prot_E-siliculosus-1a_F_contig1011.128.1 (polypeptide) Ectocarpus siliculosus Ec863f_EcPH12_90f female

BLAST of mRNA_E-siliculosus-1a_F_contig1011.128.1 vs. uniprot
Match: D7FNM0_ECTSI (Tetrahydrofolate synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FNM0_ECTSI)

HSP 1 Score: 1046 bits (2705), Expect = 0.000e+0
Identity = 561/653 (85.91%), Postives = 574/653 (87.90%), Query Frame = 0

Query:    1 MVESVLRHHGFRTAGLYTSPHLLEVTERVRCNGKPLTKEAFARYFFQVWDRLQDSAARTNT------AT----GTTGIPAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPPPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASQQCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCVVWIEAHRQQXXXXXAGDQAPPPEKIRNTEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWFVEKTATAAASRAKADEVVSSRAAVAVAVTPREIPRSSPXXXXXXXXXXXQKRRCRRVLWFNASHERDVVPLLHMLASARRREPCNGSSGNATNTTNKDDHNDADGDTPLFDDAWFMEVNPGRPSRFKLPTAQEILAPHGMSPAVAAAAATPAGIAGASSVSSGPCXXXXXXXXXXXXXXXXXGEEKADSAGAESGAGAWQRTLEQVWTSLQTRRGGTLSATLTGTAPFFFEKIALICANSQHDGGSEAASVVGDPGVSSRAAASAVDADRHGSGGRGDPSVVATGCGDGGGRVDSEMVEVLCTGSLYAVAAALEAFGAEVE 643
            MVES+LRHHGFRT GLYTSPHLLEVTERVRC+GKPLTKEAFARYFFQVWDRLQD+AA TNT      AT    GTTGI AAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVP PVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVL EASQQCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLC VWIEAHRQQ     AGDQAPP EKI +TE FWASA+VHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWFVEKTATAAASRAKADEV SSRAAVAVAVTP +IPRSSPXXXXXXXXXXX  RRCRRVLWFNASHERDVVPLL MLASARRRE C+GSSGNA           ADGDTPLFD+AWFMEV PGRPSRFKLPTAQEILAPHG+SPAVAAAAAT            GPC                 GEEKAD AGA SGAGAWQRTLEQVWTSLQTRRGGTLSATLTGTAPFFFEK++LICANSQHDGGSEAASVVGDPGV+SR A SAVDAD HGSGGRGD SV ATGCGD GGRVDSE+VEVLCTGSLYAVAAALEAFGAEVE
Sbjct:   80 MVESILRHHGFRT-GLYTSPHLLEVTERVRCDGKPLTKEAFARYFFQVWDRLQDTAAPTNTDDATAAATXXXXGTTGITAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLCEASQQCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCAVWIEAHRQQQQQQHAGDQAPPAEKIHDTETFWASADVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWFVEKTATAAASRAKADEVASSRAAVAVAVTPLDIPRSSPXXXXXXXXXXXXNRRCRRVLWFNASHERDVVPLLDMLASARRRESCDGSSGNAXXXXXXXXXXXADGDTPLFDEAWFMEVIPGRPSRFKLPTAQEILAPHGISPAVAAAAATSXXXXXXXXXXXGPCSDDPSGEEPAGGAAPGCGEEKADGAGAXSGAGAWQRTLEQVWTSLQTRRGGTLSATLTGTAPFFFEKLSLICANSQHDGGSEAASVVGDPGVASRTAVSAVDADHHGSGGRGDASVAATGCGDRGGRVDSEIVEVLCTGSLYAVAAALEAFGAEVE 731          

BLAST of mRNA_E-siliculosus-1a_F_contig1011.128.1 vs. uniprot
Match: A0A6H5L5N3_9PHAE (Tetrahydrofolate synthase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L5N3_9PHAE)

HSP 1 Score: 840 bits (2171), Expect = 4.430e-302
Identity = 455/536 (84.89%), Postives = 464/536 (86.57%), Query Frame = 0

Query:    1 MVESVLRHHGFRTAGLYTSPHLLEVTERVRCNGKPLTKEAFARYFFQVWDRLQDSAARTNT---------ATGTTGIPAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPPPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASQQCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCVVWIEAHRQQXXXXXAGDQAPPPEKIRNTEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWFVEKTATAAASRAKADEVVSSRAAVAVAVTPREIPRSSPXXXXXXXXXXXQKRRCRRVLWFNASHERDVVPLLHMLASARRREPCNGSSGNATNTTNKDDHNDADGDTPLFDDAWFMEVNPGRPSRFKLPTAQEILAPHGMSPAVAAAAATPAGIAGASSVSSGPCXXXXXXXXXXXXXXXXXGEEKADSAGAESGAGAWQRTLEQ 527
            MVESVLRHHGFRT GLYTSPHLLEVTERVRC+GKPLTKEAFARYFFQVWDRLQD AARTNT         ATGTTGI AAASEGPSQA+QGP APEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVP PVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREAS +CAAAAGC ELEVVKPLP RDPCGRAYEIGLAGDHQRVNAALALRLC VWIEAHRQQXXXXXAGDQAPPPEKIR TEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTI+LDGAHTEQSMRACLGWFVEK           DEV SSRAAVAVAVTPR+IPRSSPXXXXX      QKRRCRRVLWFNASHERDVVPLL MLASARRRE  NGS           D +DADGDTPLF++AWFMEVN GRPSRFKLPTAQEILA HGM PAVAAAAATP G+AGAS V SGP     XXXXXX       GEEKAD AGA SGAGAWQRTLEQ
Sbjct:    1 MVESVLRHHGFRT-GLYTSPHLLEVTERVRCDGKPLTKEAFARYFFQVWDRLQDPAARTNTDDATAAAVAATGTTGITAAASEGPSQAKQGPTAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASPKCAAAAGCSELEVVKPLPARDPCGRAYEIGLAGDHQRVNAALALRLCDVWIEAHRQQXXXXXAGDQAPPPEKIRETEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIHLDGAHTEQSMRACLGWFVEK----------GDEVASSRAAVAVAVTPRDIPRSSPXXXXXQLELRRQKRRCRRVLWFNASHERDVVPLLDMLASARRREARNGSXXXXXXXXXXADDDDADGDTPLFEEAWFMEVNTGRPSRFKLPTAQEILASHGMFPAVAAAAATPPGMAGASGVGSGPSSDDPXXXXXXGGAAPGCGEEKADGAGAXSGAGAWQRTLEQ 525          

BLAST of mRNA_E-siliculosus-1a_F_contig1011.128.1 vs. uniprot
Match: UPI0005CC571D (folylpolyglutamate synthase, mitochondrial isoform X2 n=2 Tax=Oryzias latipes TaxID=8090 RepID=UPI0005CC571D)

HSP 1 Score: 233 bits (594), Expect = 8.120e-65
Identity = 133/322 (41.30%), Postives = 182/322 (56.52%), Query Frame = 0

Query:    3 ESVLRHHGFRTAGLYTSPHLLEVTERVRCNGKPLTKEAFARYFFQVWDRLQDSAARTNTATGTTGIPAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPPPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASQ--QCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCVVWIEAHRQQXXXXXAGDQAPPPEKIRNTEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWF 322
            E +LR +G RT G Y+SPHL++V ER+R NG+P+ K+ F +YF+Q++ RL++S                              P   GT    MP YF  LT++    F+QE VDV V+EVG+GG +D TN++  P  CGVA L +DHTQ+LGDT++KIAW+KGGIFK G  A  V QP G M+VLR+ ++  QC        L V   L      G A  +GLAG HQR NA+LAL+L   W++          A +  P   +++   AF  S+E+  GL+   WPGR Q  +      VT +LDGAHT +SM+AC+ WF
Sbjct:  118 EKILRSYGLRT-GFYSSPHLVQVRERIRINGQPIGKDIFTKYFWQIYGRLEESK-----------------------------PASGGT----MPAYFRFLTILAFHVFLQEKVDVAVIEVGIGGAYDCTNIIRKPWVCGVASLGIDHTQILGDTIEKIAWQKGGIFKPGVPAFTVSQPEGAMAVLRDRAKEIQC-------PLWVCPNLDEYQTDGTALHLGLAGKHQRWNASLALQLSNSWLKRRCSPDQSLSAIN--PENTRVQPATAFRPSSEMMMGLANTEWPGRNQTLKNG---EVTYFLDGAHTVRSMQACVDWF 393          

BLAST of mRNA_E-siliculosus-1a_F_contig1011.128.1 vs. uniprot
Match: A0A0S7K0M8_9TELE (Tetrahydrofolate synthase (Fragment) n=2 Tax=Poeciliopsis prolifica TaxID=188132 RepID=A0A0S7K0M8_9TELE)

HSP 1 Score: 228 bits (581), Expect = 9.640e-65
Identity = 130/327 (39.76%), Postives = 183/327 (55.96%), Query Frame = 0

Query:    3 ESVLRHHGFRTAGLYTSPHLLEVTERVRCNGKPLTKEAFARYFFQVWDRLQDSAARTNTATGTTGIPAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPPPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASQQCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCVVWIEAHRQQXXXXXAGDQAPPPEK--IRNTEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWFVEKTA 327
            E +LR +GFRT G Y+SPHL++V ER+R +G+P++ E F +YF+QV+ RL     +T  A G T                             MP YF  LT++    F+QE VD+ ++EVG+GG +D TNVV  P  CGV+ L +DHTQ+LGDT++KIAW+K GIFK G  A  V QP+  M+VLR+ +++         L V   L        A ++GLAG HQR NA+LAL+LC  W+    Q+     +    P P+   +   ++F   A + +GL+   W GR Q  R  A   +T +LDGAHT +SM+AC+ WF E  A
Sbjct:   37 EQILRTYGFRT-GFYSSPHLVQVRERIRIDGRPISNELFTKYFWQVYGRLD----KTKEAHGGT-----------------------------MPAYFRFLTILAFHVFLQEKVDLAIIEVGIGGAYDCTNVVRRPWVCGVSSLGIDHTQILGDTIEKIAWQKAGIFKPGVPAFTVRQPQEAMAVLRDRAREIRCP-----LRVCPDLDRYQTESGALQLGLAGQHQRSNASLALQLCHTWL----QRRCGPDSSPALPDPDSSCLSQADSFRPGAAMVKGLADTHWAGRNQTLRRGA---LTYFLDGAHTHRSMQACVQWFQEAAA 317          

BLAST of mRNA_E-siliculosus-1a_F_contig1011.128.1 vs. uniprot
Match: H2MVP5_ORYLA (Folylpolyglutamate synthase n=5 Tax=Oryzias latipes TaxID=8090 RepID=H2MVP5_ORYLA)

HSP 1 Score: 233 bits (593), Expect = 1.150e-64
Identity = 133/322 (41.30%), Postives = 181/322 (56.21%), Query Frame = 0

Query:    3 ESVLRHHGFRTAGLYTSPHLLEVTERVRCNGKPLTKEAFARYFFQVWDRLQDSAARTNTATGTTGIPAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPPPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASQ--QCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCVVWIEAHRQQXXXXXAGDQAPPPEKIRNTEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWF 322
            E +LR +G RT G Y+SPHL++V ER+R NG+P+ K+ F +YF+Q++ RL++S                              P   GT    MP YF  LT++    F+QE VDV V+EVG+GG +D TN++  P  CGVA L +DHTQ+LGDT++KIAW+KGGIFK G  A  V QP G M+VLR+ ++  QC        L V   L      G A  +GLAG HQR NA+LAL+L   W++  R            P   +++   AF  S+E+  GL+   WPGR Q  +      VT +LDGAHT +SM+AC+ WF
Sbjct:  118 EKILRSYGLRT-GFYSSPHLVQVRERIRINGQPIGKDIFTKYFWQIYGRLEESK-----------------------------PASGGT----MPAYFRFLTILAFHVFLQEKVDVAVIEVGIGGAYDCTNIIRKPWVCGVASLGIDHTQILGDTIEKIAWQKGGIFKPGVPAFTVSQPEGAMAVLRDRAKEIQC-------PLWVCPNLDEYQTDGTALHLGLAGKHQRWNASLALQLSNSWLK-RRCSPVDQSLSAINPENTRVQPATAFRPSSEMMMGLANTEWPGRNQTLKNG---EVTYFLDGAHTVRSMQACVDWF 394          

BLAST of mRNA_E-siliculosus-1a_F_contig1011.128.1 vs. uniprot
Match: UPI000719C602 (folylpolyglutamate synthase, mitochondrial-like n=1 Tax=Priapulus caudatus TaxID=37621 RepID=UPI000719C602)

HSP 1 Score: 230 bits (587), Expect = 1.720e-64
Identity = 131/326 (40.18%), Postives = 178/326 (54.60%), Query Frame = 0

Query:    3 ESVLRHHGFRTAGLYTSPHLLEVTERVRCNGKPLTKEAFARYFFQVWDRLQDSAARTNTATGTTGIPAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPPPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASQQCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCVVWIEA-HRQQXXXXXAGDQAPPP-----EKIRNTEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWF 322
            ES+LR +G++T GL+TSPHL+E  ER+R NG P+ K+ FA YFF+V++RL ++  + +   G                               MP YF  LTL+ L+ FV++ VDV +LE G+GG +D TN+V  PV CGV+ L +DH  +LGDT++KIAW K GIFK+   A+ V QP G M VL E +++  A      L +   + T D      ++GLAGD Q  NA+LAL+LC VW+EA H+        G Q         +K++    F  + E   GL  C WPGR Q  R      VT +LDGAHT  SM  C  WF
Sbjct:   78 ESILRQYGYKT-GLFTSPHLVETRERIRINGLPIEKKLFASYFFEVYNRLINTKNQHDPEVG-------------------------------MPAYFRFLTLMSLYVFVEQKVDVAILECGIGGTYDCTNIVRKPVVCGVSSLGIDHVSLLGDTVEKIAWHKAGIFKDDVAAVTVPQPAGPMQVLSERARELGA-----RLTLAPDIHTYDRERAPPQLGLAGDMQLWNASLALQLCKVWLEARHQGCGDRRGVGGQRQNQVNGTRDKVQTAPTFPITTEFSEGLKNCFWPGRAQTLRRSR---VTYFLDGAHTASSMETCARWF 363          

BLAST of mRNA_E-siliculosus-1a_F_contig1011.128.1 vs. uniprot
Match: UPI0014028A9D (folylpolyglutamate synthase, mitochondrial n=1 Tax=Amblyraja radiata TaxID=386614 RepID=UPI0014028A9D)

HSP 1 Score: 233 bits (593), Expect = 2.210e-64
Identity = 128/334 (38.32%), Postives = 192/334 (57.49%), Query Frame = 0

Query:    3 ESVLRHHGFRTAGLYTSPHLLEVTERVRCNGKPLTKEAFARYFFQVWDRLQDSAARTNTATGTTGIPAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPPPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASQQCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCVVWIEAHRQQXXXXXAGDQAPPPEKIRNT----EAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWFVEKTATAAAS 332
            E++LR++G +T G Y+SPHL++V ER+R NGKP++K+ F +YF+QV++RL+++  R                             + GT    MP YF  LT++    F+ E +DV +LEVG+GG +D TN++  PV CGV+ L +DH  +LGDT++KIAW+KGGIFK G  A +V QP G M+VL E +++     GC  L++   L           +GLAG+HQ++NA+LAL+LC  W++ H         G Q    + ++ +     AF  S  +  GL    W GR Q+ +     SVT Y+DGAHT  S++AC+ WF E+++  A S
Sbjct:  126 ENILRNYGLKT-GFYSSPHLVQVRERIRINGKPISKDLFTKYFWQVYNRLEETKDR-----------------------------DSGT----MPAYFRFLTILAFHIFLHEKIDVAILEVGIGGAYDCTNIIRHPVVCGVSSLGIDHVSILGDTMEKIAWQKGGIFKPGVPAFSVPQPSGPMAVLVERAKEI----GCS-LQLCSDLEDFQSEREKLTLGLAGNHQKLNASLALQLCREWLQYHHHPDFPQLQGKQDHAEQHLKQSLPVSSAFCPSTAMVEGLRNTEWLGRSQILKRG---SVTYYIDGAHTIGSIKACVTWFREQSSKEAKS 417          

BLAST of mRNA_E-siliculosus-1a_F_contig1011.128.1 vs. uniprot
Match: F1Q7N0_DANRE (Folylpolyglutamate synthase n=16 Tax=Cyprinoidei TaxID=30727 RepID=F1Q7N0_DANRE)

HSP 1 Score: 231 bits (589), Expect = 3.960e-64
Identity = 133/324 (41.05%), Postives = 186/324 (57.41%), Query Frame = 0

Query:    3 ESVLRHHGFRTAGLYTSPHLLEVTERVRCNGKPLTKEAFARYFFQVWDRLQDSAARTNTATGTTGIPAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPPPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASQQCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCVVWIEAHRQQXXXXXAGDQAPPPE--KIRNTEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWFVE 324
            E +LR++GFRT G Y+SPHL++V ER+R NG+P+ KE F +YF+QV+ RL++    T  A G +                             MP YF  LT++    F+QE VD+ V+EVG+GG +D TN++  P  CG++ L +DHT +LGDT++KIAW+KGGIFK G  A  V+QP G M VL+E +++     GC  L V   L   +  G +  +GLAG HQR NA+LAL+L   W++ H          D A P E   + +  AF  S  + +GL+   WPGR Q  +      VT Y+DGAHT +SM+AC+ WF E
Sbjct:  117 EKILRNYGFRT-GFYSSPHLVQVRERIRINGQPIGKELFTKYFWQVYGRLEE----TKDAHGGS-----------------------------MPAYFRFLTILAFHVFLQEKVDLAVIEVGIGGAYDCTNIIRRPWVCGISSLGIDHTSILGDTIEKIAWQKGGIFKPGVPAFTVKQPDGPMKVLQERAEEV----GCS-LSVCPDLDEYESEG-SLSLGLAGQHQRSNASLALQLSHTWLQRH-------ILTDPASPVEFSGVSSAPAFQPSPAMIKGLAETEWPGRNQTLK---HGPVTYYMDGAHTTRSMQACVRWFNE 390          

BLAST of mRNA_E-siliculosus-1a_F_contig1011.128.1 vs. uniprot
Match: A0A8C7ZZ20_9TELE (Folylpolyglutamate synthase n=7 Tax=Oryzias sinensis TaxID=183150 RepID=A0A8C7ZZ20_9TELE)

HSP 1 Score: 230 bits (587), Expect = 1.100e-63
Identity = 132/322 (40.99%), Postives = 186/322 (57.76%), Query Frame = 0

Query:    3 ESVLRHHGFRTAGLYTSPHLLEVTERVRCNGKPLTKEAFARYFFQVWDRLQDSAARTNTATGTTGIPAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPPPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASQ--QCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCVVWIEAHRQQXXXXXAGDQAPPPEKIRNTEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWF 322
            E +LR +GFRT G Y+SPHL++V ER+R NG+P+ K+ F++YF+Q++ RL++S  + + + GT                              MP YF  LT++    F+QE VDV V+EVG+GG +D TN++  P  CGVA L +DHTQ+LGDT++KIAW+KGGIFK G  A  V QP G M+VLR+ ++  QC        L +   L      G A  +GLAG HQR NA+LAL+L   W++           G  +   + + +T +F  S+E+  GL+   WPGR Q  R      VT +LDGAHT +SM+AC+ WF
Sbjct:  136 EKILRSYGFRT-GFYSSPHLVQVRERIRINGQPIGKDIFSKYFWQIYGRLEES--KVDASGGT------------------------------MPAYFRFLTILAFHVFLQEKVDVAVIEVGIGGAYDCTNIIRKPWVCGVASLGIDHTQILGDTIEKIAWQKGGIFKPGVPAFTVSQPEGAMAVLRDRAKEIQC-------PLWLCPNLDDYQTDGTALHLGLAGKHQRWNASLALQLSNSWLKRR------CSPGKSSRMGQGLLST-SFRPSSEMMMGLANTEWPGRNQTLRNG---EVTYFLDGAHTVRSMQACVDWF 407          

BLAST of mRNA_E-siliculosus-1a_F_contig1011.128.1 vs. uniprot
Match: A0A2I4CSR9_9TELE (Folylpolyglutamate synthase n=1 Tax=Austrofundulus limnaeus TaxID=52670 RepID=A0A2I4CSR9_9TELE)

HSP 1 Score: 230 bits (586), Expect = 1.160e-63
Identity = 147/395 (37.22%), Postives = 199/395 (50.38%), Query Frame = 0

Query:    3 ESVLRHHGFRTAGLYTSPHLLEVTERVRCNGKPLTKEAFARYFFQVWDRLQDSAARTNTATGTTGIPAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPPPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASQQCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCVVWIEAHRQQXXXXXAGDQAPPPEKIRNTEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWFVEKTATAAASRAKADEVVSSRAAVAVAVTPREIPRSSPXXXXXXXXXXXQKRRCRRVLWFNASHERDVVPLLHML 397
            E +LR +GFRT G Y+SPHL++V ER+R NG+P++K+ F +YF+QV+ RL +    T  A G                               MP YF  LT++    F+QE VDV V+EVG+GG +D TN++  P  CGV+ L +DHTQ+LGDT++KIAW+KGGIFK G  A  V QP   MSVLRE S++         L V   L           +GLAG HQR NA+LAL+L   W++  R+      +        ++   ++F     + +GL+   WPGR Q  R  A   VT +LDGAHT +SM+AC+ WF E                        AV P+E   S P                 RVL FNA+ ERD   +L +L
Sbjct:  119 EQILRSYGFRT-GFYSSPHLVQVRERIRINGQPISKDLFTKYFWQVYGRLDE----TKEAAGGA-----------------------------MPAYFRFLTILAFHIFLQEKVDVAVIEVGIGGAYDCTNIIRRPWVCGVSSLGIDHTQILGDTIEKIAWQKGGIFKPGVPAFTVRQPEEAMSVLRERSREIRCP-----LWVCPELDQYQTDSXPLSLGLAGQHQRSNASLALQLSHTWLQ--RRCGPDQSSSVSELQDFRVSQADSFRPGPFMLKGLADTEWPGRTQTLRHGA---VTYFLDGAHTMRSMQACVQWFRE------------------------AVAPQERTSSGPVA---------------RVLLFNATGERDSSAMLKLL 430