mRNA_Ecto-sp13_S_contig11245.1108.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig11245.1108.1
Unique NamemRNA_Ecto-sp13_S_contig11245.1108.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig11245.1108.1 vs. uniprot
Match: A0A6H5JEA6_9PHAE (ERCC4 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JEA6_9PHAE)

HSP 1 Score: 249 bits (637), Expect = 2.660e-74
Identity = 130/142 (91.55%), Postives = 133/142 (93.66%), Query Frame = 1 
Query:    1 TSFLPAERTAREGKVTRLSTPLAEAGKGGSSGSSRRGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDKVRPFIGWPEVVDE 426
            TSFLPAERTAREGKVTRLSTPLAEAG GGSSGSSRRGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK    +G  ++  E
Sbjct:  952 TSFLPAERTAREGKVTRLSTPLAEAGNGGSSGSSRRGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDKAFCLLGSGDLTSE 1093
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BLAST of mRNA_Ecto-sp13_S_contig11245.1108.1 vs. uniprot
Match: D7FH45_ECTSI (ERCC4 domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FH45_ECTSI)

HSP 1 Score: 243 bits (620), Expect = 4.140e-72
Identity = 126/129 (97.67%), Postives = 127/129 (98.45%), Query Frame = 1 
Query:    1 TSFLPAERTAREGKVTRLSTPLAEAGKGGSSGSSRRGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 387
            TSFLPAERTAREGKVTRLSTPLAEAGKG SSGSSR+GGRRKAQA LRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK
Sbjct:  861 TSFLPAERTAREGKVTRLSTPLAEAGKGSSSGSSRKGGRRKAQASLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 989
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BLAST of mRNA_Ecto-sp13_S_contig11245.1108.1 vs. uniprot
Match: A0A6A0A349_HAELA (DNA repair endonuclease UVH1 (Fragment) n=1 Tax=Haematococcus lacustris TaxID=44745 RepID=A0A6A0A349_HAELA)

HSP 1 Score: 133 bits (335), Expect = 6.910e-38
Identity = 69/99 (69.70%), Postives = 79/99 (79.80%), Query Frame = 1 
Query:  106 RGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDKVRPFI 402
            RGG  KAQ P +VVVD+REF S LP +LHQQGFELVPL I VGDYVL+P +CVERK+L DL  S++SGRLY+Q EAM KHYK PVLLIEF  D  RPF+
Sbjct:    9 RGGAAKAQ-PRKVVVDMREFMSSLPAVLHQQGFELVPLTIEVGDYVLSPTMCVERKALPDLVSSLASGRLYSQAEAMCKHYKTPVLLIEFEGD--RPFL 104
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BLAST of mRNA_Ecto-sp13_S_contig11245.1108.1 vs. uniprot
Match: UPI001F5D44C3 (DNA repair endonuclease UVH1 n=1 Tax=Lolium rigidum TaxID=89674 RepID=UPI001F5D44C3)

HSP 1 Score: 137 bits (344), Expect = 1.140e-34
Identity = 63/106 (59.43%), Postives = 84/106 (79.25%), Query Frame = 1 
Query:  109 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDKVRPFIGWPEVVDE 426
            GGR+  +  ++V+VD+REF S LPN+LHQ+G  ++P+ + VGDYVL+P ICVERKS+SDLFQS +SGRLYNQVE M+++YK+PVLLIEF+ DK   F    EV D+
Sbjct:  747 GGRKPTEKEMQVIVDMREFMSSLPNVLHQKGIRIIPVTLEVGDYVLSPLICVERKSISDLFQSFASGRLYNQVETMIRYYKIPVLLIEFSQDKSFSFQSASEVGDD 852
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BLAST of mRNA_Ecto-sp13_S_contig11245.1108.1 vs. uniprot
Match: A0A3S3NAZ6_9MAGN (DNA repair endonuclease UVH1 n=1 Tax=Cinnamomum micranthum f. kanehirae TaxID=337451 RepID=A0A3S3NAZ6_9MAGN)

HSP 1 Score: 130 bits (327), Expect = 1.730e-34
Identity = 59/106 (55.66%), Postives = 82/106 (77.36%), Query Frame = 1 
Query:  109 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDKVRPFIGWPEVVDE 426
            GGR+ A+   +V+VD+REF S LPN+LHQ+G  ++P+ + VGDYVL+P ICVERKS++DLF S +SGRLYNQVE M ++Y++PVLLIEF+ DK   F    ++ D+
Sbjct:   49 GGRKAAERETQVIVDIREFMSSLPNVLHQKGMRIIPVTLEVGDYVLSPLICVERKSIADLFSSFASGRLYNQVETMARYYRIPVLLIEFSQDKSFSFQSASDIGDD 154
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BLAST of mRNA_Ecto-sp13_S_contig11245.1108.1 vs. uniprot
Match: A0A1Y1I3K2_KLENI (Structure-specific endonuclease ERCC1-XPF n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1I3K2_KLENI)

HSP 1 Score: 136 bits (342), Expect = 2.150e-34
Identity = 63/104 (60.58%), Postives = 84/104 (80.77%), Query Frame = 1 
Query:   76 GKGGSSGSSRRGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 387
            G GG+  S+R+ G R+    ++VVVD+REF S LP++LHQQG ++VP+ + VGDY+L+P++CVERKSL+DLFQS  SGRLYNQ EAM ++YK PVLLIEF+ DK
Sbjct:  955 GPGGTGISTRKAGGRQKDRAMQVVVDMREFNSSLPSVLHQQGMKIVPVTLEVGDYILSPEVCVERKSLADLFQSFGSGRLYNQAEAMTRYYKTPVLLIEFDQDK 1058
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BLAST of mRNA_Ecto-sp13_S_contig11245.1108.1 vs. uniprot
Match: A0A8J4VB27_9ROSI (ERCC4 domain-containing protein n=1 Tax=Castanea mollissima TaxID=60419 RepID=A0A8J4VB27_9ROSI)

HSP 1 Score: 129 bits (324), Expect = 3.510e-34
Identity = 56/106 (52.83%), Postives = 83/106 (78.30%), Query Frame = 1 
Query:  109 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDKVRPFIGWPEVVDE 426
            GGR++ +  ++V+VD+REF S LPN+LHQ+G  ++P+ + VGDY+++P ICVERKS+ DLF S +SGRLY+QVE M+++YK+PVLLIEF+ DK   F    ++ D+
Sbjct:   35 GGRKEIEREMQVIVDMREFMSSLPNILHQRGMHIIPVTLEVGDYIISPLICVERKSIQDLFMSFTSGRLYHQVETMVRYYKIPVLLIEFSQDKSFSFQSASDIGDD 140
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BLAST of mRNA_Ecto-sp13_S_contig11245.1108.1 vs. uniprot
Match: UPI000B8CC7E5 (DNA repair endonuclease UVH1-like isoform X3 n=1 Tax=Carica papaya TaxID=3649 RepID=UPI000B8CC7E5)

HSP 1 Score: 127 bits (319), Expect = 4.900e-34
Identity = 55/106 (51.89%), Postives = 84/106 (79.25%), Query Frame = 1 
Query:  109 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDKVRPFIGWPEVVDE 426
            GGR++A+  ++V+VD+REF S LPN+LH +G  ++P+ + VGDY+L+P +CVERKS+ DLF S +SGRLY+QVE+M+++Y++PVLLIEF+ DK   F    ++ D+
Sbjct:   41 GGRKEAEKEMQVIVDVREFMSSLPNMLHLKGMRIIPVTLEVGDYILSPLLCVERKSIQDLFSSFTSGRLYHQVESMVRYYRIPVLLIEFSQDKSFSFQSASDIGDD 146
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BLAST of mRNA_Ecto-sp13_S_contig11245.1108.1 vs. uniprot
Match: A0A5J9WL85_9POAL (ERCC4 domain-containing protein n=1 Tax=Eragrostis curvula TaxID=38414 RepID=A0A5J9WL85_9POAL)

HSP 1 Score: 135 bits (339), Expect = 5.360e-34
Identity = 62/106 (58.49%), Postives = 84/106 (79.25%), Query Frame = 1 
Query:  109 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDKVRPFIGWPEVVDE 426
            GGR+     ++V+VD+REF S LPN+LHQ+G ++VP+ + VGDYVL+P ICVERKS++DLFQS +SGRLYNQVE M+++YK+PVLLIEF+ DK   F    E+ D+
Sbjct:  750 GGRKAPDKEMQVIVDMREFMSSLPNVLHQKGIQIVPVTLEVGDYVLSPLICVERKSIADLFQSFASGRLYNQVETMVRYYKIPVLLIEFSQDKSFSFQSASEIGDD 855
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BLAST of mRNA_Ecto-sp13_S_contig11245.1108.1 vs. uniprot
Match: A0A8J5W2E0_ZIZPA (ERCC4 domain-containing protein (Fragment) n=1 Tax=Zizania palustris TaxID=103762 RepID=A0A8J5W2E0_ZIZPA)

HSP 1 Score: 135 bits (339), Expect = 5.590e-34
Identity = 59/106 (55.66%), Postives = 85/106 (80.19%), Query Frame = 1 
Query:  109 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDKVRPFIGWPEVVDE 426
            GGR+ ++  ++V+VD+REF S LPN+LHQ+G  ++P+ + +GDYVL+P ICVERKS++DLFQS +SGRLYNQ+E M+++YK+PVLLIEF+ DK   F    E+ D+
Sbjct:  582 GGRKPSEKEMQVIVDMREFMSSLPNVLHQKGIRIIPITLEIGDYVLSPLICVERKSIADLFQSFASGRLYNQIETMVRYYKIPVLLIEFSQDKSFSFQSASEIGDD 687
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig11245.1108.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JEA6_9PHAE2.660e-7491.55ERCC4 domain-containing protein n=1 Tax=Ectocarpus... [more]
D7FH45_ECTSI4.140e-7297.67ERCC4 domain-containing protein n=1 Tax=Ectocarpus... [more]
A0A6A0A349_HAELA6.910e-3869.70DNA repair endonuclease UVH1 (Fragment) n=1 Tax=Ha... [more]
UPI001F5D44C31.140e-3459.43DNA repair endonuclease UVH1 n=1 Tax=Lolium rigidu... [more]
A0A3S3NAZ6_9MAGN1.730e-3455.66DNA repair endonuclease UVH1 n=1 Tax=Cinnamomum mi... [more]
A0A1Y1I3K2_KLENI2.150e-3460.58Structure-specific endonuclease ERCC1-XPF n=1 Tax=... [more]
A0A8J4VB27_9ROSI3.510e-3452.83ERCC4 domain-containing protein n=1 Tax=Castanea m... [more]
UPI000B8CC7E54.900e-3451.89DNA repair endonuclease UVH1-like isoform X3 n=1 T... [more]
A0A5J9WL85_9POAL5.360e-3458.49ERCC4 domain-containing protein n=1 Tax=Eragrostis... [more]
A0A8J5W2E0_ZIZPA5.590e-3455.66ERCC4 domain-containing protein (Fragment) n=1 Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig11245contigEcto-sp13_S_contig11245:4109..5339 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score244.2
Seed ortholog evalue3.7e-62
Seed eggNOG ortholog2880.D7FH45
Preferred nameRAD1
Model size426
KEGG koko:K10848
KEGG Pathwayko03420,ko03460,map03420,map03460
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0000014,GO:0000109,GO:0000110,GO:0000280,GO:0000710,GO:0000712,GO:0000724,GO:0000725,GO:0000726,GO:0000735,GO:0000736,GO:0003006,GO:0003674,GO:0003676,GO:0003677,GO:0003684,GO:0003697,GO:0003824,GO:0004518,GO:0004519,GO:0004520,GO:0004536,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0006139,GO:0006259,GO:0006277,GO:0006281,GO:0006289,GO:0006296,GO:0006298,GO:0006302,GO:0006310,GO:0006312,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0006979,GO:0006996,GO:0007049,GO:0007059,GO:0007127,GO:0007131,GO:0007530,GO:0007531,GO:0007533,GO:0007534,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009314,GO:0009628,GO:0009987,GO:0016043,GO:0016787,GO:0016788,GO:0018130,GO:0019438,GO:0022402,GO:0022413,GO:0022414,GO:0030154,GO:0032502,GO:0032505,GO:0032991,GO:0033554,GO:0033683,GO:0034641,GO:0034645,GO:0034654,GO:0035822,GO:0035825,GO:0036297,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0045002,GO:0045132,GO:0045165,GO:0046483,GO:0048285,GO:0048869,GO:0050896,GO:0051304,GO:0051307,GO:0051321,GO:0051716,GO:0061982,GO:0071704,GO:0071840,GO:0071897,GO:0090304,GO:0090305,GO:0097159,GO:0098813,GO:0140013,GO:0140097,GO:1901255,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1903046,GO:1990391,GO:1990599
Exons3
EggNOG free text desc.resolution of meiotic recombination intermediates
EggNOG OGsCOG1948@1,KOG0442@2759
Cds size426
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03032,ko03400
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462587.407655-CDS-Ecto-sp13_S_contig11245:4108..42101681462587.407655-CDS-Ecto-sp13_S_contig11245:4108..4210Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11245 4109..4210 -
1681462587.4195688-CDS-Ecto-sp13_S_contig11245:4850..49791681462587.4195688-CDS-Ecto-sp13_S_contig11245:4850..4979Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11245 4851..4979 -
1681462587.4320574-CDS-Ecto-sp13_S_contig11245:5144..53391681462587.4320574-CDS-Ecto-sp13_S_contig11245:5144..5339Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11245 5145..5339 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig11245.1108.1prot_Ecto-sp13_S_contig11245.1108.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig11245 4109..5339 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig11245.1108.1

>prot_Ecto-sp13_S_contig11245.1108.1 ID=prot_Ecto-sp13_S_contig11245.1108.1|Name=mRNA_Ecto-sp13_S_contig11245.1108.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=142bp
TSFLPAERTAREGKVTRLSTPLAEAGKGGSSGSSRRGGRRKAQAPLRVVV
DLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSM
SSGRLYNQVEAMLKHYKVPVLLIEFNPDKVRPFIGWPEVVDE
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mRNA from alignment at Ecto-sp13_S_contig11245:4109..5339-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig11245.1108.1 ID=mRNA_Ecto-sp13_S_contig11245.1108.1|Name=mRNA_Ecto-sp13_S_contig11245.1108.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1231bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11245:4109..5339- (Ectocarpus species13 EcNAP12_S_4_19m)
ACCTCGTTTTTACCAGCGGAACGGACCGCCCGAGAAGGAAAAGTGACACG
GCTTTCTACACCCCTAGCGGAGGCTGGAAAGGGTGGCAGCAGCGGCAGCA
GCAGAAGGGGAGGACGTCGGAAGGCGCAAGCGCCTCTCAGGGTAGTCGTG
GACCTTCGAGAGTTCCGCAGCGTCCTTCCTAACCTGCTGCATCAGGTAGA
ATCCGATACGGCGGTTTCACTGTTCCTTTGTTTTTTTGCCTCCATGTTGG
GTCACACACATGTCGCCCTTGTCCACATCTCCATCCGGTCAATGGTCTAA
GCCCTACATGAGGCATGCAAAACATGAGTACGCCCCTCCGCGACCCCCCC
CGCTCTTCAGCAAGGGTTTGAGCTGGTCCCGTTGGTCATAGCGGTCGGCG
ACTACGTCCTGACGCCCCAGATCTGCGTCGAGCGAAAGAGCTTGTCCGAC
TTGTTCCAGTCCATGTCTTCCGGCAGACTGTACAACCAGGTTTGTTTCGT
TGTTTTGGTGGAGAGTGCTGGCCATTGGCTGAGGAATCACCATTCCTGCC
GGTGGTTGATGTTTTGGTTGCTGGTGGTTGGTGTAGCCCTCATCGTTACC
GTTGACTTTGTGCCGTGGCCCAGATGCCGTTGTTGTTGATGATGTTGTTG
TTCCTCTAACTTACGCGTATGTGAAAGTCAAGAACCGGCCGAGAGTGACG
GTGCCGATTTGATGCCCGTGGGTGGGTGGGGGCAGTCAAATTTCGTGTGG
TACTTGTTGCTGCAAGTATACCGTCTCTTTTTTTTGTTTGGTTTTTGGAA
ATGGCGTGTTTTTCTCCATTGTTCACCACCTAGGCTGGGGCATTCAGAAG
AGTGCCGAAAGGTTTAAGGACAGAACGGTCGAAAGGTGTACGGACAGAAC
AGTAGCGAAAGGGGTAAGGATCGACGTCCAAAAGAAAAAGGGATGGTACC
GCCGTTGTTTCGTTCGACTGTTTGTTTAAGAGATGACGACAAACTAATCT
GCAAAAGCAAAGCAGTCTCGTTGCGGGTTTCTGTGAGTAGTTTGATTTTG
TGTTTTGTGGGGAAGGCATGTGTCACGAAACGGGTGTCGTAGATGCCTTC
GTCGAATCGCGCTTTTGTCATGTCCTCAGGTGGAGGCGATGCTGAAGCAC
TACAAGGTGCCCGTCTTGCTTATCGAGTTCAATCCTGACAAGGTGCGTCC
ATTCATTGGTTGGCCTGAAGTTGTTGATGAA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig11245:4109..5339-

>mRNA_Ecto-sp13_S_contig11245.1108.1 ID=mRNA_Ecto-sp13_S_contig11245.1108.1|Name=mRNA_Ecto-sp13_S_contig11245.1108.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=426bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11245:4109..5339- (Ectocarpus species13 EcNAP12_S_4_19m)
ACCTCGTTTTTACCAGCGGAACGGACCGCCCGAGAAGGAAAAGTGACACG
GCTTTCTACACCCCTAGCGGAGGCTGGAAAGGGTGGCAGCAGCGGCAGCA
GCAGAAGGGGAGGACGTCGGAAGGCGCAAGCGCCTCTCAGGGTAGTCGTG
GACCTTCGAGAGTTCCGCAGCGTCCTTCCTAACCTGCTGCATCAGCAAGG
GTTTGAGCTGGTCCCGTTGGTCATAGCGGTCGGCGACTACGTCCTGACGC
CCCAGATCTGCGTCGAGCGAAAGAGCTTGTCCGACTTGTTCCAGTCCATG
TCTTCCGGCAGACTGTACAACCAGGTGGAGGCGATGCTGAAGCACTACAA
GGTGCCCGTCTTGCTTATCGAGTTCAATCCTGACAAGGTGCGTCCATTCA
TTGGTTGGCCTGAAGTTGTTGATGAA
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