mRNA_E-siliculosus-1a_F_contig1050.365.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig1050.365.1
Unique NamemRNA_E-siliculosus-1a_F_contig1050.365.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig1050.365.1 vs. uniprot
Match: D7FZB9_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FZB9_ECTSI)

HSP 1 Score: 140 bits (352), Expect = 1.730e-37
Identity = 66/67 (98.51%), Postives = 67/67 (100.00%), Query Frame = 1 
Query:   43 MGRDCKEAARALYACMKKTQCMKDGGRLKECLKTSDREFCRQDHQSFFECKRGQLDMRTRIRGERSF 243
            MGRDCKEAARALYACMKKTQCMKDGGRLK+CLKTSDREFCRQDHQSFFECKRGQLDMRTRIRGERSF
Sbjct:    1 MGRDCKEAARALYACMKKTQCMKDGGRLKDCLKTSDREFCRQDHQSFFECKRGQLDMRTRIRGERSF 67
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BLAST of mRNA_E-siliculosus-1a_F_contig1050.365.1 vs. uniprot
Match: A0A835ZA98_9STRA (Cytochrome c oxidase assembly protein PET191 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZA98_9STRA)

HSP 1 Score: 89.7 bits (221), Expect = 1.060e-18
Identity = 41/67 (61.19%), Postives = 53/67 (79.10%), Query Frame = 1 
Query:   43 MGRDCKEAARALYACMKKTQCMKDGGRLKECLKTSDREFCRQDHQSFFECKRGQLDMRTRIRGERSF 243
            M  DCKE ARAL ACM++++CM+ GG+L+ECLKT +   C    +S+FEC+RGQLDMRTRIRG RS+
Sbjct:    1 MSSDCKEHARALLACMQESKCMQGGGKLRECLKTDEVHQCELQRRSYFECRRGQLDMRTRIRGPRSY 67
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BLAST of mRNA_E-siliculosus-1a_F_contig1050.365.1 vs. uniprot
Match: A0A482S5P5_9ARCH (Uncharacterized protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482S5P5_9ARCH)

HSP 1 Score: 71.6 bits (174), Expect = 3.280e-12
Identity = 35/68 (51.47%), Postives = 48/68 (70.59%), Query Frame = 1 
Query:   43 MGRDCKEAARALYACMKKTQCMKDGGRLKECLKTSDREF-CRQDHQSFFECKRGQLDMRTRIRGERSF 243
            MG+ CK+ AR L  CM +TQC++ G  +KEC+KT + E  C Q  +++FECKRG LDMR RIRG + +
Sbjct:    1 MGKSCKDIARTLLDCMAETQCVQQGQDVKECMKTMEGEDRCGQLRRAYFECKRGSLDMRYRIRGPKVY 68
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BLAST of mRNA_E-siliculosus-1a_F_contig1050.365.1 vs. uniprot
Match: K3X2Q5_GLOUD (Uncharacterized protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3X2Q5_GLOUD)

HSP 1 Score: 70.1 bits (170), Expect = 1.540e-11
Identity = 37/65 (56.92%), Postives = 45/65 (69.23%), Query Frame = 1 
Query:   43 MGRDCKEAARALYACMKKTQCMKDGGR-LKECLKTSDREF-CRQDHQSFFECKRGQLDMRTRIRG 231
            MG+ C++ A AL  CM   +CM DG R LK+CLK  +    CR   Q++FECKRGQLDMRTRIRG
Sbjct:    1 MGKSCRDMAEALRDCMVTEECMSDGTRTLKQCLKMKEYAHECRGYRQAYFECKRGQLDMRTRIRG 65
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BLAST of mRNA_E-siliculosus-1a_F_contig1050.365.1 vs. uniprot
Match: A0A3R7MTX6_9STRA (Uncharacterized protein n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3R7MTX6_9STRA)

HSP 1 Score: 67.8 bits (164), Expect = 9.360e-11
Identity = 35/65 (53.85%), Postives = 46/65 (70.77%), Query Frame = 1 
Query:   43 MGRDCKEAARALYACMKKTQCMKDGGR-LKECLKTSDREF-CRQDHQSFFECKRGQLDMRTRIRG 231
            MG+ C++AA AL  CM + +CM DG R LK+CL      + C++   ++FECKRGQLDMRTRIRG
Sbjct:    1 MGKACRDAAEALRDCMMEQECMADGTRTLKQCLHERTYSYECKEFRVAYFECKRGQLDMRTRIRG 65
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BLAST of mRNA_E-siliculosus-1a_F_contig1050.365.1 vs. uniprot
Match: A0A2D4CB04_PYTIN (Uncharacterized protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4CB04_PYTIN)

HSP 1 Score: 66.6 bits (161), Expect = 2.530e-10
Identity = 34/65 (52.31%), Postives = 45/65 (69.23%), Query Frame = 1 
Query:   43 MGRDCKEAARALYACMKKTQCMKDGGR-LKECLKTSDREF-CRQDHQSFFECKRGQLDMRTRIRG 231
            MG+ C++ A  L  CM + +CM DG R LK+CL+  +    CR+   ++FECKRGQLDMRTRIRG
Sbjct:    1 MGKSCRDMAELLRDCMVEEECMSDGTRTLKQCLRMKEYAHECRELRYAYFECKRGQLDMRTRIRG 65
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BLAST of mRNA_E-siliculosus-1a_F_contig1050.365.1 vs. uniprot
Match: A0A8K1CG31_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CG31_PYTOL)

HSP 1 Score: 66.2 bits (160), Expect = 3.360e-10
Identity = 36/67 (53.73%), Postives = 47/67 (70.15%), Query Frame = 1 
Query:   43 MGRDCKEAARALYACMKKTQCMKDGGR-LKECLKTSDREF---CRQDHQSFFECKRGQLDMRTRIRG 231
            MG+ C++ A  L  CM + +CM+DG R LK+CLK   RE+   C +   ++FECKRGQLDMRTRIRG
Sbjct:    1 MGKSCRDMAELLRDCMVEQECMRDGTRTLKQCLKM--REYAHECSELRYAYFECKRGQLDMRTRIRG 65
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BLAST of mRNA_E-siliculosus-1a_F_contig1050.365.1 vs. uniprot
Match: A0A024TWV1_9STRA (Uncharacterized protein n=3 Tax=Aphanomyces TaxID=100860 RepID=A0A024TWV1_9STRA)

HSP 1 Score: 65.5 bits (158), Expect = 5.670e-10
Identity = 33/65 (50.77%), Postives = 46/65 (70.77%), Query Frame = 1 
Query:   43 MGRDCKEAARALYACMKKTQCMKDGGR-LKECLKTSD-REFCRQDHQSFFECKRGQLDMRTRIRG 231
            MG+ C++ A AL  CM + +CM DG + LKECL+  + R  C++   ++FECKRGQ+DMR RIRG
Sbjct:    1 MGKACRDMAEALRDCMCEKECMSDGTKTLKECLRMEEFRHECKEYRLAYFECKRGQIDMRQRIRG 65
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BLAST of mRNA_E-siliculosus-1a_F_contig1050.365.1 vs. uniprot
Match: A0A6G0XTT3_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XTT3_9STRA)

HSP 1 Score: 65.1 bits (157), Expect = 7.750e-10
Identity = 33/65 (50.77%), Postives = 45/65 (69.23%), Query Frame = 1 
Query:   43 MGRDCKEAARALYACMKKTQCMKDGGR-LKECLKTSD-REFCRQDHQSFFECKRGQLDMRTRIRG 231
            MG+ C++ A AL  CM + +CM DG + LK+CL+  + R  C +   ++FECKRGQLDMR RIRG
Sbjct:    1 MGKSCRDMAEALRDCMVEKECMSDGTKTLKQCLQLEEFRHECHEYRVAYFECKRGQLDMRQRIRG 65
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BLAST of mRNA_E-siliculosus-1a_F_contig1050.365.1 vs. uniprot
Match: A0A067BWW3_SAPPC (Uncharacterized protein n=3 Tax=Saprolegniaceae TaxID=4764 RepID=A0A067BWW3_SAPPC)

HSP 1 Score: 63.9 bits (154), Expect = 1.980e-9
Identity = 32/65 (49.23%), Postives = 44/65 (67.69%), Query Frame = 1 
Query:   43 MGRDCKEAARALYACMKKTQCMKDGGR-LKECLKTSD-REFCRQDHQSFFECKRGQLDMRTRIRG 231
            MG+ C++ A  L  CM + +CM DG R LK CLK  + +  C++   ++FECKRGQ+DMR RIRG
Sbjct:    1 MGKSCRDMAEQLRDCMLEMECMSDGKRTLKTCLKLDEYKHECKEYRYAYFECKRGQIDMRQRIRG 65
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig1050.365.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FZB9_ECTSI1.730e-3798.51Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A835ZA98_9STRA1.060e-1861.19Cytochrome c oxidase assembly protein PET191 n=1 T... [more]
A0A482S5P5_9ARCH3.280e-1251.47Uncharacterized protein n=1 Tax=archaeon TaxID=190... [more]
K3X2Q5_GLOUD1.540e-1156.92Uncharacterized protein n=1 Tax=Globisporangium ul... [more]
A0A3R7MTX6_9STRA9.360e-1153.85Uncharacterized protein n=3 Tax=Phytophthora kerno... [more]
A0A2D4CB04_PYTIN2.530e-1052.31Uncharacterized protein n=1 Tax=Pythium insidiosum... [more]
A0A8K1CG31_PYTOL3.360e-1053.73Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A024TWV1_9STRA5.670e-1050.77Uncharacterized protein n=3 Tax=Aphanomyces TaxID=... [more]
A0A6G0XTT3_9STRA7.750e-1050.77Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
A0A067BWW3_SAPPC1.980e-949.23Uncharacterized protein n=3 Tax=Saprolegniaceae Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig1050contigE-siliculosus-1a_F_contig1050:31138..33374 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start1
Stop1
Cds size204
Model size1292
Exons3
Ec32 orthologEc-07_001660.1
Ec32 ortholog descriptionCytochrome c oxidase assembly protein PET191
Hectar predicted targeting categoryother localisation
BRITEko00000,ko00001,ko00002,ko01000,ko01003,ko03029
Best eggNOG OGNA|NA|NA
Best tax levelPythiales
CAZyGT54
COG Functional cat.S
EC2.4.1.145
EggNOG OGs1MHXH@121069,KOG4114@1,KOG4114@2759
EggNOG free text desc.cytochrome c oxidase assembly protein. Source PGD
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005758,GO:0006996,GO:0007005,GO:0008150,GO:0008535,GO:0009987,GO:0016020,GO:0016021,GO:0016043,GO:0017004,GO:0019866,GO:0022607,GO:0031090,GO:0031224,GO:0031300,GO:0031301,GO:0031304,GO:0031305,GO:0031966,GO:0031967,GO:0031970,GO:0031974,GO:0031975,GO:0032592,GO:0033108,GO:0033617,GO:0034622,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043933,GO:0044085,GO:0044422,GO:0044424,GO:0044425,GO:0044429,GO:0044444,GO:0044446,GO:0044455,GO:0044464,GO:0065003,GO:0070013,GO:0071840,GO:0098573
KEGG ModuleM00075
KEGG Pathwayko00510,ko00513,ko01100,ko04714,map00510,map00513,map01100,map04714
KEGG ReactionR05987
KEGG koko:K00738,ko:K18178
Preferred nameCOA5
Seed eggNOG ortholog36331.EPrPI00000017314
Seed ortholog evalue5.9e-10
Seed ortholog score69.7
Taxonomic scopeEukaryota
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig1050.365.1prot_E-siliculosus-1a_F_contig1050.365.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig1050 31180..32328 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927552.8593426-UTR-E-siliculosus-1a_F_contig1050:31137..311791622927552.8593426-UTR-E-siliculosus-1a_F_contig1050:31137..31179Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1050 31138..31179 +
1696318582.33175-UTR-E-siliculosus-1a_F_contig1050:31137..311791696318582.33175-UTR-E-siliculosus-1a_F_contig1050:31137..31179Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1050 31138..31179 +
1622927552.9219892-UTR-E-siliculosus-1a_F_contig1050:32328..333741622927552.9219892-UTR-E-siliculosus-1a_F_contig1050:32328..33374Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1050 32329..33374 +
1696318582.3698926-UTR-E-siliculosus-1a_F_contig1050:32328..333741696318582.3698926-UTR-E-siliculosus-1a_F_contig1050:32328..33374Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1050 32329..33374 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927552.8757164-CDS-E-siliculosus-1a_F_contig1050:31179..312481622927552.8757164-CDS-E-siliculosus-1a_F_contig1050:31179..31248Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1050 31180..31248 +
1696318582.3417332-CDS-E-siliculosus-1a_F_contig1050:31179..312481696318582.3417332-CDS-E-siliculosus-1a_F_contig1050:31179..31248Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1050 31180..31248 +
1622927552.891107-CDS-E-siliculosus-1a_F_contig1050:31914..319551622927552.891107-CDS-E-siliculosus-1a_F_contig1050:31914..31955Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1050 31915..31955 +
1696318582.3520098-CDS-E-siliculosus-1a_F_contig1050:31914..319551696318582.3520098-CDS-E-siliculosus-1a_F_contig1050:31914..31955Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1050 31915..31955 +
1622927552.905215-CDS-E-siliculosus-1a_F_contig1050:32234..323281622927552.905215-CDS-E-siliculosus-1a_F_contig1050:32234..32328Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1050 32235..32328 +
1696318582.3623626-CDS-E-siliculosus-1a_F_contig1050:32234..323281696318582.3623626-CDS-E-siliculosus-1a_F_contig1050:32234..32328Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1050 32235..32328 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig1050.365.1

>prot_E-siliculosus-1a_F_contig1050.365.1 ID=prot_E-siliculosus-1a_F_contig1050.365.1|Name=mRNA_E-siliculosus-1a_F_contig1050.365.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=68bp
MGRDCKEAARALYACMKKTQCMKDGGRLKECLKTSDREFCRQDHQSFFEC
KRGQLDMRTRIRGERSF*
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mRNA from alignment at E-siliculosus-1a_F_contig1050:31138..33374+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig1050.365.1 ID=mRNA_E-siliculosus-1a_F_contig1050.365.1|Name=mRNA_E-siliculosus-1a_F_contig1050.365.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=2237bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1050:31138..33374+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATTCCACAGGGAAGTGTTTTTGCTCCGGGCGACAAGCAAACGATGGGGAG
GGATTGCAAGGAGGCCGCGCGGGCTCTTTACGCCTGCATGAAAAAGACTC
AGTGCATGAAGGTAGGCACACACAACGTAGCTGTTCACCCCAGTGGCACA
CCACGTACCCTATGTCACATCGCGCCCATGACTCGGCACTGATGAGTACT
CCAGTGTTGTCGCAGGAGGCCGGTGTATGTTGTGTACTCGACAGCCGAAT
CTATTTCGATCTGATACCTGGTTGCGTTCGGAAAAGAGCGACGGTCCACC
TTCGTTGGGTGTGCTGTGCTTTGCCATAAATGCCCGTACCTGCTCTGCTC
TGCGGTATTTTCCCCTATTGGATCCCTTCAACGCTTGGGCATTTCCTGGA
CGACAAGTCATGGCGACATCTGCTGGGTCAGATGGCAGCGATCTTATGAT
GGATTGGAAGAGAAGAAAGCACGCATGTCGAAGAATCGGTAGCTATACAT
TAGACGAAGGAAAGTACGGCGAGCAGTTAGTTCGGCTGACTGCTGTGATT
TCGCGCAGGCAGTTTGCGTGCTGGTGCTGCTTCACCCATCCTCTAAATAC
GCAGGCTGTGTGGTCTTAAACACCAGTATCGATCTCGCATTTCTCAAGCC
CTTCCTCGAAGCCAGGGTGCTCTGCCTTCCCCAGGTATGTGATGAAGAAT
GCGCCCGTCTCGCTCGCTCGCTCCACCCGCCTCCCCCTCTCTCGCGTGGC
GCCTCCTCTTCCGTCGTCTGTCCACAGGATGGTGGCCGCCTGAAGGAGTG
CCTTAAAACATCCGACAGGTTTGTGGCGAATGCTCGATGGGGAGGGGGGG
TGCTGCTTTTGTAGGCCATCCAACGCCGGTTGCGACTTTCTAGTTTAATC
TATGTACCACGTTAGAGGGCAGTGTGGATTTCTTCGTGTCTGTCCAGTGC
GACCCCGTCGTGGCGTTGACTGGCCTTTCGTGCACGTCCACCCATTTTCA
AGCTTTCACGGAGAGGGGATTTGGTGCTCCAAGTGACTCCCCACCGAGAC
TATTTCCCGTTCAAATCTCGTGCTCATTCGTGTCGACAAAACCGCAGGGA
GTTCTGCAGACAGGATCACCAGTCGTTTTTTGAGTGCAAGCGCGGTCAGC
TGGACATGAGGACAAGAATACGGGGAGAGCGGTCCTTCTGACACGCGCTC
GGGCGAGGGTTGGGCAGCAAGCGAAAGCTAGATTCCGGGTTCTCTCTCGA
ACACGCGTTGCGACGCACGAAGGGCCCCGGGGTGTTTCTCACCTTCATGA
GACATGCCTCTGTGTGAAGCAGGCATTTTTCCAGTGGGGGGGTTGTGAGT
CGTTGATGCCGGCAGGAATATGGTTGGAGGGTCTCCGGTGGCCGTGTTTT
TTTCTTTCGCGACTTGGGCAACGCGTTGTTGTTGCCCTTTTGTTGGAGTC
CGGTGCTGGTGACCATCTTGTAGACCTCTTCGCAGGTGCAATTAGCTGGT
TGCGAGGGAACTGCTGTGGCGGGACGTATGCATGTCGTTTTTTTACAATA
GCGCTTGAGGTACCAGGATAGCGGCATCGGGGTGTGGCACTCGAATAGAA
CGAGAGAACAGCCGTGGCGCTATAAAATACACGTATTTCCTATGCCTTAA
ATTTCGAGCCCCTCACACATTTGTAGGTATGCATACATGTGGGCAAAATG
GAGTGGTCGGTGTGACCCGGCAGTGAACACGCCTAGACCGTTGTGGGCAA
AGGTTGTGATCGTATGATTGGTGATCGTTCACGTTGTTTTTTTCTCGCTT
TTCCCGCTTCGCCTCACGCGTGTCGAGTGGGAACCGAGTGGGAACCGCTG
GCTCTGCGTATGGGTCTCCTTTGTCCGAATTCTGACACTATCTTTCACGT
GATGCCACAGTAGGCTGGAGACCTTTTCTGGATTCTTTTTTTTTTCCGCG
GTTATGGTTCATTTCAGTGCGTACAATACTCTTTTAATTTTTCGTCCTGC
TCTTTCTGTAGTGTTTTCATACCATGAGGCATCATGAGATATCACGATGT
ATCATCAGGTGCCGTGAGGTACCATGAGGTACCATGACGTGTCATGATGC
CTCATTAGAGTTCATGTGGTACCATGGAGTATCATTACCAGCCATGAGAT
CCCATGTGGTACCATCAGGTGTCATGAGGTAACATGAGGTACCTGGGGTT
TCGTCAGCGGTCTTAATGTATCATGAGCTATTACGAG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig1050:31138..33374+

>mRNA_E-siliculosus-1a_F_contig1050.365.1 ID=mRNA_E-siliculosus-1a_F_contig1050.365.1|Name=mRNA_E-siliculosus-1a_F_contig1050.365.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=408bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1050:31138..33374+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGGGGAGGGATTGCAAGGAGGCCGCGCGGGCTCTTTACGCCTGCATGAA
AAAGACTCAGTGCATGAAGATGGGGAGGGATTGCAAGGAGGCCGCGCGGG
CTCTTTACGCCTGCATGAAAAAGACTCAGTGCATGAAGGATGGTGGCCGC
CTGAAGGAGTGCCTTAAAACATCCGACAGGATGGTGGCCGCCTGAAGGAG
TGCCTTAAAACATCCGACAGGGAGTTCTGCAGACAGGATCACCAGTCGTT
TTTTGAGTGCAAGCGCGGTCAGCTGGACATGAGGACAAGAATACGGGGAG
AGCGGTCCTTCTGAGGAGTTCTGCAGACAGGATCACCAGTCGTTTTTTGA
GTGCAAGCGCGGTCAGCTGGACATGAGGACAAGAATACGGGGAGAGCGGT
CCTTCTGA
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