mRNA_E-siliculosus-1a_F_contig13759.2244.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig13759.2244.1
Unique NamemRNA_E-siliculosus-1a_F_contig13759.2244.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig13759.2244.1 vs. uniprot
Match: D8LSN8_ECTSI (WD-40 repeat family protein / mitotic checkpoint protein, putative n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LSN8_ECTSI)

HSP 1 Score: 147 bits (370), Expect = 6.220e-42
Identity = 72/74 (97.30%), Postives = 73/74 (98.65%), Query Frame = 1 
Query:   28 QVFTMSVSDMRAVVGTSNRHIWVYDMRSLVEPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYFDTSK 249
            +VFTMSVSDMRAVVGTSNRHIWVYDMRSL EPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYFDTSK
Sbjct:  106 KVFTMSVSDMRAVVGTSNRHIWVYDMRSLAEPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYFDTSK 179
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BLAST of mRNA_E-siliculosus-1a_F_contig13759.2244.1 vs. uniprot
Match: A0A166IB96_DAUCS (Uncharacterized protein n=1 Tax=Daucus carota subsp. sativus TaxID=79200 RepID=A0A166IB96_DAUCS)

HSP 1 Score: 101 bits (251), Expect = 7.410e-24
Identity = 46/81 (56.79%), Postives = 65/81 (80.25%), Query Frame = 1 
Query:    7 GFFHDRNQVFTMSVSDMRAVVGTSNRHIWVYDMRSLVEPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYFDTSK 249
            G ++   +V+++SV+  R V  T+ RH+ VYD+R++ +PEQRR SSLKYQTRCVR +P+Q GYA+ SVEGRVA+E+FDTS+
Sbjct:  139 GTYNQLERVYSLSVNGHRLVAATAGRHVNVYDLRNMSQPEQRRESSLKYQTRCVRCYPNQTGYALSSVEGRVAMEFFDTSE 219
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BLAST of mRNA_E-siliculosus-1a_F_contig13759.2244.1 vs. uniprot
Match: A0A1D2N8I6_ORCCI (Mitotic checkpoint protein BUB3 n=1 Tax=Orchesella cincta TaxID=48709 RepID=A0A1D2N8I6_ORCCI)

HSP 1 Score: 99.8 bits (247), Expect = 2.390e-23
Identity = 47/82 (57.32%), Postives = 65/82 (79.27%), Query Frame = 1 
Query:    1 PHGFFHDRNQVFTMSVSDMRAVVGTSNRHIWVYDMRSLVEPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYFDTS 246
            P G    ++QV+TMSV D   +VGTS+R I V+D+R++ +P Q+R S LKYQTRCVRIFP+++GY + S+EGRVAVEYFD++
Sbjct:  132 PIGVHSQQDQVYTMSVKDEMLIVGTSSRKIIVWDLRNMSQPFQQRDSLLKYQTRCVRIFPNKQGYVLSSIEGRVAVEYFDSN 213
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BLAST of mRNA_E-siliculosus-1a_F_contig13759.2244.1 vs. uniprot
Match: A0A7S1DG33_HEMAN (Hypothetical protein (Fragment) n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1DG33_HEMAN)

HSP 1 Score: 97.4 bits (241), Expect = 5.570e-23
Identity = 44/71 (61.97%), Postives = 58/71 (81.69%), Query Frame = 1 
Query:   25 NQVFTMSVSDMRAVVGTSNRHIWVYDMRSLVEPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYF 237
            ++VFT++V   R VVGT+NRH+ +YD+R L EPEQ R SSLK+QTRC+R FP+  GYA+ S+EGRVA+EYF
Sbjct:  137 DKVFTLAVCQNRVVVGTANRHVLIYDVRKLGEPEQERESSLKFQTRCIRCFPNGLGYALSSIEGRVAMEYF 207
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BLAST of mRNA_E-siliculosus-1a_F_contig13759.2244.1 vs. uniprot
Match: A0A6U4SK10_HEMAN (Hypothetical protein n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A6U4SK10_HEMAN)

HSP 1 Score: 97.4 bits (241), Expect = 1.810e-22
Identity = 44/71 (61.97%), Postives = 58/71 (81.69%), Query Frame = 1 
Query:   25 NQVFTMSVSDMRAVVGTSNRHIWVYDMRSLVEPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYF 237
            ++VFT++V   R VVGT+NRH+ +YD+R L EPEQ R SSLK+QTRC+R FP+  GYA+ S+EGRVA+EYF
Sbjct:  137 DKVFTLAVCQNRVVVGTANRHVLIYDVRKLGEPEQERESSLKFQTRCIRCFPNGLGYALSSIEGRVAMEYF 207
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BLAST of mRNA_E-siliculosus-1a_F_contig13759.2244.1 vs. uniprot
Match: UPI001C276B2B (mitotic checkpoint protein BUB3.1-like isoform X2 n=1 Tax=Salvia splendens TaxID=180675 RepID=UPI001C276B2B)

HSP 1 Score: 96.7 bits (239), Expect = 1.940e-22
Identity = 46/81 (56.79%), Postives = 62/81 (76.54%), Query Frame = 1 
Query:    7 GFFHDRNQVFTMSVSDMRAVVGTSNRHIWVYDMRSLVEPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYFDTSK 249
            G +    +V++MSV   R VV T+ RH+ VYD+R++ +PEQRR SSLKYQTRCVR +P+  GYA+ SVEGRVA+E+FD S+
Sbjct:   88 GTYTQPERVYSMSVVGNRLVVATAGRHVNVYDLRNMSQPEQRRESSLKYQTRCVRCYPNGTGYALSSVEGRVAMEFFDLSE 168
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BLAST of mRNA_E-siliculosus-1a_F_contig13759.2244.1 vs. uniprot
Match: A0A453IMG6_AEGTS (Uncharacterized protein n=1 Tax=Aegilops tauschii subsp. strangulata TaxID=200361 RepID=A0A453IMG6_AEGTS)

HSP 1 Score: 94.4 bits (233), Expect = 1.990e-22
Identity = 43/81 (53.09%), Postives = 62/81 (76.54%), Query Frame = 1 
Query:    7 GFFHDRNQVFTMSVSDMRAVVGTSNRHIWVYDMRSLVEPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYFDTSK 249
            G +    +V++MS+   R VV T+ RH+ +YD+R++ +PEQ+R SSLKYQTRCVR FP+  GYA+ SVEGRV++E+FD S+
Sbjct:  101 GTYAQPERVYSMSLVGNRLVVATAGRHVSIYDLRNMSQPEQKRESSLKYQTRCVRCFPNGTGYALSSVEGRVSMEFFDLSE 181
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BLAST of mRNA_E-siliculosus-1a_F_contig13759.2244.1 vs. uniprot
Match: A0A7T1BYB7_PROCL (Mitotic checkpoint protein BUB3-like n=15 Tax=Decapoda TaxID=6683 RepID=A0A7T1BYB7_PROCL)

HSP 1 Score: 96.3 bits (238), Expect = 2.110e-22
Identity = 45/80 (56.25%), Postives = 61/80 (76.25%), Query Frame = 1 
Query:    7 GFFHDRNQVFTMSVSDMRAVVGTSNRHIWVYDMRSLVEPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYFDTS 246
            G F   N+V+TM +S  + VVGTSNR + V+D+R++   +QRR SSLKYQTRC++ FP+++GY V S+EGRVAVEY D S
Sbjct:   81 GTFQQPNKVYTMGLSGEKLVVGTSNRKVMVWDLRNMGFAQQRRESSLKYQTRCIQCFPNKQGYVVSSIEGRVAVEYLDPS 160
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BLAST of mRNA_E-siliculosus-1a_F_contig13759.2244.1 vs. uniprot
Match: K8Z3F8_NANGC (Cell cycle arrest protein BUB3 n=3 Tax=Monodopsidaceae TaxID=425072 RepID=K8Z3F8_NANGC)

HSP 1 Score: 97.1 bits (240), Expect = 2.460e-22
Identity = 46/73 (63.01%), Postives = 57/73 (78.08%), Query Frame = 1 
Query:   28 QVFTMSVSDMRAVVGTSNRHIWVYDMRSLVEPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYFDTS 246
            +VFT++VS  R VVGTS+R + +YD+R L  P+ RR SSLK+QTRC+RI PD  GY + SVEGRVAVEYFD S
Sbjct:  141 KVFTLAVSGQRLVVGTSDRSVLIYDVRKLSAPDDRRESSLKHQTRCIRIAPDHSGYVLASVEGRVAVEYFDLS 213
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BLAST of mRNA_E-siliculosus-1a_F_contig13759.2244.1 vs. uniprot
Match: A0A7S2RA62_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RA62_9STRA)

HSP 1 Score: 94.7 bits (234), Expect = 2.490e-22
Identity = 45/74 (60.81%), Postives = 57/74 (77.03%), Query Frame = 1 
Query:   28 QVFTMSVSDMRAVVGTSNRHIWVYDMRSLVEPEQRRVSSLKYQTRCVRIFPDQKGYAVGSVEGRVAVEYFDTSK 249
            +VF++ +S  R +VGTS R +  YD+R+L EPE  R SSLKYQTRCVR+FPD  G+AV S+EGRVA+EYFD  K
Sbjct:   28 KVFSLGLSSNRLLVGTSERRLVSYDLRNLKEPEFDRESSLKYQTRCVRVFPDGTGFAVASIEGRVAIEYFDPEK 101
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig13759.2244.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LSN8_ECTSI6.220e-4297.30WD-40 repeat family protein / mitotic checkpoint p... [more]
A0A166IB96_DAUCS7.410e-2456.79Uncharacterized protein n=1 Tax=Daucus carota subs... [more]
A0A1D2N8I6_ORCCI2.390e-2357.32Mitotic checkpoint protein BUB3 n=1 Tax=Orchesella... [more]
A0A7S1DG33_HEMAN5.570e-2361.97Hypothetical protein (Fragment) n=1 Tax=Hemiselmis... [more]
A0A6U4SK10_HEMAN1.810e-2261.97Hypothetical protein n=1 Tax=Hemiselmis andersenii... [more]
UPI001C276B2B1.940e-2256.79mitotic checkpoint protein BUB3.1-like isoform X2 ... [more]
A0A453IMG6_AEGTS1.990e-2253.09Uncharacterized protein n=1 Tax=Aegilops tauschii ... [more]
A0A7T1BYB7_PROCL2.110e-2256.25Mitotic checkpoint protein BUB3-like n=15 Tax=Deca... [more]
K8Z3F8_NANGC2.460e-2263.01Cell cycle arrest protein BUB3 n=3 Tax=Monodopsida... [more]
A0A7S2RA62_9STRA2.490e-2260.81Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig13759contigE-siliculosus-1a_F_contig13759:161..409 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start0
Stop0
Cds size249
Model size249
Exons1
Hectar predicted targeting categoryother localisation
BRITEko00000,ko00001,ko00002,ko03019,ko03036,ko03041,ko04121
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
COG Functional cat.S
EggNOG OGsKOG1036@1,KOG1036@2759
EggNOG free text desc.spindle assembly checkpoint
GOsGO:0000003,GO:0000070,GO:0000075,GO:0000228,GO:0000278,GO:0000280,GO:0000775,GO:0000776,GO:0000777,GO:0000778,GO:0000779,GO:0000780,GO:0000793,GO:0000794,GO:0000819,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005694,GO:0005737,GO:0005829,GO:0006508,GO:0006511,GO:0006807,GO:0006996,GO:0007049,GO:0007059,GO:0007088,GO:0007093,GO:0007094,GO:0007154,GO:0007165,GO:0007275,GO:0007346,GO:0008104,GO:0008150,GO:0008152,GO:0008608,GO:0009056,GO:0009057,GO:0009790,GO:0009792,GO:0009987,GO:0010498,GO:0010564,GO:0010639,GO:0010948,GO:0010965,GO:0016043,GO:0019538,GO:0019941,GO:0022402,GO:0022414,GO:0023052,GO:0030071,GO:0030163,GO:0031145,GO:0031570,GO:0031577,GO:0031974,GO:0031981,GO:0032182,GO:0032501,GO:0032502,GO:0032991,GO:0033036,GO:0033043,GO:0033044,GO:0033045,GO:0033046,GO:0033047,GO:0033048,GO:0033313,GO:0033316,GO:0033597,GO:0034613,GO:0035556,GO:0040020,GO:0043130,GO:0043161,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043632,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044444,GO:0044446,GO:0044454,GO:0044464,GO:0044774,GO:0044779,GO:0045786,GO:0045835,GO:0045839,GO:0045841,GO:0045930,GO:0048285,GO:0048519,GO:0048523,GO:0048856,GO:0050789,GO:0050794,GO:0050896,GO:0051128,GO:0051129,GO:0051179,GO:0051276,GO:0051321,GO:0051445,GO:0051447,GO:0051603,GO:0051641,GO:0051716,GO:0051726,GO:0051783,GO:0051784,GO:0051983,GO:0051985,GO:0065007,GO:0070013,GO:0070727,GO:0071173,GO:0071174,GO:0071704,GO:0071840,GO:0072395,GO:0072413,GO:0072416,GO:0072477,GO:0072480,GO:0072486,GO:0098687,GO:0098813,GO:0140014,GO:1901564,GO:1901565,GO:1901575,GO:1901987,GO:1901988,GO:1901990,GO:1901991,GO:1901993,GO:1901994,GO:1902099,GO:1902100,GO:1902102,GO:1902103,GO:1903046,GO:1903047,GO:1905132,GO:1905133,GO:1905818,GO:1905819,GO:1990298,GO:2000241,GO:2000242,GO:2000816,GO:2001251
KEGG ModuleM00427
KEGG Pathwayko03013,ko04110,ko04111,ko05010,ko05164,ko05166,map03013,map04110,map04111,map05010,map05164,map05166
KEGG TC1.I.1
KEGG koko:K02180,ko:K04532,ko:K14298
Preferred nameBUB3
Seed eggNOG ortholog2880.D8LSN8
Seed ortholog evalue1.6e-33
Seed ortholog score148.3
Taxonomic scopeEukaryota
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927890.1079776-CDS-E-siliculosus-1a_F_contig13759:160..4091622927890.1079776-CDS-E-siliculosus-1a_F_contig13759:160..409Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig13759 161..409 -
1696318724.863501-CDS-E-siliculosus-1a_F_contig13759:160..4091696318724.863501-CDS-E-siliculosus-1a_F_contig13759:160..409Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig13759 161..409 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig13759.2244.1prot_E-siliculosus-1a_F_contig13759.2244.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig13759 161..409 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig13759.2244.1

>prot_E-siliculosus-1a_F_contig13759.2244.1 ID=prot_E-siliculosus-1a_F_contig13759.2244.1|Name=mRNA_E-siliculosus-1a_F_contig13759.2244.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=83bp
PHGFFHDRNQVFTMSVSDMRAVVGTSNRHIWVYDMRSLVEPEQRRVSSLK
YQTRCVRIFPDQKGYAVGSVEGRVAVEYFDTSK
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mRNA from alignment at E-siliculosus-1a_F_contig13759:161..409-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig13759.2244.1 ID=mRNA_E-siliculosus-1a_F_contig13759.2244.1|Name=mRNA_E-siliculosus-1a_F_contig13759.2244.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=249bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig13759:161..409- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CCACACGGTTTTTTTCACGATCGGAACCAGGTCTTCACCATGTCCGTCTC
GGACATGCGCGCCGTCGTCGGTACCTCGAACCGGCACATCTGGGTGTACG
ACATGCGCAGCCTGGTGGAACCGGAGCAGCGGCGCGTCAGCAGCCTCAAG
TACCAGACGCGCTGCGTGAGGATATTCCCGGACCAGAAGGGCTACGCGGT
GGGGTCTGTGGAGGGGAGGGTGGCCGTCGAGTACTTCGACACGTCCAAG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig13759:161..409-

>mRNA_E-siliculosus-1a_F_contig13759.2244.1 ID=mRNA_E-siliculosus-1a_F_contig13759.2244.1|Name=mRNA_E-siliculosus-1a_F_contig13759.2244.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=498bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig13759:161..409- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CCACACGGTTTTTTTCACGATCGGAACCAGGTCTTCACCATGTCCGTCTC
GGACATGCGCGCCGTCGTCGGTACCTCGAACCGGCACATCTGGGTGTACG
ACATGCGCAGCCTGGTGGAACCGGAGCAGCGGCGCGTCAGCAGCCTCAAG
TACCAGACGCGCTGCGTGAGGATATTCCCGGACCAGAAGGGCTACGCGGT
GGGGTCTGTGGAGGGGAGGGTGGCCGTCGAGTACTTCGACACGTCCAAGC
CACACGGTTTTTTTCACGATCGGAACCAGGTCTTCACCATGTCCGTCTCG
GACATGCGCGCCGTCGTCGGTACCTCGAACCGGCACATCTGGGTGTACGA
CATGCGCAGCCTGGTGGAACCGGAGCAGCGGCGCGTCAGCAGCCTCAAGT
ACCAGACGCGCTGCGTGAGGATATTCCCGGACCAGAAGGGCTACGCGGTG
GGGTCTGTGGAGGGGAGGGTGGCCGTCGAGTACTTCGACACGTCCAAG
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