mRNA_E-fasciculatus_F_contig138.2799.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

BLAST of mRNA_E-fasciculatus_F_contig138.2799.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 1795 bits (4649), Expect = 0.000e+0
Identity = 921/1021 (90.21%), Postives = 959/1021 (93.93%), Query Frame = 1

Query:  166 MLVASSLSAVLGVDLGKIQTITASSAFDDFARSQSAYSAASRWHIERLSHDAADGSRAPYVACADYKQGRHALTSLETAFAKSSVHRVSNTEADGSCFIVTTSPSSAAAMIRAPEDFALLSAAPLLPSLKLATSLLDHEPDTSPHLGATDDFGFSDHSVPLQSTYGERVTLDNVHGLSVRLSPGVLTAGGGESFAEAFLRDWHADLTSKSVHMRRAIFWSDSEADRSEYDVTRVREWSRAAAVVDGLASKRARTVGEICGFGKLRLRHVDDDLVLVEGMNHLLPGKEAPSELRTACYMGLLSQLAAKPEVLRVSPFQSASLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCYFADDDGLQIEHGFMFDGVRVEDDGNVSTVLGNYSFPYDMSRRKVVQYIQMFKTPDQGSISNGQDSFTYVSDYLGDPYHYDDSEYNSYDDYIGEYFSDTWSEESFCAEVLDDLSYHFSFLFDDTSYSSSSTSSSFSFTGGFHKDTVGGHGTWTAGSAAGAISSQSNLEEEACYGDELPGCAGGCISSSDVDAMLDNSIFDLDVFCPMYTCDGNTDIPSSECLSDDPVETLHQHGGVAPGAQISVFDASYTGTEIFASFAGNILWYSAMETGAKVHTNSWSSSTLCELTEDEYLYDTFMYENPEHLLIFAVGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPSRGTLTGDDGNLVYEELGLTDYSPEGYPWICLVPFLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCELLAGTGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMLINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEETIGSDGETTELVTVDGGAGVELRATLTWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTGSIYSSK 3228
            MLVASS S VLGVDLGKIQ ITASSAFDDFARSQSAYSAAS W+ +RLS+DAA+GS APYVACADYKQGRHALTSLETAF KS+VHRVSNTEADG+CFIVT  PSSA A+IRAPEDF LLSAAPLLPSLKLATSLLDH PDTSP LGA DDF  SDHS PL+STYGE +TLDNVHGLSVRLSPGVLTAGG ESFAEAFLRDWHADLTS+SV+MRRA FWSDS+ADRSEYDVTRVREWSRAAAVVDGLASK ARTVGEICGFGKL+LRHV DDLVLVEGMNHLLPGK  PSE RTACYMGLLSQLAAKPEVLRVSP QSA LHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSC+FADDDGLQ+EHGF+FDGVRVEDDGNVSTVLGNYSFP+DMSRRK+VQYIQ+FKTPDQGS S+GQDSFTYVSDYLGDPY YDDSEY SYDDY GEY+SDTWSE+SFCA VLDDLSYHFS+LFDDTSYSSSSTS S  FTGGFHKDTVGGHGTWTAGSAAGAIS+QS LEEEACYGDELPGCAGGCIS+SDVDAMLD+S FDLDVFCPMYTCDGNTDIPSSECLSDDPVE LHQHGGVAPGAQISVFD SY GTEI+ASFAGN LWYSAMETGAK+HTNSW  +TLC+LTEDEYLYDTFMYENPEHLLIFA GNDGGYEDIPNREACT+GSPALGKNSLAVGATSSGP  GT TGDDGNLVYEELGLTDYSPEGYPWICL PFLGTPSASTEPAGVDTVA+FSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSC+L+A  GTSASCPLVAGAAALVRQYFKDPSFFAKDV SRGGCAEHSSS QCSEFAPSAATVKAM+INSAHLMGGSSEPNG+RGFGRVHLEAGMPMDGVGETGL VVE  I +DGE TELVTVDG AGVELRATL WLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESG DDTVNVVERVIVPVESMESGVWSVVVSAK LLTDEQ YSLVVTG+IYSSK
Sbjct:    1 MLVASS-SPVLGVDLGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPS--FTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAI-ADGEITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAIYSSK 1017          

BLAST of mRNA_E-fasciculatus_F_contig138.2799.1 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 1635 bits (4235), Expect = 0.000e+0
Identity = 856/1036 (82.63%), Postives = 901/1036 (86.97%), Query Frame = 1

Query:  133 MGLRADHVAAFMLVASSLSAVLGVDLGKIQTITASSAFDDFARSQSAYSAASRWHIERLSHDAADGSRAPYVACADYKQGRHALTSLETAFAKSSVHRVSNTEADGSCFIVTTSPSSAAAMIRAPEDFALLSAAPLLPSLKLATSLLDHEPDTSPHLGATDDFGFSDHSVPLQSTYGERVTLDNVHGLSVRLSPGVLTAGGGESFAEAFLRDWHADLTSKSVHMRRAIFWSDSEADRSEYDVTRVREWSRAAAVVDGLASKRARTVGEICGFGKLRLRHVDDDLVLVEGMNHLLPGKEAPSELRTACYMGLLSQLAAKPEVLRVSPFQSASLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCYFADDDGLQIEHGFMFDGVRVEDDGNVSTVLGNYSFPYDMSRRKVVQYIQMFKTPDQGSISNGQDSFTYVSDY----LGDPYHYDDSEYNSYDDYIGEYFSDTWSEESFCAEVLDDLSYHFSFLFDDTSYSSSSTSSSFSFTGGFHKDTVGGHGTWTAGSAAGAISSQSNLEEEACYGDELPGCAGGCISSSDVDAMLDNSIFDLDVFCPMYTCDGNTDIPSSECLSDDPVETLHQHGGVAPGAQISVFDASYTGTEIFASFAGNILWYSAMETGAKVHTNSWSSSTLCELTEDEYLYDTFMYENPEHLLIFAVGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPSRGTLTGDDGNLVYEELGLTDYSPEGYPWICLVPFLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCELLAGTGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMLINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEETIGSDGETTELVTVDGGAGVELRATLTWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTGSIYSSK 3228
            MGLRADHVAA +LVAS  SAV+GVDLGKIQTITASSAFDDFARSQSAYSAAS+WH +RL+H A +GSRAPY+ACADYKQGRHA+TSLETAF +S+VHRVSNTE DGSCFIVT  PSSA AMI APEDFALLSAAP LPSLKLAT LLDH PDTSPH+GA DDF  SD S PL+STYGE                                                         DRS+YDVTRVREWSRAAAVVD LASKRA+T GEICGFGKL LRHV DDL+LVEGMNHLLPGK+APSE RTACYMGLLSQLAAKPEVLRVSP QSASL+NAVANALVQSATTTETPLLDAGLDGTGEVIQVVD+GLDETSC+FADDDGLQ+EHGF+F GVRVE DGNVSTVLGNYSFP+DMSRRK+VQYIQMFKTPDQGS S+GQ+SFTY SDY    LGD +  DDSEYNSYDDY GEY SDTWSEESFCA+VLDDLSYHFS LFDDTSYS SS SSS S TGGF KD VGGHGTWTAG AAGAIS+QS+L+EEACYGDELPGCAGGCIS+SDVDAMLDN  FDLDVFCPMY CDGNTDIPSSECLSDDPVE LHQHGGVAPGAQIS+ DASYTG++ FA FAGN+LWYSAMETGAK+H+NSW    LC+LTE+EYLYD+FMYENPEHLLIFA GN GG EDIPN E CTIGSPALGKN LAVGATSSGPSRGT TG+DGNLVYEE G+TDYSPEGYPWICL P LGTPSAS EPAGVDTVA+FSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSC LLA  GTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAM+INSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVE TIGSD E TELVTVDG AG ELRATL WLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTG+IYSSK
Sbjct:    1 MGLRADHVAAILLVASP-SAVIGVDLGKIQTITASSAFDDFARSQSAYSAASKWHTKRLAH-APEGSRAPYIACADYKQGRHAMTSLETAFTESAVHRVSNTETDGSCFIVTALPSSATAMISAPEDFALLSAAPFLPSLKLATRLLDHGPDTSPHVGAADDFNLSDLSAPLRSTYGE---------------------------------------------------------DRSQYDVTRVREWSRAAAVVDSLASKRAQTFGEICGFGKLSLRHVADDLLLVEGMNHLLPGKKAPSEARTACYMGLLSQLAAKPEVLRVSPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQVEHGFLFGGVRVEGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQNSFTYASDYIRDYLGDAFDDDDSEYNSYDDYSGEYSSDTWSEESFCAQVLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGSDDEITELVTVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTGAIYSSK 977          

BLAST of mRNA_E-fasciculatus_F_contig138.2799.1 vs. uniprot
Match: A0A6H5JQ52_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQ52_9PHAE)

HSP 1 Score: 986 bits (2549), Expect = 0.000e+0
Identity = 525/755 (69.54%), Postives = 575/755 (76.16%), Query Frame = 1

Query:  463 VTTSPSSAAAMIRAPEDFALLSAAPLLPSLKLATSLLDHEPDTSPHLGATDDFGFSDHSVPLQSTYGERVTLDNVHGLSVRLSPGVLTAGGGESFAEAFLRDWHADLTSKSVHMRRAIFWSDSEADRSEYDVTRVREWSRAAAVVDGLASKRARTVGEICGFGKLRLRHVDDDLVLVEGMNHLLPGKEAPSELRTACYMGLLSQLAAKPEVLRVSPFQSASLHNAVANALVQSATTTE---TPLLDAGLDGTGEVIQVVDSGLDETSCYFADDDGLQIEHGFMFDGVRVEDDGNVSTVLGNYSFPYDMSRRKV-------------------------------------------------VQYIQMFKTPDQGSISNGQDSFTYVSDYLGDPYHYDDSEYNSYDDYIGEYFSDTWSEESFCAEVLDDLSYHFSFLFDDTSYSSSSTSSSFSFTGGFHKDTVGGHGTWTAGSAAGAISSQSNLEEEACYGDELPGCAGGCISSSDVDAMLDNSIFDLDVFCPMYTCDGNTDIPSSECLSDDPVETLHQHGGVAPGAQISVFDASYTGTEIFASFAGNILWYSAMETGAKVHTNSWSSSTLCELTEDEYLYDTFMYENPEHLLIFAVGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPSRGTLTGDDGNLVYEELGLTDYSPEGYPWICLVPFLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDGHSCEL 2568
            V   PSSA A+IRAPEDF LLS  PLL SLKLATSLLDH PDTSP LGA D+   SDHS PL+STYGE                                           V+MRRA FWSDS+ADRSEYDVTRVREWSRAAAVVDGLASK ARTVGEICGFGKL+LRHV DDLVLVEGM +LLPGK  PSE RTACYMGLLSQLAAKPEVL VSP QSA+LHNAVANA +QSAT  +    P    G           DSGLD+TSC+FADDDGLQ+EHG++FDGVRVEDDGNVST LGNYSFP+DMSRRKV                                                 VQ IQ+FKTP QGS S+GQDSFT+ SD++GDP+ YDDSEY SY DY GEY+SDTWSE+SFCA  LDDLSYH S+L+DDTSY+S STS S  FTGGFH+DT+GGHGT T+GSAAGAISSQS+L+EEACYGDELPGCAGGCIS+SDVDAMLD+S F L VFCPMYTCDGN DIPSSECLSDDPVE LHQHGGVAPGAQISVFD SY GTE++AS+AGN LWYSAMETGAK+HTNSW  +TLC+LTE EY+YDTFMYENPEHLLIF+ GNDGGY+DIPNREACT+GSPALGKN LAVGATSSGP  GT TGDDGNL  EE+G+TDYSPEGYPWICL+P LG P ASTEPAGVDTV YFSSQGPTLDG+IKP VVAPG+QV ASASSDGTD HSC+L
Sbjct:    5 VAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGE------------------------------------------GVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPS--FTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 711          

BLAST of mRNA_E-fasciculatus_F_contig138.2799.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 657 bits (1695), Expect = 3.650e-214
Identity = 437/1026 (42.59%), Postives = 574/1026 (55.95%), Query Frame = 1

Query:  232 ASSAFDDFARSQSAYSAASRWHIERLSHDAADGSRAPYVACADYKQGRHALTSLETAFAKSSVHRVSNTEADGSCFIVTTSPSSAAAMIRAPEDFALLSAAPLLPSLKLATSLLDH----EPDTSPHLGATDDFGFSDHSVPLQSTYGERVTLDNVHGLSVRLSPGVLTAGGGESFAEAFLRDWHADLTSKSVHMRRAIFWSDSEADRSEY-----DVTRVREWSRAAAVVDGLASKRARTVGEICGFGKLRLRHVDDDLVLVEGMNHLL--------PGKEAPSELRTACYMGLLSQLAAKPEVLRVSPFQSASLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCYFADDDGLQIEHGFMFDGVRVEDDGNVST----VLGNYSFPYDMSRRKVVQYIQMFKTPDQGSISNGQDSFTYVSDYLGDPYHYDDSEYNSYDDYIGEYFSDTWSEESFCAEVLDDLSYHFSFLFDDTSYSSSSTSSSFSFTGGFHKDTVGGHGTWTAGSAAGAISSQSNLEEEACYGDELPGCAGGCISSSD-----VDAMLDNSIFDLDVFCPMYTCDGNTDIPSSECLSDDPVETLHQHGGVAPGAQISVFDASYTGTEIFA--SFAGNILWYSAMETGAKVHTNSWSSSTLCELTEDEYLYDTFMYENPEHLLIFAVGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPSRGTLTGDDGNLVYEELGLTDYSPEGYPWICLVPFLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCELLAGTGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMLINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLV---VEETIGSDGETTELVTVDGGAGVELRATLTWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTGSI 3216
            A++A DDF     A+ AA+ W+ ERL     DG+RAP++ CA+Y  GR   + L+   +  +V  VS++E  G+CF+ T S + A+A++   E F L S +P   +LKLA  L+DH    E +  P  G             L + +G  + + NV GL+V+L+P  L A      AE+F+ D   DL S SV +    FWSD      E+        R R+WS+AA+VV  L+     + G+IC + ++ L    DD +LV G++HLL          +E  +EL  AC+MGL+S  A +PEVLRVSP  +  + N  A  ++QSA+ T+TPL DAGLDGTGEVIQVVD+GLDETSC+F DD G +++HG+ F+ + V   G+       V     F Y   RRKV+QYI++   PD              SDY+                     FS T                 + FL  D                 F +D   GHGT TAGSAAGA  +    E   C GDE  GC GGCI  S+     V   +  ++ DLD  CPMY CDG+TD     CLSD+  ETL ++GG+A GA++S+FD  +   E+F      GN +W   +E G KVH+ S    TLC   E E LYD FMYENPE+LLIFA GNDG  E    R  CT+ SPA+GKN LAVGA++SG +R         ++  E G                           AG+D VA FSS G T DGRIKP+VVAPGD V SA+SDGTD HSC L A  GTS SCP+VAGAAA+VRQYF D +F+  DVT+RG C++      C  F+PSAATVKA+LINSA+LMG SS+P+  RGFGR+HLE GMP++G G+  L V   ++ +I  D     L  VD  AG+ELRATL+W+D P T+ SA QL +DLDL V++PSG  Y MW S   D  NV ERVIV  + +E+G W+V+V +  L+TD QSYSLVV G+I
Sbjct:   42 ATTAKDDFNGHARAFEAAATWNGERLRRSQ-DGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQD----------RLGARHGGTMRMSNVEGLNVKLTPDTLPARSAG--AESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQLIYYPVFEAGDFTYTEERRKVIQYIELVN-PD--------------SDYVA--------------------FSTTQGGV-------------YPFLPPDE----------------FGQDDSAGHGTHTAGSAAGATLNDP-AETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTD----PCLSDEVDETLTKNGGMAQGAKLSIFDVFF---EVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE----RSVCTMASPAIGKNVLAVGASTSGDTR---------VISTEGG---------------------------AGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSD---GFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAI 939          

BLAST of mRNA_E-fasciculatus_F_contig138.2799.1 vs. uniprot
Match: A0A6H5KDF8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDF8_9PHAE)

HSP 1 Score: 631 bits (1627), Expect = 7.280e-211
Identity = 333/491 (67.82%), Postives = 361/491 (73.52%), Query Frame = 1

Query: 1357 YSFPYDMSRRKVVQYIQMF---KTPDQGSISNGQDSFTYVSDYLGDPYHYDDSEYNSYDDYIGEYFSDT-------------------------------------------------WS---------EESFCAEV--------------------------LDDLSYHFSFLFDDTSYSSSSTSSSFSFTGGFHKDTVGGHGTWTAGSAAGAISSQSNLEEEACYGDELPGCAGGCISSSDVDAMLDNSIFDLDVFCPMYTCDGNTDIPSSECLSDDPVETLHQHGGVAPGAQISVFDASYTGTEIFASFAGNILWYSAMETGAKVHTNSWSSSTLCELTEDEYLYDTFMYENPEHLLIFAVGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPSRGTLTGDDGNLVYEELGLTDYSPEGYPWICLVPFLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCEL 2568
            +S PY  +   VV Y  +    KTPDQGS S+GQDSFTY SDY GDPY YDDSEY S+DDY GEYFSDT                                                 W          EE    E+                          LDDL+YH S+L+DDTSY+SSSTS S  FTGGFHKDT+GGHGT TAGSAAGAISSQS+LEEEACYGDELP CAGGCIS+SDV+AMLD+S FDLDVFCPMYTCDGNTDIPSSECLSDDPVE LHQHGG APGAQISVFD SY GTE++AS+AGN LWYSAMETGAK+HTNSW  +TLC+LTE EY+YD FMYENPEHLLIF+ GNDGGYEDIPNREACT+GSPALGKNSLAVGATSSGP  GT TGDDGNL YEE+GLTDYSPEGYPWICL+P+LGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTD HSC+L
Sbjct:   15 FSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPS--FTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 503          

BLAST of mRNA_E-fasciculatus_F_contig138.2799.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 628 bits (1619), Expect = 3.070e-203
Identity = 419/1024 (40.92%), Postives = 555/1024 (54.20%), Query Frame = 1

Query:  217 IQTITASSAFDDFARSQSAYSAASRWHIERLSHDAADGSRAPYVACADYKQGRHALTSLETAFAKSSVHRVSNTEADGSCFIVTTSPSSAAAMIRAPEDFALLSAAPLLPSLKLATSLLDHEPDTSPHLGATDDFGFSDHSVPLQSTYGERVTLDNVHGLSVRLSPGVLTAGGGESFAEAFLRDWHADLTSKSVHMRRAIFWSDSEAD------RSEYDVTRVREWSRAAAVVDGLASKRARTVGEICGFGKLRLRHVDDDLVLVEGMNHLL--------PGKEAPSELRTACYMGLLSQLAAKPEVLRVSPFQSASLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCYFADDDGLQIEHGFMFDGVRVEDDGNVSTVLGNYSFPYDMSRRKVVQYIQMFKTPDQGSISNGQDSFTYVSDYLGDPYHYDDSEYNSYDDYIGEYFSDTWSEESFCAEVLDDLSYHFSFLFDDTSYSSSSTSSSFSFTGGFHKDTVGGHGTWTAGSAAGAISSQSNLEEEACYGDELPGCAGGCISSSD-------VDAMLDNSIFDLDVFCPMYTCDGNTDIPSSECLSDDPVETLHQHGGVAPGAQISVFDASYTGTEIFASFAGNILWYSAMETGAKVHTNSWSSSTLCELTEDEYLYDTFMYENPEHLLIFAVGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPSRGTLTGDDGNLVYEELGLTDYSPEGYPWICLVPFLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCELLAGTGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMLINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVE--ETIGSDGETTE-LVTVDGGAGVELRATLTWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTGSI 3216
            ++    ++A DDF     A+ AA+ W+ ERL H   + +RAP++ACA Y  G  A + L+   +  +V  VS++E  G+CF  + S + AAA++   E F L + +P    LKLA  L+DH   T  H  A ++   S     L + +G R+   NV GLSV L+PG L A   E  A++F+     DL S SV +    FWSD   D       S     R R+W +AA+VV  L+   +   G+IC + ++ + H  DD +LV G++HLL          +E  +EL  AC+MGL+S LA +PEVLRVSP  +  + NA A  ++QSA+ T+TPL DAGLDGTGE+IQV+DSGLDETSCYF DD G +++HG+ FD                     ++ R  V  Y   + + +  +I NG D F+Y                                                                       + +    GHGT TAGSAAGA  +    E   C   +   C G CI   D       + +  D +  DLD  CPMY CD +TD   + CLSDD  ETL  +GG+A GA++++FD  Y G +  +S  GN LW    E G K+H+NS  +   C +   + L D FMYEN E+LLIFA GN+GG E    R  CTI +P +GKNSLA+GA++SG +R          V   +G T                          G+DTVA FSS G T D RIKP+VVAPGD V SA+SDGTD HSC L A  GTS SCP+VAGAAA++RQYF D +F+  DVT+RG C++      C  F+PSAATVKA+LINSA+LMGGSSEP+G RGFGRVHLEAGMP++G G+  L V +  +T  + G   E L  VD  AG++LRATL+W+DPP TA SA QL H+LDL V++P G  Y MW S   D  NV ERVIV    + +G W+V V A  L+TD QSYSLVV G+I
Sbjct:   32 VENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEE-TRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDH---TESHDEAEEE-ARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSE--AKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFD---------------------ELGRAAV--YSSSYSSDETATIFNGGD-FSY-----------------------------------------------------------------------YPERRKAGHGTHTAGSAAGATLNDP-AETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGT--DLDRLCPMYGCDNSTD---TTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSI-GNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE----RSTCTINTPGIGKNSLAIGASTSGDTR----------VISTVGAT--------------------------GIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSD---GFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAI 903          

BLAST of mRNA_E-fasciculatus_F_contig138.2799.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 610 bits (1573), Expect = 3.560e-195
Identity = 422/1056 (39.96%), Postives = 571/1056 (54.07%), Query Frame = 1

Query:  217 IQTITASSAFDDFARSQSAYSAASRWHIERLSHDAADGSRAPYVACADYKQGRHALTSLETAFAKSSVHRVSNTEADGSCFIVTTSPSSAAAMIRAPEDFALLSAAPLLPSLKLATSLLDHEPDTSPHLGATDDFGFSDHSVPLQSTYGERVTLDNVHGLSVRLSPGVLTAGGGESFAEAFLRDWHADLTSKSVHMRRAIFWSDSEADRSEY-----DVTRVREWSRAAAVVDGLASKRARTVGEICGFGKLRLRHVDDDLVLVEGMNHLL-PGKEA--------PSELRTACYMGLLSQLAAKPEVLRVSPFQSASLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCYFA-DDDGLQIEHGFMFD--GV-----------------------RVEDD---GNVSTVLGNYS---FPYDMSRRKVVQYIQMFKTPDQGSISNGQDSFTYVSDYLGDPYHYDDSEYNSYDDYIGEYFSDTWSEESFCAEVLDDLSYHFSFLFDDTSYSSSSTSSSFSFTGGFHKDTVGGHGTWTAGSAAGAISSQSNLEEEACYGDELPGCAGGCIS---SSDVD--AMLDNSIFDLDVFCPMYTCDGNTDIPSSE-CLSDDPVETLHQHGGVAPGAQISVFDASYTGTEIFASFAGNILWYSAMETGAKVHTNSWSSSTLCELTEDEYLYDTFMYENPEHLLIFAVGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPSRGTLTGDDGNLVYEELGLTDYSPEGYPWICLVPFLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGT-DGHSCELLAGTGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMLINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEETIGSDGETTEL---VTVDGGAGVELRATLTWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTGSI 3216
            ++   +++A DDF     A+ AA+ W+ ERL H   D +RAP++ CA+Y  GR   + L+   +  +V  VS++E  G+CF  + S + A+A+I   E F L S +P   +LKLA  L+DH          T           L + +G R+ + NV GLS+ L+PG L +   E  AEAF+ D   DL   SV +    FWSD      E         R R+WS+AA+VV  L+     + G+IC + ++ + H + D++LV G++HLL  G+ A         +EL  AC+MGL+S LA+KPEVLRV+P QS +L NA A A +QSAT T+TPL DAGLDGTG+V+QV+DSG+DE+SC+FA D+ G  + HG  F+  G+                       R  DD   G+   +   +    F     RRK++QYI + K+ D    S+G DSFT     LG  Y                     W                                        F  D   GHGT  AGS AGA  + +  E  +C   E   C G C+    ++DVD  A  +  I DLD  CP+++ D + D+  +E CLSDD  ETL  +GG+A GA++++FDA Y    + + F GN LW    E   K+H+NS+     C L  ++ LYD +M  NPE+LLIFA GNDG       R  CT+ SPA+ KN LAVGA++SG +R                                      ++T   G+DTVA FSS GPT D RIKP+V+APGD + SA+SDG+ D HSC L A  GTS S P+VAGA+A++RQYF + +FFA D+T+RG C E +    C  F+PS+AT+KA++INSA+LMGGSSEP+G RGFGR+HLEAGMP+ G G   L V +    S  E T       V+  A ++LR TL+WLDPP T  SA QL HDLDL VI+P+G +YTMW SG  D+ NV ERVIV    +E+G WSV + A  L TD QSYSLVV G+I
Sbjct:   27 VENALSTTAKDDFNGHARAFEAAATWNGERLRHRQ-DETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLD------RLSARHGARMRMSNVEGLSIELTPGTLASRSVE--AEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEG-SHG-DSFTTS---LGQQY--------------------PWLPAD-----------------------------------RFEYDYEAGHGTHVAGSVAGATLN-TPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFS-DYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGL-SPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERR----RSTCTMSSPAIAKNVLAVGASTSGDTR-------------------------------------LSATPGLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG---CEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAI 966          

BLAST of mRNA_E-fasciculatus_F_contig138.2799.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 576 bits (1484), Expect = 1.740e-184
Identity = 395/1009 (39.15%), Postives = 527/1009 (52.23%), Query Frame = 1

Query:  196 LGVDLGKIQTITASSAFDDFARSQSAYSAASRWHIERLSHDAADGSRAPYVACADYKQGRHALTSLETAFAKSSVHRVSNTEADGSCFIVTTSPSSAAAMIRAPEDFALLSAAPLLPSLKLATSLLDHEPDTSPHLGATDDFGFSDHSVPLQSTYGERVTLDNVHGLSVRLSPGVLTAGGGESFAEAFLRDWHADLTSKSVHMRRAIFWSDSEADRSEYDVT-----RVREWSRAAAVVDGLASKRARTVGEICGFGKLRLRHVDDDLVLVEGMNHLLPGK-------EAPSELRTACYMGLLSQLAAKPEVLRVSPFQSASLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCYFADDDGLQIEHGFMFDGVRVEDDGNVSTVLGNYSFPYDMSRRKVVQYIQMFKTPDQGSISNGQDSFTYVSDYLGDPYHYDDSEYNSYDDYIGEYFSDTWSEESFCAEVLDDLSYHFSFLFDDTSYSSSSTSSSFSFTGGFHKDTVGGHGTWTAGSAAGAISSQSNLEEEACYGDELPGCAGGCISSSDVD-------AMLDNSIFDLDV--FCPMYTCDGNTDIPSSECLSDDPVETLHQHGGVAPGAQISVFDASYTGTEIFASFAGNILWYSAMETGAKVHTNSWSSSTLCELTEDEYLYDTFMYENPEHLLIFAVGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPSRGTLTGDDGNLVYEELGLTDYSPEGYPWICLVPFLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCELLAGTGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMLINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEETIGSDGETTE---LVTVDGGAGVELRATLTWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWE-SGADDTVNVVERVIVPVESMESGVWS 3147
            L V  G + T TA  +FD  A+   A+  A++W  E L     D +RAP++ C +Y +G   L  L+   +  +V  V+++   G+CF VT S + A AM  +P  + L S +P   +LK+A  +L+H        G + D   S     L +T+G  +   NV GL++ LSPG L A   E  A  F+ +   DL S S+ +     WSD  A   E+  T     R REWS AA +V  LA     + G+IC +  + + +  +DL+LV G++HLL  +       E  +ELR AC+MGL+S L+ + EVLRVSP  +  L NAVA  ++QSAT TE PL DAGLDGTG+VIQV+D+GLDETSC+F DDDG ++ HG  ++ +                                                                                        E++                SSSS            +D V GHGT  AGSA GA           C G E+PGC GGCI +            M + S +D+D+   CPM  CD  TD     CLSDD  +TL  HGG+A GA+++ FD    G+     F GN LW   +E G K+H+ S  +   C + E + LYD FMY NPE+L++ A GN G  ED   R  CT+ SPA+GKN+LAVGATSSG +R T T  DG+L+    G  D                          VDTVA FSS GPT DGRIKP+VVAPGD + SA SDG+D HSC L A +GTS +CP+VAGA+A+VRQYF D SF+  D  +RG   +  S  +C  F+PS+ATVKA+LINSA+LMGGSSEP+G RGFGR+HLE GMP+DG G   L V + +  S  E      +  VD  A ++ RATL+W+DPP ++ S  QL HDLDL V++PSGA + MW  SG  D+ NV ERVI+  E +ESG ++
Sbjct:   34 LSVVEGSLLT-TAQDSFDGHAK---AFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDSPY-YGLTSISPFPSALKVAPGVLEH--------GVSLDQA-SQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPE--AGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEAL---------------------------------------------------------------------------------------GEIIP---------------SSSSA-----------RDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATD---PRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSS-GTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTED--GRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYAD--------------------------VDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDD--SGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTYT 879          

BLAST of mRNA_E-fasciculatus_F_contig138.2799.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 579 bits (1492), Expect = 1.840e-182
Identity = 435/1119 (38.87%), Postives = 569/1119 (50.85%), Query Frame = 1

Query:  259 RSQSAYSAASRWHIERLSHDAADGSRAPYVACA--DYKQGRHALTSLETAFAKSSVHRVSNTEAD-GSCFIVTTSPSSAAAMIRAPEDFALLSAAPLLPSLKLATSLLDHEPDTSPHLGATDDFGFSDHSVPLQSTYGERVT---------------------------------------LDN-------VHGLSVRLSPGVLTA-------GGGESFAEAFLRD-WHADLTSKSVHMRRAIFWS--------DSEADRSEYDVTR-------VREWSRAAAVVDGLA-SKRARTVGEICGFGKLRLRHVDDDLVLVEGMNHLLP------GKEAPS--ELRTACYMGLLSQLAAKPEVLRVSPFQSASLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCYFADDDGLQ-IEHGFMFDGVRVEDDG------------NVSTVLGNYSFPYDMSRRKVVQYIQMFKTPDQGSISNGQDSFTYVSDYLGDPYHYDDSEYNSYDDYIGEYFSDTWSEESFCAEVLDDLSYHFSFLFDDTSYSSSSTSSSFSFTGGFHKDTVGGHGTWTAGSAAGAISSQSNLEE-----EACYGDELPGCAGGCIS------SSDVDAMLDNSIF-------DLDVFCPMYTCDGNTDIPSSECLSDDPVETLHQHGGVAPGAQISVFDA--SYTGTEIFASFAGNILWYSAMETGAKVHTNSWSSSTLCELTEDEYLYDTFMYENPEHLLIFAVGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPSRGTLTGDDGNLVYEELGLTDYSPEGYPWICLVPFLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCELLAGTGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMLINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEETIGSDGETTEL---VTVDGGAGVELRATLTWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESM---------------ESGVWSVVVSAKGLLTDE-QSYSLVVTGSI 3216
            R Q  + AA  WH  RL     D   APY+ACA  D  +G    T      A +   RV  + A+ G C + T +P  A ++ +  + F+  +A P   +LKLA  LLDH     PH    DD         L+ T+GER+                                         DN       V GL+V L+PGVL A       GGG   +   L D W  +L S+S+ +    FWS        D  A     D +         REW+RAA V+ GL  ++   T+G++C +  L + H DDDL+ ++GM+HLLP      G E P   EL+ AC+M LLS LA++PEVLRVSP ++  L NA A   VQSA+ TE+P+ DAGLDGTGEVIQ+VD+GLD  SC+F D+DG + + HG+ F+ + VE                ++ V     F +D+SRRK +       + D  +IS     F      LGD                       WS E+      +  +Y  S+       +S+     +     F +D  GGHGT TAGSAAGA+ S  +  +     E C       C GGCI         D+    D   F       DLD  CP   CD   D+P   CL DD  ETL QHGGVA GAQ+++FD    YTG  I    AGN +W  AME G+K+ +NSW     C+    + L+D FMY N + LL+FA GNDG      +   CT+G+PAL KN LAVGA++SGP R + T +DG+    +  + D                          +D +AYFSS GPTLD RIKP+VVAPGD + SA+  G   ++C L    GTS S P+VAGAAALVRQYFK+ +F+A DV  RG C     S  C  FAPSAATVKAMLINSA+LMGGS+EP+GLRGFGRVHLEAGMP+ G G+  L V +  + S  E T       VD  AG++LRATL+W+DPP T  S+ QL HDLDL V +P+G +Y+MW  G  D++N  ERVIVP  ++                 G W+V V +K L  +E QSYSLVVTG+I
Sbjct:   48 RHQGEFKAAVEWHEARLD----DARPAPYLACASPDSDRGGSGATLSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQVFSSWAAFPS--ALKLAPGLLDH-----PH----DDRDGGGGDGRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISR---RFA-----LGDVR--------------------AWSGENPACTANNSTNYGESWA------TSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD---DLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGI--DMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGD-----DWSGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQD--------------------------IDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFC----DSFGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAI 1077          

BLAST of mRNA_E-fasciculatus_F_contig138.2799.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 569 bits (1467), Expect = 2.030e-180
Identity = 394/1029 (38.29%), Postives = 542/1029 (52.67%), Query Frame = 1

Query:  229 TASSAFDDFARSQSAYSAASRWHIERLSHDAADGSRAPYVACADYKQGRHALTSLETAFAKSSVHRVSNTEADGSCFIVTTSPSSAAAMIRAPEDFALLSAAPLLPSLKLATSLLDHEPDTSPHLGATDDFGFSDHSVP-LQSTYGERVTLDNVHGLSVRLSPGVLTA-GGGESFAEAFLRDWHADLTSKSVHMRRAIFWSDSEADRSEY-----DVTRVREWSRAAAVVDGLASKRARTVGEICGFGKLRLRHVDDDLVLVEGMNHLL--------PGKEAPSELRTACYMGLLSQLAAKPEVLRVSPFQSASLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCYFADDDGLQIEHGFMFDGVRV---------EDDGNVSTVLGNYSFPYDMSRRKVVQYIQMFKTPDQGSISNGQDSFTYVSDYLGDPYHYDDSEYNSYDDYIGEYFSDTWSEESFCAEVLDDLSYHFSFLFDDTSYSSSSTSSSFSFTGGFHKDTVGGHGTWTAGSAAGA-ISSQSNLEEEACYGDELPGCAGGCISSSDVDAMLDNSIFDLDVFCPMYTCDGNTDIPSSECLSDDPVETLHQHGGVAPGAQISVFDASYTGTEIFASFAGNILWYSAMETGAKVHTNSWSSSTLCELTEDEYLYDTFMYENPEHLLIFAVGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPSRGTLTGDDGNLVYEELGLTDYSPEGYPWICLVPFLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCELLAGTGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMLINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEET---IGSDGETTELVTVDGGAGVELRATLTWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESME----SGVWSVVVSAKGLLT-DEQSYSLVVTGSI 3216
            TA + FD    S++A++ AS W           G  +PY++C ++ +GRHA   LE      +V  VS+T   G+CF+VT SP+ AA  + + EDF    A P   +LK+A  LLDH     P          SD S   L +T+G  + +D+ HGL V LSPG L +  GG++ A  F+++    L+S S+ +     WSD      ++        R REWSRAA +V+  +     T  ++CG+ ++ + H  +D++LV G++HLL          +E  +ELR ACYMGL+S LA++ EVL+V+ +      NA A A +QSA+   TPL DAGLDG+GEVIQ++D+G++ET+C+    DG ++EHG+ FD V           E             F YD  RRKV+QYI +   PD G  S+    F     ++ D                                  DDL                                  GHGT  A + AG+ ISS + L   A  G E+ GCAGGCI+ S   +   +   D++  CP + C G+      +CL DD  +TL +HGG+A GA+I+  D  Y G   +   AGN LW +  + G K+H+NS+     C+LT  + LYD +MYEN EHLL+F+ GN G   D   R+ CTI SP + KN L VG+TSSG +R              L  TD    GY            +   E + +D V+ FS  G T DGRIKP+VVAPGD + SAS +G D  SCEL+A  GTS S  + +GAAA+VRQYF+D SF++ DV SRG C +  +   C  F+P A TVKAMLINSA++MGG +EP+  RGFGR+HLEAGMP+ G G+  L V + +   + SD +      VD  AG+ELRATL+W DP  ++LS  QL +DLDL V AP G +Y MW+    DT NV ERVIVP E +     +  W V V  +     D  S+SLVVTG+I
Sbjct:   37 TAGADFD----SEAAFAFASAWQAGDR-----QGRSSPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAA-QLSSWEDFTTFGAFPA--ALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLIN-PDTGDGSSSGMPFVSADSFVSD----------------------------------DDL----------------------------------GHGTHAATTVAGSTISSPAELVSCA-DGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGD------QCLPDDTADTLSEHGGMAQGAKIAAIDVFY-GDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTD---RDTCTIDSPGIAKNVLTVGSTSSGVTR--------------LSTTDLDGRGY------------ADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGD-SSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFT---CDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAI 943