mRNA_Ecto-sp13_S_contig17592.5308.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig17592.5308.1
Unique NamemRNA_Ecto-sp13_S_contig17592.5308.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig17592.5308.1 vs. uniprot
Match: A0A6H5KID0_9PHAE (Beta_elim_lyase domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KID0_9PHAE)

HSP 1 Score: 660 bits (1702), Expect = 7.950e-228
Identity = 439/475 (92.42%), Postives = 445/475 (93.68%), Query Frame = 1 
Query:    1 QVSGPDIQRTVSMMKEVLEEAGLNPLGVSRTPRYNAEDELEWHSPDDVEAAAXXXXXXXXXXDPTASPDQLGGTANGIVDGDEVVIVSEISNAVSGDAGPDSRGFPDSSFADAKTVVSPEMGSDGAATAELSTSAAAGXXXXXXXXAEAGLEAGGGADGLGEEGAGEKDKDELPPFVEKFEEVELQGMAVSTEGFVAIMTSKESTRALRLVVTPDDPMSGGLDVEQAETSEARTLLQLMQGIDVARHLPHDALGNLMDLSMRTGVQLSTVYVSHISPFSATLMAKVPQRDGETAAAEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-GVPETPVSPSVPGTTMGSGFGRPRLGLSKIAETTNAFEALGLALRYPSSRIFVRAELLAPTAEGGEAMGRKDSSSASSFESGDLAELYPNLMKLTDAKERRSASERMDSQFEMQKIRQQLESAV 1422
            QVSGPDIQRTVSMMKEVLEEAGLNPL VSRTPRYNAEDELEWHSP++VEAAA XXXXXXXXX   ASPDQLGGT NGIVDGDE VIVSEISNAVSGDAGPDSRGFPDSSF D KTVVSPEMGS  A TAELSTS AAG        AEAGLEAGGGADG+GEEGA + D DELPPFVEKFEEVELQGMAVSTEGFVAIMTSKESTRALRLVVTPDDPMSGGLDVEQAET EARTLLQLMQGIDVARHLPHDALGNLMDLSMRTGVQLSTVYVSHISPFSATLMAKVPQRDGETAAA XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX GVPETPVSPSVPGTTMGSGF RPRLGLSKIAETTN+FEALGLALRYPSSRIFVRAELLAP AEGGEAMGRKDSSSASSFES DLAELYPNLMKLTDAKERRSASERMDSQFEMQKIRQQLESA+
Sbjct:  427 QVSGPDIQRTVSMMKEVLEEAGLNPLVVSRTPRYNAEDELEWHSPENVEAAAAXXXXXXXXXXXAASPDQLGGTVNGIVDGDEAVIVSEISNAVSGDAGPDSRGFPDSSFTDVKTVVSPEMGSGSATTAELSTSTAAGAEGAGGGSAEAGLEAGGGADGVGEEGAEDDDNDELPPFVEKFEEVELQGMAVSTEGFVAIMTSKESTRALRLVVTPDDPMSGGLDVEQAETPEARTLLQLMQGIDVARHLPHDALGNLMDLSMRTGVQLSTVYVSHISPFSATLMAKVPQRDGETAAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGVPETPVSPSVPGTTMGSGFSRPRLGLSKIAETTNSFEALGLALRYPSSRIFVRAELLAPPAEGGEAMGRKDSSSASSFESRDLAELYPNLMKLTDAKERRSASERMDSQFEMQKIRQQLESAM 901
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BLAST of mRNA_Ecto-sp13_S_contig17592.5308.1 vs. uniprot
Match: K8Z7D4_NANGC (Threonine aldolase n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=K8Z7D4_NANGC)

HSP 1 Score: 84.7 bits (208), Expect = 3.210e-14
Identity = 53/138 (38.41%), Postives = 74/138 (53.62%), Query Frame = 1 
Query:  544 EVELQGMAVSTEGFVAIMTSKEST-----------RALRLVVTPDDPMSGGLDVEQAETSEARTLLQLMQGIDVARHLPHDALGNLMDLSMRTGVQLSTVYVSHISP------------FSATLMAKVPQRDGETAAA 888
            +V +QG++ S  GFV ++  KE             +AL++VVTP DPMS GLDV  AET EA TLLQL+Q IDV  +L HDAL +L+ +     V L  V +  +S             F A L  +V   +G +++A
Sbjct:   81 DVVVQGVSTSERGFVVVLAEKEGEGQEGSEGGRPRKALKVVVTPSDPMSAGLDVHAAETPEAITLLQLLQDIDVGSYLKHDALSSLVGVRAGHPVSLKRVVIEQLSAGRGVGGNNSNKRFKARLEVEVGDMEGASSSA 218
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BLAST of mRNA_Ecto-sp13_S_contig17592.5308.1 vs. uniprot
Match: A0A4D9CZ25_9STRA (Beta_elim_lyase domain-containing protein n=3 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9CZ25_9STRA)

HSP 1 Score: 85.9 bits (211), Expect = 5.350e-14
Identity = 58/159 (36.48%), Postives = 80/159 (50.31%), Query Frame = 1 
Query:  481 GEEGAGEKDKDELPPFVEKFEEVELQGMAVSTEGFVAIMTSKEST-----------RALRLVVTPDDPMSGGLDVEQAETSEARTLLQLMQGIDVARHLPHDALGNLMDLSMRTGVQLSTVYVSHISP------------FSATLMAKVPQRDGETAAA 888
            G  GAG      +    E   +V +QG++ S  GFV ++  KE             +AL++VVTP DPMS GLDV  AET EA TLLQL+Q IDV  +L HDAL +L+ +     V L  V +  +S             F A L  +V   +G +++A
Sbjct:  524 GPTGAGSAAAGPVSQAEETLVDVVVQGVSTSARGFVVVLAEKEGEGQEGSEGGSPRKALKVVVTPSDPMSAGLDVHAAETPEAITLLQLLQDIDVGSYLKHDALSSLVGVRAGHPVSLKRVVIEQLSAGRGAGGNNSNKRFKARLEVEVGDMEGASSSA 682
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BLAST of mRNA_Ecto-sp13_S_contig17592.5308.1 vs. uniprot
Match: A0A482SPV4_9ARCH (Aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SPV4_9ARCH)

HSP 1 Score: 72.0 bits (175), Expect = 1.200e-9
Identity = 47/121 (38.84%), Postives = 68/121 (56.20%), Query Frame = 1 
Query:  541 EEVELQGMAVSTEGFVAIMTSKESTRALRLVVTPDDPMSGGLDVEQAETSEARTLLQLMQGIDVARHLPHDALGNLMDLSMRTGVQLSTVYVSHISP----FSATLMAKVPQRDGETAAAE 891
            EE ++ GM+VS +GF   +      R +++ VTP DPM  GLD +QAE+SEA TLLQL+QGIDV   LP  AL +  +   +   +L  V V  +      F A L+A+  ++D    A +
Sbjct:  451 EETQVFGMSVSDQGFCVFLQGLICDRFVKVQVTPSDPMVYGLDRDQAESSEAVTLLQLLQGIDVESFLPKGALSSRFN-DGKLKYKLMRVVVDDLQDNGAGFRAKLIAQARKQDNSQEALD 570
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig17592.5308.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 4
Match NameE-valueIdentityDescription
A0A6H5KID0_9PHAE7.950e-22892.42Beta_elim_lyase domain-containing protein n=2 Tax=... [more]
K8Z7D4_NANGC3.210e-1438.41Threonine aldolase n=1 Tax=Nannochloropsis gaditan... [more]
A0A4D9CZ25_9STRA5.350e-1436.48Beta_elim_lyase domain-containing protein n=3 Tax=... [more]
A0A482SPV4_9ARCH1.200e-938.84Aminotransferase class I/II-fold pyridoxal phospha... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig17592contigEcto-sp13_S_contig17592:87..2845 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score674.9
Seed ortholog evalue2.9e-191
Seed eggNOG ortholog2880.D8LF44
Preferred nameTHA1
Model size1446
KEGG rclassRC00312,RC00372
KEGG koko:K01620
KEGG ReactionR00751,R06171
KEGG Pathwayko00260,ko01100,ko01110,ko01120,ko01130,ko01230,map00260,map01100,map01110,map01120,map01130,map01230
Hectar predicted targeting categoryother localisation
GOsGO:0001505,GO:0003674,GO:0003824,GO:0004793,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006082,GO:0006520,GO:0006544,GO:0006545,GO:0006566,GO:0006567,GO:0006807,GO:0008150,GO:0008152,GO:0008652,GO:0008732,GO:0009056,GO:0009058,GO:0009063,GO:0009066,GO:0009068,GO:0009069,GO:0009070,GO:0009987,GO:0016053,GO:0016054,GO:0016829,GO:0016830,GO:0016832,GO:0017144,GO:0019752,GO:0042133,GO:0042136,GO:0043436,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044281,GO:0044282,GO:0044283,GO:0044424,GO:0044444,GO:0044464,GO:0046394,GO:0046395,GO:0065007,GO:0065008,GO:0071704,GO:1901564,GO:1901565,GO:1901566,GO:1901575,GO:1901576,GO:1901605,GO:1901606,GO:1901607
Exons4
EggNOG free text desc.L-allo-threonine aldolase activity
EggNOG OGsCOG2008@1,KOG1368@2759
EC4.1.2.48
Cds size1446
COG Functional cat.E
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462914.586109-CDS-Ecto-sp13_S_contig17592:86..1971681462914.586109-CDS-Ecto-sp13_S_contig17592:86..197Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig17592 87..197 +
1681462914.5976968-CDS-Ecto-sp13_S_contig17592:509..9561681462914.5976968-CDS-Ecto-sp13_S_contig17592:509..956Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig17592 510..956 +
1681462914.6077652-CDS-Ecto-sp13_S_contig17592:1444..16061681462914.6077652-CDS-Ecto-sp13_S_contig17592:1444..1606Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig17592 1445..1606 +
1681462914.6178496-CDS-Ecto-sp13_S_contig17592:2119..28451681462914.6178496-CDS-Ecto-sp13_S_contig17592:2119..2845Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig17592 2120..2845 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig17592.5308.1prot_Ecto-sp13_S_contig17592.5308.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig17592 87..2845 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig17592.5308.1

>prot_Ecto-sp13_S_contig17592.5308.1 ID=prot_Ecto-sp13_S_contig17592.5308.1|Name=mRNA_Ecto-sp13_S_contig17592.5308.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=482bp
QVSGPDIQRTVSMMKEVLEEAGLNPLGVSRTPRYNAEDELEWHSPDDVEA
AAAAAAAAAAAADPTASPDQLGGTANGIVDGDEVVIVSEISNAVSGDAGP
DSRGFPDSSFADAKTVVSPEMGSDGAATAELSTSAAAGAEGATGVSAEAG
LEAGGGADGLGEEGAGEKDKDELPPFVEKFEEVELQGMAVSTEGFVAIMT
SKESTRALRLVVTPDDPMSGGLDVEQAETSEARTLLQLMQGIDVARHLPH
DALGNLMDLSMRTGVQLSTVYVSHISPFSATLMAKVPQRDGETAAAESAA
AAAQQLPSLPEDAAAAGTNADDAWFDGGAAATAAAAAAPPAPPTSADDGG
GVPETPVSPSVPGTTMGSGFGRPRLGLSKIAETTNAFEALGLALRYPSSR
IFVRAELLAPTAEGGEAMGRKDSSSASSFESGDLAELYPNLMKLTDAKER
RSASERMDSQFEMQKIRQQLESAVRVVVRCS*
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mRNA from alignment at Ecto-sp13_S_contig17592:87..2845+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig17592.5308.1 ID=mRNA_Ecto-sp13_S_contig17592.5308.1|Name=mRNA_Ecto-sp13_S_contig17592.5308.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2759bp|location=Sequence derived from alignment at Ecto-sp13_S_contig17592:87..2845+ (Ectocarpus species13 EcNAP12_S_4_19m)
CAGGTTTCCGGCCCGGACATCCAGCGGACGGTGTCAATGATGAAGGAAGT
GTTGGAGGAAGCCGGTCTTAACCCGCTGGGGGTAAGCCGCACGCCGCGCT
ACAACGCTGAGGTATTGATGCGCATCCATGCGCTCAGTCCCGCTTGTCCT
TGGAACGCAGTGCTTTCGTCGGTTAGGGTTTGATCTCCGCTCGGTTTTCG
GGATGGTTTGCGCGCGAAGAGCCACGCACGTGTGCGTACGCCGAGATGCT
TTTTTTGTCGGAAGATTGAGCTGTTCCCGGTACTGCGTTGCGTTCGGAAG
ATACATATTTGATGTTGCGGCGAATTGCTCGCATATCGCAGTCGCTATCA
ATCTCCACTGGATCAATATCCGTCCGTCTTGCCCCCACCACAAACACGCA
TACGTCTGTGCCGCAACCCCCAGGACGAGCTCGAGTGGCATTCACCTGAT
GACGTCGAAGCAGCAGCAGCAGCGGCGGCGGCCGCAGCGGCGGCGGCCGA
CCCCACCGCTTCTCCCGACCAGCTGGGAGGAACGGCCAACGGCATCGTGG
ACGGCGACGAGGTGGTCATCGTGAGCGAGATCTCCAACGCCGTCTCGGGA
GACGCAGGGCCGGACTCCCGCGGCTTCCCGGACTCTTCCTTCGCGGACGC
GAAGACGGTGGTATCTCCGGAGATGGGCTCTGACGGTGCTGCGACGGCGG
AATTGTCGACCTCAGCCGCCGCCGGTGCGGAAGGCGCTACGGGGGTTTCC
GCGGAGGCCGGTTTGGAGGCTGGCGGAGGCGCTGACGGCCTTGGAGAGGA
GGGTGCGGGGGAGAAGGATAAGGACGAGCTGCCGCCGTTCGTGGAGAAGT
TCGAGGAGGTTGAGCTCCAGGTACGTGGGCTGTCATTGCTGCTAATGTTG
TTGTTCTGTTGTTGTTGCTATTGCTGGTGGTTTGCTTTGTTTTTGGTCTA
TTTGGGGCCACGTTTTCCGGGCTTAGGACCCACCGACAGAGTCTGCCGTA
TGTTGGCTGTGGTGAGTACTTGCTCGCTCTCTTCGACCCCGGTATCAGAT
GTACGAAGAGCGGGAGAGAAAGAGAGATAGAGGACTGTAACAATGGCCAG
ATAGGTAGCCCTTCACCTGGATGTATTCGTACCAGAGAGACAGCCGTGAC
CTATGGCAGGTTGATTGTGCGTGCCATGGAGGGTATAAAACGCGCTCAAT
CTTGTGTGACCCTAGCTCGCCGATGTCTCAGTCCTAGCCTCGTACCAAAC
AGCTGCTACTCTGCTGGTATACATCCCCGCTTGCCTCCCCCCGGTTCTGT
CTCTTCCCGTGTGGTCCCGGGTGTTCCTGTCGCGTTTTTGGTCCCGCCAT
GCGCACAGGGCATGGCGGTTAGCACCGAAGGCTTCGTGGCGATAATGACA
AGCAAAGAGTCGACGCGAGCCCTCAGGCTGGTGGTAACTCCGGACGACCC
CATGTCGGGGGGTTTGGACGTCGAGCAGGCAGAGACTTCTGAGGCCCGCA
CCCTCCTACAGCTCATGCAGGTTTGTACGCCTCACCGAATTGTGCATTAG
TCCTTGTTGGTGCTTGCATGGTGGGACGGCCTTGGTGTCGCCGTGTTTGC
TCCCCCGTTTGTTTTGTCTTCCTGTCATGGCAGAGCTTTGTGGAGGTTGC
TCGCAAATCCGACCCACCTCCGTCGTACTGCATCGCCTGTATGCCCCCGA
TCATTTTTTCGGCGCGAAACAATGGAGAGAAATGTTGCTTCTTTGAGTGC
AAGGAACTGGGTACGAAATGAGGAAAAGAAGAAACACCACGCTTCGCCGA
GAATCGCACTCCCAACCAATTAATGTCGCTAGCTGTAGCTGTTTTCGTGC
TGTGTGGTGATGACCAAACTTCCCAGATGTGAAATTGGGGTCGATGGTGG
TAAAGGAGTCTACGCCTTGGTACCGTGCAGAGTCCCAAGCACAGCTATGA
TAGAGCAGCGCCCATTGTCACCACACCCACCCCAGCTGCCTATCGGTCTC
GTCTTGCGTTCCCCCTGCCGGCGTGCTTTCCAGGGCATCGACGTAGCCCG
CCACTTACCTCACGACGCCCTCGGCAACCTGATGGACCTCTCGATGAGGA
CCGGGGTCCAGCTGAGCACCGTGTACGTCAGCCACATCAGCCCCTTCTCG
GCCACCCTCATGGCGAAGGTTCCCCAGCGAGACGGCGAGACCGCAGCAGC
AGAATCGGCGGCGGCGGCGGCGCAGCAGCTTCCGTCCTTGCCGGAGGACG
CCGCTGCCGCCGGGACCAACGCCGACGACGCGTGGTTCGACGGAGGCGCG
GCTGCGACGGCGGCGGCGGCGGCGGCCCCGCCGGCACCGCCGACCTCTGC
GGATGACGGAGGAGGCGTACCGGAGACGCCAGTGTCGCCATCGGTTCCGG
GCACGACGATGGGCTCGGGCTTCGGCCGCCCGAGGCTCGGGCTGTCCAAG
ATAGCGGAGACGACGAATGCGTTTGAGGCCCTGGGACTTGCGCTGAGGTA
CCCGAGCAGCCGAATCTTCGTGCGGGCGGAGCTGCTGGCGCCGACGGCGG
AGGGGGGCGAGGCGATGGGGCGGAAGGACTCGTCCTCGGCGTCGAGCTTC
GAGTCGGGGGACCTCGCGGAGCTGTACCCGAACCTAATGAAGCTCACCGA
CGCCAAGGAGCGACGGTCGGCGTCCGAGAGGATGGACTCGCAGTTCGAGA
TGCAGAAGATTCGACAGCAGCTGGAGAGCGCGGTGCGTGTTGTTGTTCGT
TGTTCGTAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig17592:87..2845+

>mRNA_Ecto-sp13_S_contig17592.5308.1 ID=mRNA_Ecto-sp13_S_contig17592.5308.1|Name=mRNA_Ecto-sp13_S_contig17592.5308.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=1446bp|location=Sequence derived from alignment at Ecto-sp13_S_contig17592:87..2845+ (Ectocarpus species13 EcNAP12_S_4_19m)
CAGGTTTCCGGCCCGGACATCCAGCGGACGGTGTCAATGATGAAGGAAGT
GTTGGAGGAAGCCGGTCTTAACCCGCTGGGGGTAAGCCGCACGCCGCGCT
ACAACGCTGAGGACGAGCTCGAGTGGCATTCACCTGATGACGTCGAAGCA
GCAGCAGCAGCGGCGGCGGCCGCAGCGGCGGCGGCCGACCCCACCGCTTC
TCCCGACCAGCTGGGAGGAACGGCCAACGGCATCGTGGACGGCGACGAGG
TGGTCATCGTGAGCGAGATCTCCAACGCCGTCTCGGGAGACGCAGGGCCG
GACTCCCGCGGCTTCCCGGACTCTTCCTTCGCGGACGCGAAGACGGTGGT
ATCTCCGGAGATGGGCTCTGACGGTGCTGCGACGGCGGAATTGTCGACCT
CAGCCGCCGCCGGTGCGGAAGGCGCTACGGGGGTTTCCGCGGAGGCCGGT
TTGGAGGCTGGCGGAGGCGCTGACGGCCTTGGAGAGGAGGGTGCGGGGGA
GAAGGATAAGGACGAGCTGCCGCCGTTCGTGGAGAAGTTCGAGGAGGTTG
AGCTCCAGGGCATGGCGGTTAGCACCGAAGGCTTCGTGGCGATAATGACA
AGCAAAGAGTCGACGCGAGCCCTCAGGCTGGTGGTAACTCCGGACGACCC
CATGTCGGGGGGTTTGGACGTCGAGCAGGCAGAGACTTCTGAGGCCCGCA
CCCTCCTACAGCTCATGCAGGGCATCGACGTAGCCCGCCACTTACCTCAC
GACGCCCTCGGCAACCTGATGGACCTCTCGATGAGGACCGGGGTCCAGCT
GAGCACCGTGTACGTCAGCCACATCAGCCCCTTCTCGGCCACCCTCATGG
CGAAGGTTCCCCAGCGAGACGGCGAGACCGCAGCAGCAGAATCGGCGGCG
GCGGCGGCGCAGCAGCTTCCGTCCTTGCCGGAGGACGCCGCTGCCGCCGG
GACCAACGCCGACGACGCGTGGTTCGACGGAGGCGCGGCTGCGACGGCGG
CGGCGGCGGCGGCCCCGCCGGCACCGCCGACCTCTGCGGATGACGGAGGA
GGCGTACCGGAGACGCCAGTGTCGCCATCGGTTCCGGGCACGACGATGGG
CTCGGGCTTCGGCCGCCCGAGGCTCGGGCTGTCCAAGATAGCGGAGACGA
CGAATGCGTTTGAGGCCCTGGGACTTGCGCTGAGGTACCCGAGCAGCCGA
ATCTTCGTGCGGGCGGAGCTGCTGGCGCCGACGGCGGAGGGGGGCGAGGC
GATGGGGCGGAAGGACTCGTCCTCGGCGTCGAGCTTCGAGTCGGGGGACC
TCGCGGAGCTGTACCCGAACCTAATGAAGCTCACCGACGCCAAGGAGCGA
CGGTCGGCGTCCGAGAGGATGGACTCGCAGTTCGAGATGCAGAAGATTCG
ACAGCAGCTGGAGAGCGCGGTGCGTGTTGTTGTTCGTTGTTCGTAG
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