mRNA_Ecto-sp13_S_contig17863.5435.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig17863.5435.1
Unique NamemRNA_Ecto-sp13_S_contig17863.5435.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
BLAST of mRNA_Ecto-sp13_S_contig17863.5435.1 vs. uniprot
Match: D7G637_ECTSI (Homocysteine S-methyltransferase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G637_ECTSI)

HSP 1 Score: 176 bits (447), Expect = 8.540e-52
Identity = 89/93 (95.70%), Postives = 91/93 (97.85%), Query Frame = 1
Query:    1 EEENAKQHQQPLSGSEGRPHRRRLRPLVAASLGCYGAVLADGSEYRGDYVDTPAGSLKEFHARRLEILARADGVDMVVFETVPCLAEVRAILS 279
            EEENAKQHQQP+ GS+GR HRRRLRPLVAASLGCYGAVLADGSEYRGDYVDTPAGSLKEFHARRLEILARADGVDMVVFETVPCLAEVRAILS
Sbjct:  139 EEENAKQHQQPVRGSDGRRHRRRLRPLVAASLGCYGAVLADGSEYRGDYVDTPAGSLKEFHARRLEILARADGVDMVVFETVPCLAEVRAILS 231          
BLAST of mRNA_Ecto-sp13_S_contig17863.5435.1 vs. uniprot
Match: A0A6H5KFQ7_9PHAE (Hcy-binding domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KFQ7_9PHAE)

HSP 1 Score: 172 bits (437), Expect = 3.990e-50
Identity = 86/87 (98.85%), Postives = 86/87 (98.85%), Query Frame = 1
Query:    1 EEENAKQHQQPLSGSEGRPHRRRLRPLVAASLGCYGAVLADGSEYRGDYVDTPAGSLKEFHARRLEILARADGVDMVVFETVPCLAE 261
            EEEN KQHQQPLSGSEGRPHRRRLRPLVAASLGCYGAVLADGSEYRGDYVDTPAGSLKEFHARRLEILARADGVDMVVFETVPCLAE
Sbjct:  169 EEENPKQHQQPLSGSEGRPHRRRLRPLVAASLGCYGAVLADGSEYRGDYVDTPAGSLKEFHARRLEILARADGVDMVVFETVPCLAE 255          
BLAST of mRNA_Ecto-sp13_S_contig17863.5435.1 vs. uniprot
Match: I0YMA9_COCSC (Homocysteine S-methyltransferase AtHMT-1 n=1 Tax=Coccomyxa subellipsoidea (strain C-169) TaxID=574566 RepID=I0YMA9_COCSC)

HSP 1 Score: 82.0 bits (201), Expect = 1.090e-16
Identity = 41/71 (57.75%), Postives = 51/71 (71.83%), Query Frame = 1
Query:   64 RRLRPLVAASLGCYGAVLADGSEYRGDYVDTPAGS-LKEFHARRLEILARADGVDMVVFETVPCLAEVRAI 273
            +RLRPLVA S G YGA LADGSEY G Y +  +   L +FH +RL+ +  A GVD++ FETVPCL E+RAI
Sbjct:   91 KRLRPLVAFSAGPYGAALADGSEYDGSYAERVSEEQLMDFHRQRLQAIVGAPGVDLIAFETVPCLKELRAI 161          
BLAST of mRNA_Ecto-sp13_S_contig17863.5435.1 vs. uniprot
Match: A0A433P5G0_9FUNG (Homocysteine S-methyltransferase n=1 Tax=Endogone sp. FLAS-F59071 TaxID=2340872 RepID=A0A433P5G0_9FUNG)

HSP 1 Score: 82.8 bits (203), Expect = 1.190e-16
Identity = 37/68 (54.41%), Postives = 48/68 (70.59%), Query Frame = 1
Query:   70 LRPLVAASLGCYGAVLADGSEYRGDYVDTPAGSLKEFHARRLEILARADGVDMVVFETVPCLAEVRAI 273
            +RPLVA SLGCYGA+LA+GSEY G+Y+D     L +FH +RL +     G+D+V FET+P L E  AI
Sbjct:  164 VRPLVALSLGCYGAMLANGSEYTGEYLDATTEQLVDFHRQRLRVFFAEPGIDLVAFETIPSLQEAEAI 231          
BLAST of mRNA_Ecto-sp13_S_contig17863.5435.1 vs. uniprot
Match: UPI001749A229 (homocysteine S-methyltransferase n=1 Tax=Nocardioides flavus Wang et al. 2016 TaxID=2058780 RepID=UPI001749A229)

HSP 1 Score: 80.9 bits (198), Expect = 3.080e-16
Identity = 42/68 (61.76%), Postives = 50/68 (73.53%), Query Frame = 1
Query:   82 VAASLGCYGAVLADGSEYRGDYVDTPAGSLKEFHARRLEILARA--DGVDMVVFETVPCLAEVRAILS 279
            VAAS+G YGAVLADGSEYRGDY D     L+ FH  RLE+LA    DG D++  ET+PCLAEV A+L+
Sbjct:  110 VAASVGPYGAVLADGSEYRGDY-DLDVAGLRAFHRPRLEVLASTVGDGADVLAVETIPCLAEVEAVLA 176          
BLAST of mRNA_Ecto-sp13_S_contig17863.5435.1 vs. uniprot
Match: A0A835ZJM3_9STRA (Homocysteine S-methyltransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZJM3_9STRA)

HSP 1 Score: 80.9 bits (198), Expect = 5.920e-16
Identity = 45/72 (62.50%), Postives = 53/72 (73.61%), Query Frame = 1
Query:   61 RRRLRPLVAASLGCYGAVLADGSEYRGDYVDTPAGSLKEFHARRLEILARADGVDMVVFETVPCLAEVRAIL 276
            RRRLRPLVAAS+G YGA LADGSEY+G+Y  T    L ++H  RL  LA A G D+V  ETVPCLAE RA++
Sbjct:  140 RRRLRPLVAASVGSYGAFLADGSEYKGNYGMT-IEQLMDWHRPRLRALA-ATGADLVACETVPCLAEARALV 209          
BLAST of mRNA_Ecto-sp13_S_contig17863.5435.1 vs. uniprot
Match: A0A7Y9KQW4_9ACTN (Homocysteine S-methyltransferase n=1 Tax=Nocardioides cavernae TaxID=1921566 RepID=A0A7Y9KQW4_9ACTN)

HSP 1 Score: 80.1 bits (196), Expect = 6.360e-16
Identity = 42/68 (61.76%), Postives = 50/68 (73.53%), Query Frame = 1
Query:   82 VAASLGCYGAVLADGSEYRGDYVDTPAGSLKEFHARRLEILARA--DGVDMVVFETVPCLAEVRAILS 279
            VAAS+G YGAVLADGSEYRGDY D     L+ FH  RL++LA    DG D++  ETVPCLAEV A+L+
Sbjct:  114 VAASVGPYGAVLADGSEYRGDY-DLDVAGLRAFHRPRLDVLASTVGDGADVLAVETVPCLAEVEAVLA 180          
BLAST of mRNA_Ecto-sp13_S_contig17863.5435.1 vs. uniprot
Match: A0A835SWU2_CHLIN (Hcy-binding domain-containing protein n=1 Tax=Chlamydomonas incerta TaxID=51695 RepID=A0A835SWU2_CHLIN)

HSP 1 Score: 80.9 bits (198), Expect = 6.570e-16
Identity = 40/72 (55.56%), Postives = 48/72 (66.67%), Query Frame = 1
Query:   67 RLRPLVAASLGCYGAVLADGSEYRGDYVDT-PAGSLKEFHARRLEILARADGVDMVVFETVPCLAEVRAILS 279
            R RPL+A S G YGA LADGSE+RGDY D+ PA  L  FH  RLE +     +D++ FETVPCL E  AI+ 
Sbjct:  155 RRRPLIAYSCGSYGAYLADGSEFRGDYADSVPADELMGFHRTRLEPVRSRPEIDLIAFETVPCLREAEAIVE 226          
BLAST of mRNA_Ecto-sp13_S_contig17863.5435.1 vs. uniprot
Match: A0A7Y3DZB4_9GAMM (Homocysteine S-methyltransferase n=1 Tax=Xanthomonadales bacterium TaxID=2006849 RepID=A0A7Y3DZB4_9GAMM)

HSP 1 Score: 80.1 bits (196), Expect = 7.160e-16
Identity = 40/74 (54.05%), Postives = 53/74 (71.62%), Query Frame = 1
Query:   58 HRRRLRPLVAASLGCYGAVLADGSEYRGDYVDTPAGSLKEFHARRLEILARADGVDMVVFETVPCLAEVRAILS 279
            HRRRLRPLVAAS+G YGA+L DGSEY G+Y D    +L++FH  RL++LA +D  D++ FET+P   E   +L 
Sbjct:  116 HRRRLRPLVAASIGPYGAILHDGSEYHGNY-DLNRRALRDFHRSRLDVLAESDA-DLLAFETIPSQEEAEILLE 187          
BLAST of mRNA_Ecto-sp13_S_contig17863.5435.1 vs. uniprot
Match: A0A8J9SJ70_9CHLO (Homocysteine S-methyltransferase 1 n=1 Tax=Coccomyxa sp. Obi TaxID=2315456 RepID=A0A8J9SJ70_9CHLO)

HSP 1 Score: 79.3 bits (194), Expect = 7.560e-16
Identity = 39/70 (55.71%), Postives = 49/70 (70.00%), Query Frame = 1
Query:   67 RLRPLVAASLGCYGAVLADGSEYRGDYVD-TPAGSLKEFHARRLEILARADGVDMVVFETVPCLAEVRAI 273
            R+RPLVA S G YGA LADGSEY G Y +      L +FH +RL+ +A   G+D++ FETVPCL E+RAI
Sbjct:   85 RVRPLVAFSAGPYGAALADGSEYDGSYAERVTEEQLVDFHRQRLQAIAGQPGIDLIAFETVPCLKELRAI 154          
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig17863.5435.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G637_ECTSI8.540e-5295.70Homocysteine S-methyltransferase n=1 Tax=Ectocarpu... [more]
A0A6H5KFQ7_9PHAE3.990e-5098.85Hcy-binding domain-containing protein n=1 Tax=Ecto... [more]
I0YMA9_COCSC1.090e-1657.75Homocysteine S-methyltransferase AtHMT-1 n=1 Tax=C... [more]
A0A433P5G0_9FUNG1.190e-1654.41Homocysteine S-methyltransferase n=1 Tax=Endogone ... [more]
UPI001749A2293.080e-1661.76homocysteine S-methyltransferase n=1 Tax=Nocardioi... [more]
A0A835ZJM3_9STRA5.920e-1662.50Homocysteine S-methyltransferase n=1 Tax=Tribonema... [more]
A0A7Y9KQW4_9ACTN6.360e-1661.76Homocysteine S-methyltransferase n=1 Tax=Nocardioi... [more]
A0A835SWU2_CHLIN6.570e-1655.56Hcy-binding domain-containing protein n=1 Tax=Chla... [more]
A0A7Y3DZB4_9GAMM7.160e-1654.05Homocysteine S-methyltransferase n=1 Tax=Xanthomon... [more]
A0A8J9SJ70_9CHLO7.560e-1655.71Homocysteine S-methyltransferase 1 n=1 Tax=Coccomy... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig17863contigEcto-sp13_S_contig17863:3315..3595 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score177.9
Seed ortholog evalue2.1e-42
Seed eggNOG ortholog2880.D7G637
Preferred nameHMT3
Model size281
KEGG rclassRC00003,RC00035,RC00496
KEGG koko:K00544,ko:K00547,ko:K17759
KEGG ReactionR00650,R02821
KEGG Pathwayko00260,ko00270,ko01100,ko01110,map00260,map00270,map01100,map01110
Hectar predicted targeting categoryother localisation
GOsGO:0000096,GO:0000097,GO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005886,GO:0006082,GO:0006520,GO:0006534,GO:0006555,GO:0006575,GO:0006790,GO:0006807,GO:0008150,GO:0008152,GO:0008168,GO:0008172,GO:0008652,GO:0008757,GO:0008898,GO:0009058,GO:0009066,GO:0009067,GO:0009069,GO:0009086,GO:0009987,GO:0016020,GO:0016053,GO:0016740,GO:0016741,GO:0019752,GO:0032259,GO:0033477,GO:0033528,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0044237,GO:0044238,GO:0044249,GO:0044272,GO:0044281,GO:0044283,GO:0044424,GO:0044464,GO:0046394,GO:0071704,GO:0071944,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901607
Exons1
EggNOG free text desc.betaine-homocysteine S-methyltransferase activity
EggNOG OGsCOG0646@1,KOG1579@2759
EC2.1.1.10,2.1.1.5,5.1.99.6
Cds size279
COG Functional cat.E
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462925.2381606-CDS-Ecto-sp13_S_contig17863:3316..35951681462925.2381606-CDS-Ecto-sp13_S_contig17863:3316..3595Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig17863 3317..3595 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig17863.5435.1prot_Ecto-sp13_S_contig17863.5435.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig17863 3317..3595 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig17863.5435.1

>prot_Ecto-sp13_S_contig17863.5435.1 ID=prot_Ecto-sp13_S_contig17863.5435.1|Name=mRNA_Ecto-sp13_S_contig17863.5435.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=93bp
EEENAKQHQQPLSGSEGRPHRRRLRPLVAASLGCYGAVLADGSEYRGDYV
DTPAGSLKEFHARRLEILARADGVDMVVFETVPCLAEVRAILS
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mRNA from alignment at Ecto-sp13_S_contig17863:3315..3595-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig17863.5435.1 ID=mRNA_Ecto-sp13_S_contig17863.5435.1|Name=mRNA_Ecto-sp13_S_contig17863.5435.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=281bp|location=Sequence derived from alignment at Ecto-sp13_S_contig17863:3315..3595- (Ectocarpus species13 EcNAP12_S_4_19m)
GAAGAAGAGAATGCCAAGCAACACCAACAGCCGCTCAGCGGGTCAGAGGG ACGACCACACCGACGACGACTGCGGCCGCTTGTTGCCGCCAGCCTTGGGT GCTACGGAGCAGTGCTCGCCGACGGTTCGGAGTACCGAGGGGACTACGTC GACACGCCGGCGGGAAGCCTGAAGGAGTTTCACGCCAGGCGGCTGGAGAT CCTGGCGAGGGCGGACGGTGTCGACATGGTCGTGTTCGAGACGGTTCCTT GCCTTGCGGAAGTGCGCGCCATCCTCTCGCT
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig17863:3315..3595-

>mRNA_Ecto-sp13_S_contig17863.5435.1 ID=mRNA_Ecto-sp13_S_contig17863.5435.1|Name=mRNA_Ecto-sp13_S_contig17863.5435.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=279bp|location=Sequence derived from alignment at Ecto-sp13_S_contig17863:3315..3595- (Ectocarpus species13 EcNAP12_S_4_19m)
GAAGAAGAGAATGCCAAGCAACACCAACAGCCGCTCAGCGGGTCAGAGGG
ACGACCACACCGACGACGACTGCGGCCGCTTGTTGCCGCCAGCCTTGGGT
GCTACGGAGCAGTGCTCGCCGACGGTTCGGAGTACCGAGGGGACTACGTC
GACACGCCGGCGGGAAGCCTGAAGGAGTTTCACGCCAGGCGGCTGGAGAT
CCTGGCGAGGGCGGACGGTGTCGACATGGTCGTGTTCGAGACGGTTCCTT
GCCTTGCGGAAGTGCGCGCCATCCTCTCG
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