mRNA_E-siliculosus-1a_F_contig1083.580.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig1083.580.1
Unique NamemRNA_E-siliculosus-1a_F_contig1083.580.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig1083.580.1 vs. uniprot
Match: D8LRB7_ECTSI (Similar to calmodulin 2 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LRB7_ECTSI)

HSP 1 Score: 175 bits (443), Expect = 1.960e-52
Identity = 115/132 (87.12%), Postives = 116/132 (87.88%), Query Frame = 1 
Query:  130 KLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKRGTLDFNEFKQLMAARLTYKNTRAEFXXXXXXXXXXXXXXXXXXXXXXXXVATGFGLTNQELKEIFGLXXXXXXXXXXMVEFLDYMMDA 525
            KLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADK GTLDFNEF+ LM ARLTYKNTRAEFXXXXXXXXXXXXXXXXXXXXXXX VATGFGLTNQELKEIFG           M EFLDYMMDA
Sbjct:   72 KLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKTGTLDFNEFRLLMTARLTYKNTRAEFXXXXXXXXXXXXXXXXXXXXXXXMVATGFGLTNQELKEIFGFFDSDGSGKVDMAEFLDYMMDA 203
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BLAST of mRNA_E-siliculosus-1a_F_contig1083.580.1 vs. uniprot
Match: A0A6H5JR81_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JR81_9PHAE)

HSP 1 Score: 156 bits (395), Expect = 3.290e-45
Identity = 110/136 (80.88%), Postives = 112/136 (82.35%), Query Frame = 1 
Query:  127 FKLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKRGTLDFNEFKQLMAARLTYK---NTRAEFXXXXXXXXXXXXXXXXXXXXXXXXVATGFGLTNQELKEIFGLXXXXXXXXXXMVEFLDYMMDA 525
             KLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADK GTLDFNEFK LM ARLTYK   N     XXXXXXXXXXXXXXXXXXXXXXXXVA+GFGLTN+ELKEIFG           M EFLDYMMDA
Sbjct:   65 IKLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKTGTLDFNEFKLLMTARLTYKGDQNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVASGFGLTNRELKEIFGFFDRDGSGKVDMAEFLDYMMDA 200
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BLAST of mRNA_E-siliculosus-1a_F_contig1083.580.1 vs. uniprot
Match: A0A564YBB7_HYMDI (Uncharacterized protein (Fragment) n=1 Tax=Hymenolepis diminuta TaxID=6216 RepID=A0A564YBB7_HYMDI)

HSP 1 Score: 68.6 bits (166), Expect = 3.490e-12
Identity = 37/74 (50.00%), Postives = 46/74 (62.16%), Query Frame = 1 
Query:   88 ELTAEEQDEIVQIFKLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKRGTLDFNEFKQLMAARL 309
            +LTAE+     + F   D NGDG +T  EL   + SLGQ PTE+E   I R  DADK GT+DFNEF  LM+ R+
Sbjct:    3 KLTAEQIAAFKESFAFFDKNGDGVITTGELGRVMKSLGQEPTEKELQEIIRTVDADKSGTIDFNEFLGLMSKRI 76
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BLAST of mRNA_E-siliculosus-1a_F_contig1083.580.1 vs. uniprot
Match: A0A6G1DCE5_9ORYZ (Uncharacterized protein n=1 Tax=Oryza meyeriana var. granulata TaxID=110450 RepID=A0A6G1DCE5_9ORYZ)

HSP 1 Score: 68.9 bits (167), Expect = 1.190e-11
Identity = 36/70 (51.43%), Postives = 47/70 (67.14%), Query Frame = 1 
Query:   91 LTAEEQDEIVQIFKLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKRGTLDFNEFKQLMA 300
            LTAE+ +++ ++F L D N DG VT  EL + L SLGQN TE E  ++ RE DAD  G +DF+EF  LMA
Sbjct:    5 LTAEQIEQLKEVFALFDKNNDGIVTSQELGEVLSSLGQNHTEAELQNMIREVDADGNGVIDFHEFLNLMA 74
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BLAST of mRNA_E-siliculosus-1a_F_contig1083.580.1 vs. uniprot
Match: T1EEY3_HELRO (Calmodulin n=1 Tax=Helobdella robusta TaxID=6412 RepID=T1EEY3_HELRO)

HSP 1 Score: 68.2 bits (165), Expect = 2.450e-11
Identity = 34/76 (44.74%), Postives = 48/76 (63.16%), Query Frame = 1 
Query:   82 ERELTAEEQDEIVQIFKLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKRGTLDFNEFKQLMAARL 309
            + +LT EE  E  + F L D NGDG+++ +EL   + SLGQNPTE E   +  E D D  GT+DF+EF  +MA ++
Sbjct:    4 DHKLTDEEVAEFREAFSLFDKNGDGTISSTELGTIMRSLGQNPTESELQDMINEVDVDGNGTIDFDEFLDMMAKKM 79
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BLAST of mRNA_E-siliculosus-1a_F_contig1083.580.1 vs. uniprot
Match: A0A8H6SRD0_9AGAR (Calmodulin-like protein n=1 Tax=Mycena indigotica TaxID=2126181 RepID=A0A8H6SRD0_9AGAR)

HSP 1 Score: 67.8 bits (164), Expect = 4.670e-11
Identity = 36/79 (45.57%), Postives = 50/79 (63.29%), Query Frame = 1 
Query:   88 ELTAEEQDEIVQIFKLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKRGTLDFNEFKQLMAARLTYKNT 324
            +L+ E+  E  + F L D +GDG++T  EL   + SLGQNPTE E   +  E DAD  GT+DFNEF  +MA +  +K+T
Sbjct:    4 QLSPEQISEFKEAFSLFDKDGDGTITTLELGTVMRSLGQNPTESELQDMINEVDADGNGTIDFNEFLDMMAKK--FKDT 80
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BLAST of mRNA_E-siliculosus-1a_F_contig1083.580.1 vs. uniprot
Match: A0A1S3IRI9_LINUN (Calmodulin n=4 Tax=Lingula unguis TaxID=7574 RepID=A0A1S3IRI9_LINUN)

HSP 1 Score: 67.8 bits (164), Expect = 5.250e-11
Identity = 35/73 (47.95%), Postives = 46/73 (63.01%), Query Frame = 1 
Query:   91 LTAEEQDEIVQIFKLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKRGTLDFNEFKQLMAARL 309
            LT E+  E  + F L D +GDG++T SEL   + SLGQNPTE E   +  E D D  GT+DF EF Q+MA ++
Sbjct:   28 LTEEQIAEFKEAFSLFDKDGDGTITTSELGTVMRSLGQNPTEAELQDMINEVDEDGNGTIDFEEFLQMMAKKM 100
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BLAST of mRNA_E-siliculosus-1a_F_contig1083.580.1 vs. uniprot
Match: K1QRR1_CRAGI (Calmodulin n=2 Tax=Crassostrea TaxID=6564 RepID=K1QRR1_CRAGI)

HSP 1 Score: 67.4 bits (163), Expect = 5.450e-11
Identity = 36/82 (43.90%), Postives = 51/82 (62.20%), Query Frame = 1 
Query:   88 ELTAEEQDEIVQIFKLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKRGTLDFNEFKQLMAARLTYKNTRAE 333
            +L+ E+  E  + F L D +GDGS+T  EL   + SLGQNPTE E   +  E D D+ GT+DF+EF Q+MA ++   +T  E
Sbjct:    8 KLSDEQVAEFREAFNLFDKDGDGSITTMELGTVMRSLGQNPTEGELQDMINEVDYDESGTIDFDEFLQMMARKMRDTDTTEE 89
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BLAST of mRNA_E-siliculosus-1a_F_contig1083.580.1 vs. uniprot
Match: UPI00201F3592 (calmodulin-A-like n=2 Tax=Haliotis TaxID=6452 RepID=UPI00201F3592)

HSP 1 Score: 67.0 bits (162), Expect = 7.940e-11
Identity = 34/73 (46.58%), Postives = 47/73 (64.38%), Query Frame = 1 
Query:   91 LTAEEQDEIVQIFKLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKRGTLDFNEFKQLMAARL 309
            L+ E+  E  + F L D +GDG+++  EL   + SLGQNPTEEE   +  E DAD  GT+DF EF Q+MA ++
Sbjct:    8 LSEEQIAEFKEAFSLFDKDGDGTISHKELGVVMRSLGQNPTEEELQEMINEVDADGNGTIDFEEFLQMMAKKM 80
[Close]
BLAST of mRNA_E-siliculosus-1a_F_contig1083.580.1 vs. uniprot
Match: A0A2D0HB79_9NOSO (Thiol oxidase n=17 Tax=Nostocales TaxID=1161 RepID=A0A2D0HB79_9NOSO)

HSP 1 Score: 70.1 bits (170), Expect = 7.950e-11
Identity = 42/121 (34.71%), Postives = 65/121 (53.72%), Query Frame = 1 
Query:   91 LTAEEQDEIVQIFKLCDVNGDGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKRGTLDFNEFKQLMAARLTYKNTRAEFXXXXXXXXXXXXXXXXXXXXXXXXVATGFGLTNQELKEI 453
            +  +E +++ Q FK+ DV+G+G+++  EL + + SLGQNPTE     + +E D D  GT+DF EFK LM A++  + +R +                         V T FGLT+ ELKE+
Sbjct:    2 IDEQEIEKLWQAFKVLDVDGNGAISTEELGEVMRSLGQNPTEAGLRDLIKEIDVDLSGTIDFEEFKTLMIAKVGDRESRLKLAFSAFDEDGSGEITAVELRT----VMTQFGLTDAELKEM 118
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig1083.580.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LRB7_ECTSI1.960e-5287.12Similar to calmodulin 2 n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JR81_9PHAE3.290e-4580.88Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A564YBB7_HYMDI3.490e-1250.00Uncharacterized protein (Fragment) n=1 Tax=Hymenol... [more]
A0A6G1DCE5_9ORYZ1.190e-1151.43Uncharacterized protein n=1 Tax=Oryza meyeriana va... [more]
T1EEY3_HELRO2.450e-1144.74Calmodulin n=1 Tax=Helobdella robusta TaxID=6412 R... [more]
A0A8H6SRD0_9AGAR4.670e-1145.57Calmodulin-like protein n=1 Tax=Mycena indigotica ... [more]
A0A1S3IRI9_LINUN5.250e-1147.95Calmodulin n=4 Tax=Lingula unguis TaxID=7574 RepID... [more]
K1QRR1_CRAGI5.450e-1143.90Calmodulin n=2 Tax=Crassostrea TaxID=6564 RepID=K1... [more]
UPI00201F35927.940e-1146.58calmodulin-A-like n=2 Tax=Haliotis TaxID=6452 RepI... [more]
A0A2D0HB79_9NOSO7.950e-1134.71Thiol oxidase n=17 Tax=Nostocales TaxID=1161 RepID... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig1083contigE-siliculosus-1a_F_contig1083:23286..25014 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start1
Stop1
Cds size528
Model size528
Exons3
Ec32 orthologEc-06_003120.1
Ec32 ortholog descriptionEF-hand domain pair
Hectar predicted targeting categoryother localisation
BRITEko00000,ko00001,ko01009,ko03036,ko04131,ko04147
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
COG Functional cat.DTZ
EggNOG OGsCOG5126@1,KOG0027@2759
EggNOG free text desc.Ca2 -binding protein (EF-Hand superfamily
GOsGO:0000142,GO:0000910,GO:0000916,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005826,GO:0005856,GO:0005933,GO:0005935,GO:0005938,GO:0006996,GO:0007010,GO:0007049,GO:0008092,GO:0008150,GO:0009987,GO:0015629,GO:0016043,GO:0016459,GO:0016460,GO:0017022,GO:0022402,GO:0030029,GO:0030036,GO:0030427,GO:0030863,GO:0030864,GO:0030865,GO:0030866,GO:0031032,GO:0032153,GO:0032155,GO:0032506,GO:0032991,GO:0036213,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0044422,GO:0044424,GO:0044430,GO:0044444,GO:0044446,GO:0044448,GO:0044464,GO:0044837,GO:0045159,GO:0051301,GO:0061640,GO:0070938,GO:0071840,GO:0071944,GO:0099568,GO:0110085
KEGG Pathwayko04014,ko04015,ko04016,ko04020,ko04022,ko04024,ko04070,ko04114,ko04218,ko04261,ko04270,ko04371,ko04626,ko04713,ko04720,ko04722,ko04728,ko04740,ko04744,ko04745,ko04750,ko04910,ko04912,ko04915,ko04916,ko04921,ko04922,ko04924,ko04925,ko04970,ko04971,ko05010,ko05031,ko05034,ko05133,ko05152,ko05167,ko05200,ko05214,ko05418,map04014,map04015,map04016,map04020,map04022,map04024,map04070,map04114,map04218,map04261,map04270,map04371,map04626,map04713,map04720,map04722,map04728,map04740,map04744,map04745,map04750,map04910,map04912,map04915,map04916,map04921,map04922,map04924,map04925,map04970,map04971,map05010,map05031,map05034,map05133,map05152,map05167,map05200,map05214,map05418
KEGG koko:K02183
Preferred nameMLC2
Seed eggNOG ortholog2880.D8LRB7
Seed ortholog evalue4e-42
Seed ortholog score177.9
Taxonomic scopeEukaryota
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig1083.580.1prot_E-siliculosus-1a_F_contig1083.580.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig1083 23286..25014 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927584.9741955-CDS-E-siliculosus-1a_F_contig1083:23285..234951622927584.9741955-CDS-E-siliculosus-1a_F_contig1083:23285..23495Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1083 23286..23495 -
1696318598.2707093-CDS-E-siliculosus-1a_F_contig1083:23285..234951696318598.2707093-CDS-E-siliculosus-1a_F_contig1083:23285..23495Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1083 23286..23495 -
1622927584.9899762-CDS-E-siliculosus-1a_F_contig1083:24374..245601622927584.9899762-CDS-E-siliculosus-1a_F_contig1083:24374..24560Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1083 24375..24560 -
1696318598.282762-CDS-E-siliculosus-1a_F_contig1083:24374..245601696318598.282762-CDS-E-siliculosus-1a_F_contig1083:24374..24560Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1083 24375..24560 -
1622927585.002805-CDS-E-siliculosus-1a_F_contig1083:24882..250141622927585.002805-CDS-E-siliculosus-1a_F_contig1083:24882..25014Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1083 24883..25014 -
1696318598.2919195-CDS-E-siliculosus-1a_F_contig1083:24882..250141696318598.2919195-CDS-E-siliculosus-1a_F_contig1083:24882..25014Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1083 24883..25014 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig1083.580.1

>prot_E-siliculosus-1a_F_contig1083.580.1 ID=prot_E-siliculosus-1a_F_contig1083.580.1|Name=mRNA_E-siliculosus-1a_F_contig1083.580.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=176bp
MSRSGAAASVIGRVVGHVSPSRSRVTGERELTAEEQDEIVQIFKLCDVNG
DGSVTKSELKDALWSLGQNPTEEETDHIFREYDADKRGTLDFNEFKQLMA
ARLTYKNTRAEFEDAFRAIDHNKDGKVDITELNRLMVATGFGLTNQELKE
IFGLFDRDGSGKVDMVEFLDYMMDA*
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mRNA from alignment at E-siliculosus-1a_F_contig1083:23286..25014-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig1083.580.1 ID=mRNA_E-siliculosus-1a_F_contig1083.580.1|Name=mRNA_E-siliculosus-1a_F_contig1083.580.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=1729bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1083:23286..25014- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGTCCCGCTCGGGTGCTGCTGCATCAGTTATCGGCAGAGTCGTCGGTCA
TGTGTCCCCGTCTAGGTCGCGCGTTACAGGCGAAAGAGAGCTCACGGCAG
AAGAGCAAGATGAAATAGTGCAAATATTCAAGGTAATATCTTCTGCTGGC
ATTTTTCGGGACCTCGGTTACGAGGAAAATGTAGCGCAAGGAAATCGCTC
ATCATGCGACAGTCAAGTTTTAGGAGCAAACGCCTGGAGCACAGATTGTG
CGGGTTCAATACAAGTGGAGAGTGTACGGTACTATATAAGGGGAAATTGT
ACATTCTGCACAGGAAACACTCACAACAGCGATCTAGGAGACACAGAAAA
CAATCTTGCAACCCAACGGACGACTTCGCGGCGGACCCTCGCTTATTTTG
TGTGTGAAAAATTGTGGCTTGTACAAATGCCGGTCTTCTCTTTCCCGAAC
AAAGCTGTGTGACGTGAACGGAGACGGGTCCGTTACGAAGTCCGAGCTGA
AAGATGCGCTTTGGTCACTGGGGCAGAATCCGACCGAGGAAGAGACCGAC
CACATATTTCGCGAGTACGATGCAGACAAGAGAGGCACCCTGGATTTCAA
CGAGTTCAAGCAACTAATGGCGGCAAGGCTCACGTACAAGGTGGGTCTCT
TTCTCCCTCCTTTGCAGCTGAAACATCGTCCGTGTTGTGTATTCAAGGGC
TTGGTTGGAAGAAATATCGAAATTTGAAAGATATTCGTAGTACAATTGTA
CAGTATTGCACGTGAGCGTAGCATCGAATTATTATTGCGAAACATGGAGC
ACGAAGTACTGGTATTATTACGAAGGGTAAGGGTCCACGGCAGCCACACA
AAGAAGAACGATACATGTGGTGCATACGACGGTACGCTGGTGAGAGCACC
CCGAGCAAACGAATGCGTGATATAAGGACCTTCCTCTTTATACTCAGGAC
AAAAGCGCAAATCGCCCAGGTCTAAGTTCCCGGTGCGGTTTGTGAAATAT
TCATTACATAGACTATGTGGTCGATCTCCATGATGAGGTCATGGCGTGAG
GATTCACGGAGATATGTGCTTCAAAACGATCACAGCAACCATCAGAATAT
CGTCCTTGATCATACTTGCGAGGGAAGAAAACAATATTCTGTAGGTCATT
CCGGGCATTGTTGTTGGGCCTTGAATCTCTGGGAGAAGGGTCACCCTTCG
AAGAGAAGAGCTGGAATGCCGTATTACACCTTTGCTCTATCCTACATCGA
ACGGCAAGCAAGCCCGGAGGCTTGACATTCTGCTGATGAGGCTTCAGCTT
TACATTCTTCTCAAGGCTGATTAGCCGCTGTCAGGCAAGTGCGTCTTTCG
TGCAAACAGGGGGACCAGTGCGAAACGTTCGTGCAGGCCCGTATCCTCAA
CCACCACTACTTTGCTGCGGGCTGGTCGGCGGCACAATACACGTGCACTT
CGTCGTTCCCACTACTTTCCTCTCTCTCCCGCCGCTTCCCCATGGGTACC
CAATACTTGCAAACAACAGAACACACGGGCGGAGTTTGAGGATGCTTTCC
GAGCGATCGATCACAACAAGGATGGAAAGGTCGACATAACAGAGCTCAAT
AGGCTCATGGTTGCGACAGGGTTTGGACTGACCAACCAAGAGTTGAAAGA
AATTTTTGGCCTTTTCGACCGTGACGGCAGCGGGAAGGTAGACATGGTAG
AATTTTTGGACTATATGATGGACGCGTAA
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig1083:23286..25014-

>mRNA_E-siliculosus-1a_F_contig1083.580.1 ID=mRNA_E-siliculosus-1a_F_contig1083.580.1|Name=mRNA_E-siliculosus-1a_F_contig1083.580.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=1056bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1083:23286..25014- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGTCCCGCTCGGGTGCTGCTGCATCAGTTATCGGCAGAGTCGTCGGTCA
TGTGTCCCCGTCTAGGTCGCGCGTTACAGGCGAAAGAGAGCTCACGGCAG
AAGAGCAAGATGAAATAGTGCAAATATTCAAGATGTCCCGCTCGGGTGCT
GCTGCATCAGTTATCGGCAGAGTCGTCGGTCATGTGTCCCCGTCTAGGTC
GCGCGTTACAGGCGAAAGAGAGCTCACGGCAGAAGAGCAAGATGAAATAG
TGCAAATATTCAAGCTGTGTGACGTGAACGGAGACGGGTCCGTTACGAAG
TCCGAGCTGAAAGATGCGCTTTGGTCACTGGGGCAGAATCCGACCGAGGA
AGAGACCGACCACATATTTCGCGAGTACGATGCAGACAAGAGAGGCACCC
TGGATTTCAACGAGTTCAAGCAACTAATGGCGGCAAGGCTCACGTACAAG
CTGTGTGACGTGAACGGAGACGGGTCCGTTACGAAGTCCGAGCTGAAAGA
TGCGCTTTGGTCACTGGGGCAGAATCCGACCGAGGAAGAGACCGACCACA
TATTTCGCGAGTACGATGCAGACAAGAGAGGCACCCTGGATTTCAACGAG
TTCAAGCAACTAATGGCGGCAAGGCTCACGTACAAGAACACACGGGCGGA
GTTTGAGGATGCTTTCCGAGCGATCGATCACAACAAGGATGGAAAGGTCG
ACATAACAGAGCTCAATAGGCTCATGGTTGCGACAGGGTTTGGACTGACC
AACCAAGAGTTGAAAGAAATTTTTGGCCTTTTCGACCGTGACGGCAGCGG
GAAGGTAGACATGGTAGAATTTTTGGACTATATGATGGACGCGTAAAACA
CACGGGCGGAGTTTGAGGATGCTTTCCGAGCGATCGATCACAACAAGGAT
GGAAAGGTCGACATAACAGAGCTCAATAGGCTCATGGTTGCGACAGGGTT
TGGACTGACCAACCAAGAGTTGAAAGAAATTTTTGGCCTTTTCGACCGTG
ACGGCAGCGGGAAGGTAGACATGGTAGAATTTTTGGACTATATGATGGAC
GCGTAA
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