mRNA_E-siliculosus-1a_M_contig87.16754.1 (mRNA) Ectocarpus siliculosus Ec864m_EcPH12_78m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_M_contig87.16754.1
Unique NamemRNA_E-siliculosus-1a_M_contig87.16754.1
TypemRNA
OrganismEctocarpus siliculosus Ec864m_EcPH12_78m male (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
Homology
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BLAST of mRNA_E-siliculosus-1a_M_contig87.16754.1 vs. uniprot
Match: D7FW28_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FW28_ECTSI)

HSP 1 Score: 86.3 bits (212), Expect = 3.240e-18
Identity = 37/39 (94.87%), Postives = 37/39 (94.87%), Query Frame = 2 
Query:  284 MQGSSWTRWTYPFDHENSKKCRKYWTAVRPRISKHRWWA 400
            MQGSSWTRWTYPFDHENSKKCRKYWTAV PRISKHR WA
Sbjct:    1 MQGSSWTRWTYPFDHENSKKCRKYWTAVPPRISKHRSWA 39
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BLAST of mRNA_E-siliculosus-1a_M_contig87.16754.1 vs. uniprot
Match: A0A6T9LUZ6_9EUKA (Hypothetical protein n=1 Tax=Haptolina ericina TaxID=156174 RepID=A0A6T9LUZ6_9EUKA)

HSP 1 Score: 81.6 bits (200), Expect = 7.720e-15
Identity = 49/123 (39.84%), Postives = 72/123 (58.54%), Query Frame = 1 
Query:  214 SGAFAAAGKVNKPLGQESFRRIRDARQQLDSVDVPLRSRKFEEVQEVLDSGATADLEASLVGLVKSNSLPADDRLAVGTIRRYGLAADALIALGGLSAAARDGDGGEARRMLTLTKESLNDIL 582
            +G+  AAGKVN  +   ++  +R AR  LD VD  +    ++    +L     A LE +L+ LV S  L  D+R ++GTI+RYG+ ADALI +GGL +A R GDG  A    +  K SL++I+
Sbjct:   62 AGSAKAAGKVNAAVAYAAYAELRAARSSLDKVDSLIGKSDYKAAASILTEPPLATLEVNLLTLVASPELGPDERKSIGTIKRYGVGADALIMMGGLGSALRSGDGSGASDYTSKAKASLDEIV 184
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BLAST of mRNA_E-siliculosus-1a_M_contig87.16754.1 vs. uniprot
Match: A0A835ZDP8_9STRA (Tetrapyrrole methylase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZDP8_9STRA)

HSP 1 Score: 73.9 bits (180), Expect = 6.220e-11
Identity = 41/65 (63.08%), Postives = 49/65 (75.38%), Query Frame = 1 
Query:  382 EASLVGLVKSNSLPADDRLAVGTIRRYGLAADALIALGGLSAAARDGDGGEARRMLTLTKESLND 576
            EA+L+ LV SN L  +D++AVGTIRRYG+AAD LI LGG+ AAA D D  EA R LTL K SL+D
Sbjct:  210 EANLLNLVNSNVLEREDKVAVGTIRRYGIAADILITLGGVRAAANDEDAREALRSLTLAKNSLDD 274
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BLAST of mRNA_E-siliculosus-1a_M_contig87.16754.1 vs. uniprot
Match: A0A8J2X3F4_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2X3F4_9STRA)

HSP 1 Score: 66.2 bits (160), Expect = 3.220e-9
Identity = 43/123 (34.96%), Postives = 68/123 (55.28%), Query Frame = 1 
Query:  220 AFAAAGKVNKPLGQESFRRIRDARQQLDSVDVPLRSRKFEEVQEVLDSGATADLEASLVGLVKSNSLPADDRLAVGTIRRYGLAADALIALGGLSAAARDGDGGEARRMLTLTKESLNDILTI 588
            A AA+ K +    Q++F  ++ AR  LD+VD PL           L +      E +   +V++  L  +D+ A+GTIRRYG+AAD +I  GGLS A  +GD  +A+  L   K SL++++ +
Sbjct:   75 AHAASKKYSAADAQKAFGELKAARAALDAVDKPLXXXXXXXXXXXLQAPPLVAFEENATIIVQAPVLTVEDKKAIGTIRRYGVAADVIIMTGGLSEALDNGDVRQAKSFLAKAKGSLDEVIGV 197
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BLAST of mRNA_E-siliculosus-1a_M_contig87.16754.1 vs. uniprot
Match: F0YPD1_AURAN (Uncharacterized protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YPD1_AURAN)

HSP 1 Score: 62.0 bits (149), Expect = 3.200e-7
Identity = 46/134 (34.33%), Postives = 73/134 (54.48%), Query Frame = 1 
Query:  187 AAAAVLAKPSGAFAAAGKVNKPLGQESFRRIRDARQQLDSVDVPLRSRKFEEVQEVLDSGATADLEASLVGLVKSNSLPADDRLAVGTIRRYGLAADALIALGGLSAAARDGDGGEARRMLTLTKESLNDILTI 588
            AAAA    P  A AA  K +    +++   I+  R+ LD +D  L    F+     L      + +A+++  +++  L A+D+ A+GTIRRYG+AAD +I  GGL  A RD D   A+  L   K SL+++++I
Sbjct:  195 AAAAAPLIPLAAAAAPKKYSADDAKQAIAGIKAGRKALDGIDALLAKGDFDGAAAALAPIDAFEDQATII--IQAPVLAAEDKKAIGTIRRYGVAADVIIMAGGLKEALRDEDARGAKSYLAKCKGSLDEVISI 326
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BLAST of mRNA_E-siliculosus-1a_M_contig87.16754.1 vs. uniprot
Match: A0A7S0NYT5_9EUKA (Hypothetical protein n=1 Tax=Calcidiscus leptoporus TaxID=127549 RepID=A0A7S0NYT5_9EUKA)

HSP 1 Score: 54.7 bits (130), Expect = 4.130e-5
Identity = 37/113 (32.74%), Postives = 60/113 (53.10%), Query Frame = 1 
Query:  256 GQESFRRIRDARQQLDSVDVPLRSRKFEEVQEVLDSGATADLEASLVGLVKSNSLPADD-RLAVGTIRRYGLAADALIALGGLSAAARDGDGGEARRMLTLTKESLNDILTIG 591
            GQ +F  I  AR  LD++D  +    F     +L     +    + + LV+S +LP  + + ++GT++R+GLAAD  I LG L  A  +GD   +R  L   K  L+++L +G
Sbjct:  107 GQLAFAEIVAARSALDTIDRLIAGSDFFSATALLGKRPLSTFTENALILVQSAALPDPEAKKSIGTVKRFGLAADVAILLGALQEALSNGDASGSRSFLKKAKAPLDEMLALG 219
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_M_contig87.16754.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a male vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
D7FW28_ECTSI3.240e-1894.87Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6T9LUZ6_9EUKA7.720e-1539.84Hypothetical protein n=1 Tax=Haptolina ericina Tax... [more]
A0A835ZDP8_9STRA6.220e-1163.08Tetrapyrrole methylase n=1 Tax=Tribonema minus Tax... [more]
A0A8J2X3F4_9STRA3.220e-934.96Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
F0YPD1_AURAN3.200e-734.33Uncharacterized protein n=1 Tax=Aureococcus anopha... [more]
A0A7S0NYT5_9EUKA4.130e-532.74Hypothetical protein n=1 Tax=Calcidiscus leptoporu... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_M_contig87contigE-siliculosus-1a_M_contig87:482245..486499 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a male vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec864m_EcPH12_78m male2021-02-24
Properties
Property NameValue
Start1
Stop1
Cds size120
Model size959
Exons4
Ec32 orthologEc-01_003140.1
Ec32 ortholog descriptionhypothetical protein
Hectar predicted targeting categoryno signal peptide or anchor
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_M_contig87.16754.1prot_E-siliculosus-1a_M_contig87.16754.1Ectocarpus siliculosus Ec864m_EcPH12_78m malepolypeptideE-siliculosus-1a_M_contig87 483247..483583 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622936335.094973-UTR-E-siliculosus-1a_M_contig87:482244..4827451622936335.094973-UTR-E-siliculosus-1a_M_contig87:482244..482745Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig87 482245..482745 -
1696251031.1748357-UTR-E-siliculosus-1a_M_contig87:482244..4827451696251031.1748357-UTR-E-siliculosus-1a_M_contig87:482244..482745Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig87 482245..482745 -
1622936335.1146224-UTR-E-siliculosus-1a_M_contig87:483191..4832461622936335.1146224-UTR-E-siliculosus-1a_M_contig87:483191..483246Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig87 483192..483246 -
1696251031.1856666-UTR-E-siliculosus-1a_M_contig87:483191..4832461696251031.1856666-UTR-E-siliculosus-1a_M_contig87:483191..483246Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig87 483192..483246 -
1622936335.1640062-UTR-E-siliculosus-1a_M_contig87:483583..4836821622936335.1640062-UTR-E-siliculosus-1a_M_contig87:483583..483682Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig87 483584..483682 -
1696251031.22795-UTR-E-siliculosus-1a_M_contig87:483583..4836821696251031.22795-UTR-E-siliculosus-1a_M_contig87:483583..483682Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig87 483584..483682 -
1622936335.3719478-UTR-E-siliculosus-1a_M_contig87:486315..4864991622936335.3719478-UTR-E-siliculosus-1a_M_contig87:486315..486499Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig87 486316..486499 -
1696251031.2414048-UTR-E-siliculosus-1a_M_contig87:486315..4864991696251031.2414048-UTR-E-siliculosus-1a_M_contig87:486315..486499Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig87 486316..486499 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622936335.1320076-CDS-E-siliculosus-1a_M_contig87:483246..4833011622936335.1320076-CDS-E-siliculosus-1a_M_contig87:483246..483301Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig87 483247..483301 -
1696251031.1961193-CDS-E-siliculosus-1a_M_contig87:483246..4833011696251031.1961193-CDS-E-siliculosus-1a_M_contig87:483246..483301Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig87 483247..483301 -
1622936335.1483781-CDS-E-siliculosus-1a_M_contig87:483518..4835831622936335.1483781-CDS-E-siliculosus-1a_M_contig87:483518..483583Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig87 483519..483583 -
1696251031.206027-CDS-E-siliculosus-1a_M_contig87:483518..4835831696251031.206027-CDS-E-siliculosus-1a_M_contig87:483518..483583Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig87 483519..483583 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_M_contig87.16754.1

>prot_E-siliculosus-1a_M_contig87.16754.1 ID=prot_E-siliculosus-1a_M_contig87.16754.1|Name=mRNA_E-siliculosus-1a_M_contig87.16754.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=polypeptide|length=40bp
MQGSSWTRWTYPFDHENSKKCRKYWTAVRPRISKHRWWA*
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mRNA from alignment at E-siliculosus-1a_M_contig87:482245..486499-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_M_contig87.16754.1 ID=mRNA_E-siliculosus-1a_M_contig87.16754.1|Name=mRNA_E-siliculosus-1a_M_contig87.16754.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=mRNA|length=4255bp|location=Sequence derived from alignment at E-siliculosus-1a_M_contig87:482245..486499- (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
CTGCATGACACTACAGAGGGCATGCACATGTTCATCGGACAACAGAGAAG
CAACATGTTGTTGCGTCTAGGGACGCTTGCTCTCTTGGCCTACACCAGCC
ATGGGTTCGTCATGAGTCTGGGCTGTGGTCCCAGCGAGCGAGCGTTGAGC
AGACGCGAAGCGATTTCAAAGGTTTCCCTGGCAGGTGACGAATCAAAGCA
TCAAACCTTGCGACGTTGCGAGAACGACTGACTCCGGTACTCGGTCAAGC
GTAGATCCTATCTTGCTGAGTCTTATCGTTGTCATCATTTGTTCAAGGGT
GATGGCTGCCTCATGAAAAGCGTGGTGAAGTTTAGCTCGTTGTCTCTCAA
GGTAGTTGTACTTTCTATCCAACAATCAGAAGTTGCACATGTTTGTTGGG
GCAACAACAGCAGCAGCAGCAGCTGCACACAAACAGCCTGCTGAGACATA
CATGTCTACACGGCACCATGATGATGGCAGAAAGGAATGAACGAATGAAC
CGCATGGATGGTTGTGAGAAAGAGTGTGTGGTAGTAATTTAGTAGATCTT
GCTGTCGATGAAGGGCAAAAAAATGGTCTTCATTTCTAGTTTTTTCTTTA
TAGGGTCAACATCGGCGTGTAGTTCTGGGCCCCCGCAACGGCTGAAGGGA
CGGCTCATGTCGGGTTTGAGTAGGTTGAGCATCAATTGACGAATCGTAAG
CAGCGCAACCAGACACACAAGTTTGTGTCAGACACCGAAGAACTTCCAAC
AATCATCTATGTTTTAGAGGGGTAGAGCAGGGTAGATACCCATCTCCCAC
CAATCACATTTCGATTGTCAGACATTTTGTAAGGCAGAGACAGATGATTC
GATGGGCACGACGCTCGTCGGGGAGAATCCCCGAAATCCTCTTGAAGGAC
GCAGCACAGCAGTACCACACACACCACGTAGACAACATACGAGACAATCA
GCACAGCAGCACACGAGGATGCTGTGGGCCAAGGGCAGCCAAAGGCACAC
ATCATAGGAGGCGTTCCCTATGACCCTTACAGCCCGTTGCCTGCTGTCGG
CAGCACAAGCGAGTGACGACACAAGTCAATCTGCGGTCCGTCTACGTCAT
CCTCTATCTACCGATCTTTGGAGCTTCCCACCCAGACGCATCCCCACAAC
AGAGACAACGAGACGTACACACTGCTGTAGGTCAAGGCCGGCCAAAGGCA
CACATCGGCTGGCGTCGGAAGCTATTTTTAGAGCCCGAGGCCAGCTGCTA
GCAGCGTAAGCGAGGGAACATGCGAGTGCACACACGTCAAATCTGTCTGC
GGTGTCTTCGTCTGCAGGCCGATCTTTGCGTCCACAACAGACGCACCCCT
GAAACAGAGACAAGTGAGACGTACACAACGCTGTAGGCCAAGGGCGGCCA
AATACAAATACACACATCGTAGGAGGCGTCGGAGCGATTTTTAGAGCCCG
TTGCCCGCTGCGAAGCGAGGAAAGCAAATGCACACCCATGCACAGCAGCA
GCAGAAACAACAGCAGCTGCTGCAGCTACATGCGCTCTCATTCATCCCCT
GACTGACTGGTGGTGTCGGGTGAGGTGTGAGCGTGTGCACTGGTGACCTT
TGTGGTGGCGTAGGCTCCATCCATATCTATCGAAGCTTGCTTTGGTGTCT
CTCTCCGCCCCTTCTATTGGCGCTTCGCGCGGAATAATTTAGACCCCCGT
GTATGGGAGGTGAGCCGAATCGTAGGAGTTCGATGCCTCTCCCACGCAAA
CCTGAACCTTCGGTCAATCTTTTTTTCTGGCGCCCATTTTTGGTATGCCT
ATCTTTACGCTTCCTGATTTTTAACTATTAACTGCTGTCTGTCTGTGTAA
TACGTAGTGTGTGTGCTAACTTCTGCTGTGTACGTGCGAAGTCGATAGTA
TTCGTTACGCGATGACTCGCCGTCCCCAGAGTATCTGCGAGGATATGCTG
AATCACAGCTAACATAATTTTTTTCACCATCATCCATGTACATATCTCTA
CGGAAAAGGAAGTGGGCAATGGGCCGATGACTGACTCCCTCTTACGTGCA
CTTCGCAGTACTCGAGTGTTTGTTTTTTGGTGTTTTCCTTGCCCAGTTTT
TACTTGTTTGTTCTTTTTTTTTGCCAGGTTCGATCGGCATCATGTACCTA
CCATACAGCTGTTTCTCTCTCTCTCGTTGATGCAGTAGCAGGCCACCGTC
AGAATTGAATTGAACAAACGGTTGCCAGTTTCATAAATGCCTATCTATAT
GCTTCCTCATTCTTCTGTAACTTCTGTCTGTCTGTGTAATATGTAGTGCT
TGCTAAGTACTGCTGTGTACGTACGAAGTCGATAGTATTCGTTACGCGAT
GACTCGCCGTCCCCAGAGTATCTGCGAGGATATACTGAATCACAGCTAAG
ATACGTTTTTTCACTATCATCCATGTAGTCATATCTCTAAAGAAAAGGAA
GTGGCCGATGGGATGACTGACTCCCTCTTACGTGCACTTCGAAGTACTCG
AGTGTTTATTTTTTGGGGCTTTTTTTCCTTGCCCAGCTTTTACTTTTTCG
TTCTCTTTTTTGTTCTTTTCCTTGTTTGTTCCTTTTTTTGGCCAGGTTCG
ATATGCTCGGCATCATGTCCCTACCATGTACGAAGTAGCTGTTTCTCTCT
CTCGTCCATGCAGTAGCAGGCCACCGTAATAATTGAATTTGAACGAACGG
TTTCCAGTTTCATAAATGCCCGTGCTGTCGAGCGTAGGGAAAGAAGAGTA
GCTGGTGCTCGCATCGATTGAACCTTTGAAAACTGCCGATCCTCCCAAAC
CGCATCGTTCTAAACAGGGGCAGCCGCTGCTGTACTGGCCAAGCCGAGCG
GTGCGTTCGCCGCCGCAGGCAAAGTCAACAAGCCGCTGGGACAGGAATCA
TTCCGGAGGATCAGGGATGCAAGGCAGCAGTTGGACTCGGTGGACGTACC
CCTTCGATCACGAAAATTCGAAGAAGTGCAGGTACGATTTTGCAGCACCT
CTCATGAGGAGATATGGTGCTGACCGACGACAGCATTTTGTTCATGCACC
CACAGCAGTACGGATATTGGAAGGTTCTTGAGCGGAGACCAGAACGGTGC
TAAATGTGAAGAAAATAACAACAACAACCACAACAACAATCACAGCCCAC
TTGTTTGTTTTTGATGCGTGTTGAATATCATTTCGTCACTGCGTGCAGGA
AGTATTGGACAGCGGTGCGACCGCGGATCTCGAAGCATCGCTGGTGGGCC
TAGTCAAGTCCAACTCATTGCCGGCTGACGACAGGCTTGCCGTGGGCACT
ATTCGAAGGTGTATTTGCAGTTGCTTTTGGTCGATATGTAGCCCCCTTTG
CGGTGTGCCTATGCCGTTGCAGGATCAGCCCTGTTCGTCCGCGACCTACA
GCAATACATCCACTTTGATGCGATGCTTCTTGTGTGGATAGACACGATGC
CCCAACAAGCGCAATGTAGCATGTTGTCATTTTATTTATTTTTCACCGGC
GTTGGTGTGTTAACCGTCCCACCTTCATCGAAATATAGAATCACGTGTGA
GGTTGACCTCTTAAAAAGTCAGCAGTGTGTCCCGACAGGCGGACACCGGC
ACGATACATACGCGCTAAACACCAACACCTGCGAGTCTTCTTTTCTTTCA
CCTTGCCCGCTGCTGTGACCGCGACAAGCGACTCCAAGGTTTCCTTGGTC
TCGACATGAGTTGGGCTGAAAAAAGAGTTGACGCTAATTATTTTTTTTTC
CCAGGTACGGACTCGCCGCGGACGCGCTGATAGCGCTCGGCGGGCTCTCA
GCTGCGGCCCGCGACGGGGACGGTGGGGAGGCCCGCCGGATGCTCACGCT
AACCAAGGAATCGCTCAATGATATCCTCACGATTGGGGTCAACAACGACC
TCAACAAAACGAACTGATTAGACAGACTGGGCAGCAACCCCGTGTTGTTT
GTCGATGTACGATGGTTTGGGGCTTGCCCCTGTGTGATTACGGACATTTT
TTCTTTCGTGCATCAGTCCAGGACTGCTGCTGTGTCTCACAAGAGAGAGG
GATCTACTGTACTTGGCGCCGAAAAGGATCTTCGCTGCACTGCTGTTTTT
TGTGTTTCCCGCCGGCTTAACGGGGATTGCTACGTAGGCCGTAGGTGTTT
TGCGCCTACAGCAACAGCCCGAAGGAGTTGGTACGGTGGGCCTGTTGATG
TGTACAAATAAATGTCGCTCGAGTGGAACAGAGAAATTAGTTACAAATAA
TTCAT
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Coding sequence (CDS) from alignment at E-siliculosus-1a_M_contig87:482245..486499-

>mRNA_E-siliculosus-1a_M_contig87.16754.1 ID=mRNA_E-siliculosus-1a_M_contig87.16754.1|Name=mRNA_E-siliculosus-1a_M_contig87.16754.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=CDS|length=240bp|location=Sequence derived from alignment at E-siliculosus-1a_M_contig87:482245..486499- (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
ATGCAAGGCAGCAGTTGGACTCGGTGGACGTACCCCTTCGATCACGAAAA
TTCGAAGAAGTGCAGATGCAAGGCAGCAGTTGGACTCGGTGGACGTACCC
CTTCGATCACGAAAATTCGAAGAAGTGCAGGAAGTATTGGACAGCGGTGC
GACCGCGGATCTCGAAGCATCGCTGGTGGGCCTAGGAAGTATTGGACAGC
GGTGCGACCGCGGATCTCGAAGCATCGCTGGTGGGCCTAG
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