mRNA_E-fasciculatus_F_contig15175.3662.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig15175.3662.1
Unique NamemRNA_E-fasciculatus_F_contig15175.3662.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig15175.3662.1 vs. uniprot
Match: D8LBY5_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBY5_ECTSI)

HSP 1 Score: 303 bits (776), Expect = 6.730e-101
Identity = 151/165 (91.52%), Postives = 155/165 (93.94%), Query Frame = 1 
Query:    1 GTKQSLLNGFLKRRKRDIVLVVLTVILLVSAWLLWPILFSLRVDGTTSMSWAAVWTPLWIADGIGLMFFVFLVSWGGIKAPAGWQGEWRDPYPMSMRVLALVKWGLLVVFQVCLVLRLDRHISWSWEEVLAPLMAWVWLRIGGELYRIVLQTKRLFVGRVVAAIK 495
            G  +SLLNGFLKRRKRDI LVVLTVILLVS  LLWPILFSLRVDGTTSMSWAAVWTPLWI DGIGLMFFVF+VSWG IKAPA WQGEWRDPYPMSMRVLALVKWGLLV+FQV LVLRLDRHISWSWEEVLAPLMAWVWLRI GELYRIVLQTKRLFVGRV+AAIK
Sbjct:   33 GGTESLLNGFLKRRKRDIALVVLTVILLVSVSLLWPILFSLRVDGTTSMSWAAVWTPLWITDGIGLMFFVFIVSWGPIKAPAEWQGEWRDPYPMSMRVLALVKWGLLVLFQVLLVLRLDRHISWSWEEVLAPLMAWVWLRIAGELYRIVLQTKRLFVGRVIAAIK 197
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BLAST of mRNA_E-fasciculatus_F_contig15175.3662.1 vs. uniprot
Match: A0A6H5JKW2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKW2_9PHAE)

HSP 1 Score: 228 bits (582), Expect = 1.410e-72
Identity = 110/117 (94.02%), Postives = 111/117 (94.87%), Query Frame = 1 
Query:  145 MSWAAVWTPLWIADGIGLMFFVFLVSWGGIKAPAGWQGEWRDPYPMSMRVLALVKWGLLVVFQVCLVLRLDRHISWSWEEVLAPLMAWVWLRIGGELYRIVLQTKRLFVGRVVAAIK 495
            MSWAAVWTPLWIAD IGLMFFVFLVSWG IKAPA WQGEWRDPYPMSMRVLALVKWGLLV+FQV LVLRLD HISWSWEEVLAPLMAWVWLRI GELYRIVLQTKRLFVGRVVAAIK
Sbjct:    1 MSWAAVWTPLWIADSIGLMFFVFLVSWGPIKAPAEWQGEWRDPYPMSMRVLALVKWGLLVLFQVLLVLRLDHHISWSWEEVLAPLMAWVWLRIIGELYRIVLQTKRLFVGRVVAAIK 117
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BLAST of mRNA_E-fasciculatus_F_contig15175.3662.1 vs. uniprot
Match: A0A6H5K446_9PHAE (J domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K446_9PHAE)

HSP 1 Score: 154 bits (390), Expect = 2.460e-41
Identity = 75/144 (52.08%), Postives = 102/144 (70.83%), Query Frame = 1 
Query:    7 KQSLLNGFLKRRKRDIVLVVLTVILLVSAWLLWPILFSLRVDGTTSMSWAAVWTPLWIADGIGLMFFVFLVSWGGIKAPAGWQGEWRDPYPMSMRVLALVKWGLLVVFQVCLVLRLDRHISWSWEEVLAPLMAWVWLRIGGELY 438
            K +++  FL+   RD + +V+ V+L V A LLWPILF  +VDG TS SW A+WTPLWI D +GL +F++L+S G IKAP G +  W DP+P+ +RVLALVKW LL +FQV L ++LD  I WS+  V +PL+AW+ L I G +Y
Sbjct:  105 KDNMIKNFLRMGTRDRLRLVVFVLLFVGAVLLWPILFCAKVDGDTSASWVALWTPLWIYDALGLWYFMYLISLGQIKAPEGMEEGWTDPFPLPLRVLALVKWTLLFLFQVFLTMQLDGDIDWSYAVVGSPLLAWMGLLIVGHVY 248
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BLAST of mRNA_E-fasciculatus_F_contig15175.3662.1 vs. uniprot
Match: A0A7S1UVB4_9STRA (Hypothetical protein n=1 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1UVB4_9STRA)

HSP 1 Score: 82.8 bits (203), Expect = 2.740e-15
Identity = 55/147 (37.41%), Postives = 74/147 (50.34%), Query Frame = 1 
Query:   94 WLLWPILFSLRVDGTTSMSWAAVWTPLWIADGIGLMFFVFLVSWGGIKAPAGWQGE-WRDPYPMSMRVLALVKWGLLVVFQVCLVLRLDRHIS-WSWEEVLAPLMAW----VWLRIGGELYRIV-LQTKRLFVGRVVAAIKVREKEL 513
            WL  PI+  L VD     +WA    PLWI D     +   ++  G I  P     E W DP PMS R+ +L ++GLLV+FQV +VLRLD  +S W W  V  PL  W    +W +      RIV ++     +G+  +   V EKEL
Sbjct:  142 WL--PIMVCLHVDDVLKATWAGTLLPLWIWDAFIFFYHSRVIMMGPIPRPDHISPEDWVDPLPMSKRIFSLARFGLLVLFQVLMVLRLDGVVSSWPWAVVFGPLYIWEFTTLWKKWPLARMRIVTVEDLETALGKPFSEFTVAEKEL 286
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BLAST of mRNA_E-fasciculatus_F_contig15175.3662.1 vs. uniprot
Match: A0A139AY67_GONPJ (DnaJ-domain-containing protein n=1 Tax=Gonapodya prolifera (strain JEL478) TaxID=1344416 RepID=A0A139AY67_GONPJ)

HSP 1 Score: 58.9 bits (141), Expect = 4.860e-7
Identity = 41/130 (31.54%), Postives = 67/130 (51.54%), Query Frame = 1 
Query:   49 DIVLVVLTVILLVSAWLLWPILFSLRVDGTTSMSWAAVWTPLWIADGIGLMFFVFLVSWGGIKAPAGWQGEWRDPYPMSMR--------------VLALVKWGLLVVFQVCLVLRLDRHISWSWEEVLAP 396
            ++++VV T+ +L    LL+PI  SL++DG  S SWA +  P+WI D +      FL++    K     +G+  +   +S R                 L+++GL++V QV LVLRL   +SW W  + +P
Sbjct:  115 NVLVVVATLFIL--TLLLFPIFLSLQIDGHISWSWAVIGIPIWIFDVLAF----FLIAGAPTKGEHLDEGDDPNHSGLSQREREQARQRAARTAKYFNLLEFGLVLVAQVFLVLRLSGTVSWVWAAIFSP 238
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BLAST of mRNA_E-fasciculatus_F_contig15175.3662.1 vs. uniprot
Match: A0A2R5H1F3_9STRA (DnaJ protein-like n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5H1F3_9STRA)

HSP 1 Score: 55.8 bits (133), Expect = 5.630e-6
Identity = 39/134 (29.10%), Postives = 66/134 (49.25%), Query Frame = 1 
Query:   40 RKRDIVLVVLTVILLVSAWLLWPILFSLRVDGTTSMS------WAAVWTPLWIADGIGLMFFVFLVSWGGI-----KAPAGWQGEWRDPYPM--SMRVLALVKWGLLVVFQVCLVLRLDRHISWSWEEVLAPLM 402
            RKRD  ++V  +  +    LL+PIL S+R D     S      WA  + PLW+ + +  + F   V    +     +     +  W +   +   +R+   +K GL+++F++ LVL+LD  + WSW  VL P+M
Sbjct:   94 RKRDRFMLVFAISFVSFILLLFPILLSIRWDALEGGSDSKPYAWAVAFIPLWVLEVLVTVIFAEQVRQEPLDKSDPEVDDDVRAMWNENERVFNRLRIEFGIKMGLVLIFEIFLVLQLDEVVDWSWYVVLLPMM 227
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BLAST of mRNA_E-fasciculatus_F_contig15175.3662.1 vs. uniprot
Match: A0A8H7ER49_9FUNG (J domain-containing protein n=1 Tax=Apophysomyces ossiformis TaxID=679940 RepID=A0A8H7ER49_9FUNG)

HSP 1 Score: 52.8 bits (125), Expect = 6.230e-5
Identity = 36/118 (30.51%), Postives = 50/118 (42.37%), Query Frame = 1 
Query:  118 SLRVDGTTSMSWAAVWTPLWIADGIGLMFFVFLVSWGGIKAPAGWQGEW----------------------RDPYPMSMRVLALVKWGLLVVFQVCLVLRLDRHISWSWEEVLAPLMA 405
            ++R+D  T  SWA VW PLWI D I   +  F      + +                              R    +  R+L LV W LL++FQV +VLRLD  ++WS   + AP  A
Sbjct:  114 TVRIDEITQWSWAVVWIPLWIIDAI-CCYAAFQHVLKAVNSKDXXXXXXXXXXXXXXXXXXXXXSEQLRATRRRVRLFRRLLYLVYWVLLLLFQVFIVLRLDDKVTWSATVIFAPYFA 230
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig15175.3662.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
D8LBY5_ECTSI6.730e-10191.52Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JKW2_9PHAE1.410e-7294.02Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5K446_9PHAE2.460e-4152.08J domain-containing protein n=2 Tax=Ectocarpus Tax... [more]
A0A7S1UVB4_9STRA2.740e-1537.41Hypothetical protein n=1 Tax=Grammatophora oceanic... [more]
A0A139AY67_GONPJ4.860e-731.54DnaJ-domain-containing protein n=1 Tax=Gonapodya p... [more]
A0A2R5H1F3_9STRA5.630e-629.10DnaJ protein-like n=1 Tax=Hondaea fermentalgiana T... [more]
A0A8H7ER49_9FUNG6.230e-530.51J domain-containing protein n=1 Tax=Apophysomyces ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig15175contigE-fasciculatus_F_contig15175:712..3077 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score161.8
Seed ortholog evalue2.9e-37
Seed eggNOG ortholog2880.D7G6F0
KEGG koko:K10752
KEGG Pathwayko04218,map04218
EggNOG free text desc.protein folding
EggNOG OGsCOG0484@1,KOG0714@2759
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03036
Hectar predicted targeting categorysignal anchor
Exons4
Model size522
Cds size522
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig15175.3662.1prot_E-fasciculatus_F_contig15175.3662.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig15175 712..3077 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927997.1043723-CDS-E-fasciculatus_F_contig15175:711..7921622927997.1043723-CDS-E-fasciculatus_F_contig15175:711..792Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15175 712..792 -
1695995782.7660012-CDS-E-fasciculatus_F_contig15175:711..7921695995782.7660012-CDS-E-fasciculatus_F_contig15175:711..792Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15175 712..792 -
1622927997.1195822-CDS-E-fasciculatus_F_contig15175:1317..14251622927997.1195822-CDS-E-fasciculatus_F_contig15175:1317..1425Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15175 1318..1425 -
1695995782.778681-CDS-E-fasciculatus_F_contig15175:1317..14251695995782.778681-CDS-E-fasciculatus_F_contig15175:1317..1425Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15175 1318..1425 -
1622927997.1354284-CDS-E-fasciculatus_F_contig15175:2184..23241622927997.1354284-CDS-E-fasciculatus_F_contig15175:2184..2324Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15175 2185..2324 -
1695995782.788442-CDS-E-fasciculatus_F_contig15175:2184..23241695995782.788442-CDS-E-fasciculatus_F_contig15175:2184..2324Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15175 2185..2324 -
1622927997.1519997-CDS-E-fasciculatus_F_contig15175:2884..30771622927997.1519997-CDS-E-fasciculatus_F_contig15175:2884..3077Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15175 2885..3077 -
1695995782.7995987-CDS-E-fasciculatus_F_contig15175:2884..30771695995782.7995987-CDS-E-fasciculatus_F_contig15175:2884..3077Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig15175 2885..3077 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig15175.3662.1

>prot_E-fasciculatus_F_contig15175.3662.1 ID=prot_E-fasciculatus_F_contig15175.3662.1|Name=mRNA_E-fasciculatus_F_contig15175.3662.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=174bp
GTKQSLLNGFLKRRKRDIVLVVLTVILLVSAWLLWPILFSLRVDGTTSMS
WAAVWTPLWIADGIGLMFFVFLVSWGGIKAPAGWQGEWRDPYPMSMRVLA
LVKWGLLVVFQVCLVLRLDRHISWSWEEVLAPLMAWVWLRIGGELYRIVL
QTKRLFVGRVVAAIKVREKELSS*
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mRNA from alignment at E-fasciculatus_F_contig15175:712..3077-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig15175.3662.1 ID=mRNA_E-fasciculatus_F_contig15175.3662.1|Name=mRNA_E-fasciculatus_F_contig15175.3662.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=2366bp|location=Sequence derived from alignment at E-fasciculatus_F_contig15175:712..3077- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GGGACCAAACAGTCTCTGCTGAACGGCTTCCTCAAGAGACGCAAGCGAGA
CATCGTGCTGGTGGTGCTCACGGTGATTCTCCTGGTCAGCGCGTGGCTGC
TGTGGCCCATCTTGTTCTCACTGAGGGTGGACGGGACGACCTCCATGTCC
TGGGCGGCCGTGTGGACCCCCCTGTGGATTGCCGATGGCATTGGTAAGAT
ACAGCAGTGCAGGACGAAAGCCTTGTTTTCTAGAAGCGTGCAAAGAAATG
TTTGGACACGAGATCCAGGATAGTCAGGATTGCATTTCGTTTATGGCTTC
CAATCACAACGCCCTTTTCCAGATCATTCCTTTTCGCGATTTATTCCTAA
ACGAATCTCACCTTCCAGTATTCCCCCACGATGGCGGTTGATGTTGCTGT
AGAGGAAGTTCATGTCACTCCAAGTTTTCGGAATGGTTGTACCTTGCGCG
CGGCAGCAAAGACGGGGAATCAACCACACCATTGGTCTTATCACAAAATG
TGTTCCTACGCCTGCGGTATGTAGGGATGGTTTATCCCGTCTCGTTGTTG
GGCACATTCGCCAGGAGTTAAAAACCTATCTGCTGCTGATTGTGCACTAT
TTCAGTGTGACTCAAGTCCAGTTTTTCGGCGCTTTCAATGCCGTCGTTCT
TTTCTTCCACGTTATATCGCGGGCTCGGCGTTTCTGCTGGCCATTGTTGG
CGCTGCCCATTTGCGCACTCCTTGACTCGTTGTTTGCTCCACCGCTACAA
CAGGGTTGATGTTCTTCGTATTTCTAGTGAGCTGGGGAGGTATCAAGGCC
CCGGCCGGGTGGCAGGGCGAGTGGAGAGACCCTTACCCGATGTCGATGAG
GGTGCTCGCGCTCGTGAAGTGGGGTTTGTTGGTTGTGTTTCAGGTATGTT
ACCTTGACCACGCCCACCGCTTTTGGCGACACGGCTGTCAGTGCTGACTC
CTTCGGCGGTCTAAGGTGCCCCCGTGTTGTAGGCTTGGCGTGATGTTCTC
TGGCCAGCGACTGGTATTCTGTAGTGCTGCGTGCACAGGAAAGTCACTCA
TTGACAGATTTTGTGCGACAGCGAGGCGTGGTAGTGGTGTGACGCGGCGA
ATGATTTGTGCTGTTTTCAACCTATTGCCTTCGGAAATCAAGGCTATCCG
ACTACTGTTGGGTCACTATACTTGCACAGGTTTGGGTTCCCCCACCTGCC
TTGGTACGGCGGTCTTGGCGAAACACGGCACTTGGCACGCGCATGGACGG
ACTTGACTTACACATGTAGCGTTGTGTGTTTTATTGTTTTTATCCGCTGC
CTTGGTGCCTTTGCTGGAGGTCCTTTGCTCTGGCGTTGAAACTGCTGATC
CATCTCCGCTCGTGTCGGCGATCAACGAACTACGTTGAGCACCTTGCACG
TCCGTGAAACGACGCGAAACTCCCACAGTGCTTTCCAGCTCAACGCAGTC
GTGACCTTCACCCATGCAAGTGTCATCAGGCGGTCGTCGTTTCTCGCTCT
GTGTTGCTGATCGTGTCTATACCACACATATATATATAGGGTCGTGTGCA
CACCCGTGCTACGTAGCTGCGTGCCGTAGCGCATTCACTCCATTGTTCCA
ACGCCGTAGCTCATCTTGAAGGCAAAACCCTCCCTGCACGCAACTAACGC
AGGTGTGCTTAGTTCTCAGGTTGGATCGCCACATCAGCTGGTCTTGGGAG
GAAGTCTTGGCGCCGCTCATGGCGTGGGTCTGGCTTCGGATTGGTGGAGA
GCTGTATAGGGTGCGTGTGGTGACTGCAATACCGGTTAAAGTTTGGTGAC
GCTGCAAATTGCGGGATCAGGTGGTGGGATGGAATAAAAGGTAGAGATAG
CAGCTGGTTTCGAGGCAGTTGGTACATATTCAGCGTCCACAGCTGTGAAA
ACCGGCTGGTGTTTTGAAACAGGCCAAGCAAGTCTCTGTGGAAGAATCGA
AGCCACGGCTGCCCCGACATGTTCGAGATCGTAGTCACTAGATTGGGCGG
TCTGCCTCCATGTTTAGCTTTACTTTTGGCAGGCCATGCTTGACATGGCA
ACGCCACACGTTGCCTAAAACAGCAGGTAGGTTCGCCGAATACCACGATA
CGTGAGGAATCAAACCCCGAAGAATCGCCCGTTCTCGCTTGGTTGGGGGG
TGGCCAAAGACGACTACGTGCCTACCTCCGACGTGATGCTTTGTCGTTGA
GTTTCACCCATCTCTTACTCAAACCTGGCCCCACACCATCCTGGTATTGC
TTCTCTGGCCGTCTTGTTGTTTCTCGCCGGAGTAGATTGTGCTTCAGACG
AAGCGGCTGTTCGTGGGCAGAGTCGTCGCCGCGATCAAGGTGAGGGAGAA
GGAGTTGTCGAGCTGA
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig15175:712..3077-

>mRNA_E-fasciculatus_F_contig15175.3662.1 ID=mRNA_E-fasciculatus_F_contig15175.3662.1|Name=mRNA_E-fasciculatus_F_contig15175.3662.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=1044bp|location=Sequence derived from alignment at E-fasciculatus_F_contig15175:712..3077- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GGGACCAAACAGTCTCTGCTGAACGGCTTCCTCAAGAGACGCAAGCGAGA
CATCGTGCTGGTGGTGCTCACGGTGATTCTCCTGGTCAGCGCGTGGCTGC
TGTGGCCCATCTTGTTCTCACTGAGGGTGGACGGGACGACCTCCATGTCC
TGGGCGGCCGTGTGGACCCCCCTGTGGATTGCCGATGGCATTGGGGACCA
AACAGTCTCTGCTGAACGGCTTCCTCAAGAGACGCAAGCGAGACATCGTG
CTGGTGGTGCTCACGGTGATTCTCCTGGTCAGCGCGTGGCTGCTGTGGCC
CATCTTGTTCTCACTGAGGGTGGACGGGACGACCTCCATGTCCTGGGCGG
CCGTGTGGACCCCCCTGTGGATTGCCGATGGCATTGGGTTGATGTTCTTC
GTATTTCTAGTGAGCTGGGGAGGTATCAAGGCCCCGGCCGGGTGGCAGGG
CGAGTGGAGAGACCCTTACCCGATGTCGATGAGGGTGCTCGCGCTCGTGA
AGTGGGGTTTGTTGGTTGTGTTTCAGGGTTGATGTTCTTCGTATTTCTAG
TGAGCTGGGGAGGTATCAAGGCCCCGGCCGGGTGGCAGGGCGAGTGGAGA
GACCCTTACCCGATGTCGATGAGGGTGCTCGCGCTCGTGAAGTGGGGTTT
GTTGGTTGTGTTTCAGGTGTGCTTAGTTCTCAGGTTGGATCGCCACATCA
GCTGGTCTTGGGAGGAAGTCTTGGCGCCGCTCATGGCGTGGGTCTGGCTT
CGGATTGGTGGAGAGCTGTATAGGGTGTGCTTAGTTCTCAGGTTGGATCG
CCACATCAGCTGGTCTTGGGAGGAAGTCTTGGCGCCGCTCATGGCGTGGG
TCTGGCTTCGGATTGGTGGAGAGCTGTATAGGATTGTGCTTCAGACGAAG
CGGCTGTTCGTGGGCAGAGTCGTCGCCGCGATCAAGGTGAGGGAGAAGGA
GTTGTCGAGCTGAATTGTGCTTCAGACGAAGCGGCTGTTCGTGGGCAGAG
TCGTCGCCGCGATCAAGGTGAGGGAGAAGGAGTTGTCGAGCTGA
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