mRNA_Ecto-sp13_S_contig11565.1362.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig11565.1362.1
Unique NamemRNA_Ecto-sp13_S_contig11565.1362.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig11565.1362.1 vs. uniprot
Match: A0A6H5JEW3_9PHAE (S-formylglutathione hydrolase n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JEW3_9PHAE)

HSP 1 Score: 214 bits (544), Expect = 6.100e-62
Identity = 113/141 (80.14%), Postives = 122/141 (86.52%), Query Frame = 1 
Query:    4 NMKRSSSPLRGADSIMASSSSGAATDLEIKSETACFGGRLLKCVHQSKATKTPMTFTVFLPPSAASSAVPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGEVCSTPVGVGPAFRRAVAIARRSVY 426
            N+KR   PLRGADSIMAS S GAA DLEIKSETACFGGRLLKCVHQSKATKTPMTFTVFLPP+AASSAVPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGE  ++  G+G  + R     +++ Y
Sbjct:   46 NIKRXXXPLRGADSIMASXSGGAAADLEIKSETACFGGRLLKCVHQSKATKTPMTFTVFLPPAAASSAVPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGEAGTSAQGLGSTWTRRKRTGKKTRY 186
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BLAST of mRNA_Ecto-sp13_S_contig11565.1362.1 vs. uniprot
Match: A0A7S2UZ85_9STRA (S-formylglutathione hydrolase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UZ85_9STRA)

HSP 1 Score: 130 bits (327), Expect = 4.520e-31
Identity = 64/103 (62.14%), Postives = 75/103 (72.82%), Query Frame = 1 
Query:   82 LEIKSETACFGGRLLKCVHQSKATKTPMTFTVFLPPSAASSAVPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGEVCSTPVGVGPAF 390
            LE+K E  CFGG L +  HQS+A  TPM F++FLPP A SS VPVLYYLSGLTCTD+NF QK GA R+A+  G+A+VAPDTSPRGAG+ GE      G G  F
Sbjct:    5 LEVKKEWKCFGGTLKQLTHQSEAVGTPMRFSIFLPPQAESSPVPVLYYLSGLTCTDENFTQKGGAYRLASKLGIAVVAPDTSPRGAGIEGEDSGWDFGTGAGF 107
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BLAST of mRNA_Ecto-sp13_S_contig11565.1362.1 vs. uniprot
Match: A0A7S1SFB8_ALECA (S-formylglutathione hydrolase (Fragment) n=1 Tax=Alexandrium catenella TaxID=2925 RepID=A0A7S1SFB8_ALECA)

HSP 1 Score: 120 bits (300), Expect = 4.780e-28
Identity = 67/108 (62.04%), Postives = 73/108 (67.59%), Query Frame = 1 
Query:   73 ATDLEIKSETACFGGRLLKCVHQSKATKTPMTFTVFLPPSAASSA--VPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGEVCSTPVGVGPAF 390
            A  LE K E    GGR+ K  H S+AT TPMTF+VFLPP  A +   VPVLYYLSGLTCTDDN IQKA AQR AA  G+  VAPDTSPRGAG+ GE  S   G G  F
Sbjct:   20 AAGLEKKGENRMCGGRVQKWAHASEATGTPMTFSVFLPPQVAQAGTKVPVLYYLSGLTCTDDNVIQKACAQRAAAAHGMVFVAPDTSPRGAGIEGEDDSYDFGSGAGF 127
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BLAST of mRNA_Ecto-sp13_S_contig11565.1362.1 vs. uniprot
Match: UPI0013E827B6 (S-formylglutathione hydrolase-like n=1 Tax=Panicum virgatum TaxID=38727 RepID=UPI0013E827B6)

HSP 1 Score: 119 bits (299), Expect = 4.260e-27
Identity = 65/105 (61.90%), Postives = 75/105 (71.43%), Query Frame = 1 
Query:   76 TDLEIKSETACFGGRLLKCVHQSKATKTPMTFTVFLPPSAASSAVPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGEVCSTPVGVGPAF 390
            T++E+ S    FGG  L+  HQS     PMTFTV+LPPS AS+ VPVLY+LSGLTCTD+NFI KAGAQR AA  G+ALVAPDTSPRG  + GE  S   GVG  F
Sbjct:    8 TEVELLSTNKMFGGHNLRYRHQSATLGCPMTFTVYLPPSPASN-VPVLYWLSGLTCTDENFIIKAGAQRAAAAHGIALVAPDTSPRGLNIEGESDSYDFGVGAGF 111
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BLAST of mRNA_Ecto-sp13_S_contig11565.1362.1 vs. uniprot
Match: A0A6V1VPS3_HETAK (S-formylglutathione hydrolase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1VPS3_HETAK)

HSP 1 Score: 119 bits (299), Expect = 5.040e-27
Identity = 62/103 (60.19%), Postives = 71/103 (68.93%), Query Frame = 1 
Query:   82 LEIKSETACFGGRLLKCVHQSKATKTPMTFTVFLPPSAASSAVPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGEVCSTPVGVGPAF 390
            LE+KS   C GG + +  HQS +T TPM FT+FLPP +  S VPVLYYLSGLTCTD+N +QK GA R AA  G+ALV PDTSPRGAGV GE      G G  F
Sbjct:   11 LELKSSWKCHGGFVRQYSHQSSSTGTPMRFTIFLPPESCGSDVPVLYYLSGLTCTDENVVQKGGAFRAAARAGLALVCPDTSPRGAGVEGEDEGWDFGTGAGF 113
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BLAST of mRNA_Ecto-sp13_S_contig11565.1362.1 vs. uniprot
Match: A0A7S4V882_9DINO (S-formylglutathione hydrolase (Fragment) n=1 Tax=Alexandrium monilatum TaxID=311494 RepID=A0A7S4V882_9DINO)

HSP 1 Score: 120 bits (300), Expect = 5.490e-27
Identity = 66/110 (60.00%), Postives = 76/110 (69.09%), Query Frame = 1 
Query:   67 GAATDLEIKSETACFGGRLLKCVHQSKATKTPMTFTVFLPPSA--ASSAVPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGEVCSTPVGVGPAF 390
            G A+ LE K E   FGG++ K  H S++T TPMTF+VFLPP    A + VPVLYYLSGLTCTDDN  QKA AQR AA  G+ +VAPDTSPRGAG+ GE  S   G G  F
Sbjct:   27 GFASGLEKKGENRMFGGQVQKWTHASESTGTPMTFSVFLPPQVTQAGTKVPVLYYLSGLTCTDDNVTQKACAQRAAAAHGMVIVAPDTSPRGAGIEGEDESYDFGSGAGF 136
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BLAST of mRNA_Ecto-sp13_S_contig11565.1362.1 vs. uniprot
Match: A0A7S2STF8_9STRA (S-formylglutathione hydrolase (Fragment) n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2STF8_9STRA)

HSP 1 Score: 119 bits (298), Expect = 1.290e-26
Identity = 66/122 (54.10%), Postives = 80/122 (65.57%), Query Frame = 1 
Query:   25 PLRGADSIMASSSSGAATDLEIKSETACFGGRLLKCVHQSKATKTPMTFTVFLPPSAASSAVPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGEVCSTPVGVGPAF 390
            P R A    +S+S GAA    +      FGG   +  H+S AT TPMTF+VFLPP AA+  VP ++YLSGLTCTD+NF+QKAGA R AA   +A+VAPDTSPRGAGV GE  +   G G  F
Sbjct:   22 PWRSALRSCSSTSGGAAAPPRVVGSHKSFGGVWTRYEHESAATGTPMTFSVFLPPRAAAGPVPCVFYLSGLTCTDENFVQKAGAARAAAALQLAIVAPDTSPRGAGVQGEEDAYDFGTGAGF 143
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BLAST of mRNA_Ecto-sp13_S_contig11565.1362.1 vs. uniprot
Match: B7F3S0_ORYSJ (S-formylglutathione hydrolase n=5 Tax=Oryza TaxID=4527 RepID=B7F3S0_ORYSJ)

HSP 1 Score: 113 bits (282), Expect = 1.630e-26
Identity = 63/110 (57.27%), Postives = 74/110 (67.27%), Query Frame = 1 
Query:   82 LEIKSETACFGGRLLKCVHQSKATKTPMTFTVFLPPSAASSAVPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGEVCSTPVGVGPAFRRAVAIA 411
            LE  S T  FGG  L+  H S     PMTF+VFLPPS AS  +PVLY+LSGLTC D+NF+ KAGAQR AA  G+ALVAPDTSPRG  + GE  S   GVG +  + V I+
Sbjct:   14 LEQMSRTKMFGGHNLRFRHHSATLGCPMTFSVFLPPSPASD-LPVLYWLSGLTCNDENFVTKAGAQRAAAAHGIALVAPDTSPRGLNIEGEADSWDFGVGLSTLKTVNIS 122
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BLAST of mRNA_Ecto-sp13_S_contig11565.1362.1 vs. uniprot
Match: UPI001AA08FDA (S-formylglutathione hydrolase n=1 Tax=Aestuariicella hydrocarbonica TaxID=1470433 RepID=UPI001AA08FDA)

HSP 1 Score: 117 bits (294), Expect = 1.720e-26
Identity = 58/91 (63.74%), Postives = 71/91 (78.02%), Query Frame = 1 
Query:   82 LEIKSETACFGGRLLKCVHQSKATKTPMTFTVFLPPSAASSAVPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGE 354
            +EI +E  CFGGR L+  H+S A    M  ++F+PP A +S VPVLY+LSGLTCTD+NF+QKAGAQRIAA  GVA+VAPDTSPRG GVA +
Sbjct:    1 MEIVAENKCFGGRQLQFTHESSALNCTMRCSIFVPPQADTSKVPVLYWLSGLTCTDENFVQKAGAQRIAAELGVAIVAPDTSPRGEGVADD 91
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BLAST of mRNA_Ecto-sp13_S_contig11565.1362.1 vs. uniprot
Match: F0Y275_AURAN (S-formylglutathione hydrolase n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y275_AURAN)

HSP 1 Score: 117 bits (293), Expect = 2.950e-26
Identity = 61/97 (62.89%), Postives = 71/97 (73.20%), Query Frame = 1 
Query:  103 ACFGGRLLKCVHQSKATKTPMTFTVFLPPSAASSA-VPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVALVAPDTSPRGAGVAGEVCSTPVGVGPAF 390
            ACFGG   +  H S+ TKTPM F+VF+PP+A   A  P ++YLSGLTCTD+NF+QKAGA R AA RGVALVAPDTSPRGAGV GE  +   G G  F
Sbjct:   11 ACFGGSWQRYKHDSECTKTPMAFSVFVPPAATPEAPAPCIFYLSGLTCTDENFVQKAGAARAAAERGVALVAPDTSPRGAGVDGEDDAYDFGSGAGF 107
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig11565.1362.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JEW3_9PHAE6.100e-6280.14S-formylglutathione hydrolase n=2 Tax=Ectocarpus T... [more]
A0A7S2UZ85_9STRA4.520e-3162.14S-formylglutathione hydrolase n=1 Tax=Fibrocapsa j... [more]
A0A7S1SFB8_ALECA4.780e-2862.04S-formylglutathione hydrolase (Fragment) n=1 Tax=A... [more]
UPI0013E827B64.260e-2761.90S-formylglutathione hydrolase-like n=1 Tax=Panicum... [more]
A0A6V1VPS3_HETAK5.040e-2760.19S-formylglutathione hydrolase n=1 Tax=Heterosigma ... [more]
A0A7S4V882_9DINO5.490e-2760.00S-formylglutathione hydrolase (Fragment) n=1 Tax=A... [more]
A0A7S2STF8_9STRA1.290e-2654.10S-formylglutathione hydrolase (Fragment) n=2 Tax=R... [more]
B7F3S0_ORYSJ1.630e-2657.27S-formylglutathione hydrolase n=5 Tax=Oryza TaxID=... [more]
UPI001AA08FDA1.720e-2663.74S-formylglutathione hydrolase n=1 Tax=Aestuariicel... [more]
F0Y275_AURAN2.950e-2662.89S-formylglutathione hydrolase n=1 Tax=Aureococcus ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig11565contigEcto-sp13_S_contig11565:360..2523 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score192.6
Seed ortholog evalue1.7e-46
Seed eggNOG ortholog2880.D7FI83
Model size1341
KEGG rclassRC00167,RC00320
KEGG koko:K01070
KEGG ReactionR00527
KEGG Pathwayko00680,ko01120,ko01200,map00680,map01120,map01200
Hectar predicted targeting categoryother localisation
Exons2
EggNOG free text desc.S-formylglutathione hydrolase activity
EggNOG OGsCOG0627@1,KOG3101@2759
EC3.1.2.12
Cds size570
COG Functional cat.C
CAZyCE1
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462607.304137-UTR-Ecto-sp13_S_contig11565:359..3651681462607.304137-UTR-Ecto-sp13_S_contig11565:359..365Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig11565 360..365 +
1681462607.3381665-UTR-Ecto-sp13_S_contig11565:1758..25231681462607.3381665-UTR-Ecto-sp13_S_contig11565:1758..2523Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig11565 1759..2523 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462607.3167615-CDS-Ecto-sp13_S_contig11565:365..5721681462607.3167615-CDS-Ecto-sp13_S_contig11565:365..572Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11565 366..572 +
1681462607.326225-CDS-Ecto-sp13_S_contig11565:1395..17581681462607.326225-CDS-Ecto-sp13_S_contig11565:1395..1758Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11565 1396..1758 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig11565.1362.1prot_Ecto-sp13_S_contig11565.1362.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig11565 366..1758 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig11565.1362.1

>prot_Ecto-sp13_S_contig11565.1362.1 ID=prot_Ecto-sp13_S_contig11565.1362.1|Name=mRNA_Ecto-sp13_S_contig11565.1362.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=190bp
MKRSSSPLRGADSIMASSSSGAATDLEIKSETACFGGRLLKCVHQSKATK
TPMTFTVFLPPSAASSAVPVLYYLSGLTCTDDNFIQKAGAQRIAALRGVA
LVAPDTSPRGAGVAGEVCSTPVGVGPAFRRAVAIARRSVYVTCDFLRLES
TRRRQIDVGWNRNLEIFLAFGCPPRQSVTALVCFLCFGP*
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mRNA from alignment at Ecto-sp13_S_contig11565:360..2523+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig11565.1362.1 ID=mRNA_Ecto-sp13_S_contig11565.1362.1|Name=mRNA_Ecto-sp13_S_contig11565.1362.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2164bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11565:360..2523+ (Ectocarpus species13 EcNAP12_S_4_19m)
GACAACATGAAGCGATCATCCTCGCCCTTGCGAGGAGCAGACAGCATCAT
GGCATCATCATCAAGTGGTGCCGCCACCGACCTTGAGATCAAGAGTGAGA
CCGCGTGCTTCGGGGGTAGGTTGCTCAAGTGCGTGCACCAGTCGAAGGCG
ACCAAGACTCCGATGACCTTCACCGTGTTTCTCCCCCCCTCGGCGGCATC
CAGCGCTGTGCCGGTCTGTATGTATATCTACTGCTGTAAAAGTATTATAC
ATGTGGTCTACAACAAAGATCTTGGCACAATCGATGTTTGCTACTGCTGC
TGTTGCGGACCTGTACTGTGTGCTGTCGGCGTCTCAGACGCTGCTGCGGA
CCGCTAAATGTACCCGATGTGGACTCTGACGACCTGCTACAGCAGTACAT
CGGTAGTACCCGTAGTACAGAGTCTAGGTGGTCATGTGCGGAAGAGAGGA
AATGCCTCCCTGTCGTCCCTTTCGCATCGTTCTAAATATGGAACAAGACG
TCGTTTCACTAGAATGCGTCACGCTCTCCCATGCCGATACACGTGCACAT
GTAGAGCTCGGCTGATGATCACTGATAAGAAGGTAGGTTTGCGGCACCTC
ATTCCCCTGGATACAGAAACGTGACTTGCTCGTAGACCGCCTCCCAGGTG
CCTCTTCGGACGATAGGCTCATTCATCCACAAGGTCGGCACCTGTACTAC
TGCAGACAGACGCGCGCTGTTGTTCTACTCGCCTCTGTCAAAAACTCTAG
TTCGTCGTTATCCCTGCCGGCCCAAATTTTTTTTTTCTTTTACCCACCGT
TTCGTCGCTAGTTTTTCTTGGCCCCAACGACGAGACCTTGCAATTTTCCC
GGCCCTTTCCACGTATTTTGTTCACACCAACGGGTGAGTGATAGGGTGTT
TGACCCGTCACTACGGAACCGGCCGTTTTTACCTTAATCGACGGGTATTG
TACGAATACCGCATGCGCAACACGTTGTGGAGGCCAGAATGTTCCTTTTG
TGAGAATACAACGTCAACGCCAACAACGATGCGCAGGTGCTCTACTACCT
TAGCGGTCTTACGTGCACCGACGACAACTTCATCCAAAAGGCTGGGGCAC
AGCGGATCGCAGCACTCAGGGGGGTAGCACTCGTCGCCCCGGACACGAGC
CCGCGGGGTGCCGGAGTTGCTGGAGAGGTGTGCTCAACTCCAGTCGGCGT
GGGCCCTGCTTTCCGCCGAGCGGTAGCAATAGCTCGAAGGAGTGTGTACG
TGACGTGTGATTTCCTTCGCTTAGAATCTACGCGCAGAAGGCAAATTGAT
GTCGGCTGGAACCGAAATTTAGAAATCTTTCTAGCCTTCGGCTGTCCCCC
CCGTCAAAGTGTGACGGCCCTGGTGTGTTTCCTGTGTTTCGGCCCGTGAC
TGAAAAACCAGGCCGGGGAGTCTCGACGTTACCGGAGAGGTACGTTCGGC
GGCGCTTCGGGTAAAGTCTATCTTCTAAGTGCATAACCTCGTCTGATAGC
TGCAGGTCCGCAGCAGGATCTCCTTGGGTGGTTGACTTCTTGCGCATACT
GGTGTTGCTGAGGCGAGTACGTTTGAGGCTTTTCCCGGCGTCGCCCGACG
GTGGGTGATATTGTGGTAGAGTTGTCTCTGCTGATTGTCGTTCTTAGATC
CAAACGAGGCTTTAATAAAGGTTCCAACACCCAGCGGATCGCGAGGCGGG
AAACGGCACATCACGAAATTAGCCTGAGCGCGCGTGCACGTTGCGTGTTG
TTGCGGTCACACGTATATATATGGCATGGTGCAAACGCGGAGCGCGAAAT
ACTTCGTTCGGCCGGCTGTTCGTGCTCTCTCGCTTGCAGGACGAAAACTG
GGACTTCGGCACGGGGGCTGGGTTCTACGTGGACGCGACGGAAGCGGGCT
GGAAAGAAAACGCGAGTGGCACGAGGATTTTCCCCGGGTCTGCTGTCTCG
TAGAGTAGACGATCTCTGGGGAAGTCTGGACGGATAGGATATTTGGAGTA
CACACTGAACGGCAGAAGATTGAAGAGACGCATGGTTGTTTGTCGCCCCT
GGCATCGTTGGTGTTGGTTGTGCTCCGTTAGCCGCCCCGCCAAAAATCAT
GGCGCCTCGTCCTGCTGGTGTAACTCCACGAAGACTGCTCTACGTGATGA
AGCCACCCCCACCC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig11565:360..2523+

>mRNA_Ecto-sp13_S_contig11565.1362.1 ID=mRNA_Ecto-sp13_S_contig11565.1362.1|Name=mRNA_Ecto-sp13_S_contig11565.1362.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=570bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11565:360..2523+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGAAGCGATCATCCTCGCCCTTGCGAGGAGCAGACAGCATCATGGCATC
ATCATCAAGTGGTGCCGCCACCGACCTTGAGATCAAGAGTGAGACCGCGT
GCTTCGGGGGTAGGTTGCTCAAGTGCGTGCACCAGTCGAAGGCGACCAAG
ACTCCGATGACCTTCACCGTGTTTCTCCCCCCCTCGGCGGCATCCAGCGC
TGTGCCGGTGCTCTACTACCTTAGCGGTCTTACGTGCACCGACGACAACT
TCATCCAAAAGGCTGGGGCACAGCGGATCGCAGCACTCAGGGGGGTAGCA
CTCGTCGCCCCGGACACGAGCCCGCGGGGTGCCGGAGTTGCTGGAGAGGT
GTGCTCAACTCCAGTCGGCGTGGGCCCTGCTTTCCGCCGAGCGGTAGCAA
TAGCTCGAAGGAGTGTGTACGTGACGTGTGATTTCCTTCGCTTAGAATCT
ACGCGCAGAAGGCAAATTGATGTCGGCTGGAACCGAAATTTAGAAATCTT
TCTAGCCTTCGGCTGTCCCCCCCGTCAAAGTGTGACGGCCCTGGTGTGTT
TCCTGTGTTTCGGCCCGTGA
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