mRNA_Ecto-sp13_S_contig6.16584.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

BLAST of mRNA_Ecto-sp13_S_contig6.16584.1 vs. uniprot
Match: D7FPP9_ECTSI (Alanine--tRNA ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FPP9_ECTSI)

HSP 1 Score: 1885 bits (4884), Expect = 0.000e+0
Identity = 957/995 (96.18%), Postives = 963/995 (96.78%), Query Frame = 2

Query:  137 MAEMRVEQWESKQADEVQTDEVQVEFSDNGWPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRESDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAAGGKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAKASFVLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDVGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDVVCKVDYKRRANVAPNHTMTHVLNFALRKVLDPSDGMCDQKGSFLDDEKLRFDFSWSGALTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAKRIQSLRGVFGERYPDPVRVLSIGQDVPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTNKWVQSALDASGRGGKGGGKGDRAQGISQGVDTIDKAMEVVRGPV 3121
            MAEMRV+Q ES            VEFSDNGWPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRE+DGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAI SATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMS             KAAGGKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAK   VLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYD+GELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDVVCKVDY+RRANVAPNHTMTHVLNFALRKVLDPSDGMCDQKGSFLDDEKLRFDFSWSG+LTAEQVADVEDLVNDKINKNLPVYAFVAPLEQA+RIQSLRGVFGERYPDPVRVLSIGQDVPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTNKWVQ ALDASGRGGKGGGKGDRAQGISQGVDTIDKAMEV RGPV
Sbjct:    1 MAEMRVKQSESXXXXXXXXXXXXVEFSDNGWPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRENDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAIRSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSDEKTKSNQARSNQKAAGGKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAK---VLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDIGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDVVCKVDYERRANVAPNHTMTHVLNFALRKVLDPSDGMCDQKGSFLDDEKLRFDFSWSGSLTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAQRIQSLRGVFGERYPDPVRVLSIGQDVPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTNKWVQCALDASGRGGKGGGKGDRAQGISQGVDTIDKAMEVARGPV 992          

BLAST of mRNA_Ecto-sp13_S_contig6.16584.1 vs. uniprot
Match: A0A6H5L0C8_9PHAE (Alanine--tRNA ligase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L0C8_9PHAE)

HSP 1 Score: 1360 bits (3519), Expect = 0.000e+0
Identity = 735/855 (85.96%), Postives = 739/855 (86.43%), Query Frame = 2

Query:  353 MNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRESDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAAGGKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAKASFVLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDVGELSLSDGSGSFAVRQVSSFGAYTLHIGQ------VKAGTLSVGSDVVCKVDYKRRANVAPNHTMTHVLNFALRKVLDPSDGMCDQKGSFLDDEKLRFDFSWSGALTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAKRIQSLRGVFGERYPDPVRVLSIGQDVPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTS 2899
            MNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIE                                       GKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDSKVFPGT AFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAAGGKPMILEAEQ                              VLALFVGKGEGEDGVGFVPSASAV DEADQQTVGVVLDCTSFYAEGGGQIYD+GELSLSDGSGSFAVRQVS+FGAYTLHIGQ      VKAGTLSVGSDVVCKVDY+RRANVAPNHTMTHVLNFALRKV                                   ADVEDLVNDKINKNLPVYAFVAPLEQA+RIQSLRGVFGERYPDPVRVLSIGQDVPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDT VRRLGAELDGAVISVK KNKLRARLATLVQKAKALKKKVMEERVGQAVT GKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPEL S
Sbjct:    1 MNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIE---------------------------------------GKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDSKVFPGTVAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAAGGKPMILEAEQ------------------------------VLALFVGKGEGEDGVGFVPSASAVVDEADQQTVGVVLDCTSFYAEGGGQIYDIGELSLSDGSGSFAVRQVSNFGAYTLHIGQYYGAVEVKAGTLSVGSDVVCKVDYERRANVAPNHTMTHVLNFALRKV-----------------------------------ADVEDLVNDKINKNLPVYAFVAPLEQAQRIQSLRGVFGERYPDPVRVLSIGQDVPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTKVRRLGAELDGAVISVKAKNKLRARLATLVQKAKALKKKVMEERVGQAVTAGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELVS 751          

BLAST of mRNA_Ecto-sp13_S_contig6.16584.1 vs. uniprot
Match: A0A6V2XN65_HETAK (Alanine--tRNA ligase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V2XN65_HETAK)

HSP 1 Score: 1117 bits (2889), Expect = 0.000e+0
Identity = 574/966 (59.42%), Postives = 706/966 (73.08%), Query Frame = 2

Query:  227 WPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRESDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAAL-GDSK-VFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAAGGKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAKASFVLALFVGKGEGEDG-VGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDVGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAG-TLSVGSDVVCKVDYKRRANVAPNHTMTHVLNFALRKVL-----DPSDGMCDQKGSFLDDEKLRFDFSWSGALTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAKRIQSLRGVFGERYPDPVRVLSIGQDVPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTNKWVQSALDASGRGGKGGGKGDRAQGISQGVDTIDKA 3097
            WPA R+R+SFI+FF ++++H FW SSPVVPHDDPTLLFANAGMNQFKP FLGT DP+LP++KL+RA NSQKCIRAGGKHNDLDDVGKD+YHHTFFEMLGNWSFG+YFK EAI +AWECLT V+ L P RLYATYFGGDE   +  DE+A++IWL++LP  RVLPF   DNFWEMGD GPCGPC EIHYDRIGGRDAA  VN D P+VIEIWN VFIQ+NRE  G L+ELPNKH+DTG+GFERLASILQGK SNYDTD+F PIF AI   TGAR YTGK+  ED+DN+DMAYRVVADHIRTLT AITDG  PS+DGRGYVLRRVLRR +RYGQQVLGG PGFFH LVPVVVGLMKG FPEL   +A V  VIK+EE SFNKTLDKGL+EFNK+A  +  + K VFPG AA  LY++MGFP+DL TLMAEE+GL++D EGF+ KM              K+ GG  ++LEAEQ  WL+     AT+++ +Y   ++ A   + V+A+F  K E ++G  GFV SA+A         VGV+LD T+FYAE GGQI+D G ++  +   +F V    +F  Y LH+G+V  G +L+VG+ V   VDY RRA VAPNHTMTHVLNFALR+VL     D   G CDQKGS +D E+LRFDF+W+G L  EQ+A VE +VN+KI + LPVYA V+P EQA +I SLR VFGERYPDPVRV+S+G  V  +L DP +  W+ +S+EFCGGTHL+NT +A  F ++EE+GIAKG+RR++AVTRG A  A +R++AFE +L  A  +E  +L    + L   L+   ISV  KN+LR  L  L  K KA KKK ++   G A+ + + +A++ +EAG  ++V R DFGCD K   K + E+ K  PE   M+L +ADE+ADR+ C  +IP +K    D N W+ +A+DA+G GGK GGK D A G   G   ID A
Sbjct:   18 WPAERIRESFIAFFRDRYEHVFWPSSPVVPHDDPTLLFANAGMNQFKPHFLGTMDPSLPMAKLSRAVNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGAYFKREAIAWAWECLTEVFGLSPERLYATYFGGDETQGLEPDEEAREIWLQFLPAERVLPFGCKDNFWEMGDTGPCGPCTEIHYDRIGGRDAAPLVNADLPDVIEIWNNVFIQFNREEGGVLRELPNKHVDTGMGFERLASILQGKTSNYDTDIFQPIFRAIQQVTGARDYTGKIEAEDTDNVDMAYRVVADHIRTLTFAITDGAVPSSDGRGYVLRRVLRRGVRYGQQVLGGPPGFFHQLVPVVVGLMKGAFPELEGRQAFVMDVIKEEEESFNKTLDKGLREFNKIAEQVKAEGKTVFPGDAAQFLYATMGFPVDLTTLMAEEQGLTLDAEGFEAKMEEEREKSKEAHAAAKSGGGANLVLEAEQTSWLANNGVAATDSAPKYLGAEEGAGLPAVVVAIFK-KSEVDNGRAGFVDSATAA-----DGPVGVLLDRTAFYAESGGQIFDTGAIAFGEAGEAFEVGNCQTFAGYDLHVGEVAGGGSLAVGAAVRVGVDYARRARVAPNHTMTHVLNFALREVLLGGVTDDRTGACDQKGSAVDAERLRFDFAWNGPLEPEQLARVEAIVNEKIAEQLPVYAEVSPFEQACQIPSLRRVFGERYPDPVRVISVGAPVGELLADPLDPKWNGLSIEFCGGTHLTNTGQAAFFQLMEESGIAKGIRRVIAVTRGAAEEARDRSRAFEAELKAAAELEGEALLEACKGLTQALNPLAISVVDKNRLREELGALATKVKAWKKKQLKAMSGMALGLCEEKAKEAREAGARKVVLRVDFGCDGKVGKKCVQEMHKHHPEGVFMVL-SADEDADRFQCYAMIPGAKEKGLDANAWLAAAMDAAG-GGKSGGKPDNAMGTVNGCSGIDAA 975          

BLAST of mRNA_Ecto-sp13_S_contig6.16584.1 vs. uniprot
Match: A0A835Z4U5_9STRA (Alanine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z4U5_9STRA)

HSP 1 Score: 1105 bits (2858), Expect = 0.000e+0
Identity = 569/984 (57.83%), Postives = 692/984 (70.33%), Query Frame = 2

Query:  221 NGWPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRESDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAA---ALGDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAAGGKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAKASFVLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDVGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDV-----------VCKVDYKRRANVAPNHTMTHVLNFALRKVL-------DPSDGMCDQKGSFLDDEKLRFDFSWSGALTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAKRIQSLRGVFGERYPDPVRVLSIGQDVPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGI---EDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTNKWVQSALDASGRGGKGGGKGDRAQGISQGVDTIDKAM 3100
            NGWPA R+R +FI FF +K  HTFW SSPVVPHDDPTLLFANAGMNQFKPLFLGT DP+LPLSKLTRA NSQKCIRAGGKHNDLDDVGKD+YHHTFFEMLGNWSFG YFK+EAI +AWECLT  + L+  RLYATYFGGD  L +  DE+AK IWLKYLP  RVLPF   DNFWEMGD GPCGPC EIHYDRIGGRDAA  VN D+P+VIEIWN VFIQ+NRES G LKELP+KH+DTG+GFERLASILQGK SNYDTDVF+PIF  I S TGARPY+GKVGEED+D +DMAYRVVADHIRTLT AITDG  PSNDGRGYVLRR+LRR +RYGQQ+LG KPGFFH LVPVVV ++KG FPEL +  + V  ++KDEE +FN+TLDKGL+EF+K A    A G  KVFPGT AF LYS+MGFP+DL  LMAEE GL+VD+  +  KM              +AAGG  + +EAEQ   L+ +    TE+  +YTWH  P+AK   V+AL+ GKG+G  G GFV  A       + + VG++LD T FYAE GGQ+YD G L   D S    V  V ++G Y LH G V    L+VG+ V            C VDY RR+ VAPNHTMTHVLN+ALR VL         + G+CDQKGSF+DD+KLRFDF+WSG L A+Q+A VE +VN+ I   +PVYA VAPL++A +I++LR VFGERYPDPVRVLS+GQ+VPAMLEDP++ +WS++SVEFCGGTHL+NT +AE F I+EE GIAKG+RRI+AVTR  A  A   A  FE+++     +   + + L+   +RL   LD A IS   K ++R  +A       A KKK   E V  A  +G    E  K AG  +++     G D K+    L     A  ++ +++ F +D +A RY C  ++P +  A K    W+++AL + G G   GGKGD+A G   G++ +  A+
Sbjct:   16 NGWPAERIRKTFIEFFEQKQ-HTFWPSSPVVPHDDPTLLFANAGMNQFKPLFLGTVDPSLPLSKLTRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKQEAITWAWECLTVTFGLNAERLYATYFGGDPALGLAPDEEAKAIWLKYLPAERVLPFGCKDNFWEMGDTGPCGPCTEIHYDRIGGRDAAVLVNADRPDVIEIWNNVFIQFNRESGGVLKELPSKHVDTGMGFERLASILQGKDSNYDTDVFMPIFGVIQSVTGARPYSGKVGEEDTDTVDMAYRVVADHIRTLTFAITDGAVPSNDGRGYVLRRILRRGVRYGQQILGAKPGFFHQLVPVVVNMLKGAFPELKNRVSFVMDILKDEETAFNRTLDKGLREFSKAATKVKATGGPKVFPGTTAFLLYSAMGFPVDLTQLMAEEAGLTVDMVEYHAKMEEDKAKSAAAEVARRAAGGTALTMEAEQTSALAAKGVAGTESEAKYTWHHTPSAK---VVALYAGKGKGALGDGFVEEA------VEGERVGIILDTTPFYAEQGGQVYDTGSLDAEDLS--VEVVNVQTYGGYVLHTGIVSGKALAVGTTVXXXXXXXXXXXTCSVDYDRRSRVAPNHTMTHVLNYALRSVLCGGLDNKSVAQGLCDQKGSFVDDDKLRFDFAWSGPLPADQIAAVEKIVNETIQAKMPVYAHVAPLDKALKIRALRSVFGERYPDPVRVLSVGQEVPAMLEDPDSDSWSNLSVEFCGGTHLTNTGDAEAFAIMEETGIAKGIRRIIAVTRERAHEAKALAGTFEEEVKALAPLATQDPLKLEKETKRLLPVLDTAAISATDKQRIRDIIAGYTVAITAHKKKAANEGVAAAKELGTTAGEAAKAAGKDRVIVVLPDGADNKTITAALTAAKAAHGDVKALLGFASDPDAGRYACVAVMPGTSGAAK---AWLEAALSSVG-GTASGGKGDKAVGFGSGIENVAAAI 983          

BLAST of mRNA_Ecto-sp13_S_contig6.16584.1 vs. uniprot
Match: W7TIB5_9STRA (Alanine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TIB5_9STRA)

HSP 1 Score: 1046 bits (2705), Expect = 0.000e+0
Identity = 536/980 (54.69%), Postives = 667/980 (68.06%), Query Frame = 2

Query:  218 DNGWPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRESDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAAL-----------GDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAAGGKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAKASFVLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDVGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDVVCKVDYKRRANVAPNHTMTHVLNFALRKVL----DPSDGMCDQKGSFLDDEKLRFDFSWSGALTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAKRIQSLRGVFGERYPDPVRVLSIGQDVPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTNKWVQSALDASGRGGKGGGKGDRAQGISQGVDTIDKAMEVVR 3112
            +  W A RVR +FI +FT K +H FW SSPVVPHDDPTLLFANAGMNQ+KPLFLG  DP LPLSKLTRA NSQKCIRAGGKHNDL+DVGKD YHHTFFEMLGNWSFGSYFKEEAI +AW+CL   + LDPSRLYATYFGG+EK  + ADE+A+ IWL+YLP +RVLPF   DNFWEMG+ GPCGPC EIHYDR+  R+  + VNMD P++IEIWNVVFIQYNRE  G+L+ELP+KH+DTG+GFERL SILQ K SNYDTD+F+P+F+AI   TGA PY GK+G+ D ++ DMAYRVVADHIRTLT AI DG  PSNDGRGYVLRR+LRRA+RYG +VLG  PGFF  LV VVV  MK IFPEL + E RV  V+ DEE +FN+TLD+G K F KVA  L           G+   FP   A  LYS+MGFP+DL  LMAEEKGL+VD  GF+  ++             K  GGK ++LEAEQ  WL  R  E T++S +Y WH +P+A+   V+AL+ GK      VGFV   +A     +   V V+L+ TSFYAE GGQI+D G ++  DG+ +  V  V S+G Y LHIG +++G L VG    C VDY+RRA +APNHTMTHVLN+ALR+VL        G+CDQKGS +DDEKLRFDF+W+ ALT E++A+VE +V ++I++ LPV++++APLE+A  I SLR VFGERYPDPVRVLS+G +VP MLEDP N AW D SVEFCGGTHLSNT+EAE F ++EE GIAKG+RRI AVT+ +A AA E A+ F   L +A+   +  L+  V+ L   L    IS   K + R  L  L+ + K  K                                R DFG D K A + L + S+  P     +L +ADE++ ++      P    +  D   WV +A++ +G GG+ GGK  R QG+ +G++ I  A+   +
Sbjct:   23 ETAWSARRVRQTFIDYFTNKREHVFWPSSPVVPHDDPTLLFANAGMNQYKPLFLGIVDPKLPLSKLTRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWDCLVNEFGLDPSRLYATYFGGNEKQGLTADEEARNIWLRYLPPNRVLPFDCKDNFWEMGETGPCGPCTEIHYDRLPAREVPELVNMDSPDLIEIWNVVFIQYNREEGGALRELPSKHVDTGMGFERLTSILQDKPSNYDTDIFMPLFDAIQELTGAPPYAGKLGKADPESRDMAYRVVADHIRTLTFAIADGAVPSNDGRGYVLRRILRRAVRYGAKVLGAAPGFFSKLVHVVVNQMKDIFPELEEKEKRVVEVLADEERAFNRTLDQGEKHFKKVADELLLAASQRGGDKGEKVAFPAKEAHYLYSTMGFPVDLTELMAEEKGLTVDRAGFETLLAEDKRQSAMAAAAKKEMGGKEIVLEAEQTAWLQSRGVEITDSSLKYVWHHEPSAR---VMALYKGKA-----VGFVEEVTA-----EDGVVAVILNTTSFYAEQGGQIHDTGVITNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYERRARIAPNHTMTHVLNYALRRVLLKGVSDGSGLCDQKGSAVDDEKLRFDFAWNKALTKEELAEVETIVRERISQELPVHSYIAPLEKALHINSLRQVFGERYPDPVRVLSVGHEVPPMLEDPTNPAWQDTSVEFCGGTHLSNTREAERFALIEETGIAKGIRRITAVTKDMARAAEELAEEFAGLLALAEVKPEEELEGEVKMLNTRLGPLTISAVEKERFRTALDALLTRVKKFKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERLDFGTDGKIAKEALDKASQIHPS-GCFLLISADEDSGKFGVYSTAPKL-GSPLDPIAWVAAAVEGAG-GGRSGGKSPRGQGVGEGLEKIGSAVTAAK 986          

BLAST of mRNA_Ecto-sp13_S_contig6.16584.1 vs. uniprot
Match: A0A7S4ME88_9STRA (Alanine--tRNA ligase n=2 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4ME88_9STRA)

HSP 1 Score: 1038 bits (2684), Expect = 0.000e+0
Identity = 550/970 (56.70%), Postives = 666/970 (68.66%), Query Frame = 2

Query:  227 WPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRESDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAA-GGKPMILEAEQMDWLSKRDTEATEASGRYTWHQQ-PAAKASFVLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDVGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDVVCKVDYKRRANVAPNHTMTHVLNFALRKVL----DPSDGM-CDQKGSFLDDEKLRFDFSWSGALTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAKRIQSLRGVFGERYPDPVRVLSIGQD-VPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTNKWVQSALDASGRGGKGGGKGDRAQGISQGVDTIDKAMEVVR 3112
            WP  RVR +FI FF +KH HTFW SSP VPHDDPTLLFANAGMNQ+KPLFLGT DP L LSKL RA NSQKCIRAGGKHNDLDDVGKD+YHHT FEMLGNWSFG YFKE AI  AW+CLT  +KLD SRLYATYF GD +  V  D++A+KIWLKYLP  RV+P    DNFWEMG VGPCGPC EIHYDRIGGRDAA  VN D P+VIEIWN VFIQYNRE+DGSL+ LP +H+DTG+GFERL SILQGK SNYDTD+F+P+F AI   TGA  Y+GKVG ED    DMAYRVVADHIRTL+ AI DG TPSNDGRGYVLRRVLRRA+RYG+Q LG + GFF TLVP +V LM G FPEL   +A VTAVIKDEE SF+KTLDKGL  F ++A  +GD KVFPG  A  LY+SMGFP+DL  LMAEE GL++D EGFD KM                  GGK M L AEQ  +L  +  +AT+   +Y W++     KA+   ALFVG+GE EDG+GF  S +      +   VG++LD TSFYAE GGQ YD G +  + G+    +  V S+G + LH+GQV  GT+++G +++C VDY RR  +A NHTMTHVLN+ALR VL    D   G   DQKGS +D+ KLRFDFSW+G L   Q+A VED++N +I+  +P+ +FVAPLE AK+I SLR VFGE YPDPVRV+S+  + +P +L++P++  W   SVEFCGGTHLSNT+EAE FV+L+E GIAKGVRRI AVT G A AA ERA  FE +L  A  +    L+  V++L  EL    ISV  K+  +  L  L +KA A KK+    R  + V    + A    EAGG ++  R DFG D K A  +    +K   +  + +L TADE + R++     P       D   W Q+A +  G G KGGGK D AQ  + G++ I+  +E  R
Sbjct:   13 WPMDRVRSTFIDFFAKKHGHTFWPSSPCVPHDDPTLLFANAGMNQYKPLFLGTCDPNLELSKLKRAVNSQKCIRAGGKHNDLDDVGKDVYHHTMFEMLGNWSFGDYFKEGAIDMAWQCLTEEFKLDTSRLYATYFAGDSQTPV--DDEARKIWLKYLPPERVIPCGAEDNFWEMGAVGPCGPCTEIHYDRIGGRDAAALVNADLPDVIEIWNNVFIQYNREADGSLRPLPAQHVDTGMGFERLTSILQGKDSNYDTDIFMPLFAAIQKLTGAPAYSGKVGAEDEGFRDMAYRVVADHIRTLSFAIADGATPSNDGRGYVLRRVLRRAVRYGRQNLGAELGFFSTLVPTLVDLMGGTFPELKKHQAHVTAVIKDEEESFSKTLDKGLLRFKELADRVGDDKVFPGADAHFLYTSMGFPVDLTELMAEELGLTLDREGFDAKMKDEQDKSAEAHRLKMMGKGGKDMRLVAEQTAYLVNKGVDATDDEAKYVWNKDLEGCKAA---ALFVGRGETEDGIGFADSITG-----ENGAVGLILDKTSFYAEAGGQTYDTGVIKTAGGA-VVRIDNVQSYGQFVLHVGQVTEGTVALGEELICSVDYVRRTPIASNHTMTHVLNYALRDVLVTKGDNKTGQDVDQKGSLVDELKLRFDFSWNGPLKPAQLAAVEDMINSRISAGVPIQSFVAPLEDAKQISSLRAVFGEVYPDPVRVVSVSPEPIPEILQNPQDGQWLQYSVEFCGGTHLSNTKEAEMFVLLKEEGIAKGVRRITAVTGGDAKAAGERADEFEGRLKEAGALPGAELEAGVKKLSEELKDLSISVARKDGFKTELGKLTKKAMAWKKEQAAARTKEVVEQLVSAA---NEAGGNKIALRIDFGIDGKVAKSVAQSFAKKVKD-KAFLLITADESSGRFMVVASAPKGMKDPVDCKAWCQAATE--GTGAKGGGKKDLAQFNATGIEKIEGVLEKAR 965          

BLAST of mRNA_Ecto-sp13_S_contig6.16584.1 vs. uniprot
Match: B7G5M3_PHATC (Alanine--tRNA ligase n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7G5M3_PHATC)

HSP 1 Score: 1022 bits (2642), Expect = 0.000e+0
Identity = 535/975 (54.87%), Postives = 677/975 (69.44%), Query Frame = 2

Query:  227 WPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRESDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAAG-GKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAKASFVLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDVGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDVVCKVDYKRRANVAPNHTMTHVLNFALRKVL---------DPSDGMCDQKGSFLDDEKLRFDFSWSGALTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAKRIQSLRGVFGERYPDPVRVLSIGQD-VPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTN--KWVQSALDASGRGGKGGGKGDRAQGISQGVDTIDKAMEVVR 3112
            WP  +VRD+F ++F E+H H FW SSP VP DDPTLLF NAGMNQ+KPLFLGT DP L +SKLTRA NSQKCIRAGGKHNDLDDVGKD+YHHTFFEMLGNWSFG YFK  AI  AW+CLT  + LDP RLYATYF GDE   V  DE+A+++WL+YLP+ RVLPF   DNFWEMG  GPCGPC EIHYDRIGGRDA+K VN D P+V+EIWNVVFIQYNRE+DGSL+ LP +H+DTG+GFERL SILQ   SNYDTD+F+P+F AI + TGARPY  KVG+ED + +DMAYRVVADHIRTL  AITDG  PSNDGRGYVLRRVLRRA+RYG+Q LG + GFF  LVP  V +M   FPE+V+ +  VT +I++EE SF++TLDKGL++FN++A  +G  ++F G  A  LY+SMGFP+DL  LMAEEKG++++ E F+ KM               A G GK M L AEQ  +L  ++  AT+ + +Y WH++ A     V A F+G+ E ED +GFV S S      +   VG+VLD +SFYAE GGQ+YDVG L+ S G+    +  V ++G + LH+G+V +GTLSVG  V C VDY RRA +A NHTMTHVLN ALR+VL           S    DQKGS +D+ KLRFDFSWSG LT EQ+A+VE L  D+I   +PV A+VAPL  A++I SLR VFGE+YPDPVRV+++    VP ML +P++S W++ SVEFCGGTHL+NT+EAE FV+L E GIAKG+RRI A+T G A  A+  A  FE +L  A+ ++   L++TV++L AELDG  I+   K + R +LAT+ ++  A KK+                    K AGG+++V R DFG + K A  ++    K   +  +++L TAD EADR++   +I  +  A KD N   W+++A D  G   KGGGK D AQ    GV+ +D  +E  R
Sbjct:   15 WPMEKVRDTFKNYFVEQHGHVFWPSSPCVPVDDPTLLFTNAGMNQYKPLFLGTCDPNLEMSKLTRAVNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKAGAIDMAWQCLTVTFGLDPERLYATYFAGDELTPV--DEEARQLWLRYLPDDRVLPFDARDNFWEMGATGPCGPCTEIHYDRIGGRDASKLVNADLPDVMEIWNVVFIQYNREADGSLRPLPAQHVDTGMGFERLTSILQNVDSNYDTDIFIPLFTAIQNITGARPYAKKVGKEDPEYIDMAYRVVADHIRTLCFAITDGAVPSNDGRGYVLRRVLRRAVRYGRQNLGAELGFFAKLVPAFVDVMGSAFPEVVEKQEYVTGIIQEEEESFSRTLDKGLQKFNELAEKVGADQIFSGADAHFLYTSMGFPVDLTELMAEEKGMTLNKEEFEAKMQEEHDISQAAHLAKMAGGSGKDMRLVAEQTSYLVGQNISATDDAAKYVWHEELAD--CVVKACFIGRNETEDMIGFVGSISP-----ESSAVGIVLDKSSFYAEAGGQVYDVGTLTSSTGA-VVKITNVQAYGQFVLHLGEVASGTLSVGDTVKCSVDYVRRAPIASNHTMTHVLNHALREVLIKRPEKESGKTSTLTVDQKGSLVDETKLRFDFSWSGQLTPEQLAEVEKLCMDRIVNAVPVDAYVAPLGDAQQISSLRAVFGEKYPDPVRVVAVSDHAVPEMLANPQDSQWNEYSVEFCGGTHLTNTKEAEAFVLLAEEGIAKGIRRITAITTGEAKKAIALANEFESKLTAAEVVQGDDLESTVKQLSAELDGLDIAAVKKMQFREQLATMTKQVLAYKKQ--------------------KLAGGSKVVMRFDFGVEGKVAKSVMTAFGKQVKD-KALLLVTADPEADRFM---VIAGAPKAMKDLNCKAWIEAATD--GLDAKGGGKPDSAQYQVSGVEAVDTVLEKAR 953          

BLAST of mRNA_Ecto-sp13_S_contig6.16584.1 vs. uniprot
Match: B8C356_THAPS (Alanine--tRNA ligase n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8C356_THAPS)

HSP 1 Score: 1008 bits (2607), Expect = 0.000e+0
Identity = 530/971 (54.58%), Postives = 658/971 (67.77%), Query Frame = 2

Query:  227 WPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRESDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAAG-GKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAKASFVLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDVGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDVVCKVDYKRRANVAPNHTMTHVLNFALRKVL------DPSDGMCDQKGSFLDDEKLRFDFSWSGALTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAKRIQSLRGVFGERYPDPVRVLSIGQD-VPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTNKWVQSALDASGRGGKGGGKGDRAQGISQGVDTIDKAMEVVRG 3115
            WP  +VR +FIS+F E++ HTFW+SSP VPHDDPTLLF NAGMNQ+KPLFLGT DPAL ++ L RATNSQKCIRAGGKHNDLDDVGKD+YHHTFFEMLGNWSFG YFK+EAI  AW+CLT  +KLDP+RLYATYFGGDE      D++A++IWL+YLP  RVLPF   DNFWEMG  GPCGPC EIHYDRIG RDA+K VN D P+VIEIWN VFIQ+NRE+DGSL+ LP +H+DTG+GFERL SILQG  SNYDTD+F+P+F+AI   TGARPY GK+ +ED + +DMAYRVVADH+RTL+ AI DG  PSNDGRGYVLRRVLRRA+RYG+Q L  + GFF  LV  +V LM   FPEL + E  VTA+I++EE SF+KT+DKG+ EFNK   ALGD KVFPG  A  LY+SMGFP+DL  LM EE+GL++D+EG+D KM               +AG GK M L AEQ   L  +  +AT    +Y W++    + S + ALF+G+ E EDG+GFV S S      +   VG+VLD T+FY E GGQ +D G +  S G+ +  +  V  +G++ LHIG V  GT++V     C VDY RRA +A NHTMTH+LNFALR VL      D      DQKGS +D+ KLRFDFSWSG LT EQ+  VE +  D+IN  +PV  +VAPLE A++I SLR VFGE YPDPVRV+++    + +MLE+P+++ W+  SVEFCGGTHL NT EA+ FV+L E GIAKGVRRI  VT   A  A   A  FE +L  A  +E + L+  V+ L  E++G  IS   K + R  + T  +K  A KK    E   Q  TV +   E      G ++V R DFG D K A  +     K   +  +++  TAD +ADR++     P  K +  D   WV +A+  +G  GKGGGK D AQ    GV  ID  +E  RG
Sbjct:   14 WPMDKVRSTFISYF-ERNGHTFWRSSPCVPHDDPTLLFTNAGMNQYKPLFLGTCDPALKMASLKRATNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEAIEMAWKCLTEEFKLDPNRLYATYFGGDES--TPCDDEAREIWLQYLPAERVLPFDAKDNFWEMGATGPCGPCTEIHYDRIGNRDASKLVNADLPDVIEIWNNVFIQFNREADGSLRPLPAQHVDTGMGFERLTSILQGVDSNYDTDIFIPLFDAIQKITGARPYAGKIEKEDPEFIDMAYRVVADHVRTLSFAIADGAVPSNDGRGYVLRRVLRRAVRYGRQNLNAELGFFAQLVRTLVELMGDTFPELREKEEYVTAIIREEEESFSKTVDKGIVEFNKRCDALGDVKVFPGEDAHFLYTSMGFPVDLTELMCEERGLTLDMEGYDAKMKEEQELSNAAHLAKMSAGSGKDMRLVAEQTSHLVNKGVDATNDDAKYVWNED--LEGSIIKALFIGRNETEDGIGFVDSISK-----ENGAVGIVLDKTNFYGESGGQTFDTGSIKTSSGA-TIEITNVQIYGSFVLHIGDVTEGTVTVNDAATCCVDYVRRAPIAANHTMTHILNFALRDVLVTRGDADQHGASVDQKGSLVDENKLRFDFSWSGPLTPEQLVAVEKICVDQINSAVPVDNYVAPLEGAQQISSLRAVFGEVYPDPVRVVAVSPSPITSMLENPQDAQWNKYSVEFCGGTHLKNTSEAQGFVLLSEEGIAKGVRRITGVTMKDAAIATAAASEFEAKLTAAGSLEGLDLEAHVKVLTVEMEGLSISAAKKIQFRDVMGTYGKKVAAWKKANAAE---QTATVVEQVIEAANSTEGNKVVVRCDFGVDGKVAKAVSTAYGKKIKD-KALLFVTADVDADRFMVVAFAP--KGSDVDCKAWVAAAV--AGTDGKGGGKKDSAQSTVPGVGNIDGVLEKARG 965          

BLAST of mRNA_Ecto-sp13_S_contig6.16584.1 vs. uniprot
Match: A0A7S3L1R5_9STRA (Alanine--tRNA ligase n=1 Tax=Amphora coffeiformis TaxID=265554 RepID=A0A7S3L1R5_9STRA)

HSP 1 Score: 999 bits (2583), Expect = 0.000e+0
Identity = 529/963 (54.93%), Postives = 656/963 (68.12%), Query Frame = 2

Query:  227 WPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRESDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAAG-GKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAKASFVLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDVGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDVVCKVDYKRRANVAPNHTMTHVLNFALRKVLDPSDGMC--DQKGSFLDDEKLRFDFSWSGALTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAKRIQSLRGVFGERYPDPVRVLSIGQD-VPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKK-DTNKWVQSALDASGRGGKGGGKGDRAQGISQGVDTIDKAM 3100
            WP  +VR +FI FF  KH H FW SSP VP DDPTLLF NAGMNQ+KPLFLGT DP L +SKL RA NSQKCIRAGGKHNDLDDVGKD+YHHTFFEMLGNWSFG YFK+EAI  AWECL   +++DP+RLYATYF GDE   V  DE+A++IWL+YLP  RVLPF   DNFWEMG  GPCGPC EIHYDRIGGRDAAK VN D P+VIEIWN VFIQYNRE+DGSL+ LP +H+DTG+GFERL SILQG  SNYDTD+F+P+F+AI   TG RPY GKVG ED+D +DMAYRVVADHIRTL  AITDG  PSNDGRGYVLRRVLRRA+RYG+Q LG + GFF  LVP VV L+   FPEL + E +VTA+I+DEE SF++TLDKGL +F ++A  +GD KVF    A  LY++MGFP+DL  LMAEEKGL VD +GF++KM              ++ G GK M L AEQ   L  +  EAT    +Y W+Q+       V ALF+G+ E ED +GFV   S      +  TVGV+LD TSFYAE GGQ+YD G +  + G+    V  V  +G Y LH+G+V +GT   G  V C VDY RR  +A NHTMTHVLNFALRKVL  SDG    DQKGSF D+ K RFDFSW+G +T EQ+A+VE L  ++I K +PV ++VAPL +A+ I SLR VFGE+YPDPVRV+++    +P ML +P+++ W++ +VEFCGGTHL NT+EAE F +L E GIAKG+RRIV VT+  A  A+  A  FE +L+ A  ++   L+   + L  E+ G   S   K++ R  ++ L +K  A KK+ +     + V    A A +T    G +++FR DFG D K    + +   K   +  ++++ +ADE+ D+ L     P  K AK  D   W+ +A    G  GKGGGK D AQ    GV   D  +
Sbjct:   14 WPMKKVRATFIEFFQRKHGHDFWPSSPCVPVDDPTLLFTNAGMNQYKPLFLGTCDPNLEMSKLKRAVNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEAIDMAWECLVHEFQMDPNRLYATYFAGDETTPV--DEEARQIWLQYLPPERVLPFDAKDNFWEMGATGPCGPCTEIHYDRIGGRDAAKLVNADLPDVIEIWNNVFIQYNREADGSLRPLPAQHVDTGMGFERLTSILQGVDSNYDTDIFMPLFKAIQRVTGCRPYGGKVGAEDADFVDMAYRVVADHIRTLCFAITDGAVPSNDGRGYVLRRVLRRAVRYGRQNLGAELGFFAQLVPTVVELLSDTFPELREKEDQVTAIIRDEEESFSRTLDKGLLKFKELAEKVGDDKVFSADDAHFLYTTMGFPIDLTELMAEEKGLKVDKDGFERKMQQEKEISDAARLAKQSGGSGKDMRLVAEQTAHLVTKGVEATNDEPKYVWNQE--LTGCTVKALFIGRNETEDKIGFVDEISP-----ESSTVGVILDKTSFYAEAGGQVYDTGFIKSASGA-QIKVENVQMYGQYVLHLGEVVSGTFKTGETVSCSVDYVRRGPIAANHTMTHVLNFALRKVL-ASDGSITIDQKGSFQDEFKSRFDFSWNGQVTTEQLAEVEKLCIERIEKEVPVESYVAPLAKAQEISSLRAVFGEKYPDPVRVVAVSDHTIPEMLGNPQDATWNEYAVEFCGGTHLKNTKEAEAFALLSEEGIAKGIRRIVFVTQADAKKAIAAAAEFESKLEQAKQLDGEVLEAECKVLSQEVMGLTASAIKKSQFRDTISDLQKKVIAYKKEKIAGMTDEIVEKMVAAAGETS---GNKVIFRFDFGSDGKMVKNLTSAYGKQVQD-KAILILSADEDTDKLLVAAFAP--KGAKDVDCKAWLTAATQ--GTDGKGGGKKDMAQFHVPGVTNADSIL 957          

BLAST of mRNA_Ecto-sp13_S_contig6.16584.1 vs. uniprot
Match: A0A7S1ZG71_TRICV (Alanine--tRNA ligase n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1ZG71_TRICV)

HSP 1 Score: 996 bits (2576), Expect = 0.000e+0
Identity = 532/969 (54.90%), Postives = 650/969 (67.08%), Query Frame = 2

Query:  227 WPASRVRDSFISFFTEK-HDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRESDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAISSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDSKVFPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSXXXXXXXXXXXXXKAAG-GKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAKASFVLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDVGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDVVCKVDYKRRANVAPNHTMTHVLNFALRKVLDPSD----GMCDQKGSFLDDEKLRFDFSWSGALTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAKRIQSLRGVFGERYPDPVRVLSIGQD-VPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVMEERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTNKWVQSALDASGRGGKGGGKGDRAQGISQGVDTIDKAMEVVR 3112
            W    VR +F+ +F      HT   SSP VP  DPTLLFANAGMNQFKP+FLGT DP  P+S   RA NSQKCIRAGGKHNDLDDVGKD+YHHT FEMLGNWSFG YFKE AI  AW+CL   +KLD SRLYATYF GDE+     DE+A++IWLKYLP  RVLPF   DNFWEMG  GPCGPC EIHYDRIGGRDAA  VN D P+VIEIWN VFIQYNRE+DGSL+ LP +H+DTG+GFERL SILQGK SNYDTD+F+P+F  I   TGA+PY GKVG +D   +DMAYRV+ADHIRTL+ AI DG  PSNDGRGYVLRRVLRRA+RYG+Q LG   GFF  LVP +VGLM   FPEL   +  VTA+I+DEE SF+KTLDKGL  F ++A+ +GD KVF G  AF LY+SMGFP+DL  LMAEE GL++D EGFD KM                 G GK M L AEQ   L     EAT+   +Y W+Q+    A+   ALFVG+GE EDGVGFVPS +A     +   VG+VLD T+FYAE GGQIYD G +  S G G   V  V ++G + LHIG+V  GT+++G +++C VDYKRR+ +A NHTMTHVLNFAL+ VL           DQKGS +D+ KLRFDFSW+G LT  Q+  VED VND+I   +P+ ++VAPL++AK I SLR VFGE YPDPVRV+S+  + +P +LE+P++  W D S+EFCGGTHLSNT EA +FV+L+E GIAKG+RRI  VT   A AA ERA AF  +LD    ++  +++  ++ +  EL+G  IS   K   + +L  L +K  + KK+    R  +      A A  T+   G ++V R DFG D K A  +     K   +  + +L TAD +ADR+L     P       D   W  +A +  G G KGGGK D AQ  + GVD ID  +E  R
Sbjct:   86 WTTDAVRSTFVDYFASSPRSHTRVPSSPCVPLSDPTLLFANAGMNQFKPIFLGTVDPNTPMSDWKRAANSQKCIRAGGKHNDLDDVGKDVYHHTMFEMLGNWSFGDYFKEGAIDMAWQCLVEEFKLDASRLYATYFAGDEQ--TPPDEEARRIWLKYLPPDRVLPFDAKDNFWEMGATGPCGPCTEIHYDRIGGRDAASLVNADLPDVIEIWNNVFIQYNREADGSLRSLPAQHVDTGMGFERLTSILQGKDSNYDTDIFMPLFAEIEKITGAKPYGGKVGADDEGFVDMAYRVIADHIRTLSFAIADGAVPSNDGRGYVLRRVLRRAVRYGRQNLGADLGFFAQLVPTLVGLMGETFPELKKHQDHVTAIIRDEEESFSKTLDKGLVRFKEMASQVGDDKVFNGDDAFFLYASMGFPVDLTELMAEELGLTLDREGFDAKMKEEQDKSAEAHRKKMMGGSGKDMRLVAEQTARLVSDGVEATDDEAKYMWNQELGGCAA--KALFVGRGETEDGVGFVPSINA-----ESGMVGLVLDKTAFYAEAGGQIYDTGSIKTS-GGGVVRVDNVQAYGQFVLHIGEVTEGTVNLGDELICCVDYKRRSPIAANHTMTHVLNFALKDVLVTKGTDKIASVDQKGSLVDESKLRFDFSWAGPLTGAQLGAVEDAVNDRIRSAIPIQSYVAPLDKAKLISSLRAVFGEVYPDPVRVVSVSPEPIPEILENPQDGQWLDYSIEFCGGTHLSNTSEAGSFVLLKEEGIAKGIRRIFGVTGSDAQAATERADAFASKLDDTSKMDGDAMEGALKSMSEELNGLSISAARKEGFKDKLTELTKKVLSWKKEQAAARTKEVAQAVIAAASSTE---GDKVVIRNDFGIDGKVAKAVKGAFDKKVKD-KAFLLVTADVDADRFLVVASAPKGMKGV-DCKAWASAATE--GTGAKGGGKKDLAQFNATGVDKIDGVLEKAR 1037