name=mRNA_Ecto-sp13_S_contig60082.16614.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=174bp|location=Sequence derived from alignment at Ecto-sp13_S_contig60082:537..710- (Ectocarpus species13 EcNAP12_S_4_19m)
GTGGACGAGAACCCGTGGTGCTTCGCCATCAGCGAGTTCCGGTCGACCAC
GGCGGCGAAGGGGATGGCCATGATCCCCAAGAGGGGCCTCAACGTCATGA
AGTGCGAGACGGCGCGGCTGCTCAAGCTCACGTCCAACTCGATCGAGCCG
CTGAGCTTCGTCGTGCCGAGAAAG
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