mRNA_E-siliculosus-1a_F_contig1477.2786.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig1477.2786.1
Unique NamemRNA_E-siliculosus-1a_F_contig1477.2786.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig1477.2786.1 vs. uniprot
Match: A0A6H5K094_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K094_9PHAE)

HSP 1 Score: 365 bits (937), Expect = 1.820e-118
Identity = 238/290 (82.07%), Postives = 239/290 (82.41%), Query Frame = 1 
Query:  112 MEQVFFRLHVRFVTTVTRLLSPKTRRLLEYVTLANAFVLVTSLAWLHTRFVNPKGRQGSAVSCLPAALEKAGVDPAQLHVLQIHIRQPPDDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAEQQVSDSRRHSGGATVDAGLEISGLGGGESGDGSATCT--SAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLLPPRLDSPPQSPEIFYSMKLVTGKDAVGTLSGPGTLGIERCVVKGGDGVDGNNPAGXXXXXXXICGAVVEEKRPTLVQTLMDAFSWGGRKGET 975
            MEQVFFRLHVRFVTTVTRLLSPKTRRLLEYVTLANAF+LVTSLAWLHTRFVNPKG QGSAVSCLPAALEKAGVDPAQLHVLQIHIRQPPDDXXXXXXXXXX                      AEQQVSDSRRHSGGA  DAGL ISGLGGGESG  SAT T  SA                XXXXXXXXXXXXX LLPPRLDSPP SPEIFYSMKLVTGKDAVGTLSGPGTLGIERCVVKGGDGVDGNNPAGXXXXXXXICGAVVEEKRP LVQTLMDAFSWGG KGET
Sbjct:    1 MEQVFFRLHVRFVTTVTRLLSPKTRRLLEYVTLANAFLLVTSLAWLHTRFVNPKGSQGSAVSCLPAALEKAGVDPAQLHVLQIHIRQPPDDXXXXXXXXXXKNLMSFPDGSSRIDDSSRSGTNAEQQVSDSRRHSGGAAGDAGLGISGLGGGESGYASATGTAASACTGSGEEEGAAGTMGSXXXXXXXXXXXXXWLLPPRLDSPPLSPEIFYSMKLVTGKDAVGTLSGPGTLGIERCVVKGGDGVDGNNPAGXXXXXXXICGAVVEEKRPALVQTLMDAFSWGG-KGET 289
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BLAST of mRNA_E-siliculosus-1a_F_contig1477.2786.1 vs. uniprot
Match: D8LLK2_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LLK2_ECTSI)

HSP 1 Score: 318 bits (814), Expect = 2.840e-96
Identity = 237/297 (79.80%), Postives = 240/297 (80.81%), Query Frame = 1 
Query:  112 MEQVFFRLHVRFVTTVTRLLSPKTRRLLEYVTLANAFVLVTSLAWLHTRFVNPKGRQGSAVSCLPAALEKAGVDPAQLHVLQIHIRQPPDDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAEQQVSDSRRHSGG------ATVDAGLEISGLGGGESGDGSATCTSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX---LLPPRLDSPPQSPEIFYSMKLVTGKDAVGTLSGPGTLGIERCVVKGGDGVDGNNPAGXXXXXXXICGAVVEEKRPTLVQTLMDAFSWGGRKGET 975
            MEQVFFRLHVRFVTTVTRLLSPKTRRLLEYVTLANAF+LVTSLAWLHTRFVNPKGRQGSAVSCLPAALEKAGVDPAQLHVLQIHIRQPPDDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX                    A  DAGL +SGLG       S T T+A                     XXXXXXXXX   LLPPRLDSPPQSPEIFYSMKLVTGKDAVGTLSGPGTLGIERCVVKGGDGVDGNNPAGXXXXXXXICGAVVEEKRPTLVQTLMDAFSWGG KGET
Sbjct:    1 MEQVFFRLHVRFVTTVTRLLSPKTRRLLEYVTLANAFLLVTSLAWLHTRFVNPKGRQGSAVSCLPAALEKAGVDPAQLHVLQIHIRQPPDDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAAADAGLGMSGLGXXXXXXXSVTGTTAATACTGSGEEVGAAGAIGSESXXXXXXXXXXXXLLPPRLDSPPQSPEIFYSMKLVTGKDAVGTLSGPGTLGIERCVVKGGDGVDGNNPAGXXXXXXXICGAVVEEKRPTLVQTLMDAFSWGG-KGET 296
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BLAST of mRNA_E-siliculosus-1a_F_contig1477.2786.1 vs. uniprot
Match: A0A7S2WVH7_9STRA (Hypothetical protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WVH7_9STRA)

HSP 1 Score: 53.9 bits (128), Expect = 7.560e-5
Identity = 29/54 (53.70%), Postives = 37/54 (68.52%), Query Frame = 1 
Query:  112 MEQVFFRLHVRFVTTVTRLLSPKTRRLLEYVTLANAFVLVTSLAWLHTRFVNPK 273
            MEQVFFRLHVR V T+TR  SP  RR++EY  LA +  L + +  LHTR + P+
Sbjct:    1 MEQVFFRLHVRCVRTITRACSPTFRRVVEYGLLALSVGLCSLVVALHTRHIAPR 54
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig1477.2786.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
A0A6H5K094_9PHAE1.820e-11882.07Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LLK2_ECTSI2.840e-9679.80Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A7S2WVH7_9STRA7.560e-553.70Hypothetical protein (Fragment) n=1 Tax=Rhizochrom... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig1477contigE-siliculosus-1a_F_contig1477:27779..30305 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start0
Stop0
Cds size975
Model size976
Exons4
Hectar predicted targeting categoryother localisation
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
COG Functional cat.S
Ec32 orthologEc-04_005490.1
Ec32 ortholog descriptionMembralin
EggNOG OGsKOG2092@1,KOG2092@2759
EggNOG free text desc.positive regulation of ERAD pathway
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0006950,GO:0008150,GO:0009893,GO:0009894,GO:0009896,GO:0009987,GO:0010604,GO:0010941,GO:0012505,GO:0019222,GO:0030162,GO:0031323,GO:0031325,GO:0031329,GO:0031331,GO:0032268,GO:0032270,GO:0033554,GO:0034976,GO:0042176,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044424,GO:0044444,GO:0044464,GO:0045732,GO:0045862,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051173,GO:0051246,GO:0051247,GO:0051716,GO:0060255,GO:0060548,GO:0061136,GO:0065007,GO:0080090,GO:0080134,GO:0080135,GO:1901214,GO:1901215,GO:1901800,GO:1903050,GO:1903052,GO:1903362,GO:1903364,GO:1904292,GO:1904294,GO:1905897,GO:1905898
Preferred nameTMEM259
Seed eggNOG ortholog2880.D8LLK2
Seed ortholog evalue7.1e-85
Seed ortholog score320.9
Taxonomic scopeEukaryota
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig1477.2786.1prot_E-siliculosus-1a_F_contig1477.2786.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig1477 27779..30304 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927986.404426-CDS-E-siliculosus-1a_F_contig1477:27778..278981622927986.404426-CDS-E-siliculosus-1a_F_contig1477:27778..27898Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1477 27779..27898 +
1696318763.9952798-CDS-E-siliculosus-1a_F_contig1477:27778..278981696318763.9952798-CDS-E-siliculosus-1a_F_contig1477:27778..27898Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1477 27779..27898 +
1622927986.4199595-CDS-E-siliculosus-1a_F_contig1477:28156..282161622927986.4199595-CDS-E-siliculosus-1a_F_contig1477:28156..28216Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1477 28157..28216 +
1696318764.0067353-CDS-E-siliculosus-1a_F_contig1477:28156..282161696318764.0067353-CDS-E-siliculosus-1a_F_contig1477:28156..28216Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1477 28157..28216 +
1622927986.4324238-CDS-E-siliculosus-1a_F_contig1477:29186..293631622927986.4324238-CDS-E-siliculosus-1a_F_contig1477:29186..29363Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1477 29187..29363 +
1696318764.0154555-CDS-E-siliculosus-1a_F_contig1477:29186..293631696318764.0154555-CDS-E-siliculosus-1a_F_contig1477:29186..29363Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1477 29187..29363 +
1622927986.4447372-CDS-E-siliculosus-1a_F_contig1477:29686..303041622927986.4447372-CDS-E-siliculosus-1a_F_contig1477:29686..30304Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1477 29687..30304 +
1696318764.0243216-CDS-E-siliculosus-1a_F_contig1477:29686..303041696318764.0243216-CDS-E-siliculosus-1a_F_contig1477:29686..30304Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1477 29687..30304 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig1477.2786.1

>prot_E-siliculosus-1a_F_contig1477.2786.1 ID=prot_E-siliculosus-1a_F_contig1477.2786.1|Name=mRNA_E-siliculosus-1a_F_contig1477.2786.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=325bp
AGSLRPVQEPEEEASVSALSHKGSCYDFTWPASLSREMEQVFFRLHVRFV
TTVTRLLSPKTRRLLEYVTLANAFVLVTSLAWLHTRFVNPKGRQGSAVSC
LPAALEKAGVDPAQLHVLQIHIRQPPDDWGGGGGGGRRKNLMSFPGGSSR
IDDSSSSGTNAEQQVSDSRRHSGGATVDAGLEISGLGGGESGDGSATCTS
AATACTGGGEEEGAAGAMRSESSSSSGDGGGLLPPRLDSPPQSPEIFYSM
KLVTGKDAVGTLSGPGTLGIERCVVKGGDGVDGNNPAGGGGGGGGICGAV
VEEKRPTLVQTLMDAFSWGGRKGET
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mRNA from alignment at E-siliculosus-1a_F_contig1477:27779..30305+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig1477.2786.1 ID=mRNA_E-siliculosus-1a_F_contig1477.2786.1|Name=mRNA_E-siliculosus-1a_F_contig1477.2786.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=2527bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1477:27779..30305+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
GCAGGCAGCTTAAGGCCTGTTCAGGAACCGGAGGAAGAGGCCTCCGTCAG
CGCCTTGTCACACAAGGGCAGCTGCTACGATTTTACTTGGCCGGCATCCT
TGTCGCGAGAGATGGAGCAGGTGAACGGTACAATAAACTGCTCTTGAAAC
CTGGTATCATCCATGCTGTTATGTTGATGCTGGGGTATCCACCGCTGTGT
GCCGGATGATGCTTACCCGACAAACTGCAGTGTTTGTTCACTACTGCCTC
CGCTTTAATGCTCTCGGTGCGATTTTCTCGCTCTCTCGGTCTTTTTATGT
GGGCACCTGTTCCTCGGTCCCAAGGACTGTGACGTTTTTGAGTCGTGGTG
GTGTTTGTTTGTTCCCCTGCCCGCCAAGGTGTTCTTCCGGTTACACGTCC
GGTTTGTCACGACCGTCACAAGACTGCTCTCGCCGAAGGTAAGACTAGAC
TCACTCTCCGTGCAGGAGATATACCGTTAACAGAGAACAATCACTTGTTG
ACCGTAGGGGCGATGGCGGCCGTCGTCTCAACAGAACTGCTGTTGGTATC
ACGGGGTACTGCAGCAGCCGTAGTTACGCCGCAGCGTGTCGGCTTACATC
AAGTTGCCCTTCATGCGCTACTGCCGCTGCTGTGCTGCTGTGTGGGTGTC
TGGAATATATCGGCTCCGGCTAGGTGCTTCAAGTGCTATCATACATTGAC
GAGTTGACGTTTGCATCTCCGGTAGATCTTGTCCACGACAACATGCGTGT
TTTTCCCCCCTTTGTCCCTAAATTAGCGCGGCCTTGTATTACATCTGCGT
GGTTCACCGACCGCTTCCCTTCCAAGACGACAAGTATACATGGATACGAT
GAGGAACGCACTTTTAACGAAAGGATGGTACATGTATACGGCCGTCGTTC
AATTCGAACCGATGACGTGTCTGAATCCAGTGCACTTGCAACTCTTGCAA
ACAAAGTCGGCATCGTGCGAACAGAGTCGGCGGCGATGAGGCGGGACCAT
CACCTACATGTACATGCGCAGCTTCCGAGTCCCCACGCCATGTCGCAACT
CCCGCATCCGTTTCTGGGATTAATCAGGGGACTCGCCAGCGTGGTATATC
TACATCTGATGATATCTACTTCCTACCCCAGTTGGTTTTCAGGAAGGCTT
GCGTTAGGGATCACAAGGCTCCAGAGAGTGTGGTGCATCTGCACGAATCA
GAAACAACAGCCGTTGCAACGGTGCCCATATTTTTCTACCGCTGCCTGTC
GCTCAGCGGTTCTCCCACTTCTCCGCGAGTTCCCGCCACTTCAACATTCT
CGCGCTCCCATTTTTCTTTCACTTCAACTCTCTCCTGTCCGCATCTACAA
CACCCGCGCGCATGGTTTCGCCGCCTGATTTTGGTGGTCAAACCTGTCGT
TCTGGCAGACTCGGCGCCTGCTAGAGTACGTCACTCTCGCGAACGCTTTC
GTGCTGGTGACGTCGCTGGCTTGGCTTCACACGCGCTTCGTCAACCCGAA
AGGAAGGCAAGGGTCGGCCGTTTCGTGCCTTCCCGCCGCATTGGAGAAGG
CCGGAGTCGACCCTGCGCAGCTCCATGTTTTACAGGTCTGGTTGACCTTT
CTGTCTCTCTGTATGTGAACGACGCTTCCCTGAGGTATTTACTACCTAGC
TTCGTCAGGACAGAGAGAGCGAGATGGGCATACACCGCCGTTGGATTGAT
ACCGGAGCAAGTTTCTTAGCTCCAGGTGTAGAGCGTATTGAGCCCAACTG
CACTACTTTAACACAAGACACAGCATGAATATTTGGTGCGCTACATGTTG
CCGGTCAACGCACCCTTGTGCCTATTAGTCTATTAGTCTCAAGGGACGGT
ACTATTTCCGTGTGACGCTTGACCATCGGTTTCCCCGCCCAAATTTTTTT
ACACCCAGATACATATTCGACAACCACCCGATGATTGGGGTGGCGGCGGC
GGCGGCGGGCGGCGGAAGAACTTGATGTCTTTCCCCGGCGGCAGCAGTCG
AATTGACGACAGCAGCAGTAGTGGCACTAACGCCGAACAGCAGGTGTCCG
ACTCGCGTAGACATAGCGGTGGCGCGACCGTGGATGCTGGTCTGGAGATC
TCAGGCTTGGGCGGCGGCGAAAGTGGTGACGGTAGCGCTACTTGCACAAG
CGCCGCTACCGCTTGCACCGGCGGCGGCGAAGAAGAGGGTGCCGCCGGCG
CCATGAGGAGCGAAAGCAGCAGCAGCAGCGGTGATGGTGGTGGGCTGCTG
CCCCCGAGGTTGGACTCGCCGCCCCAGTCTCCGGAGATCTTCTACAGCAT
GAAGCTCGTCACGGGCAAGGACGCCGTGGGCACGCTGTCAGGACCGGGCA
CCCTGGGCATCGAGAGGTGCGTCGTGAAGGGAGGGGATGGCGTGGACGGG
AACAACCCCGCCGGGGGTGGCGGCGGCGGCGGCGGGATTTGCGGCGCGGT
GGTGGAAGAGAAGCGCCCGACGCTGGTGCAGACGCTGATGGACGCGTTCT
CCTGGGGGGGAAGGAAGGGAGAGACGG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig1477:27779..30305+

>mRNA_E-siliculosus-1a_F_contig1477.2786.1 ID=mRNA_E-siliculosus-1a_F_contig1477.2786.1|Name=mRNA_E-siliculosus-1a_F_contig1477.2786.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=1950bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1477:27779..30305+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
GCAGGCAGCTTAAGGCCTGTTCAGGAACCGGAGGAAGAGGCCTCCGTCAG
CGCCTTGTCACACAAGGGCAGCTGCTACGATTTTACTTGGCCGGCATCCT
TGTCGCGAGAGATGGAGCAGGCAGGCAGCTTAAGGCCTGTTCAGGAACCG
GAGGAAGAGGCCTCCGTCAGCGCCTTGTCACACAAGGGCAGCTGCTACGA
TTTTACTTGGCCGGCATCCTTGTCGCGAGAGATGGAGCAGGTGTTCTTCC
GGTTACACGTCCGGTTTGTCACGACCGTCACAAGACTGCTCTCGCCGAAG
GTGTTCTTCCGGTTACACGTCCGGTTTGTCACGACCGTCACAAGACTGCT
CTCGCCGAAGACTCGGCGCCTGCTAGAGTACGTCACTCTCGCGAACGCTT
TCGTGCTGGTGACGTCGCTGGCTTGGCTTCACACGCGCTTCGTCAACCCG
AAAGGAAGGCAAGGGTCGGCCGTTTCGTGCCTTCCCGCCGCATTGGAGAA
GGCCGGAGTCGACCCTGCGCAGCTCCATGTTTTACAGACTCGGCGCCTGC
TAGAGTACGTCACTCTCGCGAACGCTTTCGTGCTGGTGACGTCGCTGGCT
TGGCTTCACACGCGCTTCGTCAACCCGAAAGGAAGGCAAGGGTCGGCCGT
TTCGTGCCTTCCCGCCGCATTGGAGAAGGCCGGAGTCGACCCTGCGCAGC
TCCATGTTTTACAGATACATATTCGACAACCACCCGATGATTGGGGTGGC
GGCGGCGGCGGCGGGCGGCGGAAGAACTTGATGTCTTTCCCCGGCGGCAG
CAGTCGAATTGACGACAGCAGCAGTAGTGGCACTAACGCCGAACAGCAGG
TGTCCGACTCGCGTAGACATAGCGGTGGCGCGACCGTGGATGCTGGTCTG
GAGATCTCAGGCTTGGGCGGCGGCGAAAGTGGTGACGGTAGCGCTACTTG
CACAAGCGCCGCTACCGCTTGCACCGGCGGCGGCGAAGAAGAGGGTGCCG
CCGGCGCCATGAGGAGCGAAAGCAGCAGCAGCAGCGGTGATGGTGGTGGG
CTGCTGCCCCCGAGGTTGGACTCGCCGCCCCAGTCTCCGGAGATCTTCTA
CAGCATGAAGCTCGTCACGGGCAAGGACGCCGTGGGCACGCTGTCAGGAC
CGGGCACCCTGGGCATCGAGAGGTGCGTCGTGAAGGGAGGGGATGGCGTG
GACGGGAACAACCCCGCCGGGGGTGGCGGCGGCGGCGGCGGGATTTGCGG
CGCGGTGGTGGAAGAGAAGCGCCCGACGCTGGTGCAGACGCTGATGGACG
CGTTCTCCTGGGGGGGAAGGAAGGGAGAGACGATACATATTCGACAACCA
CCCGATGATTGGGGTGGCGGCGGCGGCGGCGGGCGGCGGAAGAACTTGAT
GTCTTTCCCCGGCGGCAGCAGTCGAATTGACGACAGCAGCAGTAGTGGCA
CTAACGCCGAACAGCAGGTGTCCGACTCGCGTAGACATAGCGGTGGCGCG
ACCGTGGATGCTGGTCTGGAGATCTCAGGCTTGGGCGGCGGCGAAAGTGG
TGACGGTAGCGCTACTTGCACAAGCGCCGCTACCGCTTGCACCGGCGGCG
GCGAAGAAGAGGGTGCCGCCGGCGCCATGAGGAGCGAAAGCAGCAGCAGC
AGCGGTGATGGTGGTGGGCTGCTGCCCCCGAGGTTGGACTCGCCGCCCCA
GTCTCCGGAGATCTTCTACAGCATGAAGCTCGTCACGGGCAAGGACGCCG
TGGGCACGCTGTCAGGACCGGGCACCCTGGGCATCGAGAGGTGCGTCGTG
AAGGGAGGGGATGGCGTGGACGGGAACAACCCCGCCGGGGGTGGCGGCGG
CGGCGGCGGGATTTGCGGCGCGGTGGTGGAAGAGAAGCGCCCGACGCTGG
TGCAGACGCTGATGGACGCGTTCTCCTGGGGGGGAAGGAAGGGAGAGACG
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