mRNA_Ecto-sp13_S_contig6417.17179.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig6417.17179.1
Unique NamemRNA_Ecto-sp13_S_contig6417.17179.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig6417.17179.1 vs. uniprot
Match: D8LEV0_ECTSI (Peptidyl-prolyl cis-trans isomerase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LEV0_ECTSI)

HSP 1 Score: 349 bits (896), Expect = 1.760e-121
Identity = 181/184 (98.37%), Postives = 183/184 (99.46%), Query Frame = 1 
Query:    1 MMRKTVLLLLCVLVSVAGFVPAPSICRNARSAVGTPARTSTSSLSMGFDFKGTLEKMSDTRYARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYVESCNKPQNTWKMLFDDIVDKVSGKEEEGDKK 552
            MMR+T+LLLLCVLVSVAGFVPAPSICRNARSAVGTPARTSTSSLSMGFDFKGTLEKMSD RYARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYVESCNKPQNTWKMLFDDIVDKVSGKEEEGDKK
Sbjct:    1 MMRETILLLLCVLVSVAGFVPAPSICRNARSAVGTPARTSTSSLSMGFDFKGTLEKMSDPRYARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYVESCNKPQNTWKMLFDDIVDKVSGKEEEGDKK 184
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BLAST of mRNA_Ecto-sp13_S_contig6417.17179.1 vs. uniprot
Match: A0A6U8PGI0_EMIHU (Peptidyl-prolyl cis-trans isomerase n=2 Tax=Emiliania huxleyi TaxID=2903 RepID=A0A6U8PGI0_EMIHU)

HSP 1 Score: 97.8 bits (242), Expect = 1.830e-22
Identity = 49/109 (44.95%), Postives = 65/109 (59.63%), Query Frame = 1 
Query:  160 LEKMSDTRYARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYVESCNKPQNT 486
            +++M+D R  R  HI++    +     L E K EI  D  KF E A   S C SAPRGG L  + RG+  PEFD++ F  E G V+GP++TQFG HLIY+ SC +P  T
Sbjct:   46 MKQMTDQRVGRASHIMLRPSDDAAAELLREWKAEIGDDEAKFQERARASSECRSAPRGGDLGFVTRGKLSPEFDEVIFEEEPGFVYGPLQTQFGYHLIYLHSCREPSGT 154
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BLAST of mRNA_Ecto-sp13_S_contig6417.17179.1 vs. uniprot
Match: A0A7S1Y1S1_9STRA (Peptidyl-prolyl cis-trans isomerase n=1 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1Y1S1_9STRA)

HSP 1 Score: 89.0 bits (219), Expect = 2.520e-19
Identity = 55/116 (47.41%), Postives = 72/116 (62.07%), Query Frame = 1 
Query:  115 TSTSSLSMGF-DFKGTLEKMSDTRYARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYV 459
            ++T+ L  GF D  GT  K   T+ A   HIL++  G E  +KLEE K EI     KF+E A + S+C SA +GG L     G+ V EFD + FN ++GVVHGP++TQFG HLIYV
Sbjct:   23 STTTKLFGGFMDEVGTFFKRF-TQKASASHILIKG-GPEAANKLEELKVEIDNSPVKFAEAASRFSSCPSARKGGELGEFGPGQMVKEFDQVVFNDDVGVVHGPIKTQFGYHLIYV 136
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BLAST of mRNA_Ecto-sp13_S_contig6417.17179.1 vs. uniprot
Match: A0A7S2HC75_9STRA (Peptidyl-prolyl cis-trans isomerase n=1 Tax=Helicotheca tamesis TaxID=374047 RepID=A0A7S2HC75_9STRA)

HSP 1 Score: 87.0 bits (214), Expect = 7.040e-19
Identity = 43/92 (46.74%), Postives = 56/92 (60.87%), Query Frame = 1 
Query:  187 ARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYVE 462
            A   HIL++    E   K++  KEEI  D+ KF+E A + S C S  +GG L    R   VPEFD + FN E+GVVHGP+ T FG HLI++E
Sbjct:   18 AEASHILIKGTDAEAEQKIKAIKEEIGNDVTKFAEAATQHSDCPSGRQGGSLGEFGRYSMVPEFDKVVFNEEVGVVHGPIRTSFGFHLIFIE 109
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BLAST of mRNA_Ecto-sp13_S_contig6417.17179.1 vs. uniprot
Match: A0A2E3DHP9_9PLAN (Uncharacterized protein n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A2E3DHP9_9PLAN)

HSP 1 Score: 87.4 bits (215), Expect = 2.960e-18
Identity = 50/95 (52.63%), Postives = 59/95 (62.11%), Query Frame = 1 
Query:  187 ARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYVESCN 471
            A  RHILVE K        EE K +IAG LD F+  A + S C S+ +GG L    RG+ VPEFD + F  E+GVVHGPVETQFG HLI + S N
Sbjct:   97 AAARHILVETK-----EACEELKSKIAGGLD-FAAAAAEHSKCPSSSQGGELGTFGRGQMVPEFDKVVFEEEVGVVHGPVETQFGFHLIEITSRN 185
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BLAST of mRNA_Ecto-sp13_S_contig6417.17179.1 vs. uniprot
Match: A0A7R9W6N6_9STRA (Peptidyl-prolyl cis-trans isomerase n=1 Tax=Pseudictyota dubia TaxID=2749911 RepID=A0A7R9W6N6_9STRA)

HSP 1 Score: 86.7 bits (213), Expect = 3.850e-18
Identity = 44/98 (44.90%), Postives = 58/98 (59.18%), Query Frame = 1 
Query:  178 TRYARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYVESCN 471
            T  A   HIL++      + K+++ K+EI  D DKF+E+A   S C S  +GG L    R   VPEFD + FN E+GVVHGP+ET FG HLI +   N
Sbjct:   71 TMRAEASHILIKGTDSGAKRKIDDIKKEIGNDSDKFAELAALTSDCPSGQQGGSLGEFGRYSMVPEFDKVVFNEEVGVVHGPIETSFGYHLILIHRRN 168
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BLAST of mRNA_Ecto-sp13_S_contig6417.17179.1 vs. uniprot
Match: A0A2D8J063_9ALTE (Peptidylprolyl isomerase n=2 Tax=Alteromonadaceae TaxID=72275 RepID=A0A2D8J063_9ALTE)

HSP 1 Score: 83.6 bits (205), Expect = 8.590e-18
Identity = 46/93 (49.46%), Postives = 59/93 (63.44%), Query Frame = 1 
Query:  187 ARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYVES 465
            A  RHILV++K +      E  K+EI G  D F+ VA + STC S  +GG L    RG+ VPEFD + F+G+L  VHGPV+TQFG HL+ V S
Sbjct:    4 ASARHILVKDKAQ-----CENLKKEIEGGRD-FAAVAREHSTCPSGKQGGDLGEFGRGQMVPEFDQVVFSGDLNTVHGPVQTQFGYHLLEVTS 90
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BLAST of mRNA_Ecto-sp13_S_contig6417.17179.1 vs. uniprot
Match: S0JGD6_9FIRM (Peptidylprolyl isomerase n=1 Tax=Eubacterium sp. 14-2 TaxID=1235790 RepID=S0JGD6_9FIRM)

HSP 1 Score: 87.4 bits (215), Expect = 1.110e-17
Identity = 52/121 (42.98%), Postives = 72/121 (59.50%), Query Frame = 1 
Query:  160 LEKMSDTRYARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYVESCNKPQNTWKMLFDDIVDKV 522
            +E+  +    R RHILV+      R + E   +E+   LD F+E AEK STC S  +GG L    RG+ VPEF+  AF GE+G + GPVETQFG HLI VE  +K + + K  FD + +++
Sbjct:  107 MEQFQEKAQVRARHILVDT-----RQQAESVSQELEQGLD-FAEAAEKYSTCPSREKGGDLGYFTRGQMVPEFEKAAFEGEIGKLIGPVETQFGCHLILVE--DKKEGSVKP-FDQVQEQI 218
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BLAST of mRNA_Ecto-sp13_S_contig6417.17179.1 vs. uniprot
Match: UPI0012644084 (peptidylprolyl isomerase n=1 Tax=Sulfurimonas indica TaxID=2508707 RepID=UPI0012644084)

HSP 1 Score: 83.2 bits (204), Expect = 1.180e-17
Identity = 45/92 (48.91%), Postives = 59/92 (64.13%), Query Frame = 1 
Query:  184 YARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYV 459
            +A  RHILVE +     +K  E KEEI    D F E+A++ STC S+  GG L     G+ VPEFD   FNG++GVV+GP++TQFG HL+ V
Sbjct:    3 WATARHILVESE-----AKCNELKEEIENGAD-FGEIAKQYSTCPSSRNGGDLGQFGPGQMVPEFDKAVFNGDVGVVYGPIQTQFGYHLLEV 88
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BLAST of mRNA_Ecto-sp13_S_contig6417.17179.1 vs. uniprot
Match: A0A1W1BRN3_9ZZZZ (Peptidyl-prolyl cis-trans isomerase PpiC n=1 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A1W1BRN3_9ZZZZ)

HSP 1 Score: 83.2 bits (204), Expect = 1.210e-17
Identity = 43/91 (47.25%), Postives = 57/91 (62.64%), Query Frame = 1 
Query:  187 ARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAEKMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHLIYV 459
            A  RHILV ++     SK  + KE+I      F EVA   S C S  +GG L + ++G+ VPEFD + FN E+GVVHGP++TQFG HLI +
Sbjct:    4 ASARHILVNDE-----SKCMDLKEQIVSGQKTFVEVARDQSLCPSGVKGGDLGIFSQGQMVPEFDKVVFNDEVGVVHGPIQTQFGYHLIEI 89
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig6417.17179.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LEV0_ECTSI1.760e-12198.37Peptidyl-prolyl cis-trans isomerase n=2 Tax=Ectoca... [more]
A0A6U8PGI0_EMIHU1.830e-2244.95Peptidyl-prolyl cis-trans isomerase n=2 Tax=Emilia... [more]
A0A7S1Y1S1_9STRA2.520e-1947.41Peptidyl-prolyl cis-trans isomerase n=1 Tax=Gramma... [more]
A0A7S2HC75_9STRA7.040e-1946.74Peptidyl-prolyl cis-trans isomerase n=1 Tax=Helico... [more]
A0A2E3DHP9_9PLAN2.960e-1852.63Uncharacterized protein n=1 Tax=Planctomycetaceae ... [more]
A0A7R9W6N6_9STRA3.850e-1844.90Peptidyl-prolyl cis-trans isomerase n=1 Tax=Pseudi... [more]
A0A2D8J063_9ALTE8.590e-1849.46Peptidylprolyl isomerase n=2 Tax=Alteromonadaceae ... [more]
S0JGD6_9FIRM1.110e-1742.98Peptidylprolyl isomerase n=1 Tax=Eubacterium sp. 1... [more]
UPI00126440841.180e-1748.91peptidylprolyl isomerase n=1 Tax=Sulfurimonas indi... [more]
A0A1W1BRN3_9ZZZZ1.210e-1747.25Peptidyl-prolyl cis-trans isomerase PpiC n=1 Tax=h... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig6417contigEcto-sp13_S_contig6417:530..2915 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score352.4
Seed ortholog evalue1.2e-94
Seed eggNOG ortholog2880.D8LEV0
Model size555
Hectar predicted targeting categorysignal peptide
Exons4
EggNOG free text desc.PPIC-type PPIASE domain
EggNOG OGs2SBZU@2759,COG0760@1
Ec32 ortholog descriptionPeptidyl-prolyl cis-trans isomerase, PpiC-type
Ec32 orthologEc-21_005670.1
Cds size555
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463887.2557626-CDS-Ecto-sp13_S_contig6417:529..6381681463887.2557626-CDS-Ecto-sp13_S_contig6417:529..638Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig6417 530..638 +
1681463887.272062-CDS-Ecto-sp13_S_contig6417:1562..17771681463887.272062-CDS-Ecto-sp13_S_contig6417:1562..1777Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig6417 1563..1777 +
1681463887.280939-CDS-Ecto-sp13_S_contig6417:2250..23441681463887.280939-CDS-Ecto-sp13_S_contig6417:2250..2344Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig6417 2251..2344 +
1681463887.2902844-CDS-Ecto-sp13_S_contig6417:2778..29151681463887.2902844-CDS-Ecto-sp13_S_contig6417:2778..2915Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig6417 2779..2915 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig6417.17179.1prot_Ecto-sp13_S_contig6417.17179.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig6417 530..2915 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig6417.17179.1

>prot_Ecto-sp13_S_contig6417.17179.1 ID=prot_Ecto-sp13_S_contig6417.17179.1|Name=mRNA_Ecto-sp13_S_contig6417.17179.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=185bp
MMRKTVLLLLCVLVSVAGFVPAPSICRNARSAVGTPARTSTSSLSMGFDF
KGTLEKMSDTRYARVRHILVEEKGEEGRSKLEEAKEEIAGDLDKFSEVAE
KMSTCTSAPRGGRLKLLARGETVPEFDDIAFNGELGVVHGPVETQFGTHL
IYVESCNKPQNTWKMLFDDIVDKVSGKEEEGDKK*
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mRNA from alignment at Ecto-sp13_S_contig6417:530..2915+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig6417.17179.1 ID=mRNA_Ecto-sp13_S_contig6417.17179.1|Name=mRNA_Ecto-sp13_S_contig6417.17179.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2386bp|location=Sequence derived from alignment at Ecto-sp13_S_contig6417:530..2915+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGATGCGGAAAACGGTACTGCTTCTGTTGTGCGTGCTGGTGAGCGTGGC
TGGGTTCGTCCCCGCGCCTTCTATCTGCCGGAATGCGCGCTCGGCTGTGG
GCACACCAGGTAAGGTCACCACTGCCTTGTTGATGCATCGTTCTGCGGCT
GCTGCTATTACATGCTGCTTAGTGTGCAGGGCGGCGAGTTAGTTCTGGCG
CGATTACTGCTCTTCCCGGAAATAGTAACCGCTAAATCTAGCCGTCATGG
TGCTGCTGCGATGGTGGTGTTGATTTGTGGTATGATGTCGACAAGGATCA
CGAGCAGAGCGAGGAGCAAATTCCTGCTCCCGACTGCAGACATGTTCTTT
AACCAAGAGACATGTGTTTTTAATTTGTCGCACTACAGCAGCTGGCATCC
TAGCTTTTCAAGCTCCCCACCGACAGGCACTCACAGCCGTAGACCTTGTG
GTGATGTGCCCTGCCTTGGTCGTTTGGCTGAATATGATTGATGTTTTCTT
GACAATATAGTATGACAGCACCTACCGGCGAGCGCCAACTACTTGCTGTG
CCCTGACCCAACGACCACAACTCGCAGCAGTACAGACGTGGACTACTGTA
TGCATTGAGACGGAATCTGTAAGTTATCATTTGTCCGAGCGTTACTAAGA
CGTTACATTTGATCGAGGCAGCTTGGAAACTCTACAACACTAGGATTACT
AGCTTGAAACTGCGAGGCATCGATATTGCACCGGTGTAGAAGGGTCGTAT
CGGGATGGGGTGGGGCGATATGACCAATGTTTCCTGAGCATTGTCCCACC
GGATATGACCACTGTCTCTACCGCAGGTCGATTCCAACGCTATCGCGAGA
TGTTGTGAAAGAAAACCGCATGACACCTACTGCCAGTGTAAATGATGACG
TAGATACCTTTTTCCCGGACATTTCCCCCCGAGGACTTGATTCCTCACGC
CATATCATGTTGACCCTATACTAATTATTTGCGCACGAATCGTGTATTCT
CGAAACATGCGTTCGTTGCAATCAAACTTGTAGCAAGGACATCAACGTCG
TCGTTGTCCATGGGCTTCGACTTCAAGGGGACGCTGGAGAAGATGTCGGA
CACCCGCTACGCGCGGGTGAGGCACATCTTGGTCGAGGAGAAGGGAGAGG
AGGGTCGCTCCAAGCTGGAGGAGGCCAAGGAAGAGATCGCGGGAGACCTC
GACAAGTTCTCCGAAGTGGCGGAGAAGATGAGCACGTGTACCAGTGCGGT
GAGCCGAGCATTTTTTTCTACTGCTGGAAGGTGTTGGATCTTCTAGGGTG
CTGGGTCATGGAACCACCGCACGCACCCCGCCAAATTGAAGGTGGAGCGC
GGTGTTACGTGGGCCCAGCTGAACCGCACCGCAAATGCAGCATCTAGGAC
CCCACGACGGAGGGATCCGTTGCGATATCCACACATTTTTGTGGAGGTGG
CCTTAACTTCCTTGACTTGGGTCACATCTCCCCAACATCCGTGGTGGACG
ATTCCCTTTTGTCACATGGGCGTCGACTATGAAATGTTGGAAGATAAGCC
GGGATCAAATAGCGCTACCGTAGCCGCCAAACACTAAGAAATCATGGCAA
CCGCAAAATAACGATCGAGGGCCGATCAGAGTGTCCAACGTCTGTCGAGG
AAGGATACCTATATCTTTTTCACAGGTGCGTGCGGCTGCTCGCAATGTTC
TGTCTGTCATGGTGTGTGCAGCCCAGGGGGGGTAGACTGAAGCTGCTGGC
TCGTGGGGAGACGGTTCCCGAATTTGACGACATCGCGTTCAACGGGGAGC
TCGGAGTGGTCCACGGTGGGTCTTCCTCTTTCCGTTTTCCTGGGGAGATC
ATCGAAATATTGAGTCTCGTCGTTGCATTCATTTGTGGCGATAGTGTTGG
TGGCGGGTATGGTGGCGCTCTTGTCCTGCCCGCGAATCGGTTGACCTGTT
TTTCGTCCATCTACAACACGACATGAGTGCGATCTGATTCTGCCTTGAGG
GAGAAACACTCAACATACGTCAACAGACAACACACGCATTGAGAGATTCC
GCACCCCACGTAAGCGTTGTACCGGCCCAGCTGTCGCATGTATTCTCCGC
GTAGGTTTGGTTTGACGCCGAATGATGTAGCTACCAGGCAATTTGTTGAG
GTCCTGCTGCAGTGGGACCCGAAGCCTTAGCGGAGACTCGACTTCCAATA
CACCTCTATTTGTGCCTTTTTTGATGTTGTCCTCTGTATGTGATTTCAGG
GCCGGTAGAGACGCAATTCGGGACCCACCTTATCTACGTGGAGAGCTGCA
ACAAGCCGCAGAACACGTGGAAAATGCTCTTCGACGACATCGTCGACAAG
GTCTCGGGGAAGGAGGAAGAGGGGGATAAGAAGTAA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig6417:530..2915+

>mRNA_Ecto-sp13_S_contig6417.17179.1 ID=mRNA_Ecto-sp13_S_contig6417.17179.1|Name=mRNA_Ecto-sp13_S_contig6417.17179.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=555bp|location=Sequence derived from alignment at Ecto-sp13_S_contig6417:530..2915+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGATGCGGAAAACGGTACTGCTTCTGTTGTGCGTGCTGGTGAGCGTGGC
TGGGTTCGTCCCCGCGCCTTCTATCTGCCGGAATGCGCGCTCGGCTGTGG
GCACACCAGCAAGGACATCAACGTCGTCGTTGTCCATGGGCTTCGACTTC
AAGGGGACGCTGGAGAAGATGTCGGACACCCGCTACGCGCGGGTGAGGCA
CATCTTGGTCGAGGAGAAGGGAGAGGAGGGTCGCTCCAAGCTGGAGGAGG
CCAAGGAAGAGATCGCGGGAGACCTCGACAAGTTCTCCGAAGTGGCGGAG
AAGATGAGCACGTGTACCAGTGCGCCCAGGGGGGGTAGACTGAAGCTGCT
GGCTCGTGGGGAGACGGTTCCCGAATTTGACGACATCGCGTTCAACGGGG
AGCTCGGAGTGGTCCACGGGCCGGTAGAGACGCAATTCGGGACCCACCTT
ATCTACGTGGAGAGCTGCAACAAGCCGCAGAACACGTGGAAAATGCTCTT
CGACGACATCGTCGACAAGGTCTCGGGGAAGGAGGAAGAGGGGGATAAGA
AGTAA
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