mRNA_E-fasciculatus_F_contig15794.3984.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig15794.3984.1
Unique NamemRNA_E-fasciculatus_F_contig15794.3984.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig15794.3984.1 vs. uniprot
Match: D8LQC0_ECTSI (DNA polymerase epsilon catalytic subunit n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQC0_ECTSI)

HSP 1 Score: 122 bits (305), Expect = 2.580e-30
Identity = 84/89 (94.38%), Postives = 84/89 (94.38%), Query Frame = 1 
Query:    1 SRYQRQQSKXXXXXXXXXXXXXXXXXXXXLXXXGRKESAVKAALREQGDELDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGL 267
            SRYQRQQSKXXXXXXXXXXXXXXXXX   L XXGRKESAVKAALREQGD LDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGL
Sbjct:   29 SRYQRQQSKXXXXXXXXXXXXXXXXXAR-LSXXGRKESAVKAALREQGDALDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGL 116
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BLAST of mRNA_E-fasciculatus_F_contig15794.3984.1 vs. uniprot
Match: D7FPA2_ECTSI (DNA polymerase epsilon catalytic subunit n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FPA2_ECTSI)

HSP 1 Score: 75.9 bits (185), Expect = 1.180e-15
Identity = 33/33 (100.00%), Postives = 33/33 (100.00%), Query Frame = 1 
Query:  169 YDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGL 267
            YDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGL
Sbjct:   33 YDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGL 65
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BLAST of mRNA_E-fasciculatus_F_contig15794.3984.1 vs. uniprot
Match: W7TVX1_9STRA (DNA-directed DNA polymerase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TVX1_9STRA)

HSP 1 Score: 67.8 bits (164), Expect = 2.990e-11
Identity = 28/55 (50.91%), Postives = 38/55 (69.09%), Query Frame = 1 
Query:  103 RKESAVKAALREQGDELDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGL 267
            +K SA +   RE GDE D RFGY R+ EG PRLGW+ +M  +++PD  G E+SG+
Sbjct:   47 QKASASRYRAREAGDEFDIRFGYSRFEEGSPRLGWLLDMNASTVPDEEGRERSGM 101
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BLAST of mRNA_E-fasciculatus_F_contig15794.3984.1 vs. uniprot
Match: A0A2R5GVQ4_9STRA (DNA-directed DNA polymerase n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GVQ4_9STRA)

HSP 1 Score: 52.4 bits (124), Expect = 7.920e-6
Identity = 18/36 (50.00%), Postives = 29/36 (80.56%), Query Frame = 1 
Query:  127 ALREQGDELDKRFGYDRYSEGPPRLGWIFNMLPTSI 234
            ALREQ + +D+ +G+D ++EGP R+GW+ NMLP ++
Sbjct:   41 ALREQSERVDEMYGFDDFTEGPARVGWLLNMLPCTV 76
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BLAST of mRNA_E-fasciculatus_F_contig15794.3984.1 vs. uniprot
Match: A0A8H7RZK0_9FUNG (DNA polymerase epsilon catalytic subunit n=1 Tax=Mucor circinatus TaxID=2054153 RepID=A0A8H7RZK0_9FUNG)

HSP 1 Score: 52.0 bits (123), Expect = 1.080e-5
Identity = 19/32 (59.38%), Postives = 26/32 (81.25%), Query Frame = 1 
Query:  145 DELDKRFGYDRYSEGPPRLGWIFNMLPTSIPD 240
            DEL++RFG++RY+ GP RLGW+ NM  T +PD
Sbjct:   60 DELEERFGFERYNSGPERLGWLINMHATLVPD 91
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BLAST of mRNA_E-fasciculatus_F_contig15794.3984.1 vs. uniprot
Match: A0A1X2GTF1_9FUNG (DNA polymerase epsilon catalytic subunit n=1 Tax=Hesseltinella vesiculosa TaxID=101127 RepID=A0A1X2GTF1_9FUNG)

HSP 1 Score: 52.0 bits (123), Expect = 1.080e-5
Identity = 21/32 (65.62%), Postives = 24/32 (75.00%), Query Frame = 1 
Query:  145 DELDKRFGYDRYSEGPPRLGWIFNMLPTSIPD 240
            DE+D RFG+DRY EGP RLGW+ NM  T I D
Sbjct:   59 DEIDTRFGFDRYEEGPERLGWLMNMHATLIMD 90
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BLAST of mRNA_E-fasciculatus_F_contig15794.3984.1 vs. uniprot
Match: A0A812DY64_SEPPH (DNA polymerase epsilon catalytic subunit n=1 Tax=Sepia pharaonis TaxID=158019 RepID=A0A812DY64_SEPPH)

HSP 1 Score: 50.8 bits (120), Expect = 2.710e-5
Identity = 22/48 (45.83%), Postives = 31/48 (64.58%), Query Frame = 1 
Query:  100 GRKESAVKAALR-EQGDELDKRFGYDRYSEGPPRLGWIFNMLPTSIPD 240
            GR++S+ K   R +Q DE+D RFGY+RY +   + GW+ NM P  I D
Sbjct:   19 GREDSSEKRLQRSQQNDEIDSRFGYERYKDPSEKTGWLINMHPADILD 66
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BLAST of mRNA_E-fasciculatus_F_contig15794.3984.1 vs. uniprot
Match: A0A196SQL8_BLAHN (DNA polymerase epsilon catalytic subunit n=1 Tax=Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII) TaxID=478820 RepID=A0A196SQL8_BLAHN)

HSP 1 Score: 50.4 bits (119), Expect = 3.770e-5
Identity = 22/55 (40.00%), Postives = 37/55 (67.27%), Query Frame = 1 
Query:  103 RKESAVKAALREQGDELDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGL 267
            +K S++ AA   + + LD++FGY+ YSEGP RLGW+ N++ T +      +++GL
Sbjct:   26 QKGSSITAA---KNNALDEKFGYETYSEGPKRLGWLLNLVNTVVSKEGVPDQAGL 77
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BLAST of mRNA_E-fasciculatus_F_contig15794.3984.1 vs. uniprot
Match: A0A367JQD7_RHIAZ (DNA polymerase epsilon catalytic subunit n=5 Tax=Rhizopus TaxID=4842 RepID=A0A367JQD7_RHIAZ)

HSP 1 Score: 50.4 bits (119), Expect = 3.770e-5
Identity = 17/33 (51.52%), Postives = 27/33 (81.82%), Query Frame = 1 
Query:  145 DELDKRFGYDRYSEGPPRLGWIFNMLPTSIPDA 243
            ++++ RFG++RY EGP RLGW+ NM  T++PD+
Sbjct:   56 EDIEYRFGFERYQEGPERLGWLLNMHTTTVPDS 88
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BLAST of mRNA_E-fasciculatus_F_contig15794.3984.1 vs. uniprot
Match: UPI00065BC548 (DNA polymerase epsilon catalytic subunit A n=1 Tax=Aplysia californica TaxID=6500 RepID=UPI00065BC548)

HSP 1 Score: 50.1 bits (118), Expect = 5.110e-5
Identity = 22/50 (44.00%), Postives = 30/50 (60.00%), Query Frame = 1 
Query:  100 GRKESAVKAALREQ--GDELDKRFGYDRYSEGPPRLGWIFNMLPTSIPDA 243
            G +E + +  LR     DE+D RFGY+RY +   + GW+ NM PT I DA
Sbjct:   23 GAREDSTEKRLRRAVLNDEVDARFGYERYKDAAEKTGWLINMHPTDILDA 72
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig15794.3984.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 14
Match NameE-valueIdentityDescription
D8LQC0_ECTSI2.580e-3094.38DNA polymerase epsilon catalytic subunit n=1 Tax=E... [more]
D7FPA2_ECTSI1.180e-15100.00DNA polymerase epsilon catalytic subunit n=1 Tax=E... [more]
W7TVX1_9STRA2.990e-1150.91DNA-directed DNA polymerase n=2 Tax=Monodopsidacea... [more]
A0A2R5GVQ4_9STRA7.920e-650.00DNA-directed DNA polymerase n=1 Tax=Hondaea fermen... [more]
A0A8H7RZK0_9FUNG1.080e-559.38DNA polymerase epsilon catalytic subunit n=1 Tax=M... [more]
A0A1X2GTF1_9FUNG1.080e-565.63DNA polymerase epsilon catalytic subunit n=1 Tax=H... [more]
A0A812DY64_SEPPH2.710e-545.83DNA polymerase epsilon catalytic subunit n=1 Tax=S... [more]
A0A196SQL8_BLAHN3.770e-540.00DNA polymerase epsilon catalytic subunit n=1 Tax=B... [more]
A0A367JQD7_RHIAZ3.770e-551.52DNA polymerase epsilon catalytic subunit n=5 Tax=R... [more]
UPI00065BC5485.110e-544.00DNA polymerase epsilon catalytic subunit A n=1 Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig15794contigE-fasciculatus_F_contig15794:1112..2929 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score124.4
Seed ortholog evalue2.7e-26
Seed eggNOG ortholog2880.D8LQC0
Preferred namePOLE1
KEGG rclassRC02795
KEGG koko:K02324
KEGG ReactionR00375,R00376,R00377,R00378
KEGG Pathwayko00230,ko00240,ko01100,ko03030,ko03410,ko03420,ko05166,map00230,map00240,map01100,map03030,map03410,map03420,map05166
KEGG ModuleM00263
GOsGO:0000228,GO:0000278,GO:0003674,GO:0003676,GO:0003677,GO:0003824,GO:0003887,GO:0004518,GO:0004527,GO:0004529,GO:0004536,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005657,GO:0005694,GO:0006139,GO:0006259,GO:0006260,GO:0006261,GO:0006271,GO:0006272,GO:0006281,GO:0006284,GO:0006287,GO:0006289,GO:0006297,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0007049,GO:0008150,GO:0008152,GO:0008296,GO:0008297,GO:0008310,GO:0008408,GO:0008622,GO:0009058,GO:0009059,GO:0009987,GO:0016070,GO:0016740,GO:0016772,GO:0016779,GO:0016787,GO:0016788,GO:0016796,GO:0016895,GO:0018130,GO:0019438,GO:0022616,GO:0031974,GO:0031981,GO:0032991,GO:0033554,GO:0034061,GO:0034641,GO:0034645,GO:0034654,GO:0042575,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044446,GO:0044454,GO:0044464,GO:0045004,GO:0045005,GO:0046483,GO:0050896,GO:0051716,GO:0061695,GO:0070013,GO:0071704,GO:0071897,GO:0090304,GO:0090305,GO:0097159,GO:0140097,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1902494,GO:1990234
EggNOG free text desc.DNA replication proofreading
EggNOG OGsCOG0417@1,KOG1798@2759
EC2.7.7.7
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03032,ko03400
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionDNA polymerase epsilon catalytic subunit
Ec32 orthologEc-00_003210.1
Exons2
Model size270
Cds size267
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928049.3627665-CDS-E-fasciculatus_F_contig15794:1112..12121622928049.3627665-CDS-E-fasciculatus_F_contig15794:1112..1212Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15794 1113..1212 -
1695995802.805459-CDS-E-fasciculatus_F_contig15794:1112..12121695995802.805459-CDS-E-fasciculatus_F_contig15794:1112..1212Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15794 1113..1212 -
1622928049.4020429-CDS-E-fasciculatus_F_contig15794:2762..29291622928049.4020429-CDS-E-fasciculatus_F_contig15794:2762..2929Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15794 2763..2929 -
1695995802.8169723-CDS-E-fasciculatus_F_contig15794:2762..29291695995802.8169723-CDS-E-fasciculatus_F_contig15794:2762..2929Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15794 2763..2929 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig15794.3984.1prot_E-fasciculatus_F_contig15794.3984.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig15794 1113..2929 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig15794.3984.1

>prot_E-fasciculatus_F_contig15794.3984.1 ID=prot_E-fasciculatus_F_contig15794.3984.1|Name=mRNA_E-fasciculatus_F_contig15794.3984.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=89bp
SRYQRQQSKGGGGGSGGKRSYADSSQAARLSGGGRKESAVKAALREQGDE
LDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGL
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mRNA from alignment at E-fasciculatus_F_contig15794:1112..2929-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig15794.3984.1 ID=mRNA_E-fasciculatus_F_contig15794.3984.1|Name=mRNA_E-fasciculatus_F_contig15794.3984.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=1818bp|location=Sequence derived from alignment at E-fasciculatus_F_contig15794:1112..2929- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
TCACGATACCAGCGGCAGCAGAGCAAAGGAGGAGGGGGAGGATCGGGCGG
CAAAAGAAGCTACGCGGACTCGAGCCAAGCGGCAAGGCTCAGCGGCGGCG
GCAGGAAGGAGAGCGCGGTGAAGGCTGCTCTCCGAGAGCAGGGGGATGAG
TTGGACAAGAGATTCGGGTGCGTGGCAGTAGCTTCGCAGAAGAAGCAGCT
CCTTTCGTTAGAGTGTTTGTAGTCGTGTTCGCCGGCTGTTTTCAGTGAAT
CCGCGCGTTTTCAAAAATTTGAATTTCCTCCTTGTGTCCCAGAGCAAAGG
GGACAGCTCCTGTTGTTACCCACCATGGACCATGGACCATGCGCACACAT
CACCGGCGAAGTACGCCCTTCAAGCTAACCTTGTGTGTGTGGGAGTCCTC
TACCGGCAGCAGAAGTGCCTCGTGAACACGCGCCGCTGTCGAACATGCCC
GAAATTCTCTGTCTTCTTGCCCTCAAGGCAATTGCCCTCAAGGCAAAATC
ACCCCACAACCGCATGCATGCGTAGACTTGACTTCACGGGAGTATATACC
GTAACCACACAAGATAACACACCCCGTATTAAGCTTTTTGCATTCCGTAT
AGCTCCACAACTAGGAGGGTGCCACTGCCGAAAAGGGTGGTTTTGGAANN
NNNNNNNNCTCCTGGTTGTGGGGCTATACGGAATGCAAAAAACGTAACAC
GGGGTGTGTTATCTTGTGTATTTTGTTGGTAGTCTACAGCAGCAGTAGTC
TCCTAGGTCCTGGCCTACGTGTAGGTACACCAACACCAGCCAGACAACAT
GCATATTGCACAGCAGTGCATAGGCACAAAGAACTCCGCAGGAGACCCAA
CACTAACAGTCGTAGGTGCCTAGCTACCAGAACAACACCGCTTCCGCCTC
CGCTGCTGTGTTGCTGTCAGGAGTGTTACTGTCGATAGACGCGGATAGGC
GGACACCTGCAAACAGCGGCCATGGCTTAAACTCGTATACTACGGCATTG
CTTCCCCTCTACAGATGTATAGGTACACAGCAGCAGCAGCAGCAGCAGCA
GTGCTGCGGCATCAAACCTGCGAAAGCAGTAGCAGCAGTAGACACAACAC
AAGTGATTTTGTTAGAAGAACTGACGGATTATTGAAGCGAGAGAAGGCTC
GCCGAGCATCTTCGGTTGCTGTGGACAAGACGTGATCTGTAATTCTTCAA
CCAAAAGGGGCCTTCGTCGCCACTCTTTGCCATGCCAAGACCATCACAGT
GTACTGTCGATTGTGCTGCAGTTTGTACCTGCGTGCCTACCCATGGTGGG
TGCCTCTCGCACCCGCTCATTCGAAGATTGGGGGGAGTGATTTTGACGCC
TTGGTTGTTGGTGTGGTTGTTGTTGTCCTAACACCGATAAACGTCGCCAC
CTCTATCCAGGACCTCCTCTTTCCTGCCATACTCTCCGGAGGTATGTAAA
CGAACTTTGGTGGGTACACGCCCGAACAACTATGCAACCATTTTGCAATC
TAAGGCCGAGAGACCTCGCCTGTCACGACATGTTTCAATCGAACCACGGA
GGCGCTCATTCCGTTTGACGCACGTCAGCAGCATACGAAAACCCAACCCG
TGCCTGTCGTTTCCAGCACCTTAGACTATATCAGGGCACACACGCACTGC
ATTGACCACTACCACTACTGACCACTACCGCTGCTTTTTTGTCGATTTTT
TCCCACGGTACATCCAGGTACGACCGGTACTCCGAGGGCCCGCCGAGACT
GGGGTGGATCTTCAACATGCTGCCGACGTCCATCCCGGACGCCTCCGGCA
ACGAGAAGAGCGGGCTGG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig15794:1112..2929-

>mRNA_E-fasciculatus_F_contig15794.3984.1 ID=mRNA_E-fasciculatus_F_contig15794.3984.1|Name=mRNA_E-fasciculatus_F_contig15794.3984.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=534bp|location=Sequence derived from alignment at E-fasciculatus_F_contig15794:1112..2929- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
TCACGATACCAGCGGCAGCAGAGCAAAGGAGGAGGGGGAGGATCGGGCGG
CAAAAGAAGCTACGCGGACTCGAGCCAAGCGGCAAGGCTCAGCGGCGGCG
GCAGGAAGGAGAGCGCGGTGAAGGCTGCTCTCCGAGAGCAGGGGGATGAG
TTGGACAAGAGATTCGGTCACGATACCAGCGGCAGCAGAGCAAAGGAGGA
GGGGGAGGATCGGGCGGCAAAAGAAGCTACGCGGACTCGAGCCAAGCGGC
AAGGCTCAGCGGCGGCGGCAGGAAGGAGAGCGCGGTGAAGGCTGCTCTCC
GAGAGCAGGGGGATGAGTTGGACAAGAGATTCGGGTACGACCGGTACTCC
GAGGGCCCGCCGAGACTGGGGTGGATCTTCAACATGCTGCCGACGTCCAT
CCCGGACGCCTCCGGCAACGAGAAGAGCGGGCTGGTACGACCGGTACTCC
GAGGGCCCGCCGAGACTGGGGTGGATCTTCAACATGCTGCCGACGTCCAT
CCCGGACGCCTCCGGCAACGAGAAGAGCGGGCTG
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