mRNA_E-fasciculatus_F_contig12131.1663.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig12131.1663.1
Unique NamemRNA_E-fasciculatus_F_contig12131.1663.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig12131.1663.1 vs. uniprot
Match: D7FT60_ECTSI (Nitroreductase-like protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FT60_ECTSI)

HSP 1 Score: 130 bits (328), Expect = 7.000e-33
Identity = 66/72 (91.67%), Postives = 68/72 (94.44%), Query Frame = 1 
Query:  520 LAAADSDAEAFERVTLARRSTQHFDRRRPVPDDVLKKVLALVLGTPSGFNAQPYECIVVTSDEAKEKLSKAM 735
            LAAADSDAEAFERVTLARRST+HFDRRRPVPDDVLKKVLALVL  PSGFN QPYECIVVTSDEAK KLS+AM
Sbjct:   65 LAAADSDAEAFERVTLARRSTKHFDRRRPVPDDVLKKVLALVLRAPSGFNIQPYECIVVTSDEAKHKLSRAM 136
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BLAST of mRNA_E-fasciculatus_F_contig12131.1663.1 vs. uniprot
Match: K3WSR0_GLOUD (Nitroreductase domain-containing protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WSR0_GLOUD)

HSP 1 Score: 57.4 bits (137), Expect = 4.400e-6
Identity = 28/65 (43.08%), Postives = 37/65 (56.92%), Query Frame = 1 
Query:  541 AEAFERVTLARRSTQHFDRRRPVPDDVLKKVLALVLGTPSGFNAQPYECIVVTSDEAKEKLSKAM 735
            A+   R  ++ R+T  F   +PVPD  L +VL L    PSGFN QPY C+VV     +EK + AM
Sbjct:   57 AQKLLRSAISDRATVRFFSDKPVPDATLAEVLRLTQRAPSGFNMQPYACVVVRDQAEREKFADAM 121
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BLAST of mRNA_E-fasciculatus_F_contig12131.1663.1 vs. uniprot
Match: D0NC21_PHYIT (Nitroreductase family, putative n=3 Tax=Phytophthora infestans TaxID=4787 RepID=D0NC21_PHYIT)

HSP 1 Score: 57.0 bits (136), Expect = 5.980e-6
Identity = 23/44 (52.27%), Postives = 33/44 (75.00%), Query Frame = 1 
Query:  604 PVPDDVLKKVLALVLGTPSGFNAQPYECIVVTSDEAKEKLSKAM 735
            PVPD+ LK++L L L  P+GFN QPY C++V   + +EKL++AM
Sbjct:   78 PVPDETLKEILKLTLRAPTGFNVQPYACVLVREPKDREKLAEAM 121
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BLAST of mRNA_E-fasciculatus_F_contig12131.1663.1 vs. uniprot
Match: A0A2D4CFG6_PYTIN (Nitroreductase family, putative n=2 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4CFG6_PYTIN)

HSP 1 Score: 57.4 bits (137), Expect = 6.560e-6
Identity = 30/66 (45.45%), Postives = 37/66 (56.06%), Query Frame = 1 
Query:  538 DAEAFERVTLARRSTQHFDRRRPVPDDVLKKVLALVLGTPSGFNAQPYECIVVTSDEAKEKLSKAM 735
            DA+   R  +A R+        PVPDDVL  VL L    PSGFN QPY C+VV     +E+L+ AM
Sbjct:   17 DAQEALRKLIASRTACRSFASDPVPDDVLADVLRLTQRAPSGFNMQPYACVVVREQADRERLADAM 82
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BLAST of mRNA_E-fasciculatus_F_contig12131.1663.1 vs. uniprot
Match: UPI00035D30CA (nitroreductase family protein n=1 Tax=Desulfobacter curvatus TaxID=2290 RepID=UPI00035D30CA)

HSP 1 Score: 55.8 bits (133), Expect = 7.410e-6
Identity = 24/58 (41.38%), Postives = 36/58 (62.07%), Query Frame = 1 
Query:  550 FERVTLARRSTQHFDRRRPVPDDVLKKVLALVLGTPSGFNAQPYECIVVTSDEAKEKL 723
            FE++T  RRS   FD  + +P +V+KK++ L   TPS FN QP+  +++     KEKL
Sbjct:    3 FEQITKDRRSINFFDPEKQIPQEVIKKMVELAGNTPSSFNLQPWNLMILQDKAQKEKL 60
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BLAST of mRNA_E-fasciculatus_F_contig12131.1663.1 vs. uniprot
Match: A0A6G0XIZ5_9STRA (Nitroreductase domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XIZ5_9STRA)

HSP 1 Score: 55.8 bits (133), Expect = 1.040e-5
Identity = 26/65 (40.00%), Postives = 39/65 (60.00%), Query Frame = 1 
Query:  541 AEAFERVTLARRSTQHFDRRRPVPDDVLKKVLALVLGTPSGFNAQPYECIVVTSDEAKEKLSKAM 735
            A+AF  +   RRS   + + +P+P ++L+ VL      PSGFN QPY C+VV   + + +LS AM
Sbjct:    2 AQAFRALARQRRSVYQY-QNKPIPKEILEDVLQTTQRAPSGFNLQPYACVVVQDPDERAQLSTAM 65
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BLAST of mRNA_E-fasciculatus_F_contig12131.1663.1 vs. uniprot
Match: A0A1F8QFT9_9CHLR (Nitroreductase domain-containing protein n=1 Tax=Chloroflexi bacterium RBG_16_57_11 TaxID=1797651 RepID=A0A1F8QFT9_9CHLR)

HSP 1 Score: 55.1 bits (131), Expect = 1.190e-5
Identity = 23/56 (41.07%), Postives = 42/56 (75.00%), Query Frame = 1 
Query:  568 ARRSTQHFDRRRPVPDDVLKKVLALVLGTPSGFNAQPYECIVVTSDEAKEKLSKAM 735
            +RRS +HF +++P+PDD++++VL      PS  NAQP+   V+TS E++++L++A+
Sbjct:    6 SRRSVRHF-QQKPIPDDIVRRVLETATWAPSAHNAQPWRFAVLTSLESRDRLAQAL 60
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BLAST of mRNA_E-fasciculatus_F_contig12131.1663.1 vs. uniprot
Match: A0A7S4JFU0_9STRA (Hypothetical protein n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4JFU0_9STRA)

HSP 1 Score: 55.5 bits (132), Expect = 1.650e-5
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 1 
Query:  550 FERVTLARRSTQHFDRRRPVPDDVLKKVLALVLGTPSGFNAQPYECIVVTSDEAKEKLSK 729
            F  +  +RRST+ FD  R +P  VL+ VL     +PSGFN QP + I+V S + K +LS+
Sbjct:   11 FRAIARSRRSTRRFDPGRSIPAPVLRDVLETTNTSPSGFNLQPTQVIIVRSPDLKSRLSR 70
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BLAST of mRNA_E-fasciculatus_F_contig12131.1663.1 vs. uniprot
Match: M4BXH7_HYAAE (Nitroreductase domain-containing protein n=1 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=M4BXH7_HYAAE)

HSP 1 Score: 54.7 bits (130), Expect = 3.220e-5
Identity = 23/43 (53.49%), Postives = 31/43 (72.09%), Query Frame = 1 
Query:  607 VPDDVLKKVLALVLGTPSGFNAQPYECIVVTSDEAKEKLSKAM 735
            VPD  L+K+L L L  P+GFN QPY C++V   + +EKLS+AM
Sbjct:   54 VPDKTLEKILKLTLRAPTGFNTQPYACVLVREQKDREKLSEAM 96
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BLAST of mRNA_E-fasciculatus_F_contig12131.1663.1 vs. uniprot
Match: A0A7S3PX76_9STRA (Hypothetical protein n=1 Tax=Chaetoceros debilis TaxID=122233 RepID=A0A7S3PX76_9STRA)

HSP 1 Score: 54.3 bits (129), Expect = 4.050e-5
Identity = 23/63 (36.51%), Postives = 40/63 (63.49%), Query Frame = 1 
Query:  541 AEAFERVTLARRSTQHFDRRRPVPDDVLKKVLALVLGTPSGFNAQPYECIVVTSDEAKEKLSK 729
            A+AF ++  +R S + F   RP+P ++L  +L   L +PSGFN QP   I++ + + K++L+K
Sbjct:    8 ADAFRKLAKSRHSCRRFQPNRPLPKEMLNDMLQTTLTSPSGFNIQPTNVIILQNQDIKQELAK 70
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig12131.1663.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 12
Match NameE-valueIdentityDescription
D7FT60_ECTSI7.000e-3391.67Nitroreductase-like protein n=1 Tax=Ectocarpus sil... [more]
K3WSR0_GLOUD4.400e-643.08Nitroreductase domain-containing protein n=1 Tax=G... [more]
D0NC21_PHYIT5.980e-652.27Nitroreductase family, putative n=3 Tax=Phytophtho... [more]
A0A2D4CFG6_PYTIN6.560e-645.45Nitroreductase family, putative n=2 Tax=Pythium in... [more]
UPI00035D30CA7.410e-641.38nitroreductase family protein n=1 Tax=Desulfobacte... [more]
A0A6G0XIZ5_9STRA1.040e-540.00Nitroreductase domain-containing protein n=1 Tax=A... [more]
A0A1F8QFT9_9CHLR1.190e-541.07Nitroreductase domain-containing protein n=1 Tax=C... [more]
A0A7S4JFU0_9STRA1.650e-543.33Hypothetical protein n=1 Tax=Odontella aurita TaxI... [more]
M4BXH7_HYAAE3.220e-553.49Nitroreductase domain-containing protein n=1 Tax=H... [more]
A0A7S3PX76_9STRA4.050e-536.51Hypothetical protein n=1 Tax=Chaetoceros debilis T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig12131contigE-fasciculatus_F_contig12131:1308..3382 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score129.8
Seed ortholog evalue1.3e-27
Seed eggNOG ortholog2880.D7FT60
EggNOG free text desc.Nitroreductase family
EggNOG OGs2RZ0D@2759,COG0778@1
Ec32 ortholog descriptionnitroreductase-like protein
Ec32 orthologEc-19_003470.2
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Exons3
Model size740
Cds size531
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig12131.1663.1prot_E-fasciculatus_F_contig12131.1663.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig12131 1515..3380 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927723.4374795-UTR-E-fasciculatus_F_contig12131:1307..15141622927723.4374795-UTR-E-fasciculatus_F_contig12131:1307..1514Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig12131 1308..1514 +
1695995666.36151-UTR-E-fasciculatus_F_contig12131:1307..15141695995666.36151-UTR-E-fasciculatus_F_contig12131:1307..1514Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig12131 1308..1514 +
1622927723.52341-UTR-E-fasciculatus_F_contig12131:3380..33821622927723.52341-UTR-E-fasciculatus_F_contig12131:3380..3382Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig12131 3381..3382 +
1695995666.400817-UTR-E-fasciculatus_F_contig12131:3380..33821695995666.400817-UTR-E-fasciculatus_F_contig12131:3380..3382Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig12131 3381..3382 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927723.467455-CDS-E-fasciculatus_F_contig12131:1514..16431622927723.467455-CDS-E-fasciculatus_F_contig12131:1514..1643Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12131 1515..1643 +
1695995666.3715029-CDS-E-fasciculatus_F_contig12131:1514..16431695995666.3715029-CDS-E-fasciculatus_F_contig12131:1514..1643Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12131 1515..1643 +
1622927723.4838636-CDS-E-fasciculatus_F_contig12131:2446..27401622927723.4838636-CDS-E-fasciculatus_F_contig12131:2446..2740Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12131 2447..2740 +
1695995666.3801231-CDS-E-fasciculatus_F_contig12131:2446..27401695995666.3801231-CDS-E-fasciculatus_F_contig12131:2446..2740Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12131 2447..2740 +
1622927723.5051858-CDS-E-fasciculatus_F_contig12131:3272..33801622927723.5051858-CDS-E-fasciculatus_F_contig12131:3272..3380Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12131 3273..3380 +
1695995666.3889503-CDS-E-fasciculatus_F_contig12131:3272..33801695995666.3889503-CDS-E-fasciculatus_F_contig12131:3272..3380Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12131 3273..3380 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig12131.1663.1

>prot_E-fasciculatus_F_contig12131.1663.1 ID=prot_E-fasciculatus_F_contig12131.1663.1|Name=mRNA_E-fasciculatus_F_contig12131.1663.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=177bp
MHVSTLHSTETSQTCCCEQLRPGGSCHTGHLLAPNALVGLLIQENAAAVF
CNQGVRFPVDPGVLQLLLHAARHSSTSSSLWIESGGTAGLPQESTAAAAA
AVAPLAAADSDAEAFERVTLARRSTQHFDRRRPVPDDVLKKVLALVLGTP
SGFNAQPYECIVVTSDEAKEKLSKAM*
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mRNA from alignment at E-fasciculatus_F_contig12131:1308..3382+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig12131.1663.1 ID=mRNA_E-fasciculatus_F_contig12131.1663.1|Name=mRNA_E-fasciculatus_F_contig12131.1663.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=2075bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12131:1308..3382+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GGGTCGTTTTCGGTGCTAGTGTACGGAATTCACCCTCTCTCCTGCTGCGA
TCTTCCGCGTGGTGTTATGTTGCTGCTGGTAGGCTGTACAGTAGTCCACC
TATCGAGAGGGAATGCTGGCGCTGCGCGCAGTTCACGGTTCACAGTCCCG
GAGGCGGCTGGGGCTAGAACCTAGAGCCTCAACGACGGAGCCCCCGGACG
AAGTTTCATGCACGTTTCCACATTGCACAGCACAGAGACCAGTCAGACCT
GTTGCTGTGAGCAGCTGCGTCCTGGAGGAAGTTGCCACACAGGACATCTT
CTGGCACCTAATGCGCTTGTTGGCCTATTGATACAGGTATGGGTAGTGGC
GCGGAGCACATCCAACATTCAACCAGGATAACACAAGTTGCACCGCGTCA
TGCGGCCACCGAGTTATTTGCTCAAGGGAGTGCTGGCTTCTCGGTGCCCA
CTTCTAATTTTACTGCTCTGCACACACACATGTGATCGTCTCTGCACGAG
TGTCGACGCACCGAGAGGTTTTAGTCGATATACTAAGAACACGGCATGTA
CGGAGAGGGCAGTATAGAGAGTTGGTGCGCCACTTCAGCAGTGCTGCACG
TTGCACTACTGCTGTGCTGTGGGGTTCATATGATGGGAGTACAATATCGT
ACAGCAGTAGTAGTCCAGGTCTTGTCTTACCCGGTTCAGGACACCGTTTC
AACTACACCACGAACGTTTTCGGCTCGGCCTGGGTTTCTCTGTTCGTGGG
TGAAAGTGTGTGTCCCCTTTCAAATAGCCCTGGTCGTGGAATGTTCTCGT
GCGAAAATTGGAGAGAGTTGCCGCGCTAAAAAACTGAACTTGTAGAGGAA
CTGCTAGCTGCTGTACGACTGGGCATAGGGTGCTTGCTGCAGTAGTCGGC
AAAACCACCCTGGCAGCTGCAACTATTTCTAGAGCTTTGAACGCGTGGCG
CCTGCGACGTAGCCAGCCTGCCCAAAGCACTAAACACGAGAGGCGTGGTC
TGCAGGCTTCCGCCCGCTTCTCTCTCTGTTCCATCAAACTATCGGGCGGT
TTCGACAAGAACTGCTGTCAGGCGCAGGCGTTGTTGTTCTTCCAACCCTC
CTGTCTACGTACTTCGTCGTACCACATGGTGGGCGCCAGGAAAATGCAGC
GGCTGTATTTTGTAATCAGGGCGTGCGTTTTCCTGTCGATCCCGGGGTTC
TCCAGCTCCTACTTCACGCCGCCCGCCACAGCAGCACCAGCTCGAGCCTC
TGGATCGAGAGCGGGGGCACCGCAGGGCTGCCGCAAGAGTCGACAGCAGC
AGCAGCAGCTGCAGTGGCCCCGCTCGCCGCTGCCGATAGCGATGCCGAAG
CGTTCGAGAGGGTGACCCTCGCCCGTCGCTCCACCCAGCACTTCGACCGA
CGACGACCGGTGCCGGACGACGTCTTGAAGAAGGTGATGCGCCTTGATTG
ATATTCCCGCCGGCCTTCCGAAGCCCCCAGGGTCGGGGTATATTTGAACG
TATACTGCCACTATGTAGGAAACAATGTGTAAGGCAGTACCATACGCAGC
TTCGCAAAGAAACAACGCCTGTTTGTGCTCGAGAAGGGTATCAATGTGGG
GTGGTCGAAAACTCTTGTCCTCCCGGTGGGAGTGCGCTCTTCATGTGGAT
GGCCTGCTTGACATGATTTGGTGTCGCTATTGCACACTGTTGTGGCTACA
TTCAGCAGGAGTTACAGGAGGGCCTTTCTAGTGAATCCGTAAGTCACAAG
CTCTGCTCGGTTTCCTCCATCTTGTCGACCGTGCCACTAATTCTGCTACT
TCGTCTGAACTCGCTTGGGTTTAGTGCACTTGTCAAGGTTTGGCCACAGC
AGTGTGCTCCGCTAGGGTTGTGAATTCCCTCTTTCTTTTTTCGTAAGACA
AGTGTCCTTTTTGCCTTCATGTTTTCACGCCTTGTCTCCGCGATTCCTTT
TTATTGTCTCGAAAGGTGTTGGCACTAGTCTTGGGAACGCCCTCCGGGTT
CAACGCCCAGCCGTACGAATGCATCGTGGTGACTTCCGACGAGGCCAAGG
AGAAGCTCAGCAAGGCCATGTGAGG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig12131:1308..3382+

>mRNA_E-fasciculatus_F_contig12131.1663.1 ID=mRNA_E-fasciculatus_F_contig12131.1663.1|Name=mRNA_E-fasciculatus_F_contig12131.1663.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=1062bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12131:1308..3382+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
ATGCACGTTTCCACATTGCACAGCACAGAGACCAGTCAGACCTGTTGCTG
TGAGCAGCTGCGTCCTGGAGGAAGTTGCCACACAGGACATCTTCTGGCAC
CTAATGCGCTTGTTGGCCTATTGATACAGATGCACGTTTCCACATTGCAC
AGCACAGAGACCAGTCAGACCTGTTGCTGTGAGCAGCTGCGTCCTGGAGG
AAGTTGCCACACAGGACATCTTCTGGCACCTAATGCGCTTGTTGGCCTAT
TGATACAGGAAAATGCAGCGGCTGTATTTTGTAATCAGGGCGTGCGTTTT
CCTGTCGATCCCGGGGTTCTCCAGCTCCTACTTCACGCCGCCCGCCACAG
CAGCACCAGCTCGAGCCTCTGGATCGAGAGCGGGGGCACCGCAGGGCTGC
CGCAAGAGTCGACAGCAGCAGCAGCAGCTGCAGTGGCCCCGCTCGCCGCT
GCCGATAGCGATGCCGAAGCGTTCGAGAGGGTGACCCTCGCCCGTCGCTC
CACCCAGCACTTCGACCGACGACGACCGGTGCCGGACGACGTCTTGAAGA
AGGAAAATGCAGCGGCTGTATTTTGTAATCAGGGCGTGCGTTTTCCTGTC
GATCCCGGGGTTCTCCAGCTCCTACTTCACGCCGCCCGCCACAGCAGCAC
CAGCTCGAGCCTCTGGATCGAGAGCGGGGGCACCGCAGGGCTGCCGCAAG
AGTCGACAGCAGCAGCAGCAGCTGCAGTGGCCCCGCTCGCCGCTGCCGAT
AGCGATGCCGAAGCGTTCGAGAGGGTGACCCTCGCCCGTCGCTCCACCCA
GCACTTCGACCGACGACGACCGGTGCCGGACGACGTCTTGAAGAAGGTGT
TGGCACTAGTCTTGGGAACGCCCTCCGGGTTCAACGCCCAGCCGTACGAA
TGCATCGTGGTGACTTCCGACGAGGCCAAGGAGAAGCTCAGCAAGGCCAT
GTGAGTGTTGGCACTAGTCTTGGGAACGCCCTCCGGGTTCAACGCCCAGC
CGTACGAATGCATCGTGGTGACTTCCGACGAGGCCAAGGAGAAGCTCAGC
AAGGCCATGTGA
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