mRNA_E-siliculosus-1a_M_contig8.16098.1 (mRNA) Ectocarpus siliculosus Ec864m_EcPH12_78m male

BLAST of mRNA_E-siliculosus-1a_M_contig8.16098.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 1717 bits (4447), Expect = 0.000e+0
Identity = 895/929 (96.34%), Postives = 906/929 (97.52%), Query Frame = 1

Query:    1 AIRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSWEDFTTFGAFPSALKIAPGLLDHGSCDAPAAARDATTTTSDCSAGRLSTTHGASMQADSAHGLMVELSPGTLPSHGGGDGDEAKGDEFIQELLKSLSSPSLDLHAMTCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAGNDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLGHGDGTEVEHGYYFDFVASSNPSFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSGMPFVSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCADTSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGDQCLPDDTADTLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTSLDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDSSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSNGKLKFNFNVDPDAGLELRATLSWNDPAASSLSGDQLINDLDLRVYAPDGTKYCMWDDNCWDTSNVNERVIVPADVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISPPIQGSSAXXXXXXXXXXXXXXXXATGAAVTMYGRGAGAGSGFVLAGVVMLAAALAAAV 2787
            A RKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSWEDFTTFGAFP+ALKIAPGLLDHGSCD PAAARDA TTTSDCSAGRLSTTHGASMQ DSAHGLMVELSPGTLPSH GGD   AK  EFIQELLKSLSSPSLDLHA+TCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHA NDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSF ASRHEVLKV GWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFL HGDGTEVEHGYYFDFVASSNP FTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSGMPFVSADSFVSDDDLGHGTH ATTVAGSTISSPAELVSCAD SEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGDQCLPDDTADTLSEHGGMAQGAKIAAID+FYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLT+LDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDSSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFY ADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYS+ KLKF FNVDPDAG+ELRATLSWNDPAASSLSGDQL+NDLDLRVYAPDGT+YCMWDDNCWDTSNVNERVIVPA+VVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISPPI+G+SA  XXXXXXXXXXXXXXATGAAVTMYGRGAGAGSGFVLAGV MLAAALAAAV
Sbjct:   78 AYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSWEDFTTFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGD---AKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSGMPFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGDQCLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDSSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISPPIEGNSAVVXXXXXXXXXXXXXXATGAAVTMYGRGAGAGSGFVLAGVAMLAAALAAAV 1003          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16098.1 vs. uniprot
Match: A0A6H5L2G3_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L2G3_9PHAE)

HSP 1 Score: 1278 bits (3306), Expect = 0.000e+0
Identity = 670/694 (96.54%), Postives = 680/694 (97.98%), Query Frame = 1

Query:  712 MACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLGHGDGTEVEHGYYFDFVASSNPSFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSGMPFVSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCADTSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGDQCLPDDTADTLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTSLDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDSSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSNGKLKFNFNVDPDAGLELRATLSWNDPAASSLSGDQLINDLDLRVYAPDGTKYCMWDDNCWDTSNVNERVIVPADVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISPPIQGSSAXXXXXXXXXXXXXXXXAT--GAAVTMYGRGAGAGSGFVLAGVVMLAAALAAAV 2787
            MACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFL HGDGTEVEHGYYFDFVASSNPSF SSSESRSMFKAEFTGGDF YDSDRRKVIQYIDLINPDTGDGSSSGMPFVSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCAD  EVIGCAGGCI ESSTGS+EADYGEDINRLCPAFGCSGDQCLPDDTA+TLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLT+LDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGG+SSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPD+ERGFGRIHLEAGMPLGGAGDM LFVADSSDHEV SNGKLKFNFNVDPDAGLELRATLSW+DPAASSLSGDQL+NDLDLRVYAPDGT+YCMWDDNCWDTSNVNERVIVPA+VVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISPPI+GSSAXXXXXXXXXXXXXXXX    GAA TMYGRGAGAGSGFVLAGV MLAAALAAAV
Sbjct:    1 MACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPSFDSSSESRSMFKAEFTGGDFFYDSDRRKVIQYIDLINPDTGDGSSSGMPFVSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCADNGEVIGCAGGCIIESSTGSTEADYGEDINRLCPAFGCSGDQCLPDDTANTLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGNSSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDSERGFGRIHLEAGMPLGGAGDMVLFVADSSDHEVSSNGKLKFNFNVDPDAGLELRATLSWSDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISPPIEGSSAXXXXXXXXXXXXXXXXXXXXGAAATMYGRGAGAGSGFVLAGVAMLAAALAAAV 694          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16098.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 785 bits (2028), Expect = 2.270e-268
Identity = 449/889 (50.51%), Postives = 584/889 (65.69%), Query Frame = 1

Query:   10 KLEGFLGARAVKPVSSTATHGACFMVTASPAEA-AQLSSWEDF--TTFGAFPSALKIAPGLLDHGSC----DAPAAARDATTTTSDCSAGRLSTTHGASMQADSAHGLMVELSPGTLPSHGGGDGDEAKGDEFIQELLKSLSSPSLDLHAMTCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAGNDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLGHGDGTEVEHGYYFD--FVASSNPSFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTG----DGSSSGM-PFVSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCADTSEVIGCAGGCITESSTGSSEADYG-----EDINRLCPAFGCSG--DQCLPDDTADTLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTSLDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSNGKLKFNFNVDPDAGLELRATLSWNDPAASSLSGDQLINDLDLRVYAPDGTKYCMWDDNCWDTSNVNERVIVPADVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAIS 2610
            +L+ FL   AV+ VS +  HGACF+ TAS A+A A +   E F   +F  FPSALK+APGL+DH         P++ +D           RL   HG +M+  +  GL V+L+P TLP+   G       + FI +LL  L S S+DLH+   WSD VM GG+HL S  GA+R R+WS+AA++V+E SE+   +P  +C W++V +  AG+D LLV GLDHLL+ GR   G+ EEE+ EL +AC+MGLVSFFA R EVL+V+  + K  LN AAR  IQSAS   TPLTDAGLDG+GEVIQ++D G++ET+CF     G EV+HGY+F+  FV S+         S+ ++   F  GDF+Y  +RRKVIQYI+L+NPD+       +  G+ PF+  D F  DD  GHGTHTA + AG+T++ PAE ++C+   E +GC GGCI ES T      Y       D++RLCP +GC G  D CL D+  +TL+++GGMAQGAK++  D+F+  +   ++ GNG+WE C +AGCK+HS S G D  C     ++LYD++MYEN E+LL+F+AGN G  T+R  CT+ SP I KNVL VG+++SG TR+ +T+  G G         ID+V++FS YGFT DGRIKPEVVAPGDM+YSA+ +G DS SC L AY GTS S  + +GAAAMVRQYF D +FY ADV +RGFC  GF C+ FSP A TVKA+LINSAN+MG  ++PD  RGFGRIHLE GMPL G GD+ALFVAD+ D  +  +   ++ F+VD DAGLELRATLSW D  A+S S  QL+NDLDL V +P GT Y MW  +  D  NVNERVIV AD V     T  W V V      V D+ S+SLVV GAIS
Sbjct:   93 RLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQD-----------RLGARHGGTMRMSNVEGLNVKLTPDTLPARSAG------AESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTG-------SSQLIYYPVFEAGDFTYTEERRKVIQYIELVNPDSDYVAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSG-DETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTDPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDG-ETERSVCTMASPAIGKNVLAVGASTSGDTRVISTE-GGAG---------IDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVE----TGTWTVLVYSNSL-VTDSQSYSLVVNGAIS 940          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16098.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 746 bits (1925), Expect = 3.150e-253
Identity = 438/883 (49.60%), Postives = 557/883 (63.08%), Query Frame = 1

Query:   10 KLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQL-SSWEDF--TTFGAFPSALKIAPGLLDHGSCDAPAAARDATTTTSDCSAGRLSTTHGASMQADSAHGLMVELSPGTLPSHGGGDGDEAKGDEFIQELLKSLSSPSLDLHAMTCWSDLVMKG-GDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAGNDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLGHGDGTEVEHGYYFDFVASSNPSFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSGMPFVSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCADTSEVIGCAGGCI-----TESSTGSSEADYGEDINRLCPAFGCSGDQ---CLPDDTADTLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTSLDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTR-LSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSNGKLKFNFNVDPDAGLELRATLSWNDPAASSLSGDQLINDLDLRVYAPDGTKYCMWDDNCWDTSNVNERVIVPA-DVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISP 2613
            +L+ FL   AV+ VS +  HGACF  +AS A+AA +    E F    F  FPS LK+APGL+DH       A  +A ++  D    RLS  HGA M+  +  GL VEL+PGTL +       EAK   FI  +L  L S S+DLH+   WSD  M G G+HL S AGA+R R+W +AA++V+E SE+    P  +C W ++ VHHAG+D LLV+GLDHLL+ GR   G+ EEE+ EL +AC+MGLVSF A R EVL+V+    K  LNAAAR  IQSAS   TPLTDAGLDG+GE+IQ+ID+G++ET+C+     G EV+HGYYFD +     +  SSS S       F GGDFSY  +RRK                                  GHGTHTA + AG+T++ PAE ++C + ++ + C G CI     T+     S    G D++RLCP +GC       CL DD  +TLS +GGMAQGAK+A  D+FYG Y      GN LWE C +AGCK+HSNS G D  C + S D+L D++MYEN E+LL+F+AGN G   +R TCTI++PGI KN L +G+++SG TR +ST    G           ID V+EFS +GFT D RIKPEVVAPGD +YSA+ +G D+ SC L AY GTS S  + +GAAAM+RQYF D +FY ADV +RGFC  GF C+ FSP A TVKA+LINSAN+MGG +EPD  RGFGR+HLEAGMPL G GD+ LFVAD+ D  +      ++ F+VD DAGL+LRATLSW DP A++ S  QL+++LDL V +PDGT Y MW  +  D  NVNERVIV A DVV     T  W V V   +  V D+ S+SLVV GAISP
Sbjct:   88 RLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESH-DEAEEEARSSGLD----RLSARHGARMRKPNVEGLSVELTPGTLAARS----SEAK--SFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELG--RAAVYSSSYSSDETATIFNGGDFSYYPERRKA---------------------------------GHGTHTAGSAAGATLNDPAETITC-NVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGI-ERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATG-----------IDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVV-----TGTWTVWVWANNL-VTDSQSYSLVVNGAISP 905          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16098.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 733 bits (1893), Expect = 4.830e-247
Identity = 443/919 (48.20%), Postives = 576/919 (62.68%), Query Frame = 1

Query:   10 KLEGFLGARAVKPVSSTATHGACFMVTASPAEA-AQLSSWEDF--TTFGAFPSALKIAPGLLDHGSCDAPAAARDATTTTSDCSAGRLSTTHGASMQADSAHGLMVELSPGTLPSHGGGDGDEAKGDEFIQELLKSLSSPSLDLHAMTCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAGNDVLLVAGLDHLLYDGREVSGASEE-EAAELRMACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLGHGD-GTEVEHGYYFDFVASSNPSFTSSSESRS-----------------------MFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGS-------SSGM--PFVSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCADTSEVIGCAGGCITESSTGSSEADYGE------DINRLCPAFG------CSGDQCLPDDTADTLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTSLDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGG-DS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSNGKLKFNFNVDPDAGLELRATLSWNDPAASSLSGDQLINDLDLRVYAPDGTKYCMWDDNCWDTSNVNERVIVPADVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISP 2613
            +L+ FL   AV+ VS +   GACF  +AS A+A A +   E F   +F  FPSALK+APGL+DH         +D  TTT      RLS  HGA M+  +  GL +EL+PGTL S         + + FI + L  L   S+DLH+   WSD  M GG+ L    GA+R R+WS+AA++V+E SE+   +P  +C W +V VHHA  DVLLV+GLDHLL+ GR   G S+  E+AEL +AC+MGLVSF AS+ EVL+V     +  LNA+ARA IQSA+V  TPLTDAGLDG+G+V+Q+ID+GV+E++CF  H + G  V HG+YF+     NP  TSSS S                             EF GGDF+   DRRK+IQYI+LI  D  +GS       S G   P++ AD F  D + GHGTH A +VAG+T+++PAEL+SC DT E + C G C+ +      + D         D++RLCP F          ++CL DD A+TL+ +GGMAQGAK+A  D FY ++      GNGLWE CA+A CKIHSNSYG D  C L   DVLYD +M  N E+LL+F+AGN G    R TCT+ SP IAKNVL VG+++SG TRLS T   G G         ID V+ FS YG T D RIKPEV+APGD IYSA+ +G  DS SC L AYAGTS ST + +GA+AM+RQYF + +F+  D+ +RGFCD+   C++FSP + T+KA++INSAN+MGG +EPD  RGFGRIHLEAGMPL G G +ALFV+DS+   +    + ++NF+V+  A L+LR TLSW DP A++ S  QL++DLDL V +P+GT+Y MW     D++NVNERVIV A  V     T  W V +   + T  D  S+SLVV GAISP
Sbjct:   83 RLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHD----KDKETTTG---LDRLSARHGARMRMSNVEGLSIELTPGTLASR------SVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGI-NPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDT-ETMSCVGACVDDDEANDVD-DLATPNVEIVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDG-ERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATP--GLG---------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVE----TGTWSVWIWADNLTT-DVQSYSLVVNGAISP 968          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16098.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 679 bits (1752), Expect = 1.690e-228
Identity = 409/857 (47.72%), Postives = 523/857 (61.03%), Query Frame = 1

Query:   10 KLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSS--WEDFTTFGAFPSALKIAPGLLDHG-SCDAPAAARDATTTTSDCSAGRLSTTHGASMQADSAHGLMVELSPGTLPSHGGGDGDEAKGDEFIQELLKSLSSPSLDLHAMTCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAGNDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLGHGDGTEVEHGYYFDFVASSNPSFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSGMPFVSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCADTSEVIGCAGGCITESSTGSSE---------ADYGEDINRLCPAFGC---SGDQCLPDDTADTLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTSLDVLYDEYMYENQEHLLLFSAGNTGWTTD-RDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDG------RGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQ-GFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSNGKLKFNFNVDPDAGLELRATLSWNDPAASSLSGDQLINDLDLRVYAPDGTKYCMWDDNCW-DTSNVNERVIVPADVV 2505
            +L+  L   AV+PV+ ++THGACF VTAS AEA  +S   +   T+   FPSALK+APG+L+HG S D  +            S GRL+TTHG  M+  +  GL +ELSPGTLP+H        +   FI ELL  L S S+DLH    WSD V   G+HL + AGA+R REWS AATLV++ + + + +P  +C W+ + +H+AGND+LLV+GLDHLL+      G S+EEA ELR+AC+MGLVSF ++R EVL+V+  +    LNA AR  IQSA++   PLTDAGLDG+G+VIQ+ID G++ET+CF    DG EV HG+Y++ +    PS                                           SSS            DD  GHGTH A +  G+TI +PAE  +    +EV GC GGCI    T   +         + Y  DI+RLCP   C   +  +CL DD   TL++HGGMAQGAK+A  D+F    S     GNGLWE C +AGCKIHS S G D  C +   DVLYD++MY N E+L++ +AGN G T D R  CT+ SP I KN L VG+TSSG TR++ T  DG       GYAD      +D V++FS YG T+DGRIKPEVVAPGD IYSA  +G D+ SC L A +GTS +  + +GA+AMVRQYF D SFY AD  +RG+ D  G  C+SFSP + TVKA+LINSAN+MGG +EPD  RGFGRIHLE GMPL G G +ALFVADSS   +       F F+VD +A L+ RATLSW DP ASS SG QL++DLDL V +P G  + MW  +   D+ NVNERVI+ A+ V
Sbjct:   94 RLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDSPYYGLTSISPFPSALKVAPGVLEHGVSLDQASQ-----------SPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAH------SPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIG-SDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEALGEIIPS-------------------------------------------SSSAR----------DDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYAD------VDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDV 873          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16098.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 664 bits (1714), Expect = 5.800e-222
Identity = 411/894 (45.97%), Postives = 531/894 (59.40%), Query Frame = 1

Query:    4 IRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSS--WEDFTTFGAFPSALKIAPGLLDHGSCDAPAAARDATTTTSDCSAGRLSTTHGASMQADSAHGLMVELSPGTLPSHGGGDGDEAKGDEFIQELLKSLSSPSLDLHAMTCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAGNDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLGHGDGTEVEHGYYFDFVASSNPSFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSGMP-------FVSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCADTSEVIGCAGGCITESSTGSSE---------ADYGEDINRLCPAFGCSGDQ---CLPDDTADTLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTSLDVLYDEYMYENQEHLLLFSAGNTGWTTD-RDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDE-LSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGD-SSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQ-GFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSNGKLKFNFNVDPDAGLELRATLSWNDPAASSLSGDQLINDLDLRVYAPDGTKYCMWDDNCW-DTSNVNERVIVPADVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAI 2607
            + +L+  L   AV+ V+ ++THGACF VTAS AEA  +S   +   T+   FPSALK+APG+L+HG       +RD  +     S GRL+TTHG  M+  +  GL +ELSPGTLP+H        +   FI ELL  L S S+DLH    WSD V   G+HL + AGA+R REWS AATLV++ + + + +P  +C W+ + +HHAGND+LLV+GLDHLL+      G S+EEA ELR+AC+MGLVSF +SR EV +V+  +    LNA AR  IQSA++   PLT AGLDG+G+VIQ                                                                VIQYI+LI  D+  G +S          +VS D F  DD   HGTHTA +  G+TI +PAE  +    +EV GC GGCI    T   +         + Y  DI+R+CP  GC  +    CL D    TL++HGGMAQGAK++  DI+ G+  +    GNGLWE C +AGCKIHS S+G DL C +   D+LYD++MY N E+L++ +AGN G   D R  CT+ SP I KN L VG+TSSG TR+  T  DG   ADG    S +D V+ FS YG T+DGRIKPEVVAPGD +YSA  +G D  SC L A +GTS +  + +GA+AMVRQYF D SFY AD  +RG+CD  G  C+SFSP + TVKA+LINSAN+MGG +EPD  RGFGRIHLE GMPL G G +ALFVADSS   +       F F+VD +A L+ RATLSW DP ASS SG QL++DLDL V +P G  + MW  +   D+ NVNERVI+ A+ V   + T    VRV   +  + D+ ++SLVV GA+
Sbjct:   92 LSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDNPYYGLTSISPFPSALKVAPGVLEHG------VSRDQASQ----SPGRLATTHGHLMRKGNVEGLNLELSPGTLPAH------SPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIG-SDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQ----------------------------------------------------------------VIQYINLIKADSVSGPASSTTSGGNRVWWVSPDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGD-RADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYT----VRVS-TNHLLTDSQNYSLVVNGAM 898          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16098.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 628 bits (1620), Expect = 2.240e-205
Identity = 422/1007 (41.91%), Postives = 557/1007 (55.31%), Query Frame = 1

Query:   13 LEGFLGARAV--KPVSSTATHGACFMVTASPAEAAQLSSWED-FTTFGAFPSALKIAPGLLDHGSCDAPAAARDATTTTSDCSAGRLSTTHGASMQA----------------------------------------------DSAHGLMVELSPGTLPSHG----GGDGDEAKGDEFIQELLKSLSSPSLDLHAMTCWS---------DLVMKG-GDHLFSAAGALRGREWSRAATLVNERSESEQ-ETPSQVCGWEQVVVHHAGNDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLGHGDGTE-VEHGYYFDFV---ASSNPSFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDT-----------GD-----------------------GSSSG--MPFVSADSFVSDDDLGHGTHTATTVAGSTISSPA-----ELVSCADTSEVIGCAGGCI-TESSTGSSEA----DY--------GEDINRLCPAFGCSG---DQCLPDDTADTLSEHGGMAQGAKIAAIDIFYGDYSYG-DLAGNGLWETCADAGCKIHSNSYGVDLRCQLTSLDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDE-LSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNG-GDSSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSNGKLKFNFNVDPDAGLELRATLSWNDPAASSLSGDQLINDLDLRVYAPDGTKYCMWDDNCWDTSNVNERVIVPADVVAQFSATDE-----------WEVRVRGRDFTVGDTVSFSLVVTGAISPP 2616
            L   LGA A+  + V S+A HG C + TA+P +A  +   +  F+++ AFPSALK+APGLLDH   D      D          GRL  THG  ++                                                   GL VEL+PG L +       G G  A   E   E  + L S SLD+H  + WS         +   +G GD      G +  REW+RAA ++     +E   T   VC W+ +VV H  +D+L + G+DHLL +GR   G    EA EL++AC+M L+SF ASR EVL+V+       LNAAA   +QSAS+  +P+TDAGLDG+GEVIQI+D G++  +CF    DG E V HGYYF+ +   ASS  S+ +S +        FTGGDF++D  RRK +  +D  +P             GD                        +S+G   P++SA  F  DD  GHGTHTA + AG+ +SSP+     +     +    + C GGCI  +   G  +     DY          D++RLCP   C     + CL DD A+TL +HGG+A+GA++A  DI       G D+AGNG+WE   +AG K+ SNS+G+   C   SLDVL+D++MY NQ+ LLLF+AGN G   D   CT+ +P +AKNVL VG+++SG  RLS TD DG    DGD  +  ID ++ FS +G T D RIKPEVVAPGDMIYSA+G+G G+ +C L  YAGTS ST + +GAAA+VRQYF++ +FY  DVE RGFCD  F C+ F+P A TVKAMLINSAN+MGG  EPD  RGFGR+HLEAGMPL G GD ALFVADS+          +++F VD  AG++LRATLSW DP A++ S  QL++DLDL VY+P G +Y MW D   D+ N NERVIVPA  +   +++D            W VRV  +     +T S+SLVVTGAI PP
Sbjct:   89 LSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQVFSSWAAFPSALKLAPGLLDHPHDDRDGGGGD----------GRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGL-VVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDG--DDWSGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDP-TDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCDS-FGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAIGPP 1080          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16098.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 612 bits (1579), Expect = 1.200e-204
Identity = 350/686 (51.02%), Postives = 447/686 (65.16%), Query Frame = 1

Query:  640 GLDHLLYDGREVSGASEEEAAELRMACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLGHGDGTEVEHGYYFDFVASSN--PSFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPD-------TGDGSSSGMPFVSADSFVSDDDLGHG--------THTATTVAGSTISSPAELVSCADTSEVIGCAGGCI-----TESSTGSSEADYGEDINRLCPAFGCSGDQ---CLPDDTADTLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTSLDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTR-LSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSNGKLKFNFNVDPDAGLELRATLSWNDPAASSLSGDQLINDLDLRVYAPDGTKYCMWDDNCWDTSNVNERVIVPA-DVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISP 2613
            GLDHLL+ GR   G+ EEE+ EL +AC+MGLVS  A R EVL+V+    K  LNAAAR  +QSAS   TPLTDAGLDG+GE+IQ+ID+G++ET+C+     G EV+HGYYFD +A +    S  SS E+ ++F     GGDFSY  +RRK+IQYIDL+NPD       T  GS  G  F+  D F  DD+                 + AG+T+++PAE ++C + ++ + C G CI     T+     S    G D++RLCP +GC       CL DD  +TLS +GGMAQGAK+A  D+FYGDY      GN LWE C +AGCK+HSNS G D  C + S D+L D++MYEN EHLL+F+AGN G   +R TCTI++PGI KN L +G+++SG TR +ST    G           I+ V+EFS +GFT D RIKPEVVAPGD +YSA+ +G D+ SC L AY GTS S  + +GAAAM+RQYF D +FY ADV +RGFC   F C+ FSP A TVKA+LINSAN+MGG +EPD  RGFGR+HLEAGMPL G G++ LFVAD+ D  +      ++ F+VD DAGL+LRATLSW DP +++ S  QL+++LDL V +PDGT Y MW     D  NVNERVIV A DVV     T  W V V      + D+ S+SLVV GAISP
Sbjct:    5 GLDHLLFSGRGAGGSHEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELAQAAVYSSLYSSDETATIFN----GGDFSYYPERRKIIQYIDLVNPDSPYSSFYTSQGSYIGY-FLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITC-NVTDTLSCVGACIDLLDVTDDLVSISHQTGGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGE-ERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATG-----------INTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVV-----TGTWTVWVWANSL-ITDSQSYSLVVNGAISP 666          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16098.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 585 bits (1509), Expect = 9.340e-194
Identity = 345/677 (50.96%), Postives = 436/677 (64.40%), Query Frame = 1

Query:  724 MGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLGHGD-GTEVEHGYYFDFVASSNPSFTSSSESRS------------------------MFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGS-------SSGM--PFVSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCADTSEVIGCAGGCITESSTGSSE---ADYGE--DINRLCPAFG---CSG---DQCLPDDTADTLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTSLDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGG--DSSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSNGKLKFNFNVDPDAGLELRATLSWNDPAASSLSGDQLINDLDLRVYAPDGTKYCMWDDNCWDTSNVNERVIVPADVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISP 2613
            MGLVSF AS+ EVL+V     +  LNAAARA IQSA+V  TPLTDAGLDG+G+V+Q+ID+GV+E++CF  H + G  V HG+YF+     NPS TSSS S                              EF GGDF+   DRRK+IQYI+LI  D  +GS       S G   P++SAD F  DD+ GHGTH A +VAG+T+++PAEL+SC DT E + C G C+ +      +     Y E  D++RLCP F    C G   D+CL DD A+TL+ +GGMAQGAK+A  D FY  +      G GLWE CADA CKIHSNSYG D  C L S DVLYDE+M  N E+LL+F+AGN G    R TCTI SP IAKNVL VG+++SG TRLS T   G           ID V+ FS YG T D RIKPEV+APGD IYSA+ +G     SC L AY GTS ST + +GA+AM+RQYF + +F+  D+ +RGFCD+   C++FSP + T+KA++INSAN+MG  +EP+  RGFGRIHLEAGMPLGG G +ALFV+DS+   +    + ++NF+V+  AGL+LR TLSW DP A++ S  QL++DLDL V +P+GT+Y MW     D+SNVNERVIV AD V     T  W V +   + T  D  S+SLVV GAISP
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGI-NPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDT-ETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDG-ELRRSTCTIGSPAIAKNVLAVGASTSGDTRLSATPGSG-----------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDVE----TGTWSVWIWADNLTT-DVQSYSLVVNGAISP 658