mRNA_E-fasciculatus_F_contig1458.3299.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig1458.3299.1
Unique NamemRNA_E-fasciculatus_F_contig1458.3299.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig1458.3299.1 vs. uniprot
Match: D7G8R8_ECTSI (CNH domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G8R8_ECTSI)

HSP 1 Score: 437 bits (1124), Expect = 4.700e-141
Identity = 264/303 (87.13%), Postives = 265/303 (87.46%), Query Frame = 1 
Query:    1 GPNTPNKGPTSSAEPPRQLTGVSRSRAASAVMTFLERHRPAIVAATEREDCQKADDAEDADGPAEAAPXXXXXXXXXXXXXADLDGKAGDTLVLLDTMLMSSYVQCSPPRHSALVELLSGPNRCSLEACAPLLAASGPSFIEALLCLYRGRGLHEDALALATEDRQAVIVSWVLLAAVLCVAPSPSSYSXXXXXXXXXXXXXWTAPQFRRWLAAYLRELRLSSEPAHRALVPRSVKPLLEADPALGLSVFLNEHRQAAGLSHHGGLLAQRRVGGSRRAGGASADGRRAEASLAPHDVVSFLKT 909
            GPNTP KGPTSS EPPRQLTGVSRSRAASAVMTFLERHRPAI+AA EREDCQKADDAEDADGPA    XXXXXXXXXXXXX    GKAGDTLVLLDTMLMSSYVQCSPPRHSALVELLSGPNRCSL ACAPLLAASGPSFIEALLCLYRGRGLHEDALALATEDR              CVAPS SS  XXXXXXXXX    WTAPQFRRWLAAYLRELRLSSEPAHRALV RSVKPLLEADPALGLSVFLNEHRQA GLSHHGG LAQRRVG SRRAGGASADGRRAEA LAPHDVVSFLKT
Sbjct:  434 GPNTPKKGPTSSGEPPRQLTGVSRSRAASAVMTFLERHRPAILAAAEREDCQKADDAEDADGPAXXXXXXXXXXXXXXXXXXXXXGKAGDTLVLLDTMLMSSYVQCSPPRHSALVELLSGPNRCSLAACAPLLAASGPSFIEALLCLYRGRGLHEDALALATEDR--------------CVAPSSSSXXXXXXXXXXX----WTAPQFRRWLAAYLRELRLSSEPAHRALVLRSVKPLLEADPALGLSVFLNEHRQATGLSHHGGFLAQRRVGVSRRAGGASADGRRAEAGLAPHDVVSFLKT 718
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BLAST of mRNA_E-fasciculatus_F_contig1458.3299.1 vs. uniprot
Match: A0A6H5L3Y1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L3Y1_9PHAE)

HSP 1 Score: 268 bits (685), Expect = 1.350e-80
Identity = 188/272 (69.12%), Postives = 195/272 (71.69%), Query Frame = 1 
Query:   94 MTFLERHRPAIVAATEREDCQKADDAEDADGPAEAAPXXXXXXXXXXXXXADLDGKAGDTLVLLDTMLMSSYVQCSPPRHSALVELLSGPNRCSLEACAPLLAASGPSFIEALLCLYRGRGLHEDALALATEDRQAVIVSWVLLAAVLCVAPSPSSYSXXXXXXXXXXXXXWTAPQFRRWLAAYLRELRLSSEPAHRALVPRSVKPLLEADPALGLSVFLNEHRQAAGLSHHGGLLAQRRVGGSRRAGGASADGRRAEASLAPHDVVSFLKT 909
            MTFLERHRPAIVAA EREDCQKADD            XXXXXXXXXXXXX     KAGDTLVLLDTMLMSSYVQCSPPRHSALV                      P++   ++   R +    D       D Q  I          CVAPS SS  XXXXXXXXXX   WTAPQFRRWLAAYLRELRLSSEPAHRALVPRSVKPLLEADPALGLSVFL+E+RQAAGLSHHGGLLAQRRVGGSRRAGGASADGRRAEASLAPHDVVSFLKT
Sbjct:    1 MTFLERHRPAIVAAAEREDCQKADDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKAGDTLVLLDTMLMSSYVQCSPPRHSALVR------------------GRAPTYETEIVMKVRLQRAETDG------DEQRSITR--------CVAPSSSSXXXXXXXXXXXX---WTAPQFRRWLAAYLRELRLSSEPAHRALVPRSVKPLLEADPALGLSVFLDENRQAAGLSHHGGLLAQRRVGGSRRAGGASADGRRAEASLAPHDVVSFLKT 237
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BLAST of mRNA_E-fasciculatus_F_contig1458.3299.1 vs. uniprot
Match: A0A835YS55_9STRA (CNH domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YS55_9STRA)

HSP 1 Score: 108 bits (269), Expect = 1.870e-22
Identity = 64/163 (39.26%), Postives = 87/163 (53.37%), Query Frame = 1 
Query:  280 LLDTMLMSSYVQCSPPRHSALVELLSGPNR---CSLEACAPLLAASGPSFIEALLCLYRGRGLHEDALALATEDRQAVIVSWVLLAAVLCVAPSPSSYSXXXXXXXXXXXXXWTAPQFRRWLAAYLRELRLSSEPAHRALVPRSVKPLLEADPALGLSVFLNE 759
            LL+T+L+S+ + C+PPR  A++  LS  +R   C L A AP+LAA+G  F+EALL LYR +GLH +AL L +E+R              CV                     W   Q+RRW+A YL+ L  S +  H  L  R+   LL+ADP LGL+VF  E
Sbjct:  513 LLETVLVSARLHCAPPRREAIIAQLSDASRPCHCDLSATAPVLAAAGAPFVEALLWLYRSKGLHREALGLLSEER--------------CVGAG-----------------GWNKAQYRRWVAQYLQSLWFSDDAEHPPLALRAAPALLQADPRLGLTVFTGE 644
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BLAST of mRNA_E-fasciculatus_F_contig1458.3299.1 vs. uniprot
Match: F0YJL4_AURAN (CNH domain-containing protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YJL4_AURAN)

HSP 1 Score: 99.8 bits (247), Expect = 1.440e-19
Identity = 64/160 (40.00%), Postives = 83/160 (51.88%), Query Frame = 1 
Query:  274 LVLLDTMLMSSYVQCSPPRHSALVELLSGPNR-CSLEACAPLLAASGPSFIEALLCLYRGRGLHEDALALATEDRQAVIVSWVLLAAVLCVAPSPSSYSXXXXXXXXXXXXXWTAPQFRRWLAAYLRELRLSSEPAHRALVPRSVKPLLEADPALGLSVF 750
            LVL+DTML+++++QCSPPR   +V LL  P++ C LE  APLLAA G  + E LL LYR +  H+ ALAL  EDR              C   +  S +             W   +FR W A YLR L  S EP + +L      P+    PALGL+VF
Sbjct:  560 LVLVDTMLIAAWLQCSPPRVEDVVFLLQDPSQQCELEYVAPLLAAGGALYAEPLLWLYRSKQAHQRALALLVEDR-------------CCAENNEGSQA-------------WDRDRFRCWKARYLRWLWFSGEPRNASLAIEHAAPVFSTAPALGLAVF 693
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig1458.3299.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 4
Match NameE-valueIdentityDescription
D7G8R8_ECTSI4.700e-14187.13CNH domain-containing protein n=1 Tax=Ectocarpus s... [more]
A0A6H5L3Y1_9PHAE1.350e-8069.12Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A835YS55_9STRA1.870e-2239.26CNH domain-containing protein n=1 Tax=Tribonema mi... [more]
F0YJL4_AURAN1.440e-1940.00CNH domain-containing protein n=1 Tax=Aureococcus ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig1458contigE-fasciculatus_F_contig1458:535..2857 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score439.1
Seed ortholog evalue1.7e-120
Seed eggNOG ortholog2880.D7G8R8
Preferred nameVPS39
KEGG koko:K17267,ko:K20183
KEGG Pathwayko04138,map04138
GOsGO:0000322,GO:0000323,GO:0000324,GO:0000325,GO:0000329,GO:0003674,GO:0005085,GO:0005088,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005765,GO:0005768,GO:0005770,GO:0005773,GO:0005774,GO:0005794,GO:0005798,GO:0005911,GO:0006810,GO:0006886,GO:0006888,GO:0006890,GO:0006903,GO:0006906,GO:0006914,GO:0006950,GO:0006996,GO:0007033,GO:0007034,GO:0007041,GO:0007154,GO:0008104,GO:0008150,GO:0008152,GO:0008333,GO:0008593,GO:0009056,GO:0009267,GO:0009506,GO:0009605,GO:0009705,GO:0009966,GO:0009967,GO:0009987,GO:0009991,GO:0010008,GO:0010646,GO:0010647,GO:0012505,GO:0012506,GO:0015031,GO:0015833,GO:0016020,GO:0016043,GO:0016050,GO:0016192,GO:0016197,GO:0016236,GO:0016237,GO:0017016,GO:0017112,GO:0017137,GO:0019899,GO:0022406,GO:0022411,GO:0023051,GO:0023056,GO:0030054,GO:0030117,GO:0030120,GO:0030126,GO:0030135,GO:0030137,GO:0030659,GO:0030660,GO:0030662,GO:0030663,GO:0030897,GO:0031090,GO:0031267,GO:0031338,GO:0031410,GO:0031667,GO:0031668,GO:0031669,GO:0031902,GO:0031982,GO:0032006,GO:0032008,GO:0032879,GO:0032889,GO:0032984,GO:0032991,GO:0033036,GO:0033043,GO:0033554,GO:0034058,GO:0034613,GO:0034727,GO:0035542,GO:0042144,GO:0042594,GO:0042886,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043254,GO:0043933,GO:0044087,GO:0044088,GO:0044232,GO:0044237,GO:0044248,GO:0044422,GO:0044424,GO:0044425,GO:0044431,GO:0044433,GO:0044437,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0044804,GO:0045184,GO:0046907,GO:0048193,GO:0048278,GO:0048284,GO:0048475,GO:0048518,GO:0048522,GO:0048583,GO:0048584,GO:0050789,GO:0050794,GO:0050896,GO:0051020,GO:0051049,GO:0051128,GO:0051179,GO:0051234,GO:0051640,GO:0051641,GO:0051648,GO:0051649,GO:0051650,GO:0051656,GO:0051716,GO:0055044,GO:0060627,GO:0061024,GO:0061025,GO:0061919,GO:0065007,GO:0065009,GO:0070727,GO:0071496,GO:0071702,GO:0071705,GO:0071840,GO:0090174,GO:0097352,GO:0097576,GO:0097708,GO:0098588,GO:0098772,GO:0098796,GO:0098805,GO:0098852,GO:0098876,GO:0099022,GO:0099023,GO:0140056,GO:1902500,GO:1902531,GO:1902533,GO:1902774,GO:1903432,GO:1904263,GO:1990126,GO:1990816
EggNOG free text desc.intracellular protein transport
EggNOG OGsCOG5240@1,KOG1078@2759,KOG2063@1,KOG2063@2759
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04131,ko04147
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionCoatomer, gamma subunit, appendage, Ig-like subdomain
Ec32 orthologEc-22_002730.1
Exons5
Model size920
Cds size918
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig1458.3299.1prot_E-fasciculatus_F_contig1458.3299.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig1458 535..2855 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927944.158297-CDS-E-fasciculatus_F_contig1458:534..5971622927944.158297-CDS-E-fasciculatus_F_contig1458:534..597Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1458 535..597 +
1695995760.4696612-CDS-E-fasciculatus_F_contig1458:534..5971695995760.4696612-CDS-E-fasciculatus_F_contig1458:534..597Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1458 535..597 +
1622927944.1691742-CDS-E-fasciculatus_F_contig1458:1062..11941622927944.1691742-CDS-E-fasciculatus_F_contig1458:1062..1194Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1458 1063..1194 +
1695995760.483217-CDS-E-fasciculatus_F_contig1458:1062..11941695995760.483217-CDS-E-fasciculatus_F_contig1458:1062..1194Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1458 1063..1194 +
1622927944.1827366-CDS-E-fasciculatus_F_contig1458:1513..16601622927944.1827366-CDS-E-fasciculatus_F_contig1458:1513..1660Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1458 1514..1660 +
1695995760.4913976-CDS-E-fasciculatus_F_contig1458:1513..16601695995760.4913976-CDS-E-fasciculatus_F_contig1458:1513..1660Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1458 1514..1660 +
1622927944.1966238-CDS-E-fasciculatus_F_contig1458:2026..22201622927944.1966238-CDS-E-fasciculatus_F_contig1458:2026..2220Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1458 2027..2220 +
1695995760.4994779-CDS-E-fasciculatus_F_contig1458:2026..22201695995760.4994779-CDS-E-fasciculatus_F_contig1458:2026..2220Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1458 2027..2220 +
1622927944.2088423-CDS-E-fasciculatus_F_contig1458:2473..28551622927944.2088423-CDS-E-fasciculatus_F_contig1458:2473..2855Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1458 2474..2855 +
1695995760.5073822-CDS-E-fasciculatus_F_contig1458:2473..28551695995760.5073822-CDS-E-fasciculatus_F_contig1458:2473..2855Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1458 2474..2855 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig1458.3299.1

>prot_E-fasciculatus_F_contig1458.3299.1 ID=prot_E-fasciculatus_F_contig1458.3299.1|Name=mRNA_E-fasciculatus_F_contig1458.3299.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=306bp
GPNTPNKGPTSSAEPPRQLTGVSRSRAASAVMTFLERHRPAIVAATERED
CQKADDAEDADGPAEAAPAGGGEGDGGGSGNADLDGKAGDTLVLLDTMLM
SSYVQCSPPRHSALVELLSGPNRCSLEACAPLLAASGPSFIEALLCLYRG
RGLHEDALALATEDRQAVIVSWVLLAAVLCVAPSPSSYSGGSGGGGGGGG
GGWTAPQFRRWLAAYLRELRLSSEPAHRALVPRSVKPLLEADPALGLSVF
LNEHRQAAGLSHHGGLLAQRRVGGSRRAGGASADGRRAEASLAPHDVVSF
LKTXXX
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mRNA from alignment at E-fasciculatus_F_contig1458:535..2857+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig1458.3299.1 ID=mRNA_E-fasciculatus_F_contig1458.3299.1|Name=mRNA_E-fasciculatus_F_contig1458.3299.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=2323bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1458:535..2857+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GGGCCCAATACTCCGAATAAAGGTCCGACATCGTCTGCCGAACCTCCTAG
GCAGCTCACGGGGGTAAGTGGATTACCGCCGGAACAAGGTTGTCTGCATT
TCTCGTAAGCGGCATGCTTTTGTGGGCTTATGTGTGTTTTCTTTATCGTG
TCAGGCGATCGTCTATCTAGTCGAACCTGGTGTGAACCCGTGCCGTCTGC
GATAACGGCAAAATACGCTGCTCGGTACGGTGGGCCGTACGAGAAGGGGT
GCCGTTCAAGTCCTCCCCCGTTGACTTGACGCCTCTCAATAAAGACCTGA
AGGGTCGCCAGGCACTTGATTGTATGCTAATGCTCCTCGAGAACGCATAG
CGGCAGTGAATCAGGTTTAGCGTGCCGCGCGCGGGGCGATCGCAACCAAT
ACAACGGGAGGGAGGCCTCGGAATTTTTTTCTTCCGAGATCGAAAGTATA
GGACATGAGTAGATAGTACGCACGCACTTTTTCCTTGATATTTGGACGTC
TCCACTCGTTGCTGGCGCGTGCCTACAGGTCTCGCGGAGCCGTGCGGCTA
GCGCAGTCATGACGTTTCTCGAGAGACACAGGCCTGCGATAGTGGCCGCG
ACTGAACGGGAGGACTGCCAGAAGGCCGATGATGCCGAAGATGCCGACGG
CCCAGCGGAGGTGTTTTTTTTTTCTCGATGCTACCGCTTTGCCACGGCTA
TCAGTCAGTGAAGGCACACTCCACTTGCACGAACTTCGGACTTACTTACG
CACCGGTGTTCGGGTGTAAGCGTCAGGCGGGCCTTTCCCTCCGGGATGTT
TACTTGGAGGTTTTCTACATATCTTGTACGTCATCTCGAGCGTTTGTTGT
CTTGTTTTCTCTATGACAACACAGTGAAAGACACGCCTCTTGCTTGCTGC
ACCCCCCCCCGTTCTTGGTGCTGCACCCCGATGTTCAATGCCACATTTAT
GTGCTCGGGGGGGGGGTCACTCGAAGCAGGCGGCGCCAGCGGGCGGAGGC
GAAGGCGACGGTGGTGGTTCAGGAAATGCCGACCTCGACGGCAAGGCGGG
AGACACCCTGGTGCTGCTGGACACGATGCTCATGTCGTCTTACGTGCAGT
GCAGCCCGCCGAGACACAGCGCACTGGTACGTACGTAGCAGAGCGCCGAC
CGCGAAACCGAAGTGGGTACAACGAAGGCTTGGCTTTTCAGCGGGCGGAG
AGAGATGACGACGAACAGAAGTGGAGCACAAAACGACACCGAGAACCAAT
GTGAAGCAAGAAGAAGCAAGAAGAACGCAATGTAACCCGTGAGACTTGCA
ATGAGCTCTGGTCCCCGTCGACCTTGGGGAGTATCCTAGCCCACCAGCCT
TGTTCGCACACATCCACAAATCTTCGCTCTCGTTTCTGCAATATGTCCAG
TTGCGACACCATCGAATTTTCCCGTTCAGCACAAACAAACCCACGTATTT
GAAGCCTTTACCGTGCCTTATCGCGAACGCTGCACGAAAAAGGTGGAGCT
TCTGAGCGGCCCCAACCGGTGCAGCCTGGAAGCGTGTGCCCCCCTCTTGG
CCGCCAGCGGTCCGTCTTTCATCGAGGCCCTGCTGTGTCTCTACCGCGGG
AGAGGCCTGCACGAGGACGCGCTGGCGCTTGCCACGGAAGACAGGCAAGC
CGTCATCGTATCCTGGGTCTTGCTTGCTGCTGTGCTGTCGTCATTGTCGT
CTTGCCGTGGCAGGGAGGGAAGTAGAGCGGAGCGTGGCGAGCGAATGTGT
ACGTGTTGTCAAAACGGGGTAGGTGCAGTCCATGGCACTGGCATGTCCAC
CTTCCTCACGAACGGGTGCGCGATCAGCACCGCGCTAGGAATCTCGCGCC
AGGCCTCCCTCCAAGCACCGCGTCTCCCAAACAACCGTCCAGCGTCTTGA
TGACCCATGCCCCGTACCTCTGCAACCGAACAACCTCAGGTGTGTCGCTC
CTTCCCCCTCATCGTACTCCGGGGGCAGCGGCGGCGGCGGCGGCGGCGGC
GGCGGGGGTTGGACGGCGCCGCAGTTCCGCCGGTGGCTCGCCGCCTACCT
CCGAGAGCTCCGGCTCTCCTCGGAACCCGCGCACCGCGCCCTCGTTCCCC
GCTCGGTGAAGCCGCTGCTGGAGGCCGACCCCGCCCTTGGCCTCTCCGTC
TTCTTGAACGAACACCGGCAAGCGGCCGGCCTGAGCCACCATGGCGGTCT
CCTCGCTCAGCGACGGGTAGGAGGTTCCAGGAGGGCCGGGGGGGCTTCGG
CGGACGGGAGACGGGCGGAGGCGAGCCTTGCCCCGCACGACGTGGTGTCG
TTTCTGAAGACCNNNNNNNNNNG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig1458:535..2857+

>mRNA_E-fasciculatus_F_contig1458.3299.1 ID=mRNA_E-fasciculatus_F_contig1458.3299.1|Name=mRNA_E-fasciculatus_F_contig1458.3299.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=1836bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1458:535..2857+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GGGCCCAATACTCCGAATAAAGGTCCGACATCGTCTGCCGAACCTCCTAG
GCAGCTCACGGGGGGGCCCAATACTCCGAATAAAGGTCCGACATCGTCTG
CCGAACCTCCTAGGCAGCTCACGGGGGTCTCGCGGAGCCGTGCGGCTAGC
GCAGTCATGACGTTTCTCGAGAGACACAGGCCTGCGATAGTGGCCGCGAC
TGAACGGGAGGACTGCCAGAAGGCCGATGATGCCGAAGATGCCGACGGCC
CAGCGGAGGTCTCGCGGAGCCGTGCGGCTAGCGCAGTCATGACGTTTCTC
GAGAGACACAGGCCTGCGATAGTGGCCGCGACTGAACGGGAGGACTGCCA
GAAGGCCGATGATGCCGAAGATGCCGACGGCCCAGCGGAGGCGGCGCCAG
CGGGCGGAGGCGAAGGCGACGGTGGTGGTTCAGGAAATGCCGACCTCGAC
GGCAAGGCGGGAGACACCCTGGTGCTGCTGGACACGATGCTCATGTCGTC
TTACGTGCAGTGCAGCCCGCCGAGACACAGCGCACTGGCGGCGCCAGCGG
GCGGAGGCGAAGGCGACGGTGGTGGTTCAGGAAATGCCGACCTCGACGGC
AAGGCGGGAGACACCCTGGTGCTGCTGGACACGATGCTCATGTCGTCTTA
CGTGCAGTGCAGCCCGCCGAGACACAGCGCACTGGTGGAGCTTCTGAGCG
GCCCCAACCGGTGCAGCCTGGAAGCGTGTGCCCCCCTCTTGGCCGCCAGC
GGTCCGTCTTTCATCGAGGCCCTGCTGTGTCTCTACCGCGGGAGAGGCCT
GCACGAGGACGCGCTGGCGCTTGCCACGGAAGACAGGCAAGCCGTCATCG
TATCCTGGGTCTTGCTTGCTGCTGTGCTGTGGAGCTTCTGAGCGGCCCCA
ACCGGTGCAGCCTGGAAGCGTGTGCCCCCCTCTTGGCCGCCAGCGGTCCG
TCTTTCATCGAGGCCCTGCTGTGTCTCTACCGCGGGAGAGGCCTGCACGA
GGACGCGCTGGCGCTTGCCACGGAAGACAGGCAAGCCGTCATCGTATCCT
GGGTCTTGCTTGCTGCTGTGCTGTGTGTCGCTCCTTCCCCCTCATCGTAC
TCCGGGGGCAGCGGCGGCGGCGGCGGCGGCGGCGGCGGGGGTTGGACGGC
GCCGCAGTTCCGCCGGTGGCTCGCCGCCTACCTCCGAGAGCTCCGGCTCT
CCTCGGAACCCGCGCACCGCGCCCTCGTTCCCCGCTCGGTGAAGCCGCTG
CTGGAGGCCGACCCCGCCCTTGGCCTCTCCGTCTTCTTGAACGAACACCG
GCAAGCGGCCGGCCTGAGCCACCATGGCGGTCTCCTCGCTCAGCGACGGG
TAGGAGGTTCCAGGAGGGCCGGGGGGGCTTCGGCGGACGGGAGACGGGCG
GAGGCGAGCCTTGCCCCGCACGACGTGGTGTCGTTTCTGAAGACCNNNNN
NNNNGTGTGTCGCTCCTTCCCCCTCATCGTACTCCGGGGGCAGCGGCGGC
GGCGGCGGCGGCGGCGGCGGGGGTTGGACGGCGCCGCAGTTCCGCCGGTG
GCTCGCCGCCTACCTCCGAGAGCTCCGGCTCTCCTCGGAACCCGCGCACC
GCGCCCTCGTTCCCCGCTCGGTGAAGCCGCTGCTGGAGGCCGACCCCGCC
CTTGGCCTCTCCGTCTTCTTGAACGAACACCGGCAAGCGGCCGGCCTGAG
CCACCATGGCGGTCTCCTCGCTCAGCGACGGGTAGGAGGTTCCAGGAGGG
CCGGGGGGGCTTCGGCGGACGGGAGACGGGCGGAGGCGAGCCTTGCCCCG
CACGACGTGGTGTCGTTTCTGAAGACCNNNNNNNNN
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