mRNA_E-fasciculatus_F_contig1609.4169.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig1609.4169.1
Unique NamemRNA_E-fasciculatus_F_contig1609.4169.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig1609.4169.1 vs. uniprot
Match: A0A7S2W3U1_9STRA (Probable tRNA N6-adenosine threonylcarbamoyltransferase n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2W3U1_9STRA)

HSP 1 Score: 73.6 bits (179), Expect = 4.150e-14
Identity = 32/45 (71.11%), Postives = 37/45 (82.22%), Query Frame = 1 
Query:    1 HVVALVRQVLVDASLRPSDVDVICYTKGPGMGGPLRSCAVCARTL 135
            H+V L+R  L  A L PS VD+ICYTKGPGMGGPL+SCA+CARTL
Sbjct:  129 HIVGLIRTALEQAGLEPSQVDLICYTKGPGMGGPLQSCAICARTL 173
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BLAST of mRNA_E-fasciculatus_F_contig1609.4169.1 vs. uniprot
Match: M4C1E6_HYAAE (N(6)-L-threonylcarbamoyladenine synthase n=1 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=M4C1E6_HYAAE)

HSP 1 Score: 70.5 bits (171), Expect = 1.180e-13
Identity = 32/45 (71.11%), Postives = 38/45 (84.44%), Query Frame = 1 
Query:    1 HVVALVRQVLVDASLRPSDVDVICYTKGPGMGGPLRSCAVCARTL 135
            HVVA+VR  LV+A++ P ++D ICYTKGPGMGGPLRS AVCAR L
Sbjct:   53 HVVAIVRAALVEANVSPDELDCICYTKGPGMGGPLRSAAVCARML 97
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BLAST of mRNA_E-fasciculatus_F_contig1609.4169.1 vs. uniprot
Match: A0A2D4BH80_PYTIN (Probable tRNA N6-adenosine threonylcarbamoyltransferase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BH80_PYTIN)

HSP 1 Score: 72.0 bits (175), Expect = 1.220e-13
Identity = 32/45 (71.11%), Postives = 37/45 (82.22%), Query Frame = 1 
Query:    1 HVVALVRQVLVDASLRPSDVDVICYTKGPGMGGPLRSCAVCARTL 135
            H V +VR  LV+A++ P D+DVICYTKGPGMGGPLRS AVCAR L
Sbjct:   70 HAVGIVRAALVEANISPKDIDVICYTKGPGMGGPLRSAAVCARML 114
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BLAST of mRNA_E-fasciculatus_F_contig1609.4169.1 vs. uniprot
Match: D8M1Y3_BLAHO (Probable tRNA N6-adenosine threonylcarbamoyltransferase n=1 Tax=Blastocystis hominis TaxID=12968 RepID=D8M1Y3_BLAHO)

HSP 1 Score: 70.9 bits (172), Expect = 3.100e-13
Identity = 33/45 (73.33%), Postives = 37/45 (82.22%), Query Frame = 1 
Query:    1 HVVALVRQVLVDASLRPSDVDVICYTKGPGMGGPLRSCAVCARTL 135
            HVV+LVR  L  A L PSD+D+IC+TKGPGMGGPL SCAV ARTL
Sbjct:   62 HVVSLVRHALNVAKLEPSDIDIICFTKGPGMGGPLTSCAVAARTL 106
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BLAST of mRNA_E-fasciculatus_F_contig1609.4169.1 vs. uniprot
Match: UPI0007121025 (glycoprotein endopeptidase n=1 Tax=Blastocystis sp. subtype 4 TaxID=944170 RepID=UPI0007121025)

HSP 1 Score: 70.1 bits (170), Expect = 5.890e-13
Identity = 30/45 (66.67%), Postives = 36/45 (80.00%), Query Frame = 1 
Query:    1 HVVALVRQVLVDASLRPSDVDVICYTKGPGMGGPLRSCAVCARTL 135
            HV++LVR  L  A + P D+D+IC+TKGPGMGGPL SCAV ARTL
Sbjct:   62 HVISLVRHALTTAKMEPKDIDIICFTKGPGMGGPLTSCAVAARTL 106
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BLAST of mRNA_E-fasciculatus_F_contig1609.4169.1 vs. uniprot
Match: A0A7S3HK42_9STRA (Probable tRNA N6-adenosine threonylcarbamoyltransferase n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3HK42_9STRA)

HSP 1 Score: 69.3 bits (168), Expect = 1.190e-12
Identity = 32/45 (71.11%), Postives = 37/45 (82.22%), Query Frame = 1 
Query:    1 HVVALVRQVLVDASLRPSDVDVICYTKGPGMGGPLRSCAVCARTL 135
            H+ ALVR  L +A+L  ++VD ICYTKGPGMGGPLRSCAVCAR L
Sbjct:   69 HINALVRHALQEANLSAAEVDCICYTKGPGMGGPLRSCAVCARML 113
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BLAST of mRNA_E-fasciculatus_F_contig1609.4169.1 vs. uniprot
Match: A0A6S7ZQS1_9STRA (Probable tRNA N6-adenosine threonylcarbamoyltransferase n=1 Tax=Aplanochytrium stocchinoi TaxID=215587 RepID=A0A6S7ZQS1_9STRA)

HSP 1 Score: 68.2 bits (165), Expect = 3.060e-12
Identity = 30/45 (66.67%), Postives = 36/45 (80.00%), Query Frame = 1 
Query:    1 HVVALVRQVLVDASLRPSDVDVICYTKGPGMGGPLRSCAVCARTL 135
            HVV +VR  L +A++ P  VD IC+TKGPGMGGPL SCA+CARTL
Sbjct:   71 HVVQIVRTALNEANITPEQVDCICFTKGPGMGGPLTSCAICARTL 115
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BLAST of mRNA_E-fasciculatus_F_contig1609.4169.1 vs. uniprot
Match: A0A7S0ENS7_9CRYP (N(6)-L-threonylcarbamoyladenine synthase (Fragment) n=1 Tax=Hanusia phi TaxID=3032 RepID=A0A7S0ENS7_9CRYP)

HSP 1 Score: 65.9 bits (159), Expect = 4.020e-12
Identity = 30/46 (65.22%), Postives = 38/46 (82.61%), Query Frame = 1 
Query:    1 HVVALVRQVLVDASLRPSDVDVICYTKGPGMGGPLRSCAVCARTLA 138
            +VV LV+Q + +A+++P D+D ICYTKGPGMGGPLR CAV AR LA
Sbjct:   31 YVVQLVQQAIREAAIKPEDLDCICYTKGPGMGGPLRVCAVVARMLA 76
[Close]
BLAST of mRNA_E-fasciculatus_F_contig1609.4169.1 vs. uniprot
Match: A0A7S2WC24_9STRA (N(6)-L-threonylcarbamoyladenine synthase (Fragment) n=1 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2WC24_9STRA)

HSP 1 Score: 67.0 bits (162), Expect = 7.850e-12
Identity = 27/45 (60.00%), Postives = 38/45 (84.44%), Query Frame = 1 
Query:    1 HVVALVRQVLVDASLRPSDVDVICYTKGPGMGGPLRSCAVCARTL 135
            HVV +VR+ L +A+++P ++D IC+T+GPGMG PL SCA+CARTL
Sbjct:   65 HVVGIVREALNEANIKPEEIDCICFTQGPGMGAPLNSCAMCARTL 109
[Close]
BLAST of mRNA_E-fasciculatus_F_contig1609.4169.1 vs. uniprot
Match: A0A225X119_9STRA (Probable tRNA N6-adenosine threonylcarbamoyltransferase n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225X119_9STRA)

HSP 1 Score: 66.6 bits (161), Expect = 1.230e-11
Identity = 30/45 (66.67%), Postives = 35/45 (77.78%), Query Frame = 1 
Query:    1 HVVALVRQVLVDASLRPSDVDVICYTKGPGMGGPLRSCAVCARTL 135
            HVV +VR  L +A++ P  +D ICYTKGPGMGGPLRS AVCAR L
Sbjct:   53 HVVGIVRAALAEANVSPKQLDCICYTKGPGMGGPLRSAAVCARML 97
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig1609.4169.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S2W3U1_9STRA4.150e-1471.11Probable tRNA N6-adenosine threonylcarbamoyltransf... [more]
M4C1E6_HYAAE1.180e-1371.11N(6)-L-threonylcarbamoyladenine synthase n=1 Tax=H... [more]
A0A2D4BH80_PYTIN1.220e-1371.11Probable tRNA N6-adenosine threonylcarbamoyltransf... [more]
D8M1Y3_BLAHO3.100e-1373.33Probable tRNA N6-adenosine threonylcarbamoyltransf... [more]
UPI00071210255.890e-1366.67glycoprotein endopeptidase n=1 Tax=Blastocystis sp... [more]
A0A7S3HK42_9STRA1.190e-1271.11Probable tRNA N6-adenosine threonylcarbamoyltransf... [more]
A0A6S7ZQS1_9STRA3.060e-1266.67Probable tRNA N6-adenosine threonylcarbamoyltransf... [more]
A0A7S0ENS7_9CRYP4.020e-1265.22N(6)-L-threonylcarbamoyladenine synthase (Fragment... [more]
A0A7S2WC24_9STRA7.850e-1260.00N(6)-L-threonylcarbamoyladenine synthase (Fragment... [more]
A0A225X119_9STRA1.230e-1166.67Probable tRNA N6-adenosine threonylcarbamoyltransf... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig1609contigE-fasciculatus_F_contig1609:16824..16961 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score70.9
Seed ortholog evalue1.8e-10
Seed eggNOG ortholog272952.HpaP805769
Preferred nameKAE1
KEGG rclassRC00070,RC00416
KEGG koko:K01409,ko:K09487
KEGG ReactionR10648
KEGG Pathwayko04141,ko04151,ko04626,ko04657,ko04915,ko04918,ko05200,ko05215,ko05418,map04141,map04151,map04626,map04657,map04915,map04918,map05200,map05215,map05418
GOsGO:0000408,GO:0000722,GO:0000723,GO:0002949,GO:0003674,GO:0003676,GO:0003677,GO:0003682,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005886,GO:0006139,GO:0006259,GO:0006310,GO:0006312,GO:0006355,GO:0006357,GO:0006396,GO:0006399,GO:0006400,GO:0006725,GO:0006807,GO:0006996,GO:0008033,GO:0008150,GO:0008152,GO:0009451,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010467,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010628,GO:0016020,GO:0016043,GO:0016070,GO:0016604,GO:0016607,GO:0019219,GO:0019222,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031490,GO:0031974,GO:0031981,GO:0032200,GO:0032991,GO:0034470,GO:0034641,GO:0034660,GO:0042592,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044451,GO:0044464,GO:0045893,GO:0045935,GO:0045944,GO:0046483,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0051171,GO:0051173,GO:0051252,GO:0051254,GO:0051276,GO:0060249,GO:0060255,GO:0065007,GO:0065008,GO:0070013,GO:0070525,GO:0071704,GO:0071840,GO:0071944,GO:0080090,GO:0090304,GO:0097159,GO:1901360,GO:1901363,GO:1902680,GO:1903506,GO:1903508,GO:2000112,GO:2001141
EggNOG free text desc.Component of the EKC KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity
EggNOG OGs3QCZY@4776,COG0533@1,KOG2708@2759
EC2.3.1.234
COG Functional cat.O
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03016,ko03110
Hectar predicted targeting categoryno signal peptide or anchor
Exons1
Model size138
Cds size138
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928076.200278-CDS-E-fasciculatus_F_contig1609:16823..169611622928076.200278-CDS-E-fasciculatus_F_contig1609:16823..16961Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1609 16824..16961 -
1695995813.4893084-CDS-E-fasciculatus_F_contig1609:16823..169611695995813.4893084-CDS-E-fasciculatus_F_contig1609:16823..16961Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1609 16824..16961 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig1609.4169.1prot_E-fasciculatus_F_contig1609.4169.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig1609 16824..16961 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig1609.4169.1

>prot_E-fasciculatus_F_contig1609.4169.1 ID=prot_E-fasciculatus_F_contig1609.4169.1|Name=mRNA_E-fasciculatus_F_contig1609.4169.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=46bp
HVVALVRQVLVDASLRPSDVDVICYTKGPGMGGPLRSCAVCARTLA
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mRNA from alignment at E-fasciculatus_F_contig1609:16824..16961-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig1609.4169.1 ID=mRNA_E-fasciculatus_F_contig1609.4169.1|Name=mRNA_E-fasciculatus_F_contig1609.4169.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=138bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1609:16824..16961- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
CACGTCGTAGCCCTGGTCCGCCAGGTGCTCGTGGACGCCTCCCTACGCCC
GAGCGACGTGGACGTGATCTGCTACACCAAGGGCCCCGGGATGGGAGGGC
CGTTGAGGTCGTGCGCGGTTTGCGCCAGGACACTGGCT
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig1609:16824..16961-

>mRNA_E-fasciculatus_F_contig1609.4169.1 ID=mRNA_E-fasciculatus_F_contig1609.4169.1|Name=mRNA_E-fasciculatus_F_contig1609.4169.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=276bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1609:16824..16961- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
CACGTCGTAGCCCTGGTCCGCCAGGTGCTCGTGGACGCCTCCCTACGCCC
GAGCGACGTGGACGTGATCTGCTACACCAAGGGCCCCGGGATGGGAGGGC
CGTTGAGGTCGTGCGCGGTTTGCGCCAGGACACTGGCTCACGTCGTAGCC
CTGGTCCGCCAGGTGCTCGTGGACGCCTCCCTACGCCCGAGCGACGTGGA
CGTGATCTGCTACACCAAGGGCCCCGGGATGGGAGGGCCGTTGAGGTCGT
GCGCGGTTTGCGCCAGGACACTGGCT
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