mRNA_E-siliculosus-1a_F_contig10158.161.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig10158.161.1
Unique NamemRNA_E-siliculosus-1a_F_contig10158.161.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig10158.161.1 vs. uniprot
Match: A0A6H5JXQ5_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JXQ5_9PHAE)

HSP 1 Score: 250 bits (638), Expect = 4.390e-79
Identity = 144/156 (92.31%), Postives = 144/156 (92.31%), Query Frame = 1 
Query:    4 NQAELTEEEDSSLFFVQLPTKLPRPLAACQRGKDGASAPVRGKGKXXXXXGGPAVKEEAGESGTGGAAXXXXXXXXXXAAAEKASEIPADDVAFDQGLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV 471
            NQAELTEEEDSSLFFVQLPTKLPRPLAA QRGKDGASAPVRGKGK     GGPAVKEEAGESG G   XXXXXXXX  AAAEKASEIPADDVAFDQGLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV
Sbjct:  245 NQAELTEEEDSSLFFVQLPTKLPRPLAAFQRGKDGASAPVRGKGKGKAT-GGPAVKEEAGESGAGXXXXXXXXXXXKGAAAEKASEIPADDVAFDQGLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV 399
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BLAST of mRNA_E-siliculosus-1a_F_contig10158.161.1 vs. uniprot
Match: D8LHB7_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LHB7_ECTSI)

HSP 1 Score: 245 bits (626), Expect = 1.520e-76
Identity = 147/157 (93.63%), Postives = 148/157 (94.27%), Query Frame = 1 
Query:    4 NQAELTEEEDSSLFFVQLPTKLPRPLAACQRGKDGASAPVRGKGKXXXXX-GGPAVKEEAGESGTGGAAXXXXXXXXXXAAAEKASEIPADDVAFDQGLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV 471
            NQAELTEEEDSSLFFVQLPTKLPRPLAACQRGKDGASAPVRGKGKXXXX  GGPAVKEEAGESG G   XXXXXXXXXX  AEKASEI ADDVAFD+GLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV
Sbjct:  308 NQAELTEEEDSSLFFVQLPTKLPRPLAACQRGKDGASAPVRGKGKXXXXATGGPAVKEEAGESGAGXXXXXXXXXXXXXXXAEKASEIAADDVAFDRGLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV 464
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BLAST of mRNA_E-siliculosus-1a_F_contig10158.161.1 vs. uniprot
Match: A0A835ZEC9_9STRA (RNA polymerase III RPC4-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZEC9_9STRA)

HSP 1 Score: 74.3 bits (181), Expect = 1.430e-12
Identity = 55/154 (35.71%), Postives = 71/154 (46.10%), Query Frame = 1 
Query:   10 AELTEEEDSSLFFVQLPTKLPRPLAACQRGKDGASAPVRGKGKXXXXXGGPAVKEEAGESGTGGAAXXXXXXXXXXAAAEKASEIPADDVAFDQGLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV 471
            A L  EED  +FFVQLPT LPR         +  S P  G G       G          GT              AAA+ + E       +D  L  M PG+IGKL + +SG  +L++ GV+L V  G+P  FVE+   ++ KQKT   FGQV
Sbjct:  290 AALQAEEDEGIFFVQLPTALPRVFPESM---EHPSQPTDGGGD------GXXXXXHGAVGGT--------RAQGAKAAADTSGEADGSP-GYDNSLTTMLPGRIGKLQIRRSGTVQLLLAGVALDVSAGMPCHFVEELTCVNPKQKTMSMFGQV 425
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BLAST of mRNA_E-siliculosus-1a_F_contig10158.161.1 vs. uniprot
Match: A0A7S3UWU6_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UWU6_HETAK)

HSP 1 Score: 67.0 bits (162), Expect = 1.470e-11
Identity = 30/67 (44.78%), Postives = 44/67 (65.67%), Query Frame = 1 
Query:  271 DDVAFDQGLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV 471
            +D   D GLRR+ PG++G++ VH SG+ +L +G     V PGLP  FV+  V++DA+  T C FGQ+
Sbjct:    5 EDPVADGGLRRVAPGRVGRVRVHASGRVKLTLGDTVFDVAPGLPCHFVQDVVAVDAEGGTACFFGQL 71
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BLAST of mRNA_E-siliculosus-1a_F_contig10158.161.1 vs. uniprot
Match: A0A6G0WW31_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WW31_9STRA)

HSP 1 Score: 58.9 bits (141), Expect = 2.680e-7
Identity = 31/73 (42.47%), Postives = 45/73 (61.64%), Query Frame = 1 
Query:  253 ASEIPADDVAFDQGLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV 471
            A+E   DD+ FD+ L  M  G +GK+ +H+SGKA LV+G  +  + PG P +F E+ VSID K++     G V
Sbjct:  244 ATERSQDDL-FDKSLHAMPGGFMGKINIHKSGKAVLVLGDKTFNIAPGHPPTFYEEAVSIDTKEELLSMLGPV 315
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BLAST of mRNA_E-siliculosus-1a_F_contig10158.161.1 vs. uniprot
Match: W4GQG3_9STRA (Uncharacterized protein n=2 Tax=Aphanomyces astaci TaxID=112090 RepID=W4GQG3_9STRA)

HSP 1 Score: 58.5 bits (140), Expect = 3.750e-7
Identity = 33/76 (43.42%), Postives = 44/76 (57.89%), Query Frame = 1 
Query:  244 AEKASEIPADDVAFDQGLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV 471
            A K+SE    +  FD+ L  M  G +GK+ +H+SGKA LV+G  +  + PG P SF E+ VSID K K     G V
Sbjct:  252 ASKSSEN-GTETRFDKSLSAMPGGYLGKINIHKSGKAVLVLGDKTFDLAPGQPPSFYEEVVSIDVKDKLLSMLGPV 326
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BLAST of mRNA_E-siliculosus-1a_F_contig10158.161.1 vs. uniprot
Match: A0A6A5AC38_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A6A5AC38_9STRA)

HSP 1 Score: 57.4 bits (137), Expect = 9.590e-7
Identity = 29/66 (43.94%), Postives = 39/66 (59.09%), Query Frame = 1 
Query:  274 DVAFDQGLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV 471
            +  FD+ L  M  G +GK+ +H+SGKA LV+G  +  + PG P SF E+ VSID K K     G V
Sbjct:  266 ETRFDKSLSAMPGGYLGKINIHKSGKAVLVLGDKTFDLAPGQPPSFYEEVVSIDVKDKLLSMLGPV 331
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BLAST of mRNA_E-siliculosus-1a_F_contig10158.161.1 vs. uniprot
Match: A0A7S3VAP1_9STRA (Hypothetical protein n=1 Tax=Chaetoceros debilis TaxID=122233 RepID=A0A7S3VAP1_9STRA)

HSP 1 Score: 55.8 bits (133), Expect = 3.760e-6
Identity = 29/84 (34.52%), Postives = 44/84 (52.38%), Query Frame = 1 
Query:  241 AAEKASEIPADDVAFDQGLRRMRPGKIGKLYVHQSGKARLVIGG-------VSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV 471
            AA   S        +D  L+    GK GK+ +H+SGKA LV+GG       V +++  GLP  F++Q V+ID    ++   G+V
Sbjct:  420 AAAPTSSTNTSGAGYDDTLKDSAAGKYGKIVIHKSGKAYLVVGGADSKTPQVKMMLTEGLPCGFLQQAVAIDPNNASYVPLGEV 503
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BLAST of mRNA_E-siliculosus-1a_F_contig10158.161.1 vs. uniprot
Match: A0A024TER0_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TER0_9STRA)

HSP 1 Score: 55.1 bits (131), Expect = 6.040e-6
Identity = 31/74 (41.89%), Postives = 43/74 (58.11%), Query Frame = 1 
Query:  250 KASEIPADDVAFDQGLRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKTFCSFGQV 471
            KA    ADD+ FD+ L  M  G +GK+ +H+SGKA LV+G  +  +  G P +  E+ VSID K K F   G +
Sbjct:  252 KAPNHGADDL-FDKSLLSMPGGYLGKINIHKSGKAVLVLGDKTFELAAGQPPACYEEVVSIDVKDKLFSMLGPI 324
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BLAST of mRNA_E-siliculosus-1a_F_contig10158.161.1 vs. uniprot
Match: T1HGK6_RHOPR (Uncharacterized protein n=1 Tax=Rhodnius prolixus TaxID=13249 RepID=T1HGK6_RHOPR)

HSP 1 Score: 52.0 bits (123), Expect = 8.710e-6
Identity = 26/54 (48.15%), Postives = 36/54 (66.67%), Query Frame = 1 
Query:  295 LRRMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQ--GVSIDAKQKT 450
            LR + PG+IGKL + +SGKARL++G V L V PG   +F +    V++D  QKT
Sbjct:   26 LRNLPPGRIGKLQILKSGKARLILGDVKLWVEPGTQTAFKQDLISVNLDPNQKT 79
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig10158.161.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 14
Match NameE-valueIdentityDescription
A0A6H5JXQ5_9PHAE4.390e-7992.31Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LHB7_ECTSI1.520e-7693.63Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A835ZEC9_9STRA1.430e-1235.71RNA polymerase III RPC4-domain-containing protein ... [more]
A0A7S3UWU6_HETAK1.470e-1144.78Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A6G0WW31_9STRA2.680e-742.47Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
W4GQG3_9STRA3.750e-743.42Uncharacterized protein n=2 Tax=Aphanomyces astaci... [more]
A0A6A5AC38_9STRA9.590e-743.94Uncharacterized protein n=1 Tax=Aphanomyces astaci... [more]
A0A7S3VAP1_9STRA3.760e-634.52Hypothetical protein n=1 Tax=Chaetoceros debilis T... [more]
A0A024TER0_9STRA6.040e-641.89Uncharacterized protein n=1 Tax=Aphanomyces invada... [more]
T1HGK6_RHOPR8.710e-648.15Uncharacterized protein n=1 Tax=Rhodnius prolixus ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig10158contigE-siliculosus-1a_F_contig10158:747..2526 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start0
Stop0
Cds size471
Model size471
Exons3
Ec32 orthologEc-24_001300.1
Ec32 ortholog descriptionDNA-directed RNA polymerase III subunit RPC4
Hectar predicted targeting categoryother localisation
Best eggNOG OGNA|NA|NA
Best tax levelBacillariophyta
EggNOG OGs2E6IC@1,2SD7V@2759,2XDZ5@2836
Seed eggNOG ortholog35128.Thaps11342
Seed ortholog evalue4.4e-08
Seed ortholog score64.7
Taxonomic scopeEukaryota
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig10158.161.1prot_E-siliculosus-1a_F_contig10158.161.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig10158 747..2526 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927517.726828-CDS-E-siliculosus-1a_F_contig10158:746..8971622927517.726828-CDS-E-siliculosus-1a_F_contig10158:746..897Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10158 747..897 +
1696318564.2715278-CDS-E-siliculosus-1a_F_contig10158:746..8971696318564.2715278-CDS-E-siliculosus-1a_F_contig10158:746..897Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10158 747..897 +
1622927517.7387178-CDS-E-siliculosus-1a_F_contig10158:1397..15731622927517.7387178-CDS-E-siliculosus-1a_F_contig10158:1397..1573Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10158 1398..1573 +
1696318564.284613-CDS-E-siliculosus-1a_F_contig10158:1397..15731696318564.284613-CDS-E-siliculosus-1a_F_contig10158:1397..1573Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10158 1398..1573 +
1622927517.751611-CDS-E-siliculosus-1a_F_contig10158:2382..25261622927517.751611-CDS-E-siliculosus-1a_F_contig10158:2382..2526Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10158 2383..2526 +
1696318564.2918236-CDS-E-siliculosus-1a_F_contig10158:2382..25261696318564.2918236-CDS-E-siliculosus-1a_F_contig10158:2382..2526Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10158 2383..2526 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig10158.161.1

>prot_E-siliculosus-1a_F_contig10158.161.1 ID=prot_E-siliculosus-1a_F_contig10158.161.1|Name=mRNA_E-siliculosus-1a_F_contig10158.161.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=157bp
QNQAELTEEEDSSLFFVQLPTKLPRPLAACQRGKDGASAPVRGKGKGKGK
AGGPAVKEEAGESGTGGAAAGATSGKGTGAAAEKASEIPADDVAFDQGLR
RMRPGKIGKLYVHQSGKARLVIGGVSLVVHPGLPVSFVEQGVSIDAKQKT
FCSFGQV
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mRNA from alignment at E-siliculosus-1a_F_contig10158:747..2526+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig10158.161.1 ID=mRNA_E-siliculosus-1a_F_contig10158.161.1|Name=mRNA_E-siliculosus-1a_F_contig10158.161.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=1780bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig10158:747..2526+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CAGAACCAAGCCGAGCTCACAGAGGAGGAAGACAGCAGCCTCTTCTTCGT
GCAGTTGCCGACCAAGCTTCCCAGGCCGCTGGCGGCGTGCCAGAGAGGGA
AGGACGGCGCTTCGGCGCCCGTGCGGGGCAAGGGGAAGGGGAAGGGGAAG
GGTGAGCCGGTTGTGGACGGGTGCATGTGTTTTTTTTTTGTATCCAGGCC
GATGTGGGTCAGAATTGTAATTGTTTGTCTTCGCCTTACTCGCGAGATAA
AATGGACGGTTAGCGCTCACATCGCTCTGCCTTTCACCGTGGCATCTGTG
TTGCGTGAAGCCGCAGCGCTGTTCGTGAGCCGTTTATTTTTATGCCCGTC
ATTGCCGGAGCAATGTTTTCGTCAAAGGATAGAGGGTCAAGCGCATGCTT
TGTTGTGGAGACTCCTGCACCAACAACACGAGCAAGTACCACAACAACAG
TTGCACCATCACCACCACTACCAACAGTAGCAGCAGCAGCGACAACAACA
ATCCCAAGAACATCCGAAACTGCTTGGGCTTGGACATCCGCCCAAGAGGT
GTCTCCGCTCCCCTAGTACCCCCCTCCCGTTCACGCTTTTCCCTAGCTTG
ATGTTCGTTTTGGCACCCCCCGTTGTTTTCCCGGTGCTTCCTTTCCGAGA
GCAGGAGGCCCCGCGGTGAAAGAGGAAGCGGGGGAATCGGGTACAGGGGG
AGCAGCTGCCGGGGCTACCAGCGGCAAGGGAACGGGTGCTGCGGCAGAGA
AGGCTTCCGAGATACCGGCAGACGACGTGGCGTTCGATCAGGGGCTCCGG
CGAATGCGCCCTGGCAAGATTGGCAAGGTATGTACCAGCGCCGGCTGGAC
TGCGACCGTTGTCGTCGTCGTTGTTGTTGTTGGCGGTGGTGGTGCTGATC
GGTGCGGGTTTGTTGTTGTTGTTGCTGTTGATATTGGTTTTGATCTGGTC
GTCGAGAGTCGCTTGGCGTCCAACGGGTTGTTCGTGTCATGTCTCGTTAG
TAGAGATGTTTGTTCCGTGGCTTGGCAGGATGTTTTCCCCCGTGCAGCCT
GTGGCTTGCGCTGTAGTTCGCGGGTTTGCCCCGTAGTCGCGTGCCCCGTT
CGCACCATTCGCTGACTTGGCGATGCTAGTGTGCTGCGTCGTAAAATTCA
CACGGCGACACCTCGCTACCCGACATGACCGGAATCGACAGCAGCGAGTG
CTGTCCCATGGGCTACATAACGATGCAAGCACACCAGCCCAAGTACACGT
CAGTTTAGCACAGGATGCGAAGGGTTTGTGATAGTGTGAACCTTCCATGT
GGCGTGAACACGAACACGTACACAACCCCTTTTGTTTTTATTTCGCTCGT
TGCGGGGACTTGACCTGTAAACCTACTCCTCCCCCCCCCACCCCGCTGTC
GAGGACCCCGCGGAATAGAACTCGCTTTTCGTTGACCCTACTAAAACTAG
TATATTCGTCTTGCCCTTCCTAAATTGTAAAGCACGTGCAACCGACACAC
GTACGCACACCTGCCCCGCTTGTCAACCTCCCGCGACTTTGTGGAAAATG
CACGGATGGATCAGCCTGTCGACTACATTATTATTCCACCGCGGGTCCCA
ACACCCTACCCCCCCATCGCCGTTTGCTGTGTACAGCTTTACGTGCACCA
GTCCGGCAAGGCTAGGCTAGTGATTGGAGGCGTGAGCCTAGTGGTGCACC
CAGGATTGCCGGTGTCGTTCGTGGAACAGGGCGTTAGCATCGACGCCAAG
CAAAAGACGTTCTGCTCCTTCGGACAGGTA
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig10158:747..2526+

>mRNA_E-siliculosus-1a_F_contig10158.161.1 ID=mRNA_E-siliculosus-1a_F_contig10158.161.1|Name=mRNA_E-siliculosus-1a_F_contig10158.161.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=942bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig10158:747..2526+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CAGAACCAAGCCGAGCTCACAGAGGAGGAAGACAGCAGCCTCTTCTTCGT
GCAGTTGCCGACCAAGCTTCCCAGGCCGCTGGCGGCGTGCCAGAGAGGGA
AGGACGGCGCTTCGGCGCCCGTGCGGGGCAAGGGGAAGGGGAAGGGGAAG
GCAGAACCAAGCCGAGCTCACAGAGGAGGAAGACAGCAGCCTCTTCTTCG
TGCAGTTGCCGACCAAGCTTCCCAGGCCGCTGGCGGCGTGCCAGAGAGGG
AAGGACGGCGCTTCGGCGCCCGTGCGGGGCAAGGGGAAGGGGAAGGGGAA
GGCAGGAGGCCCCGCGGTGAAAGAGGAAGCGGGGGAATCGGGTACAGGGG
GAGCAGCTGCCGGGGCTACCAGCGGCAAGGGAACGGGTGCTGCGGCAGAG
AAGGCTTCCGAGATACCGGCAGACGACGTGGCGTTCGATCAGGGGCTCCG
GCGAATGCGCCCTGGCAAGATTGGCAAGCAGGAGGCCCCGCGGTGAAAGA
GGAAGCGGGGGAATCGGGTACAGGGGGAGCAGCTGCCGGGGCTACCAGCG
GCAAGGGAACGGGTGCTGCGGCAGAGAAGGCTTCCGAGATACCGGCAGAC
GACGTGGCGTTCGATCAGGGGCTCCGGCGAATGCGCCCTGGCAAGATTGG
CAAGCTTTACGTGCACCAGTCCGGCAAGGCTAGGCTAGTGATTGGAGGCG
TGAGCCTAGTGGTGCACCCAGGATTGCCGGTGTCGTTCGTGGAACAGGGC
GTTAGCATCGACGCCAAGCAAAAGACGTTCTGCTCCTTCGGACAGGTACT
TTACGTGCACCAGTCCGGCAAGGCTAGGCTAGTGATTGGAGGCGTGAGCC
TAGTGGTGCACCCAGGATTGCCGGTGTCGTTCGTGGAACAGGGCGTTAGC
ATCGACGCCAAGCAAAAGACGTTCTGCTCCTTCGGACAGGTA
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