prot_E-siliculosus-1a_F_contig1384.2286.1 (polypeptide) Ectocarpus siliculosus Ec863f_EcPH12_90f female

BLAST of mRNA_E-siliculosus-1a_F_contig1384.2286.1 vs. uniprot
Match: D8LBA4_ECTSI (Bifunctional dihydrofolate reductase-thymidylate synthase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LBA4_ECTSI)

HSP 1 Score: 999 bits (2583), Expect = 0.000e+0
Identity = 533/582 (91.58%), Postives = 535/582 (91.92%), Query Frame = 0

Query:   32 MASSAAVASPSTSRSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGTTTATTATTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPASASEVDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPADGRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVEFSFDEYESVNHSEAAAXXXXXXXXXXXXXXXXXXXXXXXXXPGGDQVAATGVETPAAXXXXXXXXXXXXXXXXXGLASTAPAPQEEAESGPGNEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIKKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 613
            MASS AVASPSTSRSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSG       T DKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPA+ASEVDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPADGRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVE SFDEYESVNHSEAAA                         P GDQVAATGVETPAAXXXXXXXXXXXXXXXXX          EEAE GPGNEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECI+KIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV
Sbjct:    1 MASSVAVASPSTSRSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGXXXXXXXT-DKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPAAASEVDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPADGRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVELSFDEYESVNHSEAAAAMAAAEGDKENASAASVVGSKRKSSPDGDQVAATGVETPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXEEAEGGPGNEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIQKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 581          

BLAST of mRNA_E-siliculosus-1a_F_contig1384.2286.1 vs. uniprot
Match: A0A835Z035_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z035_9STRA)

HSP 1 Score: 656 bits (1692), Expect = 3.300e-230
Identity = 342/570 (60.00%), Postives = 398/570 (69.82%), Query Frame = 0

Query:   45 RSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGTTTATTATTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPASASE-VDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPADGRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVEFSFDEYESVNHSEAAAXXXXXXXXXXXXXXXXXXXXXXXXXPGGDQVAATGVETPAAXXXXXXXXXXXXXXXXXGLASTAPAPQEEAESGPGNEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIKKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 613
            R FVV+VAATAGSLGIGK G +PW+LA DMAYFK+ TS    +            K+NAV+MGRKTW+SIP+RFRPL GR NVVL+RNP AR  L LP DV++A SL +AL  LS     + V K+FVIGGG VY EA+  E C++V LT+V  PADG+  +CDT FP LDP +FKLV +G+ Q E G+++ F+ YE  + S A A                              V AT                          +S+A      A +   N+EE QYL+LV+DI+DNG  RGDRTGTGTLSKFG QMRFSLR  RFPLLTTKRVFWRGVAEELLWFVAGCT+A  L+ + IHIWDGNGSR FLD  GL  REEGDLGPVYGFQWRHFGA+Y D   DY G+GVDQLA+CI KIK +P+DRRI++SAWNP DL  MALPPCHMFCQFYVA GELSCQMYQRSADMGLGVPFNIASY+LLT +VAQ CGLK GDF+HTIGDAHVYVNHVDALREQ+KR PRPFP L ++   TDIDSFE K   ++GY+P   IKM+MAV
Sbjct:    6 RDFVVVVAATAGSLGIGKGGGIPWKLAGDMAYFKKVTSACDIAG-----------KVNAVVMGRKTWESIPQRFRPLPGRCNVVLTRNPRARQELVLPDDVIIARSLEDALSCLSTQDMRDKVHKIFVIGGGQVYKEALGMEQCKQVHLTTVEAPADGKQFECDTFFPPLDPASFKLVSRGDKQSENGIDYRFNVYERQDSSAAPASGSF------------------------SSVGATN----------------------GSRSSSAKDTSGAAIAEEPNDEERQYLDLVQDIIDNGALRGDRTGTGTLSKFGAQMRFSLRGGRFPLLTTKRVFWRGVAEELLWFVAGCTDAGALQAKNIHIWDGNGSRAFLDSRGLEHREEGDLGPVYGFQWRHFGAKYVDFRTDYKGQGVDQLADCIHKIKNSPDDRRIIMSAWNPCDLELMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTCMVAQVCGLKPGDFIHTIGDAHVYVNHVDALREQIKRVPRPFPKLRLHPARTDIDSFEMKDFTLEGYNPMNAIKMQMAV 518          

BLAST of mRNA_E-siliculosus-1a_F_contig1384.2286.1 vs. uniprot
Match: A0A4D9D1L8_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D1L8_9STRA)

HSP 1 Score: 620 bits (1599), Expect = 5.410e-216
Identity = 329/572 (57.52%), Postives = 385/572 (67.31%), Query Frame = 0

Query:   45 RSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGTTTATTATTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPAS-ASEVDKVFVIGGGSVYAEAVASELCEKVLLTSVTPP--ADGRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVEFSFDEYESVNHSEAAAXXXXXXXXXXXXXXXXXXXXXXXXXPGGDQVAATGVETPAAXXXXXXXXXXXXXXXXXGLASTAPAPQEEAESGPGNEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIKKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 613
            R F ++VAATA + GIGK G LPW L  DM +FKR T           A T+  DKINAVIMGR+TWQSIPE+FRPL  R NVVLSRNP  R+ L LP+ V +A SL  AL LL+  + AS V+KVFVIGG +VY EAV SE CE +  TS+     A   F DCD HFP +    + LV       +  + F F  YE ++   +A                           GG     +G                                        GN+EE QYL LV++I+++G RRGDRT TGTLSKFG Q+RFSLR+E FPLLTTKRVFWRGVAEELLWF++G T+AN LKE+ I IWDGN SRE+LD LGL+ REEGDLGPVYGFQWRHFGAEY+DMHADYTGKG+DQLA  I  IK NPEDRRI++SAWNPADLGKMALPPCHMFCQFYVA+GELSCQMYQRSADMGLGVPFNIASYALLTRLVAQ CGLKAG+F+HTIGDAHVY+NHVDAL+EQL R PR FP L IN  +  IDSF ++   ++GY P++++KMKMAV
Sbjct:   26 RGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLT-----------ACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLNLPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVISEACEAIEFTSIEEKRGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADSPV-----------------------GGGPSKVSGAA--------------------------------------GNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKHIDSFTFEDFTIEGYLPHESLKMKMAV 525          

BLAST of mRNA_E-siliculosus-1a_F_contig1384.2286.1 vs. uniprot
Match: A0A7S2WWT1_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WWT1_9STRA)

HSP 1 Score: 613 bits (1582), Expect = 5.880e-213
Identity = 352/598 (58.86%), Postives = 406/598 (67.89%), Query Frame = 0

Query:   47 FVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGTTTATTATTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPASASE-VDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPADGRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVEFSFDEYESVNHSEAAAXXXXXXXXXXXXXXXXXXXXXXXXXPGGDQVAATGVETPAAXXXXXXXXXXXXXXXXXGLA--------STAPAPQ--------------EEAESGPG--------NEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIKKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 613
            F +IVAATA SLGIG+ G LPWRL  DMA+FKR T            +     + NAVIMGRKT+QSIP RFRPL GR NVV+SRNP  R  L LP +VL+A SL +ALD L  +   + V +V+VIGGGS+Y EAVA E C  +L+T V       F+DCDTHFPA+D   F L+       +KGV  SF+ +E V                                 G  +  A G  +P A XXXXXXXXXXXXXXXX           +TA APQ               E   GPG           EMQYL L++DI+DNGV RGDRTGTGT+SKFG QMRFSLR + FPLLTTK+VFWRGVAEELLWFV+G TNA +L+++ I IWDGN SREFLDG GL  REEGDLGPVYGFQWRHFGA Y DMHA+Y GKGVDQL + I  IK+ P DRRI++SAWNPADL  MALPPCHMFCQFYVA GELSCQMYQRSADMGLGVPFNIASY+LLTR++AQ CGLKAGDF+HTIGDAHVY+NHVDALREQL R PRPFP L IN D+++IDSF++    V+GY P+ TIKMKMAV
Sbjct:    5 FDIIVAATASSLGIGRQGKLPWRLGVDMAFFKRAT-----------LSAGLPGQSNAVIMGRKTYQSIPARFRPLNGRVNVVISRNPEIRAELDLPAEVLVASSLDDALDQLGSSKVGQAVSQVYVIGGGSIYDEAVAHEACRHLLVTMVHSE---EFEDCDTHFPAIDTARFSLLSSSAPFTDKGVVCSFNVFERV---------------------------------GSLRCFANGPASPVAGXXXXXXXXXXXXXXXXXXXXXXXXXXXATAEAPQLTPSEILDGLLEKPVEVPRGPGVNLVGSMAPHPEMQYLALIQDIIDNGVVRGDRTGTGTVSKFGAQMRFSLRGDEFPLLTTKKVFWRGVAEELLWFVSGSTNAKLLQDKKIKIWDGNASREFLDGRGLHHREEGDLGPVYGFQWRHFGARYEDMHANYDGKGVDQLMQAINTIKSRPNDRRIIVSAWNPADLDLMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTRMMAQVCGLKAGDFIHTIGDAHVYLNHVDALREQLARAPRPFPKLRINPDKSEIDSFDFSDFTVEGYKPHGTIKMKMAV 555          

BLAST of mRNA_E-siliculosus-1a_F_contig1384.2286.1 vs. uniprot
Match: A0A7S2MDM9_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2MDM9_9STRA)

HSP 1 Score: 601 bits (1550), Expect = 2.210e-208
Identity = 351/575 (61.04%), Postives = 407/575 (70.78%), Query Frame = 0

Query:   45 RSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGTTTATTATTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPASASE-VDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPADGRFDDCDTHFPALDPDTFKLVKKGE---AQEEKGVEFSFDEY--ESVNHSEAAAXXXXXXXXXXXXXXXXXXXXXXXXXPGGDQVAATGVETPAAXXXXXXXXXXXXXXXXXGLASTAPAPQEEAESGPGNEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIKKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 613
            R+F VIVAATA + GIG+ G LPW++  DMA+FKR T            T      +NAVIMGRKT+QSIP RFRPL GR NVV+SRNP  R  L LP DVL+AGSL++AL ++   + ++ V +VFVIGGGS+Y EAV S+LC +V++T+V       F+DCD  FP+L  D + L          EE  V F+  E   +     E   XXXXXXXXXXXXXXXXXXXX         Q   T    P +                   AST        ES   +EE MQYLNL+ DI+D+GV+RGDRTGTGTLSKFGVQMRFSLR    PLLTTKRVFWRGVAEELLWFV+G TNA  L+++ I IWDGNGSREFLD  GL  REEGDLGPVYGFQWRHFGA+YTDMHADY+G+GVDQLA+ I  IK NP DRRI++SAWNPA L +MALPPCHMFCQFYVA+G LSCQMYQRSADMGLGVPFNIASYALLTRLVA+ C L AG+F+HTIGDAHVY+NHVDALR QL R PRPFP L IN ++ DIDSF Y+ L V+GYSP+ TIKMKMAV
Sbjct:    2 RTFDVIVAATASTFGIGRRGRLPWKIGVDMAFFKRAT-----------LTCDMDGMMNAVIMGRKTYQSIPARFRPLQGRVNVVISRNPNLRQELELPDDVLVAGSLSDALAMIGEGTLNDRVSEVFVIGGGSIYEEAVKSQLCSRVVMTTVHSTG---FEDCDVSFPSLPSDEYILTSASAPVTTSEELVVNFNVFERIKKKTKEDEXXXXXXXXXXXXXXXXXXXXXXXELNALLKTSQAPMTSQLLPPSS------------------ASTL------VESMAAHEE-MQYLNLIRDIIDHGVQRGDRTGTGTLSKFGVQMRFSLRNNVLPLLTTKRVFWRGVAEELLWFVSGSTNAKDLQDKNIRIWDGNGSREFLDSRGLGHREEGDLGPVYGFQWRHFGAQYTDMHADYSGQGVDQLAKAIDAIKNNPTDRRILVSAWNPAALDEMALPPCHMFCQFYVADGALSCQMYQRSADMGLGVPFNIASYALLTRLVARVCDLDAGEFIHTIGDAHVYLNHVDALRTQLTRIPRPFPRLVINEEKRDIDSFSYEDLTVEGYSPHGTIKMKMAV 537          

BLAST of mRNA_E-siliculosus-1a_F_contig1384.2286.1 vs. uniprot
Match: F0YJX9_AURAN (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YJX9_AURAN)

HSP 1 Score: 599 bits (1544), Expect = 6.910e-208
Identity = 325/564 (57.62%), Postives = 369/564 (65.43%), Query Frame = 0

Query:   50 IVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGTTTATTATTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPASASEVDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPADGRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVEFSFDEYESVNHSEAAAXXXXXXXXXXXXXXXXXXXXXXXXXPGGDQVAATGVETPAAXXXXXXXXXXXXXXXXXGLASTAPAPQEEAESGPGNEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIKKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 613
            IVAA AGS GIGK+G LPW+LA DM YFK+ TS             A   K NA +MGRKTW SIP +FRPL GR+NVVLSRNP AR+ L LP DV +  SL  A+D L  A  +++ +VFVIGGG VYA A+A   C KVLLTS+   A G F DCD  FP      F     GEA  E GV + F+                                               +  PA                       AP P  E  + P N EE QYL+L+ +ILD GVRRGDRTGTGT+S FG +M FSLR +RFPLLTTKRVFWRGVAEEL+WFV+G TN  +L ++GIHIWDGNGSREFLD  GL  REE DLGPVYGFQWRHFGA+Y DMH DY G+GVDQLA C++ IK +P  RRIVLSAWNPADL +MALPPCHMFCQFYVA GELSCQMYQRSADMGLGVPFNIASYALLTR+VAQA GLK GDFVH IGDAHVY NHVDAL+ QL+R+PRPFPTL+IN  +T ID FE+    V+GY PY  I MKMAV
Sbjct:   20 IVAAVAGSYGIGKDGTLPWKLAGDMKYFKKVTSA------------APEGKTNACVMGRKTWLSIPPKFRPLGGRKNVVLSRNPNAREELGLPPDVFVCPSLDAAVDAL--AGEADLGEVFVIGGGQVYASALADARCTKVLLTSIDDAA-GAFADCDAVFPDPAKAGFASAASGEALVENGVAYRFET----------------------------------------------LTRPAKKPPLRESN-----------GPPAPPPANEKPAAP-NHEEKQYLDLIAEILDRGVRRGDRTGTGTISIFGARMSFSLRNDRFPLLTTKRVFWRGVAEELVWFVSGSTNGKLLADKGIHIWDGNGSREFLDRRGLGHREEMDLGPVYGFQWRHFGAKYVDMHKDYAGEGVDQLAACVETIKQDPNSRRIVLSAWNPADLDEMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRMVAQATGLKPGDFVHVIGDAHVYSNHVDALKVQLQREPRPFPTLTINPAKTGIDDFEFADFTVEGYKPYPKIDMKMAV 510          

BLAST of mRNA_E-siliculosus-1a_F_contig1384.2286.1 vs. uniprot
Match: A0A7R9UC25_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=2 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9UC25_9STRA)

HSP 1 Score: 585 bits (1509), Expect = 5.070e-202
Identity = 312/575 (54.26%), Postives = 383/575 (66.61%), Query Frame = 0

Query:   45 RSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGTTTATTATTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLR--LPKDVLLAGSLTEALDLLSPAS--ASEVDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPADGRFDD--CDTHFPALDPDTFKLVKKGEAQEEKGVEFSFDEYESVNHSEAAAXXXXXXXXXXXXXXXXXXXXXXXXXPGGDQVAATGVETPAAXXXXXXXXXXXXXXXXXGLASTAPAPQEEAESGPGNEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIKKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 613
            R FVV+VAATA SLGIGK+G LPWR+  DM++FK+ TS            T      NAVIMGRKTW+SIP +FRPL+GR N+VLSR+P   ++LR  LP+ V +A SL EAL +++P      +V+ VFVIGGGSVYA+A+ S  C++++LT VT P +G  +D  CDT  P +D   +  V +   Q ++   +SF  YE      AA                                                           G       PQ +A + P + E  QYL+LV DI+++GV RGDRTGTGTLSKFGVQMRFSLR+  FPLLTTKRVFWRG+AEELLWF+ GCTNANVL E+ IHIWDGNGSREFLD  GLT+RE GDLGPVYGFQWRHFGA+Y DMHADY G+GVDQL + I  +K NPE RR +L+AWNP+DL KMALPPCHM  QFYVA+GELSC +YQRS DMGLGVPFNIASYALLTR++AQ  GL+ GDFVH IGDAHVY+NHV+ L+EQL+R+P PFPTL +N+ +TDIDSFE+    +  Y  ++ +KM MAV
Sbjct:   46 RHFVVVVAATASSLGIGKDGKLPWRIRGDMSFFKKLTSE-----------TRIPGHRNAVIMGRKTWESIPSKFRPLSGRLNIVLSRHP---ESLRGQLPEQVAVASSLEEALAMVAPGGIHCDQVEDVFVIGGGSVYADALRSSYCKRIVLTEVT-PLEGTEEDLGCDTFLPRIDGSAYMRVAESGDQVDENFRYSFVTYERRATGAAATD---------------------------------------------------------GPPQAQAQPQAQARTNPDHAE-WQYLDLVRDIIESGVERGDRTGTGTLSKFGVQMRFSLRDGSFPLLTTKRVFWRGLAEELLWFIQGCTNANVLAEKNIHIWDGNGSREFLDKRGLTDREVGDLGPVYGFQWRHFGAQYRDMHADYAGEGVDQLRQIISDLKANPESRRHILTAWNPSDLPKMALPPCHMMAQFYVADGELSCLLYQRSGDMGLGVPFNIASYALLTRIIAQVTGLQPGDFVHCIGDAHVYLNHVEPLKEQLRREPLPFPTLEVNSKKTDIDSFEFSDFVLHNYRHHRGVKMDMAV 547          

BLAST of mRNA_E-siliculosus-1a_F_contig1384.2286.1 vs. uniprot
Match: A0A8J2SV63_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SV63_9STRA)

HSP 1 Score: 583 bits (1502), Expect = 1.440e-201
Identity = 321/564 (56.91%), Postives = 368/564 (65.25%), Query Frame = 0

Query:   50 IVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGTTTATTATTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPASASEVDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPADGRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVEFSFDEYESVNHSEAAAXXXXXXXXXXXXXXXXXXXXXXXXXPGGDQVAATGVETPAAXXXXXXXXXXXXXXXXXGLASTAPAPQEEAESGPGNEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIKKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 613
            IVAATA  +GIG++GALPWRL  DMAYFKR T            T A   + NAV+MGRKTW SIP++FRPL GR NVVLSRNP AR+ L LP+DV    SL  AL  +   +  +V KVFVIGGG +Y  AV    C +VLLT V  P  G    CD  FP L    F  V     ++E  + +SF        S                                + +    VE P                         P P       P N+EE QYL+L+ +ILD+GV RGDRTGTGT S FG +M+FSLR++ FPLLTTKRVFWRGVAEELLWFV+G TN  +L+E+ IHIWDGNGSREFLD  GL  REE DLGPVYGFQWRHFGA Y DMHADY GKGVDQLAECI++IK +P  RRIVLSAWNPADL +MALPPCHMFCQFYVA GELSCQMYQRSADMGLGVPFNIASYALLTR+VAQ+  LKAGDFVH IGDAHVY+NH+DAL EQLKR PRPFP+L+IN  + DID+FEY    V GY PY  I+M+MAV
Sbjct:   16 IVAATA-QMGIGRDGALPWRLKGDMAYFKRVT------------TEAPASQQNAVVMGRKTWLSIPKKFRPLPGRANVVLSRNPDAREQLDLPEDVACYASLDAALTQMK--AREDVAKVFVIGGGELYKAAVNDARCTEVLLTRVEDPK-GVLPQCDAFFPDLTGTGFTTVAMSPPEKEGDLAYSFQTLTRECSSTKLGDVT-------------------------NSLKEKLVEKPP------------------------PVP-------PVNKEEQQYLDLIREILDSGVVRGDRTGTGTKSIFGARMKFSLRDDVFPLLTTKRVFWRGVAEELLWFVSGATNGKLLQEKNIHIWDGNGSREFLDKRGLPHREEMDLGPVYGFQWRHFGASYEDMHADYAGKGVDQLAECIRQIKGDPNSRRIVLSAWNPADLPEMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRMVAQSTNLKAGDFVHVIGDAHVYLNHIDALEEQLKRAPRPFPSLTINAAKKDIDAFEYDDFTVDGYKPYPKIQMEMAV 507          

BLAST of mRNA_E-siliculosus-1a_F_contig1384.2286.1 vs. uniprot
Match: C1MMA6_MICPC (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Micromonas pusilla (strain CCMP1545) TaxID=564608 RepID=C1MMA6_MICPC)

HSP 1 Score: 570 bits (1470), Expect = 1.830e-196
Identity = 321/593 (54.13%), Postives = 377/593 (63.58%), Query Frame = 0

Query:   39 ASPSTSRSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGTTTATTATTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLR--------------LPKDVLLAGSLTEALDLLSPASASE-VDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPAD--GRFDDCDTHFPALDPDTFKLVKKGEAQEEK-GVEFSFDEYESVNHSEAAAXXXXXXXXXXXXXXXXXXXXXXXXXPGGDQVAATGVETPAAXXXXXXXXXXXXXXXXXGLASTAPAPQEEAESGPGNEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIKKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 613
            AS  T+R F V+VAAT G +GIG +GALPWRL  DMAYFK           + TAT A   K NAVIMGRKTW+SIP +FRPL GR NVVLS++ A  ++                LP  VLL  SL  AL  L     +E V+K+FV+GG  VYAEA+AS  C+ V +T VTPP D    F  CDTH P +D + FKL        EK G +  F  Y + +                                     AATG                       G  S  P     A +     EE+QYL+L+ DI+++G  +GDRTGTGT+SKFG  MRF LR   FPLLTTKRVFWRGVAEELLWFV G TNA  L+++ IHIWDGNGSRE+LD +GL+ REE DLGPVYGFQWRHFGAEYTDMHA+Y GKGVDQLAE I KIK NP DRRI+L+AWNPA L +MALPPCHMFCQFYVA GELSCQMYQRS DMGLGVPFNIASY+LLT ++AQ CGLK GDFVH +GD HVY NHV+ L++QL  +PRPFPTL IN  +TDIDSFE+    ++GY P+  I+MKMAV
Sbjct:    4 ASAPTTRPFQVVVAATKGDMGIGLDGALPWRLPKDMAYFK-----------SVTATVAEPGKRNAVIMGRKTWESIPAKFRPLPGRLNVVLSKSGALVESTGSENAAPANGTPSNVLPPSVLLQPSLEAALATLGAGEHAENVEKIFVVGGAQVYAEAMASPACQAVHITEVTPPKDTPDAFK-CDTHMPPVDMEKFKLYASAPPVNEKEGAKIQFLSYVAKD-------------------------------------AATG------------------KFRIPGSVSLPPG----AVANGVRHEELQYLDLIRDIIESGNVKGDRTGTGTISKFGTSMRFDLRRS-FPLLTTKRVFWRGVAEELLWFVGGRTNAKELQDKDIHIWDGNGSREYLDSVGLSHREEQDLGPVYGFQWRHFGAEYTDMHANYDGKGVDQLAEVIDKIKNNPNDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCGLKPGDFVHVMGDTHVYSNHVEPLKKQLLCEPRPFPTLKINPAKTDIDSFEFSDFTIEGYDPHPKIEMKMAV 524          

BLAST of mRNA_E-siliculosus-1a_F_contig1384.2286.1 vs. uniprot
Match: A0A1Y1HPI3_KLENI (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1HPI3_KLENI)

HSP 1 Score: 568 bits (1465), Expect = 7.210e-195
Identity = 319/590 (54.07%), Postives = 391/590 (66.27%), Query Frame = 0

Query:   45 RSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGTTTATTATTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDT-LRLPKDVLLAGSLTEALDLLSPAS-ASEVDKVFVIGGGSVYAEAVASELCEKVLLTSV----TPPADGRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVEFSFDEYESVNHSEAAAXXXXXXXXXXXXXXXXXXXXXXXXXPGGD----QVAATGVETPAAXXXXXXXXXXXXXXXXXGLASTAPAPQEEAESGPG-----------NEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIKKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 613
            R+F ++VAAT   LGIG+NG+LPW+L  DM +FK+ T+T           T++ DK NAV+MGRKTW SIP +FRPL GR NVVL+R  +  D+    P+ VL+  SL  A+  L+ A  A +V+ VFVIGGG +Y EA+AS LC+ V LT V    TP       +CDT  P +DP  F+       + E  + +SF  Y   +  E                             G +    + AA   E  A    XXXXXXXXXX    G+   A +  + A +GPG             EE+QYL L+++I+  G  +GDRTGTGT+SKFG  MRF+LR+  FPLLTTKRVFWRGV EELLWFVAG TNA +L++RG+HIWDGNGSR +LD +GLT REE DLGPVYGFQWRHFGA+YTDMHADYTG+GVDQLA+ I +IKT PEDRR++LSAWNPADL  MALPPCHMFCQFYVA+GELSCQMYQRS DMGLGVPFNIASY+LLT ++AQ CGL+ GDF+H +GDAHVY NHV+ L+EQL+  PRPFP L +N    DIDSF     E+ GY P+K I MKMAV
Sbjct:   11 RTFQIVVAATQ-DLGIGRNGSLPWKLPGDMRFFKQVTTT-----------TSSPDKRNAVVMGRKTWDSIPPKFRPLPGRLNVVLTRAASKTDSDSAYPEGVLVCASLDSAMSHLASADHADKVETVFVIGGGQIYREAMASPLCDAVHLTEVNTRDTPL------ECDTFMPPVDPSQFRAWSASPPKVENDLRYSFVTYVRTHAPEEPQGVETNGAAVAPSGVVVESTGRVESAGGAELGAGESAAERPERGAEGDGXXXXXXXXXXSAQLGVLGKAKSGSDGA-TGPGAVARLPKSIQEKHEELQYLKLIDEIIREGAHKGDRTGTGTISKFGCSMRFNLRKS-FPLLTTKRVFWRGVVEELLWFVAGSTNARLLQDRGVHIWDGNGSRAYLDSIGLTHREEHDLGPVYGFQWRHFGAKYTDMHADYTGQGVDQLADLIHRIKTKPEDRRLILSAWNPADLQLMALPPCHMFCQFYVADGELSCQMYQRSCDMGLGVPFNIASYSLLTLMIAQVCGLRPGDFIHVLGDAHVYTNHVEPLQEQLRNTPRPFPMLKVNPAVNDIDSFTADDFELIGYEPHKKIAMKMAV 580