mRNA_Ecto-sp13_S_contig20028.6534.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig20028.6534.1
Unique NamemRNA_Ecto-sp13_S_contig20028.6534.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig20028.6534.1 vs. uniprot
Match: D8LE56_ECTSI (TFIIS-type domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LE56_ECTSI)

HSP 1 Score: 180 bits (456), Expect = 2.540e-55
Identity = 84/87 (96.55%), Postives = 84/87 (96.55%), Query Frame = 1 
Query:   16 CLRSNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQADE 276
            C   NNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQADE
Sbjct:  127 CSECNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQADE 213
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BLAST of mRNA_Ecto-sp13_S_contig20028.6534.1 vs. uniprot
Match: A0A835YYP5_9STRA (DNA-directed RNA polymerase subunit n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YYP5_9STRA)

HSP 1 Score: 124 bits (312), Expect = 4.320e-35
Identity = 57/87 (65.52%), Postives = 68/87 (78.16%), Query Frame = 1 
Query:   16 CLRSNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQADE 276
            C   NNI+HPKEN+T R L Y+CNRC Y E+A   SCVY NNL+ T   KLDIV  DVV+DPTLQRSK+ IC++CNY+EAVFFQAD+
Sbjct:    4 CKECNNILHPKENRTTRTLFYACNRCAYREEAGQ-SCVYENNLVKTTELKLDIVHEDVVNDPTLQRSKHQICDRCNYSEAVFFQADD 89
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BLAST of mRNA_Ecto-sp13_S_contig20028.6534.1 vs. uniprot
Match: A0A8H7VS23_9FUNG (DNA-directed RNA polymerase subunit n=1 Tax=Mucor circinatus TaxID=2054153 RepID=A0A8H7VS23_9FUNG)

HSP 1 Score: 91.7 bits (226), Expect = 5.580e-22
Identity = 45/92 (48.91%), Postives = 64/92 (69.57%), Query Frame = 1 
Query:    7 FSLCLRSNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQADEVR 282
            F  CL  NN+++P+E+KT+R+L+++C  C+YEEDA +  CVYR+ +I  A  +  IV +D+  DPTL R+ N ICEKC   EAVFFQ+   R
Sbjct:    4 FKYCLDCNNLLYPREDKTNRRLLFACRNCQYEEDADN-LCVYRHEIIHAASEQTMIV-SDLSADPTLPRA-NVICEKCGSQEAVFFQSTSRR 92
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BLAST of mRNA_Ecto-sp13_S_contig20028.6534.1 vs. uniprot
Match: K0THW0_THAOC (TFIIS-type domain-containing protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0THW0_THAOC)

HSP 1 Score: 92.0 bits (227), Expect = 1.980e-21
Identity = 42/91 (46.15%), Postives = 62/91 (68.13%), Query Frame = 1 
Query:    1 IVFSLCLRSNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQAD 273
            ++   C   +++++PKEN ++RKL+Y+C  C+Y E A +G  +Y+N L    GN L  V  DV DDPTL RS+NA C KC ++EAV+FQ+D
Sbjct:   63 LLMRFCPHDSSMLYPKENPSERKLVYACRLCRYTEYA-AGQLIYKNVLKKEVGNILHTVPADVSDDPTLPRSQNAHCGKCGHSEAVYFQSD 152
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BLAST of mRNA_Ecto-sp13_S_contig20028.6534.1 vs. uniprot
Match: A0A162XV76_PHYB8 (DNA-directed RNA polymerase subunit n=1 Tax=Phycomyces blakesleeanus (strain ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC 33097 / NRRL 1555) TaxID=763407 RepID=A0A162XV76_PHYB8)

HSP 1 Score: 89.4 bits (220), Expect = 4.540e-21
Identity = 42/92 (45.65%), Postives = 62/92 (67.39%), Query Frame = 1 
Query:    7 FSLCLRSNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQADEVR 282
            F  CL  NN+++P+E+K+ R+LM+SC  C+YEEDA +  CVY + +I T   +  ++ TD+  DPTL R+ N  C +CN+ EAVFFQ+   R
Sbjct:    4 FKYCLECNNLLYPREDKSSRRLMFSCRNCQYEEDADN-MCVYSHKIIHTPSEQT-MMLTDLATDPTLPRA-NVTCPQCNHPEAVFFQSSSRR 92
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BLAST of mRNA_Ecto-sp13_S_contig20028.6534.1 vs. uniprot
Match: A0A7S3NJ32_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3NJ32_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 9.820e-21
Identity = 40/86 (46.51%), Postives = 54/86 (62.79%), Query Frame = 1 
Query:   16 CLRSNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQAD 273
            C +  N+  P+E +T R LM +C  C YEE A S   VY N +      +LDI+  ++ DDPTLQRS + +CE CNY+EAV FQA+
Sbjct:   36 CPQCGNMWQPREERTTRTLMLNCRNCGYEEPATSYK-VYENRIKKEVSTRLDIINPEITDDPTLQRSNDVVCENCNYSEAVLFQAE 120
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BLAST of mRNA_Ecto-sp13_S_contig20028.6534.1 vs. uniprot
Match: A0A8B7CCB1_PHODC (DNA-directed RNA polymerase subunit n=3 Tax=Arecaceae TaxID=4710 RepID=A0A8B7CCB1_PHODC)

HSP 1 Score: 86.3 bits (212), Expect = 7.410e-20
Identity = 42/88 (47.73%), Postives = 57/88 (64.77%), Query Frame = 1 
Query:    7 FSLCLRSNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQA 270
               C   NNI++PKE+K  + L+++C  C YEE A S  CVYRN +    G +  I+Q DV +DPTL R+K+  C KC++ EAVFFQA
Sbjct:    4 MKFCRECNNILYPKEDKQKKILLFACRNCDYEEVADS-FCVYRNEVHHAVGERTQILQ-DVANDPTLPRTKDVRCVKCDHPEAVFFQA 89
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BLAST of mRNA_Ecto-sp13_S_contig20028.6534.1 vs. uniprot
Match: A0A1X2HED9_SYNRA (DNA-directed RNA polymerase subunit n=1 Tax=Syncephalastrum racemosum TaxID=13706 RepID=A0A1X2HED9_SYNRA)

HSP 1 Score: 85.5 bits (210), Expect = 1.490e-19
Identity = 41/92 (44.57%), Postives = 61/92 (66.30%), Query Frame = 1 
Query:    7 FSLCLRSNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQADEVR 282
            F  CL  NN+++P+E+K +R+LM++C  C+YEEDA +  CVYR+ +I     +  +V +D+  DPTL R+ N  C +C Y EAVFFQ+   R
Sbjct:    4 FKYCLDCNNLLYPREDKANRRLMFACRNCQYEEDADN-FCVYRHEIIHQPSEQT-MVLSDLSIDPTLPRA-NVTCPQCGYEEAVFFQSSSRR 92
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BLAST of mRNA_Ecto-sp13_S_contig20028.6534.1 vs. uniprot
Match: A0A8H7UCI9_9FUNG (DNA-directed RNA polymerase subunit n=2 Tax=Umbelopsis TaxID=64561 RepID=A0A8H7UCI9_9FUNG)

HSP 1 Score: 85.5 bits (210), Expect = 1.490e-19
Identity = 40/88 (45.45%), Postives = 62/88 (70.45%), Query Frame = 1 
Query:    7 FSLCLRSNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQA 270
            F  C   NN+++P+E+KT+RKL+++C  C +EEDA + +CVYR+ ++  A ++  +V TD+  DPTL R+  A C +C Y EAVFFQ+
Sbjct:    4 FKYCSECNNMLYPREDKTNRKLLFACRNCHFEEDADN-NCVYRHEIVH-APSEQTMVLTDLSADPTLPRTTTA-CPRCGYQEAVFFQS 88
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BLAST of mRNA_Ecto-sp13_S_contig20028.6534.1 vs. uniprot
Match: A0A1B8ABA8_FUSPO (DNA-directed RNA polymerase subunit n=29 Tax=Fusarium TaxID=5506 RepID=A0A1B8ABA8_FUSPO)

HSP 1 Score: 85.5 bits (210), Expect = 2.330e-19
Identity = 42/92 (45.65%), Postives = 59/92 (64.13%), Query Frame = 1 
Query:    1 IVFSLCLRSNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLITTAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQADE 276
            I F  C   +N+++PKE++   KL ++C  C+Y EDA+S +CV+RNNL T+AG    + Q DV  DPTL RS    C  C + EAVFFQ+ +
Sbjct:   22 ITFRFCSECSNMLYPKEDQDAHKLQFTCRTCQYTEDAKS-TCVFRNNLNTSAGETAGVTQ-DVGSDPTLPRSTKT-CPSCQHTEAVFFQSQQ 110
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig20028.6534.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LE56_ECTSI2.540e-5596.55TFIIS-type domain-containing protein n=1 Tax=Ectoc... [more]
A0A835YYP5_9STRA4.320e-3565.52DNA-directed RNA polymerase subunit n=1 Tax=Tribon... [more]
A0A8H7VS23_9FUNG5.580e-2248.91DNA-directed RNA polymerase subunit n=1 Tax=Mucor ... [more]
K0THW0_THAOC1.980e-2146.15TFIIS-type domain-containing protein n=1 Tax=Thala... [more]
A0A162XV76_PHYB84.540e-2145.65DNA-directed RNA polymerase subunit n=1 Tax=Phycom... [more]
A0A7S3NJ32_9STRA9.820e-2146.51Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A8B7CCB1_PHODC7.410e-2047.73DNA-directed RNA polymerase subunit n=3 Tax=Arecac... [more]
A0A1X2HED9_SYNRA1.490e-1944.57DNA-directed RNA polymerase subunit n=1 Tax=Syncep... [more]
A0A8H7UCI9_9FUNG1.490e-1945.45DNA-directed RNA polymerase subunit n=2 Tax=Umbelo... [more]
A0A1B8ABA8_FUSPO2.330e-1945.65DNA-directed RNA polymerase subunit n=29 Tax=Fusar... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig20028contigEcto-sp13_S_contig20028:663..1638 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score180.3
Seed ortholog evalue4.4e-43
Seed eggNOG ortholog2880.D8LE56
Preferred namePOLR2I
Model size288
KEGG rclassRC02795
KEGG koko:K03017
KEGG ReactionR00435,R00441,R00442,R00443
KEGG Pathwayko00230,ko00240,ko01100,ko03020,ko05016,ko05169,map00230,map00240,map01100,map03020,map05016,map05169
KEGG ModuleM00180
Hectar predicted targeting categoryother localisation
GOsGO:0000375,GO:0000377,GO:0000398,GO:0000428,GO:0001192,GO:0001193,GO:0003674,GO:0003824,GO:0003899,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005665,GO:0006139,GO:0006259,GO:0006281,GO:0006283,GO:0006289,GO:0006351,GO:0006352,GO:0006354,GO:0006366,GO:0006367,GO:0006368,GO:0006370,GO:0006396,GO:0006397,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0007154,GO:0007165,GO:0007166,GO:0007167,GO:0007169,GO:0008150,GO:0008152,GO:0008270,GO:0008380,GO:0008543,GO:0009058,GO:0009059,GO:0009301,GO:0009452,GO:0009719,GO:0009987,GO:0010033,GO:0010467,GO:0010468,GO:0016070,GO:0016071,GO:0016073,GO:0016591,GO:0016740,GO:0016772,GO:0016779,GO:0018130,GO:0019222,GO:0019438,GO:0023052,GO:0030880,GO:0031974,GO:0031981,GO:0032774,GO:0032991,GO:0033554,GO:0034062,GO:0034641,GO:0034645,GO:0034654,GO:0034660,GO:0036260,GO:0042221,GO:0042795,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044344,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044451,GO:0044464,GO:0046483,GO:0046872,GO:0046914,GO:0050789,GO:0050794,GO:0050896,GO:0051716,GO:0055029,GO:0060147,GO:0060255,GO:0060964,GO:0060966,GO:0060968,GO:0061695,GO:0065007,GO:0070013,GO:0070848,GO:0070887,GO:0071310,GO:0071363,GO:0071495,GO:0071704,GO:0071774,GO:0090304,GO:0097659,GO:0097747,GO:0098781,GO:0140098,GO:1901360,GO:1901362,GO:1901576,GO:1902494,GO:1990234
Exons2
EggNOG free text desc.Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and or for immediate growth after restoration of oxygen
EggNOG OGsCOG1594@1,KOG2691@2759
Ec32 ortholog descriptionZinc finger, TFIIS-type
Ec32 orthologEc-00_001020.1
Cds size288
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01611,ko00000,ko00001,ko00002,ko03021,ko03400
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463008.2111216-CDS-Ecto-sp13_S_contig20028:662..7921681463008.2111216-CDS-Ecto-sp13_S_contig20028:662..792Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig20028 663..792 -
1681463008.225389-CDS-Ecto-sp13_S_contig20028:1480..16381681463008.225389-CDS-Ecto-sp13_S_contig20028:1480..1638Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig20028 1481..1638 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig20028.6534.1prot_Ecto-sp13_S_contig20028.6534.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig20028 663..1638 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig20028.6534.1

>prot_Ecto-sp13_S_contig20028.6534.1 ID=prot_Ecto-sp13_S_contig20028.6534.1|Name=mRNA_Ecto-sp13_S_contig20028.6534.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=96bp
IVFSLCLRSNNIMHPKENKTDRKLMYSCNRCKYEEDAQSGSCVYRNNLIT
TAGNKLDIVQTDVVDDPTLQRSKNAICEKCNYNEAVFFQADEVRP*
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mRNA from alignment at Ecto-sp13_S_contig20028:663..1638-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig20028.6534.1 ID=mRNA_Ecto-sp13_S_contig20028.6534.1|Name=mRNA_Ecto-sp13_S_contig20028.6534.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=976bp|location=Sequence derived from alignment at Ecto-sp13_S_contig20028:663..1638- (Ectocarpus species13 EcNAP12_S_4_19m)
ATTGTGTTTTCCCTGTGCCTTCGCAGCAATAACATCATGCATCCGAAAGA
GAACAAGACGGACCGGAAGTTGATGTATTCATGCAATCGGTGTAAATATG
AGGAGGATGCACAATCGGGATCGTGCGTCTACAGGAATAACCTCATCACT
ACCGCAGGGTGAGACATGGTGCAGAAGAGAGATCGAGGCAGTATTTTCTA
TGGTACAAGGCGTACAGTTTGAAATATTTTGCTGCCATGTTTGCACCTTG
AGTACGATGCCTTCGTTTCGTTTCCCATGTACATGTAGAGACAGCCATGT
AAACACGCGGATCTACCGATACTGAAACCGCTTGTTCGAGTGCGGTGGAT
TGTTGGTGTTATGGTTAGCTCCTGTTTCTACAACGGCCCACCTGTGACGA
AAGCAAGTCTTGGGGCTGTTCTTAATGGGGTGGGGGTGGGTGTTCCTTCG
TGCTTTTGCGGCGCGTGCTCAACGGGCGTATGTAATATGTCGCCATGCCC
ATAGTGTGTGCAGCTGCAAACGCACGCACGCCCGGCTTTAGCCTGCGCGG
TTGTCATAGCGGTATTTTTTGCAAGACGGGAACACGGCAGCCTCGAATTA
CGTCTGGTCGTCGAGATAGTACATCTTGATGAAAATCTCTCGTGTCGTGA
AGTTATGCAGGCCCAGTGGTGTCCTCCGGTATGAAATGTGCCTCAAACTC
TTCTGTGGCAAACTTCACACCCAGTGAAGGCATGTCGGTGTTCGTCGCGT
TTGCTGCTGGGACGCCGAAGTGTGGCAAGCTTGTTCCCAATACGCAATAT
GCATGTCGCCGACTGTCCTATGCTGTTTTTCGCTCATTCGTGTCAGGAAT
AAGCTAGATATTGTGCAGACCGATGTCGTGGACGACCCCACCTTGCAACG
AAGCAAGAATGCCATCTGCGAGAAATGTAACTACAACGAAGCCGTCTTCT
TCCAAGCAGACGAGGTGCGTCCCTAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig20028:663..1638-

>mRNA_Ecto-sp13_S_contig20028.6534.1 ID=mRNA_Ecto-sp13_S_contig20028.6534.1|Name=mRNA_Ecto-sp13_S_contig20028.6534.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=288bp|location=Sequence derived from alignment at Ecto-sp13_S_contig20028:663..1638- (Ectocarpus species13 EcNAP12_S_4_19m)
ATTGTGTTTTCCCTGTGCCTTCGCAGCAATAACATCATGCATCCGAAAGA
GAACAAGACGGACCGGAAGTTGATGTATTCATGCAATCGGTGTAAATATG
AGGAGGATGCACAATCGGGATCGTGCGTCTACAGGAATAACCTCATCACT
ACCGCAGGGAATAAGCTAGATATTGTGCAGACCGATGTCGTGGACGACCC
CACCTTGCAACGAAGCAAGAATGCCATCTGCGAGAAATGTAACTACAACG
AAGCCGTCTTCTTCCAAGCAGACGAGGTGCGTCCCTAG
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