mRNA_E-fasciculatus_F_contig1272.2071.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

BLAST of mRNA_E-fasciculatus_F_contig1272.2071.1 vs. uniprot
Match: D7G0V0_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0V0_ECTSI)

HSP 1 Score: 446 bits (1147), Expect = 3.340e-153
Identity = 260/366 (71.04%), Postives = 275/366 (75.14%), Query Frame = 1

Query:   10 GANRPGLSGGHDGVSQSSRNRTCLFPGCNARPEYGLPGATRAVYCFSHKKGPMVQLLKEPPALPNNAGKAESSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGTKKTRAPSNTDRNGAKKARRGLQVREXXXXXXXXXXXXXXXXXXKGGNDERDAISSATVTGENLVKTTAKATVSSLPSPAPAAE---PSSPHNRLADSSGMAPSVEFLLLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWLSREKRAEDLRFKAELRQQRKQYQAAGLKSGVVLEMFVPAATGTEAAGDGIEERVGEVRGAGAATTPRGGNGNPGWQKVSPEVLSAPSAKRDGTGQRKGTSAGR 1098
            GANRPGLSGGH+G SQSSRNR+CLFPGC+ARP+YGLPGATRAVYCFSHKKGPMVQL KE PALP+N GKA +                                     VGTK  RAPSN DRN AKKARRG Q  E                   GG DER AISS+TV GENL KTTAK +  S PSPAPAAE   PSSP+NRLADSSGMAPSVEFL+LRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQW+SREK+AEDLR KAELRQQRKQYQAAGLKSGVVLEMFVP ATGTEAAGDGIEER GEVRG G ATT RG N  PGWQKVSPEVLSAPS+K+ GTGQR+ TS GR
Sbjct:   31 GANRPGLSGGHNGGSQSSRNRSCLFPGCDARPDYGLPGATRAVYCFSHKKGPMVQLFKETPALPDNPGKATTGKPRSQAPTEPAAAAAS--------------------VGTKNPRAPSNIDRNAAKKARRGQQETEEDNDADVSGTHRSKKRS-SGGKDERGAISSSTVAGENLTKTTAKTSAPSFPSPAPAAEANPPSSPNNRLADSSGMAPSVEFLVLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWMSREKKAEDLRLKAELRQQRKQYQAAGLKSGVVLEMFVPEATGTEAAGDGIEERDGEVRGEGGATTLRGSN--PGWQKVSPEVLSAPSSKQHGTGQREETSVGR 373          

BLAST of mRNA_E-fasciculatus_F_contig1272.2071.1 vs. uniprot
Match: A0A6H5L360_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L360_9PHAE)

HSP 1 Score: 426 bits (1095), Expect = 5.990e-142
Identity = 255/369 (69.11%), Postives = 275/369 (74.53%), Query Frame = 1

Query:    1 LISGANRPGLSGGHDGVSQSSRNRTCLFPGCNARPEYGLPGATRAVYCFSHKKGPMVQLLKEPPALPNNAGKAESSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGTKKTRAPSNTDRNGAKKARRGLQVREXXXXXXXXXXXXXXXXXXKGGNDERDAISSATVTGENLVKTTAKATVSSLPSPAPAAEPSSP---HNRLADSSGMAPSVEFLLLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWLSREKRAEDLRFKAELRQQRKQYQAAGLKSGVVLEMFVPAATGTEAAGDGIEERVGEVRGAGAATTPRGGNGNPGWQKVSPEVLSAPSAKRDGTGQRKGTSAGR 1098
            ++  ANR G+SGGH+G SQSSRNRTCLFPGC+ARP+YGLPGATRA+YCF+HKK PMVQL KE PALPN AG A +         XXXXXXXX                    VG  KTRAPSN DRNGAKKARRG Q  E                   GG DER  ISSA VTGENL K T K + SSLP PAPA EP+ P   +NRLADSSGMAPSVEFL+LRQARE AKTEAEASGLGRRPRAPSRRALEAAGRLKG GAQW+SREK+AEDLR KAELR QRKQYQAAGLKSGVVLEMFVPAATGTEAAGDGIE+R GEVRG G ATTPRG N  PGW KVSPE LSAPS+K+ G+GQ KG+S GR
Sbjct:  265 VLKRANRQGISGGHNGGSQSSRNRTCLFPGCDARPDYGLPGATRAMYCFAHKKAPMVQLFKELPALPNKAGNATTGKRSQGPAAXXXXXXXXS-------------------VGANKTRAPSNIDRNGAKKARRGPQETEEDNDVDSGGKHTSKKRRSTGGKDERGGISSAKVTGENLTKPTVKTSASSLPPPAPAFEPNPPSSSNNRLADSSGMAPSVEFLVLRQAREAAKTEAEASGLGRRPRAPSRRALEAAGRLKGIGAQWMSREKKAEDLRLKAELRLQRKQYQAAGLKSGVVLEMFVPAATGTEAAGDGIEQRDGEVRGEGGATTPRGSN--PGWHKVSPEALSAPSSKQHGSGQGKGSSVGR 612          

BLAST of mRNA_E-fasciculatus_F_contig1272.2071.1 vs. uniprot
Match: A0A6H5LLY6_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LLY6_9PHAE)

HSP 1 Score: 421 bits (1083), Expect = 3.740e-140
Identity = 265/368 (72.01%), Postives = 284/368 (77.17%), Query Frame = 1

Query:    1 LISGANRPGLSGGHDGVSQSSRNRTCLFPGCNARPEYGLPGATRAVYCFSHKKGPMVQLLKEPPALPNNAGKAESSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGTKKTRAPSNTDRNGAKKARRGLQVREXXXXXXXXXXXXXXXXXXKGGNDERDAISSATVTGENLVKTTAKATVSSLPSPAPAAEP---SSPHNRLADSSGMAPSVEFLLLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWLSREKRAEDLRFKAELRQQRKQYQAAGLKSGVVLEMFVPAATGTEAAGDGIEERVGEVRGAGAATTPRGGNGNPGWQKVSPEVLSAPSAKRDGTGQRKGTSAG 1095
            ++  ANRPGLSGG++  SQSSR+  CLFPGC+ARP+YGLPGATRAVYCFSHKKGPMVQL KE PALP++AGKA +            XXXXXXXXXXXXXXXX          G KK RAP N DRNGAK ARRG Q  E                   GG DER AISS+TVTGENL KTTAK   SSLPSPAPAAEP   S P+NRLADSSGMAPSVEFL+LRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQW+SREK+AEDLR KAELR QRK+YQAAGLKSGVVLEMFVPAATGTEAAGDGIEER GEVRG G ATTP G N  PGWQKVSPE LSA S K+  TGQR+ +S G
Sbjct:  255 VLKRANRPGLSGGNNRESQSSRDSACLFPGCDARPDYGLPGATRAVYCFSHKKGPMVQLFKETPALPDSAGKATTGKPRSQVPTESSXXXXXXXXXXXXXXXX---------XGAKKKRAPRNIDRNGAKNARRGRQETEEDNNADGSGTHKSEKRS-SGGKDERGAISSSTVTGENLTKTTAKPAASSLPSPAPAAEPKPPSFPNNRLADSSGMAPSVEFLVLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWMSREKKAEDLRLKAELRLQRKRYQAAGLKSGVVLEMFVPAATGTEAAGDGIEERDGEVRGEGGATTPIGSN--PGWQKVSPETLSAASPKQHSTGQRRESSVG 610          

BLAST of mRNA_E-fasciculatus_F_contig1272.2071.1 vs. uniprot
Match: D7G0U7_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0U7_ECTSI)

HSP 1 Score: 307 bits (787), Expect = 8.800e-99
Identity = 209/361 (57.89%), Postives = 222/361 (61.50%), Query Frame = 1

Query:   25 GLSGGHDGVSQSSRNRTCLFPGCNARPEYGLPGATRAVYCFSHKKGPMVQLLKEPPALPNNAGKAESSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGTKKTRAPSNTDRNGAKKARRGLQVREXXXXXXXXXXXXXXXXXXKGGNDERDAISSATVTGENLVKTTAKATVSSLPSPAPAAE---PSSPHNRLADSSGMAPSVEFLLLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWLSREKRAEDLRFKAELRQQRKQYQAAGLKSGVVLEMFVPAATGTEAAGDGIEERVGEVRGAGAATTPRGGNGNPGWQKVSPEVLSAPSAKRDGTGQRKGTSAGR 1098
            G SG     S     RT    GC ARP+YGLPGATRAVYCFSHKKGPMVQL KEPP+LPN AG+AES+A                  XXXXXXXX         VG KKTRAPS+ DRNG+KKARRG Q  E                   GG DER AISSA VT ENL KTTAK +  SLPSPAPAAE   PSSP+NRLADSSGMAPSVEFL+LRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQW+SREK+AEDLR KAELRQQRKQY                                                     QKVSPE LSAP +K+ GTGQRK TS GR
Sbjct:   70 GSSGRSSSSSHPMPGRTSATRGCEARPDYGLPGATRAVYCFSHKKGPMVQLFKEPPSLPNKAGQAESAAAPVVTRKATTGKRSQGPAXXXXXXXXS--------VGAKKTRAPSDIDRNGSKKARRGQQETEEDNDADVSGTHRSKKRR-SGGKDERGAISSAQVTKENLTKTTAKTSAPSLPSPAPAAEANPPSSPNNRLADSSGMAPSVEFLVLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWMSREKKAEDLRVKAELRQQRKQY-----------------------------------------------------QKVSPEALSAPYSKQHGTGQRKETSVGR 368          

BLAST of mRNA_E-fasciculatus_F_contig1272.2071.1 vs. uniprot
Match: D8LEM4_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LEM4_ECTSI)

HSP 1 Score: 195 bits (496), Expect = 2.110e-55
Identity = 136/265 (51.32%), Postives = 165/265 (62.26%), Query Frame = 1

Query:  355 RAPSNTDRNGAKKARRGLQVREXXXXXXXXXXXXXXXXXX--KGGNDERDAISSATVTGENLVKTTA-KATVSSLPSPAPAAEP---SSPHNRLADSSGMAPSVEFLLLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWLSREKRAEDLRFKAELRQQRKQYQAAGLKSGVVLEMFVPAATGTEAAGDGIEERVGEVRGAGAATTPRGGNGNPGWQKVSPEVLSAPSAKRDGTGQRKGTSAGRDRSL*SKPGPP 1131
            RAPSN D+ GA KA +G +  +                     GG D R  +S    T E+L  T + + TVSSL SP  AA     SSP NR  D++GMAPSVEF LLRQARE AK EA ASG GRRPRAPSRRALEA+G+LK  GAQW++RE++AE+ RFKAELR+QR+QY+AAGLKSGVVLE FV  A GT  AGDG+   V    G G+ATT  G +  P WQKVSP +L+A   +   T Q +  S   +R    +P PP
Sbjct:   80 RAPSNVDKTGANKAWQGRKATKDRDSTDGGDKNSSSKKRRGSNGGKDNRGEVSKVNATREDLPTTVSVETTVSSLRSPTSAAATKPLSSPPNRSGDTAGMAPSVEFHLLRQAREVAKKEAAASGGGRRPRAPSRRALEASGQLKHDGAQWMTRERKAEEARFKAELREQRRQYKAAGLKSGVVLEAFVAEAAGTGTAGDGVGGGVAGELGVGSATTKHGRD--PAWQKVSPGILAASPEQEQATEQEETLSPSSERPS-KRPRPP 341          

BLAST of mRNA_E-fasciculatus_F_contig1272.2071.1 vs. uniprot
Match: A0A6H5L640_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L640_9PHAE)

HSP 1 Score: 190 bits (483), Expect = 4.800e-54
Identity = 131/266 (49.25%), Postives = 163/266 (61.28%), Query Frame = 1

Query:  352 TRAPSNTDRNGAKKARRGLQVREXXXXXXXXXXXXXXXXXX--KGGNDERDAISSATVTGENLVKTTA-KATVSSLPSPAPAAEP---SSPHNRLADSSGMAPSVEFLLLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWLSREKRAEDLRFKAELRQQRKQYQAAGLKSGVVLEMFVPAATGTEAAGDGIEERVGEVRGAGAATTPRGGNGNPGWQKVSPEVLSAPSAKRDGTGQRKGTSAGRDRSL*SKPGPP 1131
            TRAPS+ D+ GA  A +  + +                      GG D R   S    T E+L  T + + TVSSLPSP  AA     SSP NR  D++GMAPSVEF LLRQARE AK EA  SG GRRPRAPSRRALEA+G+LK  GAQW++RE++AE+ R KAELR+QR+QY+AAG KSGVVLE FV  A GT   GDG+   V    G G+ATT  G +  P WQKVSPE+L+A + +   T + +  SA  ++    +P PP
Sbjct:   29 TRAPSSVDKTGANNAWQSRKAKNDGYSTDGGDKNSSSKKRRGSNGGKDNRGEASKGNATREDLPTTVSVETTVSSLPSPTSAAATKPLSSPPNRSGDTAGMAPSVEFHLLRQAREAAKKEAAXSGGGRRPRAPSRRALEASGQLKHDGAQWMTRERKAEEARIKAELREQRRQYKAAGFKSGVVLEAFVAKAAGTGTVGDGVGGGVAGKIGVGSATTKHGRD--PAWQKVSPEMLAASAEQEQATEKEELLSASAEKRPSKRPRPP 292          

BLAST of mRNA_E-fasciculatus_F_contig1272.2071.1 vs. uniprot
Match: A0A6H5KLI4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KLI4_9PHAE)

HSP 1 Score: 191 bits (485), Expect = 1.800e-51
Identity = 145/341 (42.52%), Postives = 177/341 (51.91%), Query Frame = 1

Query:   16 NRPGLSGGHDGVSQSSRNRTCLFPGCNARPEYGLPGATRAVYCFSHKKGPMVQLLKEPPALPNNAGKAESSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGTKKTRAPSN--TDRNGAKKARRGLQVREXXXXXXXXXXXXXXXXXX--------------KGGNDERDAISSATVTGENLVKTTA-----KATVSSLPSPAPAAEPSSPHNRLADSSGMAPSVEFLLLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWLSREKRAEDLRFKAELRQQRKQYQAAGLKSGVVLEMFVPAATGTEAAGDGIEERVGEVRGAGA 975
            NRPG S      S + +NR+CLFPGC ARP +GLPGA RAVYCFSHKKG MV L  E  A+ N+  K+ +SA                                      KK R  +    D+ GAK+ARR   + E                                 GG+    A ++A  T E  VK+ A     K+T  +       A P  P +R +D  GMAPSVE L+LRQARE AKTEAEASG GRRPRAPSRRALEA+GRL+G GAQW++REK+AE  R  +ELR++RK+ + AGLK GVVLE FVP A    A      E  G +RG GA
Sbjct:  229 NRPGTSSSRHEESPAPKNRSCLFPGCGARPTHGLPGAARAVYCFSHKKGSMVDLFSEALAVNNHEEKSVTSAAPTEGKKKKQTQ-------------------------AKKEREMTGMTADQRGAKQARRESSIMEGNKGTTSKDDGGHRGAKKIRDNETKVNRGMGASGGSVGAIATAAAAGTSEMSVKSPAAKPLVKSTSEAKEPADTTAPPPQPTSRPSD--GMAPSVEMLILRQAREAAKTEAEASGSGRRPRAPSRRALEASGRLRGDGAQWMTREKKAEQARVNSELRKKRKESREAGLKVGVVLEAFVPRAPSRRAL-----EASGRLRGDGA 537          

BLAST of mRNA_E-fasciculatus_F_contig1272.2071.1 vs. uniprot
Match: D8LH07_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LH07_ECTSI)

HSP 1 Score: 181 bits (460), Expect = 3.450e-50
Identity = 140/314 (44.59%), Postives = 168/314 (53.50%), Query Frame = 1

Query:   16 NRPGLSGGHDGVSQSSRNRTCLFPGCNARPEYGLPGATRAVYCFSHKKGPMVQLLKEPPALPNNAGKAESSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGTKKTRAPSNTDRNGAKKARRGLQVREXXXXXXXXXXXXXXXXXXKGGNDERDAISSATVTGENLV---KTTAKATVSSLPSPAPAAEP--------SSPHNRLAD--------SSGMAPSVEFLLLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWLSREKRAEDLRFKAELRQQRKQYQAAGLKSGVVLEMFV 900
            NRPG S      S + +NR CLFPGC ARP YGLPGA  AVYCFSHKKG MV L    PA+ N+  K  + A        XXXXXXXX                       +       D+ GAKKARR   ++E                    GN+ +  ++  T   E  V    T A A  S + +  PAA+P          P + +A         S GMAPSVE L+LRQARE AKTEAEASG GRR RAPSRRALEA+G+LKG GAQW++REK+AE  R   ELR++RK+ + AGLK GVVLE FV
Sbjct:   75 NRPGTSSSGHEESPAPQNRPCLFPGCGARPIYGLPGAAHAVYCFSHKKGSMVNLFSRAPAVNNHEEKMVTPAAPTEGKRKXXXXXXXX-----------------------RKMTGMTADQRGAKKARRESSIKEDNKGATSKDDGGHRGAKKNRGNETK--VNRGTGASEGSVGDVATAAAAGTSEMSTKLPAAKPLVKLTSKAEEPADTMAPPPQPTSRPSEGMAPSVEMLILRQAREAAKTEAEASGGGRRARAPSRRALEASGQLKGDGAQWMTREKKAEQARVNTELREKRKKSREAGLKVGVVLEAFV 363          

BLAST of mRNA_E-fasciculatus_F_contig1272.2071.1 vs. uniprot
Match: A0A6H5KYF0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KYF0_9PHAE)

HSP 1 Score: 179 bits (455), Expect = 1.820e-48
Identity = 128/313 (40.89%), Postives = 159/313 (50.80%), Query Frame = 1

Query:   19 RPGLSGGHDGVSQSSRNRTCLFPGCNARPEYGLPGATRAVYCFSHKKGPMVQLLKEPPALPNNAGKAESSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGTKKTRAPSNTDRNGAKKARRGLQVREXXXXXXXXXXXXXXXXXXKGGNDERDAISSATVTGE---NLVKTTAKATVSSLPSPAPAAEPS----------------SPHNRLADSSGMAPSVEFLLLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWLSREKRAEDLRFKAELRQQRKQYQAAGLKSGVVLEMFV 900
            RPG S      S++ +  +CLFP C ARP YGLPGA RA YCFSHKKG MV L  E PA+ N+  K  + A                                      ++       D+ GAKKARR   ++E                  K   D +  ++      E     + T A A  S + + +PAA+PS                 P      S GMAPSVE L+LRQARE AKTEAEASG GRRPRAPSRRALEA+GRL+G GAQW++REK+AE  R  +ELR++RK+ + AGLK GVVLE FV
Sbjct:  176 RPGTSSSRHEESRAPQYHSCLFPECGARPIYGLPGAARAAYCFSHKKGSMVDLFSEAPAVNNHEAKRVTPAAPTEGKRKKQKKGHE-----------------------ERKMTGMTADQRGAKKARRESSIKEDNKGATSKDDGGHRGA--KKIRDNKTKVNGGMGASEASVGAIVTAAAAGTSEMSTKSPAAKPSVKPTRKVKEPADTIVPPPQPTSRPSDGMAPSVEMLILRQAREAAKTEAEASGSGRRPRAPSRRALEASGRLRGDGAQWMTREKKAEQARVNSELRKKRKESREAGLKVGVVLEAFV 463          

BLAST of mRNA_E-fasciculatus_F_contig1272.2071.1 vs. uniprot
Match: D8LGW8_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LGW8_ECTSI)

HSP 1 Score: 177 bits (448), Expect = 1.890e-47
Identity = 140/314 (44.59%), Postives = 171/314 (54.46%), Query Frame = 1

Query:   16 NRPGLSGGHDGVSQSSRNRTCLFPGCNARPEYGLPGATRAVYCFSHKKGPMVQLLKEPPALPNNAGKAESSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGTKKTRAPSNTDRNGAKKARRGLQVREXXXXXXXXXXXXXXXXXXKGGNDER------------DAISSATVTGENLVKTTAKATVSSLPSPAPAAEPSS-------PHNRLADSSGMAPSVEFLLLRQAREDAKTEAEASGLGRRPRAPSRRALEAAGRLKGTGAQWLSREKRAEDLRFKAELRQQRKQYQAAGLKSGVVLEMFV 900
            NRP  S      S + +NR+CLFPGC+ARP YGLPGA RAVYCFSHKKG MV L  + PA+ NN  K  ++          XXXXXXXXXXX                           D+ GAKKARR   ++E                     N+++             AI++A   G + + T + A    + S   A EP+        P NR   S GM+PSVE L+LRQARE AK EAEASG GRR RAPSRRALEA+GRL+G GAQW++REKRAE  R   ELR++RK+ + AGL  GVVLE FV
Sbjct:  175 NRPEPSSSGHEESPALQNRSCLFPGCDARPTYGLPGAARAVYCFSHKKGSMVNLFSQAPAVNNNEEKRVTAVAPTEGKRKKXXXXXXXXXXXSM-----------------------TADQRGAKKARRESSIKEDNKGATSKDGGGYQGAKKIRDNEKKVNRGVGTSERSVGAIATAAAAGTSEMSTKSSAAKPPVKSTHIAKEPADTMALPPQPTNR--PSEGMSPSVEMLILRQAREAAKKEAEASGGGRRARAPSRRALEASGRLRGDGAQWMTREKRAEQARVNTELREKRKKSREAGLTVGVVLEAFV 463