mRNA_E-siliculosus-1a_F_contig1305.1862.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig1305.1862.1
Unique NamemRNA_E-siliculosus-1a_F_contig1305.1862.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1862.1 vs. uniprot
Match: A0A6H5L9Y2_9PHAE (DNA-directed RNA polymerase subunit n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L9Y2_9PHAE)

HSP 1 Score: 233 bits (593), Expect = 1.470e-68
Identity = 121/127 (95.28%), Postives = 121/127 (95.28%), Query Frame = 1 
Query:    1 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLEERAAGADEAEGQEVAKR------YVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE 363
            VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLEERAAGADEAEGQEVAKR      YVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE
Sbjct: 1117 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLEERAAGADEAEGQEVAKRVVDILEYVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE 1243
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1862.1 vs. uniprot
Match: D8LRY3_ECTSI (DNA-directed RNA polymerase subunit n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LRY3_ECTSI)

HSP 1 Score: 233 bits (593), Expect = 1.490e-68
Identity = 121/127 (95.28%), Postives = 121/127 (95.28%), Query Frame = 1 
Query:    1 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLEERAAGADEAEGQEVAKR------YVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE 363
            VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLEERAAGADEAEGQEVAKR      YVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE
Sbjct: 1116 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLEERAAGADEAEGQEVAKRVVDILEYVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE 1242
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1862.1 vs. uniprot
Match: A0A836CHC4_9STRA (DNA-directed RNA polymerase subunit n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CHC4_9STRA)

HSP 1 Score: 166 bits (421), Expect = 2.180e-45
Identity = 87/125 (69.60%), Postives = 96/125 (76.80%), Query Frame = 1 
Query:    1 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLEERAAGADEAEGQEVAKR------YVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQ 357
            V  GEMCGVLAAQSIGEPITQMTLNTFH+AGVSAKNVTLGVPRVKEIINVA N RTPSLTIYL       D  + QE AKR      Y+TL D+T STQIYYDPDPR T+++ED E +E   DI+
Sbjct: 1108 VSAGEMCGVLAAQSIGEPITQMTLNTFHYAGVSAKNVTLGVPRVKEIINVAANPRTPSLTIYL-----SPDSEQDQESAKRVVDMLEYITLADVTVSTQIYYDPDPRDTIIEEDKECVEAFLDIE 1227
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1862.1 vs. uniprot
Match: W1Q7J6_OGAPD (DNA-directed RNA polymerase subunit n=9 Tax=Ogataea TaxID=461281 RepID=W1Q7J6_OGAPD)

HSP 1 Score: 161 bits (408), Expect = 1.230e-43
Identity = 79/122 (64.75%), Postives = 103/122 (84.43%), Query Frame = 1 
Query:    1 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYL-EERAAGADEAEGQEVAKRYVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE 363
            V PGEM GV+AAQSIGEP TQMTLNTFH+AGVS+KNVTLGVPR+KEI+NVAKNV+TP+LT+YL EE A+  ++A+  + A  + TL ++T ST+IYYDPDPRSTVV++D++ +E +F I +E
Sbjct: 1019 VNPGEMVGVIAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNVKTPALTVYLKEEYASDIEKAKSIQSAIEHTTLKNVTASTEIYYDPDPRSTVVEDDIDTVEAYFAIPDE 1140
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1862.1 vs. uniprot
Match: A0A1E4T1L4_9ASCO (DNA-directed RNA polymerase subunit n=1 Tax=[Candida] arabinofermentans NRRL YB-2248 TaxID=983967 RepID=A0A1E4T1L4_9ASCO)

HSP 1 Score: 160 bits (404), Expect = 4.260e-43
Identity = 76/122 (62.30%), Postives = 102/122 (83.61%), Query Frame = 1 
Query:    1 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLEER-AAGADEAEGQEVAKRYVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE 363
            V PGEM GV+AAQSIGEP TQMTLNTFH+AGVS+KNVTLGVPR+KEI+NVAKNV+TP+LT+YLEE  A+  + A+  + A  + TL ++T ST+IYYDPDP+ST++++D++ +E +F I +E
Sbjct: 1058 VNPGEMVGVIAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNVKTPALTVYLEEEYASDIENAKSIQSAIEHTTLKNVTASTEIYYDPDPKSTIIEDDIDTVEAYFAIPDE 1179
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1862.1 vs. uniprot
Match: A0A448YT48_BRENA (DNA-directed RNA polymerase subunit n=2 Tax=Brettanomyces TaxID=13366 RepID=A0A448YT48_BRENA)

HSP 1 Score: 159 bits (401), Expect = 1.080e-42
Identity = 76/122 (62.30%), Postives = 101/122 (82.79%), Query Frame = 1 
Query:    1 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLEERAAG-ADEAEGQEVAKRYVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE 363
            V PGEM GV+AAQSIGEP TQMTLNTFH+AGVS+KNVTLGVPR+KEI+NVAKN++TP+LT+YLE++ A   + A+  + A  + TL ++T  T+IYYDPDP+STV++EDV+ +E +F I +E
Sbjct: 1060 VNPGEMVGVIAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPALTVYLEDKVASDIERAKSIQSAIEHTTLKNVTAVTEIYYDPDPKSTVIEEDVDTVEAYFAIPDE 1181
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1862.1 vs. uniprot
Match: A0A1E4TPY7_PACTA (DNA-directed RNA polymerase subunit n=1 Tax=Pachysolen tannophilus NRRL Y-2460 TaxID=669874 RepID=A0A1E4TPY7_PACTA)

HSP 1 Score: 159 bits (401), Expect = 1.080e-42
Identity = 78/122 (63.93%), Postives = 103/122 (84.43%), Query Frame = 1 
Query:    1 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLE-ERAAGADEAEGQEVAKRYVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE 363
            V PGEM GV+AAQSIGEP TQMTLNTFHFAGVS+KNVTLGVPR+KEI+NVAKN++TP+LT+YLE E AA  ++A+  + A  + TL ++T +T+IYYDPDPR+TV++ED++ +E +F I +E
Sbjct: 1063 VHPGEMVGVVAAQSIGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEILNVAKNIKTPALTVYLEPEVAADIEKAKVVQSAIEHATLKNVTAATEIYYDPDPRTTVIEEDLDTVEAYFAIPDE 1184
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1862.1 vs. uniprot
Match: A0A8H7GS40_9ASCO (DNA-directed RNA polymerase subunit n=2 Tax=Metschnikowia TaxID=27320 RepID=A0A8H7GS40_9ASCO)

HSP 1 Score: 158 bits (399), Expect = 1.980e-42
Identity = 78/122 (63.93%), Postives = 102/122 (83.61%), Query Frame = 1 
Query:    1 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLE-ERAAGADEAEGQEVAKRYVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE 363
            V PGEM GV+AAQSIGEP TQMTLNTFH+AGVS+KNVTLGVPR+KEI+NVAKN++TP+LT+YLE E A   ++A+  + A  + TL ++T+ST+IYYDPDPRSTV++ED + +E +F I +E
Sbjct:  997 VHPGEMVGVVAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPALTVYLEPEIAVDIEKAKVVQSAIEHTTLKNVTSSTEIYYDPDPRSTVIEEDYDTVEAYFAIPDE 1118
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1862.1 vs. uniprot
Match: A0A4P6XX01_9ASCO (DNA-directed RNA polymerase subunit n=4 Tax=Metschnikowia TaxID=27320 RepID=A0A4P6XX01_9ASCO)

HSP 1 Score: 158 bits (399), Expect = 2.010e-42
Identity = 78/122 (63.93%), Postives = 102/122 (83.61%), Query Frame = 1 
Query:    1 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLE-ERAAGADEAEGQEVAKRYVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE 363
            V PGEM GV+AAQSIGEP TQMTLNTFH+AGVS+KNVTLGVPR+KEI+NVAKN++TP+LT+YLE E A   ++A+  + A  + TL ++T+ST+IYYDPDPRSTV++ED + +E +F I +E
Sbjct: 1018 VHPGEMVGVVAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPALTVYLEPEIAVDIEKAKVVQSAIEHTTLKNVTSSTEIYYDPDPRSTVIEEDYDTVEAYFAIPDE 1139
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1862.1 vs. uniprot
Match: A0A1L2FUN6_9MYCE (DNA-directed RNA polymerase subunit (Fragment) n=1 Tax=Coremiostelium polycephalum TaxID=142831 RepID=A0A1L2FUN6_9MYCE)

HSP 1 Score: 157 bits (398), Expect = 2.730e-42
Identity = 74/122 (60.66%), Postives = 98/122 (80.33%), Query Frame = 1 
Query:    1 VGPGEMCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVRTPSLTIYLEERAA-GADEAEGQEVAKRYVTLGDITTSTQIYYDPDPRSTVVDEDVELLEGHFDIQEE 363
            V PGEM G +AAQSIGEP TQMTLNTFHFAGVS+KNVTLGVPR+KEIIN+AKNV+TPSLT+YL+   A   D+A+  +    Y TL  +T +T+IYYDPDP++T+++EDV+ +  +F++ +E
Sbjct: 1058 VHPGEMVGAIAAQSIGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINIAKNVKTPSLTVYLKPHCARDLDKAKAVQAQLEYTTLAKVTAATEIYYDPDPQNTIIEEDVDFVNSYFELPDE 1179
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig1305.1862.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5L9Y2_9PHAE1.470e-6895.28DNA-directed RNA polymerase subunit n=1 Tax=Ectoca... [more]
D8LRY3_ECTSI1.490e-6895.28DNA-directed RNA polymerase subunit n=2 Tax=Ectoca... [more]
A0A836CHC4_9STRA2.180e-4569.60DNA-directed RNA polymerase subunit n=1 Tax=Tribon... [more]
W1Q7J6_OGAPD1.230e-4364.75DNA-directed RNA polymerase subunit n=9 Tax=Ogatae... [more]
A0A1E4T1L4_9ASCO4.260e-4362.30DNA-directed RNA polymerase subunit n=1 Tax=[Candi... [more]
A0A448YT48_BRENA1.080e-4262.30DNA-directed RNA polymerase subunit n=2 Tax=Bretta... [more]
A0A1E4TPY7_PACTA1.080e-4263.93DNA-directed RNA polymerase subunit n=1 Tax=Pachys... [more]
A0A8H7GS40_9ASCO1.980e-4263.93DNA-directed RNA polymerase subunit n=2 Tax=Metsch... [more]
A0A4P6XX01_9ASCO2.010e-4263.93DNA-directed RNA polymerase subunit n=4 Tax=Metsch... [more]
A0A1L2FUN6_9MYCE2.730e-4260.66DNA-directed RNA polymerase subunit (Fragment) n=1... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig1305contigE-siliculosus-1a_F_contig1305:33563..34303 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start1
Stop0
Cds size348
Model size363
Exons2
Hectar predicted targeting categoryother localisation
BRITEbr01611,ko00000,ko00001,ko00002,ko01000,ko03021,ko03400
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
COG Functional cat.K
EC2.7.7.6
EggNOG OGsCOG0086@1,KOG0260@2759
EggNOG free text desc.DNA-directed 5'-3' RNA polymerase activity
GOsGO:0000428,GO:0000731,GO:0003674,GO:0003676,GO:0003677,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005665,GO:0005737,GO:0006139,GO:0006259,GO:0006281,GO:0006301,GO:0006351,GO:0006366,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0010467,GO:0010494,GO:0016070,GO:0016591,GO:0018130,GO:0019438,GO:0019985,GO:0030880,GO:0031974,GO:0031981,GO:0032774,GO:0032991,GO:0033554,GO:0034641,GO:0034645,GO:0034654,GO:0035770,GO:0036464,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0046483,GO:0050896,GO:0051716,GO:0055029,GO:0061695,GO:0070013,GO:0071704,GO:0071897,GO:0090304,GO:0097159,GO:0097659,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1902494,GO:1990234,GO:1990904
KEGG ModuleM00180
KEGG Pathwayko00230,ko00240,ko01100,ko03020,ko05016,ko05168,ko05169,map00230,map00240,map01100,map03020,map05016,map05168,map05169
KEGG ReactionR00435,R00441,R00442,R00443
KEGG koko:K03006
KEGG rclassRC02795
Preferred nameRPB1
Seed eggNOG ortholog2880.D8LRY3
Seed ortholog evalue1.2e-55
Seed ortholog score222.2
Taxonomic scopeEukaryota
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig1305.1862.1prot_E-siliculosus-1a_F_contig1305.1862.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig1305 33563..34288 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927827.514701-CDS-E-siliculosus-1a_F_contig1305:33562..336821622927827.514701-CDS-E-siliculosus-1a_F_contig1305:33562..33682Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1305 33563..33682 -
1696318697.145065-CDS-E-siliculosus-1a_F_contig1305:33562..336821696318697.145065-CDS-E-siliculosus-1a_F_contig1305:33562..33682Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1305 33563..33682 -
1622927827.528721-CDS-E-siliculosus-1a_F_contig1305:34060..342881622927827.528721-CDS-E-siliculosus-1a_F_contig1305:34060..34288Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1305 34061..34288 -
1696318697.1592505-CDS-E-siliculosus-1a_F_contig1305:34060..342881696318697.1592505-CDS-E-siliculosus-1a_F_contig1305:34060..34288Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1305 34061..34288 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927827.546276-UTR-E-siliculosus-1a_F_contig1305:34288..343031622927827.546276-UTR-E-siliculosus-1a_F_contig1305:34288..34303Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1305 34289..34303 -
1696318697.168618-UTR-E-siliculosus-1a_F_contig1305:34288..343031696318697.168618-UTR-E-siliculosus-1a_F_contig1305:34288..34303Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1305 34289..34303 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig1305.1862.1

>prot_E-siliculosus-1a_F_contig1305.1862.1 ID=prot_E-siliculosus-1a_F_contig1305.1862.1|Name=mRNA_E-siliculosus-1a_F_contig1305.1862.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=116bp
MCGVLAAQSIGEPITQMTLNTFHFAGVSAKNVTLGVPRVKEIINVAKNVR
TPSLTIYLEERAAGADEAEGQEVAKRYVTLGDITTSTQIYYDPDPRSTVV
DEDVELLEGHFDIQEE
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mRNA from alignment at E-siliculosus-1a_F_contig1305:33563..34303-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig1305.1862.1 ID=mRNA_E-siliculosus-1a_F_contig1305.1862.1|Name=mRNA_E-siliculosus-1a_F_contig1305.1862.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=741bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1305:33563..34303- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
GTTGGCCCCGGCGAGATGTGCGGCGTTCTGGCGGCCCAGTCGATCGGAGA
GCCCATCACCCAGATGACGCTCAACACCTTCCACTTCGCCGGTGTTAGCG
CGAAGAACGTTACGCTGGGTGTGCCTCGAGTGAAGGAGATCATCAACGTG
GCGAAGAACGTACGGACGCCGTCTTTGACGATCTACCTGGAGGAAAGGGC
GGCGGGGGCGGACGAGGCGGAGGGGCAGGAGGTCGCCAAGCGGGTGAGTT
CGTTGTGGCGTTGTGGTGAAGATAAGGTAGATGCGTGCTCGCTTTGGGCA
TCGGGGTGGAGGGTGGGGTAGTCTTGGTACGTGGGACAAAAGAGGGCGAT
ATATATAATGTAGAGGGAGAGAAGAGAGAGGAGAGACAGACTAGGTCGGC
TTTTTGGTGTGGCGAAGCGGTATCAGAGACTGTTAGCCTTGTTTGACCGC
AAAAGGTGGGAACGAAGGAGAGGCGCGGGGGGCGTCTTACCTTAGCCTCG
CGTTAGACCAGACAACCACGTCCCCCACCCTCGCAAAGACCTCCCTCTAA
TAGCAACCAATGGGCCTCCCTGCAATCAAACCGACCCAACTCGTTGCACC
CAGGTGGTGGACATCCTGGAGTACGTGACGCTGGGGGACATCACCACGTC
GACCCAGATCTACTACGACCCTGACCCTCGATCTACGGTGGTGGACGAGG
ACGTGGAGCTGCTGGAGGGGCACTTCGACATCCAGGAGGAG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig1305:33563..34303-

>mRNA_E-siliculosus-1a_F_contig1305.1862.1 ID=mRNA_E-siliculosus-1a_F_contig1305.1862.1|Name=mRNA_E-siliculosus-1a_F_contig1305.1862.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=696bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1305:33563..34303- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGTGCGGCGTTCTGGCGGCCCAGTCGATCGGAGAGCCCATCACCCAGAT
GACGCTCAACACCTTCCACTTCGCCGGTGTTAGCGCGAAGAACGTTACGC
TGGGTGTGCCTCGAGTGAAGGAGATCATCAACGTGGCGAAGAACGTACGG
ACGCCGTCTTTGACGATCTACCTGGAGGAAAGGGCGGCGGGGGCGGACGA
GGCGGAGGGGCAGGAGGTCGCCAAGCGGATGTGCGGCGTTCTGGCGGCCC
AGTCGATCGGAGAGCCCATCACCCAGATGACGCTCAACACCTTCCACTTC
GCCGGTGTTAGCGCGAAGAACGTTACGCTGGGTGTGCCTCGAGTGAAGGA
GATCATCAACGTGGCGAAGAACGTACGGACGCCGTCTTTGACGATCTACC
TGGAGGAAAGGGCGGCGGGGGCGGACGAGGCGGAGGGGCAGGAGGTCGCC
AAGCGGTACGTGACGCTGGGGGACATCACCACGTCGACCCAGATCTACTA
CGACCCTGACCCTCGATCTACGGTGGTGGACGAGGACGTGGAGCTGCTGG
AGGGGCACTTCGACATCCAGGAGGAGTACGTGACGCTGGGGGACATCACC
ACGTCGACCCAGATCTACTACGACCCTGACCCTCGATCTACGGTGGTGGA
CGAGGACGTGGAGCTGCTGGAGGGGCACTTCGACATCCAGGAGGAG
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