mRNA_E_fasciculatus_S2_contig8634.16753.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig8634.16753.1
Unique NamemRNA_E_fasciculatus_S2_contig8634.16753.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig8634.16753.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G6G2_ECTSI)

HSP 1 Score: 239 bits (609), Expect = 2.570e-73
Identity = 106/133 (79.70%), Postives = 124/133 (93.23%), Query Frame = 3 
Query:   33 QGRQTTSVHVRMTSVADDQHWFKRAMKDYNETRASDLVVINFGARYHNTAEGDEAFKRDVFSVLDDMAEVGETATVVWREIAPTHFPAENGTYDAFVDIGLGNTACCTGTPVKVLDRNAWVQNYLRDNGLSDK 431
            +  QTTSVHVRMTS ADDQHW +RA++DYNETRASD+VV+NFGA Y +T EG+E+FKRDV S+LDDMAE+GETATVVWREIAPTHFPAENGTY+AFV++ LGNTACCTGTPVKVLDRNAWV++YLR+NGLSD+
Sbjct:  179 KANQTTSVHVRMTSAADDQHWLRRALRDYNETRASDVVVVNFGAHYRSTPEGEESFKRDVSSILDDMAEIGETATVVWREIAPTHFPAENGTYEAFVELDLGNTACCTGTPVKVLDRNAWVEDYLRENGLSDR 311
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BLAST of mRNA_E_fasciculatus_S2_contig8634.16753.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JEC7_9PHAE)

HSP 1 Score: 231 bits (589), Expect = 2.760e-69
Identity = 101/133 (75.94%), Postives = 121/133 (90.98%), Query Frame = 3 
Query:   33 QGRQTTSVHVRMTSVADDQHWFKRAMKDYNETRASDLVVINFGARYHNTAEGDEAFKRDVFSVLDDMAEVGETATVVWREIAPTHFPAENGTYDAFVDIGLGNTACCTGTPVKVLDRNAWVQNYLRDNGLSDK 431
            +  QTTSVHVRMTS ADD+HW +RA++DYNETRASD+VV+NFG  Y +T EG+E+FKRDV S+LDDMAE+GETATVVWREIAPTHFP ENGTY++FV++ LGN ACCTGTPVKVLDRNAWV++Y+RDNGLSD+
Sbjct:  268 KANQTTSVHVRMTSTADDRHWLRRALRDYNETRASDVVVVNFGPHYRSTPEGEESFKRDVSSILDDMAEIGETATVVWREIAPTHFPGENGTYESFVELDLGNNACCTGTPVKVLDRNAWVEDYVRDNGLSDR 400
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BLAST of mRNA_E_fasciculatus_S2_contig8634.16753.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LML8_ECTSI)

HSP 1 Score: 221 bits (562), Expect = 9.150e-69
Identity = 97/122 (79.51%), Postives = 114/122 (93.44%), Query Frame = 3 
Query:   66 MTSVADDQHWFKRAMKDYNETRASDLVVINFGARYHNTAEGDEAFKRDVFSVLDDMAEVGETATVVWREIAPTHFPAENGTYDAFVDIGLGNTACCTGTPVKVLDRNAWVQNYLRDNGLSDK 431
            MTS ADDQHW +RA++DYNETRASD+VV+NFGA Y +T EG+E+FKRDV S+LDDMAE+GETATVVWREIAPTHFPAENGTY+AFV++ LGNTACCTGTPVK LDRNAWV++YLR+NGLSD+
Sbjct:    1 MTSAADDQHWLRRALRDYNETRASDVVVVNFGAHYRSTPEGEESFKRDVSSILDDMAEIGETATVVWREIAPTHFPAENGTYEAFVELDLGNTACCTGTPVKFLDRNAWVEDYLRENGLSDR 122
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BLAST of mRNA_E_fasciculatus_S2_contig8634.16753.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXJ3_ECTSI)

HSP 1 Score: 173 bits (438), Expect = 6.640e-47
Identity = 78/128 (60.94%), Postives = 97/128 (75.78%), Query Frame = 3 
Query:   51 SVHVRMTSVADDQHWFKRAMKDYNETRASDLVVINFGARYHNTAEGDEAFKRDVFSVLDDMAEVGETATVVWREIAPTHFPAENGTYDAFVDI-GLGNTACCTGTPVKVLDRNAWVQNYLRDNGLSDK 431
            SVH+RM S    +HW  RA+ D+NETRASD+ V+NFG  YH+  E D++FKRDVF +LD+MA +GE ATVVWREIAPTHFPA NG++DAF  +      A CTGTP +V DRN WV+NY+RDNGL D+
Sbjct:  283 SVHMRMLSKPSGEHWLTRAVSDFNETRASDIFVVNFGGHYHDFPEEDDSFKRDVFPILDEMAVLGEKATVVWREIAPTHFPAVNGSFDAFDGLESKDRKASCTGTPPEVFDRNVWVENYVRDNGLEDR 410
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BLAST of mRNA_E_fasciculatus_S2_contig8634.16753.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KLA9_9PHAE)

HSP 1 Score: 171 bits (432), Expect = 6.080e-46
Identity = 78/128 (60.94%), Postives = 96/128 (75.00%), Query Frame = 3 
Query:   51 SVHVRMTSVADDQHWFKRAMKDYNETRASDLVVINFGARYHNTAEGDEAFKRDVFSVLDDMAEVGETATVVWREIAPTHFPAENGTYDAFVDI-GLGNTACCTGTPVKVLDRNAWVQNYLRDNGLSDK 431
            SVH+RM S    +HW  RA+ D+NETRASD+ V+NFG  YH+  E D++FKRDVF +LD+MA +GE ATVVWREIAPTHFPA NG++DAF  +      A CTGTP +V DRN WV+NYLR NGL D+
Sbjct:  294 SVHMRMLSKPSGEHWLTRAVSDFNETRASDIFVVNFGGHYHDFPEEDDSFKRDVFPILDEMAVLGEKATVVWREIAPTHFPAVNGSFDAFDCLESKDRKASCTGTPPEVFDRNVWVENYLRTNGLEDR 421
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BLAST of mRNA_E_fasciculatus_S2_contig8634.16753.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FKZ2_ECTSI)

HSP 1 Score: 161 bits (407), Expect = 2.470e-45
Identity = 72/125 (57.60%), Postives = 91/125 (72.80%), Query Frame = 3 
Query:   60 VRMTSVADDQHWFKRAMKDYNETRASDLVVINFGARYHNTAEGDEAFKRDVFSVLDDMAEVGETATVVWREIAPTHFPAENGTYDAFVDIGLGN-TACCTGTPVKVLDRNAWVQNYLRDNGLSDK 431
            +RM S    +HW  +A  D+NETRASD+ V+NFG  YH+  E DE FK D+  VL DMA +G+ ATV+WREIAPTHFPA NG+YD++ D+   + T  CTGTP KV DRN WV+NY+RDNGL D+
Sbjct:    1 MRMLSRPRGEHWLLKAASDFNETRASDVFVVNFGGHYHDIPEDDEEFKTDMIPVLQDMAVLGQNATVIWREIAPTHFPAANGSYDSYADLDAKDLTPRCTGTPPKVFDRNEWVENYVRDNGLEDR 125
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BLAST of mRNA_E_fasciculatus_S2_contig8634.16753.1 vs.
Match: Uncharacterized (protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FL04_ECTSI)

HSP 1 Score: 168 bits (425), Expect = 7.910e-45
Identity = 75/128 (58.59%), Postives = 94/128 (73.44%), Query Frame = 3 
Query:   51 SVHVRMTSVADDQHWFKRAMKDYNETRASDLVVINFGARYHNTAEGDEAFKRDVFSVLDDMAEVGETATVVWREIAPTHFPAENGTYDAFVDIGLGN-TACCTGTPVKVLDRNAWVQNYLRDNGLSDK 431
            SVH+RM S    +HW  +A  D+NETRASD+ V+NFG  YH+  E DE FK D+  VL DMA +G+ ATV+WREIAPTHFPA NG+YD++ D+   + T  CTGTP KV DRN WV+NY+RDNGL D+
Sbjct:  306 SVHMRMLSRPSGEHWLLKAASDFNETRASDVFVVNFGGHYHDIPEDDEEFKTDMIPVLQDMAVLGQNATVIWREIAPTHFPAANGSYDSYADLDAKDLTPRCTGTPPKVFDRNEWVENYVRDNGLEDR 433
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig8634.16753.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
Uncharacterized2.570e-7379.70protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
Uncharacterized2.760e-6975.94protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Uncharacterized9.150e-6979.51protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
Uncharacterized6.640e-4760.94protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
Uncharacterized6.080e-4660.94protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Uncharacterized2.470e-4557.60protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
Uncharacterized7.910e-4558.59protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FL04... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig8634contigE_fasciculatus_S2_contig8634:11..3295 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Stop1
Start1
Model size892
Hectar predicted targeting categoryother localisation
Exons4
Ec32 ortholog descriptionPC-Esterase
Ec32 orthologEc-05_003200.1
Cds size792
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig8634.16753.1prot_E_fasciculatus_S2_contig8634.16753.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig8634 76..3260 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842521.0737324-UTR-E_fasciculatus_S2_contig8634:10..751696842521.0737324-UTR-E_fasciculatus_S2_contig8634:10..75Ectocarpus fasciculatus EfasUO2UTRE_fasciculatus_S2_contig8634 11..75 +
1696842521.1191804-UTR-E_fasciculatus_S2_contig8634:3260..32951696842521.1191804-UTR-E_fasciculatus_S2_contig8634:3260..3295Ectocarpus fasciculatus EfasUO2UTRE_fasciculatus_S2_contig8634 3261..3295 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842521.0833693-CDS-E_fasciculatus_S2_contig8634:75..2781696842521.0833693-CDS-E_fasciculatus_S2_contig8634:75..278Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8634 76..278 +
1696842521.0920625-CDS-E_fasciculatus_S2_contig8634:1374..14951696842521.0920625-CDS-E_fasciculatus_S2_contig8634:1374..1495Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8634 1375..1495 +
1696842521.1007743-CDS-E_fasciculatus_S2_contig8634:1896..20111696842521.1007743-CDS-E_fasciculatus_S2_contig8634:1896..2011Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8634 1897..2011 +
1696842521.110395-CDS-E_fasciculatus_S2_contig8634:2907..32601696842521.110395-CDS-E_fasciculatus_S2_contig8634:2907..3260Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8634 2908..3260 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig8634.16753.1

>prot_E_fasciculatus_S2_contig8634.16753.1 ID=prot_E_fasciculatus_S2_contig8634.16753.1|Name=mRNA_E_fasciculatus_S2_contig8634.16753.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=264bp
MTSVADDQHWFKRAMKDYNETRASDLVVINFGARYHNTAEGDEAFKRDVF
SVLDDMAEVGETATVVWREIAPTHFPAENGTYDAFVDIGLGNTACCTGTP
VKVLDRNAWVQNYLRDNGLSDKGEAAAHLRHVHPAACRTPHLPPCPAFYH
GPGRGRKRLLPLLLRHRLQALVGDWRLRGVECAAAEPFMPDGIARLHLSS
EVQLSRSVEFCFRGCRNKEFREGFCPCFPSLLDTAYKNSVYFFGLQEFSP
PSSCGLTGQQQYL*
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mRNA from alignment at E_fasciculatus_S2_contig8634:11..3295+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig8634.16753.1 ID=mRNA_E_fasciculatus_S2_contig8634.16753.1|Name=mRNA_E_fasciculatus_S2_contig8634.16753.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=3285bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig8634:11..3295+ (Ectocarpus fasciculatus EfasUO2)
GGGGAGGAGGGGGGGGGGGAGGAGGAAACCACCAAGGGCGTCAAACAACG
TCGGTTCACGTGCGCATGACGTCTGTCGCGGACGACCAACACTGGTTCAA
GAGGGCCATGAAAGATTACAACGAGACGAGAGCGTCCGACCTCGTCGTGA
TCAACTTCGGCGCCCGCTACCACAACACCGCGGAGGGCGACGAGGCGTTC
AAGCGAGACGTGTTCTCTGTCCTAGATGATATGGCCGAGGTCGGCGAAAC
CGCCACCGTCGTTTGGAGGTATGCACAGGAAGAGCCGTCCGTGTTGTATT
CTTGTAGACCAGAGAGAGGTTTTACCAGCACTACTTGGTGTTGGTTTGAA
TGAACCCGGCGCTCAGTTCTCTTCGCCAACACTTTTGTAGGATGAGTGCT
TGAGTTCTGGTTTAGTGCCATGGTCAGAAGGTCTGCTGTGGCAGCTACCG
CAGTACACGCGCAGGGTTTGGTGCAAATCATCCCTCCTGCTCGGTACCAC
GTGTACCCTACAACGCAGGGCGTTCCAAGGTCAAGCGGGAGGGGTTTCCG
GTTTCGACTTGGGAAACGTACCACATACCTTATGGTAGCGACACCTGATC
TAGGGCCATATTTGGAAGGAGCTCTACGGCTCTTCAGTCGCGCAATGATT
TAGAGATTTTTGGGACTACAACGGGTTTGCAGCCTAGTTCCATCGCTGTA
GGTGCTGGCCTTTTTCTTGGCAACTGTCTTCCTCGTGTGCTCCATTGAAG
CACTGAACATGGTTGTAGCACCTCAGTAAGAGCGCCTTCAAATAACCGGC
TGCCTTGCTGAAGGCTGATGAAGTCGCTTGGCACGGCTTCTAGCGACTGT
CAAATCGAGCCCGTGACACCCTAGCTTCGAACTAGACATCTCGACCTCTT
GCTCCGTTCTGCCGGTTTGTAAAGGCCGGTGCCCGTTGGGACCCCGAAAT
CGCTCAGCACAATCGGGACAATAGACCATATTGTTGGACACAGTCGATAT
CGCTGTTCACGACAATGTCGCGGTGGTACCGCGCATAACACGGCTTGCAT
TTTTCTGCAGTCGTGTTGAGCTGTGCACAAAGGTCGAAACTCTCGTCACC
GTACGTAGTGAATGGTAGGAACATCAATGTAACCTGTTCCCTATATTCGT
CAGTGACGTAATGTTGCCCTTGACGCGTATTTCTTTATGCTCCTTGTGTT
TCGTTGTTTCTACTCACTACCGCATCTCAATGTCTTCTTCGATTTCACGA
CACGAGCAACGAATCGTGCTGGCACTTTTCAAGATGTCGCCTAATTGTGT
GTCTCTTCCCACCGATGTTGTTCCGATTGGCGTATCACGGTGCCGTGCCG
CCCACCTTTCGCAGAGAGATAGCACCGACTCACTTCCCTGCCGAAAACGG
CACGTATGATGCTTTCGTCGACATAGGCCTTGGCAACACCGCCTGCTGCA
CTGGAACGCCGGTGAAAGTTTTGGACAGAAATGCGGTGAGTGATTCCGCG
AAATAGGCAGTAAATCAGGTACAGCAGTAGCGTTTCTTTGTCAAGGAGGC
CCAAAACGATTTCGCGATGGACTTGTTTGCGAACGGTTCGTACGGGGTAC
CTACACGATGTTTGGGAACGATTGCACACGGGCTTGCCACGATGACTGCG
CTCGATGAAACTTTCAGACGTCATCACGCAGTCCTCTGGATGTACTTCTT
ATGCTGACAGGTAATTTTTGTTAAATGGTGATTGGACACAAGCGCCTTGC
CTGTTGGATGGACTAAATATGTCAACAACCAGTTTTGGTTGTGTGGCGAA
AATAACCGGCATCCTCACCGTTTCGCCATGATGACAGCAACAGCACCGAA
AGGTCTCTCCTCCACAATCGTGATATTTGCCAACAGTGGGTGCAGAACTA
CCTGCGGGACAATGGGCTTTCGGACAAGGGTGAAGCTGCTGCGCATTTAC
GACATGTCCATCCCGCGGCGTGCCGCACACCACACTTGCCACCTTGCCCC
GGTAAGAAGGGCCCATACCCGCTTTTTGGGTCTCCCACCTTTCTACAGGT
GTTTCTAAACCGCCCATGGGATACCCATAGGCCGCCCATGGGCATGACCA
TGGACTGCCCATGGAGCATGCCCGTGGGCGTGCCCATGGACAGCCCACGG
GTGGCCAATGAGTGCCCTTCACCCGGCAACAGTGTTTACTCCAAAACAAA
AGATTTCTACTTTGGAAGGGGCATTCCAACGGCAAATAACCCCTGACCCT
AACTGAAAGCCCCCACACGTGCTAGGTTGGTGCTGGGTGGCTAAGTTCTA
GGTTTATGCCGGTGGCGGTTCTAGGTTGAAGTGCCCACGGTTAACCGCCC
ACGGGCAGTTCGCAGCTCATGGGCTGCCCATGGGCGGCTCACGGGCAGTC
CATGAAGTGCCCATGGTGTCCCATGGGCGGCGTAGCGAAACTACGAGTAG
GGTGGGAGATAGTGTTTTTTGTTCCTAAGCACGCTTTACGGCTTCACCGC
ATCTCGCAAAAATCCATGCGTATCAGTTTGTCACACGGCCGCGAGGAGCT
TGCAGTACTTACAGTTATCGCGGTGAGACACGGCAGCGCTTTCTGCAACA
ATGCGGCGGCGTGAGACAGTGGACGAGTTACGGGGAGGCATCAGCAGATT
TTGAATTAAACGCCCTGCAGGCCGCATGCAAGGGTGGAACCCTCCCTCTA
AGGGGGGAACTGTACCTACTTTTCTGCTGGGGCCGGCGATAACATACTTT
ATTTGTGCCTACTTTTTAGTATGGTTGGCATGGAACTACATGAAAATATG
GCTGCAAGATGCAATCCGAACTATGTATGCACTACATCGTGGCAAGCGTG
TGCGGTGCTGTCGTTGTTTGTATTTATTTTTCCTCCTTCTGCTGCAGCCT
TCTACCATGGACCAGGCAGGGGCAGAAAGCGACTACTGCCACTCCTCCTA
CGGCACCGACTGCAGGCACTGGTCGGAGACTGGCGTTTAAGAGGGGTGGA
ATGCGCTGCTGCTGAACCATTTATGCCCGATGGCATAGCGAGGTTGCATT
TGTCAAGTGAAGTGCAGTTGTCTCGCAGTGTGGAGTTTTGCTTCCGCGGT
TGCCGCAACAAAGAGTTCAGGGAAGGTTTCTGCCCGTGCTTTCCTTCTCT
ACTCGACACTGCGTACAAAAACTCCGTGTATTTTTTCGGATTGCAAGAAT
TTTCACCACCGTCCTCTTGCGGATTGACGGGGCAACAGCAGTACCTGTGA
TGGTGCTCATGTTTCATAGTGGTTTTTCTGTTGTG
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig8634:11..3295+

>mRNA_E_fasciculatus_S2_contig8634.16753.1 ID=mRNA_E_fasciculatus_S2_contig8634.16753.1|Name=mRNA_E_fasciculatus_S2_contig8634.16753.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=792bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig8634:11..3295+ (Ectocarpus fasciculatus EfasUO2)
ATGACGTCTGTCGCGGACGACCAACACTGGTTCAAGAGGGCCATGAAAGA
TTACAACGAGACGAGAGCGTCCGACCTCGTCGTGATCAACTTCGGCGCCC
GCTACCACAACACCGCGGAGGGCGACGAGGCGTTCAAGCGAGACGTGTTC
TCTGTCCTAGATGATATGGCCGAGGTCGGCGAAACCGCCACCGTCGTTTG
GAGAGAGATAGCACCGACTCACTTCCCTGCCGAAAACGGCACGTATGATG
CTTTCGTCGACATAGGCCTTGGCAACACCGCCTGCTGCACTGGAACGCCG
GTGAAAGTTTTGGACAGAAATGCGTGGGTGCAGAACTACCTGCGGGACAA
TGGGCTTTCGGACAAGGGTGAAGCTGCTGCGCATTTACGACATGTCCATC
CCGCGGCGTGCCGCACACCACACTTGCCACCTTGCCCCGCCTTCTACCAT
GGACCAGGCAGGGGCAGAAAGCGACTACTGCCACTCCTCCTACGGCACCG
ACTGCAGGCACTGGTCGGAGACTGGCGTTTAAGAGGGGTGGAATGCGCTG
CTGCTGAACCATTTATGCCCGATGGCATAGCGAGGTTGCATTTGTCAAGT
GAAGTGCAGTTGTCTCGCAGTGTGGAGTTTTGCTTCCGCGGTTGCCGCAA
CAAAGAGTTCAGGGAAGGTTTCTGCCCGTGCTTTCCTTCTCTACTCGACA
CTGCGTACAAAAACTCCGTGTATTTTTTCGGATTGCAAGAATTTTCACCA
CCGTCCTCTTGCGGATTGACGGGGCAACAGCAGTACCTGTGA
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