mRNA_Ecto-sp13_S_contig11722.1488.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig11722.1488.1
Unique NamemRNA_Ecto-sp13_S_contig11722.1488.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig11722.1488.1 vs. uniprot
Match: D8LET7_ECTSI (Peptidylprolyl isomerase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LET7_ECTSI)

HSP 1 Score: 339 bits (870), Expect = 4.060e-113
Identity = 199/210 (94.76%), Postives = 202/210 (96.19%), Query Frame = 1 
Query:    1 MSPCKSTACVALAAVITLIAGVSDAFVVPSASVLSASKSDSRAPGSSSSSSLSMGADGGTTRGKDLVFQTIATLLAGVTAAANFESANAANKLQVSSTASGSRVNKDPISLLQWGLPIDCKPARQLQEAVEAAKLDLFQKNWSRAVSNCSKAKSAMSGNNKAAILKAVGSSNEAGATKLIADIDMGLGALQRIVQDDGAGVVLEKAKLDE 630
            MSPCKSTACVALAAV TLIAGVSDAFVVPSASVL AS+S+SRAPGSSSSSSLSMGADGGTTRG D   QTIATLLAGVTAAANFESANAANKLQVSSTASGSRVNKDPISLLQWGLPIDCKPARQLQE VEAAKLDLFQKNWSRAVSNCSKAKSAMSGN KAAILKAVGSSNEAGATKL+ADIDMGLGALQRIVQDDGAGVVLEKAKLDE
Sbjct:    1 MSPCKSTACVALAAVSTLIAGVSDAFVVPSASVLGASRSNSRAPGSSSSSSLSMGADGGTTRG-DFGKQTIATLLAGVTAAANFESANAANKLQVSSTASGSRVNKDPISLLQWGLPIDCKPARQLQEVVEAAKLDLFQKNWSRAVSNCSKAKSAMSGN-KAAILKAVGSSNEAGATKLMADIDMGLGALQRIVQDDGAGVVLEKAKLDE 208
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BLAST of mRNA_Ecto-sp13_S_contig11722.1488.1 vs. uniprot
Match: A0A836CBT3_9STRA (Peptidylprolyl isomerase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CBT3_9STRA)

HSP 1 Score: 85.5 bits (210), Expect = 5.990e-16
Identity = 55/125 (44.00%), Postives = 81/125 (64.80%), Query Frame = 1 
Query:  259 ANAANKLQVSSTASGSRVNKDPISLLQWGLPIDCKPARQLQEAVEAAKLDLFQKNWSRAVSNCSKAKSAMSGNNKAAILKAVGSSNEAGATKLIADIDMGLGALQRIVQD-DGAGVVLEKAKLDE 630
            A AA+    S+T SGSRVNKDP SLL+ GLPI+CKP   LQEA+++ K D ++K+WSRA+++C +A   +S   K  +L AV  + +A   +L+  I   L  L+ I++  +G G V E+ KLD+
Sbjct:   43 AQAASGRGPSNTGSGSRVNKDPQSLLRQGLPINCKPVFDLQEAIDSVKYDTYRKDWSRALNDCKQAGGIVSKKRKD-LLAAVRPTEKAKGEQLLQTILDKLTPLEAILKSKEGTGTVAERIKLDD 166
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BLAST of mRNA_Ecto-sp13_S_contig11722.1488.1 vs. uniprot
Match: A0A6T5MD62_HETAK (Peptidylprolyl isomerase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6T5MD62_HETAK)

HSP 1 Score: 75.9 bits (185), Expect = 1.630e-12
Identity = 48/114 (42.11%), Postives = 70/114 (61.40%), Query Frame = 1 
Query:  292 TASGSRVNKDPISLLQWGLPIDCKPARQLQEAVEAAKLDLFQKNWSRAVSNCSKAKSAMSGNNKAAILKAVGSSNEAGATKLIADIDMGLGALQRIVQD-DGAGVVLEKAKLDE 630
            T SG+RVNKDP SLL++GLPI+ K AR+LQ AVE  K D+  K W   ++   KA++ +    +A +LK V  S +A   K I ++   L  L++I+   +G G V E+ KLD+
Sbjct:   98 TGSGTRVNKDPESLLRYGLPINSKKARELQAAVEGVKSDILIKRWGAGIAGVQKARTVLG--KEAELLKEVRPSAQAEGKKAIQELKDLLDPLEQILASKEGTGTVQEREKLDK 209
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BLAST of mRNA_Ecto-sp13_S_contig11722.1488.1 vs. uniprot
Match: A0A7S2WU08_9STRA (Hypothetical protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WU08_9STRA)

HSP 1 Score: 69.3 bits (168), Expect = 1.140e-11
Identity = 47/113 (41.59%), Postives = 73/113 (64.60%), Query Frame = 1 
Query:  292 TASGSRVNKDPISLLQWGLPIDCKPARQLQEAVEAAKLDLFQKNWSRAVSNCSKAKSAMSGNNKAAILKAVGSSNEAGATKLIADIDMGLG-ALQRIVQDDGAGVVLEKAKLD 627
            TA+GSRVNKDP SLL+ GLPI+ +P R+LQ A+E A+ DL  K  S ++ +  +A+  +  N   A+LK+V  S +  A +L+ +ID  L  A+  + +++G G + E+A LD
Sbjct:   14 TAAGSRVNKDPESLLRNGLPINNEPVRKLQVALETARNDLKVKRISASIGDVQQARGIIK-NQATALLKSVRPSAQERAKELLEEIDAALDPAITALKENNGQGSLQERAALD 125
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BLAST of mRNA_Ecto-sp13_S_contig11722.1488.1 vs. uniprot
Match: W7TQJ8_9STRA (Peptidylprolyl isomerase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TQJ8_9STRA)

HSP 1 Score: 65.1 bits (157), Expect = 8.350e-9
Identity = 50/126 (39.68%), Postives = 75/126 (59.52%), Query Frame = 1 
Query:  253 ESANAANKLQVSSTASGSRVNKDPISLLQWGLPIDCKPARQLQEAVEAAKLDLFQKNWSRAVSNCSKAKSAMSGNNKAAILKAVGSSNEAGATKLIADIDMGLGALQR-IVQDDGAGVVLEKAKLD 627
            +SA+AA      S+A GSRVNKD  SLL++GLPI+ K AR+LQEAVE  K ++  K  +    +  +AKS ++ + +  +LKAV    +  A +L+ +++  L  L     +D G G   E+AKLD
Sbjct:   96 QSAHAARPSNAPSSA-GSRVNKDAESLLRYGLPINSKEARELQEAVEGVKENIRGKRLNEGKKDVQRAKSILA-SKREKLLKAVRPDGKERAQELLKELEGNLDELTATFAEDAGRGSEQERAKLD 219
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig11722.1488.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
D8LET7_ECTSI4.060e-11394.76Peptidylprolyl isomerase n=1 Tax=Ectocarpus silicu... [more]
A0A836CBT3_9STRA5.990e-1644.00Peptidylprolyl isomerase n=1 Tax=Tribonema minus T... [more]
A0A6T5MD62_HETAK1.630e-1242.11Peptidylprolyl isomerase n=1 Tax=Heterosigma akash... [more]
A0A7S2WU08_9STRA1.140e-1141.59Hypothetical protein (Fragment) n=1 Tax=Rhizochrom... [more]
W7TQJ8_9STRA8.350e-939.68Peptidylprolyl isomerase n=2 Tax=Monodopsidaceae T... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig11722contigEcto-sp13_S_contig11722:198..4210 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score341.7
Seed ortholog evalue2.5e-91
Seed eggNOG ortholog2880.D8LET7
Preferred nameTLP40
Model size630
Hectar predicted targeting categorychloroplast
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006950,GO:0006952,GO:0008150,GO:0009507,GO:0009532,GO:0009534,GO:0009535,GO:0009536,GO:0009570,GO:0009579,GO:0009605,GO:0009607,GO:0009617,GO:0016020,GO:0031976,GO:0031977,GO:0031984,GO:0034357,GO:0042651,GO:0042742,GO:0043207,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044436,GO:0044444,GO:0044446,GO:0044464,GO:0050896,GO:0051704,GO:0051707,GO:0055035,GO:0098542
Exons6
EggNOG free text desc.peptidyl-prolyl cis-trans isomerase activity
EggNOG OGs2QSSP@2759,COG0652@1
Ec32 ortholog descriptionPeptidyl-prolyl cis-trans isomerase
Ec32 orthologEc-21_005540.1
Cds size630
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig11722.1488.1prot_Ecto-sp13_S_contig11722.1488.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig11722 198..4210 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462617.4611971-CDS-Ecto-sp13_S_contig11722:197..2651681462617.4611971-CDS-Ecto-sp13_S_contig11722:197..265Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11722 198..265 -
1681462617.4709945-CDS-Ecto-sp13_S_contig11722:690..7941681462617.4709945-CDS-Ecto-sp13_S_contig11722:690..794Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11722 691..794 -
1681462617.479092-CDS-Ecto-sp13_S_contig11722:1341..14331681462617.479092-CDS-Ecto-sp13_S_contig11722:1341..1433Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11722 1342..1433 -
1681462617.4878168-CDS-Ecto-sp13_S_contig11722:2144..22101681462617.4878168-CDS-Ecto-sp13_S_contig11722:2144..2210Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11722 2145..2210 -
1681462617.495608-CDS-Ecto-sp13_S_contig11722:2921..30171681462617.495608-CDS-Ecto-sp13_S_contig11722:2921..3017Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11722 2922..3017 -
1681462617.505973-CDS-Ecto-sp13_S_contig11722:4006..42101681462617.505973-CDS-Ecto-sp13_S_contig11722:4006..4210Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11722 4007..4210 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig11722.1488.1

>prot_Ecto-sp13_S_contig11722.1488.1 ID=prot_Ecto-sp13_S_contig11722.1488.1|Name=mRNA_Ecto-sp13_S_contig11722.1488.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=210bp
MSPCKSTACVALAAVITLIAGVSDAFVVPSASVLSASKSDSRAPGSSSSS
SLSMGADGGTTRGKDLVFQTIATLLAGVTAAANFESANAANKLQVSSTAS
GSRVNKDPISLLQWGLPIDCKPARQLQEAVEAAKLDLFQKNWSRAVSNCS
KAKSAMSGNNKAAILKAVGSSNEAGATKLIADIDMGLGALQRIVQDDGAG
VVLEKAKLDE
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mRNA from alignment at Ecto-sp13_S_contig11722:198..4210-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig11722.1488.1 ID=mRNA_Ecto-sp13_S_contig11722.1488.1|Name=mRNA_Ecto-sp13_S_contig11722.1488.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=4013bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11722:198..4210- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGTCGCCGTGCAAGTCGACGGCGTGCGTTGCACTGGCGGCGGTTATCAC
TCTGATTGCGGGCGTCTCCGATGCGTTCGTAGTACCGTCAGCTTCGGTAC
TCAGCGCGTCGAAGTCCGACAGCCGAGCCCCAGGCAGCAGCAGCAGCAGC
TCCCTGTCCATGGGCGCCGACGGGGGCACCACCCGAGGCAAGGATCTGGT
CTTTGTAGGCTTCGCTGGCGTCTGCCGAGAGTTTCGGTGCTGCATGATTT
GGTTCTCTCGCACTTGTATTTGGAACGCATGTGCTGTTTGTTGATGGTGT
GAAAGGGGGACATATTCTAAGACACATGTTTTGAGTCAGAAGCAGTCTGT
CAATCCTTCCTTCTCCGGTGACGAGAGCATGAACCGGCTCTATGTACTAT
ATACCTCCCACCGCACGTTCTTAATGCCAGACCACGAAGACGCGCTGCCG
GAGTAAAGGTTGCCGGCGTTGCTGTTGTTGCTTCCGCGCCGCATTGTTGA
GTCGAGGTCTAAAGATCGGAGAAAGGGTATAGTCTACATCTTCAACCCAG
AAGAATGTTTCAACGTCCAACATTCACTCCAACACCGGCTGTATTGCTCC
CCGAGGAGGGAGGATGCCTCCCTCTACCTTCCGACACACATGGTATCGTA
GTGGAATTTTCTTGACGATATGGTCTCAGAGACGGCCTTCTGGATCATTG
GCACTGCTGTTGTGCCAGCTGTTGGGCTAGCAGCCGAGCGGGGGTCGTAT
ATCCTGGGTATCGCACCCACCGAGCGACCGATAAACAAGTACTAATAGCA
GATAGATAGAGATGTACAAGTAGCAGATAGGCATGCGTGAAGCACGTTTA
CTGTGCCCGAAGTAGATACCACCTGCTAGGTTGAGGAATGCACACACATG
ACACGTGGACAAGCCAGGAACCTGACCAGCAAGGTGTGTATAGTCTATGA
CACACGAGTCGAATATTTTTTGCGACCCACTATGCTATTGGTGTATGTAG
CCAAGCCGGAATCTTGTGTACAGCAGTACCTATCAGGGCCGTTGTGTGTT
TGCCGCTCCATTGTGGTAGCCTCTCCGCCTTCTGCGAACTCTCGAGCGTC
GCGAGATAACGATACGGCCCCGTCGCTCAAACAACAAGATGGTTACACGT
TTGTTGTCATTGGTCTCGTGTTGAAACAGGGGACTTCGGGAAGCAGACGA
TCGCCACGCTTCTCGCCGGGGTCACGGCCGCGGCCAACTTCGAGTCGGCG
AACGCTGCCAACAAGCTCCAAGTCAGCTCCACGGCATCGGTAAGAGAGAA
CAAGAGTAGCGGTAACCGTGTCTTCGGATGGCCTTTGCTTAGCATGGCGG
AACTGCAAACAATTTTTTGCCTGGTGAGATGTTGGTTCGCTTCGCTGCTC
AACAAGGCTTCTTCCCTGTGTGGATCTNNNNNNCGTGTGGATCTCCGCCG
CTGCATGGGTGACATCGGCATTAGCTTTTGACGGCAACGAGCGCAGCTTG
CACCAGCGTAGTACATACCGCCATCGTTCCAGCACGAGGCAAATGCTTGG
TACCGTCCGCGCTCTGCTGCTCTGTGCTTGCTTCAACCAAAGAATCTCGG
AGCTGTTCTAAGAAGCTATGTGCTACACCTGTCTGTCCGTCGTGAACGTA
GAAATGGGATACTGCAGCTCGGCTTGAAACGTCGCTCACTCACAAGTTGT
TATCAACATTTTCCTGCGGCAGTGAGTACCTCTCGTGGTATGTCCCCCCG
CTGGCCTCCCTGCTGCATCTTCCGTGCCAAACAATCCTGTTGCTGTGTCG
AAAGGCGAACGTCCTCGGCTCTCACGTACCAGCGTAAGCGACAAGCCCCA
TGTTGACAGGAATAAATGTGCGGGCGTGGGAGTGCCCTCTGGCCCGTACC
CCCCATCACACCACTACTAATGTTTCTGATGCAATAATCTTGCGCAATGT
TCGCTCCCTCCGGTGCTGCCACGAACGGACTGGTCCGGCGACACGCGTAG
GGGTCCCGAGTCAACAAGGATCCGATCTCGTTGCTTCAGTGGGGCCTCCC
GATAGACTGCAAGCCGGTGAGAAGCGAGAGTAGGGTCTACCAACACTGCA
GTTTGGTCCTGCTCTTGTCCGACGCTTGGCGGTCCTGGCGCTACGCCACA
CAACCGAAAGAACATGCAGCAGGAGACCCGAGAAAAAGGAAAAACCTTGT
ACTTTTGACTGGAGCGGTCAAGAAGTCGTGCGCTGGTTGTGGGTGTCTTG
TGGTGGCTGATTGGAAGGAATGGTGTGCCCGAGATCTCTCTCGCAGGGCT
GTTGATGACGTTTTTTGTCAGCCTTCTGCCATCGCATTTGCCGCGTGGTT
GTCGCTTTTTGCTACACCGCGGTGAGCGTATCTCCGACCCGTCCAGGCGG
CCGTAGTAGGCGCTGATTTTTGAGGCGTTCGCGGCACGATTAAACGGAAC
CCCCCAGCCCTCTAGCCAGCTTGCACAGCTTCTATCGATCGATCCGCTTG
ATGGCTTTACTCCGTTTGAGTGCCAACTCCAAAGTCGATCCGCGTGCTGG
TGGTTTACCCTCGCGACGGGGTGGGTACGGTGGTTGTTGTCGTTTGTAAA
GTGTCCAAGTTGGTCCTTCATCGGGAGTACCCGGCGGACTTGGATCCGTC
ACGTCGCGGATATTGCCGCGGTCTCTTGGGGGGACCGCTATCGTCGAAAA
TGGGGACCTTGATGCCCCTACAAGTTTTTTTGCGGGTGGTGACTCGTGGC
CCGAAACCTGGTTTCCGGCGAAAACAGGCTCGGCAGCTGCAGGAGGCGGT
GGAGGCGGCTAAGCTGGATCTCTTCCAGAAGAACTGGTCACGCGCCGTTT
CCAACTGCTCAAAGGCGAAGTGAGCCCCGGTCGGTAGTTGATGAACCTAT
GTGCCTCCCCGTGGGATGCGCCTGACCGCACGTGCATTGCCTGCACCTCG
CTCAAAGCAAGTTGGATGGTTGGCTAATCGCTAATCGAAACGCACAGAAA
TGGGAGGGGGGGGAGTTTGCGCTGCTGTTGTTCCAACCAAGAGTCGTTCC
TGCAGCCATGCGTGGCTTTATCCCCTTGCAGGAAACCTGCCATGCACGAA
GCGTTGATGATTTGGCATGTTTGGGGCTGAGAAGTCAATGCGCTTTGAAG
GATCTGTGCTGCCTTTGTCGCGGTGCACCTGTCGCTCTTCGCCGTCGCCC
CCGGCGTCTGCGCTGTTGCCATGCTGCCTCTTCACAAGCTCGAACTTTGT
GCTTGTGCGCACATTGTGCCTCCAAATGCCCCCGGTGTAGTTTCTGCCTC
CACACATCGACGAAACAAAACACACGAACGCGCTCGCGCGCGTGCCTGAC
AAGGTCACAGTTCTCCGCCGTAATAGCTCAGTGTTTCGGTGCTGCGAACG
ACGTTGCTCTGTTTAGGTCTGCTATGTCAGGCAACAACAAAGCGGCGATC
CTCAAAGCGGTGGGCTCGAGCAACGAGGCAGGGGCAACCAAGCTCATAGC
GGACATCGACATGGGTCTTGGTGAGAACCACTTTCTAAGATTATGCCTCC
TTACTGTCGTTTTGACTGTTTTGGGAACGGTTCCAAGACGGGTCAGGCAC
GTTGCCTGGGAGGGAGGGAGGGAGAGAGGGAGGGAGGGAGGAATGGCCTG
CGTCTTGTCCTTGCCCTTGACGACGATGCGCGTTCTACCACTGTTCAAGT
GCTGCTCCGTTGCCATTTTCCTGCCACCGTTCTTGTTGCTGACACTGCTG
GCACTCCTGGCACTGCTGGTACTGCTGGTACTGCTGGTGCTGCTGCTGGT
AAATACTGCTGCTCCCGCTGATAGCCATCGTCATAATTGCTGCGCTATTG
CTTTGTTCTGCCGGTGCGGCTTGTGATGCCGTCCTCAACTGCTGCTCGTG
GTTTCTGCGACTCCCGTGTTTTTGTTTGCTGTCGCTCCGTCCTAGGCGCT
CTGCAGAGAATCGTCCAGGACGATGGAGCTGGGGTGGTGTTAGAGAAGGC
TAAGCTAGACGAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig11722:198..4210-

>mRNA_Ecto-sp13_S_contig11722.1488.1 ID=mRNA_Ecto-sp13_S_contig11722.1488.1|Name=mRNA_Ecto-sp13_S_contig11722.1488.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=630bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11722:198..4210- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGTCGCCGTGCAAGTCGACGGCGTGCGTTGCACTGGCGGCGGTTATCAC
TCTGATTGCGGGCGTCTCCGATGCGTTCGTAGTACCGTCAGCTTCGGTAC
TCAGCGCGTCGAAGTCCGACAGCCGAGCCCCAGGCAGCAGCAGCAGCAGC
TCCCTGTCCATGGGCGCCGACGGGGGCACCACCCGAGGCAAGGATCTGGT
CTTTCAGACGATCGCCACGCTTCTCGCCGGGGTCACGGCCGCGGCCAACT
TCGAGTCGGCGAACGCTGCCAACAAGCTCCAAGTCAGCTCCACGGCATCG
GGGTCCCGAGTCAACAAGGATCCGATCTCGTTGCTTCAGTGGGGCCTCCC
GATAGACTGCAAGCCGGCTCGGCAGCTGCAGGAGGCGGTGGAGGCGGCTA
AGCTGGATCTCTTCCAGAAGAACTGGTCACGCGCCGTTTCCAACTGCTCA
AAGGCGAAGTCTGCTATGTCAGGCAACAACAAAGCGGCGATCCTCAAAGC
GGTGGGCTCGAGCAACGAGGCAGGGGCAACCAAGCTCATAGCGGACATCG
ACATGGGTCTTGGCGCTCTGCAGAGAATCGTCCAGGACGATGGAGCTGGG
GTGGTGTTAGAGAAGGCTAAGCTAGACGAG
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