mRNA_E-fasciculatus_F_contig1250.1924.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig1250.1924.1
Unique NamemRNA_E-fasciculatus_F_contig1250.1924.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig1250.1924.1 vs. uniprot
Match: D7FXR5_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXR5_ECTSI)

HSP 1 Score: 81.3 bits (199), Expect = 2.470e-14
Identity = 49/84 (58.33%), Postives = 56/84 (66.67%), Query Frame = 1 
Query:   13 EVSALIGVVTVMAMLHVFSSPARNDTGTVADSFKEPYAATSVGSTKRRCIRLFGRVVFVLVMFGTYVGLESVIRWTSPDSKVKP 264
            +V+AL+GV+T MA L VFSSPAR DTG VA SFKE        S K R IRLF RV FV V+F T+ GLESVIR +S     KP
Sbjct:    5 KVAALVGVMTAMAGLLVFSSPARCDTGIVAASFKELDDGEKSRSAKHRWIRLFVRVFFVYVVFATFAGLESVIRRSSMVFTAKP 88
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig1250.1924.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 1
Match NameE-valueIdentityDescription
D7FXR5_ECTSI2.470e-1458.33Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig1250contigE-fasciculatus_F_contig1250:9171..14687 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Hectar predicted targeting categorysignal peptide
Exons3
Model size1605
Cds size966
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig1250.1924.1prot_E-fasciculatus_F_contig1250.1924.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig1250 9171..14048 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927756.712252-CDS-E-fasciculatus_F_contig1250:9170..92841622927756.712252-CDS-E-fasciculatus_F_contig1250:9170..9284Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1250 9171..9284 +
1695995681.6952376-CDS-E-fasciculatus_F_contig1250:9170..92841695995681.6952376-CDS-E-fasciculatus_F_contig1250:9170..9284Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1250 9171..9284 +
1622927756.7241552-CDS-E-fasciculatus_F_contig1250:12851..129981622927756.7241552-CDS-E-fasciculatus_F_contig1250:12851..12998Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1250 12852..12998 +
1695995681.7051668-CDS-E-fasciculatus_F_contig1250:12851..129981695995681.7051668-CDS-E-fasciculatus_F_contig1250:12851..12998Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1250 12852..12998 +
1622927756.7361946-CDS-E-fasciculatus_F_contig1250:13343..140481622927756.7361946-CDS-E-fasciculatus_F_contig1250:13343..14048Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1250 13344..14048 +
1695995681.7135804-CDS-E-fasciculatus_F_contig1250:13343..140481695995681.7135804-CDS-E-fasciculatus_F_contig1250:13343..14048Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1250 13344..14048 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927756.7493722-UTR-E-fasciculatus_F_contig1250:14048..146871622927756.7493722-UTR-E-fasciculatus_F_contig1250:14048..14687Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig1250 14049..14687 +
1695995681.7222502-UTR-E-fasciculatus_F_contig1250:14048..146871695995681.7222502-UTR-E-fasciculatus_F_contig1250:14048..14687Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig1250 14049..14687 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig1250.1924.1

>prot_E-fasciculatus_F_contig1250.1924.1 ID=prot_E-fasciculatus_F_contig1250.1924.1|Name=mRNA_E-fasciculatus_F_contig1250.1924.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=322bp
MPGSEVSALIGVVTVMAMLHVFSSPARNDTGTVADSFKEPYAATSVGSTK
RRCIRLFGRVVFVLVMFGTYVGLESVIRWTSPDSKVKPPVEEGGHNDVLD
ERGEKEKREAAAEEARRRIEKEKKEAAAEEARRRVENEKREAAAAEERRR
IEKEKREAAAEEERRRIEKEKKEAAAEEARRRVEKEKREAAAEEERRRVE
KEKREAAAEEERRRIENEKKEAAAEEAKRRKQRENYDTYRKRAEEKQRVK
IEKEQRESPEEMRRRKVDEYYESRKRSAADEGTPRIEKEERESPEEMRRR
KMGEYYEARKRNAAGKASAKA*
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mRNA from alignment at E-fasciculatus_F_contig1250:9171..14687+

Legend: polypeptideCDSUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig1250.1924.1 ID=mRNA_E-fasciculatus_F_contig1250.1924.1|Name=mRNA_E-fasciculatus_F_contig1250.1924.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=5517bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1250:9171..14687+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
ATGCCCGGGAGTGAAGTCTCTGCACTTATCGGCGTTGTGACGGTCATGGC
AATGCTTCATGTATTCTCGTCACCAGCTCGGAATGACACAGGAACAGTCG
CAGATAGTTTCAAGGTGAGAGTCAGCCTCATCTCCGCTTCCTATGGGGAT
CTCCATTTGACGGCACAAGCCCGTTTACGTCGGTTCGTGCACTACAGCGT
AAGGTCCTCACACCGTTTGTCGACCAATAACCGCTACACTTCTGCATCCA
CACAAACCACATGGACTACATAACTAGACTGCGTAGACAAAGTATACTCT
TGTTATCTGTAGTGCACTACAGCCGTACATCGGGGAACGAGATATCCTCT
TGGTTGCATTTCTCGGTCTAAACTGTTGTATTCTCGTATGGTATCCGTTT
TTGGATCACTGATAGAAAATATTGGACTTGTTGTTCGGATGACTCTTCCT
GTCCCGTCGCAAGAACTTACGTACCGAAACTTCGTTCCGTATCCTCCGCC
ATCGTATAACGGCCAGATGTTATACCTCGATCCTAAACACGCCGAACCCC
AAACTCTCTATTCGGTGCTTTTTCCACCACCACACGCGCTAAGCGCTTCC
GTGAAGCCCTTACTTAACCACATACGATCTGCCTGGTCATATCAATATCC
AACTTTTTCCGTTTCCCTTGTTTGGAGTCGCCATCCAACTTTTGCCGGTT
CCCTTTTGGTGGGGTCGCTATTTAGTCGTCTGCCCAATGCAGTCCCTTGC
CACCGACTTGGTTTTCCTGCCACCGATCTTGAAACTTGGTCTACATGTCC
GCGGTACCTGCCAAGGATTCGCTTAGCGAACCGCCGACCCGAAACAGGTG
CATCGTAAAGTGGGTAGGAAGCTTCGCTACCCGAAGGTGTGCCTGTTAAT
TCCGCGGTCATGCGAGAGGCAGTCAGTGGCAGAGCGCCCGCCCCGACTGC
AGTCCGCGGCCACCGCCGGGAACAGATGAACCGGCTGCCAAACCTAGATG
TTTCGCCGCTGCAGGATAAGCCTGCACAGCGCGCAACCGCACACGTGCCA
AGGTTGTCACTGTCCGCCAGCACGACCCCAAGGATTCCGTCGACACCCGC
AGCGTTTTCCCGAAGATACAATTCAAGATTAACCCCCCTTTCATCCGGCA
AGCGCAAAAACTTTGATCCCATCGTAGACGACAAACCGAAACGCCTGCGC
ATTGTGTAGAACGACAGAGCAGACAGCGTGATGTCTCGTACATTCAATGG
ATATATGTTCACCGAGGATGAGAGTTGCGCCCGGATCCGCATATTCTCCA
GCGAGAGCTCCAGCGTGTGGGCTAACAAAGGCGCAGTCTGCTTTGCCGGG
ACCCCACTCTGCTTTTGCTCTTCTGATACGAATGTCATATACGACTCCAC
CAGGGGTGAGTTCGCACGGGTTTCCGATCCTGTCGAGAGACTTCATGGGT
CGTGTTTTCCCAACTGCTCCCAACACGCCGTCCGTAGCGCGGAAAAAAAA
CGTTTTCGAAGCGATTAGGCCGCGTACCGCTTCGCACACGGGGACGTTGG
GGAACACCTATGCCCCCCCATTGAGCCTGTGATTCTAGAAAACACAACCA
GTCAAAGACATCGTCCGCGGTGGCCGATTCCCTTTTGGCCAGCCGAGTTC
GAGCGGGTAAATCTGTCGAAATTGGCAGCGGCGTTTCCTTTTTCGGACTT
GTCCAGCCCGTCCCGACACAGCTTCACGCACTTGCCGGCGCGGGTCTCGC
AGCGGGTGGCGACTGATGGCGATAGGCTGAGTCAACCGCGGTGGGGGCCA
GTTGGCAAGTCGGGCGCTCGCATTTCCAAAAGAAGTTGAATTGACCAGCG
TCCTCTGTGCGACACTCGGCACAACACCGCCAACCGAACAAGTTCCCGGG
CACTCGCCGGACCCGCTGCCCCCCTCCGTGTAGACGAGAACGCCAGGAAT
GAAAAACATTCGGAAAACAAAAGGACACAATCCCTATCATCGGGCGTTCA
TCTGAACAGTTGGCAACGTGGATACTAGACACTCCCCCGGGTAGTAAGAA
TCGAAATCTGTTTCGTGTGAGCTACCATAGCCCATCGGGAGTGAGTAGCG
CCATGGAGGTAGTTAAGCCTTTGGGAATCGGCCACTGGAAACACCATTGG
CCCAACCCGTGGGGCGACTTCGAGCGACCGACGCGTCGCAAACTGCAGCA
ACGGAAACCAGTGTTCGCGAACATCGGGGAGGAGAAGGACTGCACGCTCG
CGGCACTCCGCCAAAATGTTGGACGATGTGCCCAGCCGTCAAGGCCGGCG
GGAAACAGAAACCAAACGCTGGACCTCCGCCCGCCGGCAGCACCGCGACG
TTCTGCGCCAGCGCGTCCGTGCCTTACGAGCCGGCGAAAGTGCACCTAGA
AAAGAACTGCAGGGGGGGGCCCGAAGGTGGACACCAGTAGGCCGACGCCG
TACACGCCATGAGGTCGATGTTCAGCGGTCCAAAATCCTCCCACACAACC
CGGAAAGCCACCGGCCTGAGGCGCGTGATAGTACCCCGGGGCGGCCGCGA
GGTGGCGTCGGCTAGCCCATTAGCCGCTATCGGTATCCATTGCAATGGGA
GCACGAAGTCGTAGTTCAATTTCAGCTCGAAGAGTTGAATGAGGAGATCG
TGCGTAAGGTGGTGATTGGCGCGCCCCCGCTTAAACGCACCGACAACGAA
TTCGTTGTCCACATTGATGACGATCTGTGTACGCTGCAACGCAACGCCCT
GCCTGACACATTTCTCTGTATTTACGACGCTATTGGGGAAAAAACTGGAA
ACGATCTATAGTCTCGTTCATGTCTAAACGTTTGGGGGGGGTGGACTACG
TGCCGAATCCGTTCCCCTTTTGAGACCTCCCCGATATGAAGTATGCCGTT
TTCACGGGTCGTTGGCGGTCTAATCTCGATCTAACATTCGCACTGTTATT
TCCCGAGCATCGACTTGAAAACACATTCCGAGTTCGACAGACAATTTCTT
TCCCGGATCTGAACCCTGATTGGCGAACTCTTCTGCTTTAAACACAAGCC
TAAGACAATGAAACAAAACTCCAAACACCTGACCATACCATGGAATAACA
GCGAAGCGTTGCGTACCACGGCACGGTATTTCCCGATGCCGCTACCTGCG
GTTCAACTGTCATCGCCGTAGAATGGATGTCCAGCCACGCTTGGCTTCAA
CGGGGAAGGAGAACCAGTTGTTGTGGGGGGAGGGTATTCGTGGCTGATGG
TTTGCCGGGGGGGGCGTGGTTTGATTGACAACAACCTCGGGCAGGCATTA
TCCTGTCGCAAGGGTAAGTGGCTGTCGATGATCCCGTGGATTTATCAATG
TTAACAGAAAAGGCCGGGAACATACATCCGTTCCACACCGTCTCGTTCAA
CTTCGACGTCCGTCTGCACGAAGGCATTAGCAAAACACAATATTCTCTAT
AGTGTCTGTATGCGACCCCAACACGCTCATCACGATTCAACATGGATAGA
CGTGAAGTGAGAAACAACAACGCACGGTAGTTAATTGCAAGTGAGAACTG
GAATGAAGAACCTCTGCAGTTCCAAAGGCTGCCCGAAAGGAAACCTGACA
CGTGCCCAACCGTTATCTGCTTGGCCGCGTGAACCGAAAACATATGGTGG
TGGAATTGATGGGCGCGCGGCTCGCAAAAAGGAACCCTATGCCGCAACAA
GCGTCGGCTCCACGAAGCGCAGATGCATCCGACTCTTTGGGCGGGTGGTG
TTCGTGTTGGTTATGTTCGGGACGTACGTCGGACTCGAGTCTGTGATTCG
CTGGACATCACCGGATTCCAAGGTCAAGGTAAGGGCGTGATACGTGTTCG
TTCAGCACTGTGGACGAGATCAAGCTACGCATAGTCTTCCCAGAGCTGGG
GGCTAGTCTTCCCAGAGTTAGGGGCCCAATCTGGCGCACTCTTCATGATT
TTCGTTTACATCGAAGCTAATGTGTGGTGTTTCGTACGGGGGGCATACAC
CATTTCTTTGGCTTGCGGCCACGCTGCCAAAGACGAACATGTCCGTCTCA
AGTTCAATTTTTTCCTCTTGATAATTCTTCGCCACCTGCTTGCTATACTT
TGTTGTGTCGACGTCGTCGCTTTCGTCCCCCGGATTTCCTGACGTTCTGA
TCTTATTCCGAATATGGACGTAGCCGCCGGTAGAAGAGGGCGGGCATAAT
GACGTACTCGACGAAAGAGGCGAGAAAGAGAAGAGGGAAGCTGCCGCTGA
AGAAGCGAGGCGCAGGATCGAGAAAGAGAAGAAGGAAGCTGCCGCTGAAG
AAGCGAGGCGCAGGGTCGAGAACGAGAAGAGGGAAGCTGCCGCTGCGGAA
GAGAGGCGCAGGATCGAGAAAGAGAAGAGGGAAGCTGCCGCTGAAGAAGA
GAGGCGCAGGATCGAGAAAGAGAAGAAGGAAGCTGCCGCTGAAGAAGCGA
GGCGCAGGGTCGAGAAAGAGAAGAGGGAAGCTGCCGCTGAAGAAGAGAGG
CGCAGGGTCGAGAAAGAGAAGAGGGAAGCTGCCGCTGAAGAAGAGAGGCG
CAGGATCGAGAATGAGAAGAAGGAAGCTGCCGCTGAAGAAGCGAAGCGCA
GAAAACAGCGGGAAAATTACGACACATACAGGAAGAGAGCCGAGGAAAAA
CAGAGGGTTAAGATCGAGAAAGAGCAGAGGGAGAGCCCTGAAGAAATGAG
ACGCAGAAAGGTAGACGAATACTACGAATCACGAAAGAGGAGCGCTGCGG
ATGAAGGGACGCCCAGGATCGAGAAAGAGGAGAGGGAGAGCCCTGAAGAA
ATGCGACGCAGAAAGATGGGCGAGTATTACGAAGCACGAAAGAGAAATGC
TGCGGGAAAAGCGAGTGCGAAAGCGTAGAAGGAGGCCGCTGAAAAAGAGA
GTGCCAGCAGTGAGGGCGAATAATTCGAGGCACAAAGAAGGAGAGCTGAG
GCAAGAGGGAGACGTAGGAGTGCGAATGAGTTTCAGAGGGACACGAACAT
TTCAGGGGCGAGATATGTGTTGGAACCTCTGCGAGACATGGCTAAGACTA
GCAGCGAGGACAAAGGCCTTAGTGATTTTTCCGGTTCAGTTCGCCAGGCA
GGCCAAATTAATAGCCGTGTTCTGTTCTTGGCAGCCCCACCCGTCCTCGA
TGCGATTGCATGCTGGGTGTTTCATGTATGAAACCTACGGAAGCGAACGG
AATACTCAACCGGGGAGAGGAGGGTCGTAATAGTTCTACCCGCGTGGTGT
TTCGAACAGGGTATAATGTAGCGGCCGTAGAGTACTTGCATAGGATGGGC
AAATAATATGGGCGTCGTCACAGTTTTTGTCTCCGTTGATCCGATGTGCT
CTTTTTGGGTGCTTCACCATGTTCCATGTCAGGGCGGGCTTGCTTGTGAA
ACAGGATCTGTGTGCCTGTCGCGTGAAGAATGTGACCACATCTACTACGT
AGAGAGCGTGGGGCGCGCGAGGGGGTGCGGGATATGTGCCGGACGAGATA
TTGGCACAAGAATCGTG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig1250:9171..14687+

>mRNA_E-fasciculatus_F_contig1250.1924.1 ID=mRNA_E-fasciculatus_F_contig1250.1924.1|Name=mRNA_E-fasciculatus_F_contig1250.1924.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=1932bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1250:9171..14687+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
ATGCCCGGGAGTGAAGTCTCTGCACTTATCGGCGTTGTGACGGTCATGGC
AATGCTTCATGTATTCTCGTCACCAGCTCGGAATGACACAGGAACAGTCG
CAGATAGTTTCAAGATGCCCGGGAGTGAAGTCTCTGCACTTATCGGCGTT
GTGACGGTCATGGCAATGCTTCATGTATTCTCGTCACCAGCTCGGAATGA
CACAGGAACAGTCGCAGATAGTTTCAAGGAACCCTATGCCGCAACAAGCG
TCGGCTCCACGAAGCGCAGATGCATCCGACTCTTTGGGCGGGTGGTGTTC
GTGTTGGTTATGTTCGGGACGTACGTCGGACTCGAGTCTGTGATTCGCTG
GACATCACCGGATTCCAAGGTCAAGGAACCCTATGCCGCAACAAGCGTCG
GCTCCACGAAGCGCAGATGCATCCGACTCTTTGGGCGGGTGGTGTTCGTG
TTGGTTATGTTCGGGACGTACGTCGGACTCGAGTCTGTGATTCGCTGGAC
ATCACCGGATTCCAAGGTCAAGCCGCCGGTAGAAGAGGGCGGGCATAATG
ACGTACTCGACGAAAGAGGCGAGAAAGAGAAGAGGGAAGCTGCCGCTGAA
GAAGCGAGGCGCAGGATCGAGAAAGAGAAGAAGGAAGCTGCCGCTGAAGA
AGCGAGGCGCAGGGTCGAGAACGAGAAGAGGGAAGCTGCCGCTGCGGAAG
AGAGGCGCAGGATCGAGAAAGAGAAGAGGGAAGCTGCCGCTGAAGAAGAG
AGGCGCAGGATCGAGAAAGAGAAGAAGGAAGCTGCCGCTGAAGAAGCGAG
GCGCAGGGTCGAGAAAGAGAAGAGGGAAGCTGCCGCTGAAGAAGAGAGGC
GCAGGGTCGAGAAAGAGAAGAGGGAAGCTGCCGCTGAAGAAGAGAGGCGC
AGGATCGAGAATGAGAAGAAGGAAGCTGCCGCTGAAGAAGCGAAGCGCAG
AAAACAGCGGGAAAATTACGACACATACAGGAAGAGAGCCGAGGAAAAAC
AGAGGGTTAAGATCGAGAAAGAGCAGAGGGAGAGCCCTGAAGAAATGAGA
CGCAGAAAGGTAGACGAATACTACGAATCACGAAAGAGGAGCGCTGCGGA
TGAAGGGACGCCCAGGATCGAGAAAGAGGAGAGGGAGAGCCCTGAAGAAA
TGCGACGCAGAAAGATGGGCGAGTATTACGAAGCACGAAAGAGAAATGCT
GCGGGAAAAGCGAGTGCGAAAGCGTAGCCGCCGGTAGAAGAGGGCGGGCA
TAATGACGTACTCGACGAAAGAGGCGAGAAAGAGAAGAGGGAAGCTGCCG
CTGAAGAAGCGAGGCGCAGGATCGAGAAAGAGAAGAAGGAAGCTGCCGCT
GAAGAAGCGAGGCGCAGGGTCGAGAACGAGAAGAGGGAAGCTGCCGCTGC
GGAAGAGAGGCGCAGGATCGAGAAAGAGAAGAGGGAAGCTGCCGCTGAAG
AAGAGAGGCGCAGGATCGAGAAAGAGAAGAAGGAAGCTGCCGCTGAAGAA
GCGAGGCGCAGGGTCGAGAAAGAGAAGAGGGAAGCTGCCGCTGAAGAAGA
GAGGCGCAGGGTCGAGAAAGAGAAGAGGGAAGCTGCCGCTGAAGAAGAGA
GGCGCAGGATCGAGAATGAGAAGAAGGAAGCTGCCGCTGAAGAAGCGAAG
CGCAGAAAACAGCGGGAAAATTACGACACATACAGGAAGAGAGCCGAGGA
AAAACAGAGGGTTAAGATCGAGAAAGAGCAGAGGGAGAGCCCTGAAGAAA
TGAGACGCAGAAAGGTAGACGAATACTACGAATCACGAAAGAGGAGCGCT
GCGGATGAAGGGACGCCCAGGATCGAGAAAGAGGAGAGGGAGAGCCCTGA
AGAAATGCGACGCAGAAAGATGGGCGAGTATTACGAAGCACGAAAGAGAA
ATGCTGCGGGAAAAGCGAGTGCGAAAGCGTAG
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