prot_E-siliculosus-1a_M_contig77.15834.1 (polypeptide) Ectocarpus siliculosus Ec864m_EcPH12_78m male

BLAST of mRNA_E-siliculosus-1a_M_contig77.15834.1 vs. uniprot
Match: D8LT18_ECTSI (Valine--tRNA ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LT18_ECTSI)

HSP 1 Score: 1989 bits (5152), Expect = 0.000e+0
Identity = 1044/1104 (94.57%), Postives = 1047/1104 (94.84%), Query Frame = 0

Query:    1 MSGLQEASEKMQEASPAAADANGTETAAAPAQAPEPAXXXXXXXX-------AATAEGGEKKLSKNAMKKLAKSXXXXXXXXXXXXXXXXXXXXXXXXXXAEVIVSYDGPRDGTKKDVSGDMPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFVKRVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGHTKKPKYEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFTERKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAMNAQAGEFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGEKAVDQADLANKERWVVARNEDEAKAKGAAVLGCEPEKVCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRKMSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLDLASELIASLDSAAPRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVVGDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEEPPSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVKSSSEERHNVITKQASDFITLAKASTAVPIMNEEPPEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYVDKVPEAVRETNSKKKAEFEAQKDSVLKAMQQFEGLKI 1097
            MSG QEASEKMQEASPAAADANGT             XXXXXXXX       AATAEGGEKKLSKNAMKKLAKS XXXXXXXXXXXXXXXXXXXXXXXXX EVIV+YDGPRDGTKKDVSGDMPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFV RVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGHTKKPKYEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFT+RKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAMNAQAGEFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGEKAVDQ                                VCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRKMSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLDLASELIASLDSAAPRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVVGDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEE PSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVKSSSEERHNVITKQASDFITLAKASTAVPIMNEEPPEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYV+KVPEAVRETNSKKKAEFEAQKDSVLKAMQQFEGLKI
Sbjct:    1 MSGFQEASEKMQEASPAAADANGTXXXXXXXXXXXXXXXXXXXXXXXXXXTGAATAEGGEKKLSKNAMKKLAKSGXXXXXXXXXXXXXXXXXXXXXXXXXXEVIVTYDGPRDGTKKDVSGDMPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFVNRVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGHTKKPKYEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFTDRKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAMNAQAGEFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGEKAVDQ--------------------------------VCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRKMSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLDLASELIASLDSAAPRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVVGDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEEAPSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVKSSSEERHNVITKQASDFITLAKASTAVPIMNEEPPEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYVNKVPEAVRETNSKKKAEFEAQKDSVLKAMQQFEGLKI 1072          

BLAST of mRNA_E-siliculosus-1a_M_contig77.15834.1 vs. uniprot
Match: A0A835ZF04_9STRA (Valine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZF04_9STRA)

HSP 1 Score: 1365 bits (3534), Expect = 0.000e+0
Identity = 677/1013 (66.83%), Postives = 785/1013 (77.49%), Query Frame = 0

Query:   97 IVSYDGPRDGTKKDVSG-DMPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFVKRVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGHTKKPKYEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFTERKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAMNAQAGEFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGEKAVDQADLANKERWVVARNEDEAKAKGAAVLGCEPEKVCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRK----------MSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLDLASELIASLDSAAPRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVVGDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEEPPSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVKSSSEERHNVITKQASDFITLAKASTA-VPIMNEEPPEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYVDKVPEAVRETNSKKKAEFEAQKDSVLKAMQQFEGLKI 1097
            +  Y+GPRDGTKK +   DMP  YQP YVEAAWQE+WE  GFY+ D  K      DKKF+MVIPPPNVTGSLHLGHALT+A+ED L RWHRM G  TLYVPG DHAGIATQSVVEKKL KDE VTRH LGREEFVKRVW+WK+EYGGKIT QIRKL SSVDW REAFTMD NLSRAV EAF RFHE GL+YRDTRL NWSCALKSAISDIEVDY++LEG+T ++VPGHTK+ KYEFGT+THFAY+VEG +E  ELVVATTRLETMLGDTGVAVHP D RY   HGK+LVHPF  RKIP+VLD VLVDM  GTGAVK+TPAHDPNDY CGKR+NL  I +L  DG+MNA A  FEGM+RYDAR+  E+ L+ KGLLRGK+ +KMRLG+CSRSGD+IEP + PQWYV+C+ MA  AV  V+   L+IEPP+HEKTWY+WL+N+RDWC+SRQLWWGHRIPAYFA  +GE  +D+ D  + +RW VAR++ EA    A  LGC   ++ L QD DVLDTWFSSGLFPFSVFGWP+  ++DLKAF+PTTLLETGMDILFFWVARMVMM L+LT  LPF TV+LH MVRDK GRK          MSK+LGNVIDPLEVI GC LD L  KL+ GNLPAKE+  AK+ Q  DFP+GIPECG DALRFGLLAYTVQGRD+NLDI+RV+GYRNFCNKLWNA RFALTY+T+  P L LA EL A+  + APRD+WILSRLN AVAE NRC   Y FG  T   Y FWLY+LCD+YLELIKPVV D SE N +AR IAQ CLYLCLD+GL+L+HPIMPFVTE+LWQRLPGRGSMG  E  SIM+A YP+A     +P AE     VK AIH  RSLR++YG+ P                     QA DF TLAKA+   V + NEEPP GCAV I+D+   +YVQL+G+VDFD EI KL+ QL+TK++P I  L KKM   GY+ KVPE++R+TN+KK  E +AQ D ++KA+  F+ +K+
Sbjct:   88 VAIYEGPRDGTKKVLGDHDMPEAYQPGYVEAAWQEWWEQCGFYSADTQKGLQAAPDKKFVMVIPPPNVTGSLHLGHALTSAIEDGLTRWHRMRGDVTLYVPGIDHAGIATQSVVEKKLAKDEGVTRHQLGREEFVKRVWQWKEEYGGKITKQIRKLASSVDWEREAFTMDPNLSRAVLEAFVRFHEDGLLYRDTRLVNWSCALKSAISDIEVDYLELEGRTLLSVPGHTKRAKYEFGTLTHFAYKVEGSDE--ELVVATTRLETMLGDTGVAVHPDDPRYTHLHGKSLVHPFNGRKIPIVLDGVLVDMAFGTGAVKVTPAHDPNDYECGKRNNLESINILNPDGSMNAAAAPFEGMLRYDARIACEEELDKKGLLRGKEDNKMRLGICSRSGDVIEPFLAPQWYVRCEGMAAAAVKEVREGRLKIEPPVHEKTWYRWLENARDWCVSRQLWWGHRIPAYFAQRKGE-GLDRNDSTHNDRWFVARSQAEAAVLAAKALGCAEAELELEQDPDVLDTWFSSGLFPFSVFGWPE-QTDDLKAFYPTTLLETGMDILFFWVARMVMMGLQLTGQLPFTTVFLHPMVRDKHGRKPVVCDERGRKMSKSLGNVIDPLEVILGCSLDNLLAKLDAGNLPAKEVATAKKAQTADFPEGIPECGTDALRFGLLAYTVQGRDINLDINRVIGYRNFCNKLWNATRFALTYVTELRPSLALAEELAAN-PNKAPRDKWILSRLNAAVAESNRCFTAYDFGGLTTCLYQFWLYELCDHYLELIKPVVNDKSEANAEARKIAQGCLYLCLDFGLRLLHPIMPFVTEDLWQRLPGRGSMGEGESVSIMLAKYPEAMPAMDDPEAERRYEMVKTAIHTARSLRATYGLTP---------------------QADDFSTLAKAADCGVLVGNEEPPAGCAVQIVDNTLSLYVQLKGLVDFDAEIAKLEGQLETKVKPAIEQLQKKMAMEGYMVKVPESIRDTNTKKLEELQAQADGIIKAIDNFKTMKL 1074          

BLAST of mRNA_E-siliculosus-1a_M_contig77.15834.1 vs. uniprot
Match: A0A6V1PAS2_HETAK (Valine--tRNA ligase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1PAS2_HETAK)

HSP 1 Score: 1313 bits (3399), Expect = 0.000e+0
Identity = 670/1056 (63.45%), Postives = 798/1056 (75.57%), Query Frame = 0

Query:   48 TAEGGEKKLSKNAMKKLAK-----SXXXXXXXXXXXXXXXXXXXXXXXXXXAEVIVSYDGPRDGTKKDVSGDMPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFVKRVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGHTKKPKYEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFTERKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAMNAQAGEFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGEKAVDQADLANKERWVVARNEDEAKAKGAAVLGCEPEKVCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRKMSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLDLASELIASLDSA-APRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVVGDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEEPPSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVKSSSEERHNVITKQASDFITLAKAS--TAVPIMNEEPPEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYVDKVPEAVRETNSKKKAEFEAQKDSVLKAMQQFEGL 1095
            T EGGE+K+SKNA+KK AK                       XXXXXXXXX + +   +    G KKD+   M   Y P  VEAAWQ++WE AG+Y  D  KA   P ++KF +VIPPPNVTGSLHLGHALT A++DTL RWHRM G ATLYVPGTDHAGIATQSVVEKKLKKDEN+TRHDLGRE FV RVW+WK++YGG+I +Q+R LG+SVDW REAFTMD NLSRAVTEAFCRFHE GL+YRDTRL NWSC LKSAISDIEV+++DL GKTY+ VPGHTK   Y+FG +T FAY+VEGG EGEELVVATTRLETMLGDT VAVHP+D RYK  HG+ LVHPF  R+IP+V D VLVDM  GTGAVK+TPAHDPNDY CGKR++L FIT+L  DG +NA    +EG  RYD R ++E+ L+  GL RG+ P+ MRLG+CSRS DIIEP++TPQWYV C  MA  A + V++ EL+I P  HE TW+ WLDN RDWC+SRQLWWGHRIPAYFA+ +GE  VD+A   + +RWVVAR+E  A+   A  LG   ++V L+QDEDVLDTWFSSGLFPFSVFGWP+  ++DLKAF+PTTLLETGMDILFFWVARMVMM L LT  LPF TVYLHAMVRDK+GRKMSK+LGNVIDPLEVI GC L TL  KL  GNLPAKE++KA + Q  DFP+GIPE GADALRFGLLAYTVQGRDVNLDI R+VGYRNFCNKLWNA RFAL+Y+TD +P  DLA  L      A APRDRWILSRL+ A       L+ Y F D T   Y+FWLYDLCD YLELIKPVV D +E N+ AR  AQ+ L++CL+ GL+L+HP+MPFVTEELWQRLPGRG +G +EPPSIMVAPYP A   +ANPA E     +K AIHA RSLR+ YG+ P    +FYV   S E ++ I+ QASD  TLAKA+  T +P   +EPP GCAV+I  D  QV++ LRG+V+F+ E+ KL+ QL  K+ P +  L +KM A GY DKVP+ V+  N +K  E++AQ++++ KA++ F+ L
Sbjct:   36 TKEGGEQKMSKNALKKAAKRAANPKKEKKDPNRWASTKDKDGXXXXXXXXXLKKLEWVNTTPKGDKKDLGQPMLEAYHPRAVEAAWQDWWEKAGYYAPDVQKALRAPPEQKFTIVIPPPNVTGSLHLGHALTTAIQDTLTRWHRMRGDATLYVPGTDHAGIATQSVVEKKLKKDENLTRHDLGREAFVGRVWEWKEQYGGQICSQLRCLGTSVDWGREAFTMDDNLSRAVTEAFCRFHEDGLLYRDTRLVNWSCTLKSAISDIEVEHLDLAGKTYLQVPGHTKHKDYKFGVLTSFAYKVEGGAEGEELVVATTRLETMLGDTAVAVHPEDPRYKHLHGRFLVHPFNGRRIPIVCDPVLVDMSFGTGAVKVTPAHDPNDYECGKRNDLEFITILNEDGTLNANGAPYEGWARYDVRRDMEERLKEMGLFRGEAPNPMRLGLCSRSNDIIEPLLTPQWYVNCSSMAAAACEKVRSGELKILPEFHESTWFMWLDNIRDWCVSRQLWWGHRIPAYFATRKGETGVDRAAPEHGDRWVVARSEGAARFLAAERLGVPEDQVELAQDEDVLDTWFSSGLFPFSVFGWPN-PTDDLKAFYPTTLLETGMDILFFWVARMVMMGLHLTGTLPFTTVYLHAMVRDKYGRKMSKSLGNVIDPLEVINGCALQTLIDKLRAGNLPAKEVEKAAKAQAADFPEGIPEVGADALRFGLLAYTVQGRDVNLDIQRLVGYRNFCNKLWNATRFALSYITDLAPAGDLAEALADPARFALAPRDRWILSRLDAASRAAEAALQGYAFADLTTACYAFWLYDLCDNYLELIKPVVNDAAEANRAARQAAQATLFVCLETGLRLLHPVMPFVTEELWQRLPGRGQLGADEPPSIMVAPYPVARPAWANPALEASYEQIKEAIHACRSLRADYGLTPKQTPDFYVACGSSELYDAISLQASDIRTLAKANQVTVLPA-GQEPPAGCAVNIKSDTLQVHILLRGLVNFEEEVAKLEAQLADKLRPLMENLDRKMNAAGY-DKVPDKVKANNQQKMDEYKAQEENINKAIENFKSL 1088          

BLAST of mRNA_E-siliculosus-1a_M_contig77.15834.1 vs. uniprot
Match: A0A7S4UV17_9STRA (Valine--tRNA ligase n=2 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4UV17_9STRA)

HSP 1 Score: 1240 bits (3208), Expect = 0.000e+0
Identity = 655/1054 (62.14%), Postives = 797/1054 (75.62%), Query Frame = 0

Query:   51 GGEKKLSKNAMKKLAKSXXXXXXXXXXXXXXXXXXXXXXXXXXA--EVIVSYDGPRDGTKKDVSGD-MPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFVKRVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGHTKKP-----KYEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFTERKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAMNAQAGEFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGEKAVDQADLANKERWVVARNEDEAKAKGAAVLGCEPEKVCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRKMSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLDLASELIASLDSAAPRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVVGDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEEPPSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVKSSSEERHNVITKQASDFITLAKASTA-VPIMNEEPPEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYVDKVPEAVRETNSKKKAEFEAQKDSVLKAMQQFEGL 1095
            GGE KLSKNA+KKLAK    XXXXXXXXXXXXXXXXXXXXXX A  + +     P+ G KK++S + M   Y P  VEAAWQ++WEA+GFY CDP+KA     D+KF+MVIPPPNVTGSLHLGHALTAAVEDTL RWHRM GHATLYVPGTDHAGIATQSVVEK L KD  + RHDLGRE+F+++VW+WK EYG +ITTQ+R LGSSVDWSRE FTMD   S+AV EAF +FHE GL+YR  RLGNWSCALKSAISDIEVD ++L G+T++ V  H   P     +YEFGT+T FAY +E  EE   LVVATTRLETMLGDT V +HP+D RYK  HGK ++HPF  R+IP++ D  LVDME GTGAVKITPAHDPNDY CGKRHNL FI VL  DGA+N    +FEGMMRYDAR+ VEKALE KGL +GK+P+KMRL +CSRSGDI+EPMITPQWYV CD MAKRA DAV+N  L+I P  HEKTW+QWLDN RDWC+SRQLWWGH+IPA+FA+ +GE ++ + D+ N +RW+VAR+EDEA  K A +L C  E++ L +DEDVLDTWFSSGLFPFSVFGWPD ++ DLKAF+PT+LLETG+DILFFWVARMVMM LELTD LPF TVYLHAMVRDK GRKMSK+LGNVIDPLEVI GC L++L KKLE GNLP KE+ +AK+ Q+ DFP G+PECG+DALRFGLLAYTVQGRDVNLD+ RVVGYRNFCNKLWNA RFAL +++D  P   L  +L++S    A RD++++SR+     + N  L  YKFGD+   AYS+WL DLCD YLELIKPVV D SE N  +R  AQ+ L++ L+ GL+++HP+MPFVTEELWQRLPGRG++G +EP +IM+A +P+ ++ + N  AE  M      I A RSLRSSY I    + +F+VK +S    +    Q  D ITL +A    V     E  E   + +IDDQS V + L G+VD+  E+KKL+K L +K  P +  L KK+ A GY + V E +++ N +K    + +   + +A+Q+F+ L
Sbjct:   31 GGEGKLSKNALKKLAKGKDKXXXXXXXXXXXXXXXXXXXXXXEAPPKPVFVNKTPK-GQKKNLSAEPMAEAYYPAAVEAAWQDWWEASGFYGCDPSKAEGKSDDEKFVMVIPPPNVTGSLHLGHALTAAVEDTLTRWHRMKGHATLYVPGTDHAGIATQSVVEKMLMKDNGINRHDLGREKFIEKVWEWKKEYGNRITTQLRSLGSSVDWSRERFTMDEMCSKAVVEAFNQFHEKGLLYRAERLGNWSCALKSAISDIEVDSIELAGRTFLEVKTHKGNPDDPKGRYEFGTLTSFAYPIEDSEE--VLVVATTRLETMLGDTAVCIHPEDPRYKHLHGKYVIHPFNNRRIPIIQDAELVDMEFGTGAVKITPAHDPNDYECGKRHNLEFICVLTEDGAINHNGSQFEGMMRYDARIAVEKALEEKGLYKGKEPNKMRLALCSRSGDILEPMITPQWYVNCDGMAKRATDAVRNGTLKIVPDEHEKTWFQWLDNIRDWCVSRQLWWGHQIPAWFATKKGE-SLAKNDMTNNDRWIVARDEDEACEKAAKLLSCAKEEIELERDEDVLDTWFSSGLFPFSVFGWPD-NTSDLKAFYPTSLLETGLDILFFWVARMVMMGLELTDTLPFHTVYLHAMVRDKEGRKMSKSLGNVIDPLEVINGCTLESLLKKLEGGNLPQKEVARAKKDQEADFPDGLPECGSDALRFGLLAYTVQGRDVNLDMKRVVGYRNFCNKLWNATRFALQFVSDFKPTPTLLDDLMSS-GKMAMRDKFMVSRMMNCCEKVNEYLGSYKFGDSQMAAYSYWLDDLCDVYLELIKPVVYDLSESNADSRWAAQATLWIALETGLRILHPMMPFVTEELWQRLPGRGTLGSDEPETIMLASFPECNDAYKNAQAEESMQITMNLIKACRSLRSSYNIGNHVQTQFFVKVASGPAEDAARAQTDDIITLGRAKKVDVNPAESEIHESVGIVVIDDQSTVLMDLTGIVDYAAEVKKLEKTL-SKTTPALKSLEKKIAAKGYEENVNEELKKANEEKLEGLKLKAAEIEEAIQKFKRL 1077          

BLAST of mRNA_E-siliculosus-1a_M_contig77.15834.1 vs. uniprot
Match: A0A7S1FW38_9STRA (Valine--tRNA ligase n=1 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1FW38_9STRA)

HSP 1 Score: 1224 bits (3166), Expect = 0.000e+0
Identity = 648/1072 (60.45%), Postives = 764/1072 (71.27%), Query Frame = 0

Query:   24 TETAAAPAQAPEPAXXXXXXXXAATAEGGEKKLSKNAMKKLAKSXXXXXXXXXXXXXXXXXXXXXXXXXXAEV---------IVSYDGPRDGTKKDVSG-DMPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFVKRVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGHTKKPK-----YEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFTERKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAM-----NAQAGEFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGEKAVDQADLANKERWVVARNEDEAKAKGAAVLGCEPEKVCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRKMSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLDLASELIASLDSAAPRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVVGDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEEPPSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVK-SSSEERHNVITKQASDFITLAKASTAVPIMNEEPPEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYVDKVPEAVRETNSKK 1074
            T  AAAPA  P+PA           A  GEKKLSK  +K+LAK                    XXXXXXXA           + S   P  G KKD++   M   Y P  VE+ W EYWE +GFY+CDPA AA+    +KF MVIPPPNVTGSLHLGHALT AVEDTL RWHRM G AT+YVPGTDHAGIATQSVVEK L K E   RHDLGR+ FV++VW+WK EYGGKIT Q+R LGSSVDWSRE FTMD     AVTEAF RFHESGL+YRDTRLGNWSCALKSAISDIEV+ ++LEG+T++ VP H   PK     YEFGT+T F+Y V  G EGE L VATTRLETMLGDT VAVHP D RYK  HGK L HPF +RKIP++ D  LVDM  GTGAVK+TPAHDPNDY CGKR+NL FITV+   GAM       +  EF GMMRYDAR+ VEKAL++KGL  GK P+KMRL  CSRSGDI+EPMITPQWYV C+ MAKRA DAV++ +L+I P  HEKTW+QWLDN RDWC+SRQLWWGHRIPA+FA+ +GEK V + D+    RW+VAR+ED A+ K AA+L C   +V LS+DEDVLDTWFSSGLFPFSVFGWP+  ++D+KAF+PTTLLETG DILFFWVARMVMM L+LTDVLPFKTVYLHAMVRDK GRKMSK+LGNVIDPLEVI GC L TL+ KL+ GNLPAKE+ +AK  Q  DFP GIPECG+DALRFGLLAYTVQGRDVNLDI RVVGYR FCNKLWNA RFAL ++TD  P   L  +L+ S  + A RD++++SRL             YKFGDA   +Y  W+ ++CD YLELIKPVV D +E N   R  AQ  L++ L+ GL+L+HP+MP+VTEELWQRLPGRG++G EEP SIM+APYP+  E++ +P AE  M      + A RSLRSSY I     A+FYVK +   +    I  Q  D  TL +AS+    +N E P+   + I+DDQ  +Y+ L G+VDF  E+ KLQK L  K  P +  L  KM+A GY +KV   ++  N +K
Sbjct:   37 TPVAAAPA--PDPA-----------APPGEKKLSKKELKRLAKGKGKKEKKEKPASWGAPGAKXXXXXXXASASDAAALATYMSSLPPPGSGGKKDMAALPMLPAYHPAAVESRWNEYWERSGFYSCDPATAAARSEAEKFTMVIPPPNVTGSLHLGHALTCAVEDTLARWHRMSGRATMYVPGTDHAGIATQSVVEKMLAKTEGKNRHDLGRKAFVEKVWEWKKEYGGKITNQLRFLGSSVDWSRERFTMDDTCGAAVTEAFVRFHESGLLYRDTRLGNWSCALKSAISDIEVESLELEGRTFLNVPNHPGNPKDELGRYEFGTITSFSYPVIDGREGEVLTVATTRLETMLGDTAVAVHPDDPRYKHLHGKFLQHPFFQRKIPIITDGTLVDMNFGTGAVKVTPAHDPNDYECGKRNNLEFITVIDEGGAMCKVIAGEECTEFGGMMRYDARIAVEKALDAKGLYGGKKPNKMRLATCSRSGDILEPMITPQWYVNCNSMAKRAADAVRDGDLKILPAEHEKTWFQWLDNIRDWCVSRQLWWGHRIPAWFATQKGEKGVSKNDMEYSSRWIVARDEDAARVKAAALLKCPEGEVDLSRDEDVLDTWFSSGLFPFSVFGWPN-QTDDMKAFYPTTLLETGSDILFFWVARMVMMGLQLTDVLPFKTVYLHAMVRDKDGRKMSKSLGNVIDPLEVINGCTLATLNSKLDNGNLPAKEVVRAKADQASDFPDGIPECGSDALRFGLLAYTVQGRDVNLDIKRVVGYRQFCNKLWNATRFALQFVTDFEPTASLLHDLM-SQGNLAVRDKYMISRLMNGCGAVVDHFTNYKFGDAQMASYQLWMDNICDVYLELIKPVVYDKTEDNAARRRAAQGTLWVALETGLRLLHPMMPYVTEELWQRLPGRGTLGAEEPESIMLAPYPERVEKYRDPQAEASMEVTMGIVRACRSLRSSYQINHKDLAKFYVKVAPGSDAEKYIVAQTDDIQTLGRASSVS--VNVEVPDSVGIVIVDDQCTLYMDLAGLVDFQAELAKLQKNLG-KTLPSVKNLATKMQAAGYEEKVKAEIKAQNVEK 1090          

BLAST of mRNA_E-siliculosus-1a_M_contig77.15834.1 vs. uniprot
Match: B7FQC5_PHATC (Valine--tRNA ligase n=3 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7FQC5_PHATC)

HSP 1 Score: 1223 bits (3164), Expect = 0.000e+0
Identity = 622/1052 (59.13%), Postives = 763/1052 (72.53%), Query Frame = 0

Query:   52 GEKKLSKNAMKKLAKSXXXXXXXXXXXXXXXXXXXXXXXXXXAEVIVSYDGPRDGTKKDVSGDMPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFVKRVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGHTKKP-----KYEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFTERKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAMNAQAGEFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGEKAVDQADLANKERWVVARNEDEAKAKGAAVLGCEPEKVCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRKMSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLDLASELIASLDSAAPRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVVGDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEEPPSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVKSSSEERHNVITKQASDFITLAKASTA-VPIMNEEPPEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYVDKVPEAVRETNSKKKAEFEAQKDSVLKAMQQFEGLKI 1097
            GEKKLSKN +KKLAK                           + V  +   P+ G KKD+S  M A Y P  VEAAWQ++WE  G+Y+CDP  A   P D+KF+MVIPPPNVTGSLHLGHALTAAVEDTL RWHRM GHATLYVPGTDHAGIATQSVVEK L K E  +RHDLGREEFVK+VW+WK +YG KIT Q+R LGSSVDWSRE FTMD  LS+AV EAF RFHE GL+YR  RLGNWSCALKSAISDIEVD+++LEG+T++ V  H   P     +YEFGT+T FAY +E  EE  ++VVATTRLETMLGDT VAVHP D RY   HGK L+HPF  R+IP+V D  LVDM  GTGAVKITPAHDPNDY CGKRH L FIT+L  DG++N     F GMMRYDAR+ VE AL+ KGL +GK+P+KMRLG+CSRSGDI+EPMITPQWYV CD MAKRA DAV+NKEL I P   EKTW+ WLDN +DWC+SRQLWWGH+IPA+FA+ +GE ++++ D+AN +RWVVAR+ +EA  K A +LGC    + + +DEDVLDTWFSSGLFPFSV GWPD D+ DLKAF+PT+LLETG+DILFFWVARMVMM LELTD LPF TV+LHAMVRDK G+KMSK+LGNVIDPLEVI GC L +L ++LE GNLPAKE++++K+  +++FP GIPECG+DALRFGL+AY VQGRD+NLD+ RVVG+R FCNKLWNA RFAL ++ D +P   L  +L+AS    A RD++++SRL  AV   N     Y+FGDA Q AY+ W+ DLC+ YLELIKPVV D S  N   R  AQ+ L++ ++ GL+L+HP+MPFV+EELWQRLPGRG++G  EP +IM+APYP+    + N A E  M    A ++A RSLR SY I    +  F+V  S    H V+  Q  D  TL KAS   + +   + PE     I++DQ  V + L+G+VD+ VEI +LQK L + + P I+ L  KM   GY + VP  +++ N +K      +K  + +A+  FE L +
Sbjct:   22 GEKKLSKNQLKKLAKGKDKKKKDKPQWNAPSKEKVKAPVATTSFVNTT---PK-GEKKDLSAPMDAAYHPSAVEAAWQDWWEKCGYYSCDPKDAVDRPVDEKFVMVIPPPNVTGSLHLGHALTAAVEDTLTRWHRMKGHATLYVPGTDHAGIATQSVVEKMLMKSEGKSRHDLGREEFVKKVWEWKKDYGSKITNQLRSLGSSVDWSRERFTMDEMLSKAVVEAFNRFHEKGLLYRADRLGNWSCALKSAISDIEVDFIELEGRTFLDVKTHKGNPNDPNGRYEFGTLTSFAYPIEDSEE--QIVVATTRLETMLGDTAVAVHPDDPRYTHLHGKHLIHPFNGRRIPIVCDKELVDMSFGTGAVKITPAHDPNDYECGKRHELEFITMLTADGSINENGAPFTGMMRYDARIAVEDALKEKGLFKGKEPNKMRLGLCSRSGDILEPMITPQWYVNCDGMAKRATDAVRNKELTILPEEQEKTWFHWLDNIKDWCVSRQLWWGHQIPAWFATKKGE-SLEKNDMANNDRWVVARSAEEALEKAAKLLGCPAGDISIERDEDVLDTWFSSGLFPFSVMGWPD-DTSDLKAFYPTSLLETGLDILFFWVARMVMMGLELTDTLPFHTVFLHAMVRDKEGKKMSKSLGNVIDPLEVINGCSLASLQERLEGGNLPAKEVERSKKNNELEFPDGIPECGSDALRFGLMAYMVQGRDINLDVKRVVGFRLFCNKLWNATRFALQFVADFTPTPTLLDDLMAS-GKMATRDKFMISRLMKAVEAVNDFFSSYRFGDAQQAAYALWIEDLCNTYLELIKPVVYDMSVNNIDNRWAAQATLWIAMETGLRLLHPMMPFVSEELWQRLPGRGTLGKTEPETIMLAPYPETHNSYKNEAVEQSMMNTMAVVNACRSLRQSYNIANKVQTHFFVNVSGLALHAVLD-QLDDIKTLGKASAIDINLSPADTPETVGTAIVNDQLTVLIDLQGLVDYKVEIGRLQKNLRSTL-PTISTLEMKMATDGYTENVPNDLQKANLEKLDSLLKKKCDLEEAIANFERLAL 1062          

BLAST of mRNA_E-siliculosus-1a_M_contig77.15834.1 vs. uniprot
Match: B5YM58_THAPS (Valine--tRNA ligase n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B5YM58_THAPS)

HSP 1 Score: 1218 bits (3152), Expect = 0.000e+0
Identity = 598/987 (60.59%), Postives = 747/987 (75.68%), Query Frame = 0

Query:  115 MPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFVKRVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGHT-----KKPKYEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFTERKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAMNAQAGEFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGEKAVDQADLANKERWVVARNEDEAKAKGAAVLGCEPEKVCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRKMSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLDLASELIASLDSAAPRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVVGDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEEPPSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVKSSSEERHNVITKQASDFITLAKASTAVPIMNEEP-PEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYVDKVPEAVRETNSKKKAEFEAQKDSVLKAMQQFEGL 1095
            M   Y P  VE+AWQ++WEA+GFY+CDP+ A + P  +KF+MVIPPPNVTGSLHLGHALTAAVEDTL RWHRM GHATLY+PGTDHAGIATQSVVEK + K  N TRHDLGRE F+ +VW+WK EYG +ITTQ+R+LGSSVDWSRE FTMD   S+AV EAF RFHE GL+YR  RLGNWSCALKSAISDIEVDY+DL+G+T++ V  H       K +YEFG +T FAY +E  EE  +LVVATTRLETMLGDT VA+HP+D RY   HGK +VHPF  RKIP+V D  LVDM  GTGAVKITPAHDPNDY CGKRHNL FITVL  DGA+N    +FEGMMRYDAR+ VE+AL+ KGL +GK+P+KMRLG+CSRSGDI+EPMITPQWYV C  MAKR+ DAV+N EL+I P  HEKTW+QWLDN RDWC+SRQLWWGH+IPA+FA+ +GE+ V + D+ + +RW+VAR+E++A  K   +LGC  +++ L +DEDVLDTWFSSGLFPFSV GWP+ +++D KAF+PT+LLETG+DILFFWVARMVMM L+LTD LPF TVYLHAMVRDK GRKMSK+LGNVIDPLEVI GC L+TL +KLE GNLP KE+ +AK+ Q+ DFP+GIPECG+DALRFGLLAYTVQGRDVNLD+ RVVGYR FCNKLWNA RFAL +++D  P  ++  EL+ S    A RD++++SRL       N C   YKFGD+   AYS+W+ D+CD YLELIKPVV D S+ NK  R  AQ+ L++ ++ GL+++HP+MPFVTEELWQRLPGRG++G  EP SIM+A +P+ ++ F N  AE  M      I A RSLR  Y I       F+VK ++ +  +    Q  D  TL KAS     ++E+   +   + ++D+ + V + ++G+VD++ EIKKL+KQL +K E  +  L  KM +PGY D V + V+ +N ++    + +   + +A+  F+ L
Sbjct:    1 MADAYHPEAVESAWQDWWEASGFYSCDPSTAIAKPDSEKFVMVIPPPNVTGSLHLGHALTAAVEDTLTRWHRMKGHATLYIPGTDHAGIATQSVVEKMIMKQSNQTRHDLGREAFISKVWEWKSEYGNRITTQLRRLGSSVDWSRERFTMDDMCSKAVVEAFNRFHEDGLLYRAERLGNWSCALKSAISDIEVDYIDLDGRTFLEVKTHKGNKNDAKGRYEFGVLTSFAYPIEDSEE--QLVVATTRLETMLGDTAVAIHPEDPRYTHLHGKCVVHPFNGRKIPIVCDKELVDMSFGTGAVKITPAHDPNDYECGKRHNLEFITVLTPDGAINHNGSQFEGMMRYDARIAVEEALKEKGLYKGKEPNKMRLGLCSRSGDILEPMITPQWYVNCSDMAKRSTDAVRNGELKILPVEHEKTWFQWLDNIRDWCVSRQLWWGHQIPAWFATKKGEEGVLKTDMKSNDRWIVARSEEDAYDKAEKLLGCSRDEIILERDEDVLDTWFSSGLFPFSVLGWPE-ETDDFKAFYPTSLLETGLDILFFWVARMVMMGLQLTDKLPFHTVYLHAMVRDKDGRKMSKSLGNVIDPLEVINGCTLETLLEKLEGGNLPPKEVARAKKDQEADFPEGIPECGSDALRFGLLAYTVQGRDVNLDMKRVVGYRLFCNKLWNATRFALQFVSDFQPTANILEELMGS-GKMAIRDKFMISRLMACSETVNDCFANYKFGDSQMAAYSYWMNDVCDVYLELIKPVVYDKSDANKDNRWAAQATLWVAIEAGLRILHPMMPFVTEELWQRLPGRGTLGNTEPQSIMLAEFPECNKAFRNMEAEESMEITMNIIRACRSLRQQYNIANKVLTHFFVKVATGQPEDAAKSQTDDIKTLGKASIVDINLDEDAIAKSVGIVVVDEATTVLMDIKGLVDYNAEIKKLEKQL-SKSEGPLQQLEMKMVSPGYDDNVSDEVKASNMERVDALKKKIADIQEAVANFKNL 982          

BLAST of mRNA_E-siliculosus-1a_M_contig77.15834.1 vs. uniprot
Match: A0A5D6Y5N2_9STRA (Valine--tRNA ligase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6Y5N2_9STRA)

HSP 1 Score: 1217 bits (3148), Expect = 0.000e+0
Identity = 602/991 (60.75%), Postives = 731/991 (73.76%), Query Frame = 0

Query:  106 GTKKDVSGDMPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFVKRVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGHTKKPKYEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFTERKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAMNAQAGEFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGEKAVDQADLANKERWVVARNEDEAKAKGAAVLGCEPEKVCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRKMSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLD-LASELIASLDSAAPRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVVGDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEEPPSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVKSSSEERHNVITKQASDFITLAKASTAVPIMNEEPPEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYVDKVPEAVRETNSKKKAEFEAQKDSVLKAMQQFEGL 1095
            G KKD+   M A Y P+ VEAAWQ +WE  GF+ C   +  +   D++F+MVIPPPNVTGSLHLGHALT A++D L RWHRMLGHATL+VPGTDHAGIATQSVVEK+L KDE  +RHDLGR+ F+ RVW WKD+Y  +I  Q R +G SVDWSR  F+MDAN SRAV  AFC+  +  LIYR TR+ NWSC LK+A+S+IEVDYVD++ +T+MAVPGH    KYEFG +  F Y VEGG  GE++VVATTRLETMLGDTGVAVHP D RY   HGK +VHPF  R+IP+VLD  LVDM  GTGAVKITPAHDPNDY CGKRH L FI VL  DGA+N   GEF+G+MRYDARV VE+AL +KGL  GK  +KMRLG+CSRSGD+IEP++ PQW++ CD+MA  A+ AV+NKEL I P  HEKTW++WL+N+RDWCISRQLWWGHRIPAYFA V GE   D    AN  RWV   +ED+A+AK A  LG  P+ + L QDEDVLDTW S+GLFPF+VFGWP   S D   F+PT LLETG DILFFWVARMV +  +LT  LPF+TVYLHAMVRDK+GRKMSK+LGNV+DPLE++ GC L  L  KLE GNLP KE++KAK+GQ+ DFP GIPECGADALRFGLLAYT QGRD+NLDI+R+VGYRNFCNKLWNA RFAL  L D     D LA+ ++A+   +A RDR+ILSRLN  +  CNR    Y FG+ T V Y+FWLYDLCD YLEL KPV+      ++ A+  AQ  LY+CL+YGL+L+HP+MPFVTEELWQRLPG  +       SI V  YPQ    + NPAAE DM  VK AIHA RS+R++YG+V S + EF++K S      V+  Q  DF TLAKA  A  +++ EPP+GCAVH ++++ Q+YVQL G+VD   EI KL+K+L  K+ P +  L KK     Y  KVPE+VRE  + K   F  +++++  A+  F+ L
Sbjct:   91 GEKKDLKAPMAAAYNPVAVEAAWQAWWEQQGFFACSDEQIQAAKPDERFVMVIPPPNVTGSLHLGHALTVAIQDALTRWHRMLGHATLWVPGTDHAGIATQSVVEKRLLKDEGQSRHDLGRDAFLARVWAWKDQYESRIHHQFRSVGCSVDWSRAYFSMDANCSRAVRHAFCQLWDEQLIYRATRMINWSCRLKTALSEIEVDYVDIDKRTFMAVPGHDPARKYEFGVLASFGYPVEGGAAGEQIVVATTRLETMLGDTGVAVHPDDPRYAHLHGKFVVHPFDGRRIPIVLDRELVDMAFGTGAVKITPAHDPNDYECGKRHGLAFINVLTDDGAINENGGEFQGLMRYDARVAVERALAAKGLFYGKADNKMRLGLCSRSGDVIEPLVKPQWFINCDQMAADAMAAVRNKELTILPEAHEKTWFRWLENTRDWCISRQLWWGHRIPAYFARVAGEPWADPNSDANAARWVAGVSEDDARAKAARQLGVSPDAIVLEQDEDVLDTWVSAGLFPFAVFGWPSA-SPDFANFYPTDLLETGSDILFFWVARMVFLGQKLTGQLPFRTVYLHAMVRDKYGRKMSKSLGNVVDPLEIVGGCSLADLLAKLESGNLPPKEVEKAKKGQQADFPNGIPECGADALRFGLLAYTQQGRDINLDINRLVGYRNFCNKLWNATRFALASLDDGFEGGDALAASVLANAVVSA-RDRFILSRLNATIEACNRAFAAYDFGELTNVLYAFWLYDLCDVYLELTKPVLA-ADAADQAAKRTAQQTLYVCLEYGLRLLHPLMPFVTEELWQRLPGARAAA-----SITVVAYPQPVAAWRNPAAEADMELVKDAIHAARSVRAAYGLVNSVRPEFFIKCSDAAVERVVAAQLDDFCTLAKAGDARTLVDAEPPKGCAVHSVNEKLQLYVQLAGLVDAASEISKLEKKLQ-KMAPAVESLQKKRSKDDYAAKVPESVREAEAAKLEAFTKEQNAIASAIADFQRL 1072          

BLAST of mRNA_E-siliculosus-1a_M_contig77.15834.1 vs. uniprot
Match: H3GAQ3_PHYRM (Valine--tRNA ligase n=27 Tax=Peronosporaceae TaxID=4777 RepID=H3GAQ3_PHYRM)

HSP 1 Score: 1211 bits (3134), Expect = 0.000e+0
Identity = 590/991 (59.54%), Postives = 745/991 (75.18%), Query Frame = 0

Query:  106 GTKKDVSGDMPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFVKRVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGHTKKPKYEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFTERKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAMNAQAGEFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGEKAVDQADLANKERWVVARNEDEAKAKGAAVLGCEPEKVCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRKMSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLDLASELIASLDSAAPRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVV-GDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEEPPSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVKSSSEERHNVITKQASDFITLAKASTAVPIMNEEPPEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYVDKVPEAVRETNSKKKAEFEAQKDSVLKAMQQFEGL 1095
            G KK +   M A Y P+ VEAAWQ++WEA+GF++C  A+  S   D++F+MVIPPPNVTGSLHLGHALT A++D L RWHRMLGHATL+VPGTDHAGIATQSVVEK+L K+E  +RHDLGRE F++RVW WK++Y  +I  Q R +G SVDW R+ F+MD N SRAV  AFC+  E GL+YR TR+ NWSC LK+A+S+IEVDY+D++ +T +AVPGH  + KYEFG +T F Y VEG +E  +++VATTRLETMLGD+GVAVHP+D RY   HGK ++HPF  R+IP+VLD VLVDM  GTGAVKITPAHDPNDY CGKRH+L FI VL  DGA+N    EF GMMRYDAR+ VEKALE+KGL  GK+ +KMRLG+CSRSGD+IEP++ PQW+V CD+MAK A+ AV NK+L+I P  HEKTW++WL+N RDWCISRQLWWGHRIPAYFA V+GE  +D+       RW    ++++A  K AA LG   +++ L QDEDVLDTW S+GLFPF+VFGWP+ D+ D + F+PT LLETG DILFFWVARMV M  +LT  LPFKT+YLH+MVRDK+GRKMSK+LGNV+DPLE+I GC LD L KKLE GNLPAKE++KAK+GQK+DFP+GIPECGADALRFGLLAYT QGRD+NLDI R+VGYRNFCNKLWNAVRFA++ L       +  ++ + +      RDR+ILSRLN A+  CN     Y+FG+ T   Y+FWLYDLCD YLEL+KPV+ GD +E    A+  AQ  LY+CL++GL+L+HP MPFVTEELWQRLPG+ S       SI +APYP   E + NP  E++M  VK  IHA RS+R+ YG+  + + +F++K    +   V+  Q  DF TLAKA  +  I++EE P+GCA+H ++D+ Q++V L G+VDF  EI KL+K+L TKI P +  L KKM    Y  KVPE VRE ++ KK   E ++  + +A+ +F+ L
Sbjct:   82 GDKKTMDEPMLAAYNPVAVEAAWQDWWEASGFFSCSDAQIQSAKPDERFVMVIPPPNVTGSLHLGHALTVAIQDALTRWHRMLGHATLWVPGTDHAGIATQSVVEKRLLKEEGKSRHDLGREAFLERVWDWKNQYESRIHHQFRSVGCSVDWDRKYFSMDDNCSRAVRHAFCQLWEEGLVYRATRMINWSCKLKTALSEIEVDYIDIDKRTKLAVPGHNPERKYEFGVLTSFGYPVEGSDE--QIIVATTRLETMLGDSGVAVHPEDPRYTHLHGKFVIHPFNGRRIPIVLDPVLVDMSFGTGAVKITPAHDPNDYECGKRHDLEFINVLTDDGAINENGSEFAGMMRYDARIAVEKALEAKGLYHGKEDNKMRLGICSRSGDVIEPLVKPQWFVNCDQMAKDAMAAVTNKDLKILPETHEKTWFRWLENIRDWCISRQLWWGHRIPAYFARVKGEAFIDKNAEDAGNRWFAGTSDEDALKKAAAKLGVAEDQIELEQDEDVLDTWVSAGLFPFAVFGWPN-DTGDFENFYPTDLLETGYDILFFWVARMVFMGQKLTGKLPFKTIYLHSMVRDKYGRKMSKSLGNVVDPLEIIAGCTLDDLFKKLENGNLPAKEVEKAKKGQKLDFPQGIPECGADALRFGLLAYTQQGRDINLDIGRLVGYRNFCNKLWNAVRFAMSNLEGGFSAAENVADTVLANAVVGSRDRYILSRLNCAIKVCNDSFAAYEFGEMTNALYNFWLYDLCDVYLELLKPVISGDDAE----AKLTAQQTLYVCLEFGLRLLHPSMPFVTEELWQRLPGKRSTA-----SITIAPYPLPMESWTNPEVESNMELVKEVIHAARSVRAEYGLANNVRPKFFIKCIDNDVKAVMEAQLDDFSTLAKAGDSKCIVDEEAPQGCAMHSVNDKVQIFVLLAGLVDFANEIAKLEKKL-TKIAPSLASLEKKMGREDYATKVPENVREADAAKKVALETEEAGIHRAIAEFKRL 1059          

BLAST of mRNA_E-siliculosus-1a_M_contig77.15834.1 vs. uniprot
Match: A0A7S4AC49_9STRA (Valine--tRNA ligase n=1 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4AC49_9STRA)

HSP 1 Score: 1208 bits (3126), Expect = 0.000e+0
Identity = 620/1059 (58.55%), Postives = 766/1059 (72.33%), Query Frame = 0

Query:   46 AATAEGGEKKLSKNAMKKLAKSXXXXXXXXXXXXXXXXXXXXXXXXXXAEVIVSYDGPRDGTKKDVSG-DMPATYQPLYVEAAWQEYWEAAGFYTCDPAKAASMPADKKFIMVIPPPNVTGSLHLGHALTAAVEDTLCRWHRMLGHATLYVPGTDHAGIATQSVVEKKLKKDENVTRHDLGREEFVKRVWKWKDEYGGKITTQIRKLGSSVDWSREAFTMDANLSRAVTEAFCRFHESGLIYRDTRLGNWSCALKSAISDIEVDYVDLEGKTYMAVPGH---TKKPK--YEFGTMTHFAYRVEGGEEGEELVVATTRLETMLGDTGVAVHPKDERYKKFHGKTLVHPFTERKIPVVLDDVLVDMELGTGAVKITPAHDPNDYLCGKRHNLPFITVLGLDGAMNAQAG-EFEGMMRYDARVEVEKALESKGLLRGKDPHKMRLGVCSRSGDIIEPMITPQWYVKCDRMAKRAVDAVKNKELRIEPPMHEKTWYQWLDNSRDWCISRQLWWGHRIPAYFASVEGE-KAVDQADLANKERWVVARNEDEAKAKGAAVLGCEPEKVCLSQDEDVLDTWFSSGLFPFSVFGWPDVDSEDLKAFFPTTLLETGMDILFFWVARMVMMSLELTDVLPFKTVYLHAMVRDKFGRKMSKTLGNVIDPLEVIYGCDLDTLHKKLEVGNLPAKEMQKAKEGQKMDFPKGIPECGADALRFGLLAYTVQGRDVNLDISRVVGYRNFCNKLWNAVRFALTYLTDASPPLDLASELIASLDSAAPRDRWILSRLNVAVAECNRCLEEYKFGDATQVAYSFWLYDLCDYYLELIKPVVGDTSEGNKKARSIAQSCLYLCLDYGLKLIHPIMPFVTEELWQRLPGRGSMGPEEPPSIMVAPYPQADERFANPAAETDMSAVKAAIHAGRSLRSSYGIVPSAKAEFYVKSSSEERHNVITKQASDFITLAKASTA-VPIMNEEPPEGCAVHIIDDQSQVYVQLRGMVDFDVEIKKLQKQLDTKIEPQITMLVKKMEAPGYVDKVPEAVRETNSKKKAEFEAQKDSVLKAMQQFEGL 1095
            A  AEGG +KLSKN +KKLAK                            +       P+ G KKD+S       Y P  VEAAWQE+WEA+GFY  D A A   P ++KF+MVIPPPNVTGSLHLGH LTAAVEDTL RWHRM GHATLYVPGTDHAGIATQSVVEK L K EN  RHDLGREEFVK++W+WK+EYG +IT+Q+R LGSSVDWSRE FTMD  LS+AV EAF RFHE GL+YR  RLGNWSCALKSAISDIEVD++DLEG+T++ V  H   +K PK  YEFG +THFAY VE  EE   LVVATTRLETMLGDT VA+HP+D RY   HGK +VHPF+ RKIP+VLD VLVDM  GTGAVKITPAHDPNDY CGKRH L FIT+L  +GA+N   G EFEG+MRYDAR+ +E+AL+ KGLL+GK+P+KMRLG+CSRSGDI+EPMITPQWYV C+ MAKR+ DAV+N EL+I P  HEKTW+QWLDN RDWC+SRQLWWGH+IPA+FA+ +GE + + + D+AN +RW+VAR+ ++AK K  A LGC  E++ L +DEDVLDTWFSSGLFPFSV GWPD +++DLKAF+PT+LLETG+DI+FFWVARMVMM LELTD LPF TV+LHAMVRDK GRKMSK++GNVIDPLEVI GC L  L  +L+ GNLPAKE+ +AK+    +FP GIPECG+DALRFGLLAYTVQG+D+NLD+ RVVGYRNFCNK+WN+ RFAL YL D  P   L  +L++S    + RD++++SRL   V + +  L EY FG+A Q  Y  WL D+C+ YLELIKPVV  + E  K AR  AQ+ L++ L+  L+ +HP+MPF+TEELWQRLPGRG++G +E  SIM+A +P  ++ + + A+E  M+     + A RSLRSSY I P     F++K + EE  N    Q  D +TL   S   +   + + PE     I++D   V V L+G VDF  E+ +L K L  K +  +  L KK+ A GY   VP+ +++ N +K    + +   +  A+  FE L
Sbjct:   15 APAAEGGGQKLSKNQLKKLAKGKGKKEKKEKPQWGTGKKKETKKKEVKPKEPFVNPTPK-GEKKDMSKIPFADAYDPQAVEAAWQEWWEASGFYKGDAAAAMGRPQEEKFVMVIPPPNVTGSLHLGHGLTAAVEDTLTRWHRMKGHATLYVPGTDHAGIATQSVVEKMLMKSENKNRHDLGREEFVKKIWEWKNEYGNRITSQLRHLGSSVDWSRERFTMDDMLSKAVVEAFNRFHEKGLLYRANRLGNWSCALKSAISDIEVDHIDLEGRTFLEVKTHKGSSKDPKGRYEFGVLTHFAYPVEDSEE--VLVVATTRLETMLGDTAVAIHPEDTRYTHLHGKNVVHPFSGRKIPIVLDPVLVDMAFGTGAVKITPAHDPNDYECGKRHKLEFITILTAEGAINHHGGKEFEGLMRYDARIAIEEALDKKGLLKGKEPNKMRLGLCSRSGDILEPMITPQWYVNCEDMAKRSTDAVRNGELKIIPADHEKTWFQWLDNIRDWCVSRQLWWGHQIPAWFATTKGEGETISKNDMANNDRWIVARSMEDAKEKAMAKLGCTEEELVLERDEDVLDTWFSSGLFPFSVMGWPD-NTDDLKAFYPTSLLETGLDIIFFWVARMVMMGLELTDTLPFHTVFLHAMVRDKEGRKMSKSIGNVIDPLEVIGGCSLQALQDRLDSGNLPAKEVARAKKNNIAEFPDGIPECGSDALRFGLLAYTVQGKDINLDVKRVVGYRNFCNKMWNSTRFALMYLNDFQPTPTLLDDLMSS-GKMSIRDKFMISRLMKGVEDVDTGLTEYTFGNAQQAGYQLWLNDMCNVYLELIKPVVQSSDEEKKDARWAAQATLWVALETQLRGLHPMMPFITEELWQRLPGRGTLGEKEKTSIMLAEFPVVNDSYKDEASEASMALTLDIVAACRSLRSSYNIAPKNLTHFFIKMTDEEHANAARAQTDDIMTLGNGSQVDINPPDTDIPETVGTMIVNDSLTVLVDLKGNVDFKTEVGRLTKNLG-KAKGPMQQLQKKIAADGYQVNVPDDLKKANDEKLLGLQKKCADIELAIANFERL 1067