prot_E-siliculosus-1a_M_contig8.16191.1 (polypeptide) Ectocarpus siliculosus Ec864m_EcPH12_78m male

BLAST of mRNA_E-siliculosus-1a_M_contig8.16191.1 vs. uniprot
Match: D7FNY6_ECTSI (SH2 domain containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FNY6_ECTSI)

HSP 1 Score: 1734 bits (4492), Expect = 0.000e+0
Identity = 930/1096 (84.85%), Postives = 970/1096 (88.50%), Query Frame = 0

Query:    1 MVPSLPASTRRAHPDTGEMTRLGFIHARPLVRFNDNGTKFWELEPLRTCEELEFVRNTLRGCGRQIKFRAEVANTMNLTRLLNSGCLLLHYAGHGNNEYLAFESTTEWKCGIMEPLKVEYLKNLFLVGEIVKTQLVFMSSCSSEASGNVFVAAGVPHVVAVKHDAGVTDEVAREFATVFYDALFENGGRSTVKQAFDMARNCVNATHLGAPKHEEDLFLLLPRGKXXXXXXXXXARADGNHDVRIFETLPEGKLVDETQRLPPRPPCKKAVSNYMGQARVQHVVRLLLDEKAACVTVTGEPGSGKTELAVQACDYVRDRHHFNSFLWADCDKAALDASPYTSVSSADQFFVFPNSPNGASGDSVSLEDLCRQIGVAIGMPKPGPSTAKKLHDFIFPDTDSGQPFQKVLLVLDNVDSLLERGGDAQDRLVHLLCDLCSMGGGGHLKLLATSEHKLLSGNEGFHGGTEMVTTVERLKTRHASELLMNNSPRDLQPEELGLRSGGLSKPHVVGAVQNHPVLKEVLEIAGGHPGTLVRLAPVLLDGPLDDARTLKEMATKCRADFRDKSYRQRGHRRSGSGTSAGSSS-FAMIAREMSEASSSLGTSAPTSRSPNGSPAPATPSQRPAMRNAFSVDAGHRRDRGPVSLNHTNSSIDGSGXXXXXXXHARPLPPMPHEEDGGEGLPRAYSSSPTGRCGNPRCHGRPQHQHSLRQESGSPPACCLPHQFAQELRDRVAMMYRRRMMAREALEEQPELPQMNMEQQHAWDMAEAAGVERGCRLVWVAATSNMANDRRXXXXXXXXXGWWASKQGEFVSWNCLREGLQRHLHVRMTVPCSSSGGDRHDALEGDHYTYLDDEQEVDSEEVXXXXXXXXXXXXQGRAAGGERRTGNRRMRPLPHATRVLDKPQLDFMRGILRKKQKELANAGETTVDCTEYGLDARIDFISVEAFAKYSRWWAPLMGTLAIIRNDWARTRPVRVHGFIGRLATDEKLLRRARGAFLLRFSERHPGKLVISRAYHHSLVEVTEGGRCYMTVDQQGTRREYGSLHDLVTQNSQLKQLYPNIPKEQVFRGDAPATRPETETEGAREARRENGYELGAAS 1095
            MV SLPASTRRA+PDTGEMTRLGFIHA PLVRF+ N   F EL+ LRTCEELEFVRN LRG GRQIKFRAEVAN MNLTRLL+SGCLLLHYAGHGN+EYLAFESTTEWKCGIMEPLKV+YLKNLF V  IVKTQLVF+SSCSSEASGNVFVAAGVPHVVAVKH+AGVTD  AREFA VFY+ALFENGGRSTVKQAFDMA NCVNATHLGAPKHEEDLFLLLPR              DGNHDVRIF+TLPEGKLVDET RLPPRPPCKKAVS+YMG ARVQHVVRLLLDE+AACVTVTG+PGSGKTELA+QACDYVR RHHFNSFLWADCDKAALDASPY  VSSAD+F+V PNSPNGASGDSVS EDLCRQIGVAIG+P+PGPST  +LH FIFP+TDSGQP QKVLLVLDNVDSLLERGGDAQDRLVHLL DLCSMGGGGHLKLLATSE+KLLSGNE FHGGTE V  VE L+TRHASELL++NSPRDLQPEELGL+SGGLSK HVV A QNHPVLKEVLEIAGGHPGTLVRLAPVLL+GPLDDA  LKEMAT CRADFRDKSYR+RGHRRSGSGTSAGSSS  AMIAREMSEA SSLGTSAPTSRS  GSPAPATPSQRPAM NAFS DAGH RDR  VSLN TNSSIDGS XXXXXXX     PP P EEDGG GLPRA+ S PTGRC NPRCHGRPQ Q SL Q+SGSPP CCL HQFAQELRDRVAMM+RR+MMAREALEEQPELPQMNMEQQHAWDMAEAAGVERGCRL+WV ATSNMAND R    XXXXX   ASK+GEFVSWN LREGLQR+L VRMTVPCSSSGGDRHDALEG  YTYLDD QEVDSEEVXXXXXXXXXXXX  RAAGGERRTGNRRM PLPHATRVLD+ Q  F+RGILRKKQKELANAG  TVDCTEY LD R DFISVE FAK+SRWW PLMGTL IIRNDWARTRPV VHGFIGRL TDE L RRARGAFLLRFSE HPGKLVISRAYHHSLVEVTEGGRCYMTV+QQGTRREY SLHDLVTQN QL+ LY +IP EQVF G APA  PETETEGA EARRENGYELGAAS
Sbjct:    1 MVLSLPASTRRANPDTGEMTRLGFIHAAPLVRFDHNRNMFRELKALRTCEELEFVRNKLRGFGRQIKFRAEVANAMNLTRLLHSGCLLLHYAGHGNDEYLAFESTTEWKCGIMEPLKVKYLKNLFRVDGIVKTQLVFISSCSSEASGNVFVAAGVPHVVAVKHNAGVTDGAAREFANVFYNALFENGGRSTVKQAFDMALNCVNATHLGAPKHEEDLFLLLPR--------------DGNHDVRIFDTLPEGKLVDETHRLPPRPPCKKAVSSYMGPARVQHVVRLLLDERAACVTVTGDPGSGKTELAIQACDYVRYRHHFNSFLWADCDKAALDASPYNLVSSADRFYVLPNSPNGASGDSVSSEDLCRQIGVAIGIPQPGPSTTNELHQFIFPNTDSGQPSQKVLLVLDNVDSLLERGGDAQDRLVHLLGDLCSMGGGGHLKLLATSEYKLLSGNEVFHGGTERVAEVEPLETRHASELLIDNSPRDLQPEELGLQSGGLSKAHVVSAAQNHPVLKEVLEIAGGHPGTLVRLAPVLLNGPLDDACKLKEMATSCRADFRDKSYRRRGHRRSGSGTSAGSSSSLAMIAREMSEAGSSLGTSAPTSRSQCGSPAPATPSQRPAMGNAFSEDAGHGRDRKRVSLNRTNSSIDGSXXXXXXXXXXXXXPPYPDEEDGGGGLPRAHGSGPTGRCRNPRCHGRPQQQPSLGQQSGSPPGCCLRHQFAQELRDRVAMMHRRQMMAREALEEQPELPQMNMEQQHAWDMAEAAGVERGCRLLWVTATSNMANDWRRDNGXXXXXXXXASKRGEFVSWNHLREGLQRNLDVRMTVPCSSSGGDRHDALEGTQYTYLDDGQEVDSEEVXXXXXXXXXXXXXERAAGGERRTGNRRMGPLPHATRVLDRSQWGFIRGILRKKQKELANAGVQTVDCTEYRLDGRYDFISVETFAKFSRWWVPLMGTLGIIRNDWARTRPVFVHGFIGRLETDENL-RRARGAFLLRFSESHPGKLVISRAYHHSLVEVTEGGRCYMTVNQQGTRREYESLHDLVTQNGQLRHLYQDIPMEQVFTGRAPAA-PETETEGAPEARRENGYELGAAS 1080          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16191.1 vs. uniprot
Match: A0A6H5KC67_9PHAE (SH2 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KC67_9PHAE)

HSP 1 Score: 1294 bits (3348), Expect = 0.000e+0
Identity = 745/1070 (69.63%), Postives = 817/1070 (76.36%), Query Frame = 0

Query:   46 LRTCEELEFVRNTLRGCGRQIKFRAEVANTMNLTRLLNSGCLLLHYAGHGNNEYLAFESTTEWKCGIMEPLKVEYLKNLFLVGEIVKTQLVFMSSCSSEASGNVFVAAGVPHVVAVKHDAGVTDEVAREFATVFYDALFENGGRSTVKQAFDMARNCVNATHLGAPKHEEDLFLLLPRGKXXXXXXXXXAR--------ADGNHDVRIFETLPEGKLVDETQRLPPRPPCKKAVSNYMGQARVQHVVRLLLDEKAACVTVTGEPGSGKTELAVQACDYVRDRHHFNSFLWADCDKAALDASPYTSVSSADQFFVFPNSPNGASGDSVSLEDLCRQIGVAIGMPKPGPSTAKKLHDFIF--PDTDSGQPFQKVLLVLDNVDSLLERGGDAQDRLVHLLCDLCSMGGGGHLKLLATSEHKLLSGNEGFHGGTEMVTTVERLKTRHASELLMNNSPRDLQPEELGLRSGGLSKPHVVGAVQNHPVLKEVLEIAGGHPGTLVRLAPVLLDGPLDDARTLKEMATKCRADFRDKSYRQRGHRRSGSGTSAGSSSFAMIAREMSEASSSLGTSAPTSRSPNGSPAPATPSQRPAMRNAFSVDAGHRRDRGPVSLNHTNSSIDGSGXXXXXXXH---ARPLPPMPH---EEDGGEGLPRAYSSSPTGRCGNPRCHGRPQHQHSLRQESGSPPACCLPHQFAQELRDRVAMMYRRRMMAREALEEQPELPQMNMEQQHAWDMAEAAGVERGCRLVWVAATSNMANDRRXXXXXXXXXGWWAS---KQGEFVSWNCLREGLQRHLHVRMTVPCSSSGGDRHDALEGDHYTYLDDEQEVDSEEVXXXXXXXXXXXXQGRAAGGERRTGNRRMRPLPHATRVLDKPQLDFMRGILRKKQKELANAGETTVDCTEYGLDARIDFISVEAFAKYSRWWAPLMGTLAIIRNDWARTRPVRVHGFIGRLATDEKLLRRARGAFLLRFSERHPGKLVISRA-YHHSLVEVTEGGRCYMTVDQQGTRREYGSLHDLVTQNSQLKQLYPNIPKEQVFRGDAPATRPETETEGAREARRENGYELGAAS 1095
            L T +ELE +  TLRGCGRQ+ FRAEVANTMNLTRL+NSGCL+LHYAGHGN EYLAFESTTEW CGIMEPLKVEYL+NLF VG IV+TQLVF+ SCSSE SGNVFVAAGVPHVVAVKH++GVTD  AREFATVFYDAL ++    TVK+AFD A  C+NA H   PKHEEDLFLLLP+G+          R        A    D         GKLVDET+++PPRPPCKKAVS+YMG  RVQ VVRLLLD +  CVTVTGEPGSGKTELA+QACDYVR+RHHF+SFLWADCDKAALDA P T       FFV PNSPNGASGDSVSLEDLCR IGVAIGMP+PGPSTA +LH FIF  P+TDSGQPFQKVLLVLDNVDSL ER  DAQDRLVHLL DLCSMGGGGHLKLLATSE+KLL GN  F GG+EMV TV+ LK +HASELLM+NSPRDLQPEELGL+SGG SK  VV AV+NHPVLKEVL+IAGGHPGTLVRLAPVLL G L+DAR LKE+ATKCRA FRDKSYRQRGHRRSGS TSAGSS+   +ARE+SEA SSLGTSAPTSRSP+GSPAPATPSQRPAM NAFS DAGHRRDR  VSLN TNSSIDGSGXXXXXXX     RP         EEDGGEG PR +S       GN   HG  +    L+         C+ HQ + E +D V  M+RR+M+AREALEEQPELPQMN E QHAWDMAEAA VERG RLVW  ATS M +DRR         GW      K GE V    L E L+ HL VRMTVPCSSS G RHD LEG HYTYLDD +EVD EEV    XXXXXXX Q RAAG  RRTGNRRMRP+ HATRVLD+ QL F+R ILRK QK LA A   T D T   LD   D ISV  F+++S+WW PLM TL  IRNDWAR  P+RVHGFIGR    + LLR    AFLLRFSERHPGKLV+SRA +HHSLVEVTEGG+C+M VD++GTRR+Y SLHDLVTQNS L+QLYP+IPKEQVF+G APA  PETETEG  EA R NGY   A S
Sbjct:   11 LPTHQELEHLLETLRGCGRQVSFRAEVANTMNLTRLVNSGCLMLHYAGHGNGEYLAFESTTEWMCGIMEPLKVEYLENLFRVGGIVQTQLVFIGSCSSEVSGNVFVAAGVPHVVAVKHNSGVTDGAAREFATVFYDALIKH--EVTVKRAFDTALICINA-HRCPPKHEEDLFLLLPKGEEKSFGCCTAERLRLGRFQHAPFQEDQPFVVEHVGGKLVDETRQVPPRPPCKKAVSSYMGLVRVQFVVRLLLDPQTVCVTVTGEPGSGKTELAIQACDYVRERHHFDSFLWADCDKAALDACPET-------FFVIPNSPNGASGDSVSLEDLCRLIGVAIGMPQPGPSTANELHQFIFKNPNTDSGQPFQKVLLVLDNVDSLFERDEDAQDRLVHLLSDLCSMGGGGHLKLLATSEYKLLRGNGYFRGGSEMVATVKPLKMQHASELLMDNSPRDLQPEELGLQSGGFSKADVVSAVENHPVLKEVLKIAGGHPGTLVRLAPVLLHGSLNDARKLKEVATKCRASFRDKSYRQRGHRRSGSCTSAGSSN---VARELSEAGSSLGTSAPTSRSPHGSPAPATPSQRPAMGNAFSEDAGHRRDRRCVSLNRTNSSIDGSGXXXXXXXXXWGTRPXXXXXXXXXEEDGGEGRPRPHS-------GNVGSHGSLERHDWLQS--------CV-HQLSLERQDEVNGMHRRQMLAREALEEQPELPQMNTEMQHAWDMAEAARVERGSRLVWANATSYMVDDRRRNKGCGGG-GWGVGGRLKLGEAVPLKYLLESLRWHLDVRMTVPCSSSSGGRHDPLEGTHYTYLDDGEEVDFEEVEADDXXXXXXXDQERAAGEGRRTGNRRMRPMRHATRVLDERQLGFIRSILRKNQK-LAKAEANTQDSTACELDGLEDPISVGTFSRFSQWWTPLMVTLGNIRNDWARIWPIRVHGFIGRHEA-QALLRMKPTAFLLRFSERHPGKLVVSRACHHHSLVEVTEGGQCFMEVDKRGTRRKYESLHDLVTQNSLLRQLYPDIPKEQVFKGKAPAA-PETETEGTPEAPRGNGYGWCATS 1047          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16191.1 vs. uniprot
Match: D7FNY4_ECTSI (SH2 domain containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FNY4_ECTSI)

HSP 1 Score: 807 bits (2085), Expect = 3.550e-275
Identity = 530/1074 (49.35%), Postives = 626/1074 (58.29%), Query Frame = 0

Query:   59 LRGCGRQIKFRAEVANTMNLTRLLNSGCLLLHYAGHGNNEYLAFESTTEWKCGIMEPLKVEYLKNLFLVGEIVKTQLVFMSSCSSEASGNVFVAAGVPHVVAVKHDAGVTDEVAREFATVFYDALFENGGRSTVKQAFDMARNCVNATHLGAPKHEEDLFLLLPRGKXXXXXXXXXARADGNHDVRIFETLPEGKLVDETQRLPPRPPCKKAVSNYMGQARVQHVVRLLLDEKAACVTVTGEPGSGKTELAVQACDYVRDRHHFNSFLWADCDKAALDASPYTSVSSADQFFVFPNSPNGASGDSVSLEDLCRQIGVAIGMPKPGPSTAKKLHDFIF--PDTDSGQPFQKVLLVLDNVDSLLERGGDAQDRLVHLLCDLCSMGGGGHLKLLATSEHKLLSGNEGFHGGTEMVTTVERLKTRHASELLMNNSPRDLQPEELGLRSGGLSKPHVVGAVQNHPVLKEVLEIAGGHPGTLVRLAPVLLDGPLDDARTLKEMATK----------------------------------------CRADFRDKSYRQRGHRRSGSGTSAGSSSFAMIAREMSEASSSLGTSAPTSRSPNGSPAPATPSQRPAMRNAFSVDAGHRRDRGPVSLNHTNSSIDGSGXXXXXXXHARPLPPMPHEEDGGEGLPRAYSSSPTGRCGNPRCHGRPQHQHSLRQESGSPPACCLPHQFAQELRDRVAMMYRRRMMAREALEEQPELPQMNMEQQHAWDMAEAAGV-ERGCRLVWVAATSNMANDRRXXXXXXXXXGWWASKQGEFVSWNCLREGLQRHLHVRMTVPCSSSGGDRHDALEGDHYTYLDDEQEVDSEEVXXXXXXXXXXXXQGRAAGGERRTGNRRMRPLPHATRVLDKPQLDFMRGILRKKQKELANA--GETTVDCTEYGLDARIDFISVEAFAKYSRWWAPLMGTLAIIRNDWARTRPVRVHGFIGRLATDEKLLRRARGAFLLRFSERHPGKLVIS------------RAYHHSLVEVTEGGRCYMTVDQQGTRREYGSLHDLVTQNSQLKQLYPNIPKEQVFRGDAPATRPET 1075
            LR  GRQI+FRAEVAN +NL+RLL SGC +LH+AG GN++YLAFES  +W CGIMEPL+V  L NLF  G  V+T+LVF+SSCSSE SG   V AGVPHVVAVKH A VT                                                                      GNHDVRIFETLPEG+LVDET    PRPPCKKAV +Y G A +Q VVRLLLDE+AACVT+TGEPGSGKTE A+QACDYVR+RH F+ FLWADCDKAAL++    S  S + FF  P     ++      ED CR IG+AIGMP+PGP++ ++LH FIF  P+TDSGQPFQKVLLVLDNVDSLLER GDAQDRLV LLC+LCS+G GGHLKLLATSEHKLLSG+E F GGTE V  VE L+TRHASELLM+N PR+L+  ELGL SGGLS   V+ AVQNHPVLKEVLEIA GHPGTLVRLAPVLLDG LDDA  LKEM T+                                        CRADFRDKSYR+R + R  S  +A SS          E S ++  S+ T        AP +P     M  AF  D   +R+RG       +SS     XXXX      P+ P+P+E    EG  RA+SSSP  RC NP  HG PQ           PP   L HQ  Q+          RR++A+EA +++PE PQM+ME+Q   D+A AAGV +RGCRLVWV ATS MA   R   XXXXXX W +S++G+ VSW CL+E L  HL                   EG   T   D+  +                                    PH  RV D PQL+F+RGILR+ Q+ +A A  G T  +C E     R DFISVEAFAK+S+WWAPL+ TL IIRNDWARTRPVRVHGF+ RLA    L++R  G FLLRFSE   G LVIS             +  HSLVEV  GGRC +  ++ G R  Y SLHD V +  +LK+LYP+IPKE+ F G+ P + P T
Sbjct:    2 LRESGRQIRFRAEVANIINLSRLLKSGCRMLHFAGQGNDKYLAFESGRDWNCGIMEPLEVASLSNLFQAGG-VRTELVFISSCSSEMSGRACVEAGVPHVVAVKHKANVTG---------------------------------------------------------------------GNHDVRIFETLPEGQLVDETHPPTPRPPCKKAVPSYKGLAGLQQVVRLLLDERAACVTITGEPGSGKTERAIQACDYVRERHRFDCFLWADCDKAALNSC---SDKSGNPFFALPTCLKDSA-----WEDPCRLIGLAIGMPQPGPTSEQELHQFIFNNPNTDSGQPFQKVLLVLDNVDSLLERDGDAQDRLVQLLCNLCSIGDGGHLKLLATSEHKLLSGHERFRGGTETVARVEPLETRHASELLMDNLPRNLELTELGLESGGLSLADVLTAVQNHPVLKEVLEIAEGHPGTLVRLAPVLLDGSLDDACRLKEMTTRRVVVAARVMVWRLFEWLLLLGATKVAYNWDLATIGSLSEECRADFRDKSYRRRRYGRPNSNRTAVSSKG--------ELSVTMTLSSSTG-------APRSPMILSPMGPAFG-DTHDQRNRGDTRFLQRDSSSXXXXXXXXRAGMTSPVRPVPNETVR-EGPARAHSSSPPARCRNPEWHGPPQ-----------PPPHSLLHQQLQD---------HRRVLAQEAPDDEPESPQMDMEEQRTRDLARAAGVVDRGCRLVWVTATSRMAGGCRQDDXXXXXXXW-SSRRGKLVSWECLQESLMEHLR------------------EGTKTTRNWDDPIIS-----------------------------------PHG-RVADTPQLEFIRGILRRGQEAVARARWGATGDECPEVDGGCRYDFISVEAFAKFSQWWAPLISTLGIIRNDWARTRPVRVHGFMSRLAAKRLLMQRECGTFLLRFSESKMGALVISFTQPSVSPGRRSLSVKHSLVEVKWGGRCCIQAEKGGMRC-YASLHDFVRRIPELKKLYPDIPKEEAF-GETPRSPPAT 903          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16191.1 vs. uniprot
Match: D7FNX8_ECTSI (SH2 domain containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FNX8_ECTSI)

HSP 1 Score: 499 bits (1285), Expect = 5.840e-156
Identity = 396/1112 (35.61%), Postives = 532/1112 (47.84%), Query Frame = 0

Query:   55 VRNTLRGCGRQIKFRAEVANTMNLTRLLNSGCLLLHYAGHGNNEYLAFESTTEWKCGIMEPLKVEYLKNLFLVGEIVKTQLVFMSSCSSEASGNVFVAAGVPHVVAVKHDAGVTDEVAREFATVFYDALFENGGRSTVKQAFDMARNCVNATHLGAPKHEEDLFLLLPRGKXXXXXXXXXARADGNHDVRIFETLPEGKLVDETQRLPPRPPCKKAVSNYMGQARVQHVVRLLLDEKAACVTVTGEPGSGKTELAVQACDYVRDRHHFNSFLWADCDKAALDASPYTSVSSADQFFVFPNSPNGASGDSVSLEDLCRQIGVAIGMPKPGPSTAKKLHDFIF--PDTDSGQPFQKVLLVLDNVDSLLERGGDAQDRLVHLLCDLCSMGGGGHLKLLATSEHKLLSG-NEGFHGGTEMVTTVERLKTRHASELLMNNSPRDLQPEELGLR-SGGLSKPHVVGAVQNHPVLKEVLEIAGGHPGTLVRLAPVLLDGPLDDARTLKEMATKCRADFRDKSYRQRGHRRSGSGTSAGSSSFAM--------IAREMSEASSSLGTSAPTSRSPNGSPAPATPSQRPAMRNAFSVDAGHRRDR---------------------GPVSLNHTNSSIDGSGXXXXXXXHARPLPPMPHEEDGGEGLPRAYSSSPTGRCGNPRCHGRPQHQHSLRQESGSPPACCLPHQFAQELRDRVAMMYRRRMMAREALEEQPELPQMNMEQQHAWDMAEAAGV-ERGCRLVWVAATSNMANDR----RXXXXXXXXXGWWASKQG-------EFVSWNCLREGLQRHLHVRMTVPCSSSGGDRHDALEGDHYTYLDDEQEVDSEEVXXXXXXXXXXXXQGRAAGGERRTGNRRMRPLPHATRVLDKPQLDFMRGILRKKQKEL-----------ANAGETTVDCTEYGLDARID------------------FISVEAFAKYSRWWAPLMGTLAIIRNDWARTRPVRVHGFIGRLATDEKLLRRARGAFLLRFSERHPGKLVISRAYH----------------------------HSLVEVTEGGRCYMTVDQQGTRREYGSLHDLVTQNSQLKQLYPNIPKEQVF 1064
            +R  LR  GRQI+FR+EVA   N   LLNSGC +LHY GHG+NE+LAFES  +  CGIMEPL V  LKNLF  G  V+T+LVF+SSCSSEASGN FV AGVPHVVAVK +  VTD+ A++FA  FYDAL    GR TVKQAFD+A N VNA H    + + D FLLLP G              GNHDVRIF+TLPEG+ VDET+  P RP C + V  Y G                                                                                         LE+L  QIG+AIGM +PGP++ ++L  F+F  P    G+  QKVLLVL++VDSL E GGDA+DR++ LL +LCS G   HLKLL TSE +L    NE F  G+E V  V+ LK R A++LL+N  P     +EL L  S   ++  ++ A++ HPVLK     A GHPGTL+RLAP+L D  ++DA  L   A + R +++ +++  R   R+GS TS  SS+ A         +A     +  SLG S    R       P TP+Q   + +A S D   ++ +                     G   ++H    ++G+G       H+  +        GG G     +++P     +   H      H  R+ S  P +   P Q   + R    M    ++MA+  LEE P+ PQM  E++ AWD A  AG+ +RGCRLVWV AT+N   +               GW A   G       + V W+ LR GL +HL  ++T+P S       + ++   Y +  D   V                  G   G      N          R +++ +L F+RG+L+ KQ+++           A+ G    +    G     D                   IS++AF ++S WWAPLM TL+++RNDWA T P RVHGFI R+A + +LL R RG FLLRFSE  PG+LV+S   H                            H LVEV  GG CY+ V+Q G++  Y SLHDLV   S ++ LYP++ KE  F
Sbjct:    2 IRMKLRESGRQIRFRSEVAKPSNFRELLNSGCRMLHYTGHGSNEFLAFESNDDRMCGIMEPLHVGPLKNLFQAGG-VRTELVFISSCSSEASGNAFVDAGVPHVVAVKREESVTDQAAQQFAKQFYDALLSEAGRFTVKQAFDIALNTVNA-HPPHARPQGDHFLLLPHG--------------GNHDVRIFDTLPEGRFVDETREPPARPRCARPVPFYQG-----------------------------------------------------------------------------------------LEEL--QIGLAIGMTQPGPNSEEELVRFLFDRPTVQEGRTCQKVLLVLEDVDSLFEAGGDARDRVLKLLSNLCSKGE--HLKLLVTSEQRLQRDTNERFRHGSERVAKVKPLKDRDAAQLLINKVPTRFTKKELRLSLSHRCTREDILKALEAHPVLKA----AEGHPGTLLRLAPLLEDHNVNDACVLHR-ANQHRLEYKQETHTLR-RNRAGSNTSLASSAGAAGTGMGGGGVALPSGASDMSLGNSMGGGR-------PMTPTQPHLLGHAMSHDTHFQQQQXXXXXXXXXVSPFFQDLSSWAGGGEMHHP--LLNGAGMAGGSSIHSSGI--------GGSGHGIHSAAAPAAARASSHPH------HHQRRHSSHPQSPTHPRQHHPQRR----MTVMNQLMAQVELEE-PDPPQMTPEEKRAWDTARTAGLMDRGCRLVWVKATTNALLNGWTYDGGGGSDNGGFGWPAEGAGAGGVTNYDCVPWDYLRRGLMQHLCGKLTIPSSHD-----EVVDEGPYGWRGDASSV------------------GVGVGNGYGAVN------TPQVRWMNEEELAFVRGVLKAKQEKVRLKQEKLRQQRASRGYAGTELVSIGSSGAADAASGRXXXXXXXXXXXXDVISLDAFVEFSLWWAPLMPTLSLLRNDWASTNPTRVHGFISRIAAERQLLERERGTFLLRFSESQPGELVVSFTEHVTQAAPRASSMLTSAPRSRPRSQSMCVKHCLVEVRRGGWCYIEVEQ-GSKVPYPSLHDLVLACSSMQVLYPDVRKEDAF 940          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16191.1 vs. uniprot
Match: A0A6H5KH01_9PHAE (CHAT domain-containing protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KH01_9PHAE)

HSP 1 Score: 482 bits (1240), Expect = 3.800e-152
Identity = 341/794 (42.95%), Postives = 440/794 (55.42%), Query Frame = 0

Query:   22 LGFIHARPLVRFNDNGTKFWELEPLRTCEELEFVRNTLRGCGRQIKFRAEVANTMNLTRLLNSGCLLLHYAGHGNNEYLAFESTTEWKCGIMEPLKVEYLKNLFLVGEIVKTQLVFMSSCSSEASGNVFVAAGVPHVVAVKHDAGVTDEVAREFATVFYDALFENGGRSTVKQAFDMARNCVNATHLGAPKHEEDLFLLLPRGKXXXXXXXXXARADGNHDVRIFETLPEGKLVDETQRLPPRPPCKKAVSNYMGQARVQHVVRLLLDEKAACVTVTGEPGSGKTELAVQACDYVRDRHHFNSFLWADCDKAALDASPYTSVSSADQFFVFPNSPNGASGDSVSLEDLCRQIGVAIGMPKPGPSTAKKLHDFIF--PDTDSGQPFQKVLLVLDNVDSLLERGGDAQDRLVHLLCDLCSMGGGGHLKLLATSEHKLLSG-NEGFHGGTEMVTTVERLKTRHASELLMNNSPRDLQPEELGLR-SGGLSKPHVVGAVQNHPVLKEVLEIAGGHPGTLVRLAPVLLDGPLDDARTLKEMATKCRADFRDKSYRQRGHRRSGS---GTSAGSSSFAM----IAREMSEASSSLGTSAPTSRSPNGSPAPATPSQRPAMRNAFSVDA-------GHRRDRGPVSLNHTNSSIDGSGXXXXXXXHARPLPPMPHEEDGGEGLPRAYSSSPTGRCGNPRCHG-----------RPQHQHSL--RQESGSPPACCLPHQFAQELRDRVAMMYRRRMMAREALEEQPELPQMNMEQQHAWDMAEAAGV-ERGCRLVWVAATSN 783
            LG++ + PLV + +NG +   +E L T EEL+ +R  LR  GRQI+FR+EVA   N   LLNSGC +LHY GHGNNE+LAFES  +  CGIMEPL V  LKNLF  G  V+T+LVF+SSCSSEASGN FV AGVPHVVAVK +  VTD+ A++FA  FYDAL    G  TV+QAFD+A N VNA H    + + D FLLLP G              GNHDVRIF+TLPEG  VDET+  P RP C + V  Y G   +Q VV +L+D   ACVTVTGE GSGK+E A+QACDYVR RHHF++  WAD   A       T+++S+  F+                +D CR IG+AIGM +PGPS+ ++L  F+F  P    G+   KVLLVLD+VDSL E GGDA+DR++ LL +LCSM    HLKLL TSE  L    NE F  G+E V  V  L  R A++LL++  P     +EL L  S   ++  ++ A++ H VLK     A GHPGTL+RLAP+L D  ++DA  L E  T+CR +++ +    R +R S +    +S G++   M    +A     +  SLG S    R       P TP+Q   + +A S D        G     G        SS  G G              M H    G G+    S   +G  G+   HG           RP        R+ S    +   PHQ   + R  V      ++MA+  LEE P+ PQM   ++ AWD A  AG+ +RGCRLVWV AT+N
Sbjct:   27 LGYVFSSPLVSWGENG-RMKRMEQLGTSEELKMIRMKLRESGRQIRFRSEVAKPSNFRELLNSGCRMLHYTGHGNNEFLAFESNDDRMCGIMEPLHVGPLKNLFQAGG-VQTELVFISSCSSEASGNAFVDAGVPHVVAVKREESVTDQAAQQFAKQFYDALLSEAGSFTVQQAFDIALNTVNA-HPPYARPQGDHFLLLPHG--------------GNHDVRIFDTLPEGSFVDETREPPARPRCARPVPFYQGLEELQKVVEMLVDPVTACVTVTGEHGSGKSERAIQACDYVRMRHHFDALWWADMKHAMA-----TAINSSVWFY----------------DDPCRLIGIAIGMTQPGPSSEEELVRFLFDRPTVQEGRTCHKVLLVLDDVDSLFEAGGDARDRVLKLLSNLCSMSE--HLKLLVTSEQSLQRDTNERFRHGSERVAKVRPLNDRDAAQLLLDKVPTKFTKKELRLSLSHRCTREDILKALEAHRVLKA----AEGHPGTLLRLAPLLEDHNVNDACVL-ERVTQCRLEYKLEPQTLRRNRASSNTSLASSVGAAGTGMGGGGVALPSGASDMSLGNSMGGGR-------PMTPTQPHFLGHAISHDTHFQQQHNGTGNGNGASPFFQEFSSWAGGGE-------------MHHPLLNGAGMAGGSSIHSSGIGGSG--HGTHSAAXXXXXXRPSXXXXXXXRRHSAHLQSPTHPHQHRPQRRMTVV----NQLMAQVELEE-PDPPQMTPREKRAWDTARTAGLMDRGCRLVWVTATTN 748          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16191.1 vs. uniprot
Match: A0A6H5JPT3_9PHAE (SH2 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JPT3_9PHAE)

HSP 1 Score: 258 bits (658), Expect = 4.990e-72
Identity = 191/498 (38.35%), Postives = 239/498 (47.99%), Query Frame = 0

Query:  635 RRDRGPVSLNHTNSSIDGSGXXXXXXXHARPLPPMPHEEDGGEGLPRAYSSSPTGRCGNPRCHGRPQHQHSLRQESGSPPACCLPHQFAQELRDRVAMMYRRRMMAREALEEQPELPQMNMEQQHAWDMAEAAGV-ERGCRLVWVAATSNMANDRRXXXXXXXXXGWWASKQGEFVSWNCLREGLQRHLHVRMTVPCSSSGGDRHDALEGDHYTYLDDEQEVDSEEVXXXXXXXXXXXXQGRAAGGERRTGNRRMRPLPHATRVLDKPQLDFMRGILRKKQKELANA--GETTVDCTEYGLDARIDFISVEAFAKYSRWWAPLMGTLAIIRNDWARTRPVRVHGFIGRLATDEKLLRRARGAFLLRFSERHPGKLVIS------------------------------------------------------RAYHHSLVEVTEGGRCYMTVDQQGTRREYGSLHDLVTQNSQLKQLYPNIPKEQVFRGDAPATRPET 1075
            +R+RG       +SS  G             + P+P E    EG  RA+SSSP  RC NP  HG+PQ           PP   L HQ  QE R         R+ A+EA   +PE  QM++++Q AWD+A AAGV +RGCRL+WV ATS MA   R         GW +S++GE VSW CL+E L  HL                   EG   T   D+  +                                   LPH  RV+D PQL+F+RGILR+ Q+ +A A  G T  +CTE G D R DF+SVEAFA +S+WWAPLM TL IIRNDWA TRPVRVHGF+ R+A+   L++R  G FLLRFSE   G LVIS                                                       +  HSLVEV  GGRC +  ++ G R  YGSLHD V Q  +LK+LYP+IPKE+ F G+ P + P T
Sbjct:   29 QRNRGDTRFLQRDSSSGGGSGGSGRAGMTSLVRPVPSETVR-EGPARAHSSSPPARCRNPEWHGQPQ-----------PPPHSLLHQQLQEYR---------RVSAQEAPCIEPESSQMDLKEQRAWDLARAAGVVDRGCRLIWVTATSRMARGCRQDDGGGSGGGW-SSRRGEIVSWECLQESLMEHLR------------------EGTKTTRNWDDPII-----------------------------------LPHG-RVVDTPQLEFIRGILRRGQEAVAKARWGATGDECTEVGGDGRDDFVSVEAFANFSQWWAPLMATLGIIRNDWATTRPVRVHGFMSRVASKRLLMQRECGTFLLRFSESRMGALVISFTQPSVSVSRLSRISRCLVPFISCAGRLACCLQGSPINPRIVLQQFQLRSGRRSLSVKHSLVEVKWGGRCCIQAEKGGMRC-YGSLHDFVRQIPELKKLYPDIPKEEAF-GETPRSPPAT 448          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16191.1 vs. uniprot
Match: A0A6H5JKN2_9PHAE (CHAT domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKN2_9PHAE)

HSP 1 Score: 245 bits (626), Expect = 4.700e-71
Identity = 130/188 (69.15%), Postives = 149/188 (79.26%), Query Frame = 0

Query:   40 FWELEP-LRTCEELEFVRNTLRGCGRQIKFRAEVANTMNLTRLLNSGCLLLHYAGHGNNEYLAFESTTEWKCGIMEPLKVEYLKNLFLVGEIVKTQLVFMSSCSSEASGNVFVAAGVPHVVAVKHDAGVTDEVAREFATVFYDALFENGGRSTVKQAFDMARNCVNATHLGAP-KHEEDLFLLLPRGK 225
            F+EL   L T +ELE +RNTLR CGRQI+FRAEVANTMNLTRLL SGC +LH+AG GN++YLAFES  +W CGIMEPL+V  LKNLF  G  V+T+LVF+SSCSSE SG   V AGVPHVVAVKH+A VTDE A  F+TVFYDAL ENGGR TVKQAFD+A N VNATH  AP +H    F+LLP+GK
Sbjct:   20 FFELATNLPTHQELELIRNTLRECGRQIRFRAEVANTMNLTRLLCSGCRMLHFAGQGNDKYLAFESGRDWNCGIMEPLEVASLKNLFQAGG-VRTELVFISSCSSEMSGRACVEAGVPHVVAVKHEANVTDEEALHFSTVFYDALLENGGRYTVKQAFDIALNFVNATHSSAPNRHAGGHFVLLPKGK 206          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16191.1 vs. uniprot
Match: A0A6H5JKD1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKD1_9PHAE)

HSP 1 Score: 236 bits (601), Expect = 6.050e-68
Identity = 123/161 (76.40%), Postives = 137/161 (85.09%), Query Frame = 0

Query:  379 MPKPGPSTAKKLHDFIF--PDTDSGQPFQKVLLVLDNVDSLLERGGDAQDRLVHLLCDLCSMGGGGHLKLLATSEHKLLSGNEGFHGGTEMVTTVERLKTRHASELLMNNSPRDLQPEELGLRSGGLSKPHVVGAVQNHPVLKEVLEIAGGHPGTLVRLAP 537
            M +PGP++ ++LH FIF  P+TDSGQPFQKVLLVLD+VDSLLER GDAQDRLV  LC+LCSMG GGHLKLLATSEHKLLSGNE F GGTEMV  VE L+TRHASELLM+N PR+L+  ELGL+SGGLS   V+ AVQNHPVLKEVLEIA GHPGTLVR  P
Sbjct:    1 MSQPGPNSEQELHQFIFKNPNTDSGQPFQKVLLVLDSVDSLLERDGDAQDRLVQFLCNLCSMGDGGHLKLLATSEHKLLSGNEFFRGGTEMVARVEPLETRHASELLMDNLPRNLKLRELGLQSGGLSLADVLSAVQNHPVLKEVLEIAEGHPGTLVRDEP 161          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16191.1 vs. uniprot
Match: A0A6H5JN51_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JN51_9PHAE)

HSP 1 Score: 170 bits (431), Expect = 3.110e-45
Identity = 86/137 (62.77%), Postives = 101/137 (73.72%), Query Frame = 0

Query:  237 ADGNHDVRIFETLPEGKLVDETQRLPPRPPCKKAVSNYMGQARVQHVVRLLLDEKAACVTVTGEPGSGKTELAVQACDYVRDRHHFNSFLWADCDKAALDASPYTSVSSADQFFVFPNSPNGASGDSVSLEDLCRQI 373
            A GNHD+RIFETLPEG+LVDET    PRPPCKKAV ++ G A VQ VVRLLL+ +AACVTVTGE GSGKTE A+QACDYVR+RHHF+SFLWADCDKAA ++   +  SS   FF   N  N ++      ED CR +
Sbjct:   29 AGGNHDIRIFETLPEGQLVDETHPPTPRPPCKKAVPSFKGLAGVQQVVRLLLNPQAACVTVTGEHGSGKTERAIQACDYVRERHHFDSFLWADCDKAAFNSC--SDKSSGHPFFALQNCLNDSA-----WEDPCRLV 158          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16191.1 vs. uniprot
Match: A0A7S0DT24_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Amorphochlora amoebiformis TaxID=1561963 RepID=A0A7S0DT24_9EUKA)

HSP 1 Score: 164 bits (416), Expect = 1.050e-39
Identity = 151/490 (30.82%), Postives = 223/490 (45.51%), Query Frame = 0

Query:   24 FIHARPLVRFNDNGTKFWELEPLRTCEELEFVRNTLRGCGRQIKFRAEVANTMNLTRLLNSGCLLLHYAGHGNNEYLAFESTTEWKCGIMEPLKVEYLKNLFLVGEI-VKTQLVFMSSCSSEASGNVFVAAGVPHVVAVKHDAGVTDEVAREFATVFYDALFENGGRSTVKQAFDMARNCV-NATHLGAPKHEEDLFLLLPRGKXXXXXXXXXARADGNHDVRIFETLPEGKLVDETQRLPPRPPCKKAVS-NYMGQARVQH-VVRLLLDEKAACVTVTGEPGSGKTELAVQACDYVRDRHHF-NSFLWADCDKAALDASPYTSVSSADQFFVFPNSPNGASGDSVSLE-------------DLCRQIGVAIGMPKPGPSTAKKLHDFIFPDTDSGQPFQKVLLVLDNVDSLLERGG----DAQDRLVHLLCDLCSMGGGGHLKLLATSEHKLLSGNEGFHGGTEMVTTVERLKTRHASELLMNNSPRDL 491
            ++ + PLV  N       E++ L    E E ++ TL    ++++ R+  A        L  GC +LHYAGHG    LAFE+      G    +    L  +F VG    + +LVF+++C SE S   FV AGV HVV VK DA + D+ A  F T FY AL  N    TVK+AF +A+N V N T  G  K E + +LLLP              A+GNHDVRI+E +P G + D T   PP      AVS N +G+    H VV  ++D+K+  +T+TGE G G+ E+AV A  Y   R  F +  ++ D     L  SP T V    +     ++P  + G   S +                +++  A  +     S A KL              ++VLLV   +   +++      D  + L+  L   C +G    +KLL TS   + S         E V ++  L+   A  L +N++PR +
Sbjct:   18 YMASSPLVDHNGR-----EIDRLDVSGEWESLKKTLANANKKLRIRSIYATPEIFRETLTLGCRVLHYAGHGLPSALAFENGE----GATHLINRSTLNQIFAVGNSHERVRLVFVAACHSEVSAEAFVGAGVKHVVGVKRDAKLNDKAALTFTTQFYLALMVN----TVKEAFQIAKNSVANLTDYG--KGESEKYLLLP--------------ANGNHDVRIYEDIPRGTMSDHTS--PPSVNNLPAVSPNLLGRGEATHAVVSRIVDQKSRVITLTGEAGIGRREVAVAASQYAVQRQIFADGVVYID-----LSRSPRTLVGQIAEVLGINSNPPKSEGKLKSTQASYINSDIKFRTTQTLQELSQAQPLTLTLTSLATKLQT------------KRVLLVAPGLSDAVKKSNPDWRDTFNALIEKLLGSC-LG----VKLLVTSRVPIKSLLLPKKSLLESVVSIFPLERSQALSLFLNSAPRPI 454