mRNA_E-fasciculatus_F_contig1356.2648.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig1356.2648.1
Unique NamemRNA_E-fasciculatus_F_contig1356.2648.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig1356.2648.1 vs. uniprot
Match: A0A6H5JHQ3_9PHAE (DNA polymerase lambda n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHQ3_9PHAE)

HSP 1 Score: 466 bits (1199), Expect = 5.380e-153
Identity = 269/376 (71.54%), Postives = 288/376 (76.60%), Query Frame = 1 
Query:  250 MASSPSIYHQGKHSLFTQRFRKIDAIRNALRTAVTEELAQQTQEKRRETYRVNAENKRRSA-AAAAALDTKTDTSGSLPGSNSVGDGHGPEVSIAVPCRLGCDGAARKKTRSASSSSFGNSGGRAVSRPHDRQGEPLSTAACVKIASADPDKAFGLRVDSGDAPRGEEEGASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVFTESQSAFASAGAKGGGGAKAGVRFFEDILRLGPKGTRPNAVGGALPNSKRKPHPRVRGGCKGEASRTQANKKPRQGTGAVPGPFAGLSLCVVHLGSVTSNTVKAFKRGVCDGGGQVATRFMPADTTHIVADPSLAASWEKLDNYFSGRGGIDDLDGTVGGGGVGLKRG 1374
            MASSPS+YHQGKH+ FTQRFRKIDAIR ALRTAV EELAQQT+E+RRET+RVNAENKRRSA AAAAALD KTD+SG  PG +S  DG+G EVSI+VPCRLGCDG A  +TRSA     G+SGGRAVSRP DR  E LSTAACVK ASA PDKAFG RVDSGD P GE+EGAS                                  V TESQS  ASAGAKGGG AK GVRF EDILRLGPKG+R N  GGALPN KRK HPR RGGCKGEASRTQ +KKPRQGTGAV GPF+GLSLCVVHLGSVT+NT+K FKRGVCDGGG V+ RF P DTTHIVAD SLAASWEKLDNYFSG GG+DDLD  V GG VGLK G
Sbjct:    1 MASSPSVYHQGKHNPFTQRFRKIDAIRTALRTAVAEELAQQTEERRRETHRVNAENKRRSATAAAAALDGKTDSSGIFPGRDS--DGYGSEVSISVPCRLGCDGTA-SRTRSA-----GSSGGRAVSRPRDRLVELLSTAACVKQASASPDKAFGWRVDSGDGPEGEQEGASGVASTCGGRRDGHDRGPTPPPAAAS---------VSTESQSVLASAGAKGGG-AKTGVRFLEDILRLGPKGSRSNDAGGALPNPKRKSHPR-RGGCKGEASRTQTHKKPRQGTGAVSGPFSGLSLCVVHLGSVTNNTIKTFKRGVCDGGGHVSMRFTPGDTTHIVADASLAASWEKLDNYFSGWGGVDDLDDAVNGGRVGLKSG 357
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BLAST of mRNA_E-fasciculatus_F_contig1356.2648.1 vs. uniprot
Match: D7G2F7_ECTSI (DNA polymerase lambda n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G2F7_ECTSI)

HSP 1 Score: 142 bits (358), Expect = 8.260e-33
Identity = 75/133 (56.39%), Postives = 91/133 (68.42%), Query Frame = 1 
Query:  934 VRFFEDILRLGPKGTRPNAVGGALPNSKRKPHPRVRGGCKGEASRTQANKKPRQGTGAVPGPFAGLSLCVVHLGSVTSNTVKAFKRGVCDGGGQVATRFMPADTTHIVADPSLAASWEKLDNYFSGRGGIDDL 1332
            +R+ ED+L   PKG+R NAV  A P+ KRK  PR  GG  G+    + NK+P +  GA  GPF+GLSLCVV  GSVT+NTV    R   DGGGQV+T + P DTTHIVAD SLAASWEKLD+YFSG    +D+
Sbjct:    3 IRYLEDLLCSAPKGSRSNAV--AAPSPKRKNRPR--GGASGDNGGIETNKRPWKRAGAASGPFSGLSLCVVSQGSVTNNTVGTLTREFRDGGGQVSTHYTPGDTTHIVADASLAASWEKLDDYFSGWDDFEDV 131
[Close]
BLAST of mRNA_E-fasciculatus_F_contig1356.2648.1 vs. uniprot
Match: A0A6H5JQ91_9PHAE (DNA polymerase lambda n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQ91_9PHAE)

HSP 1 Score: 140 bits (354), Expect = 2.540e-32
Identity = 76/133 (57.14%), Postives = 89/133 (66.92%), Query Frame = 1 
Query:  934 VRFFEDILRLGPKGTRPNAVGGALPNSKRKPHPRVRGGCKGEASRTQANKKPRQGTGAVPGPFAGLSLCVVHLGSVTSNTVKAFKRGVCDGGGQVATRFMPADTTHIVADPSLAASWEKLDNYFSGRGGIDDL 1332
            +R  ED+L  GPKG+R NAV  A PN KRK  PR  GG  G+    +  K+P +  GA  GPF+GLSLCVV LGSVT+NTV    R   DGGGQV+T + P DTTHIVAD SLAASWEK D YFSG    +D+
Sbjct:    3 IRDLEDLLCSGPKGSRSNAV--AAPNPKRKTRPR--GGAGGDNGGIKTKKRPWKRAGAASGPFSGLSLCVVRLGSVTNNTVGTLPREFRDGGGQVSTHYTPGDTTHIVADASLAASWEKFDLYFSGWDDFEDV 131
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig1356.2648.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
A0A6H5JHQ3_9PHAE5.380e-15371.54DNA polymerase lambda n=1 Tax=Ectocarpus sp. CCAP ... [more]
D7G2F7_ECTSI8.260e-3356.39DNA polymerase lambda n=1 Tax=Ectocarpus siliculos... [more]
A0A6H5JQ91_9PHAE2.540e-3257.14DNA polymerase lambda n=1 Tax=Ectocarpus sp. CCAP ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig1356contigE-fasciculatus_F_contig1356:10978..12351 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score141.7
Seed ortholog evalue6.8e-31
Seed eggNOG ortholog2880.D7G2F7
Preferred namePOLB
KEGG rclassRC00536
KEGG koko:K01834,ko:K02330,ko:K03512,ko:K10981
KEGG ReactionR01518
KEGG Pathwayko00010,ko00260,ko00680,ko01100,ko01110,ko01120,ko01130,ko01200,ko01230,ko03410,ko03450,ko04922,ko05166,ko05203,ko05230,map00010,map00260,map00680,map01100,map01110,map01120,map01130,map01200,map01230,map03410,map03450,map04922,map05166,map05203,map05230
KEGG ModuleM00001,M00002,M00003,M00296
GOsGO:0000228,GO:0000724,GO:0000725,GO:0000726,GO:0000793,GO:0000794,GO:0000795,GO:0002200,GO:0002376,GO:0002377,GO:0002440,GO:0002520,GO:0002562,GO:0002566,GO:0003674,GO:0003676,GO:0003677,GO:0003684,GO:0003824,GO:0003887,GO:0003906,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005694,GO:0005737,GO:0005819,GO:0005856,GO:0005874,GO:0005876,GO:0006139,GO:0006259,GO:0006260,GO:0006261,GO:0006266,GO:0006281,GO:0006284,GO:0006285,GO:0006286,GO:0006287,GO:0006288,GO:0006289,GO:0006290,GO:0006302,GO:0006303,GO:0006310,GO:0006464,GO:0006508,GO:0006725,GO:0006793,GO:0006796,GO:0006807,GO:0006915,GO:0006950,GO:0006952,GO:0006954,GO:0006974,GO:0007154,GO:0007165,GO:0007275,GO:0007431,GO:0007435,GO:0007568,GO:0008017,GO:0008092,GO:0008150,GO:0008152,GO:0008219,GO:0008630,GO:0009058,GO:0009059,GO:0009314,GO:0009411,GO:0009416,GO:0009628,GO:0009636,GO:0009653,GO:0009887,GO:0009987,GO:0010033,GO:0010212,GO:0010224,GO:0010332,GO:0012501,GO:0015630,GO:0015631,GO:0016311,GO:0016444,GO:0016445,GO:0016446,GO:0016447,GO:0016579,GO:0016740,GO:0016772,GO:0016779,GO:0016829,GO:0016835,GO:0018130,GO:0019438,GO:0019538,GO:0019899,GO:0022612,GO:0023052,GO:0030145,GO:0031974,GO:0031981,GO:0032501,GO:0032502,GO:0032991,GO:0033151,GO:0033152,GO:0033554,GO:0034061,GO:0034641,GO:0034645,GO:0034654,GO:0035272,GO:0035556,GO:0036211,GO:0036296,GO:0042221,GO:0042493,GO:0042592,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043412,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044430,GO:0044446,GO:0044454,GO:0044464,GO:0045471,GO:0046483,GO:0046677,GO:0046872,GO:0046914,GO:0048513,GO:0048534,GO:0048535,GO:0048536,GO:0048731,GO:0048732,GO:0048856,GO:0048872,GO:0050789,GO:0050794,GO:0050896,GO:0051103,GO:0051402,GO:0051575,GO:0051716,GO:0055093,GO:0065007,GO:0065008,GO:0070013,GO:0070482,GO:0070646,GO:0070647,GO:0070997,GO:0071704,GO:0071707,GO:0071897,GO:0090304,GO:0097159,GO:0097190,GO:0097193,GO:0097305,GO:0097510,GO:0098501,GO:0098502,GO:0099080,GO:0099081,GO:0099086,GO:0099512,GO:0099513,GO:0140097,GO:1901360,GO:1901362,GO:1901363,GO:1901564,GO:1901576,GO:1901700
EggNOG free text desc.DNA-directed DNA polymerase activity
EggNOG OGsCOG1796@1,KOG2534@2759
Ec32 ortholog descriptionDNA polymerase beta, thumb domain
Ec32 orthologEc-27_001410.2
EC2.7.7.7,5.4.2.11
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03400,ko04131,ko04147
Hectar predicted targeting categoryother localisation
Exons1
Model size1374
Cds size1125
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927853.1431408-UTR-E-fasciculatus_F_contig1356:10977..112261622927853.1431408-UTR-E-fasciculatus_F_contig1356:10977..11226Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig1356 10978..11226 +
1695995723.8535404-UTR-E-fasciculatus_F_contig1356:10977..112261695995723.8535404-UTR-E-fasciculatus_F_contig1356:10977..11226Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig1356 10978..11226 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927853.15953-CDS-E-fasciculatus_F_contig1356:11226..123511622927853.15953-CDS-E-fasciculatus_F_contig1356:11226..12351Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1356 11227..12351 +
1695995723.867747-CDS-E-fasciculatus_F_contig1356:11226..123511695995723.867747-CDS-E-fasciculatus_F_contig1356:11226..12351Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1356 11227..12351 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig1356.2648.1prot_E-fasciculatus_F_contig1356.2648.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig1356 11227..12351 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig1356.2648.1

>prot_E-fasciculatus_F_contig1356.2648.1 ID=prot_E-fasciculatus_F_contig1356.2648.1|Name=mRNA_E-fasciculatus_F_contig1356.2648.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=375bp
MASSPSIYHQGKHSLFTQRFRKIDAIRNALRTAVTEELAQQTQEKRRETY
RVNAENKRRSAAAAAALDTKTDTSGSLPGSNSVGDGHGPEVSIAVPCRLG
CDGAARKKTRSASSSSFGNSGGRAVSRPHDRQGEPLSTAACVKIASADPD
KAFGLRVDSGDAPRGEEEGASGAASTSGGRRDDDDDHGPTPPTAAAAAAA
AAAASVFTESQSAFASAGAKGGGGAKAGVRFFEDILRLGPKGTRPNAVGG
ALPNSKRKPHPRVRGGCKGEASRTQANKKPRQGTGAVPGPFAGLSLCVVH
LGSVTSNTVKAFKRGVCDGGGQVATRFMPADTTHIVADPSLAASWEKLDN
YFSGRGGIDDLDGTVGGGGVGLKRG
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mRNA from alignment at E-fasciculatus_F_contig1356:10978..12351+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig1356.2648.1 ID=mRNA_E-fasciculatus_F_contig1356.2648.1|Name=mRNA_E-fasciculatus_F_contig1356.2648.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=1374bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1356:10978..12351+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
CTGGCCGCAGACACGACAGGTCGCAACGGCAGCCGTGGCGCGACAGCTGG
GAGGGAGCAAGATGAAAGGTTCAGTAACTGCACTGCCCACTGCAAATAAC
TGCTGATAGGTTCATCTAAATTTCCCCAGCAGCAGCATATTCGGCGGTCC
TACAACTCTAAAAGAGCGATCTTTGGACGTTGAGTTGCGAATTCGCTGCT
CCAATATTTGGAACGACCTTGGCGCGGTCGTGGAGCTGACAGCGTAGCAA
TGGCATCCTCTCCGAGCATCTATCATCAAGGCAAGCACAGCCTGTTCACT
CAGCGGTTTCGCAAGATCGACGCAATTCGCAATGCTCTCCGAACGGCAGT
CACCGAAGAGCTCGCTCAGCAGACCCAGGAGAAGCGACGCGAGACCTACC
GGGTCAACGCGGAGAACAAGAGGAGATCGGCGGCCGCAGCTGCAGCGTTG
GACACCAAAACGGACACCAGCGGCAGTCTTCCTGGTAGCAATAGCGTCGG
TGATGGCCACGGTCCTGAAGTCTCAATCGCCGTGCCGTGCAGGCTGGGGT
GCGACGGAGCAGCCCGCAAGAAGACCCGTTCCGCCTCGTCCTCCTCCTTT
GGCAACAGTGGCGGCCGGGCTGTCTCACGGCCCCACGACAGACAGGGAGA
GCCTCTTAGCACCGCCGCGTGTGTTAAAATAGCATCCGCCGACCCGGACA
AGGCTTTTGGTTTGAGAGTTGATAGCGGCGACGCTCCGAGGGGAGAGGAA
GAGGGTGCCTCGGGCGCTGCTAGCACTTCCGGGGGTCGTCGTGATGACGA
TGATGACCACGGACCGACGCCTCCCACAGCAGCAGCAGCAGCAGCAGCAG
CAGCGGCAGCATCAGTGTTTACGGAAAGCCAATCAGCCTTCGCTTCCGCG
GGTGCTAAAGGAGGAGGCGGTGCCAAAGCGGGCGTTCGGTTTTTCGAAGA
CATCTTGCGTTTGGGCCCGAAAGGAACGCGTCCCAACGCTGTCGGAGGGG
CGCTGCCGAACTCGAAACGAAAACCTCACCCGCGCGTGCGTGGGGGTTGC
AAGGGCGAGGCGAGCAGAACCCAGGCCAACAAGAAGCCGCGGCAAGGGAC
AGGCGCGGTACCCGGACCGTTCGCTGGGCTGTCCCTTTGCGTGGTGCACC
TGGGTTCGGTGACCAGCAACACGGTCAAGGCATTCAAGCGGGGTGTCTGC
GACGGCGGAGGGCAAGTGGCGACGCGCTTCATGCCGGCCGATACGACCCA
CATCGTGGCGGACCCCTCGCTAGCCGCCTCCTGGGAGAAGCTGGACAACT
ACTTCAGCGGGCGGGGTGGCATCGATGACCTTGACGGTACCGTAGGCGGC
GGGGGGGTGGGGTTAAAGAGGGGC
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig1356:10978..12351+

>mRNA_E-fasciculatus_F_contig1356.2648.1 ID=mRNA_E-fasciculatus_F_contig1356.2648.1|Name=mRNA_E-fasciculatus_F_contig1356.2648.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=2250bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1356:10978..12351+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
ATGGCATCCTCTCCGAGCATCTATCATCAAGGCAAGCACAGCCTGTTCAC
TCAGCGGTTTCGCAAGATCGACGCAATTCGCAATGCTCTCCGAACGGCAG
TCACCGAAGAGCTCGCTCAGCAGACCCAGGAGAAGCGACGCGAGACCTAC
CGGGTCAACGCGGAGAACAAGAGGAGATCGGCGGCCGCAGCTGCAGCGTT
GGACACCAAAACGGACACCAGCGGCAGTCTTCCTGGTAGCAATAGCGTCG
GTGATGGCCACGGTCCTGAAGTCTCAATCGCCGTGCCGTGCAGGCTGGGG
TGCGACGGAGCAGCCCGCAAGAAGACCCGTTCCGCCTCGTCCTCCTCCTT
TGGCAACAGTGGCGGCCGGGCTGTCTCACGGCCCCACGACAGACAGGGAG
AGCCTCTTAGCACCGCCGCGTGTGTTAAAATAGCATCCGCCGACCCGGAC
AAGGCTTTTGGTTTGAGAGTTGATAGCGGCGACGCTCCGAGGGGAGAGGA
AGAGGGTGCCTCGGGCGCTGCTAGCACTTCCGGGGGTCGTCGTGATGACG
ATGATGACCACGGACCGACGCCTCCCACAGCAGCAGCAGCAGCAGCAGCA
GCAGCGGCAGCATCAGTGTTTACGGAAAGCCAATCAGCCTTCGCTTCCGC
GGGTGCTAAAGGAGGAGGCGGTGCCAAAGCGGGCGTTCGGTTTTTCGAAG
ACATCTTGCGTTTGGGCCCGAAAGGAACGCGTCCCAACGCTGTCGGAGGG
GCGCTGCCGAACTCGAAACGAAAACCTCACCCGCGCGTGCGTGGGGGTTG
CAAGGGCGAGGCGAGCAGAACCCAGGCCAACAAGAAGCCGCGGCAAGGGA
CAGGCGCGGTACCCGGACCGTTCGCTGGGCTGTCCCTTTGCGTGGTGCAC
CTGGGTTCGGTGACCAGCAACACGGTCAAGGCATTCAAGCGGGGTGTCTG
CGACGGCGGAGGGCAAGTGGCGACGCGCTTCATGCCGGCCGATACGACCC
ACATCGTGGCGGACCCCTCGCTAGCCGCCTCCTGGGAGAAGCTGGACAAC
TACTTCAGCGGGCGGGGTGGCATCGATGACCTTGACGGTACCGTAGGCGG
CGGGGGGGTGGGGTTAAAGAGGGGCATGGCATCCTCTCCGAGCATCTATC
ATCAAGGCAAGCACAGCCTGTTCACTCAGCGGTTTCGCAAGATCGACGCA
ATTCGCAATGCTCTCCGAACGGCAGTCACCGAAGAGCTCGCTCAGCAGAC
CCAGGAGAAGCGACGCGAGACCTACCGGGTCAACGCGGAGAACAAGAGGA
GATCGGCGGCCGCAGCTGCAGCGTTGGACACCAAAACGGACACCAGCGGC
AGTCTTCCTGGTAGCAATAGCGTCGGTGATGGCCACGGTCCTGAAGTCTC
AATCGCCGTGCCGTGCAGGCTGGGGTGCGACGGAGCAGCCCGCAAGAAGA
CCCGTTCCGCCTCGTCCTCCTCCTTTGGCAACAGTGGCGGCCGGGCTGTC
TCACGGCCCCACGACAGACAGGGAGAGCCTCTTAGCACCGCCGCGTGTGT
TAAAATAGCATCCGCCGACCCGGACAAGGCTTTTGGTTTGAGAGTTGATA
GCGGCGACGCTCCGAGGGGAGAGGAAGAGGGTGCCTCGGGCGCTGCTAGC
ACTTCCGGGGGTCGTCGTGATGACGATGATGACCACGGACCGACGCCTCC
CACAGCAGCAGCAGCAGCAGCAGCAGCAGCGGCAGCATCAGTGTTTACGG
AAAGCCAATCAGCCTTCGCTTCCGCGGGTGCTAAAGGAGGAGGCGGTGCC
AAAGCGGGCGTTCGGTTTTTCGAAGACATCTTGCGTTTGGGCCCGAAAGG
AACGCGTCCCAACGCTGTCGGAGGGGCGCTGCCGAACTCGAAACGAAAAC
CTCACCCGCGCGTGCGTGGGGGTTGCAAGGGCGAGGCGAGCAGAACCCAG
GCCAACAAGAAGCCGCGGCAAGGGACAGGCGCGGTACCCGGACCGTTCGC
TGGGCTGTCCCTTTGCGTGGTGCACCTGGGTTCGGTGACCAGCAACACGG
TCAAGGCATTCAAGCGGGGTGTCTGCGACGGCGGAGGGCAAGTGGCGACG
CGCTTCATGCCGGCCGATACGACCCACATCGTGGCGGACCCCTCGCTAGC
CGCCTCCTGGGAGAAGCTGGACAACTACTTCAGCGGGCGGGGTGGCATCG
ATGACCTTGACGGTACCGTAGGCGGCGGGGGGGTGGGGTTAAAGAGGGGC
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