mRNA_E-fasciculatus_F_contig12526.1942.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig12526.1942.1
Unique NamemRNA_E-fasciculatus_F_contig12526.1942.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig12526.1942.1 vs. uniprot
Match: D7FHB8_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FHB8_ECTSI)

HSP 1 Score: 186 bits (472), Expect = 1.080e-52
Identity = 91/92 (98.91%), Postives = 92/92 (100.00%), Query Frame = 1 
Query:    4 QILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRFQ 279
            +ILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRFQ
Sbjct:  664 KILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRFQ 755
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BLAST of mRNA_E-fasciculatus_F_contig12526.1942.1 vs. uniprot
Match: A0A7R9YDF9_9STRA (Hypothetical protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9YDF9_9STRA)

HSP 1 Score: 89.0 bits (219), Expect = 1.520e-18
Identity = 46/93 (49.46%), Postives = 60/93 (64.52%), Query Frame = 1 
Query:    1 VQILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRFQ 279
            +++ A+ VYR LG+F  A+  + EL VKQIQMDTLSY+++   L  G F E Q QC  ++ +HRS   D AEH A A  RGNY +G EV  FQ
Sbjct:  633 IRLQALLVYRALGAFLPAIEHYNELQVKQIQMDTLSYVIVPGLLRLGFFNEAQQQCDRVSRMHRSAARDAAEHCAKAVVRGNYLQGTEVMTFQ 725
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BLAST of mRNA_E-fasciculatus_F_contig12526.1942.1 vs. uniprot
Match: A0A7S2XYA5_9STRA (Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XYA5_9STRA)

HSP 1 Score: 73.6 bits (179), Expect = 2.080e-13
Identity = 36/92 (39.13%), Postives = 52/92 (56.52%), Query Frame = 1 
Query:    4 QILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRFQ 279
            ++L I VYR +G+F   L +  +L++K IQ+D+LS+L+    +  G F E    CKGI   HR C  D A+ +  A   GNY K  E+  FQ
Sbjct:  178 KLLLIQVYRAMGAFEPGLVLHNQLEIKSIQLDSLSFLLFPGMMRCGFFNEASQLCKGIIQFHRKCALDCADFLKDASHVGNYVKAIELVEFQ 269
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BLAST of mRNA_E-fasciculatus_F_contig12526.1942.1 vs. uniprot
Match: A0A7S1U7H7_9STRA (Hypothetical protein (Fragment) n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1U7H7_9STRA)

HSP 1 Score: 69.7 bits (169), Expect = 8.710e-13
Identity = 36/89 (40.45%), Postives = 49/89 (55.06%), Query Frame = 1 
Query:   13 AIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRFQ 279
            A+   R L ++A A+  F  L VK +QMDTLSYL+L   L  G F E   QC+ +  +HR    + +E  A A   GNY + AE+  FQ
Sbjct:   75 ALLAQRALAAYAPAIATFNSLQVKHVQMDTLSYLLLPSLLRLGFFSEAMLQCEHVKRMHRGAAREASEWSAKAIALGNYMQAAEIVHFQ 163
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BLAST of mRNA_E-fasciculatus_F_contig12526.1942.1 vs. uniprot
Match: A0A6G0XIP9_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XIP9_9STRA)

HSP 1 Score: 72.4 bits (176), Expect = 9.800e-13
Identity = 36/91 (39.56%), Postives = 53/91 (58.24%), Query Frame = 1 
Query:    4 QILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRF 276
            ++L   +Y +LG+ +     + ELD+KQIQMD+LSYL+L P L  G F E    C  + A+HR+   DT E +A A+  G  SK  ++  F
Sbjct:  516 KLLLCRIYARLGAGSAMFARYQELDIKQIQMDSLSYLILDPLLALGQFDEAVATCDAVRALHRTTARDTPEFIARAYRLGVLSKAQDMTSF 606
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BLAST of mRNA_E-fasciculatus_F_contig12526.1942.1 vs. uniprot
Match: A0A024U7B8_9STRA (Uncharacterized protein n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024U7B8_9STRA)

HSP 1 Score: 66.2 bits (160), Expect = 1.430e-10
Identity = 34/91 (37.36%), Postives = 54/91 (59.34%), Query Frame = 1 
Query:    4 QILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRF 276
            ++L   VY  LG+     T + ELD+KQIQ+D+LSYL+L P L  G   + +  C+ I ++HR+   DT E ++ A+  G +SK  ++  F
Sbjct:  533 KLLLCRVYALLGAGDAFFTRYHELDIKQIQVDSLSYLVLDPLLALGQVDDARRICESIRSLHRTTARDTPEFISRAYRLGVFSKAQDMTGF 623
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BLAST of mRNA_E-fasciculatus_F_contig12526.1942.1 vs. uniprot
Match: A0A1V9ZJE1_9STRA (Uncharacterized protein n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9ZJE1_9STRA)

HSP 1 Score: 65.1 bits (157), Expect = 3.640e-10
Identity = 36/93 (38.71%), Postives = 53/93 (56.99%), Query Frame = 1 
Query:    4 QILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRFQV 282
            ++L   VY  LG+ A  LT + ELDVKQIQ+D+LS+L+L      GL  E    C  + A+H++   DT E +  A+  G +SK  ++  F V
Sbjct:  506 KLLLCRVYALLGAGAAVLTRYKELDVKQIQLDSLSHLVLDGLFALGLDEEALRICDAVRALHQTTARDTPEFIGRAYRLGVFSKAEDMTSFLV 598
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BLAST of mRNA_E-fasciculatus_F_contig12526.1942.1 vs. uniprot
Match: W4GD98_9STRA (Uncharacterized protein n=9 Tax=Aphanomyces astaci TaxID=112090 RepID=W4GD98_9STRA)

HSP 1 Score: 65.1 bits (157), Expect = 3.650e-10
Identity = 33/91 (36.26%), Postives = 53/91 (58.24%), Query Frame = 1 
Query:    4 QILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRF 276
            ++L   +Y  LG+       + ELDVKQIQ+D+LSYL+L P L  G   + +  C  + A+HR+   DT E ++ A++ G +SK  ++  F
Sbjct:  524 KLLLCRIYALLGAGTAFFGRYQELDVKQIQVDSLSYLVLDPLLALGQVDDARRICDAVRALHRTTARDTPEFISRAYKLGVFSKAQDMTMF 614
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BLAST of mRNA_E-fasciculatus_F_contig12526.1942.1 vs. uniprot
Match: A0A485L2N3_9STRA (Aste57867_14856 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485L2N3_9STRA)

HSP 1 Score: 64.3 bits (155), Expect = 6.670e-10
Identity = 35/91 (38.46%), Postives = 52/91 (57.14%), Query Frame = 1 
Query:    4 QILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRF 276
            ++L   VY  LG+    +  + ELDVKQIQ+D+LSYL++ P L  G     +  C  I ++HRS   DT E +A A+  G +SK  ++  F
Sbjct:  272 KMLLCRVYALLGAGDAVVARYDELDVKQIQLDSLSYLIVDPLLALGHVEYAKTICDNIRSLHRSTARDTPEFIARAYRLGVFSKAQDMTGF 362
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BLAST of mRNA_E-fasciculatus_F_contig12526.1942.1 vs. uniprot
Match: T0QBN7_SAPDV (Uncharacterized protein n=1 Tax=Saprolegnia diclina (strain VS20) TaxID=1156394 RepID=T0QBN7_SAPDV)

HSP 1 Score: 64.3 bits (155), Expect = 6.790e-10
Identity = 37/101 (36.63%), Postives = 57/101 (56.44%), Query Frame = 1 
Query:    4 QILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFGEVQGQ-----CKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRFQVCLV 291
            ++L   VY  LG+    L+ + ELD+KQIQ+D+LSYL+L      GLFG    +     C+ I A+H++   DT E +  A+  G YSK  ++  F +C +
Sbjct:  499 KMLLCRVYALLGAGDAVLSRYKELDIKQIQLDSLSYLVLD-----GLFGLQMDKDGLRLCESIRALHKTTSRDTPEFIGRAYRLGVYSKAQDMTSFLLCKM 594
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig12526.1942.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FHB8_ECTSI1.080e-5298.91Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A7R9YDF9_9STRA1.520e-1849.46Hypothetical protein n=1 Tax=Pinguiococcus pyrenoi... [more]
A0A7S2XYA5_9STRA2.080e-1339.13Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa... [more]
A0A7S1U7H7_9STRA8.710e-1340.45Hypothetical protein (Fragment) n=1 Tax=Phaeomonas... [more]
A0A6G0XIP9_9STRA9.800e-1339.56Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
A0A024U7B8_9STRA1.430e-1037.36Uncharacterized protein n=2 Tax=Aphanomyces invada... [more]
A0A1V9ZJE1_9STRA3.640e-1038.71Uncharacterized protein n=1 Tax=Achlya hypogyna Ta... [more]
W4GD98_9STRA3.650e-1036.26Uncharacterized protein n=9 Tax=Aphanomyces astaci... [more]
A0A485L2N3_9STRA6.670e-1038.46Aste57867_14856 protein n=1 Tax=Aphanomyces stella... [more]
T0QBN7_SAPDV6.790e-1036.63Uncharacterized protein n=1 Tax=Saprolegnia diclin... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig12526contigE-fasciculatus_F_contig12526:2644..3474 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score188.0
Seed ortholog evalue2.2e-45
Seed eggNOG ortholog2880.D7FHB8
Preferred nameNAA25
KEGG koko:K17973
GOsGO:0000001,GO:0000003,GO:0000278,GO:0000281,GO:0000323,GO:0000902,GO:0000904,GO:0000910,GO:0000912,GO:0000915,GO:0001667,GO:0002064,GO:0002065,GO:0002066,GO:0002253,GO:0002376,GO:0002682,GO:0002684,GO:0003006,GO:0003674,GO:0003779,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005773,GO:0005794,GO:0005829,GO:0006464,GO:0006473,GO:0006474,GO:0006807,GO:0006810,GO:0006897,GO:0006909,GO:0006911,GO:0006928,GO:0006935,GO:0006955,GO:0006996,GO:0007005,GO:0007010,GO:0007015,GO:0007049,GO:0007275,GO:0007276,GO:0007281,GO:0007292,GO:0007297,GO:0007298,GO:0007399,GO:0007409,GO:0007411,GO:0008092,GO:0008150,GO:0008152,GO:0008154,GO:0009605,GO:0009653,GO:0009888,GO:0009987,GO:0010324,GO:0010467,GO:0010591,GO:0010592,GO:0010631,GO:0010941,GO:0012505,GO:0016043,GO:0016192,GO:0016477,GO:0017196,GO:0018193,GO:0018206,GO:0019538,GO:0019953,GO:0022008,GO:0022402,GO:0022411,GO:0022412,GO:0022414,GO:0022607,GO:0030027,GO:0030029,GO:0030030,GO:0030036,GO:0030042,GO:0030154,GO:0030182,GO:0030424,GO:0030426,GO:0030427,GO:0030707,GO:0030855,GO:0030865,GO:0030866,GO:0031032,GO:0031175,GO:0031248,GO:0031252,GO:0031344,GO:0031346,GO:0031365,GO:0031414,GO:0031416,GO:0032501,GO:0032502,GO:0032504,GO:0032506,GO:0032956,GO:0032970,GO:0032984,GO:0032989,GO:0032990,GO:0032991,GO:0033043,GO:0033267,GO:0036211,GO:0040011,GO:0042221,GO:0042330,GO:0042981,GO:0042995,GO:0043005,GO:0043066,GO:0043067,GO:0043069,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043523,GO:0043524,GO:0043543,GO:0043624,GO:0043933,GO:0044085,GO:0044087,GO:0044089,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044424,GO:0044444,GO:0044463,GO:0044464,GO:0044703,GO:0044837,GO:0044877,GO:0048308,GO:0048311,GO:0048468,GO:0048477,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0048609,GO:0048666,GO:0048667,GO:0048699,GO:0048731,GO:0048812,GO:0048856,GO:0048858,GO:0048869,GO:0048870,GO:0050776,GO:0050778,GO:0050789,GO:0050794,GO:0050896,GO:0051015,GO:0051128,GO:0051130,GO:0051179,GO:0051234,GO:0051261,GO:0051301,GO:0051493,GO:0051604,GO:0051640,GO:0051641,GO:0051646,GO:0051674,GO:0051704,GO:0060429,GO:0060491,GO:0060548,GO:0061024,GO:0061564,GO:0061640,GO:0065007,GO:0071704,GO:0071840,GO:0090130,GO:0090132,GO:0090723,GO:0097374,GO:0097435,GO:0097458,GO:0097485,GO:0098657,GO:0099024,GO:0120025,GO:0120032,GO:0120034,GO:0120035,GO:0120036,GO:0120038,GO:0120039,GO:0150034,GO:1901214,GO:1901215,GO:1901564,GO:1902407,GO:1902410,GO:1902493,GO:1902494,GO:1902743,GO:1902745,GO:1903047,GO:1903475,GO:1990234,GO:1990761
EggNOG free text desc.N-terminal peptidyl-methionine acetylation
EggNOG OGsKOG2053@1,KOG2053@2759
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03029
Hectar predicted targeting categoryother localisation
Exons2
Model size294
Cds size294
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927760.4117422-CDS-E-fasciculatus_F_contig12526:2643..28441622927760.4117422-CDS-E-fasciculatus_F_contig12526:2643..2844Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12526 2644..2844 -
1695995682.7665243-CDS-E-fasciculatus_F_contig12526:2643..28441695995682.7665243-CDS-E-fasciculatus_F_contig12526:2643..2844Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12526 2644..2844 -
1622927760.4257424-CDS-E-fasciculatus_F_contig12526:3381..34741622927760.4257424-CDS-E-fasciculatus_F_contig12526:3381..3474Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12526 3382..3474 -
1695995682.77764-CDS-E-fasciculatus_F_contig12526:3381..34741695995682.77764-CDS-E-fasciculatus_F_contig12526:3381..3474Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12526 3382..3474 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig12526.1942.1prot_E-fasciculatus_F_contig12526.1942.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig12526 2644..3474 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig12526.1942.1

>prot_E-fasciculatus_F_contig12526.1942.1 ID=prot_E-fasciculatus_F_contig12526.1942.1|Name=mRNA_E-fasciculatus_F_contig12526.1942.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=98bp
VQILAIDVYRQLGSFARALTIFGELDVKQIQMDTLSYLMLGPSLCHGLFG
EVQGQCKGIAAVHRSCKNDTAEHVATAFERGNYSKGAEVDRFQVCLV*
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mRNA from alignment at E-fasciculatus_F_contig12526:2644..3474-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig12526.1942.1 ID=mRNA_E-fasciculatus_F_contig12526.1942.1|Name=mRNA_E-fasciculatus_F_contig12526.1942.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=831bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12526:2644..3474- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GTGCAGATCCTGGCGATAGACGTGTACCGACAGCTTGGGTCGTTCGCGCG
GGCCCTGACGATCTTCGGAGAGCTGGACGTCAAGCAGATACAGGTATGTA
TGCAGGCCGCGGCACGCCGTGCTGAGTTTGTGTACTAGGCACGTGGTAGG
AAGATTTGTCTCGTGTGCGATTTGCTGTACGTCGATGTGGGAGAAGACGC
ACTCCCAATCTACACGCCACAATCTTGCTGTACTCGACGAAAACAAAACA
ACTACTCCGGACAGCTGCGTGTTTATTTTTTCTCGCCTTCCCGTCCGATC
GTCTTGTTTCCGAGAGAGCCTGACACCCCGCATTGAATCTACTTGAATAT
TCCACTTGCCACGGGTTTTCGTCCGCCTTTCGCAGGGTACTCGGCCTGCT
GTACGCGGACCACTACTGCTACCACTAATGCGTGTGACCCCCCCCCATCC
ATGTGTTCAAATCTCTTCACAGACGGCCTTAGTACAAGGACCTCTGCCTC
CCCTTTTTACCCGCCGCGCCCGCGAAGAGCCGGCAAATCGCTCCTATGCG
AGCTCAATCGTCTAACGACTACGCCATGCGTTTTGCACGAGTACACCCGC
CCTAATCTTCGAATATCCCCCCCCCCCCAGATGGATACCCTGTCGTACCT
AATGCTGGGGCCGAGCCTCTGCCACGGCCTGTTCGGAGAAGTGCAGGGCC
AGTGCAAGGGCATCGCTGCGGTGCACCGGAGTTGCAAGAACGACACGGCC
GAGCACGTGGCGACCGCGTTTGAGCGGGGGAACTATTCCAAGGGGGCCGA
GGTCGATCGCTTCCAGGTGTGTTTGGTGTGA
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig12526:2644..3474-

>mRNA_E-fasciculatus_F_contig12526.1942.1 ID=mRNA_E-fasciculatus_F_contig12526.1942.1|Name=mRNA_E-fasciculatus_F_contig12526.1942.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=588bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12526:2644..3474- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GTGCAGATCCTGGCGATAGACGTGTACCGACAGCTTGGGTCGTTCGCGCG
GGCCCTGACGATCTTCGGAGAGCTGGACGTCAAGCAGATACAGGTGCAGA
TCCTGGCGATAGACGTGTACCGACAGCTTGGGTCGTTCGCGCGGGCCCTG
ACGATCTTCGGAGAGCTGGACGTCAAGCAGATACAGATGGATACCCTGTC
GTACCTAATGCTGGGGCCGAGCCTCTGCCACGGCCTGTTCGGAGAAGTGC
AGGGCCAGTGCAAGGGCATCGCTGCGGTGCACCGGAGTTGCAAGAACGAC
ACGGCCGAGCACGTGGCGACCGCGTTTGAGCGGGGGAACTATTCCAAGGG
GGCCGAGGTCGATCGCTTCCAGGTGTGTTTGGTGTGAATGGATACCCTGT
CGTACCTAATGCTGGGGCCGAGCCTCTGCCACGGCCTGTTCGGAGAAGTG
CAGGGCCAGTGCAAGGGCATCGCTGCGGTGCACCGGAGTTGCAAGAACGA
CACGGCCGAGCACGTGGCGACCGCGTTTGAGCGGGGGAACTATTCCAAGG
GGGCCGAGGTCGATCGCTTCCAGGTGTGTTTGGTGTGA
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