mRNA_E-fasciculatus_F_contig12271.1754.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig12271.1754.1
Unique NamemRNA_E-fasciculatus_F_contig12271.1754.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig12271.1754.1 vs. uniprot
Match: A0A6H5KZD5_9PHAE (RF_PROK_I domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KZD5_9PHAE)

HSP 1 Score: 148 bits (374), Expect = 3.180e-41
Identity = 73/74 (98.65%), Postives = 74/74 (100.00%), Query Frame = 1 
Query:    4 VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILLNQQELMAELAAKQRQGQEGGVASR 225
            VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILL+QQELMAELAAKQRQGQEGGVASR
Sbjct:  375 VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILLDQQELMAELAAKQRQGQEGGVASR 448
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BLAST of mRNA_E-fasciculatus_F_contig12271.1754.1 vs. uniprot
Match: A0A836CKN4_9STRA (Peptide chain release factor 1 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CKN4_9STRA)

HSP 1 Score: 99.4 bits (246), Expect = 4.190e-23
Identity = 47/66 (71.21%), Postives = 58/66 (87.88%), Query Frame = 1 
Query:    4 VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILLNQQELMAELAAKQRQG 201
            VGSG+RSEKIRTYNWKDSRCSDHRI +N+PL+ FLSG+ +DDMI ACI+++QQE + E+A KQ QG
Sbjct:  303 VGSGARSEKIRTYNWKDSRCSDHRINRNWPLDTFLSGT-IDDMIAACIVMDQQEQLQEMARKQAQG 367
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BLAST of mRNA_E-fasciculatus_F_contig12271.1754.1 vs. uniprot
Match: A0A6U2GK04_HEMAN (Hypothetical protein n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A6U2GK04_HEMAN)

HSP 1 Score: 90.9 bits (224), Expect = 8.870e-20
Identity = 42/59 (71.19%), Postives = 51/59 (86.44%), Query Frame = 1 
Query:    4 VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILLNQQELMAEL 180
            VG+GSRSEKIRTYNWKD RCSDHR+GKNFPLN+F++GS +D MI  CI  +QQE +A+L
Sbjct:  366 VGTGSRSEKIRTYNWKDGRCSDHRLGKNFPLNDFMAGS-IDGMISECIFKDQQEKLAQL 423
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BLAST of mRNA_E-fasciculatus_F_contig12271.1754.1 vs. uniprot
Match: A0A7S0E3D2_9CRYP (Hypothetical protein n=1 Tax=Hanusia phi TaxID=3032 RepID=A0A7S0E3D2_9CRYP)

HSP 1 Score: 83.2 bits (204), Expect = 1.120e-18
Identity = 39/60 (65.00%), Postives = 48/60 (80.00%), Query Frame = 1 
Query:    4 VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILLNQQELMAELA 183
            VG+GSRSEKIRTYNWKD RCSDHR+ KNFPL  FLSG  L+ +I  CI  +QQ+ +A++A
Sbjct:   78 VGTGSRSEKIRTYNWKDGRCSDHRLNKNFPLQQFLSGE-LESIIQECIFKDQQQKLADMA 136
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BLAST of mRNA_E-fasciculatus_F_contig12271.1754.1 vs. uniprot
Match: A0A7S3JVW2_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3JVW2_9STRA)

HSP 1 Score: 84.7 bits (208), Expect = 1.100e-17
Identity = 40/62 (64.52%), Postives = 50/62 (80.65%), Query Frame = 1 
Query:    4 VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILLNQQELMAELAAK 189
            VG+G RSEKIRTYNWKDSRC+DHR+ +NFPL+  L+G  LD +I ACI L Q E+++ELA K
Sbjct:  304 VGTGGRSEKIRTYNWKDSRCTDHRLNQNFPLDKVLNGD-LDPIISACIALAQSEMLSELAEK 364
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BLAST of mRNA_E-fasciculatus_F_contig12271.1754.1 vs. uniprot
Match: A0A6S9M8F3_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6S9M8F3_HETAK)

HSP 1 Score: 84.7 bits (208), Expect = 1.450e-17
Identity = 41/63 (65.08%), Postives = 52/63 (82.54%), Query Frame = 1 
Query:    4 VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILLNQQELMAELAAKQ 192
            VG+G+RSEKIRTYNWKDSRCSDHRIG+NFPL  FL G  +DD+I A I+ +QQ+ + E+A +Q
Sbjct:  351 VGTGARSEKIRTYNWKDSRCSDHRIGQNFPLQMFLDGK-VDDIIQALIVSDQQDQLKEMAEEQ 412
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BLAST of mRNA_E-fasciculatus_F_contig12271.1754.1 vs. uniprot
Match: A0A140ECJ7_9STRA (Plastid peptide chain release factor 1 n=1 Tax=Trachydiscus minutus TaxID=1032745 RepID=A0A140ECJ7_9STRA)

HSP 1 Score: 84.7 bits (208), Expect = 1.510e-17
Identity = 38/65 (58.46%), Postives = 52/65 (80.00%), Query Frame = 1 
Query:    4 VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILLNQQELMAELAAKQRQ 198
            +GSG RSEKIRTYNWKDSRC+DHR+G+NF L+ FLSG  +D+MI  C+L+ Q E + +++ +Q Q
Sbjct:  362 IGSGGRSEKIRTYNWKDSRCTDHRLGQNFNLDKFLSGE-IDEMINQCLLMEQNEKLEQMSKEQLQ 425
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BLAST of mRNA_E-fasciculatus_F_contig12271.1754.1 vs. uniprot
Match: A0A1Z5KKR0_FISSO (Peptide chain release factor 1 n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5KKR0_FISSO)

HSP 1 Score: 84.3 bits (207), Expect = 1.840e-17
Identity = 41/59 (69.49%), Postives = 48/59 (81.36%), Query Frame = 1 
Query:    4 VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILLNQQELMAEL 180
            VG+GSRSEKIRTYNWKDSRCSDHR+G+NFPL  FLSG  +D MI A I  +Q+E + EL
Sbjct:  335 VGTGSRSEKIRTYNWKDSRCSDHRLGQNFPLQQFLSGD-IDGMISAMIAKDQEEKLREL 392
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BLAST of mRNA_E-fasciculatus_F_contig12271.1754.1 vs. uniprot
Match: A0A448Z911_9STRA (RF_PROK_I domain-containing protein n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448Z911_9STRA)

HSP 1 Score: 83.6 bits (205), Expect = 3.630e-17
Identity = 40/60 (66.67%), Postives = 49/60 (81.67%), Query Frame = 1 
Query:    4 VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILLNQQELMAELA 183
            VG+G RSEKIRTYNWKDSRCSDHRI +N+PLN FL G  +D +IGA I+ +Q+E + ELA
Sbjct:  344 VGTGGRSEKIRTYNWKDSRCSDHRIQQNYPLNQFLGGD-IDPIIGAMIMKDQEEKLKELA 402
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BLAST of mRNA_E-fasciculatus_F_contig12271.1754.1 vs. uniprot
Match: L1JFM6_GUITC (RF_PROK_I domain-containing protein n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1JFM6_GUITC)

HSP 1 Score: 83.2 bits (204), Expect = 3.800e-17
Identity = 39/60 (65.00%), Postives = 48/60 (80.00%), Query Frame = 1 
Query:    4 VGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACILLNQQELMAELA 183
            VG+GSRSEKIRTYNWKD RCSDHR+ KNFPL  FLSG  L+ +I  CI  +QQ+ +A++A
Sbjct:  296 VGTGSRSEKIRTYNWKDGRCSDHRLNKNFPLQQFLSGD-LESIIQECIFKDQQQKLADMA 354
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig12271.1754.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KZD5_9PHAE3.180e-4198.65RF_PROK_I domain-containing protein n=2 Tax=Ectoca... [more]
A0A836CKN4_9STRA4.190e-2371.21Peptide chain release factor 1 n=1 Tax=Tribonema m... [more]
A0A6U2GK04_HEMAN8.870e-2071.19Hypothetical protein n=1 Tax=Hemiselmis andersenii... [more]
A0A7S0E3D2_9CRYP1.120e-1865.00Hypothetical protein n=1 Tax=Hanusia phi TaxID=303... [more]
A0A7S3JVW2_9STRA1.100e-1764.52Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A6S9M8F3_HETAK1.450e-1765.08Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A140ECJ7_9STRA1.510e-1758.46Plastid peptide chain release factor 1 n=1 Tax=Tra... [more]
A0A1Z5KKR0_FISSO1.840e-1769.49Peptide chain release factor 1 n=2 Tax=Fistulifera... [more]
A0A448Z911_9STRA3.630e-1766.67RF_PROK_I domain-containing protein n=1 Tax=Pseudo... [more]
L1JFM6_GUITC3.800e-1765.00RF_PROK_I domain-containing protein n=1 Tax=Guilla... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig12271contigE-fasciculatus_F_contig12271:18..245 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score151.8
Seed ortholog evalue1.3e-34
Seed eggNOG ortholog2880.D7FY16
Preferred nameMRF1
KEGG koko:K02835
GOsGO:0003674,GO:0003676,GO:0003723,GO:0003747,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005829,GO:0006412,GO:0006415,GO:0006518,GO:0006807,GO:0006996,GO:0008079,GO:0008135,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009507,GO:0009536,GO:0009657,GO:0009658,GO:0009668,GO:0009987,GO:0010027,GO:0010467,GO:0016020,GO:0016043,GO:0016149,GO:0019538,GO:0019866,GO:0022411,GO:0031090,GO:0031966,GO:0031967,GO:0031975,GO:0032543,GO:0032544,GO:0032984,GO:0034641,GO:0034645,GO:0043043,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043603,GO:0043604,GO:0043624,GO:0043933,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0061024,GO:0070126,GO:0071704,GO:0071840,GO:0097159,GO:0140053,GO:1901363,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.translation release factor activity
EggNOG OGsCOG0216@1,KOG2726@2759
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03012
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionpeptide chain release factor 1
Ec32 orthologEc-08_004700.1
Exons1
Model size228
Cds size228
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927734.4502573-CDS-E-fasciculatus_F_contig12271:17..2451622927734.4502573-CDS-E-fasciculatus_F_contig12271:17..245Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12271 18..245 +
1695995671.6548939-CDS-E-fasciculatus_F_contig12271:17..2451695995671.6548939-CDS-E-fasciculatus_F_contig12271:17..245Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12271 18..245 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig12271.1754.1prot_E-fasciculatus_F_contig12271.1754.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig12271 18..245 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig12271.1754.1

>prot_E-fasciculatus_F_contig12271.1754.1 ID=prot_E-fasciculatus_F_contig12271.1754.1|Name=mRNA_E-fasciculatus_F_contig12271.1754.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=76bp
KVGSGSRSEKIRTYNWKDSRCSDHRIGKNFPLNNFLSGSGLDDMIGACIL
LNQQELMAELAAKQRQGQEGGVASR*
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mRNA from alignment at E-fasciculatus_F_contig12271:18..245+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig12271.1754.1 ID=mRNA_E-fasciculatus_F_contig12271.1754.1|Name=mRNA_E-fasciculatus_F_contig12271.1754.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=228bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12271:18..245+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
AAGGTGGGATCTGGATCGCGCTCGGAGAAGATCAGGACGTACAACTGGAA
GGACAGCCGGTGCTCCGACCACCGAATAGGGAAGAATTTCCCGCTCAACA
ACTTCCTGTCGGGCAGCGGCCTGGACGACATGATCGGGGCCTGCATCCTC
CTGAACCAGCAGGAGCTCATGGCCGAGCTCGCGGCGAAGCAGCGGCAGGG
CCAGGAAGGCGGCGTAGCCAGCAGATAG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig12271:18..245+

>mRNA_E-fasciculatus_F_contig12271.1754.1 ID=mRNA_E-fasciculatus_F_contig12271.1754.1|Name=mRNA_E-fasciculatus_F_contig12271.1754.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=456bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12271:18..245+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
AAGGTGGGATCTGGATCGCGCTCGGAGAAGATCAGGACGTACAACTGGAA
GGACAGCCGGTGCTCCGACCACCGAATAGGGAAGAATTTCCCGCTCAACA
ACTTCCTGTCGGGCAGCGGCCTGGACGACATGATCGGGGCCTGCATCCTC
CTGAACCAGCAGGAGCTCATGGCCGAGCTCGCGGCGAAGCAGCGGCAGGG
CCAGGAAGGCGGCGTAGCCAGCAGATAGAAGGTGGGATCTGGATCGCGCT
CGGAGAAGATCAGGACGTACAACTGGAAGGACAGCCGGTGCTCCGACCAC
CGAATAGGGAAGAATTTCCCGCTCAACAACTTCCTGTCGGGCAGCGGCCT
GGACGACATGATCGGGGCCTGCATCCTCCTGAACCAGCAGGAGCTCATGG
CCGAGCTCGCGGCGAAGCAGCGGCAGGGCCAGGAAGGCGGCGTAGCCAGC
AGATAG
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