mRNA_Ecto-sp13_S_contig11576.1372.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig11576.1372.1
Unique NamemRNA_Ecto-sp13_S_contig11576.1372.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig11576.1372.1 vs. uniprot
Match: A0A6H5JPR9_9PHAE (Aconitase_C domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JPR9_9PHAE)

HSP 1 Score: 251 bits (641), Expect = 1.920e-82
Identity = 131/136 (96.32%), Postives = 134/136 (98.53%), Query Frame = 1 
Query:    1 MMWGFSTTATMVAAVSSSVSAFVVPMPTAGTTAVASRPGCKSPAGVAPRRAAEADAAAIDDPRVKEMPKNRDRPDKYTGQCFVVKDNIDTDQIIPAEYLTLVPSNADEYEKLGSYAMIGLPDVYTTRYVEEGSMAT 408
            MMWGF+TTATMVAAVSSSVSAF VP+PTAGTTAVASRPGCKS AGVAPRRAAEADAAAIDDPRVKEMPKNRDRPDKYTGQCFVVKDNIDTDQIIPAEYLTLVPSNA+EYEKLGSYAMIGLPDVYTTRYVEEGSMAT
Sbjct:    1 MMWGFATTATMVAAVSSSVSAFFVPVPTAGTTAVASRPGCKSSAGVAPRRAAEADAAAIDDPRVKEMPKNRDRPDKYTGQCFVVKDNIDTDQIIPAEYLTLVPSNANEYEKLGSYAMIGLPDVYTTRYVEEGSMAT 136
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BLAST of mRNA_Ecto-sp13_S_contig11576.1372.1 vs. uniprot
Match: B7ZGN7_9PHAE (3-isopropylmalate dehydratase (Fragment) n=1 Tax=Laminaria digitata TaxID=80365 RepID=B7ZGN7_9PHAE)

HSP 1 Score: 156 bits (394), Expect = 4.290e-45
Identity = 92/135 (68.15%), Postives = 101/135 (74.81%), Query Frame = 1 
Query:    4 MWGFSTTATMVAAVSSSVSAFVVPMPTAGTTAVASRPGCKSPAGVAPRRAAEADAAAIDDPRVKEMPKNRDRPDKYTGQCFVVKDNIDTDQIIPAEYLTLVPSNADEYEKLGSYAMIGLPDVYTTRYVEEGSMAT 408
            M GF+   TMVA+V+S   AFV P+  AG  A A+RP C+S   VA + AA  D     DPR KEMPK R+RP KYTG CFVVKDNIDTDQIIPAEYLTLVPS ADEYEKLGSYAMIGLP VY TR+VEEGSM T
Sbjct:    3 MLGFAVALTMVASVTSRAGAFVPPISAAG--AAATRPACQS--SVARQAAASDDM----DPREKEMPKFRERPTKYTGACFVVKDNIDTDQIIPAEYLTLVPSKADEYEKLGSYAMIGLPSVYKTRFVEEGSMKT 129
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BLAST of mRNA_Ecto-sp13_S_contig11576.1372.1 vs. uniprot
Match: W7SZS5_9STRA (3-isopropylmalate dehydratase (Fragment) n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7SZS5_9STRA)

HSP 1 Score: 105 bits (262), Expect = 1.720e-26
Identity = 52/79 (65.82%), Postives = 61/79 (77.22%), Query Frame = 1 
Query:  175 IDDPRVKEMPKNRDRPDKYTGQCFVVKDNIDTDQIIPAEYLTLVPSNADEYEKLGSYAMIGLPD-VYTTRYVEEGSMAT 408
            +DDPR+ EMPK R RP +Y G+ FV +DNIDTDQIIPAEYLTLVPS  DEYE+LGSYA+IGLP  +Y   YV+ G   T
Sbjct:   44 VDDPRLLEMPKFRPRPRQYRGKAFVARDNIDTDQIIPAEYLTLVPSKTDEYEQLGSYALIGLPQALYPVPYVQPGHTKT 122
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BLAST of mRNA_Ecto-sp13_S_contig11576.1372.1 vs. uniprot
Match: A0A7J7Q700_9CHLO (Small subunit of isopropylmalate dehydratase n=1 Tax=Scenedesmus sp. NREL 46B-D3 TaxID=2650976 RepID=A0A7J7Q700_9CHLO)

HSP 1 Score: 104 bits (260), Expect = 2.400e-25
Identity = 50/65 (76.92%), Postives = 53/65 (81.54%), Query Frame = 1 
Query:  214 DRPDKYTGQCFVVKDNIDTDQIIPAEYLTLVPSNADEYEKLGSYAMIGLPDVYTTRYVEEGSMAT 408
            D P    G CFV KDNIDTDQIIPAEYLTLVPS  DEYEKLGSYA+IGLPD YTT++VEEG M T
Sbjct:   31 DAPRIVRGTCFVTKDNIDTDQIIPAEYLTLVPSKPDEYEKLGSYALIGLPDEYTTKFVEEGEMKT 95
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BLAST of mRNA_Ecto-sp13_S_contig11576.1372.1 vs. uniprot
Match: A0A2J8A327_9CHLO (3-isopropylmalate dehydratase small subunit n=1 Tax=Tetrabaena socialis TaxID=47790 RepID=A0A2J8A327_9CHLO)

HSP 1 Score: 101 bits (251), Expect = 1.010e-24
Identity = 49/69 (71.01%), Postives = 55/69 (79.71%), Query Frame = 1 
Query:  205 KNRDRPDKYTGQCFVVKDNIDTDQIIPAEYLTLVPSNADEYEKLGSYAMIGLPD-VYTTRYVEEGSMAT 408
            K    P   +G+C+V +DNIDTDQIIPAEYLTLVPS  DEYEKLGSYA+IGLPD  YTTRYV+EG M T
Sbjct:   32 KKEGEPRVISGKCYVTRDNIDTDQIIPAEYLTLVPSKPDEYEKLGSYALIGLPDDTYTTRYVQEGQMKT 100
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BLAST of mRNA_Ecto-sp13_S_contig11576.1372.1 vs. uniprot
Match: A8HYG8_CHLRE (Isopropylmalate dehydratase, small subunit n=4 Tax=Chlamydomonas TaxID=3052 RepID=A8HYG8_CHLRE)

HSP 1 Score: 101 bits (252), Expect = 3.770e-24
Identity = 50/64 (78.12%), Postives = 53/64 (82.81%), Query Frame = 1 
Query:  220 PDKYTGQCFVVKDNIDTDQIIPAEYLTLVPSNADEYEKLGSYAMIGLPD-VYTTRYVEEGSMAT 408
            P    G+CFV KDNIDTDQIIPAEYLTLVPS  DEYEKLGSYA+IGLPD  YTTRYV+EG M T
Sbjct:   33 PRVVRGKCFVTKDNIDTDQIIPAEYLTLVPSKPDEYEKLGSYALIGLPDDTYTTRYVKEGEMKT 96
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BLAST of mRNA_Ecto-sp13_S_contig11576.1372.1 vs. uniprot
Match: A0A4D9CY72_9STRA (Uncharacterized protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CY72_9STRA)

HSP 1 Score: 106 bits (264), Expect = 4.370e-24
Identity = 52/79 (65.82%), Postives = 62/79 (78.48%), Query Frame = 1 
Query:  175 IDDPRVKEMPKNRDRPDKYTGQCFVVKDNIDTDQIIPAEYLTLVPSNADEYEKLGSYAMIGLPD-VYTTRYVEEGSMAT 408
            +DDPR+ EMPK R RP +Y G+ FV +DNIDTDQIIPAEYLTLVPS  DEYE+LGSYA+IGLP+ +Y   YV+ G   T
Sbjct:   44 VDDPRLLEMPKFRPRPRQYRGKAFVARDNIDTDQIIPAEYLTLVPSKTDEYEQLGSYALIGLPEALYPVPYVQPGHTKT 122
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BLAST of mRNA_Ecto-sp13_S_contig11576.1372.1 vs. uniprot
Match: A0A836BR91_9CHLO (Aconitase_C domain-containing protein n=1 Tax=Edaphochlamys debaryana TaxID=47281 RepID=A0A836BR91_9CHLO)

HSP 1 Score: 101 bits (251), Expect = 5.310e-24
Identity = 51/69 (73.91%), Postives = 55/69 (79.71%), Query Frame = 1 
Query:  205 KNRDRPDKYTGQCFVVKDNIDTDQIIPAEYLTLVPSNADEYEKLGSYAMIGLPDV-YTTRYVEEGSMAT 408
            K  + P    G+CFV KDNIDTDQIIPAEYLTLVPS  DEYEKLGSYA+IGLPDV Y TRYV +GSM T
Sbjct:   28 KKPNEPRIIRGKCFVTKDNIDTDQIIPAEYLTLVPSKPDEYEKLGSYALIGLPDVEYPTRYVNDGSMKT 96
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BLAST of mRNA_Ecto-sp13_S_contig11576.1372.1 vs. uniprot
Match: A6XGU6_9CHLO (3-isopropylmalate dehydratase (Fragment) n=1 Tax=Polytomella parva TaxID=51329 RepID=A6XGU6_9CHLO)

HSP 1 Score: 98.6 bits (244), Expect = 2.750e-23
Identity = 47/60 (78.33%), Postives = 53/60 (88.33%), Query Frame = 1 
Query:  232 TGQCFVVKDNIDTDQIIPAEYLTLVPSNADEYEKLGSYAMIGLPD-VYTTRYVEEGSMAT 408
            TG+CFV KDNIDTDQIIPAEYLTLVPS  DEYEKLGSYA+IGLPD +Y TR+V++G M T
Sbjct:    5 TGKCFVTKDNIDTDQIIPAEYLTLVPSKPDEYEKLGSYALIGLPDDLYKTRFVKDGKMKT 64
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BLAST of mRNA_Ecto-sp13_S_contig11576.1372.1 vs. uniprot
Match: UPI00053C5E2F (3-isopropylmalate dehydratase small subunit 3-like n=1 Tax=Tarenaya hassleriana TaxID=28532 RepID=UPI00053C5E2F)

HSP 1 Score: 99.4 bits (246), Expect = 6.660e-23
Identity = 47/60 (78.33%), Postives = 51/60 (85.00%), Query Frame = 1 
Query:  229 YTGQCFVVKDNIDTDQIIPAEYLTLVPSNADEYEKLGSYAMIGLPDVYTTRYVEEGSMAT 408
            + G C+VV DNIDTDQIIP+EYLTLVPSN DEYEKLGSYAMIGLP  YTTR+VE G M T
Sbjct:   70 FHGLCYVVGDNIDTDQIIPSEYLTLVPSNPDEYEKLGSYAMIGLPASYTTRFVEPGEMKT 129
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig11576.1372.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JPR9_9PHAE1.920e-8296.32Aconitase_C domain-containing protein n=2 Tax=Ecto... [more]
B7ZGN7_9PHAE4.290e-4568.153-isopropylmalate dehydratase (Fragment) n=1 Tax=L... [more]
W7SZS5_9STRA1.720e-2665.823-isopropylmalate dehydratase (Fragment) n=1 Tax=N... [more]
A0A7J7Q700_9CHLO2.400e-2576.92Small subunit of isopropylmalate dehydratase n=1 T... [more]
A0A2J8A327_9CHLO1.010e-2471.013-isopropylmalate dehydratase small subunit n=1 Ta... [more]
A8HYG8_CHLRE3.770e-2478.13Isopropylmalate dehydratase, small subunit n=4 Tax... [more]
A0A4D9CY72_9STRA4.370e-2465.82Uncharacterized protein n=1 Tax=Nannochloropsis sa... [more]
A0A836BR91_9CHLO5.310e-2473.91Aconitase_C domain-containing protein n=1 Tax=Edap... [more]
A6XGU6_9CHLO2.750e-2378.333-isopropylmalate dehydratase (Fragment) n=1 Tax=P... [more]
UPI00053C5E2F6.660e-2378.333-isopropylmalate dehydratase small subunit 3-like... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig11576contigEcto-sp13_S_contig11576:3344..5362 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score249.6
Seed ortholog evalue8.4e-64
Seed eggNOG ortholog2880.D8LRK8
Model size408
KEGG rclassRC00497,RC00976,RC00977,RC01041,RC01046,RC03072
KEGG koko:K01704,ko:K21359
KEGG ReactionR03896,R03898,R03968,R04001,R08620,R08624,R08628,R08634,R08641,R08645,R10170
KEGG Pathwayko00290,ko00660,ko00966,ko01100,ko01110,ko01210,ko01230,map00290,map00660,map00966,map01100,map01110,map01210,map01230
KEGG ModuleM00432,M00535
Hectar predicted targeting categorychloroplast
GOsGO:0003674,GO:0003824,GO:0003861,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006082,GO:0006520,GO:0006551,GO:0006790,GO:0006807,GO:0006950,GO:0006970,GO:0006996,GO:0007275,GO:0008150,GO:0008152,GO:0008652,GO:0009058,GO:0009081,GO:0009082,GO:0009098,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0009628,GO:0009651,GO:0009657,GO:0009658,GO:0009987,GO:0016043,GO:0016053,GO:0016143,GO:0016144,GO:0016829,GO:0016835,GO:0016836,GO:0019748,GO:0019752,GO:0019757,GO:0019758,GO:0019760,GO:0019761,GO:0032501,GO:0032502,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0044237,GO:0044238,GO:0044249,GO:0044272,GO:0044281,GO:0044283,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0044550,GO:0046394,GO:0048229,GO:0048856,GO:0050896,GO:0071704,GO:0071840,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901607,GO:1901657,GO:1901659
Exons4
EggNOG free text desc.3-isopropylmalate dehydratase activity
EggNOG OGsCOG0065@1,KOG0454@2759
EC4.2.1.170,4.2.1.33,4.2.1.35
Cds size408
COG Functional cat.E
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01601,ko00000,ko00001,ko00002,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462607.9783602-CDS-Ecto-sp13_S_contig11576:3343..34661681462607.9783602-CDS-Ecto-sp13_S_contig11576:3343..3466Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11576 3344..3466 +
1681462607.9929178-CDS-Ecto-sp13_S_contig11576:3662..38541681462607.9929178-CDS-Ecto-sp13_S_contig11576:3662..3854Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11576 3663..3854 +
1681462608.0299873-CDS-Ecto-sp13_S_contig11576:4942..50121681462608.0299873-CDS-Ecto-sp13_S_contig11576:4942..5012Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11576 4943..5012 +
1681462608.0418706-CDS-Ecto-sp13_S_contig11576:5339..53621681462608.0418706-CDS-Ecto-sp13_S_contig11576:5339..5362Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11576 5340..5362 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig11576.1372.1prot_Ecto-sp13_S_contig11576.1372.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig11576 3344..5362 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig11576.1372.1

>prot_Ecto-sp13_S_contig11576.1372.1 ID=prot_Ecto-sp13_S_contig11576.1372.1|Name=mRNA_Ecto-sp13_S_contig11576.1372.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=136bp
MMWGFSTTATMVAAVSSSVSAFVVPMPTAGTTAVASRPGCKSPAGVAPRR
AAEADAAAIDDPRVKEMPKNRDRPDKYTGQCFVVKDNIDTDQIIPAEYLT
LVPSNADEYEKLGSYAMIGLPDVYTTRYVEEGSMAT
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mRNA from alignment at Ecto-sp13_S_contig11576:3344..5362+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig11576.1372.1 ID=mRNA_Ecto-sp13_S_contig11576.1372.1|Name=mRNA_Ecto-sp13_S_contig11576.1372.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2019bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11576:3344..5362+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGATGTGGGGCTTCTCGACGACGGCAACCATGGTGGCGGCAGTATCGTC
AAGCGTGTCGGCATTCGTTGTGCCGATGCCAACAGCGGGGACTACTGCCG
TCGCATCGCGACCTGGTTGCAAGGTATGTAACATCATGTGTTGGGTGCCC
TGCTGTTGAGCAACAACAACCGCACGACCTGCTGCGTGTCCAATAATCAT
CAGCCCAAGCACTGACCCATTCCCACCACCAGCTGTGCTAGCATCCCTTT
TGACCCTTCGTTCTACCTCTGGCTGACGCGCGCCTTGCCTGACTCTGTGA
ACAAAATGCAACAAACAAGTCGCCCGCTGGTGTGGCACCGCGAAGAGCTG
CCGAGGCGGATGCAGCTGCCATCGATGACCCTCGCGTAAAGGAGATGCCC
AAGAACAGGGATCGACCAGACAAGTACACGGGGCAGTGCTTCGTCGTGAA
GGACAACATCGACACCGACCAGATCATCCCGGCGGAGTACCTTACTCTCG
TGCCGTCTAATGTAGGCATAGGTGGCGCATGAATGTCAAGGGTTCGATAG
GATACACATAATACGCTGACTACATGAAGAAAATAGTGCACTCTTTTTGC
TGGGAAGATCTAAAAGTATGTCTGTTGCTGCTCCCGCCGCTCGCTGTACC
TGCGGGCCTGTAGATATGTGTGTGGAGCACGTTGTTTTGAGGTTCAGCAG
TACAGCATCCATACGACAGGCTCAACTTCCCATGCATACTCGAGGTGTTT
TGTAAGCAATTTGGTGTAAGACAATCTACTTAATGCAGCAGTAGAGGGCT
CTGCGTATTGCAGAACAGGGATGTAAGGTTTTAGTACAGGAGTGGACACT
CATGCCAAGAGGTTTTACCGGCACTACTGAGGACCCTCTACTCTGTACAT
TACGCAGTCGCATCCAATATCCCACAGTCACATGAAACATGTGCTTCGAC
AAATTCGGCGTCGTGTATCGCTTCAGCAGCGGAATCGTTATACCCCAGAC
ACGCGCCAGCAAGCAGTGGTGGTCGACTTCGAACCTAACCAGGCCTTCTC
CCAAAGCCAAGAGAGTCAGTAGGTAACGTTTGAGATGCCGCAGACGTCAC
CCCCCTGCCCTGTCCCCTCTGACGACCAACTGCGCCCGCCCTCAGTTATT
CTTTGGCCTCCACTCCCAGCCTTAACCTTCGATCATCGATGCATGAGCTG
CTTAGAATGGCCCCGCTTCCACTCCATCCTATTTCACGGAGCGCAACCTG
TATTAAGCTCTAGACAATCCGTTCTTTCCTTCACCAAACCCTTGTACCGG
ACACGGGCCCAGCTCGATGCTGGTTGATCTTGGTGCTTGACGTCTCCAAT
GACTTAGCGTCGTTATCGGTTGCCAACCGTATGTAGTGTACGGCGGTATT
ATTCCGAGAGGTGTAGGCACCGTGGTAACTTGTACGAAGAGCGCAAGAAT
ACTTTGGTATGGTATATTGTCAACATGGTTTCCGTTTTGTGCCAGCTGCT
AACGGACTGGTTTCGTTTGCTAATGCAACTCCTCTTCAAACCGAGTTAGA
CTACCGCCTGCTGGCGTGTACTGCCTCTGCCTCCTCTTTGAATGTCCAGG
CCGATGAGTACGAGAAGCTGGGTTCATACGCCATGATCGGACTTCCGGAT
GTCTACACTACACGTTATGGTGAGTCAGTGCATCAGGTTCTCCATGACGT
CGAATATGGAGCGGTTGGTGTCATACAGCACCGCTGAGCATGATCTTTTT
CTGATCTTTCCGCTGTAGAACGGAGTGTTCGCGGCTTTCGGGGGTTAGAC
AATCACCGAAAGGCGGCTTCGCGAACCCAGAGAAAAGCAGCGACGGCTCG
CGTCGACAGGTGCAATTTTGTTGCTGAGCAGAAAAAGAAGAACCTTTAGT
AGCACCCGGACGCCACCATCTGAACGCACCAACTGCTGTTTGTGGCCCGC
CCCGCCTCTGTCTCTACACGTACTCCAATTAACACGACATTTGCAGTGGA
GGAGGGGTCTATGGCAACG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig11576:3344..5362+

>mRNA_Ecto-sp13_S_contig11576.1372.1 ID=mRNA_Ecto-sp13_S_contig11576.1372.1|Name=mRNA_Ecto-sp13_S_contig11576.1372.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=408bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11576:3344..5362+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGATGTGGGGCTTCTCGACGACGGCAACCATGGTGGCGGCAGTATCGTC
AAGCGTGTCGGCATTCGTTGTGCCGATGCCAACAGCGGGGACTACTGCCG
TCGCATCGCGACCTGGTTGCAAGTCGCCCGCTGGTGTGGCACCGCGAAGA
GCTGCCGAGGCGGATGCAGCTGCCATCGATGACCCTCGCGTAAAGGAGAT
GCCCAAGAACAGGGATCGACCAGACAAGTACACGGGGCAGTGCTTCGTCG
TGAAGGACAACATCGACACCGACCAGATCATCCCGGCGGAGTACCTTACT
CTCGTGCCGTCTAATGCCGATGAGTACGAGAAGCTGGGTTCATACGCCAT
GATCGGACTTCCGGATGTCTACACTACACGTTATGTGGAGGAGGGGTCTA
TGGCAACG
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