mRNA_Ecto-sp13_S_contig89056.20430.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig89056.20430.1
Unique NamemRNA_Ecto-sp13_S_contig89056.20430.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig89056.20430.1 vs. uniprot
Match: D7FUR8_ECTSI (Nitrate high affinity transporter n=4 Tax=Ectocarpus TaxID=2879 RepID=D7FUR8_ECTSI)

HSP 1 Score: 124 bits (310), Expect = 3.830e-32
Identity = 60/61 (98.36%), Postives = 61/61 (100.00%), Query Frame = 1 
Query:    1 CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAVIRFFIGVMGATFVCTQFWSSQIFAKE 183
            CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVA+IRFFIGVMGATFVCTQFWSSQIFAKE
Sbjct:   95 CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAIIRFFIGVMGATFVCTQFWSSQIFAKE 155
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BLAST of mRNA_Ecto-sp13_S_contig89056.20430.1 vs. uniprot
Match: D7FUS1_ECTSI (Nitrate/nitrite transporter n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FUS1_ECTSI)

HSP 1 Score: 116 bits (290), Expect = 2.820e-29
Identity = 54/61 (88.52%), Postives = 59/61 (96.72%), Query Frame = 1 
Query:    1 CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAVIRFFIGVMGATFVCTQFWSSQIFAKE 183
            CDTFGARI SSTLL+MGCIPT FAGLVNSATDVA+IRFFIGVMGATFVCTQ+W+SQ+FAKE
Sbjct:   95 CDTFGARITSSTLLLMGCIPTFFAGLVNSATDVAIIRFFIGVMGATFVCTQYWTSQVFAKE 155
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BLAST of mRNA_Ecto-sp13_S_contig89056.20430.1 vs. uniprot
Match: A0A7S2PGB4_9STRA (Hypothetical protein (Fragment) n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2PGB4_9STRA)

HSP 1 Score: 78.2 bits (191), Expect = 7.280e-16
Identity = 32/61 (52.46%), Postives = 48/61 (78.69%), Query Frame = 1 
Query:    1 CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAVIRFFIGVMGATFVCTQFWSSQIFAKE 183
            CD +G RI+ ST+L++ C+PTA  G+V SATD+ +IR FIG+ G++FV  Q+W+S++F KE
Sbjct:  103 CDKYGPRIMMSTILLIACVPTALTGVVESATDLLIIRMFIGIGGSSFVMCQYWTSRMFTKE 163
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BLAST of mRNA_Ecto-sp13_S_contig89056.20430.1 vs. uniprot
Match: A0A6U3Y6P5_9STRA (Nitrate/nitrite transporter n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A6U3Y6P5_9STRA)

HSP 1 Score: 78.6 bits (192), Expect = 1.440e-15
Identity = 35/61 (57.38%), Postives = 45/61 (73.77%), Query Frame = 1 
Query:    1 CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAVIRFFIGVMGATFVCTQFWSSQIFAKE 183
            CD +GARI+   LL+   IP A  GLVN A  +AV+RFFIG+ G+TFVC QFW+S++F KE
Sbjct:  108 CDKYGARILMGVLLMFASIPCALTGLVNDAASLAVLRFFIGLGGSTFVCCQFWTSRMFTKE 168
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BLAST of mRNA_Ecto-sp13_S_contig89056.20430.1 vs. uniprot
Match: A0A812P663_9DINO (NRT2.2 protein (Fragment) n=1 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812P663_9DINO)

HSP 1 Score: 77.0 bits (188), Expect = 2.740e-15
Identity = 38/61 (62.30%), Postives = 43/61 (70.49%), Query Frame = 1 
Query:    1 CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAVIRFFIGVMGATFVCTQFWSSQIFAKE 183
            CD FG R + S LL  G IP  F GLVN+AT +A IRFFIGV+GA FV  Q W SQ+FAKE
Sbjct:  131 CDVFGTRTMFSMLLSFGAIPVFFGGLVNNATGLATIRFFIGVLGACFVPCQAWCSQLFAKE 191
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BLAST of mRNA_Ecto-sp13_S_contig89056.20430.1 vs. uniprot
Match: K0TAW8_THAOC (Uncharacterized protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0TAW8_THAOC)

HSP 1 Score: 74.7 bits (182), Expect = 2.940e-15
Identity = 35/61 (57.38%), Postives = 45/61 (73.77%), Query Frame = 1 
Query:    1 CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAVIRFFIGVMGATFVCTQFWSSQIFAKE 183
            CD +GARI  S +L+   IP A  GLVNSAT +AV+RFFIG  G+TFV  Q+W+S++F KE
Sbjct:  100 CDKYGARIPMSIILVFASIPCACTGLVNSATSLAVLRFFIGFGGSTFVMCQYWTSRMFTKE 160
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BLAST of mRNA_Ecto-sp13_S_contig89056.20430.1 vs. uniprot
Match: A0A835YQD5_9STRA (Nitrate high affinity transporter n=2 Tax=Tribonema minus TaxID=303371 RepID=A0A835YQD5_9STRA)

HSP 1 Score: 76.6 bits (187), Expect = 6.850e-15
Identity = 36/61 (59.02%), Postives = 42/61 (68.85%), Query Frame = 1 
Query:    1 CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAVIRFFIGVMGATFVCTQFWSSQIFAKE 183
            CD FGARI+ + LL  G IPT F GLV  AT +AV RFF+G +GATFV  Q WSS +F K 
Sbjct:  104 CDAFGARIVMAGLLFFGAIPTFFQGLVQDATGLAVCRFFVGFLGATFVANQAWSSAMFGKN 164
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BLAST of mRNA_Ecto-sp13_S_contig89056.20430.1 vs. uniprot
Match: A0A812ST29_9DINO (Nitrate/nitrite transporter n=2 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812ST29_9DINO)

HSP 1 Score: 76.3 bits (186), Expect = 9.380e-15
Identity = 37/61 (60.66%), Postives = 43/61 (70.49%), Query Frame = 1 
Query:    1 CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAVIRFFIGVMGATFVCTQFWSSQIFAKE 183
            CD FG R + S LL  G IP  F GLVN+AT + +IRF IGV+GATFV  Q W SQ+FAKE
Sbjct:  119 CDRFGTRTMFSMLLTFGAIPVFFGGLVNNATGLVIIRFVIGVLGATFVPCQAWCSQLFAKE 179
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BLAST of mRNA_Ecto-sp13_S_contig89056.20430.1 vs. uniprot
Match: A0A835YTD5_9STRA (Nitrate high affinity transporter n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YTD5_9STRA)

HSP 1 Score: 74.7 bits (182), Expect = 3.280e-14
Identity = 35/61 (57.38%), Postives = 41/61 (67.21%), Query Frame = 1 
Query:    1 CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAVIRFFIGVMGATFVCTQFWSSQIFAKE 183
            CD FGARI+ + LL  G IPT F GLV  A  +AV RFF+G +GATFV  Q WSS +F K 
Sbjct:  102 CDAFGARIVMACLLFFGAIPTFFQGLVQDAQGLAVCRFFVGFLGATFVANQAWSSAMFGKS 162
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BLAST of mRNA_Ecto-sp13_S_contig89056.20430.1 vs. uniprot
Match: A0A812SSL6_9DINO (Nitrate/nitrite transporter n=3 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812SSL6_9DINO)

HSP 1 Score: 74.7 bits (182), Expect = 3.340e-14
Identity = 36/61 (59.02%), Postives = 42/61 (68.85%), Query Frame = 1 
Query:    1 CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAVIRFFIGVMGATFVCTQFWSSQIFAKE 183
            CD FG R + S LL  G IP  F GLVN+ T + +IRF IGV+GATFV  Q W SQ+FAKE
Sbjct:  182 CDRFGTRTMFSMLLTFGAIPVFFGGLVNNGTGLVIIRFVIGVLGATFVPCQAWCSQLFAKE 242
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig89056.20430.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FUR8_ECTSI3.830e-3298.36Nitrate high affinity transporter n=4 Tax=Ectocarp... [more]
D7FUS1_ECTSI2.820e-2988.52Nitrate/nitrite transporter n=2 Tax=Ectocarpus Tax... [more]
A0A7S2PGB4_9STRA7.280e-1652.46Hypothetical protein (Fragment) n=1 Tax=Leptocylin... [more]
A0A6U3Y6P5_9STRA1.440e-1557.38Nitrate/nitrite transporter n=1 Tax=Skeletonema ma... [more]
A0A812P663_9DINO2.740e-1562.30NRT2.2 protein (Fragment) n=1 Tax=Symbiodinium nat... [more]
K0TAW8_THAOC2.940e-1557.38Uncharacterized protein n=1 Tax=Thalassiosira ocea... [more]
A0A835YQD5_9STRA6.850e-1559.02Nitrate high affinity transporter n=2 Tax=Tribonem... [more]
A0A812ST29_9DINO9.380e-1560.66Nitrate/nitrite transporter n=2 Tax=Symbiodinium n... [more]
A0A835YTD5_9STRA3.280e-1457.38Nitrate high affinity transporter n=1 Tax=Tribonem... [more]
A0A812SSL6_9DINO3.340e-1459.02Nitrate/nitrite transporter n=3 Tax=Symbiodinium n... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig89056contigEcto-sp13_S_contig89056:23..205 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score122.5
Seed ortholog evalue6.9e-26
Seed eggNOG ortholog2880.D7FUR8
Preferred nameNRT2.2
Model size183
KEGG koko:K02575
KEGG TC2.A.1.8
KEGG Pathwayko00910,map00910
KEGG ModuleM00615
Hectar predicted targeting categorysignal peptide
GOsGO:0000325,GO:0001101,GO:0003674,GO:0005215,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005773,GO:0005774,GO:0005886,GO:0006810,GO:0006811,GO:0006820,GO:0007275,GO:0008150,GO:0008509,GO:0009507,GO:0009536,GO:0009628,GO:0009705,GO:0009791,GO:0009987,GO:0010035,GO:0010167,GO:0015075,GO:0015103,GO:0015112,GO:0015267,GO:0015291,GO:0015318,GO:0015513,GO:0015698,GO:0015706,GO:0015707,GO:0016020,GO:0022622,GO:0022803,GO:0022804,GO:0022832,GO:0022836,GO:0022857,GO:0031090,GO:0032501,GO:0032502,GO:0032991,GO:0034220,GO:0042221,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0048364,GO:0048527,GO:0048528,GO:0048731,GO:0048856,GO:0050896,GO:0051179,GO:0051234,GO:0051716,GO:0055085,GO:0070887,GO:0071229,GO:0071241,GO:0071249,GO:0071705,GO:0071944,GO:0080167,GO:0090696,GO:0098588,GO:0098656,GO:0098805,GO:0099402,GO:1901698,GO:1901699,GO:1901700,GO:1901701,GO:1902170,GO:1990351
Exons1
EggNOG free text desc.cellular response to nitrate
EggNOG OGs2QPIC@2759,COG2223@1
Cds size183
COG Functional cat.P
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko02000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464163.88291-CDS-Ecto-sp13_S_contig89056:22..2051681464163.88291-CDS-Ecto-sp13_S_contig89056:22..205Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig89056 23..205 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig89056.20430.1prot_Ecto-sp13_S_contig89056.20430.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig89056 23..205 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig89056.20430.1

>prot_Ecto-sp13_S_contig89056.20430.1 ID=prot_Ecto-sp13_S_contig89056.20430.1|Name=mRNA_Ecto-sp13_S_contig89056.20430.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=61bp
CDTFGARIISSTLLIMGCIPTAFAGLVNSATDVAVIRFFIGVMGATFVCT
QFWSSQIFAKE
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mRNA from alignment at Ecto-sp13_S_contig89056:23..205+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig89056.20430.1 ID=mRNA_Ecto-sp13_S_contig89056.20430.1|Name=mRNA_Ecto-sp13_S_contig89056.20430.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=183bp|location=Sequence derived from alignment at Ecto-sp13_S_contig89056:23..205+ (Ectocarpus species13 EcNAP12_S_4_19m)
TGCGACACCTTCGGCGCGCGCATCATCTCCAGCACCCTGCTCATCATGGG
GTGTATCCCGACCGCCTTCGCCGGCCTGGTGAACAGCGCCACCGACGTGG
CCGTCATCCGCTTCTTCATCGGCGTGATGGGCGCCACGTTCGTGTGCACG
CAGTTCTGGTCCTCGCAGATCTTCGCGAAGGAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig89056:23..205+

>mRNA_Ecto-sp13_S_contig89056.20430.1 ID=mRNA_Ecto-sp13_S_contig89056.20430.1|Name=mRNA_Ecto-sp13_S_contig89056.20430.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=183bp|location=Sequence derived from alignment at Ecto-sp13_S_contig89056:23..205+ (Ectocarpus species13 EcNAP12_S_4_19m)
TGCGACACCTTCGGCGCGCGCATCATCTCCAGCACCCTGCTCATCATGGG
GTGTATCCCGACCGCCTTCGCCGGCCTGGTGAACAGCGCCACCGACGTGG
CCGTCATCCGCTTCTTCATCGGCGTGATGGGCGCCACGTTCGTGTGCACG
CAGTTCTGGTCCTCGCAGATCTTCGCGAAGGAG
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