mRNA_Ecto-sp13_S_contig5892.16409.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig5892.16409.1
Unique NamemRNA_Ecto-sp13_S_contig5892.16409.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig5892.16409.1 vs. uniprot
Match: D8LBA2_ECTSI (GPA4, alpha subunit of a heterotrimeric G protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LBA2_ECTSI)

HSP 1 Score: 220 bits (561), Expect = 2.550e-69
Identity = 106/108 (98.15%), Postives = 107/108 (99.07%), Query Frame = 1 
Query:    7 YWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFLAGLNGYNQRLFEDSSINKMRESLNLFRQV 330
            YWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFL GLNGY+QRLFEDSSINKMRESLNLFRQV
Sbjct:  145 YWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFLVGLNGYDQRLFEDSSINKMRESLNLFRQV 252
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BLAST of mRNA_Ecto-sp13_S_contig5892.16409.1 vs. uniprot
Match: A0A835YY90_9STRA (GPA4, alpha subunit of a heterotrimeric G protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YY90_9STRA)

HSP 1 Score: 170 bits (430), Expect = 1.910e-49
Identity = 76/108 (70.37%), Postives = 93/108 (86.11%), Query Frame = 1 
Query:    7 YWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFLAGLNGYNQRLFEDSSINKMRESLNLFRQV 330
            YWLLD A+YYFD   R+    F PT++DVIMTR+RTTGID+TDF EPP+ YSVVDVGGQRNERRKW+HCF+NV+ VV+L GL+GYNQ LFED+S+N+M+ESL LF+QV
Sbjct:  138 YWLLDAAAYYFDEVQRIADPGFEPTEDDVIMTRVRTTGIDSTDFVEPPFSYSVVDVGGQRNERRKWVHCFENVKAVVYLVGLSGYNQCLFEDASVNRMQESLKLFKQV 245
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BLAST of mRNA_Ecto-sp13_S_contig5892.16409.1 vs. uniprot
Match: A0A835ZKP0_9STRA (GPA4, alpha subunit of a heterotrimeric G protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZKP0_9STRA)

HSP 1 Score: 161 bits (408), Expect = 2.660e-46
Identity = 74/108 (68.52%), Postives = 91/108 (84.26%), Query Frame = 1 
Query:    7 YWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFLAGLNGYNQRLFEDSSINKMRESLNLFRQV 330
            YWLLD A+YYF+   R+    + PT+EDVIMTR+RTTGID T+F E PY +SVVDVGGQRNERRKWIHCF+NV+ VV+L GL+GYNQ LFED+S+N+M+ES+ LFRQV
Sbjct:  125 YWLLDAAAYYFNEVQRISDPKYEPTEEDVIMTRVRTTGIDHTEFIEHPYNWSVVDVGGQRNERRKWIHCFENVKAVVYLVGLSGYNQCLFEDASVNRMQESIKLFRQV 232
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BLAST of mRNA_Ecto-sp13_S_contig5892.16409.1 vs. uniprot
Match: A0A835Z6Q6_9STRA (GPA5, alpha subunit of a heterotrimeric G protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z6Q6_9STRA)

HSP 1 Score: 154 bits (390), Expect = 1.000e-43
Identity = 68/108 (62.96%), Postives = 91/108 (84.26%), Query Frame = 1 
Query:    7 YWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFLAGLNGYNQRLFEDSSINKMRESLNLFRQV 330
            +WLLD + YYF+ A+RL + D+ PTDED+IMTRIRTTGI  T+F + PY +S+VDVGGQR+ERRKWIHCFD+V+ ++FL GL+GY+Q LFED+++N+M ESL LF +V
Sbjct:  143 FWLLDASHYYFEEAMRLAEPDYEPTDEDMIMTRIRTTGIVVTEFEDKPYSFSLVDVGGQRSERRKWIHCFDDVKAIIFLEGLSGYHQVLFEDTAVNRMHESLQLFEEV 250
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BLAST of mRNA_Ecto-sp13_S_contig5892.16409.1 vs. uniprot
Match: A0A6S9GGW2_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6S9GGW2_HETAK)

HSP 1 Score: 149 bits (376), Expect = 1.220e-41
Identity = 70/109 (64.22%), Postives = 88/109 (80.73%), Query Frame = 1 
Query:    7 YWLLDGASYYFDNALRLGQADFV-PTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFLAGLNGYNQRLFEDSSINKMRESLNLFRQV 330
            YWLLD A YY D  LRLG AD+  PT+ED+IMTR+RTTGI  T+F + P  Y++VDVGGQR+ERRKWIHCFD+V+ ++FL GL+GYNQ LFED++ N+M ESL LF+ V
Sbjct:  144 YWLLDAAPYYLDEVLRLGAADYADPTEEDMIMTRVRTTGIVITEFDDGPSRYNIVDVGGQRSERRKWIHCFDDVKAIIFLVGLSGYNQVLFEDNTQNRMTESLELFQDV 252
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BLAST of mRNA_Ecto-sp13_S_contig5892.16409.1 vs. uniprot
Match: A0A6H5L0R0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L0R0_9PHAE)

HSP 1 Score: 144 bits (363), Expect = 1.310e-39
Identity = 63/110 (57.27%), Postives = 83/110 (75.45%), Query Frame = 1 
Query:    1 HRYWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFLAGLNGYNQRLFEDSSINKMRESLNLFRQV 330
            HRYWLLD A+YYFDN  R G   F P +ED++M R RT+GI+ T   +  + +SVVDVGGQR+ERRKWIHCFD+V+ ++F+  L+GYNQ LFED+ +N+M E L LF +V
Sbjct:  140 HRYWLLDAAAYYFDNVERFGSESFAPNEEDMLMARARTSGINVTQIADGLHKFSVVDVGGQRSERRKWIHCFDDVKAIIFVVALSGYNQVLFEDTGVNRMHEGLQLFEEV 249
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BLAST of mRNA_Ecto-sp13_S_contig5892.16409.1 vs. uniprot
Match: A0A8J4Q695_9MYCE (Uncharacterized protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4Q695_9MYCE)

HSP 1 Score: 141 bits (356), Expect = 1.710e-38
Identity = 66/110 (60.00%), Postives = 82/110 (74.55%), Query Frame = 1 
Query:    7 YWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFLAGLNGYNQRLFEDSSINKMRESLNLFRQVNN 336
            YW LD   YYFDN  R    DF+PT+ED IMTR+RTTGI  T+F E P  + VVDVGGQRNER+KWIHCFD+V+ ++F+  L GY+Q +FED S N+M+ESL LF Q+ N
Sbjct:  146 YWFLDATPYYFDNIQRFLDDDFIPTEEDCIMTRVRTTGISVTEFDEGPVHFRVVDVGGQRNERKKWIHCFDDVKALLFVVNLAGYDQVMFEDPSQNRMQESLTLFGQICN 255
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BLAST of mRNA_Ecto-sp13_S_contig5892.16409.1 vs. uniprot
Match: D8LTN0_ECTSI (GPA5, alpha subunit of a heterotrimeric G protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LTN0_ECTSI)

HSP 1 Score: 141 bits (356), Expect = 1.920e-38
Identity = 65/108 (60.19%), Postives = 84/108 (77.78%), Query Frame = 1 
Query:    7 YWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFLAGLNGYNQRLFEDSSINKMRESLNLFRQV 330
            +W LD   YY   A RL   +   TDED+IMTR RTTGI  T+F++ PY YS+VDVGGQR+ERRKW+HCFD+V+ +VFL GL+GY+Q LFED+S+N+M+ESL LF +V
Sbjct:  146 FWALDATPYYLQEARRLADPELEVTDEDMIMTRTRTTGIIVTEFSDNPYTYSLVDVGGQRSERRKWMHCFDDVKAIVFLVGLSGYHQVLFEDNSVNRMQESLELFTEV 253
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BLAST of mRNA_Ecto-sp13_S_contig5892.16409.1 vs. uniprot
Match: A0A1Y2AMP6_9FUNG (Guanine nucleotide binding protein, alpha subunit n=1 Tax=Neocallimastix californiae TaxID=1754190 RepID=A0A1Y2AMP6_9FUNG)

HSP 1 Score: 140 bits (352), Expect = 5.170e-38
Identity = 61/110 (55.45%), Postives = 83/110 (75.45%), Query Frame = 1 
Query:    7 YWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFLAGLNGYNQRLFEDSSINKMRESLNLFRQVNN 336
            +W++D A YYFDN +R    DF  T+ED +M+RI TTGI +T+   PP  ++V+DVGGQRNERRKW+HCFDNV  ++F+  LNGYN  L+ED+S+N+M+E  NLF+Q  N
Sbjct:  136 FWIMDAAYYYFDNVMRFVDEDFELTEEDYVMSRIMTTGIISTEINVPPLKFTVIDVGGQRNERRKWLHCFDNVSALLFIVNLNGYNSVLYEDNSVNRMQECFNLFKQTAN 245
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BLAST of mRNA_Ecto-sp13_S_contig5892.16409.1 vs. uniprot
Match: A0A1Y1VF76_9FUNG (G-protein subunit alpha 8 n=1 Tax=Piromyces finnis TaxID=1754191 RepID=A0A1Y1VF76_9FUNG)

HSP 1 Score: 140 bits (352), Expect = 6.930e-38
Identity = 62/110 (56.36%), Postives = 83/110 (75.45%), Query Frame = 1 
Query:    7 YWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPPYMYSVVDVGGQRNERRKWIHCFDNVRCVVFLAGLNGYNQRLFEDSSINKMRESLNLFRQVNN 336
            +W++D A YYFDN LR    DF  T+ED +M+RI TTGI +T+   PP  ++V+DVGGQRNERRKW+HCFDNV  ++++  LNGYN  LFED+S+N+M+E  NLF+Q  N
Sbjct:  150 FWIMDAAYYYFDNVLRFIDDDFELTEEDYVMSRIMTTGIISTEINVPPLKFTVIDVGGQRNERRKWLHCFDNVSALLYIVNLNGYNSVLFEDNSVNRMQECFNLFKQTAN 259
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig5892.16409.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LBA2_ECTSI2.550e-6998.15GPA4, alpha subunit of a heterotrimeric G protein ... [more]
A0A835YY90_9STRA1.910e-4970.37GPA4, alpha subunit of a heterotrimeric G protein ... [more]
A0A835ZKP0_9STRA2.660e-4668.52GPA4, alpha subunit of a heterotrimeric G protein ... [more]
A0A835Z6Q6_9STRA1.000e-4362.96GPA5, alpha subunit of a heterotrimeric G protein ... [more]
A0A6S9GGW2_HETAK1.220e-4164.22Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
A0A6H5L0R0_9PHAE1.310e-3957.27Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A8J4Q695_9MYCE1.710e-3860.00Uncharacterized protein n=1 Tax=Polysphondylium vi... [more]
D8LTN0_ECTSI1.920e-3860.19GPA5, alpha subunit of a heterotrimeric G protein ... [more]
A0A1Y2AMP6_9FUNG5.170e-3855.45Guanine nucleotide binding protein, alpha subunit ... [more]
A0A1Y1VF76_9FUNG6.930e-3856.36G-protein subunit alpha 8 n=1 Tax=Piromyces finnis... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig5892contigEcto-sp13_S_contig5892:7118..7883 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score224.2
Seed ortholog evalue3.1e-56
Seed eggNOG ortholog2880.D8LBA2
Preferred namegpaH
Model size336
KEGG koko:K04630
KEGG Pathwayko04015,ko04022,ko04024,ko04062,ko04071,ko04261,ko04360,ko04371,ko04540,ko04611,ko04670,ko04713,ko04723,ko04724,ko04725,ko04726,ko04727,ko04728,ko04730,ko04914,ko04915,ko04916,ko04921,ko04923,ko04924,ko04926,ko04934,ko04971,ko05012,ko05030,ko05032,ko05034,ko05133,ko05142,ko05145,ko05200,map04015,map04022,map04024,map04062,map04071,map04261,map04360,map04371,map04540,map04611,map04670,map04713,map04723,map04724,map04725,map04726,map04727,map04728,map04730,map04914,map04915,map04916,map04921,map04923,map04924,map04926,map04934,map04971,map05012,map05030,map05032,map05034,map05133,map05142,map05145,map05200
KEGG ModuleM00695
Hectar predicted targeting categoryother localisation
GOsGO:0001664,GO:0003674,GO:0003824,GO:0003924,GO:0005102,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005834,GO:0005886,GO:0007154,GO:0007165,GO:0007186,GO:0007187,GO:0007188,GO:0008150,GO:0009898,GO:0009966,GO:0009987,GO:0010469,GO:0010646,GO:0010810,GO:0010811,GO:0016020,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0017111,GO:0019897,GO:0019898,GO:0022407,GO:0022409,GO:0023051,GO:0023052,GO:0030155,GO:0031234,GO:0031683,GO:0032092,GO:0032101,GO:0032103,GO:0032991,GO:0040012,GO:0040017,GO:0043393,GO:0043937,GO:0043938,GO:0044093,GO:0044424,GO:0044425,GO:0044459,GO:0044464,GO:0044877,GO:0045595,GO:0045785,GO:0048518,GO:0048519,GO:0048583,GO:0048584,GO:0050789,GO:0050793,GO:0050794,GO:0050896,GO:0050920,GO:0050921,GO:0050923,GO:0050924,GO:0051094,GO:0051098,GO:0051099,GO:0051716,GO:0065007,GO:0065009,GO:0071944,GO:0098552,GO:0098562,GO:0098796,GO:0098797,GO:1900120,GO:1900122,GO:1902494,GO:1903664,GO:1903665,GO:1903667,GO:1903669,GO:1905360
Exons2
EggNOG free text desc.G-protein beta/gamma-subunit complex binding
EggNOG OGsKOG0082@1,KOG0082@2759
Cds size336
COG Functional cat.V
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko04031,ko04147
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463818.9606395-CDS-Ecto-sp13_S_contig5892:7117..73451681463818.9606395-CDS-Ecto-sp13_S_contig5892:7117..7345Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig5892 7118..7345 +
1681463818.9738865-CDS-Ecto-sp13_S_contig5892:7775..78831681463818.9738865-CDS-Ecto-sp13_S_contig5892:7775..7883Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig5892 7776..7883 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig5892.16409.1prot_Ecto-sp13_S_contig5892.16409.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig5892 7118..7883 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig5892.16409.1

>prot_Ecto-sp13_S_contig5892.16409.1 ID=prot_Ecto-sp13_S_contig5892.16409.1|Name=mRNA_Ecto-sp13_S_contig5892.16409.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=112bp
HRYWLLDGASYYFDNALRLGQADFVPTDEDVIMTRIRTTGIDTTDFTEPP
YMYSVVDVGGQRNERRKWIHCFDNVRCVVFLAGLNGYNQRLFEDSSINKM
RESLNLFRQVNN
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mRNA from alignment at Ecto-sp13_S_contig5892:7118..7883+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig5892.16409.1 ID=mRNA_Ecto-sp13_S_contig5892.16409.1|Name=mRNA_Ecto-sp13_S_contig5892.16409.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=766bp|location=Sequence derived from alignment at Ecto-sp13_S_contig5892:7118..7883+ (Ectocarpus species13 EcNAP12_S_4_19m)
CACAGGTACTGGCTGCTTGACGGGGCGAGCTACTACTTCGACAACGCCCT
GAGATTGGGCCAAGCGGACTTCGTCCCCACAGACGAAGACGTGATCATGA
CGCGCATCAGGACCACGGGAATCGACACCACGGATTTCACAGAGCCCCCG
TACATGTACTCGGTGGTGGACGTGGGGGGCCAGAGGAACGAGAGGAGAAA
GTGGATCCACTGCTTCGACAACGTTCGGGTAGGAATTATGATATATCGTG
CGGCGAGGGGGGCAGGCGGGTCTAGACTAATCGTGGATGGAGTTTTTGTT
TTTCGTCTTGCTTCTTCCTTGAGGCTGGTTGTGCTTTCGTGGAAGAACGG
GTGTGTGCCGGATTTTACGATTCTGGGTTGTTTCGCTCTTTTTCCCTCTC
GTCCGTTTTATCTCTTCCTCCCTCCCTCCCTCCCTCCCTCCCTTTCGCTC
TCCCTCGTTCCTCCCCTACCTGTTCATGGGTTCGCCTTCAAAGAACAGCA
TCGATAGCGCGAACCGACTGACCAATCAGAACACGCCAGACTCAGTGCGG
CTAACGCTGTGTGTGTGCGCGCGCCTCTTCCTCTTGTCCTGCCGGCCTCT
AACCGCGCCTTCCCTCCTGCTGCTGCTACAACGATACGAATTGATAAAAA
AAATACAGTGTGTGGTGTTCCTGGCAGGCTTGAACGGCTACAACCAGCGT
CTGTTTGAGGACAGCAGCATCAACAAGATGCGGGAAAGCCTCAACCTCTT
CCGGCAGGTGAATAAT
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig5892:7118..7883+

>mRNA_Ecto-sp13_S_contig5892.16409.1 ID=mRNA_Ecto-sp13_S_contig5892.16409.1|Name=mRNA_Ecto-sp13_S_contig5892.16409.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=336bp|location=Sequence derived from alignment at Ecto-sp13_S_contig5892:7118..7883+ (Ectocarpus species13 EcNAP12_S_4_19m)
CACAGGTACTGGCTGCTTGACGGGGCGAGCTACTACTTCGACAACGCCCT
GAGATTGGGCCAAGCGGACTTCGTCCCCACAGACGAAGACGTGATCATGA
CGCGCATCAGGACCACGGGAATCGACACCACGGATTTCACAGAGCCCCCG
TACATGTACTCGGTGGTGGACGTGGGGGGCCAGAGGAACGAGAGGAGAAA
GTGGATCCACTGCTTCGACAACGTTCGGTGTGTGGTGTTCCTGGCAGGCT
TGAACGGCTACAACCAGCGTCTGTTTGAGGACAGCAGCATCAACAAGATG
CGGGAAAGCCTCAACCTCTTCCGGCAGGTGAATAAT
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