mRNA_E-fasciculatus_F_contig1434.3161.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_E-fasciculatus_F_contig1434.3161.1
Unique Name	mRNA_E-fasciculatus_F_contig1434.3161.1
Type	mRNA
Organism	Ectocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)

Homology

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BLAST of mRNA_E-fasciculatus_F_contig1434.3161.1 vs. uniprot
Match: A0A6H5KSL4_9PHAE (PNPLA domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KSL4_9PHAE)

HSP 1 Score: 675 bits (1742), Expect = 6.210e-219
Identity = 465/629 (73.93%), Postives = 490/629 (77.90%), Query Frame = 1

Query:  871 MVAAPLGGERSTGGKHQRNPGSWDPSTSSLPMLPPAREPSDASASQPPSSPLESLPATSTTKESQLQXXXXXAEEGNQVVDAVLYAIKLGWPWSQVAALGEKVVLSPQLQQQXXXXXXXXSRIVXXXXXXXXLGVKLGWPWTPESTSPAPAAPVGKELTMREAPPLGGERKELPSSLPMVPPGPPREEEEGVGGSGPPARGGRRFSRAKEAI-GTVLGSRLLRKTTS----AGAPGATTKRSSIADDA-PLPRVPGAPAAPPRSAAPTLSPPPAAAALRGNDGSSAGPAAPAGRVPWRPPGYRWGKSLISNMSGLPDADGERLTATTDXXXXXXXGRQVAGVGAASAGTGWSXXXXXXXXXXEKGREAEATGGVQGQQEEALQQQLSSRRMRVFRALSTTAGGVASRLPLMKKERNENAREEDVSDQAYNRNTVGEWASVEVSPLSSTKADAAEGGANAARGGGGVPWWQAGAAVLVGVGAALYRPVNRLPGLLLKDEXXXXXXXXXXXXGRQRGTLSSVDATASPATFPLVRPAEEVASVPPEPRXXXXXXXXXXXXXXXXSSRSRARQGVAGLVDRAATMSRRARLWTLLKRRSGARAEPLLASVDTSIMSLTPSELLSPPAEAEET 2739
            MVAAPLG ERSTGGK QRNPGSW  STSSLPMLPPAR+PSD SA +PP                   XXXXXAEEGNQVVDAVLYAI LGW WSQVAALGEKVV SPQL   XXXXXXXX    XXXXXXXXLGVKLGWPWTPESTSPA AAP  + + M       G  KELP SLP VPP  PREE  GV  SGPP RGGR FSR KEAI GTVLGSRLLR TT+    AGAPGATTKRS  A +A PL  V GAPAAPPRSAAPTLS PPAAAALR  DGSS GPA PAGR PWRPPGYRWGKSL+SNMSGLPD + ER+TA TDXXXXXXXGRQVAGVGAASAGTGWS   XXXX   E+G + EAT GVQGQQEEA QQQLS+RRM VFRAL+TTAGGVA+RLPLMKK R+E+A+E+D  DQAY RN+VGEWASVEVSP +S  A+ A+G          VPWWQAGAAVLVGVGAALYRPVNRLPGLLLKDE  XXXXXX    GRQ+GTLS VDAT SPA+F L RPAEE A VPPEPR   XXXX         SS SRARQGVAGLVDRAATMSRRARLWTLLKRRS ARAEPLLASVDTSIMSLTPSELLSPP E +ET
Sbjct:    1 MVAAPLGSERSTGGKQQRNPGSWGSSTSSLPMLPPARQPSDVSAPRPPXXXXXXXXXXXXXXXXXXXXXXXXAEEGNQVVDAVLYAINLGWLWSQVAALGEKVV-SPQLXXXXXXXXXXXXXXXXXXXXXXXLGVKLGWPWTPESTSPAAAAPDQEGVMM-------GASKELPLSLPTVPPDLPREE--GVSDSGPPTRGGRSFSRVKEAIVGTVLGSRLLRTTTTMTTTAGAPGATTKRSCTAHEAAPLTSVAGAPAAPPRSAAPTLSLPPAAAALRRTDGSSPGPA-PAGRAPWRPPGYRWGKSLVSNMSGLPDGESERVTAATDXXXXXXXGRQVAGVGAASAGTGWSPSAXXXXD--EQGGKTEATRGVQGQQEEAQQQQLSARRMSVFRALATTAGGVATRLPLMKKSRSEDAQEQDSPDQAYKRNSVGEWASVEVSPPASKGANGAQGXXXXXXXXX-VPWWQAGAAVLVGVGAALYRPVNRLPGLLLKDEKEXXXXXXQGEEGRQQGTLSLVDATKSPASFQLARPAEEGALVPPEPRPNPXXXXPPAAGTT--SSGSRARQGVAGLVDRAATMSRRARLWTLLKRRSRARAEPLLASVDTSIMSLTPSELLSPPVEEQET 613

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BLAST of mRNA_E-fasciculatus_F_contig1434.3161.1 vs. uniprot
Match: D7FZF1_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FZF1_ECTSI)

HSP 1 Score: 634 bits (1635), Expect = 1.300e-211
Identity = 443/603 (73.47%), Postives = 465/603 (77.11%), Query Frame = 1

Query:  964 MLPPAREPSDASASQPPSSPLESLPATSTTKESQLQXXXXXAEEGNQVVDAVLYAIKLGWPWSQVAALGEKVVLSPQLQQQXXXXXXXXSRIVXXXXXXXXLGVKLGWPWTPESTSPAPAAPVGKELTMREAPPLGGERKELPSSLPMVPPGPPREEEEGVGGSGPPARGGRRFSRAKEAI-GTVLGSRLLRKTTSAG-APGATTKRSSIADDA-PLPRVPGAPAAPPRSAAPTLSPPPAAAALRGNDGSSAGPAAPAGRVPWRPPGYRWGKSLISNMSGLPDADGERLTATTDXXXXXXXGRQVAGVGAASAGTGWSXXXXXXXXXXEKGREAEATGGVQGQQEEALQQQLSSRRMRVFRALSTTAGGVASRLPLMKKERNENAREEDVSDQAYNRNTVGEWASVEVSPLSSTKADAAEGGANAARGGGG--------VPWWQAGAAVLVGVGAALYRPVNRLPGLLLKDEXXXXXXXXXXXXGRQRGTLSSVDATASPATFPLVRPAEEVASVPPEPRXXXXXXXXXXXXXXXXSSRSRARQGVAGLVDRAATMSRRARLWTLLKRRSGARAEPLLASVDTSIMSLTPSELLSPPAEAEET 2739
            MLPPAREPSD SAS+ P SPLES P           XXXXXA EGNQVVDAVLYAI LGWPWSQVAALGEKVV SPQL   XXXXXXXX    XXXX    LGVKLGWPWTPESTSPA AAP  + +     PPL GE KELP S P VPP PP+EEE GVG SGPP RGGR FSR KEAI GTVLGSRLLR TT+ G APGA TKRSS ADDA PLP V GAPAAPPRSAAPTLS PPAAAALR  DGSSAGPA PAGR PWRPPGYRWGKSL+SNMSGLP  + ER+TATT XXXXXXX                 XXXXXXXXXX     A  TGGVQGQQEEA Q+QLSSRRM VFRALSTTAGGVA+RLPLMKK R+++ARE+D+ DQAYNRN+VGEWASVEVSP +STKADAAEGGAN A+            VPWWQAGAAVLVGVGAALYRPVNRLPGLL KDE            GRQ+GTLS VDAT SPA+F L RPAEEVASVPPEPR   XXXXXXXXX    +S SRARQGVAGLVDRAATMSRRARLWTLLKRRS ARAEPLL SVDTSIMSLTPSELLSPP E +ET
Sbjct:    1 MLPPAREPSDVSASRQPPSPLES-PXXXXXXXXXXXXXXXXAGEGNQVVDAVLYAINLGWPWSQVAALGEKVV-SPQLXXXXXXXXXXXXXXXXXXXGMIKLGVKLGWPWTPESTSPAAAAPNQEGVMTGATPPLDGESKELPLSFPQVPPDPPQEEE-GVGDSGPPTRGGRSFSRVKEAIVGTVLGSRLLRTTTTTGGAPGAITKRSSTADDAAPLPSVAGAPAAPPRSAAPTLSLPPAAAALRRIDGSSAGPA-PAGRAPWRPPGYRWGKSLVSNMSGLPGVESERVTATTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXA--TGGVQGQQEEARQRQLSSRRMSVFRALSTTAGGVATRLPLMKKSRSDDAREQDLPDQAYNRNSVGEWASVEVSPPASTKADAAEGGANGAQXXXXXXXXXXXXVPWWQAGAAVLVGVGAALYRPVNRLPGLLFKDEKEEEQRRRQGEEGRQQGTLSQVDATTSPASFQLARPAEEVASVPPEPRPNPXXXXXXXXXTT--TSGSRARQGVAGLVDRAATMSRRARLWTLLKRRSRARAEPLLTSVDTSIMSLTPSELLSPPVEEQET 595

The following BLAST results are available for this feature:

BLAST of mRNA_E-fasciculatus_F_contig1434.3161.1 vs. uniprot

BLAST of mRNA_E-fasciculatus_F_contig1434.3161.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 2


Match Name	E-value	Identity	Description
A0A6H5KSL4_9PHAE	6.210e-219	73.93	PNPLA domain-containing protein n=1 Tax=Ectocarpus...	[more]
D7FZF1_ECTSI	1.300e-211	73.47	Uncharacterized protein n=1 Tax=Ectocarpus silicul...	[more]

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
E-fasciculatus_F_contig1434	contig	E-fasciculatus_F_contig1434:15272..18082 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90	2022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female	2021-02-24

Properties


Property Name	Value
Hectar predicted targeting category	chloroplast
Ec32 ortholog description	expressed unknown protein
Ec32 ortholog	Ec-27_001580.1
Exons	1
Model size	2813
Cds size	2811
Stop	0
Start	0

Relationships

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1622927924.2235193-CDS-E-fasciculatus_F_contig1434:15271..18082	1622927924.2235193-CDS-E-fasciculatus_F_contig1434:15271..18082	Ectocarpus fasciculatus Ec846f_Ec191_B4_f female	CDS	E-fasciculatus_F_contig1434 15272..18082 +
1695995752.9125242-CDS-E-fasciculatus_F_contig1434:15271..18082	1695995752.9125242-CDS-E-fasciculatus_F_contig1434:15271..18082	Ectocarpus fasciculatus Ec846f_Ec191_B4_f female	CDS	E-fasciculatus_F_contig1434 15272..18082 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_E-fasciculatus_F_contig1434.3161.1	prot_E-fasciculatus_F_contig1434.3161.1	Ectocarpus fasciculatus Ec846f_Ec191_B4_f female	polypeptide	E-fasciculatus_F_contig1434 15272..18082 +

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at E-fasciculatus_F_contig1434:15272..18082+
coding sequence from alignment at E-fasciculatus_F_contig1434:15272..18082+

protein sequence of mRNA_E-fasciculatus_F_contig1434.3161.1

>prot_E-fasciculatus_F_contig1434.3161.1 ID=prot_E-fasciculatus_F_contig1434.3161.1|Name=mRNA_E-fasciculatus_F_contig1434.3161.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=937bp
ASADKEWYVSRRTMWAAWELLLLCLVGGAGATLLGPISAFVCTPRLPRSP
DVVRITQEQQHACQSIARKSVVSSPLSVRPTTRASPTVSHSLRMGGGNWG
RRPSKTEGRMGMNAVSNSNSNSNSNNSNDSNGSTSTSTTTTANDNSSTGD
EIGRNTVKTPPDVQAGGADKSGGLGVAFVGLAKVSKSIKPPTRVEGLQQQ
GQYKKRKKKPSRPLSSGSPLLSKAAIPERIPLDDDVEAEPPPPPPPPVKV
SPGADRRGGWKWIIPPWHNRKQTVPDTCEPAIAPDAASATMVAAPLGGER
STGGKHQRNPGSWDPSTSSLPMLPPAREPSDASASQPPSSPLESLPATST
TKESQLQQQQEEAEEGNQVVDAVLYAIKLGWPWSQVAALGEKVVLSPQLQ
QQQAEMSEEVSRIVESMLGMIKLGVKLGWPWTPESTSPAPAAPVGKELTM
REAPPLGGERKELPSSLPMVPPGPPREEEEGVGGSGPPARGGRRFSRAKE
AIGTVLGSRLLRKTTSAGAPGATTKRSSIADDAPLPRVPGAPAAPPRSAA
PTLSPPPAAAALRGNDGSSAGPAAPAGRVPWRPPGYRWGKSLISNMSGLP
DADGERLTATTDDGGGSSSGRQVAGVGAASAGTGWSPSAAAAAAADEKGR
EAEATGGVQGQQEEALQQQLSSRRMRVFRALSTTAGGVASRLPLMKKERN
ENAREEDVSDQAYNRNTVGEWASVEVSPLSSTKADAAEGGANAARGGGGV
PWWQAGAAVLVGVGAALYRPVNRLPGLLLKDEKEEERRRQQEEEGRQRGT
LSSVDATASPATFPLVRPAEEVASVPPEPRPNPPPQEQPPPAEVTTSSRS
RARQGVAGLVDRAATMSRRARLWTLLKRRSGARAEPLLASVDTSIMSLTP
SELLSPPAEAEETGGSLLSSSSGGGAEESVGGGGGGG

mRNA from alignment at E-fasciculatus_F_contig1434:15272..18082+

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_E-fasciculatus_F_contig1434.3161.1 ID=mRNA_E-fasciculatus_F_contig1434.3161.1|Name=mRNA_E-fasciculatus_F_contig1434.3161.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=2811bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1434:15272..18082+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GCATCGGCGGACAAGGAGTGGTATGTATCAAGGAGGACCATGTGGGCGGC
ATGGGAACTACTGCTCCTGTGCCTTGTTGGAGGTGCAGGAGCGACATTGC
TCGGGCCAATCTCTGCCTTTGTTTGCACCCCAAGGCTGCCGAGATCACCT
GACGTGGTTCGGATCACCCAGGAGCAGCAGCACGCGTGTCAATCCATCGC
TCGGAAGAGCGTTGTCAGCTCACCCTTGTCAGTGAGACCCACGACCAGAG
CCTCTCCCACCGTGTCGCATTCGTTGCGCATGGGCGGTGGCAATTGGGGT
CGAAGGCCGTCCAAAACGGAAGGCAGGATGGGCATGAATGCTGTGAGCAA
CAGCAACAGCAACAGCAACAGCAACAACAGCAACGACAGCAACGGCAGCA
CCAGCACCAGCACCACTACCACCGCGAACGACAACAGCAGTACAGGGGAC
GAAATTGGACGAAACACAGTAAAAACACCTCCAGACGTACAAGCGGGAGG
TGCGGACAAAAGCGGTGGCCTCGGTGTTGCTTTTGTTGGCCTGGCTAAGG
TGTCAAAGTCGATCAAGCCGCCAACACGTGTTGAAGGCCTGCAGCAGCAA
GGCCAGTACAAGAAGAGGAAGAAGAAGCCGTCTCGCCCTCTGAGTAGTGG
CAGCCCATTGCTTTCGAAGGCAGCGATTCCCGAACGTATCCCCCTCGACG
ACGATGTGGAGGCAGAACCACCACCACCACCACCACCACCAGTAAAAGTA
TCACCAGGGGCAGATAGACGTGGGGGCTGGAAATGGATAATACCTCCATG
GCACAACCGAAAGCAGACCGTTCCAGACACGTGCGAGCCTGCCATAGCGC
CGGACGCAGCGAGCGCGACGATGGTAGCGGCTCCCCTGGGGGGTGAAAGG
AGCACCGGCGGCAAGCACCAACGCAATCCTGGATCGTGGGACCCCTCGAC
CTCTTCGCTTCCCATGCTCCCGCCGGCACGGGAGCCGAGCGATGCCTCGG
CTTCGCAACCGCCTTCTTCTCCGCTGGAATCACTCCCGGCCACCTCCACG
ACCAAGGAGAGCCAGCTGCAGCAGCAGCAGGAGGAGGCCGAAGAAGGGAA
CCAGGTTGTCGATGCGGTGTTGTACGCGATCAAACTCGGGTGGCCGTGGT
CTCAAGTCGCCGCCCTCGGCGAGAAGGTCGTGTTATCGCCGCAGCTGCAG
CAGCAGCAGGCGGAGATGTCGGAGGAGGTGAGTCGAATTGTCGAATCGAT
GCTGGGCATGATAAAGCTCGGCGTCAAGCTTGGCTGGCCGTGGACTCCGG
AGAGCACAAGTCCGGCGCCCGCCGCTCCGGTCGGAAAAGAGTTGACGATG
AGGGAAGCGCCGCCGCTGGGCGGTGAAAGGAAGGAGCTGCCCTCGAGTTT
GCCCATGGTTCCTCCCGGCCCGCCGCGAGAAGAAGAAGAGGGGGTGGGCG
GTAGCGGGCCTCCTGCGCGCGGTGGCCGACGCTTTTCCCGCGCGAAAGAA
GCCATCGGGACGGTGCTCGGCTCTCGGCTCCTCCGCAAGACGACGTCGGC
CGGTGCCCCAGGGGCAACAACGAAGCGGAGTTCCATCGCGGATGATGCTC
CTCTCCCGCGCGTGCCCGGCGCACCCGCTGCGCCGCCGCGTTCTGCTGCC
CCGACGCTTTCGCCCCCACCGGCCGCTGCAGCGCTGCGGGGGAATGACGG
TAGCAGTGCCGGCCCAGCAGCCCCCGCCGGCCGCGTGCCGTGGCGACCGC
CGGGCTACCGCTGGGGCAAATCCTTAATCTCCAACATGAGCGGCTTACCG
GATGCAGATGGCGAGCGGTTGACGGCCACGACCGACGACGGCGGCGGCAG
TAGTAGCGGGAGGCAGGTCGCGGGGGTGGGCGCAGCCTCTGCTGGTACGG
GCTGGTCGCCGTCGGCTGCTGCGGCGGCGGCGGCGGACGAAAAAGGGAGG
GAAGCCGAGGCGACAGGGGGTGTCCAAGGGCAGCAAGAAGAGGCGCTGCA
GCAGCAGCTGTCATCGAGGAGAATGCGCGTTTTTCGTGCCCTCTCTACGA
CGGCCGGCGGGGTCGCAAGTCGACTCCCTTTGATGAAAAAAGAGCGCAAC
GAAAACGCGCGGGAGGAGGACGTGTCCGATCAGGCGTACAACAGGAACAC
TGTTGGCGAGTGGGCGTCGGTTGAGGTTTCGCCGCTTTCCTCCACCAAAG
CAGACGCGGCGGAAGGAGGCGCCAACGCGGCTCGGGGCGGCGGCGGCGTG
CCTTGGTGGCAGGCAGGGGCCGCGGTGCTCGTTGGGGTCGGGGCCGCGCT
TTACAGACCCGTCAACAGACTGCCGGGATTGTTGCTCAAGGATGAGAAGG
AGGAAGAACGGCGACGACAACAAGAGGAGGAGGGGCGGCAACGGGGAACG
CTTTCGAGCGTGGACGCTACTGCGAGTCCAGCGACGTTCCCGTTGGTTCG
TCCGGCCGAAGAGGTAGCGTCGGTGCCACCGGAACCGCGGCCGAACCCGC
CACCGCAGGAGCAACCGCCGCCGGCGGAGGTGACGACCAGCTCCCGTTCG
CGCGCTCGGCAGGGGGTAGCAGGCTTGGTGGACAGGGCGGCGACAATGTC
GAGGAGGGCGAGGCTGTGGACGCTCTTGAAGCGCAGGTCGGGTGCTCGCG
CGGAGCCCCTTCTGGCGAGCGTGGACACGAGCATCATGTCGCTGACGCCG
TCCGAGCTTCTGTCGCCACCGGCAGAAGCAGAAGAGACCGGCGGTTCCTT
ACTGTCATCTTCGAGCGGCGGGGGGGCCGAGGAGTCGGTAGGTGGGGGGG
GGGGGGGGGGC

Coding sequence (CDS) from alignment at E-fasciculatus_F_contig1434:15272..18082+

>mRNA_E-fasciculatus_F_contig1434.3161.1 ID=mRNA_E-fasciculatus_F_contig1434.3161.1|Name=mRNA_E-fasciculatus_F_contig1434.3161.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=5622bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1434:15272..18082+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GCATCGGCGGACAAGGAGTGGTATGTATCAAGGAGGACCATGTGGGCGGC
ATGGGAACTACTGCTCCTGTGCCTTGTTGGAGGTGCAGGAGCGACATTGC
TCGGGCCAATCTCTGCCTTTGTTTGCACCCCAAGGCTGCCGAGATCACCT
GACGTGGTTCGGATCACCCAGGAGCAGCAGCACGCGTGTCAATCCATCGC
TCGGAAGAGCGTTGTCAGCTCACCCTTGTCAGTGAGACCCACGACCAGAG
CCTCTCCCACCGTGTCGCATTCGTTGCGCATGGGCGGTGGCAATTGGGGT
CGAAGGCCGTCCAAAACGGAAGGCAGGATGGGCATGAATGCTGTGAGCAA
CAGCAACAGCAACAGCAACAGCAACAACAGCAACGACAGCAACGGCAGCA
CCAGCACCAGCACCACTACCACCGCGAACGACAACAGCAGTACAGGGGAC
GAAATTGGACGAAACACAGTAAAAACACCTCCAGACGTACAAGCGGGAGG
TGCGGACAAAAGCGGTGGCCTCGGTGTTGCTTTTGTTGGCCTGGCTAAGG
TGTCAAAGTCGATCAAGCCGCCAACACGTGTTGAAGGCCTGCAGCAGCAA
GGCCAGTACAAGAAGAGGAAGAAGAAGCCGTCTCGCCCTCTGAGTAGTGG
CAGCCCATTGCTTTCGAAGGCAGCGATTCCCGAACGTATCCCCCTCGACG
ACGATGTGGAGGCAGAACCACCACCACCACCACCACCACCAGTAAAAGTA
TCACCAGGGGCAGATAGACGTGGGGGCTGGAAATGGATAATACCTCCATG
GCACAACCGAAAGCAGACCGTTCCAGACACGTGCGAGCCTGCCATAGCGC
CGGACGCAGCGAGCGCGACGATGGTAGCGGCTCCCCTGGGGGGTGAAAGG
AGCACCGGCGGCAAGCACCAACGCAATCCTGGATCGTGGGACCCCTCGAC
CTCTTCGCTTCCCATGCTCCCGCCGGCACGGGAGCCGAGCGATGCCTCGG
CTTCGCAACCGCCTTCTTCTCCGCTGGAATCACTCCCGGCCACCTCCACG
ACCAAGGAGAGCCAGCTGCAGCAGCAGCAGGAGGAGGCCGAAGAAGGGAA
CCAGGTTGTCGATGCGGTGTTGTACGCGATCAAACTCGGGTGGCCGTGGT
CTCAAGTCGCCGCCCTCGGCGAGAAGGTCGTGTTATCGCCGCAGCTGCAG
CAGCAGCAGGCGGAGATGTCGGAGGAGGTGAGTCGAATTGTCGAATCGAT
GCTGGGCATGATAAAGCTCGGCGTCAAGCTTGGCTGGCCGTGGACTCCGG
AGAGCACAAGTCCGGCGCCCGCCGCTCCGGTCGGAAAAGAGTTGACGATG
AGGGAAGCGCCGCCGCTGGGCGGTGAAAGGAAGGAGCTGCCCTCGAGTTT
GCCCATGGTTCCTCCCGGCCCGCCGCGAGAAGAAGAAGAGGGGGTGGGCG
GTAGCGGGCCTCCTGCGCGCGGTGGCCGACGCTTTTCCCGCGCGAAAGAA
GCCATCGGGACGGTGCTCGGCTCTCGGCTCCTCCGCAAGACGACGTCGGC
CGGTGCCCCAGGGGCAACAACGAAGCGGAGTTCCATCGCGGATGATGCTC
CTCTCCCGCGCGTGCCCGGCGCACCCGCTGCGCCGCCGCGTTCTGCTGCC
CCGACGCTTTCGCCCCCACCGGCCGCTGCAGCGCTGCGGGGGAATGACGG
TAGCAGTGCCGGCCCAGCAGCCCCCGCCGGCCGCGTGCCGTGGCGACCGC
CGGGCTACCGCTGGGGCAAATCCTTAATCTCCAACATGAGCGGCTTACCG
GATGCAGATGGCGAGCGGTTGACGGCCACGACCGACGACGGCGGCGGCAG
TAGTAGCGGGAGGCAGGTCGCGGGGGTGGGCGCAGCCTCTGCTGGTACGG
GCTGGTCGCCGTCGGCTGCTGCGGCGGCGGCGGCGGACGAAAAAGGGAGG
GAAGCCGAGGCGACAGGGGGTGTCCAAGGGCAGCAAGAAGAGGCGCTGCA
GCAGCAGCTGTCATCGAGGAGAATGCGCGTTTTTCGTGCCCTCTCTACGA
CGGCCGGCGGGGTCGCAAGTCGACTCCCTTTGATGAAAAAAGAGCGCAAC
GAAAACGCGCGGGAGGAGGACGTGTCCGATCAGGCGTACAACAGGAACAC
TGTTGGCGAGTGGGCGTCGGTTGAGGTTTCGCCGCTTTCCTCCACCAAAG
CAGACGCGGCGGAAGGAGGCGCCAACGCGGCTCGGGGCGGCGGCGGCGTG
CCTTGGTGGCAGGCAGGGGCCGCGGTGCTCGTTGGGGTCGGGGCCGCGCT
TTACAGACCCGTCAACAGACTGCCGGGATTGTTGCTCAAGGATGAGAAGG
AGGAAGAACGGCGACGACAACAAGAGGAGGAGGGGCGGCAACGGGGAACG
CTTTCGAGCGTGGACGCTACTGCGAGTCCAGCGACGTTCCCGTTGGTTCG
TCCGGCCGAAGAGGTAGCGTCGGTGCCACCGGAACCGCGGCCGAACCCGC
CACCGCAGGAGCAACCGCCGCCGGCGGAGGTGACGACCAGCTCCCGTTCG
CGCGCTCGGCAGGGGGTAGCAGGCTTGGTGGACAGGGCGGCGACAATGTC
GAGGAGGGCGAGGCTGTGGACGCTCTTGAAGCGCAGGTCGGGTGCTCGCG
CGGAGCCCCTTCTGGCGAGCGTGGACACGAGCATCATGTCGCTGACGCCG
TCCGAGCTTCTGTCGCCACCGGCAGAAGCAGAAGAGACCGGCGGTTCCTT
ACTGTCATCTTCGAGCGGCGGGGGGGCCGAGGAGTCGGTAGGTGGGGGGG
GGGGGGGGGGCGCATCGGCGGACAAGGAGTGGTATGTATCAAGGAGGACC
ATGTGGGCGGCATGGGAACTACTGCTCCTGTGCCTTGTTGGAGGTGCAGG
AGCGACATTGCTCGGGCCAATCTCTGCCTTTGTTTGCACCCCAAGGCTGC
CGAGATCACCTGACGTGGTTCGGATCACCCAGGAGCAGCAGCACGCGTGT
CAATCCATCGCTCGGAAGAGCGTTGTCAGCTCACCCTTGTCAGTGAGACC
CACGACCAGAGCCTCTCCCACCGTGTCGCATTCGTTGCGCATGGGCGGTG
GCAATTGGGGTCGAAGGCCGTCCAAAACGGAAGGCAGGATGGGCATGAAT
GCTGTGAGCAACAGCAACAGCAACAGCAACAGCAACAACAGCAACGACAG
CAACGGCAGCACCAGCACCAGCACCACTACCACCGCGAACGACAACAGCA
GTACAGGGGACGAAATTGGACGAAACACAGTAAAAACACCTCCAGACGTA
CAAGCGGGAGGTGCGGACAAAAGCGGTGGCCTCGGTGTTGCTTTTGTTGG
CCTGGCTAAGGTGTCAAAGTCGATCAAGCCGCCAACACGTGTTGAAGGCC
TGCAGCAGCAAGGCCAGTACAAGAAGAGGAAGAAGAAGCCGTCTCGCCCT
CTGAGTAGTGGCAGCCCATTGCTTTCGAAGGCAGCGATTCCCGAACGTAT
CCCCCTCGACGACGATGTGGAGGCAGAACCACCACCACCACCACCACCAC
CAGTAAAAGTATCACCAGGGGCAGATAGACGTGGGGGCTGGAAATGGATA
ATACCTCCATGGCACAACCGAAAGCAGACCGTTCCAGACACGTGCGAGCC
TGCCATAGCGCCGGACGCAGCGAGCGCGACGATGGTAGCGGCTCCCCTGG
GGGGTGAAAGGAGCACCGGCGGCAAGCACCAACGCAATCCTGGATCGTGG
GACCCCTCGACCTCTTCGCTTCCCATGCTCCCGCCGGCACGGGAGCCGAG
CGATGCCTCGGCTTCGCAACCGCCTTCTTCTCCGCTGGAATCACTCCCGG
CCACCTCCACGACCAAGGAGAGCCAGCTGCAGCAGCAGCAGGAGGAGGCC
GAAGAAGGGAACCAGGTTGTCGATGCGGTGTTGTACGCGATCAAACTCGG
GTGGCCGTGGTCTCAAGTCGCCGCCCTCGGCGAGAAGGTCGTGTTATCGC
CGCAGCTGCAGCAGCAGCAGGCGGAGATGTCGGAGGAGGTGAGTCGAATT
GTCGAATCGATGCTGGGCATGATAAAGCTCGGCGTCAAGCTTGGCTGGCC
GTGGACTCCGGAGAGCACAAGTCCGGCGCCCGCCGCTCCGGTCGGAAAAG
AGTTGACGATGAGGGAAGCGCCGCCGCTGGGCGGTGAAAGGAAGGAGCTG
CCCTCGAGTTTGCCCATGGTTCCTCCCGGCCCGCCGCGAGAAGAAGAAGA
GGGGGTGGGCGGTAGCGGGCCTCCTGCGCGCGGTGGCCGACGCTTTTCCC
GCGCGAAAGAAGCCATCGGGACGGTGCTCGGCTCTCGGCTCCTCCGCAAG
ACGACGTCGGCCGGTGCCCCAGGGGCAACAACGAAGCGGAGTTCCATCGC
GGATGATGCTCCTCTCCCGCGCGTGCCCGGCGCACCCGCTGCGCCGCCGC
GTTCTGCTGCCCCGACGCTTTCGCCCCCACCGGCCGCTGCAGCGCTGCGG
GGGAATGACGGTAGCAGTGCCGGCCCAGCAGCCCCCGCCGGCCGCGTGCC
GTGGCGACCGCCGGGCTACCGCTGGGGCAAATCCTTAATCTCCAACATGA
GCGGCTTACCGGATGCAGATGGCGAGCGGTTGACGGCCACGACCGACGAC
GGCGGCGGCAGTAGTAGCGGGAGGCAGGTCGCGGGGGTGGGCGCAGCCTC
TGCTGGTACGGGCTGGTCGCCGTCGGCTGCTGCGGCGGCGGCGGCGGACG
AAAAAGGGAGGGAAGCCGAGGCGACAGGGGGTGTCCAAGGGCAGCAAGAA
GAGGCGCTGCAGCAGCAGCTGTCATCGAGGAGAATGCGCGTTTTTCGTGC
CCTCTCTACGACGGCCGGCGGGGTCGCAAGTCGACTCCCTTTGATGAAAA
AAGAGCGCAACGAAAACGCGCGGGAGGAGGACGTGTCCGATCAGGCGTAC
AACAGGAACACTGTTGGCGAGTGGGCGTCGGTTGAGGTTTCGCCGCTTTC
CTCCACCAAAGCAGACGCGGCGGAAGGAGGCGCCAACGCGGCTCGGGGCG
GCGGCGGCGTGCCTTGGTGGCAGGCAGGGGCCGCGGTGCTCGTTGGGGTC
GGGGCCGCGCTTTACAGACCCGTCAACAGACTGCCGGGATTGTTGCTCAA
GGATGAGAAGGAGGAAGAACGGCGACGACAACAAGAGGAGGAGGGGCGGC
AACGGGGAACGCTTTCGAGCGTGGACGCTACTGCGAGTCCAGCGACGTTC
CCGTTGGTTCGTCCGGCCGAAGAGGTAGCGTCGGTGCCACCGGAACCGCG
GCCGAACCCGCCACCGCAGGAGCAACCGCCGCCGGCGGAGGTGACGACCA
GCTCCCGTTCGCGCGCTCGGCAGGGGGTAGCAGGCTTGGTGGACAGGGCG
GCGACAATGTCGAGGAGGGCGAGGCTGTGGACGCTCTTGAAGCGCAGGTC
GGGTGCTCGCGCGGAGCCCCTTCTGGCGAGCGTGGACACGAGCATCATGT
CGCTGACGCCGTCCGAGCTTCTGTCGCCACCGGCAGAAGCAGAAGAGACC
GGCGGTTCCTTACTGTCATCTTCGAGCGGCGGGGGGGCCGAGGAGTCGGT
AGGTGGGGGGGGGGGGGGGGGC