mRNA_Ecto-sp13_S_contig19524.6285.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig19524.6285.1
Unique NamemRNA_Ecto-sp13_S_contig19524.6285.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig19524.6285.1 vs. uniprot
Match: D7FW88_ECTSI (40S ribosomal protein S21 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FW88_ECTSI)

HSP 1 Score: 133 bits (334), Expect = 3.610e-36
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 1 
Query:    1 SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKDESEL 192
            SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKDESEL
Sbjct:   18 SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKDESEL 81
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BLAST of mRNA_Ecto-sp13_S_contig19524.6285.1 vs. uniprot
Match: A0A1Z5KP36_FISSO (40S ribosomal protein S21 n=1 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5KP36_FISSO)

HSP 1 Score: 81.6 bits (200), Expect = 2.790e-16
Identity = 37/64 (57.81%), Postives = 49/64 (76.56%), Query Frame = 1 
Query:    1 SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKDESEL 192
            S+TNRL+QAKDH++ QINV  +DPV+G  TG+S+VY LAGY+R + E D ALT LV  KD + +
Sbjct:   18 SYTNRLIQAKDHAAVQINVANVDPVTGIMTGDSNVYCLAGYIRFKSEGDMALTSLVRAKDAAAV 81
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BLAST of mRNA_Ecto-sp13_S_contig19524.6285.1 vs. uniprot
Match: G4ZUD9_PHYSP (40S ribosomal protein S21 n=7 Tax=Peronosporaceae TaxID=4777 RepID=G4ZUD9_PHYSP)

HSP 1 Score: 80.1 bits (196), Expect = 9.280e-16
Identity = 37/60 (61.67%), Postives = 47/60 (78.33%), Query Frame = 1 
Query:    1 SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKD 180
            SWTNR+L AKDH+S QINVG+++  +G FTGESD YALAGY+R  GE D+A+T L  + D
Sbjct:   18 SWTNRILAAKDHASVQINVGRVN-ANGVFTGESDTYALAGYIRHHGEGDQAITELARQTD 76
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BLAST of mRNA_Ecto-sp13_S_contig19524.6285.1 vs. uniprot
Match: K3WSU9_GLOUD (40S ribosomal protein S21 n=2 Tax=Pythiaceae TaxID=4782 RepID=K3WSU9_GLOUD)

HSP 1 Score: 78.2 bits (191), Expect = 4.920e-15
Identity = 37/60 (61.67%), Postives = 47/60 (78.33%), Query Frame = 1 
Query:    1 SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKD 180
            SWTNR+L AKDH+S QINV +++  +G FTGESD +ALAGY+R +GEAD ALT L  + D
Sbjct:   18 SWTNRILAAKDHASVQINVARVN-ANGVFTGESDTFALAGYIRSKGEADLALTELARQAD 76
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BLAST of mRNA_Ecto-sp13_S_contig19524.6285.1 vs. uniprot
Match: A0A448Z9N0_9STRA (40S ribosomal protein S21 n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448Z9N0_9STRA)

HSP 1 Score: 76.6 bits (187), Expect = 2.190e-14
Identity = 34/60 (56.67%), Postives = 43/60 (71.67%), Query Frame = 1 
Query:    1 SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKD 180
            +WTNRL++AKDHSS Q+NV  +DPV+G  TG++  Y L GY+R   E D ALT LV K D
Sbjct:   18 NWTNRLVEAKDHSSVQVNVANVDPVTGLATGDATPYCLVGYIRKISEGDMALTALVEKND 77
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BLAST of mRNA_Ecto-sp13_S_contig19524.6285.1 vs. uniprot
Match: A0A7S1UVR1_9STRA (40S ribosomal protein S21 n=1 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1UVR1_9STRA)

HSP 1 Score: 76.3 bits (186), Expect = 3.220e-14
Identity = 36/60 (60.00%), Postives = 43/60 (71.67%), Query Frame = 1 
Query:    1 SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKD 180
            S+TNRL+ AKDHSS QINV  +DPV+G  TG+   Y L GYLR + E D ALT L+ KKD
Sbjct:   18 SYTNRLVSAKDHSSVQINVANVDPVTGIATGDYTPYCLCGYLRFKSEGDMALTTLITKKD 77
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BLAST of mRNA_Ecto-sp13_S_contig19524.6285.1 vs. uniprot
Match: A0A0P1AAL2_PLAHL (40S ribosomal protein S21 n=16 Tax=Peronosporaceae TaxID=4777 RepID=A0A0P1AAL2_PLAHL)

HSP 1 Score: 75.9 bits (185), Expect = 3.630e-14
Identity = 36/60 (60.00%), Postives = 46/60 (76.67%), Query Frame = 1 
Query:    1 SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKD 180
            SWTNR+L AKDH+S QINVG+++  +G FTGESD +ALAGY+R  GE D A+T L  + D
Sbjct:   18 SWTNRILAAKDHASVQINVGRVN-ANGVFTGESDTFALAGYIRHHGEGDIAITELSRQAD 76
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BLAST of mRNA_Ecto-sp13_S_contig19524.6285.1 vs. uniprot
Match: A0A067BLB8_SAPPC (40S ribosomal protein S21 n=5 Tax=Saprolegniaceae TaxID=4764 RepID=A0A067BLB8_SAPPC)

HSP 1 Score: 75.9 bits (185), Expect = 3.630e-14
Identity = 35/61 (57.38%), Postives = 46/61 (75.41%), Query Frame = 1 
Query:    1 SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKDE 183
            SWTNR+L AKDH+S QI V ++D  +G +TG +DVYALAGY+R +GE D ALT L  + +E
Sbjct:   18 SWTNRILAAKDHASVQIAVAQVD-ANGVYTGHNDVYALAGYIRNKGEGDHALTELARRAEE 77
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BLAST of mRNA_Ecto-sp13_S_contig19524.6285.1 vs. uniprot
Match: A0A1E7F2P4_9STRA (40S ribosomal protein S21 n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7F2P4_9STRA)

HSP 1 Score: 75.5 bits (184), Expect = 5.920e-14
Identity = 35/60 (58.33%), Postives = 43/60 (71.67%), Query Frame = 1 
Query:    1 SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKD 180
            +WTNRL++AKDHSS QINV  +DPV+G  TG++  Y L GY+R   E D ALT LV K D
Sbjct:   18 NWTNRLVEAKDHSSVQINVANVDPVTGMATGDATPYCLVGYIRMVSEGDMALTQLVKKND 77
[Close]
BLAST of mRNA_Ecto-sp13_S_contig19524.6285.1 vs. uniprot
Match: F0YCY8_AURAN (Uncharacterized protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YCY8_AURAN)

HSP 1 Score: 73.9 bits (180), Expect = 1.900e-13
Identity = 34/62 (54.84%), Postives = 45/62 (72.58%), Query Frame = 1 
Query:    1 SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADEALTCLVNKKDES 186
            SWTNRL++A D +S QINVG +D  +G +T     YAL+GY+R +GE+D ALT LV K DE+
Sbjct:   17 SWTNRLIEAFDKASVQINVGSIDANTGLYTNTYKTYALSGYVRNKGESDMALTDLVRKSDEA 78
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig19524.6285.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FW88_ECTSI3.610e-36100.0040S ribosomal protein S21 n=2 Tax=Ectocarpus TaxID... [more]
A0A1Z5KP36_FISSO2.790e-1657.8140S ribosomal protein S21 n=1 Tax=Fistulifera sola... [more]
G4ZUD9_PHYSP9.280e-1661.6740S ribosomal protein S21 n=7 Tax=Peronosporaceae ... [more]
K3WSU9_GLOUD4.920e-1561.6740S ribosomal protein S21 n=2 Tax=Pythiaceae TaxID... [more]
A0A448Z9N0_9STRA2.190e-1456.6740S ribosomal protein S21 n=1 Tax=Pseudo-nitzschia... [more]
A0A7S1UVR1_9STRA3.220e-1460.0040S ribosomal protein S21 n=1 Tax=Grammatophora oc... [more]
A0A0P1AAL2_PLAHL3.630e-1460.0040S ribosomal protein S21 n=16 Tax=Peronosporaceae... [more]
A0A067BLB8_SAPPC3.630e-1457.3840S ribosomal protein S21 n=5 Tax=Saprolegniaceae ... [more]
A0A1E7F2P4_9STRA5.920e-1458.3340S ribosomal protein S21 n=1 Tax=Fragilariopsis c... [more]
F0YCY8_AURAN1.900e-1354.84Uncharacterized protein (Fragment) n=1 Tax=Aureoco... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig19524contigEcto-sp13_S_contig19524:329..2456 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score131.7
Seed ortholog evalue1.2e-28
Seed eggNOG ortholog2880.D7FW88
Preferred nameRPS21
Model size835
KEGG rclassRC01978
KEGG koko:K02971,ko:K03093,ko:K03644,ko:K03955,ko:K08854,ko:K10900,ko:K13179,ko:K20168
KEGG TC3.D.1.6
KEGG ReactionR07767,R07768
KEGG Pathwayko00190,ko00785,ko01100,ko03010,ko04137,ko04714,ko04723,ko04932,ko05010,ko05012,ko05016,ko05202,map00190,map00785,map01100,map03010,map04137,map04714,map04723,map04932,map05010,map05012,map05016,map05202
KEGG ModuleM00146,M00177
Hectar predicted targeting categoryother localisation
GOsGO:0000184,GO:0000447,GO:0000460,GO:0000461,GO:0000462,GO:0000466,GO:0000469,GO:0000478,GO:0000479,GO:0000956,GO:0002181,GO:0002182,GO:0002376,GO:0002520,GO:0003674,GO:0003735,GO:0005198,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005783,GO:0005789,GO:0005791,GO:0005829,GO:0005840,GO:0005844,GO:0006139,GO:0006364,GO:0006396,GO:0006401,GO:0006402,GO:0006412,GO:0006413,GO:0006414,GO:0006518,GO:0006605,GO:0006612,GO:0006613,GO:0006614,GO:0006725,GO:0006807,GO:0006810,GO:0006886,GO:0006996,GO:0007275,GO:0008104,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009058,GO:0009059,GO:0009657,GO:0009658,GO:0009892,GO:0009987,GO:0010467,GO:0010468,GO:0010605,GO:0010629,GO:0012505,GO:0015031,GO:0015833,GO:0015935,GO:0016020,GO:0016043,GO:0016070,GO:0016071,GO:0016072,GO:0019222,GO:0019439,GO:0019538,GO:0022613,GO:0022626,GO:0022627,GO:0030490,GO:0030867,GO:0031090,GO:0031123,GO:0031125,GO:0031974,GO:0031981,GO:0031984,GO:0032501,GO:0032502,GO:0032991,GO:0033036,GO:0033365,GO:0034470,GO:0034613,GO:0034641,GO:0034645,GO:0034655,GO:0034660,GO:0042127,GO:0042175,GO:0042254,GO:0042274,GO:0042788,GO:0042886,GO:0043021,GO:0043022,GO:0043043,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043603,GO:0043604,GO:0043628,GO:0044085,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044260,GO:0044265,GO:0044267,GO:0044270,GO:0044271,GO:0044391,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044432,GO:0044444,GO:0044445,GO:0044446,GO:0044464,GO:0044877,GO:0045047,GO:0045184,GO:0046483,GO:0046700,GO:0046907,GO:0047485,GO:0048513,GO:0048519,GO:0048534,GO:0048542,GO:0048731,GO:0048732,GO:0048856,GO:0050789,GO:0050794,GO:0051179,GO:0051234,GO:0051641,GO:0051649,GO:0060255,GO:0065007,GO:0070013,GO:0070727,GO:0070972,GO:0071702,GO:0071704,GO:0071705,GO:0071840,GO:0072594,GO:0072599,GO:0072657,GO:0090150,GO:0090304,GO:0090305,GO:0090501,GO:0090502,GO:0098552,GO:0098554,GO:0098556,GO:0098562,GO:0098588,GO:0098827,GO:1901360,GO:1901361,GO:1901564,GO:1901566,GO:1901575,GO:1901576,GO:1990904
Exons3
EggNOG free text desc.endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
EggNOG OGsKOG3486@1,KOG3486@2759
Ec32 ortholog descriptionRibosomal protein S21e
Ec32 orthologEc-01_003800.1
EC2.7.11.1,2.8.1.8,3.6.4.12,3.6.4.13
Cds size195
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01610,ko00000,ko00001,ko00002,ko01000,ko01001,ko03009,ko03011,ko03021,ko03032,ko03041,ko03400,ko04131
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462989.5867975-CDS-Ecto-sp13_S_contig19524:328..3761681462989.5867975-CDS-Ecto-sp13_S_contig19524:328..376Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19524 329..376 +
1681462989.598481-CDS-Ecto-sp13_S_contig19524:1017..11041681462989.598481-CDS-Ecto-sp13_S_contig19524:1017..1104Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19524 1018..1104 +
1681462989.6065207-CDS-Ecto-sp13_S_contig19524:1758..18181681462989.6065207-CDS-Ecto-sp13_S_contig19524:1758..1818Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19524 1759..1818 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462989.615206-UTR-Ecto-sp13_S_contig19524:1818..24561681462989.615206-UTR-Ecto-sp13_S_contig19524:1818..2456Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig19524 1819..2456 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig19524.6285.1prot_Ecto-sp13_S_contig19524.6285.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig19524 329..1818 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig19524.6285.1

>prot_Ecto-sp13_S_contig19524.6285.1 ID=prot_Ecto-sp13_S_contig19524.6285.1|Name=mRNA_Ecto-sp13_S_contig19524.6285.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=65bp
SWTNRLLQAKDHSSTQINVGKLDPVSGTFTGESDVYALAGYLRGRGEADE
ALTCLVNKKDESEL*
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mRNA from alignment at Ecto-sp13_S_contig19524:329..2456+

Legend: CDSpolypeptideUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig19524.6285.1 ID=mRNA_Ecto-sp13_S_contig19524.6285.1|Name=mRNA_Ecto-sp13_S_contig19524.6285.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2128bp|location=Sequence derived from alignment at Ecto-sp13_S_contig19524:329..2456+ (Ectocarpus species13 EcNAP12_S_4_19m)
TCCTGGACCAACCGCCTCTTGCAGGCCAAGGACCACTCCTCCACCCAGGT
CAGTGGCTCACTTGGTCTTTTTCGTATCCTCCTCCGCCTTCGGCTCGTAC
GTTGCTTCACAAAGTATGACGGGAGTTCACAGCAGCATCGTACCTGCCAG
CGTTGGTGTCGCGCCCGTTCTTAAGTGGCAATTAGTGTCCATTTTTGGGC
TGTCTGCGCTACAACTTTCCGGCCCCGGTTTACCGGTTGGATACTTGGGT
GTCTGTTCATCACGCACCCAGCCTCTGCCGATGGATGCTGTACGAGCTTT
CTGCCGCTTTGCATGGCGCGATGTCATGGGAAGATATGTATGCGGCATTT
TTACCGGTAGCGCTTCGTGAAAGGACTGAAGTCAGCCGCCTGGCGGGTCG
AGCGTTTCTGTCTGCCTCAACTACCCGTGCACAGAAGGGGTAGCAGCGTC
ACTGATGTTCTCTGGCACGAAATACGCCCCCCAGGAAGTAGCTTTGACCT
GGCGCATAGGCTGAGCAGCGTGTACCCGCCGGGCAAGGAGCGGTGAAAAT
TTAAAACCCCAACCACCCATGATCCACTGCTGGCTAATGAAGTCCGGCGC
CCGTCAAGTTCGGTGCTCTGTTCCTTTGTGCGTTCTCTTTCCTCTTGATC
TCAAACTTACCGACCGGTGCTCTTGCTCCTTGTGATCAGATCAACGTGGG
CAAGTTGGACCCCGTTTCGGGAACCTTCACCGGAGAGTCGGACGTGTACG
CGCTGGCTGGTTACTTGCGCGGCAGGGTGAGATTAGGTTGAGAGTAGAGA
CCAATCGCCCAGACCGATTTACCACCATTGCCTTTTTCGTAGCGCCTTTG
GTTCCGGTTTGTCTGTGGAATGGCGGCGTTCCTAGTGAGTATGTGGAAGG
CTGCGTGTGTTCGTATTAATGCCCTCTCAAGTTGGAAGTGGTATGTTCGT
GGAGGGTCACCCTCATGCCGTAGTGGCATCACGCTGCGAAGGTTCGGAAG
CTTGAAAGGTTTCAAGATGCCGAGGTTGGCTGAGACGAAGCGCCCATCTC
CAGATCGAATTGGTGTGACTCGGTCTGTACTTCCGATCTGGGATGCAACC
AATTTCGGGCCAAGTCAGCCTCAAAGGCCTTGAAGCTTGTAACAATGTGG
CAGTGTTTGGTTCGGTCTGCTGCGTCACACTGGAGCGTGTCCTGCTGCTG
TCCTTCGTACCTTGAGCTGCAGTCGTTGCATGTCATGTTCTATGCATGAT
TGTATCCCCCTGTCGAGGGCCGTCCGGCTGGCACCCGACGTCCCTACTGT
GCATTCATCATCGTGTTCTTGTTGGAACCGCTTCCACAACCAAGCCCCGT
CTGCGTGTGTTCATGAAAACATCCCCTGACCGCCCTCACACTGGCGTGTG
TTCGTGACCCCCCCCCTTTTTTTTTGGCAGGGTGAGGCCGACGAGGCGCT
GACGTGCCTTGTCAACAAGAAGGACGAGTCGGAGCTCTGAGATGCGCAAA
GGCGTCGCGCTTGCTGCGGGGGTGGGAGAGGACGCGGAACAGTCATACCG
CGACGCGTTTTTCTCTTGAGCATTTCGGATGACAATCCGCCATCGCCGAA
CGACGGCTGTTTCTTGGTAGTGCAAGCGAAGTTTTTCGGTTCATTCAAGC
GTTGGTGGTAAAAAAAAGGGCGAAACTGCTGGATCTTTGATGGAGGTAAT
CCGAGGTCCCTAACCGCCCATGTGTCGCGTTTGATTTTCTCCTGTGTTTC
AGCCGTCTACCCTTTGGGAGGGCTTGCCGGGCAGAATTTCCGGTCGTGTG
CTGTCTACCGATGTTTTCGTCGGCATTGGGCCTGACACTTGAGATCCTGC
GTGCTTGCTAGTGCGGCGAAACACAGTTCCCACATCGGCGTCCCGGGCAT
TTAGAAAAGCAGTGAGGTCCATGGCGTCCCTCACCATCACTTTTGTCATA
GTTGGACTCGGGCGCCTACTCCGTAACGAACAACTTCGTGGTGTCCCGAG
TCGGAAAAGACGGTAGCGCGCGCTGATCGTTTCGCGGCACGTCGACAAGC
GAAAACATACAAAACATACACGATCCATTGTTTGCAGATGGATGAGAGAG
CAATTCGATGCTGTTTGCCTGACGCACC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig19524:329..2456+

>mRNA_Ecto-sp13_S_contig19524.6285.1 ID=mRNA_Ecto-sp13_S_contig19524.6285.1|Name=mRNA_Ecto-sp13_S_contig19524.6285.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=195bp|location=Sequence derived from alignment at Ecto-sp13_S_contig19524:329..2456+ (Ectocarpus species13 EcNAP12_S_4_19m)
TCCTGGACCAACCGCCTCTTGCAGGCCAAGGACCACTCCTCCACCCAGAT
CAACGTGGGCAAGTTGGACCCCGTTTCGGGAACCTTCACCGGAGAGTCGG
ACGTGTACGCGCTGGCTGGTTACTTGCGCGGCAGGGGTGAGGCCGACGAG
GCGCTGACGTGCCTTGTCAACAAGAAGGACGAGTCGGAGCTCTGA
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