mRNA_E-fasciculatus_F_contig1274.2084.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

BLAST of mRNA_E-fasciculatus_F_contig1274.2084.1 vs. uniprot
Match: D8LR74_ECTSI (Vacuolar protein 8 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LR74_ECTSI)

HSP 1 Score: 2425 bits (6284), Expect = 0.000e+0
Identity = 1374/1549 (88.70%), Postives = 1407/1549 (90.83%), Query Frame = 1

Query:    1 SQVFNNLATERTLRQRMIEEGAAPAIIALAASSHNRYLRSQCVEALCKLAVVPGSEAQIIAEGGVSCILAVQRAVPSLGELAGTVEEDESLERLLIQLLCNLSGFKNNQLKLVEEGAIRIISRAAERTASVDVVRLCASTLCNFAGEGRARPKMSDSRTAQALLNLTKHEDVGIRREVAHTIARLAADASCREKILQYGIIPILVTMSTAPDLDTTTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRLSCAQSLCNLIRFAIRLDYLIECGVVSALVRLADPGDEATSECCAAALYLFFCHPAVLALIDGREVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLLGLLQTESDKDLQADCAAALYNLARNQENCQAMIVSGAVPPIIVLAKSGSFETKTQCMSILQRMLLGRAMPEEIMTKSFVRTLLDMSKMEHRDTQQRVVIAVYRISCCERGRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAHRGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWKEIAMSNCMQAVYGLARSADEAVMSAGMKALHNVLLRAQHLEGVGHVFSGTVPIARRALMHESKEIRLVGVRLTNLVSTASVARQTAMLYRIVERVNVGDFDSDESLSYSFCCALERLALDPTTRSAVLHSGCINNFTALCAMSGRIMSRLAQFLYSVSCSPELVPSLVLEHDCIKVIAAVLEPV-GHEQDGAVEGRQGSGAGSGGGEGRKESLDGAKETCAALLFNLSTQDEVKTELVLKGGIRLAFALWEGASPEARRSCSLVACNLAVGKVNTAKMVRHGAGQLLVELVKNTDPLNDAGGNHVQLAEEGVIPALIDLAKTECPVVRRNCASAMRSMTCKAEVRQLLVASGAIKVILDDATNQDADESLAIDNELLCEIEAESWVNGTRGILVESRAPVLPMLEQNCSLLPAPADSRSQKAARVAAAAATEKEAGDALYSMPWQKLQQALDVDLREPEMEVSTKQDDAKRYDDPGINAALAGDTDADGPRGLPSSTTASCGKLEPKEASQAGRAMLGHPDDSAEEDSSLRGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX----------RVNPLMSLGSTPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRQQPSSSVSEATSXXXXXXXXLSNEQASWRRGKRRGGVQMLGRQHQLPGGIDGMPDPSIVDWEVAVVRASTNAEDMVEKYIQTRKEIHPGRRNSVNISSIGSPRRSSVLQPTSGGISSNVMALTASRRSSLLPGAASRRGSVQATGNRRASGVLFRPQYQATLDGTGNPAGNAAGQQGRRVSVSQPTFGASGAGRRVSRAGGSITTTNTTSTSKSATSLASVGEG-SYSELAIEALPHSEDTTSLTMAGTTQSSGNEGMLPLSAKEDASNDAAVDSGGAAGPLETVIVEASRGGIRSFMPIREGTFDAEAGRFPL 4611
            ++VFNNLATER+LRQRMIEEGAAPAIIALAAS                                     AVQRAVPSLG LAGTVEEDESLERLLIQLLCNLSGFKNNQLKLVEEGAIRIISRAAERTASVDVVRLCASTLCNFAGEGRARPKMSDSRTAQALLNLTKHED+GIRREVAHTIARLAADASCREKILQYGIIPILVTMSTAPDLDTTTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRLSCAQSLCNLIRFAIRLDYLIECGVVSALVRLADPGDEATSECCAAALYLFFCHPAVLALIDGREVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLLGLLQTESDK LQADCAAALYNLARNQENCQAMIVSGAVPP+IVLAKSGSFETKTQCMSILQRMLLGR MPEEIMTKSFVRTLLDMSKMEHRDTQQRVVIAVYRISCCERGRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAHRGMFKDGVVWGLAAMSRS DVTIAKLCSVALCNLSCEYWKEIAMSNCMQAVYGLARS+DEAVMSAGMKALHNVLLRAQHLEGVGH+FSGTVPIARRALMH+SKEIRLVGVRLTNLVSTA VARQTAMLYRIVERVNVGDFDSDE LSYSFCCALER ALDP TRS+VLHSGCI+NFTALCAMSGRIMSRLAQF+YSVSCSPELVPSLVLEHD +KVIAAVLEPV   E+DG+VEGRQG GAGS GGE RKESLDGAKETCAALLFN+STQDEVKTELVLKGGIRLAFALWEGASPEARRSCSLVACNLAVGKVNTAKMVRHGAGQLLVELVKNTDPLNDAGGNHVQLAEEGVIPALIDLAKT+CPVVRRNCASAMRSMTCKAEVRQLLVASGAIKVILDDATNQDADESL IDNELLCEIEAESWVNGTRGILVESRAPVLPMLEQNCSLLPAPADSR+QKAARVAAAAA EKEAGDALYSMPWQKLQ ALDVDLREP+MEVSTKQDDAKRYDDPGINAALAGDTDADGPRGLPSST+ASCGK+EPKEASQA R MLGHPDD+ EEDSSLRG XXXXXXXXXXXXXXXXXXXXXX                       +VNPLMSLGSTP   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX              XXXX    LSNEQASWRRGKRRGGVQM GRQHQ P GIDGMPDPS+VDWEVAVVRAS NA++MVE+YIQTRKEIHP RR SVNISSIGSPRRSSVLQPT GGI+SN MALTASRRSSL P AASRRGSVQA GNRRASGVLFRPQ+QAT  GTGNP GNAAG+Q RRVSVS PT  A GAGRR SRAGGSITTTNT STSKS TSLASVGEG + SEL I  L HS+DTTSLTMA TTQSSG EGMLPLS+ E   N    DSGGAAGPLETVIVEA+RGGIRSFMPIR GTFDAEAGRFPL
Sbjct:  202 AEVFNNLATERSLRQRMIEEGAAPAIIALAASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAVQRAVPSLGGLAGTVEEDESLERLLIQLLCNLSGFKNNQLKLVEEGAIRIISRAAERTASVDVVRLCASTLCNFAGEGRARPKMSDSRTAQALLNLTKHEDIGIRREVAHTIARLAADASCREKILQYGIIPILVTMSTAPDLDTTTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRLSCAQSLCNLIRFAIRLDYLIECGVVSALVRLADPGDEATSECCAAALYLFFCHPAVLALIDGREVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLLGLLQTESDKALQADCAAALYNLARNQENCQAMIVSGAVPPVIVLAKSGSFETKTQCMSILQRMLLGRTMPEEIMTKSFVRTLLDMSKMEHRDTQQRVVIAVYRISCCERGRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAHRGMFKDGVVWGLAAMSRSNDVTIAKLCSVALCNLSCEYWKEIAMSNCMQAVYGLARSSDEAVMSAGMKALHNVLLRAQHLEGVGHIFSGTVPIARRALMHKSKEIRLVGVRLTNLVSTALVARQTAMLYRIVERVNVGDFDSDEPLSYSFCCALERFALDPATRSSVLHSGCIDNFTALCAMSGRIMSRLAQFMYSVSCSPELVPSLVLEHDGMKVIAAVLEPVVQEEEDGSVEGRQGGGAGSXGGEDRKESLDGAKETCAALLFNMSTQDEVKTELVLKGGIRLAFALWEGASPEARRSCSLVACNLAVGKVNTAKMVRHGAGQLLVELVKNTDPLNDAGGNHVQLAEEGVIPALIDLAKTDCPVVRRNCASAMRSMTCKAEVRQLLVASGAIKVILDDATNQDADESLTIDNELLCEIEAESWVNGTRGILVESRAPVLPMLEQNCSLLPAPADSRTQKAARVAAAAAIEKEAGDALYSMPWQKLQ-ALDVDLREPDMEVSTKQDDAKRYDDPGINAALAGDTDADGPRGLPSSTSASCGKVEPKEASQASRTMLGHPDDTDEEDSSLRGXXXXXXXXXXXXXXXXXXXXXXXSSNTAFDNEEGHTDSTSHSSCGGKVNPLMSLGSTPEEDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSDGGLSNEQASWRRGKRRGGVQMFGRQHQPPAGIDGMPDPSVVDWEVAVVRASANADEMVERYIQTRKEIHPARRGSVNISSIGSPRRSSVLQPTGGGINSNAMALTASRRSSLFPAAASRRGSVQAAGNRRASGVLFRPQHQATQGGTGNPVGNAAGRQARRVSVSHPTIAAGGAGRRASRAGGSITTTNTXSTSKSVTSLASVGEGGTCSELEIGVLSHSDDTTSLTMADTTQSSGKEGMLPLSSTEHTGN----DSGGAAGPLETVIVEAARGGIRSFMPIRGGTFDAEAGRFPL 1745          

BLAST of mRNA_E-fasciculatus_F_contig1274.2084.1 vs. uniprot
Match: A0A6H5KQD4_9PHAE (Vacuolar protein 8 n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KQD4_9PHAE)

HSP 1 Score: 2007 bits (5200), Expect = 0.000e+0
Identity = 1245/1994 (62.44%), Postives = 1280/1994 (64.19%), Query Frame = 1

Query:  337 VEEGAIRIISRAAERTASVDVVRLCASTLCNFAGEGRARPKMSDSRTAQALLNLTKHEDVGIRREVAHTIARLAADASCREKILQYGIIPILVTMSTAPDLDTTTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRL----------------SCAQSLCNLIRFAIRLDYLIECGVVSALVRLADPGDEATSECCAAALYLFFCHPAVLALIDGREVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLLGLLQTESDKDLQADCAAALYNLARNQENCQAMIVSGAVPPIIVLAKSGSFETKTQCMSILQRMLLGRAMPEE----------IMTKSFVRTLLDMSKMEHRDTQQRVVIAVYRISCCERGRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAHRGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWKEIAMSNCMQAVYGLARSADEAVMSAGMKALHNVLLRAQHLEGVGHVFSGTVPIARRALMHESKEIRLVGVRLTNLVSTASVARQTAMLYRIVERVNVGDFDSDESLSYSFCCALERLALDPTTRSAVLHSGCINNFTALCAMSGRIMSRLAQFLYSVSCSPELVPSLVLEHDCIKVIAAVLEPVGHEQD--GAVEGRQGSGAGSGGGEGRKESLDGAKETCAALLFNLSTQDEVKTELVLKGGIRLAFALWEGASPEARRSCSLVACNLAVGK--------------------------VNTAKMVRHGAGQLLVELVKNTDPLNDAG----------------GNHVQLAEEGVIPALIDLAKTECPVVRRNCASAMRSMTCKAEVRQLLVASGAIKVILDDATNQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DADESLAIDNELLCEIEAESWVNGTRGILVESRAPVLPMLEQNCSLLPAPADSRSQKAARVAAAAATEKEAGDALYSMPWQKLQQALDVDLREPEMEVSTKQDDAKRYDDPGINAALAGDTDADGPRGLPSSTTASCGKLEPK----------------------------------------EASQAGRAMLGHPDDSAEEDSSLRGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--------RVNPLMSLGSTPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRQQPSSSVSEATSXXXXXXXXLSNEQASWRRGKRRGGVQMLGRQHQLPGGIDGMPDPSIVDWEVAVVRASTNAEDM----------------------------------------------------------------VEKYIQTRKEIHPGRRNSVNISSIGSPRRSSVLQPTSGGISSNVMALTASRRSSLLPGAASRRGSVQATGNRRASGVLFRPQYQATLDGTGNPAGNAAGQQGRRVSVSQPTFGASGAGRRVSRAGGSITTTNTTSTSKSATSLASVGEG-SYSELAIEALPHSEDTTSLTMAGTTQSSGNEGMLPLSAKEDASNDAAVDSGGAAGPLETVIVEASRGGIRSFMPIREGTFDAEAGRFPL 4611
            VEEGAIRIISRAAERTASVDVVRLCA TLCNFAGEGRARPKMSDSRTAQALLNLTKHED+GIRREVAHTIARLAADASCREKILQYGIIPILVTMSTAPDLD TTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRL                 CAQSLCNLIRFAIRLDYLIECGVVSALVRLADPGDEATSECCAAALYLFFCHPAVLALIDGREVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLL    TESDK LQADCAAALYNLARNQENCQAMIVSGAVPPIIVLAKSGSFETKTQCMSILQRMLLGRAMPEE          IMTKSFVRTLLDMSKMEHRDTQQRVVIAVYRISCCERGRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAHRGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWKEI MSNCMQAVYGLARS+D AVMSAGMKALHNVLLRAQHLEGVGH+FSGTVPIARRALMHESKEIRLVGVRLTNLVSTASVARQTAMLYRIVERVNVGDFDSDE LSYSFCCALER ALDP TRSAVLHSGC+NNFTALCAMSGRIMSRLA F+Y VSCSPELV SLVLEHD +KVIA VLE +G ++D  GAVEG QG GAGS  GEGRKESLDGAK TCAALLFN+STQDEVKTELVLKGGIRLAFALWEGASP+ RRSCSLVACNLAVGK                          VNTAKMVRHGAGQLLVELVKN DPLNDAG                GNHVQLA+EGVIPALIDLAKT+CPVVRRNCASAMRSMTCKAEVRQLLVASGAIKVILDDATNQ                                                                                                                                                                                                                                                                                                                                                                                                  DADESL IDNELLCEIEAESWVNGTRGILVESRAPVLPMLEQNCSLLPAPADSR+QKA RVAAAAA EKEAGDALYSMPWQKLQ ALDVDLREP+MEVSTKQDDAKRYDDPGINAALAGDTDADG RGLPSST+A+CGKLEPK                                        EA QA R MLGHPDD A+EDS  RGS            XXXXXX                         + NPLMSLGSTP       XXXXXXXXXXXXXX             XX        VSEATS        LSN+QASWRRGKRRGGVQM GRQ Q P GI GMPDPS+VDWEVAVVRAS NA++M                                                                VEKYIQTR+EIHP RR SVNISSIGS RRSSVLQPT GGI++NVMALTA+RRSSL P AASR GSVQA  NRRASGVLFRP++QA LDGTGNP GNAAG+QGRRVSVSQPT  A GA RR SRAGGSITTTN  STSKS TSLASVGEG + SEL    LPHSEDTTSLTMA TTQSSG  GMLPL + ED S +AAVDSGG AGPLETVIV+A+R GIRSFMP R GTFDAEAGRFPL
Sbjct:   22 VEEGAIRIISRAAERTASVDVVRLCARTLCNFAGEGRARPKMSDSRTAQALLNLTKHEDIGIRREVAHTIARLAADASCREKILQYGIIPILVTMSTAPDLDATTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRLRCGIDMRGIDGNGSQPGCAQSLCNLIRFAIRLDYLIECGVVSALVRLADPGDEATSECCAAALYLFFCHPAVLALIDGREVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLL----TESDKALQADCAAALYNLARNQENCQAMIVSGAVPPIIVLAKSGSFETKTQCMSILQRMLLGRAMPEERKHCNHYTHQIMTKSFVRTLLDMSKMEHRDTQQRVVIAVYRISCCERGRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAHRGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWKEITMSNCMQAVYGLARSSDAAVMSAGMKALHNVLLRAQHLEGVGHIFSGTVPIARRALMHESKEIRLVGVRLTNLVSTASVARQTAMLYRIVERVNVGDFDSDEPLSYSFCCALERFALDPATRSAVLHSGCVNNFTALCAMSGRIMSRLAYFMYCVSCSPELVASLVLEHDGMKVIAVVLE-LGSQEDEGGAVEGGQGGGAGSRVGEGRKESLDGAKATCAALLFNMSTQDEVKTELVLKGGIRLAFALWEGASPQVRRSCSLVACNLAVGKAAAPFSAAGTVDKVTKRNEPEKTFCQVNTAKMVRHGAGQLLVELVKNIDPLNDAGVRKRASAAFRNILCVSGNHVQLADEGVIPALIDLAKTDCPVVRRNCASAMRSMTCKAEVRQLLVASGAIKVILDDATNQTSGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXSAADPLATGGAIDELLAANDEDVERSKKRKYPEPGFAVDRSGAVGGRGSYSNAFKVKAAEFTRALCEDGKPLGNTGAAKVQGIAKKRIIAWVKEEGKLKGLIQAKPTLSDKFNSWYKRFRARRGYSTRRRTSVGQKLPNGHEGMKWGTLQKLRTALRGRAGQIFAERNPCASHELPLDGEELSSEQLESVMEEVFQELGNMDQTPVQREMPVEATLEIRGAKDARISTGAAEGAISAEVAPGNRTKFGHPVGGMSFGVQENSWCDAPEGSLWISESWRLCPNHESIKQRPSILVLDDFKCHRDKAFIDDLGKRCNTSVILIPGGLTPVLQLLDRGCRPHYGQAEPSGPWDGVYVDADESLTIDNELLCEIEAESWVNGTRGILVESRAPVLPMLEQNCSLLPAPADSRTQKAERVAAAAAIEKEAGDALYSMPWQKLQ-ALDVDLREPDMEVSTKQDDAKRYDDPGINAALAGDTDADGFRGLPSSTSANCGKLEPKVPLPPTRQTPYTWSCVSYGDSRTGQLSYSSPSSPHTKHFGEAYQASRTMLGHPDD-ADEDSFPRGSAGSSSSINGINCXXXXXXDDSSNFAFDNEEGHTNSTSHSSCGGKDNPLMSLGSTPEEHEDETXXXXXXXXXXXXXXDGRGSSWTRATKSXXXXXXXXXXVSEATSISSESDGGLSNDQASWRRGKRRGGVQMFGRQQQPPAGIGGMPDPSVVDWEVAVVRASANADEMSSRLGPATRRVCDWPEGDRSDHRSCSGHFFDIPARVPTRCDRSALAVCCSHLPVPMLTALLLQQVEKYIQTRREIHPARRGSVNISSIGSARRSSVLQPTGGGINNNVMALTAARRSSLFPAAASRHGSVQAASNRRASGVLFRPEHQAPLDGTGNPVGNAAGRQGRRVSVSQPTIAARGARRRASRAGGSITTTNXXSTSKSVTSLASVGEGGTCSELKKGVLPHSEDTTSLTMADTTQSSGKVGMLPLCSTEDTSTNAAVDSGGTAGPLETVIVKAARSGIRSFMPKRGGTFDAEAGRFPL 2008          

BLAST of mRNA_E-fasciculatus_F_contig1274.2084.1 vs. uniprot
Match: F0YJH6_AURAN (Vacuolar protein 8 n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YJH6_AURAN)

HSP 1 Score: 357 bits (917), Expect = 7.910e-98
Identity = 299/1001 (29.87%), Postives = 462/1001 (46.15%), Query Frame = 1

Query:  271 LERLLIQLLCNLSGFKNNQLKLVEEGAIRIISRAAERTASV-------------DVVRLCASTLCNFAGEGRARPKMSDSRTAQALLNLTKHEDVGIRREVAHTIARLAADASCREKILQYGIIPILVTMSTAPDLDTTTGRCIALAFRVLS----SDRKFAEI----IVDGGAVDALISLTRSTDSPCRLSCAQSLCNLIRFAIRLDYLIECGVVSALVRLADPGDEATSECCAAALYLFFCHPAVLALIDGREVIRALMDLCRVG-TTSVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLLGLLQTE---------------------SDKDLQADCAAALYNLARNQENCQAMIVSGAVPPIIVLAKSGSFETKTQCMSILQRMLLGRA-----MPEEIMTKSFVRTLLDMSKMEHRDTQQRVVIAVYRISCCERGRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAHRGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWKEIAMSNCMQAVYGLARSADEAVMSAGMKALHNVLLRAQHLEGVGHVFSGT-VPIARRALMHESKEIRLVGVRLTNLVSTASVARQTAMLYRIVERVNVGDF--DSDESLSYSFCCALERLALDPTTRSAVL--HSGCINNFTALCAMSGRIMSRLAQFLYSVSCSPELVPSLVLEHDCIKVIA----AVLEPVGHEQDGAVEGRQGSGAGSGGGEGRKESLDGAKETCAALLFNLSTQDEVKTELVLKGGIRLAFALWEGASPEARRSCSLVACNLAVGKVNTAKMVRHGAGQLLVELVKNTDP--------------LNDAGGNHVQLAEEGVIPALIDLAKTECPVVRRNCASAMRSMTCKAEVRQLLVASGAIKVILDDATNQDAD-ESLAIDNELLCEIEAESWVNGTRGILVESRA--PVLPMLEQNCSLL 3051
            +E++L+  LCNLS  KNNQ +LVEEGA+RI++R      +              +   LCA TL N +   R+R KM+  R    LL++ K  D  I++  A TI RLA D SC EKI+  G +  +VTMS     D  TGR  A A R L      +  F ++    I D  AV A+ISL    D+    +C++++ +L +  + +D +I  G + A+V L+   D      C+ ALY   C     A++      +AL DLC      ++ +R  A +WN+T   +       A   IP L+ LL  E                     S  + +A    AL  LA +  NC+ M+ +GA+ PI+ L++SG+  T+ QC +IL R     A     + E+ +++ F+  LLD+S +E   TQQR + AV  +S     R L+L  G    L+ L +KP+E++R+GCA T  N+A D G E  + +  AV  LLI ALV+SD  ET+  C  AL NLL  +  H+ M  DGV+WGLA +       IAKL    +          +A+ + M+ V  + +S      S   + L N L++   LE        T   I +      S +  L  +RL  L   +  A +      I+  ++      D   +L+Y+  C    LA+ P+T +A +  H+  +     +  ++G     LA+++Y V+   +   +L+ ++    + A    A L P                                   C   L+NLS +  V+  LV +G I      W       R   +L+ACNLA G+VNTA++V+ G G +++  + + D               L     N   +A  G I  L+ LA  + P+  ++CASA+R  T     R++LV SGAI VIL D+       + L + + LLC++E ESW NG+R    E R   P  P+L ++  L+
Sbjct:  315 MEQMLVGCLCNLSCLKNNQGRLVEEGAVRIVNRIFRAAVAGRPWVKPGRDQVLHETTLLCAKTLSNLSSCSRSRSKMTAQRVIIVLLDMAKLADEDIKQSCALTITRLAMDVSCCEKIIHQGAMTAIVTMSQCGSKDIITGRLCAAALRQLCITGFENHLFEKMPRAQIKD--AVKAVISLI-GPDTRTNQNCSRTIYSLFQTEL-VDTMINDGALDAIVTLSGSRDSTIVMWCSRALYHLSCFKTHAAVMLEHGCQQALCDLCDTHYPDAIVRRSAATLWNLT---RATSDNIDATALIPALIDLLAREEAAPELALVKSPSFSGAALSASGSNTKAYAIGALAQLAYSSANCRRMLAAGALMPIVELSRSGNLITRIQCGAILSRFSFQAAYRAHMLSEQRLSEQFLDALLDLSTLEDCPTQQRFIHAVVNMSYEPNARRLMLTNGVLRVLVDLSNKPDEQIRRGCAATFCNIAADEGSEELVLQANAVPALLIIALVSSDRPETKEICAAALCNLLHSESTHQSMVNDGVIWGLAKLG------IAKLHIHLI----------LALPSAMKVVRAMMQSKVATTRSVAARTLLNFLIKLPTLEASNIDHEATKFSIFKMTEQLGSTDEGLTRIRLLALCLLSQSAPELITTQGILPPLDARSACADPQSALAYAHLCC--NLAMSPSTDNATIASHAPALAQLLEIPNINGHYRLELARWVYCVTSRIDAAETLMTKNIMNIIFALASTATLIPF----------------------------------CVTALYNLSVEANVQASLVQQGAIEFTVDHWAMMGESERLLAALMACNLACGEVNTARIVKSG-GSMVISYLASPDSSPFQCRCCAIALRNLVRVAANQRPMAASGAIDTLVALAALDDPLTTQHCASALRIFTYNKTTREVLVKSGAISVILADSQGSTGTGDDLQLSHSLLCKVETESWKNGSRSGQQEGRTRLPAPPVLIESAHLI 1255          

BLAST of mRNA_E-fasciculatus_F_contig1274.2084.1 vs. uniprot
Match: A0A482SV17_9ARCH (Vacuolar protein 8 n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SV17_9ARCH)

HSP 1 Score: 343 bits (879), Expect = 4.350e-93
Identity = 284/1059 (26.82%), Postives = 501/1059 (47.31%), Query Frame = 1

Query:   94 SSHNRYLRSQCVEALCKLAVVPGSEAQIIAEGGVSCILAVQRAVPSLGEL---------------AGTVEEDESL-------------ERLLIQLLCNLSGFKNNQLKLVEEGAIRIISRAAERTASVDVVR-LCASTLCNFAGEGRARPKMSDSRTAQALLNLTKHEDVGIRREVAHTIARLAADASCREKILQYGIIPILVTMSTAPDLDTTTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRLSCAQSLCNLIRFAIRLDYLIECGVVSALVRLA-DPGDEATSECCAAALYLFFCHPAVLALIDGREVIRALMDLCRVGTT-SVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLLGLLQT-ESDKDLQADCAAALYNLARNQENCQAMIVSGAVPPIIVLAKSGSFETKTQCMSILQRMLLGRAMPEEIMTKSFVRTLLDMSKMEHRDTQQRVVIAVYRISCCERGRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAHRGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWKEIAMSNCMQA-VYGLARSADEAVMSAGMKALHNVLLRAQHLEGVGH--VFSGTVPIARRALMHESKEIRLVGVRLTNLVSTASVARQTAMLYRIVERVNVGD-FDSDESLSYSFCCALERLALDPTTRSAVLHSGCINNFTALC-AMSGRIMSRLAQFLYSVSCSPELVPSLVLEHDCIKVIAAVLEPVGHEQDGAVEGRQGSGAGSGGGEGRKESLDGAKETCAALLFNLSTQDEVKTELVLKGGIRLAFALWEGASPEARRS--CSLVACNLAVGKVNTAKMVRHGAGQLLVELVKNTDPLNDAG----------------------GNHVQLAEEGVIPALIDLAK---------------------TECPVVRRNCASAMRSMTCKAEVRQLLVASGAIKVILDDATNQDADESLAIDNELLCEIEAESWVNGTRGILVESRAPVLP 3024
            +S+ R ++  C +A+C L  V G E +++ EG    +L +  A P   ++               A   +  ESL             E+ +I  LCNLS  +NNQL+LVE+G +RI+ +  +  +  DV+R +    L NF  E RAR K+ D      ++ +++     IR   A  +  L+ D + R KI+      +++        +   G+  +   RVL SD+   E +++ G   A++SL  S D+     C +SLC L +    L+ L++ G    +VRLA    D AT+E C+ ALY           +  + ++  ++ LC   ++  V+  C AA+   T ++++       + +IP+L  LL + +   +++  CA +L+NLA + +NC  ++ +GA+ P++ L ++   +TK  C  I+ R+ L     ++  T   ++ LL++S ++ R TQ+RVVIA+  +S  +   E LL+ G    ++ L S+ +E +R+GC + + N++Y  G E  I + G V  L+IT+L+ SD   +R  C KAL NL+ ++  ++ M KDGV+WGL+ ++ ++D  + +L + ALC LSC + +EI  S+  +  V  L +S +  ++    + L N+LL     +      +     P+A    +   +E+  + V    L S +  +R   +   ++ +++    F +D  +SY++      +A +P  R+ VL    I  F  +       ++  + Q +Y +SCS E +P LV +H  I  I   +E                       EG   ++        A LFNL+T   + + LV +G + +   LW     + + +    L  C+LA G+ N++++V  G   LL  + ++      A                        N  Q+ +EGV+P LI +A                      +E  ++R NCA+A++S+T   E+R +LV + AI +IL +   +     L+I   LL E+EAESW NG R    + RA  LP
Sbjct:  174 NSNIREIKGDCAKAICNLCCVSGYEFKMVKEGVPFAMLNIAAACPETLDICLKTLLNISCIPDKFARIEDVTESLLYFVNHNSLTVSQEQTMIAALCNLSALRNNQLRLVEDGCLRIVEKYFK--SKYDVLRRMVCEMLKNFTSESRARAKLLDIDIISVIVVMSQDTLEEIRILSAKCLYFLSKDRNFRRKIVHSSAFGLILDACRMSKSNIEMGQVASKTLRVLCSDKDLPEKLIEDGVGAAILSLMDSEDNVIHQYCTESLCLLFQHKDVLNNLLDEGAPDMVVRLAYKTRDLATAEWCSFALYQLTESTVCDFHVMEQSILPCILYLCESSSSPQVKLFCSAALAYATLIKRI-----DCSAAIPLLSKLLDSADCSAEIKKYCATSLFNLADDAKNCSEILEAGALAPVVKLTQTS--DTKVICAGIISRLSLHEQYYDKFATGDVLKVLLELSSIDDRLTQRRVVIALSNLSHSDVLCEKLLQLGPISYIVSLASERDESLRRGCISIVCNMSYRPGTEKAIVQGGIVPTLMITSLITSDQVVSRVICVKALVNLMADRSLYKSMVKDGVIWGLSKLALTEDDELQELSAQALCRLSCYFAREIINSSVAKTTVMRLLKSNNLDIVRPAARILTNLLLATNESDEKFRTDIVENMHPMAS---IQSDEELNALCVVCLCLASQSEGSRIAIVDSGMLNKIDPSAIFSADHRISYAYITMFNNIANNPVMRTKVLDDHMIERFEKILETRDSNLLLAVVQSMYYISCSSENIPKLVDQH-IIPFIHFAIET----------------------EGELSTI--VLHHLMACLFNLTTSQNMLSTLVSQGMVEVIVQLWPDLVKDLKMAKLALLSLCHLACGRTNSSRVVSDGITPLLCFITEHRKQPQFAQYTFTYDYYLRISAALRNLLIVVSNQKQMIDEGVLPVLIQMANQSFDRLIAANNLTTTLGAATPSETKLIRINCAAALKSLTYNKELRSMLVETEAINIILAEIRKE---SDLSISQGLLKELEAESWENGARTKQKDGRAKTLP 1192          

BLAST of mRNA_E-fasciculatus_F_contig1274.2084.1 vs. uniprot
Match: A0A6G0XWU8_9STRA (Vacuolar protein 8 n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XWU8_9STRA)

HSP 1 Score: 102 bits (253), Expect = 7.210e-18
Identity = 218/1064 (20.49%), Postives = 400/1064 (37.59%), Query Frame = 1

Query:   49 MIEEGAAPAIIALAASSHNRYLRSQCVEALCKLAVVPGSEAQIIAEGGVSCILAVQRAVPS---------------LGELAGTVEED---------ESLERLLIQLLCNLSGFKNNQLKLVEEGAIRIISRAAERTASVDVVRLCASTLCNFAGEGRARPKMSDSRTAQALLNLTKHEDV--GIRREVAHTIARLAADASCR-------------EKILQYGIIPILVTMSTAPDLDTTTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTR--STDSPCRLSCAQSLCNLIRFAIRLDY----------------------LIECGVVSALVRLA-DPGDEATSECCAAALYLFFCH-PAVLALIDGREVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLLGLLQTESDKDLQADCAAALYNLARNQENCQAMIVSGAVPPIIVLAKSGSFETKTQCM-SILQRMLLGRAMPEEI-MTKSFVRTLLDM--------------------SKMEHRDTQQRVVIAVYRISCCER---------------------GRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAH--RGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWKEIAMSNCMQAVYGLARSADEAVMSAGMKALHNVLLRAQHL-----EGVGHVFSGTVPIARRALMHESKEIRLVGVRLTNLVSTASVARQTAMLYRIVERVNVGDFDSDE--SLSYSFCCALERLALDPTTRSAVLHSGCINNFTALCAMSGRIMSRLAQFLYSVSCSPELVPSLVLEHDCIKVIAAVLEPVGHEQDGAVEGRQGSGAGSGGGEGRKESLDGAKETCAAL-LFNLS-TQDE-VKTELVLKGGIRLAFALWEGASPEARRSCSLVACNLAVGKVNTAKMVRHGAGQLLVELVKNTDP------------LNDAGGNHVQLAEEGVIPALIDLAKTEC---PVVRRNCASAMRSMT 2835
            +++ G  PA+++LAA   ++  +  C  A   LA VP +   ++  G VS I  + R   S               L  + G + E+         E ++R+L++L     G      ++V      +   AA+    +D    C + +C         P  +     +AL NLT+ +     +  E AH +        C+             E  +++G+  + +  ST        GR   +   ++SS    A + + G   D + SL    S D  CR   A  +  ++   I                          L+ CGVV  L+++A D   E      +    LF C  P   + +D   +I  L+ L +    S+    + A++N++  + +       A +I   L  L T+ DK +       +Y L+    +   M+  G   PI     S +   +   + ++LQ      A P+     KS V++L  +                    +  ++RD   R  + VY    C                         E+++ E A   LI L    N+ +++ C  +   ++     E ++ + GA++  +I ALVA+ SD+ +  C K ++N LC +  H  R M   GV W L+++S          C+   CNLS  Y  ++  +   +A+  L  + D   +   ++A+ N+L   +       EG+  V    V  +   + H +  + L   R  +     ++  Q  +L  I+  ++     +    +L  S   A+ +  + P    ++L +       AL A          Q LY+++C P  +  L+  H    V       V H  DG                    + D    T   + L+NL+  +DE   + LV  G   +   ++   +   +  C L  CNLA+GKVN++++V    G +L++ V+   P            L +  GN   + + GV+ + + L +      P++R  C  A+  +T
Sbjct:  151 LLDNGIVPALVSLAAVPDSQT-QLHCARAFYGLAQVPCTRRLLVLNGVVSTIALLVRHGESSQSTSSSGRTRLKQDLAAILGHISEESFLEDKMLREGVDRILVKLY---QGHNVETKRIVALTFFNLSHNAAQIKHFIDSFTQCITAICKTVAVP-THPNKATLLLVKALFNLTQVQSFHNSLMAESAHRLLSTPLAHFCKCHKTKTKPVEPVEEDAIEFGLKGLFMLASTK------NGRLHIVQDNLISSI--VACLALGGTIADVVCSLLYQLSLDETCREPLAHEMQGVVDGIIHCSTYGYFTISWVLRHLCATRAIFPTLVACGVVPLLMQMARDTHSEIKLNGISCMCCLFQCDLPDAASCVD--RIIGDLVQLLQSNVESITVFAITALFNLSCNDTLLPL--LCATNIVDALKPLFTKPDK-VSTALLPLIYQLSSVTRS--RMLEVGYFDPIAAAISSTTGSARHAALDTMLQFTAEPTAFPQGTDEVKSLVKSLYTLKTNADNTALRSCVSILAHLTTTPKNRDMLLRTGVTVYLARVCNAVDDFVMTNCAYILFCLTETLDAVEIIVREEAIPVLIHLSRASNDYVKELCILSFCRISSHPTMEAKLVEQGAIASSMIMALVATKSDQIKTLCVKIISNCLCVQSKHCTRTMVDHGVFWALSSLSTLTFSDTKHACATCFCNLSTSYPLKMVEAGVPRALVQLVETGDGPTIVVALEAIANLLSNEKACGILVNEGLVKVLQCLVDDSNANVAHAAARVMLQISRSDDRCRVDNL--QNGLLQWILSVLSDDTLATQALITLCDSTAIAIAKEHIRPLPALSILRA-------ALHATDQARRGLCMQTLYNMTCQPTSLLELI--HAEAHVF------VQHLFDG--------------------NDDPVLATLGVISLYNLTCVEDEGALSNLVTSGLTAVLHHIYLFDTATDKTWCVLAICNLALGKVNSSRIVSENGGVVLIDFVQKASPEVAATIAAAFRKLINPPGNQKMMVDLGVVASFVYLLRHHAANYPLIRLRCLDALAILT 1157          

BLAST of mRNA_E-fasciculatus_F_contig1274.2084.1 vs. uniprot
Match: A0A067CXH3_SAPPC (Vacuolar protein 8 n=1 Tax=Saprolegnia parasitica (strain CBS 223.65) TaxID=695850 RepID=A0A067CXH3_SAPPC)

HSP 1 Score: 100 bits (248), Expect = 2.810e-17
Identity = 154/787 (19.57%), Postives = 284/787 (36.09%), Query Frame = 1

Query:  532 VAHTIARLAADASCREKILQYGIIPILVTMSTAPDLDTTTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRLSCAQSLCNLIRFAIRLDYLIEC---------------------------------------------GVVSALVRLA------DPGDEATSECCAAALYLFFCHPAVLALIDGREVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLLGLLQTESDKDLQADCAAALYNLARNQENCQAMIVSGAVPPIIVLAKSGSFETKTQCMSILQRMLLGRAMPEEIMTKSFVRTLLDMSKMEHRDTQQRVVIAVYRISCCERGRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIE--ITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAH--RGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWKEIAMSNCMQAVYGLARSADEAVMSAGMKALHNVLLRAQHLEGVGHVFSGTVPIARRALMHESKEI-RLVGVRLTNLVSTASVARQTAMLYRIVERVNVGDFDSDESLSYSFCCALERLALDPT------TRSAVLHSGCINNFTALCAMSGRIMSRLAQFLYSVSCSPELVPSLVLEHDCIKVIAAVLEPVGHEQDGAVEGRQGSGAGSGGGEGRKESLDGAKETCAALLFNLST--QDEVKTELVLKGGIRLAFALW--EGASPEARRSCSLVACNLAVGKVNTAKMVRHGAGQLLVELVK 2694
            V   + +L+ D +CR+ I+      +   +   P+        ++  FR L + +     +VD G +  L+ ++R      +L+    +   + F + L   ++C                                             G+V AL +L         G++A +      LY   C+     L+   +    +       +  VR+  +  + N +  E     G    +S+   L  L+T  D      C + L +L     N + ++ +G V  +  +  S        C  IL                         S  E  D   RV                 L EGA   LI+L    ++ ++  C  T   ++   G  +E  +   GA++  +I ALVAS SD  + +C + L+N L  +  H  + M ++GV W L++++   D    ++C++  CN++  Y  ++  +   +++  L  + D   +   ++A+ N++   +    +  V  G V + R  + ++  ++     + L  +       R        V+ V  G     +S+ +    AL+ L +  T       R  ++    ++   A+ A      S   Q +Y+ SC   L   LV  H     +A+ L     E  G                            CA  + NL+    D+V   LV  G   L   L+  E  +   R  C L  CNLA+GKVNTA++V    G +L++ V+
Sbjct:  409 VCSILYQLSTDEACRDAIVHE----VKAIVEAMPNCSPYGYFALSWVFRNLCATKTIYPSLVDAGVLPVLLDMSRHDHDEVKLNGISCVVCFLEFDLHLQAAVDCLEFILQDLIGLLSAPQPTIVIFATTALFNLSCNDALQPLLCAPKAGLVPALQQLVVVRRAHTQGEKAVTAALLPLLYRLSCNADNRFLMVQADFFGFVAAAIPSTSAHVRQAALDTMLNFSTEEDHFPQGTDDVKSLLKTLYALKTNHDAPALRSCVSLLSHLTATPPNRELLLKTGCVVCLERMCNSADDFIMANCAHILY------------------------SLTETADAVDRV-----------------LREGAIPILIQLSRASSDHVKHLCLMTFTRISSFSGINVETKLVDQGAIAAAMIMALVASKSDAIKTSCVQLLSNCLSIQSKHCTKVMVENGVFWALSSLATLPDAHTKRICAICFCNIAIAYPVKMVEAGVPRSLVHLLEAGDTDTLVVALQAIANIVQNDKACAIL--VSEGLVKLLRSLVENKHPQVWHAAAIALLQMTRADDKCR--------VDNVRNGIIPWMQSVLHEQSIALQCLVMLGTISAYEPARKLMVAPDLMDLLRAVSAPDLARQSLCVQIVYNTSCELSLAADLVRGH-AHAFLASCLPGGSPELQGM---------------------------CAGAVHNLTCVQNDDVVATLVQSGVGMLLQTLYMLETLAAADRVWCVLAICNLALGKVNTARLVHDNGGVVLIDFVQ 1112          

BLAST of mRNA_E-fasciculatus_F_contig1274.2084.1 vs. uniprot
Match: A0A485KZE1_9STRA (Vacuolar protein 8 n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KZE1_9STRA)

HSP 1 Score: 95.1 bits (235), Expect = 9.490e-16
Identity = 168/826 (20.34%), Postives = 319/826 (38.62%), Query Frame = 1

Query:  532 VAHTIARLAADASCREKILQYGIIPILVTMSTAPDLDTTTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRLSCAQSLCNLIRFAIRLDYLIEC--GVVSALVRLADPGDEATSECCAAALYLFFCHPAVLALI--DGREVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVAHSGGSA---AESIPMLLGLLQTESDKDLQADCAAALYNLARNQENCQAMIVSGAVPPIIVLAKSGSFETKTQ-CMSILQRMLLGRAMPEEIMTKSFVRTLLDMSKMEHRDTQQRVVIA--VYRISCCER---GRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAH--RGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWKEIAMSNCMQAVYGLARSADEAVMSAGMKALHNVLLRAQHL-----EGVGHVFSGTVPIARRALMHESKEIRLVGVRLTNLVSTASVARQTAMLYRIVERVNVGDFDSDESLSYSFCCALE--RLALDPTTRSAVLHSGCINNFTALCAMSGRIMSRLAQFLYSVSCSPELVPSLVLEHDCIKVIAAVLEPVGHEQDGAVEGRQGSGAGSGGGEGRKESLDGAKETCAALLFNLSTQDE-VKTELVLKGGIRLAFALWEGASPEARRS-CSLVACNLAVGKVNTAKMVRHGAGQLLVELVKNTDPLNDAG------------GNHVQLAEEGVIPALIDLAK--TECPVVRRNCASAMRSMTCKAEVRQLLVASGAIKVILD 2895
            V   + +L+ D +CR+ ++      I V + +           ++  FR L + +     +V+ G V  L+ ++R T    +L+    +C L++  ++L   + C   ++  LV+L     ++       AL+   C+ A+ + +      V+ AL  L       +  +  AA+  +  V Q++H    A   A     +   +   +     A     L+ +    E  Q      A+   +   K+ S     + C+S+L  +     MP        +RT   +      +T    V+    Y + C        E+++ E A   LI+L    ++ +++ C  +   ++     E ++ + GA++  +I ALVA+ SD  +  C K ++N LC +  H  R M   GV W L++++          C+   CNLS  Y  ++  +   +A+  L  + D   +   ++A+ N+L   +       EG+  +    V     ++ H +  + L   R  +     S+     +L  ++  +      +   ++   C A    +  ++PT   AVL +        L A   R +    Q LY+++C    +  LV  H  +      L+ V    D A+                      A              DE   + LV  G  R+   ++   +     S C    CNLA+GKVN++++V    G +LV+ V+    L+DA             GN   + E GV+ AL+ L +  T     R +C  ++  +T   E     +  G +   LD
Sbjct:  422 VCSILFQLSLDETCRDSMVHE----ISVVVDSIQQCSAYGYFAVSWVFRNLCATKAIYPALVEAGVVRLLMQMSRDTHDEIKLNGISCMCCLLQCDVQLADAVACMQRIMLDLVQLLQSPTDSIVVFAITALFNLSCNDALHSFLADTATGVVPALQTLL---AKPLNDKTTAALLPL--VFQLSHESRIAMLRAGFFDFVAAAITATTGAARHAALDTMLHFMLEPDEFPQGTDEVKALLKALYTLKTNSDPVALRSCVSLLAHLT---TMPRN--RDLLLRTGCALCLARICNTADDYVMGNCAYILFCLTETLDAVEIIVREDAIPVLIQLSRASSDLVKELCIRSFCRISTHANMETKLVEQGAIAASMIMALVATKSDTIKTLCVKIISNCLCLQAKHCTRTMVDHGVFWALSSLASLPFADTKHACATCFCNLSTAYPLKMVEAGVPRALVQLVETGDGPTIVVALEAIANLLQNDKACTILVNEGLVKILRRLVQDTNASVAHAAAMVMLKTSRADDKCRVESLRND--LLPWMLTILADAALATQALITLCDCSATPAAKTHVEPTAAMAVLRAA-----VDLDATDRRRLRFCVQTLYNLTCQRASLLDLVRAHAHV-----FLQDVLPTDDAAL----------------------AHLXXXXXXXXXXVDDESALSSLVTSGLTRVLHRVYLADAASTTASWCVHSICNLALGKVNSSRIVTELGGVVLVDYVQAATTLDDATVSAAFRKLINPPGNQKAMVELGVVRALVRLLRHPTAAASTRLHCLDSLAILTRNKENILRSLGDGILACTLD 1199          

BLAST of mRNA_E-fasciculatus_F_contig1274.2084.1 vs. uniprot
Match: A0A024T8J9_9STRA (Vacuolar protein 8 n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024T8J9_9STRA)

HSP 1 Score: 93.6 bits (231), Expect = 2.820e-15
Identity = 152/756 (20.11%), Postives = 289/756 (38.23%), Query Frame = 1

Query:  532 VAHTIARLAADASCREKILQYGIIPILVTMSTAPDLDTTTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRLSCAQSLCNLIRFAIRLDYLIECG--VVSALVRLADPGDEATSECCAAALYLFFCHPAVL--------ALIDG-REVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVA--HSG--GSAAESIPMLLGLLQTESDKDLQADCAAALYNLARNQENCQAMIVSGAVPPIIVLAKSGSFETKTQCMSILQRMLLGRAMPEEIMTKSFVRTLLDMSKMEHRDTQQRVVIAVYRISCCERGRELLLEEGAPESLIRLISKPNEEMRKGCANTLLNLAYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAH--RGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWKEIAMSNCMQAVYGLARSADEAVMSAGMKALHNVLLRAQHL-----EGVGHVFSGTVPIARRALMHESKEIRLVGVRLTNLVSTASVARQTAMLYRIVERVNVGDFDSDESLSY----SFC-CALERLA---LDPTTRSAVLHSGCINNFTALCAMSGRIMSRLAQFLYSVSCSPELVPSLVLEHDCIKVIAAVLEPVGHEQDGAVEGRQGSGAGSGGGEGRKESLDGAKETCAALLFNLS-TQDEVKTELVLKGGIRLAFALWEGASPEARRS-CSLVACNLAVGKVNTAKMVRHGAGQLLVELVKNTD 2703
            V   + +L+ D SCR+ ++      +   +     + T     ++  FR + + R     +V  G V  L+ +TR      +L+    +C L++  ++LD    C   ++  LV+      E+       AL+   C+  ++         L+D  + VIR      R  T  V    +  I+ +    +V    SG     A +I    G  +  +  D     A       +  ++ +A++ +     +  L  +        C+S+L  +       + ++       L  +               +Y ++      E+++ E A   LI+L    N+ +++ C  T   ++     E ++ + GA++  +I ALVA+ SD+ +  C K ++N L  +  H  R M   GV W L+++S          C+   CNLS  Y  ++  +   +A+  +  + D   +  G++A+ N+L   +       EG+  +    V  +  A+ H +  + L   R  +     S+        R +         S+ESL      + C C   R A   + P     +L    ++          R M    Q LY+++C P  +  LV                   Q GA    Q         +G +         C   L+NL+   DE     ++  G  L   L +    +  ++ C L  CNLA+GKVN++++V    G +LV+ V++ +
Sbjct:  410 VCSILYQLSMDESCRDAMVH----DMATIVDNIVHISTYGYFTMSWVFRHVCATRAIYPSLVVAGVVPVLMQMTRDAHDEIKLNGISCMCCLLQCDLQLDDATGCTERIIVDLVQFLQSPIESIVVFAITALFNLSCNDTLIPFLSAPSTGLVDALKHVIRPPSHSHRRPTEKVTNVMLPLIYQLAPTTRVVMLQSGFFDFVATAISTTSGAPR-HAALDTMVQFALDPAEFPQGTDDVRALVKA-----LYTLKTNSDTIALRSCVSLLAHLTTSPKNRDVLLKTGCAVCLARICNTADDFIMGNCAYILYCLADTIEAVEVIVREEAIPVLIQLSRASNDTVKELCILTFCRISAHSNMETKLVEQGAIAASMIMALVATKSDQIKTLCIKIISNCLSVQAKHCTRTMIDHGVFWALSSLSTLPFADTKHACATCFCNLSSAYPLKMIEAGVPRALVQIVETGDGPTIVVGLQAIANLLHNDKACGILVNEGIVKILRRLVDDSNAAVAHAAAMVFLKTSRADDKCRIESIRNGLLPWMRSIL--------SNESLVLQCLITLCDCTANRAAQVHMHPAHAMDILRIASMSTALTSATTPDR-MGLTVQALYNLTCQPSKLLELV-------------------QAGAHNFLQ---------QGLRADDRDLSSLCVLSLYNLTCVLDETALSALVASG--LTQVLHDTYLLDLEKAWCVLSICNLALGKVNSSRIVMENGGVVLVDYVQSNE 1116          

BLAST of mRNA_E-fasciculatus_F_contig1274.2084.1 vs. uniprot
Match: A0A425DEE5_9STRA (Vacuolar protein 8 n=12 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A425DEE5_9STRA)

HSP 1 Score: 90.9 bits (224), Expect = 1.850e-14
Identity = 205/996 (20.58%), Postives = 375/996 (37.65%), Query Frame = 1

Query:   49 MIEEGAAPAIIALAASSHNRYLRSQCVEALCKLAVVPGSEAQIIAEGGVSCI-LAVQRAVPS-----LGELAGTVEEDESLERLLI-----QLLCNLSGFKNNQLK-LVEEGAIRIISRAAERTASVDVVRLCASTLCNFAGEGRARPKMSDSRTAQALLNLTKHEDV--GIRREVAHTIARL----------------------------------AADASCREKILQYGIIPILVTMSTAPDLDTTTGRCIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRLSCAQSLCNLIRFAIRLDYLIECGVVSALVRLA-DPGDEATSECCAAALYLFFCHPAVL-ALIDGREVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLL-GLLQTESDKDLQADCAAALYNLARNQENCQAM-----IVSGAVPPIIVLAKSGSFETKTQ--------------CMSILQRMLLGRAMPEEIMTKSFVRTLLDMSKM-EHRDTQQRVVIAVYRISCCERGR---------------------ELLLEEGAPESLIRLISKPNEEMRKGCANTLLNL-AYDHGREIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAH--RGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWKEIAMSNCMQAVYGLARSADEAVMSAGMKALHNVLLRAQHLEGVGHVFSGTVPIARRALMHESKEIR----LVGVRLTNLVSTASVARQTAMLYRIVERVNVGDFDSDESLSYSFCCALERLA---LDPTTRSAVLHSGCINNFTALCAMSGRIMSRLAQFLYSVSCSPELVPSLVLEHDCIKVIAAVLEPVGHEQDGAVEGRQGSGAGSGGGEGRKESLDGAKETCAALLFNLST--QDEVKTELVLKGGIRLAFALWEGASPEARRSCSLVACNLAVGKVNTAKMVRHGAGQLLVELVKNTDPLNDAGG 2724
            +++ G  PA+++LAA    +  +  C +A   LA VP +   ++    V  I L V+ A+ S     L  + G + E+  LE  L+     ++L  L    N + K LV      +   +++    +DV   C +++C       + P  +     QAL NLT+ +     +  E  H +  L                                  AA  + R+ I+Q G+IP +V       L       + +    LS D    + +V   A  A++       S    + +  L +L         L++ GVV  L++++ D  DE      +    L  C   +  AL     +I  L+ L +    S+    + A++N+   + +     + +  + + L  LL     + L       +Y LA               V+GA+      A+  + +T  Q                S+++ +   +   + I  +S V  L  ++   ++RD   +   AV     C                         E+++ E A   LI+L    N+ +++ C  +   + A+ +  E ++ + GA++  +I ALVA+ SD+ +  C K ++N L  +  H  R M   GV W L+++S          C+   CNLS  Y  ++  +   +A+  +  + D   +   ++A+ N LL      G+  V  G V I RR +   ++ +     +V ++ +       V    + L   +  V   D  + +SL     C     A   + P     +L +       A+ +     MS   Q LY+++C P  +  LV     + +   V     H  D  +                              L+NL+    +   + LV  G  RL    +       +  C L  CNLA+GKVN++++V    G +LV+ V++T   ND+ G
Sbjct:  157 LLDNGIVPALVSLAAVPDTQT-QLHCAKAFYGLAQVPCTRRLLVLNNIVGTISLLVRDAIRSKFKQDLAAIVGHITEESFLEDKLLHDGVDRVLVKLYHGHNAETKRLVALSFFNLSHNSSQLKHFIDVFTQCITSVCKTMSPA-SHPSKATLLLLQALFNLTQTQSFHNALMAENVHRVLSLPLAHFCKCHQHKIRPTESVEDDSIEFGLKGLFMLAATKNGRQHIVQDGLIPNIVACLV---LGGVVADTVCIILYQLSMDETCRDAVVHDLA--AVVDNIVHCSSYGYFTVSWVLRHLCATKANFPALVDAGVVPVLMQMSRDSHDEVKLNGISCMCCLLQCDLRLNNALTCMERIILDLVQLLQSDVESIVVFAITALFNLACNDTLTPFLSAPSTGVVLSLKDLLHRHPSEKLTTVLLPLVYQLAPTTRALMLQSGFFDFVAGAISTTTGPARHAALDTMVQFTLEAEAFPQGTEDVRSLVKALYTLKTNNDTIALRSCVSLLAHLTTSPKNRDLLLKTGCAVCLARICNTADDFIMANCAYILFCFTETLDAVEVIVREEAIPVLIQLSRASNDTVKELCILSFCRISAHGNNMETKLVEQGAIAAAMIMALVATKSDQIKTLCVKIISNCLSLQAKHCTRTMIDHGVFWALSSLSTLPFADTKHACATCFCNLSAAYPLKMVEAGVPRALVQVVETGDGPTIVIALEAIAN-LLHNDKACGI-LVNEGIVKILRRLVEDTNETVSHAAAMVLLKTSRADDRCRVESLRSGLVPWMRSVLHNDVLATQSLITLCDCTANAAAQGHVHPLHVIDILRA-------AVPSSHPHRMSLCLQALYNMTCQPSKLLELVHAGAHLFLQQGV-----HTDDTTLS--------------------------VMSLYNLTCVLDEGALSALVASGLTRLLHDTY--LLDIEKPWCVLSICNLALGKVNSSRIVMENGGVVLVDFVQSTAFTNDSSG 1103          

BLAST of mRNA_E-fasciculatus_F_contig1274.2084.1 vs. uniprot
Match: A0A1V9ZVE9_9STRA (Vacuolar protein 8 n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZVE9_9STRA)

HSP 1 Score: 89.7 bits (221), Expect = 4.340e-14
Identity = 134/604 (22.19%), Postives = 253/604 (41.89%), Query Frame = 1

Query:   52 IEEGAAPAIIALAASSHNRYLR-SQCVEALCKLAVVPGSEAQIIAEGGVSCILAVQRAVPSLGELAGTVEEDESLERLLIQLLCNLSGFKNNQLKLVEEGAIRIISRAAERTASVDVVRLCASTLCNFAGEGRARPKMSDSRTAQALLNLT--KHEDVGIRREVAHTIARLAADASCREKILQYGIIPILVTMSTAPDLDTTTGR-CIALAFRVLSSDRKFAEIIVDGGAVDALISLTRSTDSPCRLSCAQSLCNLIRFAIRLDYLIECGVVSALVRLADPGDEATSECCAAALYLFFCHPAVLALIDGREVIRALMDLCRVGTTSVRKRCVAAIWNMTNVEQVAHSGGSAAESIPMLLGLLQTESDKDLQADCAAALYNLARNQENCQAMIVSGAVPPIIVLAKSGSFETKTQCMSILQRM--------LLGRAMPEEIMTKSFVRTLLDMSKMEHRDTQQRVVIAVYRISCCERGRELLLEEGAPESLIRLISKPNEEMRKGCANT-LLNLAYDHGR-EIEITKCGAVSVLLITALVASDSDETRHACTKALTNLLCEKQAHRGMFKDGVVWGLAAMSRSKDVTIAKLCSVALCNLSCEYWK 1821
            +E+GA  A+  L +  + +  + + CV  +C LA +  ++  +I  G +  I+ +   +P       + E   + +          + F   + KL++   I  ++   +  A +++   CA TL  F+   + +P++  +     LL L   +HE+    + +  +++  A+  SCR ++++ G +  L+T+ +AP  D    + C+     ++     + E+ VD G V AL++ +       + SCA +L +L         L++ GV++AL+ LAD   +   E  AA L          AL      +++   L   GT  +    VA +    N +Q   S      S+ +L  +   E +++   D  A    L   Q N        A      LA++ ++ +   C      M        LL  A    I   S  R   D++ +    T  R  +A   +SC  +GR L+ + G     +  + +      + CA   L NLA + G  + ++     VS  ++ AL+  +SD T+  C K L NLL        +  DG ++ L  +++     I +LC  ++ N+S +  K
Sbjct:  391 VEDGAILALKILCSYRNVQVCQLASCV--VCNLAALRANQDNMIKNGALLVIMELFN-LPQFHRKQTSPETQNTFD---------YTRFYAKKAKLLDPNYIGDLAFTDDENAMLEIHMQCAITLAYFSCNSKLQPRVVGAGFVPRLLALLGMRHEETT--KYITLSLSNFASHESCRVQMVREGAVRPLITLMSAPYADMLVKQDCVVTLCNLMLHPETYKEM-VDDGVVPALVAYSEDNHPEIQKSCAFALLSLTMDTSMKPKLVQQGVITALISLADRCAQ-RPELRAACL---------CALYHISTDVQSAQALYYEGTQFL---AVAVLNEPLNDKQPNISHRMYMHSLALLSNMASYEKERNALVDDGAVDAVLRFLQANSTVKDKVSAQ----YLARAQAYASSLLCKLSEVAMEHPGYFSALLVLASSANITPNSSNRN--DVTGII---TTLRCALAFANMSCSMKGRRLMGKHGEVAIGLNAMMRTGHHETQICAAIGLCNLAIERGPLKGQVWADNTVSDFIVVALLRVNSDTTKVICAKVLFNLLAHDDTREKLIVDGALYALIKLAKLDMEEIRQLCLRSIYNISLDLGK 957