prot_E-siliculosus-1a_F_contig1194.1234.1 (polypeptide) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview


Name	mRNA_E-siliculosus-1a_F_contig1194.1234.1
Unique Name	prot_E-siliculosus-1a_F_contig1194.1234.1
Type	polypeptide
Organism	Ectocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Sequence length	347

Homology

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BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot
Match: D8LGV3_ECTSI (2OG-Fe(II) oxygenase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LGV3_ECTSI)

HSP 1 Score: 556 bits (1433), Expect = 6.720e-198
Identity = 297/346 (85.84%), Postives = 307/346 (88.73%), Query Frame = 0

Query:    5 EGKRGRSSRPNEQVGAKRRWGGSSSSSQPKAPATATCSCETFPLQSGTGKLRFWPSLLTDAAQRRLFEELTVNPNLLPLDPKTGCSGGGXXX----DGSPGLREDAAARGRWMQRPIKLFGREIPQPRLTCFYGRRGVSYRRVLFEYSGKTLEATPWNGVPAIQEILAAAEAAAGVDPGYFNCVLLNWYRDGADYMGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDHTQKMEFVLGGGSLLLMEGNTQEHWQHRVPKRTAKEEREIRQSPQERREAKALSPLLLSWSGQGGKESSSLNTGESAGGNGSIAGGRGGSSLLTSSAGARGGRINLTFRRIKEGVS 346
            EGKRGR+SRPNEQVGAKRRW GSSS             CETF LQSGTGKLRFWPSLLTDAAQRRLFEELTV+P+L PLDPK G S   XXX     G+PGLRED AARGRWMQRPIKLFGREIPQPRLTCFYGR GVSYR     YSGKTLEATPW+GVPAIQEILAAA AAAGVDPGYFNCVLLNWYRDGADYMGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDH QK+EF+LGGGSLLLMEG+TQEHWQHRVPKRTAKEERE+RQSPQERREAKALSPLLLSWSGQGGKESSSL TGES GGNGSI GGR GSSLLTSSAGARGGRINLTFRR+KEGVS
Sbjct:   10 EGKRGRASRPNEQVGAKRRWSGSSSXXXXXXXXXXXXXCETFHLQSGTGKLRFWPSLLTDAAQRRLFEELTVDPDLRPLDPKAGSSXXXXXXXXXXSGNPGLREDGAARGRWMQRPIKLFGREIPQPRLTCFYGRTGVSYR-----YSGKTLEATPWDGVPAIQEILAAAGAAAGVDPGYFNCVLLNWYRDGADYMGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDHAQKVEFILGGGSLLLMEGSTQEHWQHRVPKRTAKEEREVRQSPQERREAKALSPLLLSWSGQGGKESSSLKTGES-GGNGSITGGRVGSSLLTSSAGARGGRINLTFRRVKEGVS 349

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BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot
Match: A0A6H5J9B1_9PHAE (Fe2OG dioxygenase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J9B1_9PHAE)

HSP 1 Score: 258 bits (659), Expect = 2.580e-83
Identity = 140/151 (92.72%), Postives = 144/151 (95.36%), Query Frame = 0

Query:  196 MGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDHTQKMEFVLGGGSLLLMEGNTQEHWQHRVPKRTAKEEREIRQSPQERREAKALSPLLLSWSGQGGKESSSLNTGESAGGNGSIAGGRGGSSLLTSSAGARGGRINLTFRRIKEGVS 346
            MGWHSDDEKELEKGAAIASVSLGAGRRF+LR +KD TQKMEFVLGGGSLLLMEG+TQEHWQHRVPKRTAKEEREI QSPQERR+AKALSPLLLSWSGQGGK  SSLNTGES GGNGSIAGG GGSSLLTSSAGARGGRINLTFRRIKEGVS
Sbjct:    1 MGWHSDDEKELEKGAAIASVSLGAGRRFELRGRKDRTQKMEFVLGGGSLLLMEGSTQEHWQHRVPKRTAKEEREILQSPQERRKAKALSPLLLSWSGQGGKVLSSLNTGES-GGNGSIAGGPGGSSLLTSSAGARGGRINLTFRRIKEGVS 150

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BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot
Match: A0A355CG02_9CYAN (Alpha-ketoglutarate-dependent dioxygenase AlkB n=2 Tax=unclassified Microcoleaceae TaxID=2053578 RepID=A0A355CG02_9CYAN)

HSP 1 Score: 150 bits (378), Expect = 2.230e-40
Identity = 80/164 (48.78%), Postives = 100/164 (60.98%), Query Frame = 0

Query:  102 LREDAAARGRWMQRPIKLFGREIPQPRLTCFYGRRGVSYRRVLFEYSGKTLEATPWNGVPAIQEILAAAEAAAGVDPGYFNCVLLNWYRDGADYMGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDHTQKMEFVLGGGSLLLMEGNTQEHWQHRVPKRTAK 265
            L  D   R  W Q PIK+FG+ + QPRLT +YG +        + YSG T++  PWN     + +L   +    +    FN VLLN YR+G DYMGWHSDDE+ L KGA IASVS G  RRF LRR+ +H  K+E  LG G  L+M G+TQ+ WQH VPK TAK
Sbjct:   35 LLHDLLDRIEWKQEPIKIFGKSVMQPRLTAYYGTQA-------YTYSGVTMQPLPWN-----EPLLQIKQEIEPLVNTQFNGVLLNLYRNGQDYMGWHSDDERNLAKGAIIASVSFGETRRFILRRRDNHQIKVELNLGDGDFLVMGGDTQKFWQHHVPK-TAK 185

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BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot
Match: A0A352A9Z2_9CYAN (Alpha-ketoglutarate-dependent dioxygenase AlkB n=1 Tax=Cyanobacteria bacterium UBA9273 TaxID=2055791 RepID=A0A352A9Z2_9CYAN)

HSP 1 Score: 148 bits (374), Expect = 7.550e-40
Identity = 86/216 (39.81%), Postives = 111/216 (51.39%), Query Frame = 0

Query:   46 FPLQSGTGKLRFWPSLLTDAAQRRLFEELTVNPNLLPLDPKTGCSGGGXXXDGSPGLREDAAARGRWMQRPIKLFGREIPQPRLTCFYGRRGVSYRRVLFEYSGKTLEATPWNGVPAIQEILAAAEAAAGVDPGYFNCVLLNWYRDGADYMGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDHTQKMEFVLGGGSLLLMEGNTQEHWQHRVPK 261
             PLQ     + F+PSLL +    R+F +L+   N                                W Q  IK++GR +PQPRLT +Y   G+SY      YSG T+  +PW     + ++ A AEA +GV    FN VLLN YRDG D MGWHSDDE EL     I S+SLG  RRF LR + +   K +  L  GS LLM+G TQ +WQH++PK
Sbjct:    4 IPLQLPDADIVFYPSLLDEQESDRIFTQLSQTLN--------------------------------WRQDWIKIYGRSMPQPRLTAWYANPGISYT-----YSGITMHPSPWTHT--LLDLKAKAEAVSGV---VFNSVLLNLYRDGNDSMGWHSDDEPELGDNPVIGSLSLGGTRRFMLRHRTEKALKHQLELTSGSFLLMQGTTQHYWQHQIPK 177

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BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot
Match: A0A0M1K085_9CYAN (2OG-Fe(II) oxygenase n=2 Tax=Planktothricoides TaxID=132607 RepID=A0A0M1K085_9CYAN)

HSP 1 Score: 148 bits (373), Expect = 1.190e-39
Identity = 76/150 (50.67%), Postives = 91/150 (60.67%), Query Frame = 0

Query:  112 WMQRPIKLFGREIPQPRLTCFYGRRGVSYRRVLFEYSGKTLEATPWNGVPAIQEILAAAEAAAGVDPGYFNCVLLNWYRDGADYMGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDHTQKMEFVLGGGSLLLMEGNTQEHWQHRVPK 261
            W   PIK+FG+ + QPRLT +YG +        + YSG T++  PWN       +L   E    +    FN VLLN YRDG DYMGWHSDDEKEL KG AIAS+SLG  RRF  RRK +H  K E  L  G  L+M G+TQ+ WQH VPK
Sbjct:   45 WKHEPIKIFGKSVLQPRLTAYYGTKA-------YTYSGVTMQPLPWNA-----PLLQIKEKIEPLVNTQFNGVLLNLYRDGQDYMGWHSDDEKELVKGGAIASLSLGETRRFIFRRKDNHQIKFELKLADGDFLVMGGDTQKFWQHYVPK 182

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BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot
Match: UPI001684B391 (alpha-ketoglutarate-dependent dioxygenase AlkB n=2 Tax=Planktothrix TaxID=54304 RepID=UPI001684B391)

HSP 1 Score: 148 bits (373), Expect = 1.190e-39
Identity = 76/150 (50.67%), Postives = 92/150 (61.33%), Query Frame = 0

Query:  112 WMQRPIKLFGREIPQPRLTCFYGRRGVSYRRVLFEYSGKTLEATPWNGVPAIQEILAAAEAAAGVDPGYFNCVLLNWYRDGADYMGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDHTQKMEFVLGGGSLLLMEGNTQEHWQHRVPK 261
            W Q PIK+FG+ + QPRLT +YG R        + YSG T++  PWN +P +Q I    E         FN VLLN YRDG DY+GWHSDDEKEL KG  I S+SLG  RRF  RR+ +H  K+E  L  G  L+M G TQ+ WQH VPK
Sbjct:   45 WKQEPIKIFGKSVLQPRLTAYYGDRA-------YTYSGVTMQPLPWN-LPLLQ-IKEKIEPLVNTQ---FNGVLLNLYRDGQDYIGWHSDDEKELAKGGVIGSLSLGETRRFIFRRRDNHQNKIELTLSDGDFLIMGGETQKFWQHHVPK 182

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BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot
Match: A0A291R0S7_9BACT (Alpha-ketoglutarate-dependent dioxygenase AlkB n=2 Tax=Chitinophaga caeni TaxID=2029983 RepID=A0A291R0S7_9BACT)

HSP 1 Score: 147 bits (372), Expect = 1.680e-39
Identity = 77/150 (51.33%), Postives = 93/150 (62.00%), Query Frame = 0

Query:  112 WMQRPIKLFGREIPQPRLTCFYGRRGVSYRRVLFEYSGKTLEATPWNGVPAIQEILAAAEAAAGVDPGYFNCVLLNWYRDGADYMGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDHTQKMEFVLGGGSLLLMEGNTQEHWQHRVPK 261
            W  +PIK+FG+EI QPRLT FYG  G SY      YSG    A PW+  PA+  I    E A    P  FNCVL N YRDG D MGWH D+E+ L     IASVSLGA R FQ+R     + ++ F LG GSLL+M G TQ++W+H+VPK
Sbjct:   40 WKHKPIKIFGKEIMQPRLTAFYGDPGTSYT-----YSGLQQAAIPWH--PALSGIKRKIETAC---PEPFNCVLANLYRDGQDSMGWHRDNEESLGACPTIASVSLGASRSFQMRDYATKSTRLSFELGNGSLLIMSGETQQYWEHQVPK 179

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BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot
Match: A0A8J7K6S7_9GAMM (Alpha-ketoglutarate-dependent dioxygenase AlkB n=1 Tax=Pontibacterium sp. N1Y112 TaxID=2781979 RepID=A0A8J7K6S7_9GAMM)

HSP 1 Score: 147 bits (370), Expect = 2.870e-39
Identity = 79/150 (52.67%), Postives = 93/150 (62.00%), Query Frame = 0

Query:  112 WMQRPIKLFGREIPQPRLTCFYGRRGVSYRRVLFEYSGKTLEATPWNGVPAIQEILAAAEAAAGVDPGYFNCVLLNWYRDGADYMGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDHTQKMEFVLGGGSLLLMEGNTQEHWQHRVPK 261
            W Q  I+LFG++ P PRL CF G  G++YR     YS   L+A  W+  PAIQ +    EA   +  G FN VL+N YRDG D MGWHSDDE EL +   IAS+SLG  RRF LRRK +H  K E VL  GSLLLM G  Q  WQH VP+
Sbjct:   38 WQQDRIQLFGKKHPIPRLQCFQGEPGITYR-----YSNLCLDAAAWH--PAIQCLREQLEA---LGYGSFNAVLMNLYRDGLDSMGWHSDDEAELGENPVIASISLGEARRFLLRRKDNHATKQELVLEHGSLLLMSGELQHFWQHSVPR 177

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BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot
Match: A0A8B5WG54_9GAMM (Alpha-ketoglutarate-dependent dioxygenase AlkB n=1 Tax=Wenzhouxiangella sp. TaxID=1979961 RepID=A0A8B5WG54_9GAMM)

HSP 1 Score: 146 bits (369), Expect = 3.920e-39
Identity = 84/164 (51.22%), Postives = 103/164 (62.80%), Query Frame = 0

Query:  102 LREDAAARGRWMQRPIKLFGREIPQPRLTCFYGRRGVSYRRVLFEYSGKTLEATPWNGVPAIQEILAAAEAAAGVDPGYFNCVLLNWYRDGADYMGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDHTQKMEFVLGGGSLLLMEGNTQEHWQHRVPKRTAK 265
            LRE       W QRPI +FGR++ QPRLT F+G   V+YR     YSG+TL A PW G   + EI    EA  G+    FN VL N YRDG D MGWH+DDE EL +   IAS+SLGA RRF LR ++    ++E VL  GSLL+M G+ Q  WQH+VP RTA+
Sbjct:   24 LREKMLGSLDWDQRPIFMFGRKVLQPRLTAFHGDDHVAYR-----YSGQTLHARPWTG--DLSEIRERLEAVLGIR---FNSVLCNLYRDGRDSMGWHADDEVELGQNPVIASISLGAARRFLLRARQGD-DRLELVLRNGSLLVMAGDCQHFWQHQVP-RTAR 175

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BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot
Match: A0A7C3ZNA2_9CYAN (Alpha-ketoglutarate-dependent dioxygenase AlkB n=1 Tax=Planktothricoides sp. SpSt-374 TaxID=2282167 RepID=A0A7C3ZNA2_9CYAN)

HSP 1 Score: 145 bits (366), Expect = 8.620e-39
Identity = 79/154 (51.30%), Postives = 96/154 (62.34%), Query Frame = 0

Query:  112 WMQRPIKLFGREIPQPRLTCFYGRRGVSYRRVLFEYSGKTLEATPWNGVPAIQEILAAAEAAAGVDPGYFNCVLLNWYRDGADYMGWHSDDEKELEKGAAIASVSLGAGRRFQLRRKKDHTQKMEFVLGGGSLLLMEGNTQEHWQHRVPKRTAK 265
            W Q PIK+FG+ + QPRLT +YG +G       + YSG  +E  PWN  P +Q I A  E     +   FN VLLN YRDG DY+GWHSDDEK+L KGA I S+SLG  RRF  RR+ +H  + E  L  G  L M G+TQ+ WQH VPK TAK
Sbjct:   31 WKQEPIKIFGKSVLQPRLTAYYGTQG-------YTYSGVKMEPLPWNE-PLLQ-IKAKIEPLVDTE---FNGVLLNLYRDGQDYIGWHSDDEKDLAKGAVIGSLSLGDSRRFIFRRRDNHQIRAELNLSDGDFLTMGGDTQKFWQHHVPK-TAK 171

The following BLAST results are available for this feature:

BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot

BLAST of mRNA_E-siliculosus-1a_F_contig1194.1234.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
D8LGV3_ECTSI	6.720e-198	85.84	2OG-Fe(II) oxygenase n=1 Tax=Ectocarpus siliculosu...	[more]
A0A6H5J9B1_9PHAE	2.580e-83	92.72	Fe2OG dioxygenase domain-containing protein n=1 Ta...	[more]
A0A355CG02_9CYAN	2.230e-40	48.78	Alpha-ketoglutarate-dependent dioxygenase AlkB n=2...	[more]
A0A352A9Z2_9CYAN	7.550e-40	39.81	Alpha-ketoglutarate-dependent dioxygenase AlkB n=1...	[more]
A0A0M1K085_9CYAN	1.190e-39	50.67	2OG-Fe(II) oxygenase n=2 Tax=Planktothricoides Tax...	[more]
UPI001684B391	1.190e-39	50.67	alpha-ketoglutarate-dependent dioxygenase AlkB n=2...	[more]
A0A291R0S7_9BACT	1.680e-39	51.33	Alpha-ketoglutarate-dependent dioxygenase AlkB n=2...	[more]
A0A8J7K6S7_9GAMM	2.870e-39	52.67	Alpha-ketoglutarate-dependent dioxygenase AlkB n=1...	[more]
A0A8B5WG54_9GAMM	3.920e-39	51.22	Alpha-ketoglutarate-dependent dioxygenase AlkB n=1...	[more]
A0A7C3ZNA2_9CYAN	8.620e-39	51.30	Alpha-ketoglutarate-dependent dioxygenase AlkB n=1...	[more]

Pages

InterPro

Analysis Name: InterProScan on OGS1.0 of Ectocarpus siliculosus 1a female
Date Performed: 2022-09-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 108..339 e-value: 2.2E-30 score: 106.3
None	No IPR available	PANTHER	PTHR31573	ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 2	coord: 106..268
None	No IPR available	PANTHER	PTHR31573:SF1	DNA OXIDATIVE DEMETHYLASE ALKBH2	coord: 106..268
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 108..342
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 180..280 score: 12.234

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
E-siliculosus-1a_F_contig1194	contig	E-siliculosus-1a_F_contig1194:23139..25936 -

Analyses

This polypeptide is derived from or has results from the following analyses


Analysis Name	Date Performed
InterProScan on OGS1.0 of Ectocarpus siliculosus 1a female	2022-09-29
Diamond blastp: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90	2022-09-16
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female	2021-02-24

Relationships

This polypeptide derives from the following mRNA feature(s):


Feature Name	Unique Name	Species	Type	Position
mRNA_E-siliculosus-1a_F_contig1194.1234.1	mRNA_E-siliculosus-1a_F_contig1194.1234.1	Ectocarpus siliculosus Ec863f_EcPH12_90f female	mRNA	E-siliculosus-1a_F_contig1194 22577..26004 -

Sequences

The following sequences are available for this feature:

polypeptide sequence

polypeptide sequence

>prot_E-siliculosus-1a_F_contig1194.1234.1 ID=prot_E-siliculosus-1a_F_contig1194.1234.1|Name=mRNA_E-siliculosus-1a_F_contig1194.1234.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=347bp
MSSAEGKRGRSSRPNEQVGAKRRWGGSSSSSQPKAPATATCSCETFPLQS
GTGKLRFWPSLLTDAAQRRLFEELTVNPNLLPLDPKTGCSGGGGGGDGSP
GLREDAAARGRWMQRPIKLFGREIPQPRLTCFYGRRGVSYRRVLFEYSGK
TLEATPWNGVPAIQEILAAAEAAAGVDPGYFNCVLLNWYRDGADYMGWHS
DDEKELEKGAAIASVSLGAGRRFQLRRKKDHTQKMEFVLGGGSLLLMEGN
TQEHWQHRVPKRTAKEEREIRQSPQERREAKALSPLLLSWSGQGGKESSS
LNTGESAGGNGSIAGGRGGSSLLTSSAGARGGRINLTFRRIKEGVS*

Annotated Terms

The following terms have been associated with this polypeptide:

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR027450	AlkB-like