mRNA_E-fasciculatus_F_contig15620.3902.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig15620.3902.1
Unique NamemRNA_E-fasciculatus_F_contig15620.3902.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig15620.3902.1 vs. uniprot
Match: A0A6H5L587_9PHAE (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5L587_9PHAE)

HSP 1 Score: 326 bits (835), Expect = 3.070e-101
Identity = 178/219 (81.28%), Postives = 187/219 (85.39%), Query Frame = 1 
Query:    1 GLRNTRHSSVPERLQVVNGLGKKADEMCRHLMTHLDKEEESALPLIKHYFTGPEMGALVGKIMGKRPTELMQTILSMMINNLPEEEVRTVMGYMKHAVKDTYFEKWLASGGFQWPTNSAPNNNSSSSKGHHGLKTNITKRAGGGAVTKRIGGNYCSGGAAWSGAGGSIHRIGCAAAGGSTASSCSVGVGGGSCGSSPFHQAVSRSSGT----ESTPGFA 645
            GLRNTRHSSV ERL+VVNGLGKKA+EMCRHLMTHLDKEEESALPLIKHYFTGPEMGALVGKIMGKRPTELMQTILSMMINNLPEEEVRTVMGYMKHAVKDTYFEKWLASGGFQWPTNSAP+N+ S     H LK ++ K AGGGAVTKRIGG+   GGAAW+GA G +HRIGCAA G STAS   VGV GGSCG SPFHQA SRSSG      S  GFA
Sbjct:  340 GLRNTRHSSVTERLEVVNGLGKKAEEMCRHLMTHLDKEEESALPLIKHYFTGPEMGALVGKIMGKRPTELMQTILSMMINNLPEEEVRTVMGYMKHAVKDTYFEKWLASGGFQWPTNSAPSNSIS----RHSLKNSVAKMAGGGAVTKRIGGSG-GGGAAWAGARGVVHRIGCAATGASTASCSGVGVDGGSCGHSPFHQAASRSSGALSADSSASGFA 553
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BLAST of mRNA_E-fasciculatus_F_contig15620.3902.1 vs. uniprot
Match: A0A7S2BX65_9STRA (Hypothetical protein (Fragment) n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2BX65_9STRA)

HSP 1 Score: 97.4 bits (241), Expect = 3.220e-22
Identity = 50/100 (50.00%), Postives = 63/100 (63.00%), Query Frame = 1 
Query:   31 PERLQVVNGLGKKADEMCRHLMTHLDKEEESALPLIKHYFTGPEMGALVGKIMGKRPTELMQTILSMMINNLPEEEVRTVMGYMKHAVKDTYFEKWLASG 330
            P RL+++  L     E   H   HL+KEE SALPLI+  FT  EM  LVG IMG+RP ELM +I++MM+ NL  EE   ++  M+ AV  TYFEKWL  G
Sbjct:   44 PRRLELLAKLETHTSEAANHFYVHLEKEENSALPLIQKVFTNEEMQQLVGDIMGRRPAELMTSIVTMMLRNLDHEESSVMLCNMQQAVAGTYFEKWLGQG 143
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BLAST of mRNA_E-fasciculatus_F_contig15620.3902.1 vs. uniprot
Match: A0A7S2RAI9_9STRA (Hypothetical protein (Fragment) n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RAI9_9STRA)

HSP 1 Score: 100 bits (250), Expect = 2.880e-21
Identity = 52/102 (50.98%), Postives = 63/102 (61.76%), Query Frame = 1 
Query:   37 RLQVVNGLGKKADEMCRHLMTHLDKEEESALPLIKHYFTGPEMGALVGKIMGKRPTELMQTILSMMINNLPEEEVRTVMGYMKHAVKDTYFEKWLASGGFQW 342
            R  +V+ L         H + HL KEE  A+PLI+  FT PEM  LVG IMG+RP ELMQ IL+MM+ NL  E   T++  MK AV +TYFEKWL   GF W
Sbjct:  177 RRALVDDLENVTSRAVDHFINHLAKEESGAMPLIQKLFTQPEMKQLVGDIMGRRPAELMQHILAMMLRNLDHEASETMLSSMKQAVTNTYFEKWLEGTGFDW 278
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BLAST of mRNA_E-fasciculatus_F_contig15620.3902.1 vs. uniprot
Match: A0A7S1XWB5_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1XWB5_9STRA)

HSP 1 Score: 97.4 bits (241), Expect = 8.330e-20
Identity = 50/116 (43.10%), Postives = 75/116 (64.66%), Query Frame = 1 
Query:    4 LRNTRHSSVPERLQVVNGLGKKADEMCRHLMTHLDKEEESALPLIKHYFTGPEMGALVGKIMGKRPTELMQTILSMMINNLPEEEVRTVMGYMKHAVKDTYFEKWLASGGFQWPTN 351
            LR     S PE   +V  LG+ A  +  +L  HLDKEE+ A+PL+K   +  E+ ALVG+IMGKR T +M  +L M++ NLP E+ R ++ +++H+ + T+FE+WLA GGF +  N
Sbjct:  152 LRRLGGQSSPEWGDLVLCLGRTAAAIVENLNEHLDKEEKGAMPLVKRLLSTEEIEALVGEIMGKRSTTMMTMLLRMVVRNLPPEDQRDMIKHLRHSAEGTFFERWLAHGGFVFDDN 267
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BLAST of mRNA_E-fasciculatus_F_contig15620.3902.1 vs. uniprot
Match: B8C1W4_THAPS (Uncharacterized protein (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8C1W4_THAPS)

HSP 1 Score: 92.8 bits (229), Expect = 3.070e-18
Identity = 48/95 (50.53%), Postives = 64/95 (67.37%), Query Frame = 1 
Query:   79 MCRHLMTHLDKEEESALPLIKHYFTGPEMGALVGKIMGKRPTELMQTILSMMINNLPEEEVRTVMGYMKHAVKDTYFEKWLASGGFQWPTNSAPN 363
            + RHLMTHLDKEE   +PL+  Y T  E+  LVGKIMGKR +ELM  IL+M + NL + +   ++ YMK A+  T+FE+WL  GG  W ++SA N
Sbjct:  273 LMRHLMTHLDKEETHCMPLVAQYLTKAEINDLVGKIMGKRSSELMSQILTMAVQNLNDADRDDMVRYMKQAMVGTFFERWLKMGG--WISSSALN 365
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BLAST of mRNA_E-fasciculatus_F_contig15620.3902.1 vs. uniprot
Match: A0A7S1G2H4_9STRA (Hypothetical protein (Fragment) n=1 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1G2H4_9STRA)

HSP 1 Score: 86.3 bits (212), Expect = 3.820e-16
Identity = 47/117 (40.17%), Postives = 68/117 (58.12%), Query Frame = 1 
Query:    4 LRNTRHS------SVPERLQVVNGLGKKADEMCRHLMTHLDKEEESALPLIKHYFTGPEMGALVGKIMGKRPTELMQTILSMMINNLPEEEVRTVMGYMKHAVKDTYFEKWLASGGF 336
            LR  RH+      S    L +++ L    + +  HL+ HLDKEE   +PLI  + +  E+  LVG IMGKR  E+++ IL M   NLP EE R ++ YM+ A+  TYFE+W+  GG+
Sbjct:  197 LRRLRHAVQAADVSRGSLLSIISSLLHCTEALASHLLVHLDKEETQCMPLIAKHLSPREITDLVGNIMGKRSGEVIKQILCMAAQNLPGEERRDMVRYMREAMVGTYFERWMVMGGY 313
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BLAST of mRNA_E-fasciculatus_F_contig15620.3902.1 vs. uniprot
Match: A0A7S2LPJ4_9STRA (Hypothetical protein (Fragment) n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2LPJ4_9STRA)

HSP 1 Score: 73.6 bits (179), Expect = 1.150e-12
Identity = 36/80 (45.00%), Postives = 53/80 (66.25%), Query Frame = 1 
Query:   73 DEMCRHLMTHLDKEEESALPLIKHYFTGPEMGALVGKIMGKRPTELMQTILSMMINNLPEEEVRTVMGYMKHAVKDTYFE 312
            D + +HL+ HL KEE   +PL+K + +  E+  LVGKIMGKR +++M  ILS+ + NLP  E   ++ YMK A+  T+FE
Sbjct:  108 DLLSQHLLRHLAKEESQCMPLVKKHLSEVEINDLVGKIMGKRSSDIMGQILSLAVRNLPPTEREEMVLYMKQAMIGTFFE 187
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig15620.3902.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
A0A6H5L587_9PHAE3.070e-10181.28Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A7S2BX65_9STRA3.220e-2250.00Hypothetical protein (Fragment) n=1 Tax=Florenciel... [more]
A0A7S2RAI9_9STRA2.880e-2150.98Hypothetical protein (Fragment) n=2 Tax=Rhizochrom... [more]
A0A7S1XWB5_9STRA8.330e-2043.10Hypothetical protein n=1 Tax=Phaeomonas parva TaxI... [more]
B8C1W4_THAPS3.070e-1850.53Uncharacterized protein (Fragment) n=1 Tax=Thalass... [more]
A0A7S1G2H4_9STRA3.820e-1640.17Hypothetical protein (Fragment) n=1 Tax=Corethron ... [more]
A0A7S2LPJ4_9STRA1.150e-1245.00Hypothetical protein (Fragment) n=1 Tax=Leptocylin... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig15620contigE-fasciculatus_F_contig15620:716..2977 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score317.4
Seed ortholog evalue5.2e-84
Seed eggNOG ortholog2880.D7FUX1
KEGG koko:K16276
EggNOG free text desc.zinc ion binding
EggNOG OGsKOG1940@1,KOG1940@2759
Ec32 ortholog descriptionZinc finger, RING-type
Ec32 orthologEc-26_004520.1
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04121
Hectar predicted targeting categoryother localisation
Exons4
Model size648
Cds size645
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig15620.3902.1prot_E-fasciculatus_F_contig15620.3902.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig15620 716..2975 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928036.9770591-CDS-E-fasciculatus_F_contig15620:715..8261622928036.9770591-CDS-E-fasciculatus_F_contig15620:715..826Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15620 716..826 +
1695995798.0761356-CDS-E-fasciculatus_F_contig15620:715..8261695995798.0761356-CDS-E-fasciculatus_F_contig15620:715..826Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15620 716..826 +
1622928036.9970615-CDS-E-fasciculatus_F_contig15620:1522..16271622928036.9970615-CDS-E-fasciculatus_F_contig15620:1522..1627Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15620 1523..1627 +
1695995798.0899162-CDS-E-fasciculatus_F_contig15620:1522..16271695995798.0899162-CDS-E-fasciculatus_F_contig15620:1522..1627Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15620 1523..1627 +
1622928037.0096166-CDS-E-fasciculatus_F_contig15620:2194..23981622928037.0096166-CDS-E-fasciculatus_F_contig15620:2194..2398Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15620 2195..2398 +
1695995798.0991538-CDS-E-fasciculatus_F_contig15620:2194..23981695995798.0991538-CDS-E-fasciculatus_F_contig15620:2194..2398Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15620 2195..2398 +
1622928037.0236106-CDS-E-fasciculatus_F_contig15620:2750..29751622928037.0236106-CDS-E-fasciculatus_F_contig15620:2750..2975Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15620 2751..2975 +
1695995798.1080563-CDS-E-fasciculatus_F_contig15620:2750..29751695995798.1080563-CDS-E-fasciculatus_F_contig15620:2750..2975Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15620 2751..2975 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig15620.3902.1

>prot_E-fasciculatus_F_contig15620.3902.1 ID=prot_E-fasciculatus_F_contig15620.3902.1|Name=mRNA_E-fasciculatus_F_contig15620.3902.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=215bp
GLRNTRHSSVPERLQVVNGLGKKADEMCRHLMTHLDKEEESALPLIKHYF
TGPEMGALVGKIMGKRPTELMQTILSMMINNLPEEEVRTVMGYMKHAVKD
TYFEKWLASGGFQWPTNSAPNNNSSSSKGHHGLKTNITKRAGGGAVTKRI
GGNYCSGGAAWSGAGGSIHRIGCAAAGGSTASSCSVGVGGGSCGSSPFHQ
AVSRSSGTESTPGFA
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mRNA from alignment at E-fasciculatus_F_contig15620:716..2977+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig15620.3902.1 ID=mRNA_E-fasciculatus_F_contig15620.3902.1|Name=mRNA_E-fasciculatus_F_contig15620.3902.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=2262bp|location=Sequence derived from alignment at E-fasciculatus_F_contig15620:716..2977+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GGACTGCGAAACACGCGGCACAGCAGCGTCCCCGAGCGGCTGCAGGTCGT
GAACGGTCTCGGCAAGAAGGCCGACGAGATGTGCAGGCACCTCATGACCC
ACCTCGACAAGGTGTGCGTGTGCTGCTGCTGCTGCTGCTGCTGCTGCTGC
TGCTGCTGCTGCTGGTCCGCACGACACCAGCATTGTTATTGATTTTGTTT
TGGGGGACGTGGAGGATGCTCGTGTGCCTGGGTAATAAGTCTGTCGGTCT
CATCTTTTTGGTCAACGCGGGCTGGTCGCACACGAAATTTTGGTATTTTG
ATGGCGTTAAGTTGTAAGAGGTCCCAGCTCTCTTGTTGTTGACAACGGTT
AACTAGGCGTTCGAGACGAAACGGGTTTCAGGCATGGTAGTAGTTGGCGA
ATTTCCTTCGGACATTGTCAAAGCACCGACTCCATCGGGTGTCGGACAGC
AAGTAGTTGTACACAGCCCCCATATTGCGTACTGTAGAATCGGGCTGTTG
CGTGTCCGTCGGTTCTGTAGAGTGATTCTTTGGTCGGGTTGATGAATGTC
TGGCGTAAGTCAACCGCAAGCGATGCTAACTCCTTGACAGTTGCCGCAGC
ATCACGGCACATTCAACCGCAGTGCCCGATTGCGGCTACATACCTCCTCG
TAACTGTTGTTTCGATTTCTTGGCGCTTCATACTTTTCTTTTGCTCAGAT
GTGCAGCAGCGTGCAAGTTTTCCTCTTAGCCTCGTACTTCTACATAATTT
GACACACGCCTACTATCCTTGTGACCGCGCGACGCGTTGTGTGATTCGTA
TATACAGGAGGAAGAGAGCGCCCTCCCCCTGATCAAGCACTACTTCACCG
GGCCTGAGATGGGGGCTCTGGTAGGGAAGATCATGGGCAAGCGTCCGACC
GAGCTTATGCAGGTAACAAAAACAAGAGCCATTTTTCTTTTTATGTATCA
TGTTTTTTGGGCACAACGTTATGACCACCTAGATATATATGGGGGCGTCG
CCTCCTCGACCCTTCAAAGCGTGCTTCGACGTGCACGGGGGCGAAGGATG
GAGAACGAAGTTTTTTCTTGTTACCCCTATGCTCCCCACCGCACTGTTGT
GCGCGGGAGCGTTGGGTGTTTTGTGTGTTCAAGTCATGACACAAACACAC
AGACAGAGCATAAGCTGCGGTCGATGCTATATTAAAATATAGCACACAGA
GACAACGAGCACAGCTGCCTGCGTTCTAGCTGCTATTTAGTGCCAATGTT
CCTCTTGCCCCCCAATCATCCGGGGGGCAACGCTCGCGCCTCCCCTGAAG
TGCCTACACACCTGCCTCGTGCACAAGCCACACGCTTCCTCTCTTCGTTT
TTGTTTCCGCTCACTGCGTTGAAACAATACAAAATTGCAGGCAATCTTGT
CGATGATCGTCGATAACCAACCCCCCCTCTCTCTCTTATTGTTGCCATTC
CCTGCGGTTAAAAAAAAAAAACTGCACAGACGATCTTGTCGATGATGATC
AACAACCTGCCCGAGGAGGAGGTGCGTACCGTGATGGGCTACATGAAGCA
CGCCGTCAAGGACACGTACTTCGAGAAGTGGTTGGCTTCGGGAGGGTTCC
AGTGGCCGACGAACAGCGCGCCCAACAACAACAGCAGCAGCAGCAAAGGC
CACCACGGCTTGAAGACCAACATTACCAAGCGGGTGCGTGCGTGGTGTCG
TCCCGTCTTGTTGTTGCGCGAAGTAAGCCCTGCCAATCTTTGCGCGTGAG
GGTCGGGGGAGGTTACTCATGTAAACGCATGCCGTCATCATGATATCACC
GACCTCGCATCCCTACAATGCCGGTGCGGAGCGGTATGCCCAGGTATCGC
CAGCCCAGGTTACCTGTGACTTGAGGGTCGGGCGTGTTTTTCAAGAACTG
CCGAATTGCAGCAGATTGGTGTCAGTAAACAGGACGACCGGACAAAACGG
CTAGATTTTTTTGTATGTGATGGTACCCTCTCTGCTAGACGTACGATGTG
ACGGTGCCCCCGCCTTGGCTTGATCGTGCCCGCAGGCGGGAGGAGGGGCG
GTCACCAAGAGAATCGGCGGCAACTACTGCAGCGGGGGCGCCGCGTGGTC
AGGAGCGGGGGGGAGTATCCACCGCATTGGTTGTGCCGCCGCCGGCGGTA
GCACCGCCTCTTCCTGCAGCGTCGGGGTCGGCGGCGGCAGCTGCGGCTCT
AGCCCGTTCCACCAGGCGGTCTCGCGCTCTTCAGGCACCGAAAGCACCCC
TGGCTTCGCTGC
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig15620:716..2977+

>mRNA_E-fasciculatus_F_contig15620.3902.1 ID=mRNA_E-fasciculatus_F_contig15620.3902.1|Name=mRNA_E-fasciculatus_F_contig15620.3902.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=1290bp|location=Sequence derived from alignment at E-fasciculatus_F_contig15620:716..2977+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GGACTGCGAAACACGCGGCACAGCAGCGTCCCCGAGCGGCTGCAGGTCGT
GAACGGTCTCGGCAAGAAGGCCGACGAGATGTGCAGGCACCTCATGACCC
ACCTCGACAAGGGACTGCGAAACACGCGGCACAGCAGCGTCCCCGAGCGG
CTGCAGGTCGTGAACGGTCTCGGCAAGAAGGCCGACGAGATGTGCAGGCA
CCTCATGACCCACCTCGACAAGGAGGAAGAGAGCGCCCTCCCCCTGATCA
AGCACTACTTCACCGGGCCTGAGATGGGGGCTCTGGTAGGGAAGATCATG
GGCAAGCGTCCGACCGAGCTTATGCAGGAGGAAGAGAGCGCCCTCCCCCT
GATCAAGCACTACTTCACCGGGCCTGAGATGGGGGCTCTGGTAGGGAAGA
TCATGGGCAAGCGTCCGACCGAGCTTATGCAGACGATCTTGTCGATGATG
ATCAACAACCTGCCCGAGGAGGAGGTGCGTACCGTGATGGGCTACATGAA
GCACGCCGTCAAGGACACGTACTTCGAGAAGTGGTTGGCTTCGGGAGGGT
TCCAGTGGCCGACGAACAGCGCGCCCAACAACAACAGCAGCAGCAGCAAA
GGCCACCACGGCTTGAAGACCAACATTACCAAGCGGACGATCTTGTCGAT
GATGATCAACAACCTGCCCGAGGAGGAGGTGCGTACCGTGATGGGCTACA
TGAAGCACGCCGTCAAGGACACGTACTTCGAGAAGTGGTTGGCTTCGGGA
GGGTTCCAGTGGCCGACGAACAGCGCGCCCAACAACAACAGCAGCAGCAG
CAAAGGCCACCACGGCTTGAAGACCAACATTACCAAGCGGGCGGGAGGAG
GGGCGGTCACCAAGAGAATCGGCGGCAACTACTGCAGCGGGGGCGCCGCG
TGGTCAGGAGCGGGGGGGAGTATCCACCGCATTGGTTGTGCCGCCGCCGG
CGGTAGCACCGCCTCTTCCTGCAGCGTCGGGGTCGGCGGCGGCAGCTGCG
GCTCTAGCCCGTTCCACCAGGCGGTCTCGCGCTCTTCAGGCACCGAAAGC
ACCCCTGGCTTCGCTGCGGGAGGAGGGGCGGTCACCAAGAGAATCGGCGG
CAACTACTGCAGCGGGGGCGCCGCGTGGTCAGGAGCGGGGGGGAGTATCC
ACCGCATTGGTTGTGCCGCCGCCGGCGGTAGCACCGCCTCTTCCTGCAGC
GTCGGGGTCGGCGGCGGCAGCTGCGGCTCTAGCCCGTTCCACCAGGCGGT
CTCGCGCTCTTCAGGCACCGAAAGCACCCCTGGCTTCGCT
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