mRNA_Ecto-sp13_S_contig19226.6149.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig19226.6149.1
Unique NamemRNA_Ecto-sp13_S_contig19226.6149.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig19226.6149.1 vs. uniprot
Match: A0A6H5JXW2_9PHAE (Glutamine amidotransferase type-2 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JXW2_9PHAE)

HSP 1 Score: 207 bits (527), Expect = 2.050e-62
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 1 
Query:    1 HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPFK 288
            HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPFK
Sbjct:   10 HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPFK 105
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BLAST of mRNA_Ecto-sp13_S_contig19226.6149.1 vs. uniprot
Match: D8LKF2_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LKF2_ECTSI)

HSP 1 Score: 207 bits (527), Expect = 1.630e-60
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 1 
Query:    1 HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPFK 288
            HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPFK
Sbjct:   16 HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPFK 111
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BLAST of mRNA_Ecto-sp13_S_contig19226.6149.1 vs. uniprot
Match: A0A482RVP5_9ARCH (Class II glutamine amidotransferase (Fragment) n=1 Tax=archaeon TaxID=1906665 RepID=A0A482RVP5_9ARCH)

HSP 1 Score: 111 bits (278), Expect = 7.480e-29
Identity = 56/102 (54.90%), Postives = 70/102 (68.63%), Query Frame = 1 
Query:    1 HSLVFQSRDASFHPGCADK---RNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGN---VNHENTHPFK 288
            +SL++QSRDA++HPG  DK   RNI VNGDGFG+ WY    + +GGC  KF TPAWSN NL +I + V S LV AH+RAAS G   +     V+HEN HPF+
Sbjct:   23 NSLIYQSRDAAYHPGVTDKTHRRNILVNGDGFGVCWYG-ENVSKGGCCFKFVTPAWSNGNLRSIGEHVASHLVCAHIRAASSGHDPLHERVAVSHENCHPFR 123
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BLAST of mRNA_Ecto-sp13_S_contig19226.6149.1 vs. uniprot
Match: A0A7S0DVN1_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Amorphochlora amoebiformis TaxID=1561963 RepID=A0A7S0DVN1_9EUKA)

HSP 1 Score: 94.7 bits (234), Expect = 5.220e-22
Identity = 51/103 (49.51%), Postives = 63/103 (61.17%), Query Frame = 1 
Query:    1 HSLVFQSRDASFHPGCA-------DKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPFK 288
            +S++ QSRDA F PG         DKRN RVN DGFG+AWYT  R  +  C+ K   P+W ++NLL +A  VES  +FAHVRAAS G   V     +N HPFK
Sbjct:   21 NSIINQSRDAQFLPGLTYENQAITDKRNNRVNADGFGVAWYTKGR--KNACLYKSTQPSWGDSNLLELADHVESPTIFAHVRAASMGVASV-----QNCHPFK 116
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BLAST of mRNA_Ecto-sp13_S_contig19226.6149.1 vs. uniprot
Match: A0A1E4EKC6_9BACT (Glutamine amidotransferase type-2 domain-containing protein n=1 Tax=bacterium SCN 62-11 TaxID=1660156 RepID=A0A1E4EKC6_9BACT)

HSP 1 Score: 80.1 bits (196), Expect = 8.600e-16
Identity = 43/96 (44.79%), Postives = 59/96 (61.46%), Query Frame = 1 
Query:    1 HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPFK 288
            HS++ QS    +H   + +R   +NGDGFG+AWY PR + +   + K  TPAWSN NL  +A+ VES  +FAHVRAA+ G+     V+  N HPF 
Sbjct:   23 HSIIHQS----YH---SRERPEPLNGDGFGLAWYVPR-LSQSAALFKDTTPAWSNENLRQVARVVESDCIFAHVRAATGGSP----VSRLNCHPFS 106
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BLAST of mRNA_Ecto-sp13_S_contig19226.6149.1 vs. uniprot
Match: UPI001643DCB6 (class II glutamine amidotransferase n=1 Tax=unclassified Stappia TaxID=2629676 RepID=UPI001643DCB6)

HSP 1 Score: 78.2 bits (191), Expect = 3.600e-15
Identity = 46/95 (48.42%), Postives = 58/95 (61.05%), Query Frame = 1 
Query:    1 HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPF 285
            HSL+ QS+DA        +  +RVNGDGFGIAWY    + E G + K   PAW++TNL +I + V SGL  AHVRAA+ GA      + EN HPF
Sbjct:   23 HSLLVQSQDAQ-------EAKLRVNGDGFGIAWYG--HLPEPG-LFKDVLPAWADTNLPSICRLVRSGLFLAHVRAATTGAS-----SRENCHPF 102
[Close]
BLAST of mRNA_Ecto-sp13_S_contig19226.6149.1 vs. uniprot
Match: F2J3M2_POLGS (Glutamine amidotransferase, class-II n=1 Tax=Polymorphum gilvum (strain LMG 25793 / CGMCC 1.9160 / SL003B-26A1) TaxID=991905 RepID=F2J3M2_POLGS)

HSP 1 Score: 75.5 bits (184), Expect = 3.300e-14
Identity = 44/95 (46.32%), Postives = 57/95 (60.00%), Query Frame = 1 
Query:    1 HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPF 285
            HSL+ QS+DA        +  +RVNGDGFGIAWY    + E G + K   PAWS+TNL ++ + V S L  AHVRAA+ GA      + +N HPF
Sbjct:   23 HSLLVQSQDAQ-------EAKLRVNGDGFGIAWYG--HLPEPG-LFKDVLPAWSDTNLPSVCRLVRSRLFLAHVRAATTGAT-----SRDNCHPF 102
[Close]
BLAST of mRNA_Ecto-sp13_S_contig19226.6149.1 vs. uniprot
Match: Q5A5C1_CANAL (Glutamine amidotransferase subunit n=9 Tax=Candida TaxID=1535326 RepID=Q5A5C1_CANAL)

HSP 1 Score: 75.9 bits (185), Expect = 4.910e-14
Identity = 43/96 (44.79%), Postives = 56/96 (58.33%), Query Frame = 1 
Query:    1 HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPFK 288
            HS++ QS D+       D RN  VNGDGFG+ +Y+  R     C+ K  TPAW+N NL N+A+  ES LVFAHVRA++ G +        N HPF 
Sbjct:   24 HSIINQSFDSRLR---LDMRNP-VNGDGFGVGYYSENRHP---CIFKAITPAWNNVNLNNLAECTESSLVFAHVRASTQGVLA-----ETNCHPFS 107
[Close]
BLAST of mRNA_Ecto-sp13_S_contig19226.6149.1 vs. uniprot
Match: A0A285SU75_9HYPH (Glutamine amidotransferase n=6 Tax=Stappia TaxID=152161 RepID=A0A285SU75_9HYPH)

HSP 1 Score: 75.1 bits (183), Expect = 4.950e-14
Identity = 45/95 (47.37%), Postives = 57/95 (60.00%), Query Frame = 1 
Query:    1 HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPF 285
            HSL+ QS+DA        +  +RVNGDGFGIAWY    + E G + K   PAW++TNL +I + V S L  AHVRAA+ GA      + EN HPF
Sbjct:   23 HSLLVQSQDAQ-------EAKLRVNGDGFGIAWYG--HLPEPG-LFKDVLPAWADTNLPSICRLVRSRLFLAHVRAATTGAS-----SRENCHPF 102
[Close]
BLAST of mRNA_Ecto-sp13_S_contig19226.6149.1 vs. uniprot
Match: A0A2H9SP59_9GAMM (Class II glutamine amidotransferase n=1 Tax=Legionella sp. TaxID=459 RepID=A0A2H9SP59_9GAMM)

HSP 1 Score: 75.1 bits (183), Expect = 5.790e-14
Identity = 39/79 (49.37%), Postives = 47/79 (59.49%), Query Frame = 1 
Query:   49 ADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPTPAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPF 285
            A +     NGDGFG+ WY P+ I E         PAWS+ NLL++ K +ESGL FAHVRAAS     +G VN  N HPF
Sbjct:   32 AKETTTPTNGDGFGVGWYVPK-ISEHPAAFLSVFPAWSDENLLHLTKKIESGLFFAHVRAAS-----IGGVNTYNCHPF 104
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig19226.6149.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JXW2_9PHAE2.050e-62100.00Glutamine amidotransferase type-2 domain-containin... [more]
D8LKF2_ECTSI1.630e-60100.00Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A482RVP5_9ARCH7.480e-2954.90Class II glutamine amidotransferase (Fragment) n=1... [more]
A0A7S0DVN1_9EUKA5.220e-2249.51Hypothetical protein (Fragment) n=1 Tax=Amorphochl... [more]
A0A1E4EKC6_9BACT8.600e-1644.79Glutamine amidotransferase type-2 domain-containin... [more]
UPI001643DCB63.600e-1548.42class II glutamine amidotransferase n=1 Tax=unclas... [more]
F2J3M2_POLGS3.300e-1446.32Glutamine amidotransferase, class-II n=1 Tax=Polym... [more]
Q5A5C1_CANAL4.910e-1444.79Glutamine amidotransferase subunit n=9 Tax=Candida... [more]
A0A285SU75_9HYPH4.950e-1447.37Glutamine amidotransferase n=6 Tax=Stappia TaxID=1... [more]
A0A2H9SP59_9GAMM5.790e-1449.37Class II glutamine amidotransferase n=1 Tax=Legion... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig19226contigEcto-sp13_S_contig19226:182..1032 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score210.3
Seed ortholog evalue4.3e-52
Seed eggNOG ortholog2880.D8LKF2
Preferred nameDUG3
Model size315
KEGG rclassRC00408
KEGG koko:K01836,ko:K14262,ko:K18802
KEGG ReactionR08193
KEGG Pathwayko00520,ko01100,ko01130,map00520,map01100,map01130
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004044,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006508,GO:0006518,GO:0006575,GO:0006749,GO:0006751,GO:0006790,GO:0006807,GO:0008150,GO:0008152,GO:0008233,GO:0008238,GO:0008242,GO:0009056,GO:0009987,GO:0016740,GO:0016757,GO:0016763,GO:0016787,GO:0019538,GO:0032991,GO:0034641,GO:0042219,GO:0043170,GO:0043171,GO:0043603,GO:0044237,GO:0044238,GO:0044248,GO:0044273,GO:0044424,GO:0044464,GO:0051186,GO:0051187,GO:0061672,GO:0070011,GO:0071704,GO:0140096,GO:1901564,GO:1901565,GO:1901575,GO:1902494,GO:1905368
Exons2
EggNOG free text desc.glutamine-fructose-6-phosphate transaminase (isomerizing) activity
EggNOG OGsCOG0449@1,KOG1268@2759
EC5.4.2.3
Cds size315
COG Functional cat.M
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01002
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462978.7396028-CDS-Ecto-sp13_S_contig19226:181..3981681462978.7396028-CDS-Ecto-sp13_S_contig19226:181..398Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19226 182..398 -
1681462978.759346-CDS-Ecto-sp13_S_contig19226:934..10321681462978.759346-CDS-Ecto-sp13_S_contig19226:934..1032Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19226 935..1032 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig19226.6149.1prot_Ecto-sp13_S_contig19226.6149.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig19226 182..1032 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig19226.6149.1

>prot_Ecto-sp13_S_contig19226.6149.1 ID=prot_Ecto-sp13_S_contig19226.6149.1|Name=mRNA_Ecto-sp13_S_contig19226.6149.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=105bp
HSLVFQSRDASFHPGCADKRNIRVNGDGFGIAWYTPRRIEEGGCVVKFPT
PAWSNTNLLNIAKFVESGLVFAHVRAASDGAVLVGNVNHENTHPFKVLLV
FLSL*
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mRNA from alignment at Ecto-sp13_S_contig19226:182..1032-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig19226.6149.1 ID=mRNA_Ecto-sp13_S_contig19226.6149.1|Name=mRNA_Ecto-sp13_S_contig19226.6149.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=851bp|location=Sequence derived from alignment at Ecto-sp13_S_contig19226:182..1032- (Ectocarpus species13 EcNAP12_S_4_19m)
CACTCGTTGGTTTTCCAGTCGAGGGACGCGTCTTTTCACCCCGGGTGTGC
CGACAAGAGGAACATCCGCGTCAACGGCGATGGCTTCGGAATCGCTTGGT
GAGACATTCAAAGTGCCGAGTGCTGTTGAATGGGGCTTGCGGCACCACCT
TTGGAGCTCCTGTTCCTTGTTTTGGGAGCAACAAAAACATGACTCTCCTC
CTTTTCTCAGCCTGGTCTTATGGCACGATGGAATACTGTGTTGTGTGGAG
GAGCTTCCGTCGAACATCCCGGAAAAGGGGGGCCCTGTGAGGGTTTGCTC
AACCAGAGGGGATCACTGAAAGGACAAAGGGATCGCTGCGCCTCGTGGGC
TTTGAACGACTTTTATCGCCCCACGAAGCAAAGCGGAAGGGCCTGTAGAC
TCCCTGCCTCCATCGCCTGACAGATTGCCGTCGAGATGCTGCCTTATTTC
GCCTCCATGGCGGACTTCACCAGGCCTTGAACAACACTTTGGATAGATAC
CACACGGGTGGTCTTTTGCTGTGGCTGAACGTGTACGAGCGAAACTGCTG
TTTGAGCGATCCCACGAAACAAATTTGGCCCCCTACCCCACCCCCATCCC
TATCCCCCACCGCTGGGGCGACGTTTTACCGTAGGTACACCCCGAGACGG
ATCGAGGAAGGGGGATGCGTGGTAAAGTTTCCTACCCCGGCCTGGTCCAA
CACCAACCTTCTCAACATCGCCAAGTTCGTTGAGTCAGGCCTCGTGTTCG
CACACGTTCGGGCGGCGTCGGATGGGGCCGTGCTGGTGGGGAACGTGAAC
CACGAGAACACCCACCCTTTCAAGGTGCTTTTGGTCTTCTTAAGCTTATA
G
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig19226:182..1032-

>mRNA_Ecto-sp13_S_contig19226.6149.1 ID=mRNA_Ecto-sp13_S_contig19226.6149.1|Name=mRNA_Ecto-sp13_S_contig19226.6149.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=315bp|location=Sequence derived from alignment at Ecto-sp13_S_contig19226:182..1032- (Ectocarpus species13 EcNAP12_S_4_19m)
CACTCGTTGGTTTTCCAGTCGAGGGACGCGTCTTTTCACCCCGGGTGTGC
CGACAAGAGGAACATCCGCGTCAACGGCGATGGCTTCGGAATCGCTTGGT
ACACCCCGAGACGGATCGAGGAAGGGGGATGCGTGGTAAAGTTTCCTACC
CCGGCCTGGTCCAACACCAACCTTCTCAACATCGCCAAGTTCGTTGAGTC
AGGCCTCGTGTTCGCACACGTTCGGGCGGCGTCGGATGGGGCCGTGCTGG
TGGGGAACGTGAACCACGAGAACACCCACCCTTTCAAGGTGCTTTTGGTC
TTCTTAAGCTTATAG
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